Integrated device for hypoxia molding and feeding of zebra fish

Abstract

The utility model relates to a zebra fish hypoxia modeling and feeding integrated device. The device comprises a cylinder body, a hatching pond, a sealing plate, a nitrogen tank and an oxygen detector. A water outlet and an air outlet are formed in the side wall of the tank body. The hatching pond is detachably arranged at the top of the tank body, a detachable sealing cover is arranged at the top of the hatching pond, a coarse filtering net and a turnover fine filtering net are sequentially arranged in the hatching pond from top to bottom, and a water outlet is formed in the side wall of the hatching pond. The sealing plate is detachably arranged between the top of the cylinder body and the bottom of the hatching pond for sealing and isolation; and a nitrogen guide pipe is arranged on the nitrogen tank and extends into the cylinder body. And a probe of the oxygen detector extends into the cylinder body through the air outlet. The hatching pond and the tank body are vertically and integrally designed, and the feeding area and the anoxic modeling area are isolated through the detachable sealing plate, so that original external equipment can be omitted, and the occupied space of experimental equipment is reduced.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental device technology, specifically to an integrated device for feeding zebrafish in hypoxia modeling. Background Technology

[0002] Zebrafish, as an important model organism, are widely used in scientific research due to their rapid development, genetic similarity to humans, and transparent bodies that facilitate observation. Hypoxia models can simulate various pathological states, and creating hypoxia modeling devices can enhance the depth and efficiency of research.

[0003] Existing zebrafish hypoxia modeling equipment has limited functionality. Because the incubation and feeding system for brine shrimp, which serves as feed, is separate from the hypoxia modeling device, additional equipment such as brine shrimp hatching ponds, sorting filters, and collection boxes are required during experiments. This results in a large space occupation for the experimental equipment. Furthermore, after hatching, the brine shrimp need to pass through a filter outside the hatching pond to separate the shrimp shells and meat, and then be collected through an open collection box. This leads to the brine shrimp being exposed to the elements for a long time, which can easily affect the cleanliness of the feed and thus affect the stability of the experiment. Utility Model Content

[0004] The purpose of this invention is to provide an integrated feeding device for zebrafish in hypoxia modeling. By vertically integrating the hatching tank and the tank body, and separating the feeding and hypoxia modeling areas with a detachable sealing plate, it eliminates the need for external hatching tanks, filter sorting equipment, and collection boxes, reducing the space occupied by experimental equipment. By setting up a coarse filter screen to intercept shrimp shells and a flip-up fine filter screen to collect shrimp meat in the hatching tank, combined with the sealing design of the sealing cover and sealing plate, it can achieve a fully enclosed operation for shrimp egg hatching and shrimp shell separation, reducing the time that feed is exposed to the air environment and avoiding exposure to contamination.

[0005] To achieve the above objectives, this utility model provides an integrated device for feeding zebrafish in hypoxia modeling, comprising:

[0006] A tank for holding zebrafish, with a water outlet and an air outlet on the side wall of the tank.

[0007] The hatching pool is detachably mounted on the top of the tank. The top of the hatching pool is equipped with a detachable sealing cover. The hatching pool is equipped with a coarse filter screen and a flip-up fine filter screen in sequence from top to bottom. The hatching pool has a drain outlet on its side wall.

[0008] A sealing plate is detachably disposed between the top of the cylinder and the bottom of the hatching tank for sealing and isolation;

[0009] A nitrogen tank, wherein a nitrogen conduit is provided on the nitrogen tank and the nitrogen conduit leads into the cylinder body;

[0010] An oxygen detector, the probe of which extends into the cylinder through the outlet.

[0011] Optionally, a slot is provided on the top of the cylinder along the circumference, and a sealing rubber gasket is provided in the slot, with the bottom of the incubation pool being secured in the slot.

[0012] Optionally, sealing elongated holes are provided on both sides of the bottom of the incubation pool, and the sealing plate is disposed at the bottom of the incubation pool through the two sealing elongated holes.

[0013] Optionally, the drain outlet is located between the filter screen and the sealing plate and close to the sealing plate.

[0014] Optionally, the sealing plate is positioned at an angle downwards towards the drain outlet, forming an angle with the horizontal plane.

[0015] Optionally, a rotating handle connected to the filter screen is provided outside the incubation pool, and a connecting rod passing through the side wall of the incubation pool is provided between the rotating handle and the filter screen, and a sealed bearing is provided between the connecting rod and the side wall of the incubation pool.

[0016] Optionally, the height between the coarse filter screen and the sealing plate is greater than the width of the fine filter screen.

[0017] Optionally, the incubation pool is divided into two detachable support frames along the height direction, with the coarse filter screen disposed in one of the support frames and the fine filter screen disposed in the other support frame.

[0018] The beneficial effects of this utility model are as follows: by vertically integrating the hatching pool and the tank, and by isolating the feeding and hypoxic modeling areas with a detachable sealing plate, the original external hatching pool, filter sorting equipment, and collection box can be eliminated, reducing the space occupied by experimental equipment; by setting a coarse filter screen for intercepting shrimp shells and a flip-up fine filter screen for collecting shrimp meat in the hatching pool, combined with the sealing design of the sealing cover and sealing plate, a fully enclosed operation of shrimp egg hatching and shrimp shell separation can be achieved, reducing the time that feed is exposed to the air environment and avoiding exposure to pollution.

