TIP head membrane puncturing needle for solving mixed spot blocking membrane, production mould of TIP head membrane puncturing needle and matched digestion filter plate fixing device

Abstract

The TIP head is vertically arranged, the tip end of the TIP head faces downwards, the middle of the TIP head is vertically inserted into the tip end of the TIP head, the outer side face of the middle of the TIP head is tightly matched with the inner side wall of the tip end of the TIP head, and the part, located below the tight matching position, of the TIP head is thinner than the part, located at the tight matching position, of the TIP head. The upper portion of the membrane puncturing needle is located in the TIP head, the sealing ring is horizontally arranged and located in the TIP head, the sealing ring is connected outside the upper portion of the membrane puncturing needle in a sleeved mode and abuts against the inner side wall of the TIP head, a groove for rotation is formed in the top face of the membrane puncturing needle, the lower portion of the membrane puncturing needle is located below the tip end of the TIP head, and the lower end of the lower portion of the membrane puncturing needle faces downwards and is gradually thinned inwards. The utility model further provides a related production mould and a digestion filter plate fixing device matched with the production mould. The membrane puncturing needle with the TIP head can puncture through an enrichment membrane and is matched with automatic DNA extraction equipment, so that the working efficiency is improved, and sample pollution is avoided.

Description

Technical Field

[0001] This utility model relates to the field of forensic DNA extraction technology, and particularly to the field of automated processing of mixed stain samples. Specifically, it refers to a TIP-tipped membrane puncture needle for solving the problem of mixed stain clogging, its production fixture, and a matching digestion and filter plate fixing device. Background Technology

[0002] Forensic DNA samples are extremely valuable in criminal investigations and judicial identification due to their uniqueness and irreplaceability. On-site DNA samples are usually "disposable," such as trace amounts of blood or contact biological traces. Once contaminated or lost, they cannot be reproduced. The value of the samples stems from their scientific value, legal validity, and irreversible extraction process. Their effective use requires advanced technology, professional competence, and rigorous procedures to ensure judicial fairness and investigative efficiency.

[0003] Mixed stains refer to biological samples formed by the mixture of bodily fluids or tissues from different individuals, such as a mixture of semen and vaginal fluid, or a mixture of blood or saliva from multiple individuals and skin cells. Mixed stains are mainly found in sex crime cases: vaginal swabs, underwear, toilet paper, etc., containing a mixture of sperm and vaginal epithelial cells.

[0004] Automated workstations are characterized by high efficiency, customization, and intelligence. When automating the processing of mixed stain samples, DNA typing of mixed semen and vaginal fluid stains is crucial for obtaining pure sperm cell DNA. Sperm cell nuclear membrane proteins are rich in disulfide cross-linked structures, exhibiting strong resistance to detergents and exogenous proteases. In contrast, vaginal epithelial cells can be digested using conventional detergents and proteases, often employing a two-step digestion method or differential extraction method.

[0005] An automated workstation already exists that separates male and female components using a separation membrane and an enrichment membrane. The enrichment membrane traps sperm cells, and the female components are filtered and transferred after centrifugation. The process for treating mixed stains is briefly described below:

[0006] 1. After processing the mixed stain sample, place it into a digestion filter plate with a separation membrane;

[0007] 2. Add an appropriate amount of digestive juice and gently digest at 56℃ for 60 minutes;

[0008] 3. The mixed stain sample was separated into solid and liquid components by centrifugation to obtain a liquid containing male and female components;

[0009] 4. The liquid is transferred to a digestive filter plate with an enrichment membrane, where the male components bind to the enrichment membrane;

[0010] 5. After centrifugation, the female components are filtered through an enrichment membrane and then transferred.

[0011] If there is a lot of vaginal fluid and vaginal epithelial cells in this step, the fluid is more viscous and may cause blockage of the enrichment membrane when passing through it. The female fluid cannot pass through the enrichment membrane normally and remains in the pores of the digestive filter plate. At this time, the male and female components cannot be separated normally.

[0012] 6. Automated detection to ensure that male and female components have been completely separated.

[0013] In this step, the automated equipment detects whether there is liquid in the pores of the digestion filter plate. If there is liquid, it means that the female component has not passed through the enrichment membrane smoothly, the male and female components have not been separated, and the membrane has been blocked by the female component. The automated equipment will report an error. At this time, it is necessary to manually use a clean, sharp object to pierce the enrichment membrane and manually transfer the liquid in the pores of the digestion filter plate.

[0014] 7. Rinse and concentrate the male components on the membrane to remove impurities;

[0015] 8. Add the lysis buffer for lysing male components into the pores of the digestion filter plate to lyse the male components;

[0016] 9. The lysis products of the male and female components were transferred to purification plates for adsorption, washing, and elution.

[0017] 10. Complete the extraction of male and female component DNA samples for the mixed spot experiment.

[0018] When processing mixed sample specimens automatically, if the enrichment membrane becomes clogged, the automated equipment will report a fault if it detects that the liquid has not been completely centrifuged. In this case, manual intervention is required. A sharp object such as a pin is used to puncture the enrichment membrane, allowing the female component to pass through the puncture and be centrifuged out before proceeding to the next step. This operation has several negative impacts, such as: the manual intervention is cumbersome; whether the sharp object used to puncture the enrichment membrane has been cleaned; the possibility of cross-contamination or external contamination during the operation; and the possibility of failed puncture attempts during manual operation.

