Carrier

Abstract

The utility model relates to a carrier, which comprises a main body, the main body comprises a first layer and a second layer which are oppositely arranged, at least the second layer is a flexible film, the first layer and the second layer define an accommodating cavity, at least one connecting part is formed in the accommodating cavity, and the first layer and the second layer are connected through the connecting part. Therefore, the deformation of the accommodating cavity is reduced. The carrier is simple in structure and low in cost. Bubbles are not easy to form in the accommodating cavity. The thickness of the containing cavity is easy to control, and reaction samples are saved.

Description

Technical Field

[0001] This utility model relates to the field of in vitro detection technology, and in particular to a carrier. Background Technology

[0002] PCR (Polymerase Chain Reaction) is a molecular biology experimental method for the in vitro enzymatic synthesis of specific DNA fragments. It mainly consists of repeated thermal cycles of three steps: high-temperature denaturation, low-temperature annealing, and optimal extension. Before PCR amplification, the reaction sample needs to be placed in a vector. This sample consists of collected throat or nasal swabs and reagents used for PCR amplification. During PCR amplification, the reaction sample is heated by a heater and cooled by a cooling mechanism, thus cycling the reaction sample through the high-temperature denaturation, low-temperature annealing, and optimal extension stages.

[0003] Common carriers are tubular structures such as EP tubes. The diameter of the area where the reaction sample is held is large, and the heating and cooling rate and the homogenization rate of the reaction sample are slow. In the prior art, in order to improve the heating and cooling rate and the homogenization rate of the reaction sample, the carrier is made into a flat structure with one side of the carrier being an integrated heating structure and the other side being a cover plate placed on the heating structure. However, this type of carrier is expensive.

[0004] In addition, the reaction sample layer inside the flexible film carrier is very thin. Once there are bubbles, the bubbles are likely to penetrate the entire reaction sample layer in the thickness direction, which will affect the fluorescence detection results and the temperature uniformity of the reaction sample. However, how to eliminate bubbles in the carrier made of flexible film is a major problem.

[0005] In addition, existing technologies use flexible films such as PE films to prepare carriers to reduce costs and improve amplification efficiency. However, the problem with this method is that the strength of the flexible film is not enough, and the area of ​​the carrier that holds the reaction sample (hereinafter referred to as the liquid area) is prone to deformation. For example, the liquid area may collapse or bulge too high, and the thickness of the liquid area is difficult to control and unstable, resulting in poor detection stability.

[0006] In addition, the carrier in the existing technology has a large detectable area, and some areas are not detected during fluorescence detection, resulting in waste of reaction samples. Utility Model Content

[0007] One objective of this invention is to provide a carrier that at least solves one of the aforementioned technical problems.

[0008] A carrier includes a main body comprising a first layer and a second layer disposed opposite to each other, at least the second layer being a flexible film, the first layer and the second layer forming a receiving cavity, and at least one connecting portion formed within the receiving cavity, the connecting portion connecting the first layer and the second layer to reduce the deformation of the receiving cavity.

[0009] Optionally, the first layer is formed by vacuum forming, injection molding, or machining.

[0010] Optionally, a receiving groove is formed on the first layer, the receiving groove including a connecting groove and at least two sub-grooves, and two adjacent sub-grooves are connected through the connecting groove, wherein,

[0011] The connecting portion is provided on one side wall of the receiving groove, and the space between the connecting portion and the other side wall of the receiving groove forms the communicating groove; or

[0012] The receiving groove has connecting portions on both side walls, the connecting portions of the two side walls are arranged opposite to each other, and the space between the connecting portions of the two side walls forms the communicating groove; or

[0013] The two side walls of the receiving groove are provided with the connecting part, and the connecting parts of the two side walls are arranged alternately. The space of the receiving groove between the connecting part and the other side wall of the receiving groove forms the communicating groove.

[0014] In a top-view orientation, the cross-sectional area of ​​the connecting slot is smaller than the cross-sectional area of ​​the sub-slot; and / or

[0015] The connecting part is a column structure, one end of which is connected to the bottom of the receiving groove, and the other end extends toward the opening of the receiving groove. The column structure is located in the middle of the receiving groove.

[0016] Optionally, the first layer is a flexible film.

[0017] Optionally, the first layer has a lower deformability than the second layer.

[0018] Optionally, the first layer and the second layer are connected by laser welding, bonding or cryogenic bonding.

[0019] Optionally, the main body further includes a third layer, wherein the first layer is a flexible film, the deformability of the third layer is lower than that of the first layer and the second layer, the first layer and the second layer are respectively connected to each other on both sides of the thickness direction of the third layer, and a receiving groove is formed on the third layer that extends through its thickness direction, the receiving groove, the first layer and the second layer forming the receiving cavity; the connecting part extends from one side wall of the receiving groove to the other side wall to form the connecting part, and the connecting part is respectively connected to the first layer and the second layer.

[0020] Optionally, the receiving channel includes a connecting channel and at least two sub-channels, with two adjacent sub-channels connected by the connecting channel. One sidewall of the receiving channel is provided with the connecting portion, and the space between the connecting portion and the other sidewall of the receiving channel constitutes the connecting channel; or

[0021] The receiving channel has connecting portions on both side walls, the connecting portions of the two side walls are arranged opposite to each other, and the space between the connecting portions of the two side walls constitutes the communicating channel; or

[0022] The two side walls of the receiving channel are provided with the connecting part, and the connecting parts of the two side walls are arranged alternately. The space of the receiving channel between the connecting part and the other side wall of the receiving channel constitutes the communicating channel.

[0023] In a top-view orientation, the cross-sectional area of ​​the connecting channel is smaller than the cross-sectional area of ​​the sub-channel.

[0024] Optionally, the number of the connecting parts can be multiple.

[0025] The multiple connecting portions may have the same or different shapes; and / or

[0026] Multiple connecting portions are arranged along the length direction of the receiving cavity; and / or

[0027] The multiple connecting portions facing the sidewalls of the receiving cavity are arc-shaped; and / or

[0028] The connection points between the multiple connecting parts and the sidewalls of the receiving cavity are curved surfaces.

[0029] Optionally, the main body is provided with an inlet and / or an outlet communicating with the receiving cavity.

[0030] Optionally, both the sample inlet and the exhaust outlet are located in the first layer or the second layer; or

[0031] The injection port is located in one of the first layer and the second layer, and the exhaust port is located in the other of the first layer and the second layer.

[0032] Optionally, when the carrier includes a third layer, both the inlet and the outlet are located on the side of the third layer; or one of the inlet and the outlet is located on the side of the third layer, and the other is located in the first or second layer.

[0033] Optionally, the third layer is provided with an exhaust groove and / or a sample inlet groove, the exhaust groove and / or the sample inlet groove communicating with the receiving cavity and extending to the side of the third layer;

[0034] At least one of the first layer and the second layer forms the exhaust port with the wall of the exhaust groove, and / or at least one of the first layer and the second layer forms the injection port with the wall of the injection groove.

[0035] Optionally, the reaction sample enters the containment cavity through the inlet and the first end of the containment cavity, and the air in the containment cavity enters the exhaust port through the second end of the containment cavity, with the first end and the second end being arranged opposite to each other.

[0036] Optionally, the first end and the second end are located between the exhaust port and the sample inlet, with the sample inlet positioned closer to the first end and the exhaust port positioned closer to the second end; or

[0037] Both the exhaust port and the sample inlet are located on the side of the receiving cavity closer to the first end or closer to the second end.

[0038] Optionally, the sample inlet is disposed opposite to the first end of the receiving cavity, and the exhaust port is disposed opposite to the second end of the receiving cavity.

[0039] Optionally, the injection port and the receiving cavity are connected via an injection channel; and / or

[0040] The exhaust port is connected to the receiving cavity through an exhaust channel.

[0041] Optionally, one end of the injection channel is connected to the first end, and the other end is connected to the injection port; and / or

[0042] One end of the exhaust passage is connected to the second end, and the other end is connected to the exhaust port.

[0043] Optionally, when the main body includes a first layer, a second layer and a third layer, the third layer is provided with a flow channel groove and / or an exhaust channel groove, and the flow channel groove and / or the exhaust channel groove are both connected to the receiving channel. At least one of the first layer and the second layer and the groove wall of the exhaust channel groove form the exhaust channel, and / or at least one of the first layer and the second layer and the groove wall of the flow channel groove form the sample inlet channel.

[0044] When the main body includes a first layer and a second layer, the first layer has a flow channel groove and / or an exhaust channel groove, the second layer and the exhaust channel groove form the exhaust channel, and / or the second layer and the flow channel groove form the sample inlet channel; or, when the main body includes a first layer and a second layer, two spaced first connecting strips are formed between the first layer and the second layer, and the sample inlet channel is formed between the two first connecting strips; and / or, two spaced second connecting strips are formed between the first layer and the second layer, and the exhaust channel is formed between the two second connecting strips.

[0045] Optionally, the carrier further includes a sealing structure for restricting the flow of the reaction sample from the inlet and / or the exhaust port.

[0046] Optionally, the sealing structure includes a first sealing component capable of restricting the outflow of the reaction sample from the vent; and / or the sealing structure includes a second sealing component capable of sealing the inlet; or

[0047] The sealing structure includes a third sealing component capable of sealing the exhaust port and the sample inlet.

[0048] Optionally, the first sealing assembly includes a breathable but waterproof membrane that is sealed to the vent.

[0049] Optionally, the sealing structure can compress the air within the carrier and cause the first layer and / or the second layer to protrude outwards.

[0050] Optionally, the sealing structure includes:

[0051] An insert is sealed to the body and covers the exhaust port and / or the inlet port, and the insert has a sealed cavity communicating with the exhaust port and / or the inlet port;

[0052] A seal, at least a portion of which is capable of sealing an insertion into the sealing cavity to at least compress the air within the sealing cavity and to cause the first layer and / or the second layer to protrude outwards.

[0053] Optionally, the seal can seal the air inserted into the sealing cavity to compress the air within the sealing cavity and the receiving cavity.