[0019] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0020] Figure 1 This is a schematic structural diagram of an integrated device for creating and feeding zebrafish in hypoxia, according to an embodiment of the present invention.

[0021] Figure 2 This is a schematic structural diagram of the slot of an integrated feeding device for creating an oxygen-deficient model of zebrafish, as shown in an embodiment of this utility model.

[0022] In the diagram: 1. Cylinder body; 11. Water outlet; 12. Air outlet; 13. Slot; 2. Hatching tank; 21. Sealing cover; 22. Coarse filter screen; 23. Fine filter screen; 24. Drain outlet; 25. Sealing elongated hole; 26. Rotating handle; 261. Connecting rod; 3. Sealing plate; 4. Nitrogen tank; 41. Nitrogen conduit; 5. Oxygen detector; 51. Probe. Detailed Implementation

[0023] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0024] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0025] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances. Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0026] Please see Figure 1A preferred embodiment of this application shows an integrated zebrafish hypoxia modeling and feeding device comprising a tank 1, a hatching pool 2, a sealing plate 3, a nitrogen tank 4, and an oxygen detector 5. The tank 1 is used to hold the zebrafish, and its side wall has a water outlet 11 and an air outlet 12. The hatching pool 2 is detachably mounted on the top of the tank 1, and a detachable sealing cover 21 is provided on the top of the hatching pool 2. A coarse filter 22 and a flip-up fine filter 23 are sequentially arranged from top to bottom inside the hatching pool 2. A drain outlet 24 is provided on the side wall of the hatching pool 2. The sealing plate 3 is detachably mounted between the top of the tank 1 and the bottom of the hatching pool 2 for sealing and isolation. A nitrogen conduit 41 is provided on the nitrogen tank 4, and the nitrogen conduit 41 leads into the tank 1. The probe 51 of the oxygen detector 5 extends into the tank 1 through the air outlet 12.

[0027] According to the embodiment of this utility model, by vertically integrating the hatching pool 2 and the tank 1, and by isolating the feeding and hypoxia-induced modeling areas with a detachable sealing plate 3, the original external hatching pool 2, filter sorting equipment, and collection box can be eliminated, reducing the space occupied by experimental equipment. By setting a coarse filter 22 for intercepting shrimp shells and a flip-up fine filter 23 for collecting shrimp meat in the hatching pool 2, combined with the sealing design of the sealing cover 21 and the sealing plate 3, a fully enclosed operation for shrimp egg hatching and shrimp shell separation can be achieved, reducing the time that feed is exposed to the air environment and avoiding exposure to pollution.

[0028] It should be noted that initially, both the sealing cover 21 and the sealing plate 3 are sealed to ensure the airtightness of the hatching tank 2. When feeding, first open the sealing cover 21 and add a certain volume of water, brine shrimp eggs, sodium chloride, and sodium bicarbonate to the hatching tank 2, ensuring the brine shrimp egg density is 2.4-5 g / L, the salinity is 25-35 g / L, and the pH is 7-7.5, while maintaining the room temperature at 26-28℃. The added brine shrimp eggs will be blocked by the coarse filter screen 22. After closing the sealing cover 21 and allowing it to stand for an appropriate time, the hatched brine shrimp meat will pass through the coarse filter screen 22 and deposit on the fine filter screen 23, while the shrimp shells will remain on the coarse filter screen 22. At this point, first open the drain outlet 24 on the hatching tank 2 to drain the water. Then open the sealing cover 21 and use a thin water pipe to rinse the coarse filter screen 22, washing away any remaining shrimp meat onto the fine filter screen 23. At the same time, rinse away any accumulated contaminants on the sealing plate 3 to prevent them from falling into the tank 1 and causing pollution. Then remove the sealing plate 3 and flip the fine filter screen 23 to allow the shrimp meat to fall into the tank 1 for feeding. Use the system water in the thin water pipe to rinse off any brine shrimp meat attached to the fine filter screen 23. Finally, replace the sealing plate 3 and the sealing cover 21 to complete the feeding process.

[0029] When setting up an oxygen-deficient environment, first open the air outlet 12 of tank 1 (the presence of air outlet 12 can prevent nitrogen from causing a rapid change in the tank pressure, creating an unsuitable living environment for the fish). Connect the oxygen detector 5 to the power supply and start it. Then open the nitrogen tank 4 to introduce nitrogen into tank 1, while paying attention to the oxygen concentration displayed on the oxygen detector 5 and adjusting the nitrogen flow rate to reach the set oxygen concentration. When tank 1 needs to be drained, open the water outlet 11 and slightly tilt the tank to drain the wastewater. During cleaning, the hatching tank 2 can be removed, and the coarse filter 22, fine filter 23, and tank 1 can be rinsed with electrolyte water.