[0019] Therefore, in order to address the situation where female components of mixed stain samples clog the enrichment membrane (step 6 of the mixed stain processing procedure above), it is desirable to provide a sharp object for solving the problem of mixed stain clogging the membrane, which can pierce the enrichment membrane and is compatible with automated DNA extraction equipment, thereby improving work efficiency and avoiding sample contamination. Utility Model Content

[0020] In order to overcome the shortcomings of the prior art, one objective of this utility model is to provide a TIP-tipped membrane piercing needle for solving the problem of mixed plaque blockage in membranes. This needle can penetrate enrichment membranes and is compatible with automated DNA extraction equipment, thereby improving work efficiency and avoiding sample contamination, making it suitable for large-scale application.

[0021] Another objective of this invention is to provide a production jig for TIP-head puncture needles used to solve mixed-spot membrane blockage. This jig can be used to produce TIP-head puncture needles for solving mixed-spot membrane blockage. It is ingeniously designed, has a simple structure, is easy to manufacture, and is suitable for large-scale promotion and application.

[0022] Another objective of this invention is to provide a digestion filter plate fixing device that works in conjunction with a TIP-tipped membrane needle for solving mixed plaque blockage. This device can fix a digestion filter plate with an enrichment membrane, thereby facilitating the use of the TIP-tipped membrane needle to pierce the enrichment membrane in the digestion filter plate and making it easier for the robotic arm module of automated DNA extraction equipment to pick up the digestion filter plate. This device is suitable for large-scale application.

[0023] To achieve the above objectives, in a first aspect of this utility model, a TIP-head puncture needle for solving mixed-plaque membrane blockage is provided, comprising a TIP head, wherein the TIP head is vertically arranged and the tip of the TIP head is downwardly positioned. The TIP-head puncture needle for solving mixed-plaque membrane blockage further includes a sealing ring and a puncture needle, wherein:

[0024] The puncture needle is vertically positioned, with its middle portion vertically inserted into the tip of the TIP head. The outer side of the middle portion of the puncture needle is tightly fitted with the inner wall of the tip of the TIP head. The portion of the puncture needle below the tight-fitting position is thinner than the portion of the puncture needle at the tight-fitting position. The upper portion of the puncture needle is located inside the TIP head. The sealing ring is horizontally positioned and located inside the TIP head, sleeved around the upper portion of the puncture needle and abutting against the inner wall of the TIP head. The top surface of the puncture needle has a rotating groove. The lower portion of the puncture needle is located below the tip of the TIP head, with the lower end of the lower portion of the puncture needle pointing downwards and gradually tapering inwards.

[0025] Preferably, the rotating groove is a straight groove, a cross-shaped groove, or a regular hexagonal groove.

[0026] Preferably, the upper part of the puncture needle includes a first cylinder, a second cylinder, and a third cylinder arranged coaxially from top to bottom. The diameter of the first cylinder is larger than the diameter of the second cylinder, and the diameter of the second cylinder is larger than the diameter of the third cylinder. The sealing ring is fitted over the third cylinder and abuts against the bottom surface of the second cylinder. The top surface of the first cylinder is provided with the rotation groove. The middle part of the puncture needle is an inverted frustum-shaped cone arranged coaxially with the third cylinder. The diameter of the top surface of the inverted frustum-shaped cone is equal to the diameter of the third cylinder. The lower part of the puncture needle is a fourth cylinder arranged coaxially with the third cylinder. The diameter of the bottom surface of the inverted frustum-shaped cone is equal to the diameter of the fourth cylinder.

[0027] Preferably, the puncture needle is a steel needle.

[0028] In a second aspect, this utility model provides a production fixture for the above-mentioned TIP-head puncture needle for solving mixed-spot membrane blockage, characterized in that it includes a base plate, a limiting plate, a left fixing plate, a right fixing block, a detection plate, and a detection circuit, wherein:

[0029] The base plate is horizontally positioned along the left-right direction. The left fixing plate is vertically positioned along the front-back direction on the left end of the base plate. The right fixing block is horizontally positioned along the front-back direction on the middle of the base plate. The limiting plate is horizontally positioned along the left-right direction on the base plate and located between the left fixing plate and the right fixing block. The detection plate is vertically positioned along the front-back direction on the right end of the base plate. The left fixing plate has a left insertion hole along the left-right direction. The top surface of the limiting plate has a limiting groove along the left-right direction. The right fixing block has a right insertion hole along the left-right direction. The left side of the detection plate has a detection area. The detection circuit is located inside the detection plate. The detection circuit has a detection contact and a detection indicator light. The detection contact is located within the detection area. The detection indicator light is located on the left side of the detection plate and outside the detection area. The left insertion hole and the right insertion hole are located to the left and right of the limiting groove, respectively. The detection contact is located to the right of the center position of the right insertion hole.

[0030] Preferably, both the left and right insertion holes are round holes, the detection area is a circular detection area, and the limiting groove is a semi-circular groove.

[0031] Preferably, the production fixture further includes an L-shaped fixing plate, which includes a horizontal plate and a vertical plate. The horizontal plate is disposed on the right end of the base plate along the front-back direction, and the vertical plate is disposed on the left side of the horizontal plate along the front-back direction. The detection plate is located to the left of the vertical plate and connected to the vertical plate.