[0054] Optionally, the thickness of the third layer is less than 0.5 mm, and / or

[0055] The third layer is made of polycarbonate, polypropylene, polyimide or polyethylene material, and / or

[0056] The third layer is made of transparent material.

[0057] Optionally, the thickness of the third layer is less than 0.3 mm.

[0058] Optionally, the thickness of the flexible film is less than 0.2 mm.

[0059] Optionally, the thickness of the flexible film is less than 0.1 mm or less than 0.05 mm.

[0060] Optionally, the first layer and / or the second layer may be made of a transparent material.

[0061] Optionally, the first layer and / or the second layer is a polycarbonate film, a polypropylene film, or a polyimide film; or

[0062] The first layer or the second layer is an aluminum film; or

[0063] The first layer or the second layer includes an aluminum film and a separator film, wherein the separator film is connected to the inner side of the aluminum film.

[0064] Optionally, the first layer and the third layer, as well as the second layer and the third layer, are connected by laser welding, bonding, or low-temperature bonding, and / or

[0065] The third layer is formed by machining or injection molding.

[0066] Optionally, the receiving cavity has a flat structure.

[0067] Optionally, the flat structure refers to a cavity whose dimension in the direction perpendicular to its thickness direction is greater than its dimension in the thickness direction.

[0068] Optionally, the ratio of the dimension of the receiving cavity in the direction perpendicular to its thickness direction to its dimension in the thickness direction is greater than 5:1.

[0069] Optionally, the size ratio is 50:1 to 100:1.

[0070] As can be seen from the above, the technical solution provided by this utility model includes a main body, which comprises a first layer and a second layer disposed opposite to each other. At least the second layer is a flexible film. The first layer and the second layer form a receiving cavity, and at least one connecting portion is formed within the receiving cavity. The first layer and the second layer are connected through the connecting portion to reduce the deformation of the receiving cavity. The carrier is formed by two layers, resulting in a simple structure and low cost. The first and second layers do not have a heating function, further reducing the cost of the carrier.

[0071] When the carrier comprises a main body, which consists of a first layer, a second layer, and a third layer, the carrier is formed by three layers, resulting in a simple structure and low cost. By creating a receiving groove in the third layer and sandwiching the third layer between the first and second layers, a receiving cavity for accommodating the reaction sample is formed by the first and second layers and the groove walls. The receiving groove is easy to process and shape. The first, second, and third layers are used to form the receiving cavity, further reducing the cost of the carrier. The third layer has lower deformability than the first and second layers, thereby increasing the overall strength of the carrier and preventing deformation.

[0072] This invention forms at least one connecting portion within the receiving cavity, connecting the first and second layers. Therefore, the connecting portion can prevent the surrounding first and second layers from bulging excessively or collapsing, thereby reducing the deformation of the receiving cavity and controlling its thickness. The number of connecting portions can be set according to the size of the receiving cavity, thus controlling the overall thickness of the cavity, making the thickness easy to control and stable, and improving the stability of the detection. The connecting portion occupies part of the space within the receiving cavity, effectively reducing the volume of the non-detection area within the cavity, reducing the amount of reaction sample required, and saving costs.

[0073] The main body is also provided with an exhaust port that communicates with the containment cavity. Therefore, when the sample is added through the injection port, the air in the containment cavity can be discharged in time through the exhaust port to prevent the formation of bubbles in the reaction sample, thereby avoiding affecting the fluorescence detection and improving the temperature uniformity of the reaction sample. Attached Figure Description

[0074] Figure 1a This is a cross-sectional view of the first carrier provided in Embodiment 1 of this utility model;

[0075] Figure 1b This is an exploded view of the first carrier provided in Embodiment 1 of this utility model;

[0076] Figure 1c This is a schematic diagram of the first layer corresponding to the first type of carrier provided in Embodiment 1 of this utility model;

[0077] Figure 1d This is a schematic diagram from another perspective of the first layer corresponding to the second type of carrier provided in Embodiment 1 of this utility model;

[0078] Figure 1e This is a schematic diagram of another first layer corresponding to the third carrier provided in Embodiment 1 of this utility model;

[0079] Figure 2 This is a perspective view of the third carrier provided in Embodiment 1 of this utility model;

[0080] Figure 3This is a perspective view of the fourth carrier provided in Embodiment 1 of this utility model;

[0081] Figure 4 This is a perspective view of the fifth carrier provided in Embodiment 1 of this utility model;

[0082] Figure 5a This is a schematic diagram of the carrier without the injected reaction sample provided in Embodiment 1 of this utility model;

[0083] Figure 5b This is a schematic diagram of the carrier injection into the reaction sample provided in Embodiment 1 of this utility model;

[0084] Figure 5c This is a schematic diagram of the carrier for injecting reaction samples and the sealing structure for sealing the inlet and outlet provided in Embodiment 1 of this utility model;

[0085] Figure 6A This is a front view of the first carrier provided in Embodiment 2 of this utility model;

[0086] Figure 6B This is a cross-sectional view (along the second embodiment of the present invention) of the first type of carrier. Figure 6A (Central AA direction)

[0087] Figure 6C This is a front view of the first layer of the first carrier provided in Embodiment 2 of this utility model;

[0088] Figure 7 This is a front view of the second carrier provided in Embodiment 2 of this utility model;

[0089] Figure 8 This is a front view of the first layer of the second carrier provided in Embodiment 2 of this utility model;

[0090] Figure 9 This is a cross-sectional view of the third carrier provided in Embodiment 2 of this utility model;

[0091] Figure 10 This is a schematic diagram of the structure of the first carrier provided in Embodiment 3 of this utility model;

[0092] Figure 11 This is an exploded view of the second carrier provided in Embodiment 3 of this utility model;

[0093] Figure 12 This is a schematic diagram of the third layer corresponding to the second carrier provided in Embodiment 3 of this utility model;

[0094] Figure 13 This is a schematic diagram of another third layer corresponding to the second carrier provided in Embodiment 3 of this utility model;

[0095] Figure 14This is a perspective view of the second carrier provided in Embodiment 3 of this utility model;

[0096] Figure 15 This is a perspective view of the third carrier provided in Embodiment 3 of this utility model;

[0097] Figure 16 This is a perspective view of the fourth carrier provided in Embodiment 3 of this utility model;

[0098] Figure 17 This is an exploded view of the fourth carrier provided in Embodiment 3 of this utility model;

[0099] Figure 18 This is an exploded view of the fifth carrier provided in Embodiment 3 of this utility model;

[0100] Figure 19 This is an exploded view of the sixth carrier provided in Embodiment 3 of this utility model;

[0101] Figure 20 This is a schematic diagram of the third layer corresponding to the sixth carrier provided in Embodiment 3 of this utility model.

[0102] In the picture:

[0103] Example 1

[0104] 1. Main body; 11. First layer; 111. Connecting part; 112. Recess; 113. Receiving groove; 1131. Connecting groove; 1132. Sub-groove; 114. Flow channel groove; 115. Exhaust channel groove; 12. Second layer; 13. Receiving cavity; 131. First end; 132. Second end; 14. Sample inlet; 15. Exhaust port; 16. Sample inlet channel; 161. First connecting strip; 17. Exhaust channel; 171. Second connecting strip;

[0105] 3. Third sealing assembly; 31. Seal; 32. Inserted part; 321. Sealing cavity; 322. Injection hole; 323. Vent hole.

[0106] Example 2

[0107] 2. Main body; 21. First layer; 22. Second layer; 23. Receiving cavity; 24. Sample inlet; 25. Exhaust port; 26. Sample inlet channel; 27. Exhaust channel; 211. Connecting part; 213. Receiving groove; 2131. Connecting groove; 2132. Sub-groove; 214. Flow channel groove; 215. Exhaust channel groove.

[0108] Example 3

[0109] 6. Main body; 61. First layer; 62. Second layer; 63. Receiving cavity; 631. First end; 632. Second end; 64. Sample inlet; 65. Exhaust port; 66. Sample inlet channel; 67. Exhaust channel; 68. Third layer; 681. Receiving groove; 682. Exhaust groove; 683. Sample inlet; 684. Flow channel; 685. Exhaust channel; 686. Connecting part;

[0110] 3. Third sealing assembly; 31. Seal; 32. Inserted part; 321. Sealing cavity; 322. Injection port; 323. Vent port;

[0111] 4. Second sealing assembly; 5. First sealing assembly; 51. Breathable but waterproof membrane. Detailed Implementation

[0112] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this utility model and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this utility model are shown in the accompanying drawings, not all of them.

[0113] This utility model defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up", "down", "left", "right", "inner", and "outer", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this utility model.

[0114] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0115] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0116] This embodiment provides a vector for carrying reaction samples, such as reaction samples that require PCR amplification, but not limited to this, in order to reduce the cost of the vector.

[0117] Example 1

[0118] like Figures 1a-5c As shown, this embodiment provides a carrier including a main body 1. The main body 1 includes a first layer 11 and a second layer 12 disposed opposite to each other. The second layer 12 is a flexible film. The deformation capacity of the first layer is less than that of the second layer 12. The first layer 11 and the second layer 12 are connected to form a receiving cavity 13. At least one connecting portion 111 is formed in the receiving cavity 13. The connecting portion 111 connects the first layer 11 and the second layer 12 to reduce the deformation of the receiving cavity 13. In this embodiment, the connecting portion 111 is integrally formed with the first layer. In other embodiments, the connecting portion 111 can also be connected to the first layer 11 and the second layer 12 by, for example, dispensing or double-sided adhesive.

[0119] It is understood that since the connecting part 111 is formed in the receiving cavity 13 and the connecting part 111 connects the first layer 1 and the second layer 12, in this embodiment, the portion of the receiving cavity 13 other than the portion 111 can accommodate the reaction sample, while the position of the receiving cavity 13 where the connecting part 111 is formed cannot accommodate the reaction sample.