[0030] The following detailed description uses specific examples:

[0031] Specifically, please see Figure 2 The top of the cylinder 1 has a circumferential groove 13, and a sealing rubber gasket is installed inside the groove 13. The bottom of the hatching pool 2 is locked inside the groove 13. Through the cooperation of the groove 13 and the sealing rubber gasket, the hatching pool 2 and the cylinder 1 can be quickly and tightly installed and disassembled, ensuring the sealing of the connection and simplifying the equipment assembly process.

[0032] Please see Figure 2 Specifically, the bottom of the incubation pool 2 has corresponding sealing elongated holes 25 on both sides, and the sealing plate 3 passes through the two sealing elongated holes 25 and is set at the bottom of the incubation pool 2. The design of the sealing elongated holes 25 allows the sealing plate 3 to be inserted horizontally and cover the channel between the incubation pool 2 and the tank 1, forming a physical isolation barrier, while also facilitating disassembly and maintenance.

[0033] Furthermore, the drain outlet 24 is located between the filter screen 23 and the sealing plate 3, and is close to the sealing plate 3. The sealing plate 3 is angled downwards towards the drain outlet 24 at an angle to the horizontal plane. Gravity guides the water flow and impurities to flow quickly along the inclined surface of the sealing plate 3 to the drain outlet 24, reducing dead zones during rinsing and preventing impurities from accumulating on the surface of the sealing plate 3.

[0034] Please see Figure 1 The hatching tank 2 is equipped with a rotating handle 26 connected to a fine filter screen 23. A connecting rod 261 passing through the side wall of the hatching tank 2 is provided between the rotating handle 26 and the fine filter screen 23. A sealed bearing is provided between the connecting rod 261 and the side wall of the hatching tank 2. The rotating handle 26 and the sealed bearing cooperate to enable manual operation of the fine filter screen 23 to be turned over for feeding shrimp meat, avoiding opening the hatching tank 2 and disrupting the closed environment, thus ensuring ease of operation and system sealing.

[0035] The height between the coarse filter 22 and the sealing plate 3 is greater than the width of the fine filter 23, which ensures that the fine filter 23 can be flipped smoothly to allow shrimp meat to be fed.

[0036] Furthermore, the incubation tank 2 is divided into two detachable support frames along its height. A coarse filter 22 is housed in one support frame, and a fine filter 23 is housed in the other. This modular design allows for the individual disassembly of the frame containing either the coarse or fine filter 23, facilitating targeted cleaning or replacement of the filters, preventing cross-contamination, and improving equipment maintenance efficiency and lifespan. It should be noted that because the fine filter 23 is flip-up, even if the incubation tank 2 was originally a single unit, flipping the fine filter 23 can create space, allowing impurities washed out by the coarse filter 22 to fall smoothly out of the incubation tank 2.

[0037] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0038] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A zebrafish hypoxia modeling and feeding integrated device, characterized in that, include: A tank for holding zebrafish, with a water outlet and an air outlet on the side wall of the tank. The hatching pool is detachably mounted on the top of the tank. The top of the hatching pool is equipped with a detachable sealing cover. The hatching pool is equipped with a coarse filter screen and a flip-up fine filter screen in sequence from top to bottom. The hatching pool has a drain outlet on its side wall. A sealing plate is detachably disposed between the top of the cylinder and the bottom of the hatching tank for sealing and isolation; A nitrogen tank, wherein a nitrogen conduit is provided on the nitrogen tank and the nitrogen conduit leads into the cylinder body; An oxygen detector, the probe of which extends into the cylinder through the outlet.

2. The zebrafish hypoxia modeling and feeding integrated device according to claim 1, characterized in that, The top of the cylinder has a groove along the circumference, and a sealing rubber gasket is provided in the groove. The bottom of the hatching pool is locked in the groove.

3. The zebrafish hypoxia modeling and feeding integrated device according to claim 1, characterized in that, The bottom of the incubation pool has corresponding sealing elongated holes on both sides, and the sealing plate passes through the two sealing elongated holes and is set at the bottom of the incubation pool.

4. The zebrafish hypoxia modeling and feeding integrated device according to claim 3, characterized in that, The drain outlet is located between the filter screen and the sealing plate and is close to the sealing plate.

5. The zebrafish hypoxia modeling and feeding integrated device according to claim 4, characterized in that, The sealing plate is angled downwards towards the drain outlet at an angle to the horizontal plane.

6. The zebrafish hypoxia modeling and feeding integrated device according to claim 1, characterized in that, A rotating handle connected to the filter screen is provided outside the incubation pool. A connecting rod passing through the side wall of the incubation pool is provided between the rotating handle and the filter screen. A sealed bearing is provided between the connecting rod and the side wall of the incubation pool.

7. The zebrafish hypoxia modeling and feeding integrated device according to claim 1, characterized in that, The height between the coarse filter screen and the sealing plate is greater than the width of the fine filter screen.

8. The zebrafish hypoxia modeling and feeding integrated device according to claim 1, characterized in that, The incubation pool is divided into two detachable support frames along the height direction. The coarse filter screen is installed in one of the support frames, and the fine filter screen is installed in the other support frame.

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