[0032] In a third aspect of this utility model, a digestive filter plate fixing device is provided for use with the above-mentioned TIP head membrane-piercing needle for solving mixed plaque clogging of membranes. The device is characterized by comprising a rectangular frame and elastic latches. The rectangular frame is horizontally arranged along the left-right direction. The elastic latches are horizontally arranged and elastically extendable along the length direction of the elastic latches within the inner side of the rectangular frame. The tongue portion of the elastic latch protrudes from the inner side of the rectangular frame towards the space enclosed by the rectangular frame. Multiple elastic latches are provided, and these multiple elastic latches are horizontally arranged around the space enclosed by the rectangular frame at intervals.

[0033] Preferably, there are two elastic latches, a left elastic latch and a right elastic latch. The left elastic latch includes a left latch block and a left elastic element, and the right elastic latch includes a right latch block and a right elastic element, wherein:

[0034] A left groove is provided at the left rear corner of the inner side of the rectangular frame along a first inclined direction at a 45° angle to the rear frame plate of the rectangular frame. The left locking block is provided along the first inclined direction and is movably inserted into the left groove. The left elastic member is provided along the first inclined direction and is located in the left groove. The left elastic member is located between the bottom of the left groove and the left end of the left locking block and connects the bottom of the left groove and the left end of the left locking block respectively. The right end of the left locking block protrudes from the left groove towards the right front along the first inclined direction. The right end of the left locking block is a left tongue. The left tongue is concave to form a left rectangular notch. The two sides of the left rectangular notch are parallel to the rear frame plate and the left frame plate of the rectangular frame respectively.

[0035] A right groove is provided at the right front corner of the inner side of the rectangular frame along a second inclined direction at a 45° angle to the front frame plate of the rectangular frame. The right locking block is provided along the second inclined direction and is movably inserted into the right groove. The right elastic member is provided along the second inclined direction and is located in the right groove. The right elastic member is located between the bottom of the right groove and the right end of the right locking block and connects the bottom of the right groove and the right end of the right locking block respectively. The left end of the right locking block protrudes from the right groove to the left rearward along the second inclined direction. The left end of the right locking block is a right tongue. The right tongue is recessed to form a right rectangular notch. The two sides of the right rectangular notch are parallel to the front frame plate and the right frame plate of the rectangular frame respectively.

[0036] More preferably, the top surface of the right end of the left card block extends in an arc shape towards the lower right front along the first inclined direction to the left rectangular notch, and the bottom surface of the right end of the left card block extends in an arc shape towards the upper right front along the first inclined direction to the left rectangular notch; the top surface of the left end of the right card block extends in an arc shape towards the lower left rear along the second inclined direction to the right rectangular notch, and the bottom surface of the left end of the right card block extends in an arc shape towards the upper left rear along the second inclined direction to the right rectangular notch.

[0037] The main beneficial effects of this utility model are as follows:

[0038] 1. This utility model relates to a TIP-tipped membrane piercing needle for solving mixed plaque blockage of membranes. The needle comprises a TIP head, a sealing ring, and a piercing needle. The TIP head is vertically positioned with its tip pointing downwards. The piercing needle is also vertically positioned, with its middle portion vertically inserted into the tip of the TIP head. The outer side of the middle portion of the piercing needle is tightly fitted against the inner wall of the tip of the TIP head. The portion of the piercing needle below the tight-fitting position is thinner than the portion at the tight-fitting position. The upper portion of the piercing needle is located inside the TIP head. The sealing ring is horizontally positioned inside the TIP head, sleeved around the upper portion of the piercing needle and abutting against the inner wall of the TIP head. The top surface of the piercing needle has a rotating groove. The lower portion of the piercing needle is located below the tip of the TIP head, with its lower end pointing downwards and gradually tapering inwards. Therefore, it can pierce enrichment membranes and is compatible with automated DNA extraction equipment, thereby improving work efficiency and avoiding sample contamination, making it suitable for large-scale application.

[0039] 2. The production fixture for the TIP-head membrane-piercing needle of this utility model for solving mixed-spot membrane blockage includes a base plate, a limiting plate, a left fixing plate, a right fixing block, and a detection plate. The base plate is horizontally arranged along the left-right direction. The left fixing plate is vertically arranged along the front-back direction on the left end of the base plate. The right fixing block is horizontally arranged along the front-back direction on the middle of the base plate. The limiting plate is horizontally arranged along the left-right direction on the base plate and located between the left fixing plate and the right fixing block. The detection plate is vertically arranged along the front-back direction on the right end of the base plate. The left fixing plate has a left insertion hole along the left-right direction. The top surface of the limiting plate is horizontally arranged along the left-right direction. The device features a directional limiting groove, a right fixing block with a right insertion hole along the left-right direction, a detection area on the left side of the detection plate, and a detection circuit within the detection plate. The detection circuit includes detection contacts and a detection indicator light. The detection contacts are located within the detection area, while the detection indicator light is located on the left side of the detection plate outside the detection area. The left and right insertion holes are located to the left and right of the limiting groove, respectively, and the detection contacts are located to the right of the center of the right insertion hole. Therefore, it can be used to produce TIP-head membrane-piercing needles for solving mixed-spot membrane blockage. Its ingenious design, simple structure, and easy manufacturing make it suitable for large-scale application.

[0040] 3. The digestion filter plate fixing device of this utility model, used in conjunction with the TIP-tipped membrane piercing needle for solving mixed plaque blockage, includes a rectangular frame and elastic latches. The rectangular frame is horizontally arranged along the left-right direction, and the elastic latches are horizontally arranged and elastically extendable along the length of the elastic latches within the inner side of the rectangular frame. The tongue of the elastic latch protrudes from the inner side of the rectangular frame towards the space enclosed by the rectangular frame. There are multiple elastic latches, which are horizontally arranged around the space enclosed by the rectangular frame at intervals. Therefore, it can fix the digestion filter plate with enrichment membrane, thus facilitating the use of the TIP-tipped membrane piercing needle to pierce the enrichment membrane in the digestion filter plate, and making it easy for the robotic arm module of the DNA extraction automation equipment to grasp the digestion filter plate. It is suitable for large-scale application.