[0120] Because the cross-sectional area of ​​the receiving cavity 13 parallel to the thickness direction of the carrier is large, and the second layer 12 is a flexible film, the receiving cavity 13 is prone to deformation after bearing the reaction sample, making it difficult to control and resulting in unstable thickness, leading to poor detection stability. In this embodiment, by forming at least one connecting portion 111 in the receiving cavity 13, the first layer 11 and the second layer 12 are connected through the connecting portion 111. Therefore, the connecting portion 111 can limit the bulging or collapse of the surrounding first layer 1 and second layer 12, thereby reducing the deformation of the receiving cavity 13 and controlling its thickness. The number of connecting portions 111 can be set according to the size of the receiving cavity 13, thereby controlling the overall thickness of the receiving cavity 13, making the thickness of the receiving cavity 13 easy to control and stable, and improving the stability of the detection.

[0121] The carrier provided in this embodiment is formed by two layers. The carrier has a simple structure and low cost.

[0122] like Figures 1b-1cAs shown, the number of connecting parts 111 is at least two. The more connecting parts 111 there are, the easier it is to control the liquid layer thickness within the receiving cavity 13. However, too many connecting parts 111 may cause difficulties in liquid injection. Therefore, the number of connecting parts 111 can be determined according to specific circumstances. The shapes of the connecting parts 111 may be the same or different, depending on needs or ease of processing. Optionally, multiple connecting parts 111 are provided along the length of the receiving cavity 13, thereby ensuring a uniform thickness distribution within the receiving cavity 13.

[0123] In this embodiment, the second layer 12 is a flexible film, and the deformability of the first layer 11 is lower than that of the second layer 12. At this time, the first layer 11 is not a flexible film, but the first layer 11 can improve the strength and stiffness of the entire carrier. For example, the first layer 11 can be a support layer or substrate with greater stiffness and / or thickness than the second layer 12.

[0124] like Figures 1a-1c As shown, when the deformability of the first layer 11 is lower than that of the second layer 12, such as when the thickness of the first layer 11 is greater than that of the second layer 12, a receiving groove 113 is formed on the first layer 11. The receiving groove 113 includes a connecting groove 1131 and at least two sub-grooves 1132, and two adjacent sub-grooves 1132 are connected through the connecting groove 1131. A connecting portion 111 extends from one sidewall of the receiving groove 113 to the other sidewall, and the space between the connecting portion 111 and the other sidewall of the receiving groove 113 forms the connecting groove 1131. In the top view, the cross-sectional area of ​​the connecting groove 1131 is smaller than the cross-sectional area of ​​the sub-grooves 1132 (or in other words, the cross-section is the cross-section of the connecting groove 1131 or the sub-grooves 1132 in the thickness direction of the first layer 11). The space enclosed by the connecting groove 1131 and the sub-groove 1132 and the second layer 12 is used to accommodate the reaction sample. The connecting part 111 formed by the side wall of the connecting groove 1131 makes the cross-sectional area of ​​the connecting groove 1131 smaller than the cross-sectional area of ​​the sub-groove 1132. The change in the cross-sectional area of ​​the connecting groove 1131 and the sub-groove 1132 indicates the change in the cross-sectional area of ​​the space accommodating the reaction sample. When the cross-sectional area becomes smaller, it can limit the excessive protrusion or collapse of the accommodating cavity 13.

[0125] like Figure 1c As shown, when both side walls of the receiving groove 113 are provided with connecting parts 111, the connecting parts 111 of the two side walls are arranged alternately, and the space of the receiving groove 113 between the connecting part 111 and the other side wall of the receiving groove 113 forms a communicating groove 1131.

[0126] After the first layer 11 has a receiving cavity 113, the thickness of the area where the first layer 11 and the receiving cavity 13 are directly opposite is small. When the heater and cooler are used to heat the carrier, heat can be quickly transferred to the reaction sample through the first layer 11 and the second layer 12, which can realize rapid heating and cooling of the reaction sample and shorten the PCR reaction time.

[0127] like Figure 1d As shown, the deformability of the first layer 11 is lower than that of the second layer 12, and the thickness of the first layer 11 is greater than that of the second layer 12. A receiving groove 113 is formed on the first layer 11. The receiving groove 113 includes a connecting groove 1131 and at least two sub-grooves 1132. Two adjacent sub-grooves 1132 are connected by the connecting groove 1131. As shown, protrusions extending from the two sidewalls of the receiving groove 113 in opposite directions form connecting portions 111, and the connecting groove 1131 is formed between two connecting portions 111. In a top view, the cross-sectional area of ​​the connecting groove 1131 is smaller than the cross-sectional area of ​​the sub-grooves 1132 (or the cross-section is the cross-section of the connecting groove 1131 or the sub-grooves 1132 in the thickness direction of the first layer 11). The space enclosed by the connecting groove 1131, the sub-grooves 1132, and the second layer 12 is used to accommodate the reaction sample.

[0128] like Figure 1e As shown, the connecting part 111 is a columnar structure. One end of the columnar structure is connected to the bottom of the receiving groove 113, and the other end extends towards the opening of the receiving groove 113. The columnar structure is located in the middle of the receiving groove. It can be understood that the columnar structure being located in the middle of the receiving groove 113 means that the columnar structure does not contact the side wall of the receiving groove 111, but it is not limited to the columnar structure being located at the midpoint of the receiving groove. Optionally, the connecting part 111 can be integrally formed with the first layer 11.

[0129] The columnar structure supports the second side 12 in the middle of the cavity 113, further improving the thickness uniformity of the entire cavity 113 (which carries the reaction sample).

[0130] Optionally, the height of the surface where the connecting part 111 connects to the second layer 12 is flush with the surface of the first layer 11 where the receiving groove 113 is not opened, thereby effectively supporting the second layer 12 and reducing the deformation of the receiving cavity 13.

[0131] like Figure 1c , 1d Optionally, from a top view, the connecting part 111 is connected to the receiving cavity 113, and the outer edge of the connecting part 111 is an arc surface. Liquid flow exhibits a wall-adhering effect. When the outer edge of the connecting part 111 is an arc surface, it facilitates better flow of the reaction liquid within the receiving cavity 13, and also prevents the formation of bubbles in the reaction sample as it flows through the connecting part 111. Simultaneously, it also prevents air entrapment at the aforementioned arc surface during the injection molding of the first layer 11. In contrast, if the outer edge of the connecting part 111 is a right-angled surface, the wall-adhering effect is poor when the reaction liquid flows to the right-angle position, and bubbles will form due to impact during the flow of the reaction liquid. The connection between the connecting part 111 and the side wall of the receiving cavity 13 is an arc surface, such as... Figure 1cAs shown, the connecting part 111 is in a "ji" shape, and its protruding end is in an arc shape. The connection between the connecting part 111 and the side wall of the accommodating cavity 13 is in a tangential transition connection in a curve, so that the connection part is in an arc-shaped chamfered surface, avoiding insufficient liquid injection or air bubbles being trapped in the transition position when injecting liquid.

[0132] Exemplarily, the first layer 11 can be formed by injection molding or machining. When injection molding, the accommodating groove 113 is processed, or the accommodating groove 113 is machined on the first layer 11, such as by laser co-cutting. At this time, since the depth of the accommodating groove 113 is related to the thickness of the accommodating cavity 13, the thickness of the first layer 11 can be determined according to the liquid layer thickness of the accommodating cavity 13, and the thickness of the first layer 11 can be approximately the same as the liquid layer thickness.

[0133] Optionally, the thickness of the flexible film is less than 0.2 mm. Further, the thickness of the flexible film is less than 0.1 mm or less than 0.05 mm, which is conducive to the rapid conduction of the temperatures of the heater and the cooler to the reaction sample.

[0134] Optionally, the first layer 11 and / or the second layer 12 can be made of a transparent material, facilitating fluorescence detection through the transparent first layer 11 and / or the second layer 12.

[0135] The first layer 11 and / or the second layer 12 can be a polycarbonate film, a polypropylene film or a polyimide film. These materials are not easily reactive with the reaction sample, thus not affecting the fluorescence detection result.

[0136] To further accelerate the heat conduction of the first layer 11 or the second layer 12, optionally, the first layer 11 or the second layer 12 is an aluminum film. In another embodiment, the first layer 11 or the second layer 12 includes an aluminum film and an isolation film, and the isolation film is connected to the inner side of the aluminum film. The isolation film can be a polycarbonate film, a polypropylene film or a polyimide film, etc., to reduce the influence of the aluminum film on the detection result.

[0137] Optionally, the accommodating cavity 13 is in a flat structure. It can be understood that the flat structure means that the dimension of the accommodating cavity 13 in the direction perpendicular to its thickness direction (the direction in which the first layer 11 and the second layer 12 are arranged is the thickness direction of the carrier) is greater than the dimension in its thickness direction. As an example, the ratio of the dimension of the accommodating cavity 13 in the direction perpendicular to its thickness direction to the dimension in its thickness direction is greater than 5:1, such as the ratio of dimensions being 50:1 to 100:1.

[0138] For example, the receiving cavity 13 is generally a cuboid, and the ratio of its length to its thickness can be greater than 5:1, such as a size ratio of 50:1 to 100:1. As an example, the size ratio is 90:1. The thickness of the receiving cavity 13 can be 0.1-1.0 mm, and its width and length are approximately 10 mm and 20 mm, respectively. As an example, the receiving cavity 13 can also be a cylindrical structure with a diameter to thickness ratio greater than 5:1, such as a thickness of 0.1-1.0 mm and a diameter of 5-20 mm. Of course, the cross-section of the receiving cavity 13 can be polygonal or elliptical, etc.

[0139] The reaction sample within the flat-structured cavity 13 is thin, and the distance between the center of the reaction sample and the liquid surface is very small. This allows the temperature of the reaction sample to reach uniformity in a very short time. Furthermore, the flat structure allows for a large contact area between the reaction sample and the heater or cooler, resulting in high heat transfer efficiency and significantly improved heating / cooling rates and detection efficiency. In contrast, the inner diameter of tubular carriers is relatively large compared to the flat-structured cavity 13. This results in a much greater distance between the center of the reaction sample and the liquid surface, requiring a longer time for the temperature to reach uniformity. Consequently, the heating / cooling rates are slower, leading to lower detection efficiency.

[0140] like Figure 1a As shown in Figure 5, the main body 1 provided in this embodiment has a sample inlet 14 communicating with the receiving cavity 13, thereby facilitating the addition of samples to the receiving cavity 13 through the sample inlet 14. The main body 1 also has an exhaust port 15 communicating with the receiving cavity 13. Therefore, while adding samples through the sample inlet 14, the air in the receiving cavity 13 can be discharged in a timely manner through the exhaust port 15, preventing the formation of bubbles in the reaction sample, thereby avoiding affecting fluorescence detection and improving the temperature uniformity of the reaction sample.

[0141] like Figure 1aAs shown in Figure 5, the reaction sample enters the receiving cavity 13 through the first end 131 of the receiving cavity 13 via the inlet 14. Air in the receiving cavity 13 enters the exhaust port 15 through the second end 132 of the receiving cavity 13. The first end 131 and the second end 132 are positioned opposite each other. The reaction sample enters the receiving cavity 13 from the first end 131 and then gradually fills the receiving cavity 13 towards the side where the second end 132 is located. Air in the receiving cavity 13 can enter the exhaust port 15 from the second end 132 until the receiving cavity 13 is completely filled with the reaction sample. That is, the exhaust port 15 will not be closed by the reaction sample before the entire reaction sample is filled, and can always exhaust air, thereby ensuring that the air in the receiving cavity 13 is completely exhausted, avoiding the inability of air to be discharged and affecting the smoothness of liquid injection, and avoiding the formation of bubbles affecting fluorescence detection. Optionally, when filling the reaction sample, the first end 131 is located below the second end 132. That is, when filling the reaction sample, the liquid level of the reaction sample gradually rises, and the air with a lower density (relative to the reaction sample) can always be located above the reaction sample, thereby being discharged from the exhaust port 15.

[0142] like Figures 1b-1c As shown, the exhaust port 15 and the sample inlet 14 can both be located on the side of the receiving cavity 13 near the second end 132. In other optional embodiments, the exhaust port 15 and the sample inlet 14 can also both be located on the side of the receiving cavity 13 near the first end 131, that is, the exhaust port 15 and the sample inlet 14 are located on one side of the receiving cavity 13. Such a sealing structure (described in detail below) can seal the exhaust port 15 and the sample inlet 14 at the same time, simplifying the carrier structure, and the exhaust port 15 and the sample inlet 14 can be sealed at the same time in one operation, which is convenient to operate.

[0143] like Figure 3 As shown, in another embodiment, the first end 131 and the second end 132 are located between the vent port 15 and the injection port 14, with the injection port 14 positioned closer to the first end 131 and the vent port 15 positioned closer to the second end 132. This allows the pipette to be inserted into the injection port 14 without interference from the vent port 15, facilitating pipette positioning and improving injection efficiency.

[0144] like Figure 4 As shown, in another embodiment, the inlet 14 is disposed opposite to the first end 131 of the receiving cavity 13, and the exhaust port 15 is disposed opposite to the second end 132 of the receiving cavity 13. In other words, the inlet 14 opened on the first layer 11 or the second layer 12 is directly opposite to the first end 131, so that the inlet 14 is directly connected to the receiving cavity 13, and the exhaust port 15 opened on the first layer 11 or the second layer 12 is directly opposite to the second end 132, so that the exhaust port 15 is directly connected to the receiving cavity 13.

[0145] Optionally, regardless of their positions relative to the receiving cavity 13, the exhaust port 15 and the sample inlet 14 can both be located in the first layer 11, or both can be located in the second layer 12 (e.g., Figure 1b As shown), only one layer needs to be processed for openings (vent 15 and injection port 14). Injection port 14 is opened in one of the first layer 11 and the second layer 12, and vent 15 is opened in the other of the first layer 11 and the second layer 12. That is, vent 15 and injection port 14 are located on opposite sides of the carrier. In other words, vent 15 is located on one side of the carrier, and injection port 14 is located on the other side of the carrier. In this way, vent 15 and injection port 14 are set separately. When the pipette is inserted into injection port 14, it is not affected by vent 15, which facilitates pipette positioning and improves injection efficiency.

[0146] like Figures 2-3 As shown, when the inlet 14 and / or the outlet 15 are not directly connected to the receiving cavity 13, the inlet 14 and the receiving cavity 13 may optionally be connected through the inlet channel 16 to set the position of the inlet 14 according to actual needs. The outlet 15 and the receiving cavity 13 may also be connected through the outlet channel 17 to set the position of the outlet 15 according to actual needs.

[0147] Optionally, one end of the injection channel 16 is connected to the first end 131 and the other end is connected to the injection port 14, so that the reaction sample enters the containment cavity 13 from the first end 131 and then gradually fills the containment cavity 13.

[0148] Optionally, one end of the exhaust passage 17 is connected to the second end 132, and the other end is connected to the exhaust port 15, so that air enters the exhaust port 15 from the second end 132 of the receiving cavity 13 through the exhaust passage 17.

[0149] When the deformability of the first layer 11 is lower than that of the second layer 12, a flow channel 114 and / or an exhaust channel 115 can be formed on the first layer. Optionally, the aforementioned receiving groove 113 can be formed on the first layer 11. The second layer 12 and the exhaust channel 115 form the exhaust channel 17, and / or the second layer 12 and the flow channel 114 form the sample inlet channel 16. It is understood that the flow channel 114 and / or the exhaust channel 115 are connected to the receiving groove 113 or the recess 112, so that the exhaust channel 17 and / or the sample inlet channel 16 are connected to the receiving cavity 13. The flow channel 114 and the exhaust channel 115 have a certain depth, thus ensuring that the cross-sectional area of ​​the sample inlet channel 16 and the exhaust channel 17 is large, which is more conducive to the flow of reaction samples and the exhaust.

[0150] In other alternative embodiments, such as Figure 3As shown, two spaced first connecting strips 161 are formed between the first layer 11 and the second layer 12, and the sample inlet channel 16 is formed between the two first connecting strips 161. The sample inlet channel 16 is formed in a simple way, such as by connecting the first layer 11 and the second layer 12 to form the first connecting strips 161 through laser welding, bonding, or low-temperature bonding. And / or, two spaced second connecting strips 171 are formed between the first layer 11 and the second layer 12, and the exhaust channel 17 is formed between the two second connecting strips 171. The exhaust channel 17 is formed in a simple way, such as by connecting the first layer 11 and the second layer 12 to form the second connecting strips 171 through laser welding, bonding, or low-temperature bonding.

[0151] Optionally, the first layer 11 and the second layer 12 are connected by laser welding, bonding or low-temperature bonding. For example, laser welding provides reliable connection strength, simple connection method and strong operability.

[0152] For example, when processing the carrier in this embodiment, the first layer 11 and the second layer 12 are connected by laser welding, bonding or low temperature bonding. The connection position is the outer contour of the carrier, the receiving cavity 13, the exhaust port 15, the sample inlet 14, the exhaust channel 17, the sample inlet channel 16 and the connecting part 111. The membrane material at the sample inlet 14 and the exhaust port of the first layer 11 and / or the second layer 12 is removed by laser or other machining methods.

[0153] like Figure 1b and Figures 5a-5c As shown, the carrier also includes a sealing structure to restrict the flow of reaction sample from the inlet 14 and / or the vent 15. Specifically, after liquid injection is completed, the inlet 14 and / or the vent 15 can be sealed by the sealing structure to prevent contamination such as aerosols.

[0154] Optionally, the sealing structure may include a first sealing component (the first sealing component is the same as the first sealing component 5 in Embodiment 3 below) capable of restricting the outflow of the reaction sample from the vent 15; and / or the sealing structure may include a second sealing component (the second sealing component is the same as the second sealing component 4 in Embodiment 3 below) capable of sealing the inlet 14. When the distance between the vent 15 and the inlet 14 is large, or when the vent 15 and the inlet 14 are located on opposite sides of the carrier, a first sealing component and a second sealing component may be provided to connect to the vent 15 and the inlet 14, respectively.

[0155] like Figure 1bAs shown, in another embodiment, the sealing structure may include a third sealing component 3 capable of sealing the exhaust port 15 and the injection port 14. For example, when the distance between the exhaust port 15 and the injection port 14 is small and the exhaust port 15 and the injection port 14 are located on the same side of the carrier, the third sealing component 3 may be provided to seal both the exhaust port 15 and the injection port 14 simultaneously.

[0156] like Figures 5a-5c As shown, optionally, the sealing structure (the sealing structure can be at least one of the third sealing component 3 or the first sealing component 5 and the second sealing component 4) is capable of compressing the air in the carrier and causing the first layer 11 and / or the second layer 12 to protrude (it is understood that whether the first layer 11 protrudes depends on the specific circumstances. When the thickness of the first layer 11 is large, the force applied to the first layer 11 by the reaction sample is insufficient to deform the first layer 11, and the first layer 11 will not protrude; conversely, when the thickness of the first layer 11 is small, the force applied to the first layer 11 by the reaction sample is sufficient to deform the first layer 11, which will cause the first layer 11 to protrude). When the container 13 contains a reaction sample, the pressure inside the carrier is the same as the external pressure. When the inlet 14 and / or outlet 15 are sealed by the sealing structure, the sealing structure can create internal pressure in the reagent chamber, compressing the reaction sample. The reaction sample compresses the first layer 11 and the second layer 12, causing the first layer 11 and / or the second layer 12 to bulge outward. The flexible film is easy to bulge outward under the action of internal pressure, thereby preventing the first layer 11 and / or the second layer 12 from collapsing and affecting fluorescence reading. In addition, the connecting part 111 can also control the thickness of the container 13, that is, prevent the second layer 12 from collapsing and prevent the container 13 from bulging outward too much, thereby improving the stability of the test.