[0041] These and other objects, features and advantages of this utility model will be fully apparent from the following detailed description and drawings, and can be achieved by the means, devices and combinations thereof specifically pointed out in the description of the utility model. Attached Figure Description

[0042] Figure 1 This is a three-dimensional schematic diagram of a specific embodiment of the TIP-tipped membrane puncture needle of this utility model for solving mixed-spot membrane blockage.

[0043] Figure 2 This is a three-dimensional schematic diagram of a specific embodiment of the production jig for the TIP-head membrane-piercing needle of this invention for solving mixed-spot membrane blockage.

[0044] Figure 3 This is a perspective view of a specific embodiment of the digestive filter plate fixing device of the present invention, which is used in conjunction with the TIP head membrane-piercing needle for solving the problem of mixed plaque clogging of membranes.

[0045] Figure 4 yes Figure 3 A three-dimensional schematic diagram of the left card block of a specific embodiment is shown.

[0046] Figure 5 yes Figure 3 A three-dimensional schematic diagram of the right card block in a specific embodiment is shown.

[0047] Figure 6 yes Figure 3 The diagram shown is a three-dimensional representation of a specific embodiment in use.

[0048] (Symbol Explanation)

[0049] 1. TIP head for puncturing membranes to resolve mixed plaque blockage; 11. TIP head; 12. Puncture needle; 13. Rotation groove; 14. First cylinder; 15. Second cylinder; 16. Third cylinder; 17. Inverted frustum-shaped cone; 18. Fourth cylinder;

[0050] 2. Production fixture for TIP-head piercing needles used to solve mixed-spot membrane blockage; 201. Base plate; 202. Limiting plate; 203. Left fixing plate; 204. Right fixing block; 205. Detection plate; 206. Left insertion hole; 207. Limiting groove; 208. Right insertion hole; 209. Detection area; 210. L-shaped fixing plate; 211. Horizontal plate; 212. Vertical plate; 213. Detection indicator light;

[0051] 3. Digestive filter plate fixing device used in conjunction with TIP-head membrane puncture needles for resolving mixed plaque clogging; 31 Rectangular frame; 311 Left groove; 312 Right groove; 32 Elastic latch; 33 Left elastic latch; 331 Left locking block; 332 Left tongue; 333 Left rectangular notch; 334 Left slot; 34 Right elastic latch; 341 Right locking block; 342 Right tongue; 343 Right rectangular notch; 344 Right slot;

[0052] 4. Digestion filter plate. Detailed Implementation

[0053] In order to better understand the technical content of this utility model, the following embodiments are provided for detailed description.

[0054] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0055] Please see Figure 1 As shown, in a specific embodiment of the TIP-tipped membrane puncture needle of this invention for solving mixed-plaque membrane blockage, the TIP-tipped membrane puncture needle 1 of this invention includes a TIP head 11 (see...). Figure 2 ), sealing ring (not shown in the figure) and puncture needle 12, wherein:

[0056] The TIP head 11 is vertically positioned with its tip pointing downwards. The piercing needle 12 is also vertically positioned, with its middle portion vertically inserted into the tip of the TIP head 11. The outer side of the middle portion of the piercing needle 12 is tightly fitted to the inner wall of the tip of the TIP head 11. The portion of the piercing needle 12 below the tight-fitting position is thinner than the portion of the piercing needle 12 at the tight-fitting position. The upper portion of the piercing needle 12 is located inside the TIP head 11. The sealing ring is horizontally positioned and located inside the TIP head 11. The sealing ring is sleeved around the upper portion of the piercing needle 12 and abuts against the inner wall of the TIP head 11. The top surface of the piercing needle 12 is provided with a rotating groove 13. The lower portion of the piercing needle 12 is located below the tip of the TIP head 11, with the lower end of the lower portion of the piercing needle 12 pointing downwards and gradually tapering inwards.

[0057] The rotating groove 13 is used to insert tools such as screwdrivers for easy rotation, and can have any suitable shape. Preferably, the rotating groove 13 is a slotted groove, a cross-shaped groove, or a regular hexagonal groove. See also Figure 1 As shown, in a specific embodiment of the TIP-tipped membrane needle for solving mixed-spot clogging of the membrane according to the present invention, the rotating groove 13 is a straight groove.

[0058] The upper part, middle part, and lower part of the puncture needle 12 can have any suitable composition and shape; please refer to [link / reference]. Figure 1 As shown, in a specific embodiment of the TIP-tipped membrane puncture needle of this utility model for solving mixed-spot membrane blockage, the upper part of the puncture needle 12 includes a first cylinder 14, a second cylinder 15, and a third cylinder 16 arranged sequentially from top to bottom and coaxially. The diameter of the first cylinder 14 is larger than the diameter of the second cylinder 15, and the diameter of the second cylinder 15 is larger than the diameter of the third cylinder 16. The sealing ring is sleeved on the third cylinder 16 and abuts against the first cylinder 16. The bottom surface of the two cylinders 15, the top surface of the first cylinder 14 is provided with the rotation groove 13, the middle part of the piercing needle 12 is an inverted frustum 17 and is coaxially arranged with the third cylinder 16, the diameter of the top surface of the inverted frustum 17 is equal to the diameter of the third cylinder 16, the lower part of the piercing needle 12 is a fourth cylinder 18 and is coaxially arranged with the third cylinder 16, the diameter of the bottom surface of the inverted frustum 17 is equal to the diameter of the fourth cylinder 18.