[0157] like Figure 1b , Figures 5a-5c As shown, specifically, the sealing structure includes an insert 32 and a seal 31. The insert 32 is sealed to the main body 1 and covers the exhaust port 15 and / or the sample inlet 14. The insert 32 has a sealing cavity 321 communicating with the exhaust port 15 and / or the sample inlet 14. Specifically, when the sealing structure seals the exhaust port 15, such as when the sealing structure includes a first sealing component 5, the sealing cavity 321 covers the exhaust port 15; when the sealing structure seals the sample inlet 14, such as when the sealing structure includes a second sealing component 4, the sealing cavity 321 covers the sample inlet 14; when the sealing structure seals both the sample inlet 14 and the exhaust port 15, such as when the sealing structure includes a third sealing component 3, the sealing cavity 321 covers both the sample inlet 14 and the exhaust port 15. During injection, the injection gun is inserted into the sealing cavity 321 and injected into the sample inlet 14. The specific structure of the sealing structure can be the same as the sealing structure in Embodiment 1, and will not be described again here.

[0158] At least a portion of the seal 31 is capable of sealing the inserted sealing cavity 321 to at least compress the air within the sealing cavity 321 and to allow the first layer 11 and / or the second layer 12 to protrude outwards. The seal 31 not only seals the exhaust port 25 and / or the sample inlet 24, but also allows the first layer 11 and the second layer 12 to protrude outwards, preventing the first layer 11 and the second layer 12 from collapsing and ensuring detection accuracy.

[0159] Furthermore, the seal 31 can seal the insertion into the sealing cavity 321 to compress the air within the sealing cavity 321 and the receiving cavity 13. Optionally, the seal 31 is interference-fitted with the sealing cavity 321 to seal the sealing cavity 321.

[0160] At least part of the seal 31 is inserted into the sealing cavity 321. On the one hand, since the seal 31 occupies the space inside the carrier (specifically the sealing cavity 321 of the inserted member 32), the gas inside the carrier is compressed. For example, when the receiving cavity 13 is filled with the reaction sample, the gas inside the sealing cavity 321 is compressed, or when the receiving cavity 13 carries the reaction sample but the reaction sample does not fill the receiving cavity 13, the gas inside the sealing cavity 321 and the receiving cavity 13 is compressed, making the pressure inside the carrier greater than the external pressure, thereby causing the first layer 11 and / or the second layer 12 to bulge outward, thus avoiding the collapse of the first layer 11 and the second layer 12 and affecting the fluorescence reading.

[0161] For example, when processing the carrier in this embodiment, the first layer 11 and the second layer 12 are connected by laser welding, bonding or low temperature bonding. The connection positions are the outer contours of the carrier, the receiving cavity 13, the exhaust port 15, the sample inlet 14, the exhaust channel 17, the sample inlet channel 16 and the connecting part 111. Then, the membrane material at the sample inlet 14 and the exhaust port of the first layer 11 and / or the second layer 12 is removed by iso-machining. Finally, the insert 32 is laser welded, bonded or low temperature bonded to the sample inlet 14 and / or the exhaust port 15.

[0162] Example 2

[0163] like Figures 6A-6C As shown, this embodiment provides a carrier including a main body 2. The main body 2 includes a first layer 21 and a second layer 22 disposed opposite to each other. Both the first layer 21 and the second layer 22 are flexible films. The first layer 21 and the second layer 22 are connected to each other at opposite portions and form a receiving cavity 23 in the unconnected portions. At least one connecting portion 211 is formed in the receiving cavity 23. The connecting portion 211 connects the first layer 21 and the second layer 22 to reduce the deformation of the receiving cavity 23.

[0164] It is understood that since the connecting part 211 is formed in the receiving cavity 23 and connects the first layer 21 and the second layer 22, in this embodiment, the portion of the receiving cavity 23 other than the portion 211 can accommodate the reaction sample, while the position of the receiving cavity 23 where the connecting part 211 is formed cannot accommodate the reaction sample.

[0165] In this embodiment, both the first layer 21 and the second layer 22 are flexible films, and the carrier is formed by two flexible films, resulting in a simple structure and low cost. Both the first layer 21 and the second layer 22, which form the receiving cavity 23, are flexible films and do not have heating functions, further reducing the cost of the carrier. Furthermore, since both the first layer 21 and the second layer 22 are flexible films, their thinness allows for rapid heat transfer to the reaction sample during heating and cooling, enabling rapid temperature adjustment and shortening the PCR reaction time. Additionally, the small overall volume of the flexible film results in a low heat capacity, minimizing heat loss during heating and cooling of the reaction sample and reducing the energy consumption of the heater and cooler. On the other hand, because both the first layer 21 and the second layer 22 are flexible films, roll-to-roll packaging can be used, reducing the mold development costs of traditional injection molding processes and significantly lowering material and processing costs.

[0166] In this embodiment, the first layer 21 is provided with an outwardly protruding receiving groove 213. The receiving groove 213 includes a connecting groove 2131 and at least two sub-grooves 2132, and two adjacent sub-grooves 2132 are connected through the connecting groove 2131. Figure 6C As shown, protrusions extending from the two sidewalls of the receiving groove 213 in opposite directions form connecting portions 211, and the two connecting portions 211 constitute the communicating groove 2131. In a top view, the cross-sectional area of ​​the communicating groove 2131 is smaller than the cross-sectional area of ​​the sub-groove 2132. It is understood that "outer" refers to the side of the first layer 21 away from the receiving cavity 23 or away from the second layer 22. In other embodiments, the connecting portions 211 may also extend only from one sidewall of the receiving groove 213. In yet another embodiment, such as Figure 7 and 8 As shown, the second carrier in this embodiment is as follows: Figure 8 As shown, protrusions extending from both sidewalls of the receiving groove 213 form connecting portions 211. Multiple connecting portions 211 are arranged alternately along the sidewalls of the receiving groove 213, and the space between the connecting portions 211 and the other sidewall of the receiving groove 213 forms a communicating groove 2131. In fact, the arrangement of the connecting portions 211 in this embodiment is similar to that in Embodiment 1. Figures 1b to 1c As shown, it will not be elaborated further here.

[0167] From the top view, the outer edge of the connecting part 211 is curved. Liquid flow exhibits a wall-adhering effect; when the outer edge of the connecting part 211 is curved, it facilitates better flow of the reaction liquid within the receiving cavity 23 and prevents the formation of bubbles in the reaction sample as it flows through the connecting part 211. In contrast, if the outer edge of the connecting part 211 were a right angle, the wall-adhering effect would be poor when the reaction liquid reaches the right angle position, and bubbles would form due to impact during flow. Furthermore, the connection between the connecting part 211 and the side wall of the receiving cavity 23 is curved, with a tangential curve transition, resulting in a rounded, chamfered curved surface. This prevents insufficient liquid injection or bubbles trapped in the transition position during liquid injection.

[0168] The sub-groove 2132 can be of a regular shape or an irregular shape, and the shapes of the sub-groove 2132 can be the same or different. The first layer 21 between two adjacent sub-grooves 2132 forms the connecting part 211, so that it can be connected to the second layer 22, thereby preventing the receiving cavity 23 from bulging outward or collapsing uncontrollably after the addition of reaction samples.

[0169] like Figures 6A-6C As shown, specifically, when both the first layer 21 and the second layer 22 are flexible films, the flow channel 214 and / or the exhaust channel 215 are both connected to the recess 212. For example, during the vacuum forming of the first layer 21, the flow channel 214 and / or the exhaust channel 215 are formed. At this time, the outer side (the side facing away from the second layer 22) of the first layer 21 at the position corresponding to the flow channel 214 and / or the exhaust channel 215 protrudes outward (e.g., Figure 6C As shown), the flow channel groove 214 and / or exhaust channel groove 215 are recessed relative to the second layer 22 on the side facing the second layer 22.

[0170] Optionally, the first layer 21 and the second layer 22 are connected by laser welding, bonding or low-temperature bonding. For example, laser welding provides reliable connection strength, simple connection method and strong operability.

[0171] like Figure 9 As shown, in the third carrier provided in this embodiment, the first layer 21 and the second layer 22 are connected and form a receiving cavity 23. A connecting part 211 is provided in the receiving cavity 23. The connecting part 211 is a columnar structure, with one end connected to the first layer 21 and the other end connected to the second layer 22. Optionally, the connecting part 211 can be integrally formed with the first layer 21 or the second layer 22.

[0172] Optionally, the thickness of the flexible film is less than 0.2 mm, and even further, the thickness of the flexible film is less than 0.1 mm or less than 0.05 mm, which facilitates the rapid transfer of temperature from the heater and cooler to the reaction sample.

[0173] Optionally, the first layer 21 and / or the second layer 22 may be made of a transparent material to facilitate fluorescence detection through the transparent first layer 21 and / or second layer 22.

[0174] The first layer 21 and / or the second layer 22 can be a polycarbonate film, a polypropylene film, or a polyimide film. These materials are not easily reacted with the reaction sample, so they will not affect the fluorescence detection results.

[0175] Optionally, the sealing structure may include a first sealing component (the first sealing component is the same as the first sealing component 5 in Embodiment 3 below) capable of restricting the outflow of the reaction sample from the vent 25; and / or the sealing structure may include a second sealing component (the second sealing component is the same as the second sealing component 4 in Embodiment 3 below) capable of sealing the inlet 24. When the distance between the vent 25 and the inlet 24 is large, or when the vent 25 and the inlet 24 are located on opposite sides of the carrier, a first sealing component and a second sealing component may be provided to connect to the vent 25 and the inlet 24 respectively. When the distance between the vent 25 and the inlet 24 is small, the third sealing component 3 in Embodiment 1 may be used to seal the vent 25 and the inlet 24; the implementation method is the same as in Embodiment 1 and will not be repeated here.

[0176] When the second layer 22 is connected to the first layer 21, the exhaust channel 215 and the second layer 22 form an exhaust channel 27, and the flow channel 214 and the second layer 22 form a sample inlet channel 26. The specific structure is the same as that in Embodiment 1, and will not be described again here.