[0059] The puncture needle 12 can be any suitable material. In a specific embodiment of the TIP-head puncture needle for solving mixed plaque blockage membrane in this invention, the puncture needle 12 is a steel needle.

[0060] Please see Figure 2 As shown, in a specific embodiment of the production fixture 2 for the TIP-head piercing needle of this utility model for solving mixed-spot membrane blockage, the production fixture 2 for the TIP-head piercing needle of this utility model includes a base plate 201, a limiting plate 202, a left fixing plate 203, a right fixing block 204, a detection plate 205, and a detection circuit, wherein:

[0061] The base plate 201 is horizontally positioned along the left-right direction. The left fixing plate 203 is vertically positioned along the front-back direction on the left end of the base plate 201. The right fixing block 204 is horizontally positioned along the front-back direction on the middle of the base plate 201. The limiting plate 202 is horizontally positioned along the left-right direction on the base plate 201 and located between the left fixing plate 203 and the right fixing block 204. The detection plate 205 is vertically positioned along the front-back direction on the right end of the base plate 201. The left fixing plate 203 has a left insertion hole 206 along the left-right direction. The top surface of the limiting plate 202 is positioned along the left-right direction... A limiting groove 207 is provided in the direction. The right fixing block 204 is provided with a right insertion hole 208 along the left and right directions. A detection area 209 is provided on the left side of the detection plate 205. The detection circuit is located in the detection plate 205. The detection circuit has a detection contact and a detection indicator light 213. The detection contact is located in the detection area 209. The detection indicator light 213 is located on the left side of the detection plate 205 and outside the detection area 209. The left insertion hole 206 and the right insertion hole 208 are located to the left and right of the limiting groove 207, respectively. The detection contact is located to the right of the center position of the right insertion hole 208.

[0062] The left insertion hole 206, the right insertion hole 208, the detection area 209, and the limiting groove 207 can have any suitable shape. Please refer to [link / reference]. Figure 2 As shown, in a specific embodiment of the production fixture of the TIP head piercing needle for solving the problem of mixed spot blockage of the membrane according to the present invention, the left insertion hole 206 and the right insertion hole 208 are both round holes, the detection area 209 is a circular detection area, and the limiting groove 207 is a semi-circular groove, that is, the cross-section of the limiting groove 207 is semi-circular.

[0063] The detection plate 205 is disposed on the right end of the base plate 201, and can adopt any suitable structure. Please refer to [link / reference]. Figure 2As shown, in a specific embodiment of the production fixture 2 for the TIP-head piercing needle for solving mixed-spot membrane blockage according to the present invention, the production fixture 2 for the TIP-head piercing needle for solving mixed-spot membrane blockage further includes an L-shaped fixing plate 210. The L-shaped fixing plate 210 includes a horizontal plate 211 and a vertical plate 212. The horizontal plate 211 is disposed on the right end of the base plate 201 along the front-back direction. The vertical plate 212 is disposed on the left side of the horizontal plate 211 along the front-back direction. The detection plate 205 is located to the left of the vertical plate 212 and connected to the vertical plate 212.

[0064] Please see Figures 3-5 As shown, in a specific embodiment of the digestive filter plate fixing device used in conjunction with the TIP head membrane-piercing needle for solving mixed plaque clogging of membranes according to this utility model, the digestive filter plate fixing device 3 used in conjunction with the TIP head membrane-piercing needle for solving mixed plaque clogging of membranes according to this utility model includes a rectangular frame 31 and an elastic latch 32. The rectangular frame 31 is horizontally arranged and arranged in the left-right direction. The elastic latch 32 is horizontally arranged and elastically retractable in the inner side of the rectangular frame 31 along the length direction of the elastic latch 32. The tongue of the elastic latch 32 protrudes from the inner side of the rectangular frame 31 toward the space enclosed by the rectangular frame 31. There are multiple elastic latches 32, which are arranged horizontally around the space enclosed by the rectangular frame 31 and spaced apart from each other.

[0065] The number of elastic latches 32 can be determined as needed; please refer to [link / reference]. Figures 3-5 As shown, in a specific embodiment of the digestive filter plate fixing device of this utility model used in conjunction with the TIP head puncture needle for solving mixed plaque clogging of membranes, the number of elastic latches 32 is two, namely a left elastic latch 33 and a right elastic latch 34. The left elastic latch 33 includes a left locking block 331 and a left elastic element (not shown in the figure), and the right elastic latch 34 includes a right locking block 341 and a right elastic element (not shown in the figure), wherein:

[0066] A left groove 311 is provided at the left rear corner of the inner side of the rectangular frame 31 along a first inclined direction at a 45° angle to the rear frame plate of the rectangular frame 31. A left locking block 331 is provided along the first inclined direction and is movably inserted into the left groove 311. A left elastic member is provided along the first inclined direction and is located in the left groove 311. The left elastic member is located between the bottom of the left groove 311 and the left end of the left locking block 331 and connects the bottom of the left groove 311 and the left end of the left locking block 331 respectively. The right end of the left locking block 331 protrudes from the left groove 311 towards the right front along the first inclined direction. The right end of the left locking block 331 is a left tongue 332. The left tongue 332 is recessed to form a left rectangular notch 333. The two sides of the left rectangular notch 333 are parallel to the rear frame plate and the left frame plate of the rectangular frame 31 respectively.