[0177] Example 3

[0178] like Figures 10-20 As shown, the carrier provided in this embodiment includes a main body 6, which includes a third layer 68 and a first layer 61 and a second layer 62 disposed opposite to each other. The first layer 61 and the second layer 62 are both flexible films. The deformability of the third layer 68 is lower than that of the first layer 61 and the second layer 62. The first layer 61 and the second layer 62 are connected to each other on both sides of the thickness direction of the third layer 68. The first layer 61, the second layer 62, and the third layer 68 form the receiving cavity 63. At least one connecting portion 686 is formed within the receiving cavity 63, connecting the first layer 61 and the second layer 62 to reduce the deformation of the receiving cavity 63. The connecting portion 686 and the third layer 68 are either an integral structure or separate structures.

[0179] Understandably, low deformability means that under the same force, the third layer 68 is less prone to deformation than the first layer 61 and the second layer 62. Specifically, the deformability of the third layer 68 can be made lower than that of the first layer 61 and the second layer 62 through thickness design or material selection. For example, the thickness of the third layer 68 is greater than the thickness of the first layer 61 and the second layer 62, and / or the material flexibility and / or stiffness of the third layer 68 are greater than those of the first layer 61 and the second layer 62.

[0180] The carrier provided in this embodiment is formed by three layers, resulting in a simple structure and low cost. The first layer 61, the second layer 62, and the third layer 68 do not have heating functions, further reducing the cost of the carrier. The deformability of the third layer 68 is lower than that of the first layer 61 and the second layer 62, thereby increasing the overall strength of the carrier and preventing deformation.

[0181] Both the first layer 61 and the second layer 62 are flexible films. The flexible film has a small thickness, and when the heater and cooler are used as heating carriers, heat can be quickly transferred to the reaction sample through the flexible film. In addition, the flexible film has a small thickness and a small overall volume, resulting in a small heat capacity. Therefore, the heat loss generated at the flexible film is small when heating and cooling the reaction sample, and the energy consumption of the heater and cooler is small.

[0182] It is understood that since the connecting part 686 is formed in the receiving cavity 63 and connects the first layer 61 and the second layer 62, in this embodiment, the portion of the receiving cavity 63 other than the portion 686 can accommodate the reaction sample, while the position of the receiving cavity 63 where the connecting part 686 is formed cannot accommodate the reaction sample.

[0183] Because the cross-sectional area of ​​the receiving cavity 63 parallel to the thickness direction of the carrier is large, and the first layer 61 and the second layer 62 are flexible films, the receiving cavity 63 is prone to deformation after bearing the reaction sample. This makes it difficult to control the thickness of the receiving cavity 63, and the thickness is unstable, resulting in poor detection stability. In this embodiment, by forming at least one connecting portion 686 in the receiving cavity 63, the first layer 61 and the second layer 62 are connected through the connecting portion 686. Therefore, the connecting portion 686 can limit the bulging or collapse of the surrounding first layer 61 and second layer 62, thereby reducing the deformation of the receiving cavity 63 and controlling its thickness. The number of connecting portions 686 can be set according to the size of the receiving cavity 63, thereby controlling the overall thickness of the receiving cavity 63, making the thickness of the receiving cavity 63 easy to control and stable, and improving the stability of the detection.

[0184] In addition, the connecting part 686 occupies part of the space of the receiving cavity 63, which effectively reduces the volume of the non-detection area in the receiving cavity 63, reduces the amount of reaction sample used, and saves costs.

[0185] like Figure 12 and Figure 13 As shown, the third layer 68 has a through-groove 681 extending through its thickness direction. The through-groove 681, the first layer 61, and the second layer 62 form a cavity 63 for accommodating the reaction sample. A connecting portion 686 extends from one sidewall of the through-groove 681 to the other sidewall, connecting the first layer 61 and the second layer 62. As shown in the figure, in this embodiment, the connecting portion 686 and the third layer 68 are integrally formed, extending integrally from one sidewall of the through-groove 681 to the other sidewall. In other embodiments, the connecting portion 686 can also be formed by applying adhesive to both sides of the sidewall of the through-groove 681.

[0186] The receiving slot 681 includes a connecting slot 6811 and at least two sub-slots 6812, with two adjacent sub-slots 6812 connected through the connecting slot 6811.

[0187] Reference Figure 12 As shown, in a preferred embodiment, both sidewalls of the receiving channel 681 are provided with connecting portions 686, which are arranged opposite to each other. The space between the connecting portions 686 of the two sidewalls forms a connecting channel 6811. The connecting channel 6811 in this embodiment makes it easier for the reaction sample to flow between the two sub-channels 6812.

[0188] Reference Figure 13 As shown, in another preferred embodiment, both sidewalls of the receiving channel 681 are provided with connecting portions 686, which are staggered. The space between the connecting portions 686 and the other sidewall of the receiving channel 681 forms a communicating channel 6811. In this embodiment, the communicating channel 6811 and the sub-channel 6812 form an "S"-shaped structure in the top view, which can better discharge air from the receiving cavity 63.

[0189] In another preferred embodiment, a connecting portion 686 is provided on one side wall of the receiving channel 681, and the space between the connecting portion 686 and the other side wall of the receiving channel 681 constitutes the communicating channel 6811.

[0190] The side wall of the connecting through groove 6811 forms the connecting portion 686. In the top view direction, the cross-sectional area of the connecting through groove is smaller than the cross-sectional area of the sub-through groove (or the cross-section is the section of the connecting through groove or the sub-through groove in the thickness direction of the third layer 68). That is, the space surrounded by the connecting through groove, the sub-through groove, the first layer 61 and the second layer 62 is used to accommodate the reaction sample. The connecting portion 686 formed by the side wall of the connecting through groove makes the cross-sectional area of the connecting through groove smaller than the cross-sectional area change of the sub-through groove. The cross-sectional area change of the connecting through groove and the sub-through groove indicates the cross-sectional area change of the space for accommodating the reaction sample. When the cross-sectional area becomes smaller, it can limit the excessive protrusion or collapse of the accommodation cavity 63.

[0191] The thickness of the connecting portion 686 can be the same as the thickness at the position where the accommodation through groove 681 is not opened in the third layer 68, so that the surface of the connecting portion 686 is flush with the surface at the position where the accommodation through groove 681 is not opened in the third layer 68, so that the accommodation cavity 63 is not easily collapsed.

[0192] As Figure 12 and Figure 13 shown, optionally, the surfaces of the connecting portion 686 facing the accommodation through groove 681 are all arc surfaces. There is a wall adhesion effect in the flow of liquid. When the outer edge surface of the connecting portion 686 is an arc surface, on the one hand, it is convenient for the reaction liquid to have better fluidity when flowing in the accommodation cavity 63, and on the other hand, it can avoid the formation of bubbles when the reaction sample flows through the connecting portion 686. At the same time, it can also avoid the formation of trapped air at the above-mentioned arc surface when injecting the first layer 61. In contrast, if the outer edge surface of the connecting portion 686 is a right-angle surface, the wall adhesion effect of the reaction liquid when flowing to the right-angle position is poor, and bubbles will be formed due to the impact when the reaction liquid flows. The connection between the connecting portion 686 and the side wall of the accommodation cavity 63 is an arc surface. As Figure 13 shown, the connecting portion 686 is in a "ji" shape, and its protruding end is in a circular arc shape. The connection between the connecting portion 686 and the side wall of the accommodation cavity 63 is connected by a curve tangent transition, so that the connection is in a circular arc chamfer arc surface, avoiding insufficient liquid injection or trapped air at the transition position when injecting liquid. [[ID=1x]]

[0193] The number of the connecting portions 686 is at least two. The more the number of the connecting portions 686, the easier it is to control the liquid layer thickness in the accommodation cavity 63. However, if the number of the connecting portions 686 is too large, it may cause difficulties in liquid injection. Therefore, the number of the connecting portions 686 can be determined according to the specific situation. The shapes of the connecting portions 686 can be the same or different, and can be determined according to needs or processing convenience. Optionally, a plurality of connecting portions 686 are arranged along the length direction of the accommodation cavity 63, so that the thickness distribution of the accommodation cavity 63 is uniform.

[0194] Optionally, the third layer 68 is made of polycarbonate, polypropylene, polyimide or polyethylene, which are materials that do not readily react with the reaction sample and thus do not affect the fluorescence detection results.

[0195] Optionally, the thickness of the third layer 68 is less than 0.3 mm, more preferably, the thickness of the third layer 68 is less than 0.2 mm. For example, the thickness of the third layer 68 is determined according to the thickness of the reaction sample, and the thickness of the third layer 68 can be approximately the same as the thickness of the liquid layer.

[0196] The third layer 68 has a smaller thickness and overall volume, resulting in a smaller heat capacity. This leads to less heat loss at the third layer 68 during heating and cooling of the reaction sample, thus reducing the energy consumption of the heater and cooler. Simultaneously, the smaller thickness of the third layer 68 results in a thinner cavity 63, facilitating the formation of a thin reagent layer and enabling the reaction sample to quickly reach the predetermined temperature and achieve uniform temperature.

[0197] Optionally, the thickness of the first layer 61 and / or the second layer 62 is less than 0.2 mm, and further, the thickness of the first layer 61 and / or the second layer 62 is less than 0.1 mm or less than 0.05 mm, which facilitates the rapid transfer of heater and cooler temperature to the reaction sample.

[0198] The thinness of the third layer 68, the first layer 61, and the second layer 62 allows for rapid heating and cooling of the reaction samples, shortening the PCR reaction time. Furthermore, because the third layer 68, the first layer 61, and the second layer 62 are all thin layers, they can be packaged using roll-to-roll manufacturing processes, reducing the mold development costs of traditional injection molding and significantly lowering material and processing costs.

[0199] Optionally, the first layer 61 and / or the second layer 62 may be made of a transparent material to facilitate fluorescence detection through the transparent first layer 61 and / or the second layer 62. The third layer 68 may also be made of a transparent material.

[0200] The first layer 61 and / or the second layer 62 can be a polycarbonate film, a polypropylene film, or a polyimide film. These materials do not readily react with the reaction sample, thus not affecting the fluorescence detection results.