[0067] A right groove 312 is provided at the right front corner of the inner side of the rectangular frame 31 along a second inclined direction at a 45° angle to the front frame plate of the rectangular frame 31. A right locking block 341 is provided along the second inclined direction and is movably inserted into the right groove 312. A right elastic member is provided along the second inclined direction and is located in the right groove 312. The right elastic member is located between the bottom of the right groove 312 and the right end of the right locking block 341 and connects the bottom of the right groove 312 and the right end of the right locking block 341 respectively. The left end of the right locking block 341 protrudes from the right groove 312 to the left rearward along the second inclined direction. The left end of the right locking block 341 is a right tongue 342. The right tongue 342 is recessed to form a right rectangular notch 343. The two sides of the right rectangular notch 343 are parallel to the front frame plate and the right frame plate of the rectangular frame 31 respectively.

[0068] The left tongue portion 332 and the right tongue portion 342 can have any suitable shape; please refer to Figures 4-5 As shown, in a specific embodiment of the digestive filter plate fixing device of the present invention used in conjunction with the TIP head membrane needle for solving the problem of mixed plaque clogging, the top surface of the right end of the left locking block 331 extends in an arc shape towards the lower right along the first inclined direction to the left rectangular recess 333, and the bottom surface of the right end of the left locking block 331 extends in an arc shape towards the upper right along the first inclined direction to the left rectangular recess 333; the top surface of the left end of the right locking block 341 extends in an arc shape towards the lower left along the second inclined direction to the right rectangular recess 343, and the bottom surface of the left end of the right locking block 341 extends in an arc shape towards the upper left along the second inclined direction to the right rectangular recess 343.

[0069] The left and right elastic elements can be any suitable elastic elements. In a specific embodiment of the digestive filter plate fixing device of the present invention, which is used in conjunction with the TIP head puncture needle for solving the problem of mixed spot clogging of the membrane, both the left and right elastic elements are springs.

[0070] The left elastic element is connected to the left end of the left locking block 331, and the right elastic element is connected to the right end of the right locking block 341. Any suitable structure can be used; please refer to [link / reference needed]. Figures 4-5 As shown, in a specific embodiment of the digestive filter plate fixing device of the present invention used in conjunction with the TIP head membrane-piercing needle for solving the problem of mixed spot clogging, the left end of the left locking block 331 is provided with a left locking groove 334 along the first inclined direction, the right end of the left elastic member is inserted into the left locking groove 334 along the first inclined direction and connected to the bottom of the left locking groove 334, the right end of the right locking block 341 is provided with a right locking groove 344 along the second inclined direction, and the left end of the right elastic member is inserted into the right locking groove 344 along the second inclined direction and connected to the bottom of the right locking groove 344.

[0071] During the production of the TIP head piercing needle 1 for solving mixed spot blockage of the membrane according to this utility model, the TIP head 11 is placed in the left-right direction with the tip facing right. The TIP head 11 is placed to the left of the left fixing plate 203 of the production jig 2 for the TIP head piercing needle for solving mixed spot blockage of the membrane according to this utility model, so that the tip of the TIP head 11 is aligned with the left insertion hole 206. Then, the TIP head 11 is moved to the right so that the tip of the TIP head 11 passes through the left insertion hole 206 and the limiting groove 207 and is inserted into the right insertion hole 208 and abuts against the right insertion hole 208. At this time, the left end of the TIP head 11 is located in the left insertion hole 206 and the middle part is located in the limiting groove 207. The sealing ring is sleeved on the upper part of the piercing needle 12, and then... The puncture needle 12 is placed in the left-right direction with its tip facing right. The puncture needle 12 is placed to the left of the TIP head 11, and then the puncture needle 12 is moved to the right so that it enters the TIP head 11. A flathead screwdriver is inserted into the TIP head 11 and into the flat-shaped groove of the puncture needle 12 (if the rotation groove 13 is a flat-shaped groove). The puncture needle 12 is turned in the left-right direction and force is applied to the right. When the puncture needle 12 moves to the right and contacts the detection contact in the detection area 209, the detection circuit is turned on and the detection indicator light 213 lights up, indicating that the puncture needle 12 is installed in place. Immediately stop applying force. The installation of the TIP head puncture needle 1 for solving the problem of mixed spot blockage membrane is complete.

[0072] When using the TIP-tipped membrane piercing needle 1 of this invention to solve mixed-spot membrane blockage, if the enrichment membrane becomes blocked during automated processing of mixed-spot sample materials by an automated DNA extraction device, the pipetting module of the automated DNA extraction device (which loads a conventional TIP tip during normal liquid transfer) loads the TIP-tipped membrane piercing needle 1 of this invention. The loading method is the same as loading a conventional TIP tip. The blocked membrane pores are pierced, and then the female component is filtered through the pierced pores by centrifugation. The male component binds normally to the enrichment membrane, effectively removing the female component. This frees up the experimenter's hands and avoids the negative impact of manual intervention in fault recovery.

[0073] The TIP tip piercing needle 1 of this invention for solving mixed plaque blockage membranes has an impermeable structure. The pipetting module of the automated DNA extraction equipment can detect the pressure value after loading a normal aspiration TIP tip or the TIP tip piercing needle 1 of this invention through this impermeable structure. If the value is empty, it means that a normal aspiration TIP tip has been loaded. If the detected pressure value exceeds the set threshold, it means that the TIP tip piercing needle 1 of this invention has been loaded.

[0074] When the pipetting module of the automated DNA extraction equipment loads the TIP-tipped membrane-piercing needle 1 of this invention, which is used to solve mixed plaque blockage, and pierces the blocked pores, the pipetting module moves the TIP-tipped membrane-piercing needle 1 upward. The resistance between the piercing needle 12 and the membrane will cause the digestion filter plate 4 (see...) Figure 6 Therefore, a digestion filter plate fixing device is needed that meets two conditions: ① After the TIP head punctures the membrane at the membrane blockage site, it can effectively limit the digestion filter plate 4 so that the digestion filter plate 4 will not be lifted; ② When the robotic arm module of the automated DNA extraction equipment grasps the plate, it can also effectively grasp the digestion filter plate 4 without obstructing the digestion filter plate 4.

[0075] When using the digestive filter plate fixing device 3 of this invention, which is used in conjunction with the TIP head puncture needle for solving mixed plaque blockage membranes, the digestive filter plate 4 is placed above the rectangular frame 31 and moved downwards into the space enclosed by the rectangular frame 31. As it passes the elastic latch 32, the elastic latch 32 is elastically compressed and pushed into the inner side of the rectangular frame 31. After passing the elastic latch 32, the elastic latch 32 elastically extends back to its original shape, locking the bottom edge of the digestive filter plate 4 around its horizontal outward protrusion. Figure 6As shown, after the TIP head punctures the membrane at the pore location, the elastic latch 32 effectively limits the digestion filter plate 4, preventing it from being lifted. Then, the robotic arm module of the automated DNA extraction equipment grasps the digestion filter plate 4, lifts it upwards, and transfers it to another plate position. When passing the elastic latch 32, the elastic latch 32 is elastically compressed and pushed into the inner side of the rectangular frame 31. After passing the elastic latch 32, the elastic latch 32 elastically extends back to its original shape. Since the frictional force of the robotic arm module grasping the digestion filter plate 4 is greater than the limiting force of the elastic latch 32 on the digestion filter plate 4, the plate transfer is successful.

[0076] Therefore, the TIP-tipped membrane piercing needle of this invention for solving mixed-plaque membrane blockage can be manufactured using the production jig of the TIP-tipped membrane piercing needle of this invention. It can be loaded by the pipetting module of the automated DNA extraction equipment and can pierce the blockage holes of the digestion filter plate in the digestion filter plate fixing device used with the TIP-tipped membrane piercing needle of this invention. After piercing, the digestion filter plate can be picked up and transferred to other plate positions by the robotic arm module of the automated DNA extraction equipment, thereby solving many negative effects of manual membrane piercing, such as: cumbersome operation for operators to handle faults; whether the sharp objects used to pierce the enrichment membrane have been cleaned; whether cross-contamination or external contamination will occur during the operation; and the possibility of piercing failure during manual membrane piercing.

[0077] In summary, the TIP-tipped membrane piercing needle of this invention for solving mixed-plaque membrane blockage can penetrate the enrichment membrane and is compatible with automated DNA extraction equipment, thereby improving work efficiency and avoiding sample contamination. The production jig for the TIP-tipped membrane piercing needle for solving mixed-plaque membrane blockage can be used to produce the TIP-tipped membrane piercing needle itself, and is ingeniously designed, simple in structure, and easy to manufacture. The digestion filter plate fixing device used with the TIP-tipped membrane piercing needle can fix the digestion filter plate with the enrichment membrane, thus facilitating the penetration of the enrichment membrane in the digestion filter plate by the TIP-tipped membrane piercing needle and making it easy for the robotic arm module of the automated DNA extraction equipment to grasp the digestion filter plate, making it suitable for large-scale application.

[0078] Therefore, it is evident that the objective of this utility model has been fully and effectively achieved. The function and structural principles of this utility model have been demonstrated and explained in the embodiments. Without departing from the stated principles, any modifications can be made to the implementation methods. Therefore, this utility model includes all modified embodiments based on the spirit and scope of the claims.

Claims

1. A TIP head spike membrane needle for resolving mixed plaque occluded membrane, comprising a TIP head, the TIP head is vertically arranged, the tip of the TIP head is arranged downward, characterized in that, The TIP-head puncture needle for solving mixed-plaque membrane blockage also includes a sealing ring and a puncture needle, wherein: The puncture needle is vertically positioned, with its middle portion vertically inserted into the tip of the TIP head. The outer side of the middle portion of the puncture needle is tightly fitted with the inner wall of the tip of the TIP head. The portion of the puncture needle below the tight-fitting position is thinner than the portion of the puncture needle at the tight-fitting position. The upper portion of the puncture needle is located inside the TIP head. The sealing ring is horizontally positioned and located inside the TIP head, sleeved around the upper portion of the puncture needle and abutting against the inner wall of the TIP head. The top surface of the puncture needle has a rotating groove. The lower portion of the puncture needle is located below the tip of the TIP head, with the lower end of the lower portion of the puncture needle pointing downwards and gradually tapering inwards.

2. The TIP head spike membrane needle for resolving a mixed patch blocked membrane of claim 1, wherein, The rotating groove is a straight groove, a cross-shaped groove, or a regular hexagonal groove.