[0201] To further accelerate heat conduction in the first layer 61 or the second layer 62, optionally, the first layer 61 or the second layer 62 is an aluminum film. In another embodiment, the first layer 61 or the second layer 62 includes an aluminum film and a separator film, the separator film being connected to the inner side of the aluminum film. The separator film can be a polycarbonate film, a polypropylene film, or a polyimide film, etc., to reduce the influence of the aluminum film on the detection results.

[0202] Optionally, the first layer 61 and the third layer 68, as well as the second layer 62 and the third layer 68, are connected by laser welding, bonding, or cryogenic bonding. For example, laser welding provides reliable connection strength, a simple connection method, and high operability.

[0203] The third layer 68 can be formed by machining or injection molding. Correspondingly, the receiving groove 681 can be formed by machining or injection molding, such as by laser cutting. Specifically, the third layer 68 is laser-cut to form the receiving groove 681 and the connecting part 686. Then, the first layer 61 is welded to the third layer 68 and the second layer 62 is welded to the third layer 68 using a laser. The welding positions are around the carrier and the outer contour of the receiving groove 681 and the connecting part 686 formed in the previous step.

[0204] like Figure 10 , Figures 14 to 16 As shown, optionally, the receiving cavity 63 has a flat structure. It can be understood that a flat structure means that the dimension of the receiving cavity 63 perpendicular to its thickness direction (the direction in which the first layer 61 and the second layer 62 are set is the thickness direction of the carrier) is greater than its dimension in the thickness direction. For example, the ratio of the dimension of the receiving cavity 63 perpendicular to its thickness direction to its dimension in the thickness direction is greater than 5:1, such as a ratio of 50:1 to 100:1.

[0205] For example, the receiving cavity 63 is generally a cuboid, and the ratio of its length to its thickness can be greater than 5:1, such as a size ratio of 50:1 to 100:1. As an example, the thickness of the receiving cavity 63 can be 0.1-0.5 mm, and the width and length of the receiving cavity 23 are approximately 10 mm and 20 mm, respectively. As an example, the receiving cavity 23 can also be a cylindrical structure with a diameter to thickness ratio greater than 5:1, such as a thickness of 0.1-0.5 mm and a diameter of 5-20 mm. Of course, the cross-section of the receiving cavity 63 can be polygonal or elliptical, etc.

[0206] The reaction sample within the flat-structured cavity 63 is thin, and the distance between the center of the reaction sample and the liquid surface is very small. This allows the temperature of the reaction sample to reach uniformity in a very short time. Furthermore, the flat structure allows for a large contact area between the reaction sample and the heater or cooler, resulting in high heat transfer efficiency and significantly improved heating / cooling rates and detection efficiency. In contrast, the inner diameter of the tubular carrier is relatively large compared to the flat-structured cavity 63, resulting in a much greater distance between the center of the reaction sample and the liquid surface. This leads to a longer time required for the temperature of the reaction sample to reach uniformity, slower heating / cooling rates, and lower detection efficiency.

[0207] like Figures 11-18As shown, the main body 6 may also have an exhaust port 65 communicating with the receiving cavity 63. Therefore, while the sample is added through the sample inlet 64, the air in the receiving cavity 63 can be discharged in time through the exhaust port 65, preventing the formation of bubbles in the reaction sample, thereby avoiding affecting the fluorescence detection and improving the temperature uniformity of the reaction sample.

[0208] The main body 6 may also have a sample inlet 64 that communicates with the receiving cavity 63, so that it is convenient to add samples to the receiving cavity 63 through the sample inlet 64.

[0209] like Figures 14-16 As shown, the reaction sample enters the receiving cavity 63 through the first end 631 of the receiving cavity 63 via the sample inlet 64, and the air in the receiving cavity 63 enters the exhaust port 65 through the second end 632 of the receiving cavity 63. The first end 631 and the second end 632 are arranged opposite to each other. Exemplarily, a plurality of connecting parts 686 are arranged along the direction in which the first end 631 and the second end 632 are located.

[0210] The reaction sample enters the receiving cavity 63 from the first end 631 and then gradually fills the receiving cavity 63 to the side where the second end 632 is located. Air in the receiving cavity 63 can enter the exhaust port 65 from the second end 632 until the reaction sample fills the receiving cavity 63. That is, before the reaction sample fills the entire receiving cavity 63, the exhaust port 65 will not be blocked by the reaction sample and can always exhaust air, ensuring that the air in the receiving cavity 63 is completely exhausted, avoiding the inability of air to be discharged and affecting the liquid inlet, and avoiding the formation of bubbles that affect fluorescence detection.

[0211] Optionally, when filling the reaction sample, the first end 631 is located below the second end 632. That is, when filling the reaction sample, the liquid level of the reaction sample gradually rises, and the air with a lower density (relative to the reaction sample) can always be located above the reaction sample, thereby being discharged from the exhaust port 65.

[0212] like Figure 14 As shown, the exhaust port 65 and the sample inlet 64 can both be located on the side of the receiving cavity 63 near the second end 632. In other optional embodiments, the exhaust port 65 and the sample inlet 64 can also both be located on the side of the receiving cavity 63 near the first end 631, that is, the exhaust port 65 and the sample inlet 64 are located on one side of the receiving cavity 63. Such a sealing structure (described in detail below) can seal the exhaust port 65 and the sample inlet 64 at the same time, simplifying the carrier structure, and the exhaust port 65 and the sample inlet 64 can be sealed at the same time in one operation, which is convenient to operate.

[0213] like Figure 15As shown, in another embodiment, the first end 631 and the second end 632 are located between the vent port 65 and the injection port 64, with the injection port 64 positioned closer to the first end 631 and the vent port 65 positioned closer to the second end 632. This allows the pipette to be inserted into the injection port 64 without interference from the vent port 65, facilitating pipette positioning and improving injection efficiency.

[0214] like Figure 16 and Figure 17 As shown, in another embodiment, the inlet 64 is disposed opposite to the first end 631 of the receiving cavity 63, and the exhaust port 65 is disposed opposite to the second end 632 of the receiving cavity 63. In other words, the inlet 64 opened on the first layer 61 or the second layer 62 is directly opposite to the first end 631, so that the inlet 64 is directly connected to the receiving cavity 63, and the exhaust port 65 opened on the first layer 61 or the second layer 62 is directly opposite to the second end 632, so that the exhaust port 65 is directly connected to the receiving cavity 63.

[0215] Optionally, regardless of their positions relative to the receiving cavity 63, the vent 65 and the inlet 64 can both be located in the first layer 61 (e.g., Figure 17 (as shown), or they can all be opened on the second floor, 62 (as shown). Figure 11 As shown), this only requires machining openings (vent 65 and inlet 64) on one layer; or as... Figure 18 As shown, the injection port 64 is located in one of the first layer 61 and the second layer 62, and the vent port 65 is located in the other of the first layer 61 and the second layer 62. That is, the vent port 65 and the injection port 64 are located on opposite sides of the carrier. In other words, the vent port 65 is located on one side of the carrier, and the injection port 64 is located on the other side of the carrier. In this way, the vent port 65 and the injection port 64 are set separately. When the pipette is inserted into the injection port 64, it can be unaffected by the vent port 65, which facilitates the positioning of the pipette and improves the injection efficiency.

[0216] like Figure 19 As shown, in another embodiment, both the inlet 64 and the outlet 65 are located on the side of the third layer 68. This eliminates the need to machine openings (outlet 65 and inlet 64) into the first layer 61 and the second layer 62, reducing the number of machining steps required for the first layer 61 and the second layer 62.

[0217] In other alternative embodiments, one of the inlet 64 and the outlet 65 may be located on the side of the third layer 68, and the other may be located in the first layer 61 or the second layer 62.

[0218] like Figure 20As shown, to form an exhaust port 65 located on the side of the third layer 68, an exhaust groove 682 is provided on the third layer 68. The exhaust groove 682 communicates with the receiving cavity 63 and extends to the side of the third layer 68. At least one of the first layer 61 and the second layer 62 forms the exhaust port 65 with the groove wall of the exhaust groove 682. Specifically, when the exhaust groove 682 penetrates the thickness direction of the third layer 68 (e.g.) Figure 20 The exhaust port 65 is formed by the wall of the exhaust groove 682, the first layer 61, and the second layer 62. When the exhaust groove 682 does not penetrate the thickness direction of the third layer 68, and the side of the exhaust groove 682 facing the first layer 61 penetrates the thickness direction of the third layer 68, the first layer 61 and the wall of the exhaust groove 682 form the exhaust port 65; when the exhaust groove 682 does not penetrate the thickness direction of the third layer 68, and the side of the exhaust groove 682 facing the second layer 62 penetrates the thickness direction of the third layer 68, the second layer 62 and the wall of the exhaust groove 682 form the exhaust port 65. The formation of the exhaust port 65 is simple and easy to process.

[0219] like Figure 20 As shown, in order to form a sample inlet 64 located on the side of the third layer 68, a sample inlet groove 683 may also be provided on the third layer 68. The sample inlet groove 683 communicates with the receiving cavity 63 and extends to the side of the third layer 68. At least one of the first layer 61 and the second layer 62, together with the groove wall of the sample inlet groove 683, forms the sample inlet 64. Specifically, when the sample inlet groove 683 penetrates the thickness direction of the third layer 68, the groove wall of the sample inlet groove 683, the first layer 61, and the second layer 62 form the sample inlet 64. When the injection groove 683 does not penetrate the thickness direction of the third layer 68, and the side of the injection groove 683 facing the first layer 61 penetrates the thickness direction of the third layer 68, the first layer 61 and the groove wall of the injection groove 683 form the injection port 64; when the injection groove 683 does not penetrate the thickness direction of the third layer 68, and the side of the injection groove 683 facing the second layer 62 penetrates the thickness direction of the third layer 68, the second layer 62 and the groove wall of the injection groove 683 form the injection port 64. The formation of the injection port 64 is simple and easy to process.