3. The TIP head spike membrane needle for resolving a mixed patch blocked membrane of claim 1, wherein, The upper part of the puncture needle includes a first cylinder, a second cylinder, and a third cylinder arranged coaxially from top to bottom. The diameter of the first cylinder is larger than the diameter of the second cylinder, and the diameter of the second cylinder is larger than the diameter of the third cylinder. The sealing ring is fitted around the third cylinder and abuts against the bottom surface of the second cylinder. The top surface of the first cylinder is provided with the rotation groove. The middle part of the puncture needle is an inverted frustum-shaped cone arranged coaxially with the third cylinder. The diameter of the top surface of the inverted frustum-shaped cone is equal to the diameter of the third cylinder. The lower part of the puncture needle is a fourth cylinder arranged coaxially with the third cylinder. The diameter of the bottom surface of the inverted frustum-shaped cone is equal to the diameter of the fourth cylinder.

4. The TIP head spike membrane needle for resolving a mixed patch blocked membrane of claim 1, wherein, The puncture needle is a steel needle.

5. A production mold for a TIP head spike membrane needle for resolving a mixed plaque clogging membrane according to claim 1, characterized in that, It includes a base plate, a limiting plate, a left fixing plate, a right fixing block, a detection plate, and a detection circuit, wherein: The base plate is horizontally positioned along the left-right direction. The left fixing plate is vertically positioned along the front-back direction on the left end of the base plate. The right fixing block is horizontally positioned along the front-back direction on the middle of the base plate. The limiting plate is horizontally positioned along the left-right direction on the base plate and located between the left fixing plate and the right fixing block. The detection plate is vertically positioned along the front-back direction on the right end of the base plate. The left fixing plate has a left insertion hole along the left-right direction. The top surface of the limiting plate has a limiting groove along the left-right direction. The right fixing block has a right insertion hole along the left-right direction. The left side of the detection plate has a detection area. The detection circuit is located inside the detection plate. The detection circuit has a detection contact and a detection indicator light. The detection contact is located within the detection area. The detection indicator light is located on the left side of the detection plate and outside the detection area. The left insertion hole and the right insertion hole are located to the left and right of the limiting groove, respectively. The detection contact is located to the right of the center position of the right insertion hole.

6. The production mold according to claim 5, wherein Both the left and right insertion holes are round holes, the detection area is a circular detection area, and the limiting groove is a semi-circular groove.

7. The production mold according to claim 5, wherein The production fixture also includes an L-shaped fixing plate, which includes a horizontal plate and a vertical plate. The horizontal plate is disposed on the right end of the base plate along the front-back direction, and the vertical plate is disposed on the left side of the horizontal plate along the front-back direction. The detection plate is located to the left of the vertical plate and connected to the vertical plate.

8. A digestive filter plate fixing device used in conjunction with the TIP-tipped membrane needle as described in claim 1 for resolving mixed plaque clogging of membranes, characterized in that, The device includes a rectangular frame and elastic latches. The rectangular frame is horizontally positioned along the left-right direction. The elastic latches are horizontally positioned and elastically extendable along the length of the elastic latches within the inner side of the rectangular frame. The tongue of the elastic latch protrudes from the inner side of the rectangular frame toward the space enclosed by the rectangular frame. There are multiple elastic latches, which are horizontally arranged around the space enclosed by the rectangular frame at intervals.

9. The digestion filter plate holder of claim 8, wherein, The number of elastic latches is two, namely a left elastic latch and a right elastic latch. The left elastic latch includes a left latching block and a left elastic element, and the right elastic latch includes a right latching block and a right elastic element, wherein: A left groove is provided at the left rear corner of the inner side of the rectangular frame along a first inclined direction at a 45° angle to the rear frame plate of the rectangular frame. The left locking block is provided along the first inclined direction and is movably inserted into the left groove. The left elastic member is provided along the first inclined direction and is located in the left groove. The left elastic member is located between the bottom of the left groove and the left end of the left locking block and connects the bottom of the left groove and the left end of the left locking block respectively. The right end of the left locking block protrudes from the left groove towards the right front along the first inclined direction. The right end of the left locking block is a left tongue. The left tongue is concave to form a left rectangular notch. The two sides of the left rectangular notch are parallel to the rear frame plate and the left frame plate of the rectangular frame respectively. A right groove is provided at the right front corner of the inner side of the rectangular frame along a second inclined direction at a 45° angle to the front frame plate of the rectangular frame. The right locking block is provided along the second inclined direction and is movably inserted into the right groove. The right elastic member is provided along the second inclined direction and is located in the right groove. The right elastic member is located between the bottom of the right groove and the right end of the right locking block and connects the bottom of the right groove and the right end of the right locking block respectively. The left end of the right locking block protrudes from the right groove to the left rearward along the second inclined direction. The left end of the right locking block is a right tongue. The right tongue is recessed to form a right rectangular notch. The two sides of the right rectangular notch are parallel to the front frame plate and the right frame plate of the rectangular frame respectively.

10. The digestion filter plate holding device as claimed in claim 9, wherein The top surface of the right end of the left card block extends in an arc shape towards the lower right along the first inclined direction to the left rectangular notch, and the bottom surface of the right end of the left card block extends in an arc shape towards the upper right along the first inclined direction to the left rectangular notch; the top surface of the left end of the right card block extends in an arc shape towards the lower left along the second inclined direction to the right rectangular notch, and the bottom surface of the left end of the right card block extends in an arc shape towards the upper left along the second inclined direction to the right rectangular notch.

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