[0220] like Figures 14-15 As shown, when the inlet 64 and / or the outlet 65 are not directly connected to the receiving cavity 63, optionally, the inlet 64 and the receiving cavity 63 can be connected through the inlet channel 66 to set the position of the inlet 64 according to actual needs. The outlet 65 and the receiving cavity 63 can also be connected through the outlet channel 67 to set the position of the outlet 65 according to actual needs.

[0221] Optionally, one end of the injection channel 66 is connected to the first end 631, and the other end is connected to the injection port 64, so that the reaction sample enters the cavity 63 from the first end 631 and then gradually fills the cavity 63.

[0222] Optionally, one end of the exhaust passage 67 is connected to the second end 632, and the other end is connected to the exhaust port 65, so that air enters the exhaust port 65 from the second end 632 of the receiving cavity 63 through the exhaust passage 67.

[0223] like Figure 13 and Figure 20 As shown, specifically, a flow channel 684 is provided on the third layer 68, the flow channel 684 is connected to the receiving channel 681, and at least one of the first layer 61 and the second layer 62 and the channel wall of the flow channel 684 form a sample injection channel 66.

[0224] Specifically, when the flow channel 684 penetrates the thickness direction of the third layer 68, the wall of the flow channel 684, the first layer 61, and the second layer 62 form the sample inlet channel 66. When the flow channel 684 does not penetrate the thickness direction of the third layer 68, but penetrates the thickness direction of the third layer 68 on the side facing the first layer 61, the first layer 61 and the wall of the flow channel 684 form the sample inlet channel 66; when the flow channel 684 does not penetrate the thickness direction of the third layer 68, but penetrates the thickness direction of the third layer 68 on the side facing the second layer 62, the second layer 62 and the wall of the flow channel 684 form the sample inlet channel 66. The formation of the sample inlet channel 66 is simple and easy to manufacture.

[0225] An exhaust channel groove 685 is provided on the third layer 68. The exhaust channel groove 685 is connected to the receiving channel 681. At least one of the first layer 61 and the second layer 62 forms an exhaust channel 67 with the groove wall of the exhaust channel groove 685.

[0226] When the exhaust channel groove 685 does not penetrate the thickness direction of the third layer 68, and the side of the exhaust channel groove 685 facing the first layer 61 penetrates the thickness direction of the third layer 68, the first layer 61 and the groove wall of the exhaust channel groove 685 form an exhaust channel 67; when the exhaust channel groove 685 does not penetrate the thickness direction of the third layer 68, and the side of the exhaust channel groove 685 facing the second layer 62 penetrates the thickness direction of the third layer 68, the second layer 62 and the groove wall of the exhaust channel groove 685 form an exhaust channel 67. The formation of the exhaust channel 67 is simple and easy to process.

[0227] like Figure 20 As shown, when the inlet 64 and / or the outlet 65 are located on the side of the third layer 68, one end of the outlet channel 685 is connected to the receiving channel 681 and the other end is connected to the outlet channel 682; one end of the flow channel 684 is connected to the receiving channel 681 and the other end is connected to the inlet channel 683.

[0228] Figure 18As shown, when the inlet 64 is located in the first layer 61 or the second layer 62, or the outlet 65 is located in the first layer 61 or the second layer 62, one end of the outlet channel 685 is connected to the receiving channel 681, and the other end is directly opposite to the outlet 65 opened on the first layer 61 or the second layer 62; one end of the flow channel 684 is connected to the receiving channel 681, and the other end is directly opposite to the inlet 64 opened on the first layer 61 or the second layer 62.

[0229] Optionally, the venting groove 682, the injection groove 683, the flow channel groove 684 and / or the venting channel groove 685 can be formed by laser cutting. Of course, they can also be formed by injection molding, but laser cutting does not require injection molding of the third layer 68, so the cost is lower.

[0230] The first layer 61, the second layer 62 and the third layer 68 can be welded to the outer contour of the exhaust groove 682 (or exhaust port 65), the sample inlet groove 683 (or sample inlet 64), the flow channel groove 684 and / or the exhaust channel groove 685 by laser, thereby forming the sample inlet 64, the exhaust port 65, the sample inlet channel 66 and the exhaust channel 67.

[0231] like Figure 11 , Figure 17 and Figure 18 As shown, the carrier also includes a sealing structure to restrict the outflow of the reaction sample from the inlet 64 and / or the vent 65. Specifically, after liquid injection, the inlet 64 and / or the vent 65 can be sealed by the sealing structure to prevent contamination such as aerosols. Figure 17 , 18 As shown, optionally, the sealing structure may include a first sealing component 5 capable of restricting the outflow of the reaction sample from the vent port 65; and / or the sealing structure may include a second sealing component 4 capable of sealing the inlet port 64. When the distance between the vent port 65 and the inlet port 64 is large, or when the vent port 65 and the inlet port 64 are located on opposite sides of the carrier, the first sealing component 5 and the second sealing component 4 may be provided to connect to the vent port 65 and the inlet port 64 respectively. Figure 18 As shown, optionally, the first sealing assembly 5 includes a breathable but waterproof membrane 51 that is sealed to the exhaust port 65. The breathable but waterproof membrane 51 neither affects the air from the containment cavity 63 nor restricts the flow of the reaction sample from the breathable but waterproof membrane 51.

[0232] For example, in processing the carrier in this embodiment, the third layer 68 is laser-cut to form a receiving groove 681, an exhaust groove 682 (if the exhaust port 65 is located in the third layer 68), a sample inlet groove 683 (if the sample inlet 64 is located in the third layer 68), a flow channel groove 684, an exhaust channel groove 685, and a connecting portion 686. Then, the first layer 61 is welded to the third layer 68, and the second layer 62 is welded to the third layer 68 using a laser. The welding positions are around the carrier and the receiving groove 681, the exhaust groove 682 (if the exhaust port 65 is located in the third layer 68), and the sample inlet groove 686 formed in the previous step. 83 (if the inlet 64 is located in the third layer 68), flow channel 684, exhaust channel 685, inlet 64 (if the inlet 64 is located in the first layer 61 or the second layer 62), exhaust port 65 (if the exhaust port 65 is located in the first layer 61 or the second layer 62), outer contour of connecting part 686. If the inlet 64 and the exhaust port 65 are located on the first layer 61 and / or the second layer 62, the membrane material at the inlet 64 and the exhaust port 65 of the first layer 61 and / or the second layer 62 is removed by laser. Finally, the insert 32 is welded to the inlet 64 and / or the exhaust port 65.

[0233] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A vector, characterized in that, The device includes a main body (6,1,2), which includes a first layer (61,11,21) and a second layer (62,12,22) disposed opposite to each other. At least the second layer (62,12,22) is a flexible film. The first layer (61,11,21) and the second layer (62,12,22) form a receiving cavity (63,13,23). At least one connecting portion (686,111,211) is formed in the receiving cavity (63,13,23). The connecting portion (686,111,211) connects the first layer (61,11,21) and the second layer (62,12,22) to reduce the deformation of the receiving cavity (63,13,23).

2. The carrier of claim 1, wherein, The first layer (61, 11, 21) is formed by vacuum forming, injection molding or machining.

3. The carrier of claim 1, wherein, The first layer (11, 21) has a receiving slot (113, 213), which includes a connecting slot (1131, 2131) and at least two sub-slots (1132, 2132). Two adjacent sub-slots (1132, 2132) are connected through the connecting slot (1131, 2131). One sidewall of the receiving groove (113, 213) is provided with the connecting part (111, 211), and the space between the connecting part (111, 211) and the other sidewall of the receiving groove (113, 213) forms the communicating groove (1131, 2131); or The receiving groove (113, 213) has connecting portions (111, 211) on both side walls. The connecting portions (111, 211) on the two side walls are arranged opposite to each other, and the space between the connecting portions (111, 211) on the receiving groove (113, 213) forms the communicating groove (1131, 2131); or The two side walls of the receiving groove (113, 213) are provided with the connecting part (111, 211), and the connecting parts (111, 211) of the two side walls are arranged alternately. The space of the receiving groove (113, 213) between the connecting part (111, 211) and the other side wall of the receiving groove (113, 213) constitutes the communicating groove (1131, 2131). In a top-view orientation, the cross-sectional area of ​​the connecting slot (1131, 2131) is smaller than the cross-sectional area of ​​the sub-slot (1132, 2132); and / or The connecting part (111, 211) is a column structure. One end of the column structure is connected to the bottom of the receiving groove (113, 213), and the other end extends towards the opening of the receiving groove (113, 213). The column structure is located in the middle of the receiving groove.

4. The vector according to any one of claims 1 to 3, characterized in that, The first layer (11,21) is a flexible film.

5. The vector according to any one of claims 1 to 3, wherein The first layer (11,21) has a lower deformability than the second layer (12,22).

6. The vector of any one of claims 1-3, wherein, The first layer (11,21) and the second layer (12,22) are connected by laser welding, bonding or cryogenic bonding.

7. The carrier of claim 1, wherein The main body (6) further includes a third layer (68), the first layer (61) is a flexible film, the deformability of the third layer (68) is lower than that of the first layer (61) and the second layer (62), the third layer (68) has a receiving groove (681) extending through its thickness direction, the first layer (61) and the second layer (62) are respectively connected to the two sides of the thickness direction of the third layer (68), the receiving groove (681), the first layer (61) and the second layer (62) form the receiving cavity (63); the connecting part (686) extends from one side wall of the receiving groove (681) to the other side wall to form the connecting part (686), the connecting part (686) is respectively connected to the first layer (61) and the second layer (62).

8. The carrier according to claim 7, characterized in that, The receiving slot (681) includes a connecting slot (6811) and at least two sub-slots (6812), wherein two adjacent sub-slots (6812) are connected through the connecting slot (6811), wherein: One sidewall of the receiving channel (681) is provided with the connecting part (686), and the space between the connecting part (686) and the other sidewall of the receiving channel (681) constitutes the communicating channel (6811); or The receiving channel (681) has connecting portions (686) on both side walls, the connecting portions (686) of the two side walls are arranged opposite to each other, and the space between the connecting portions (686) of the receiving channel (681) of the two side walls constitutes the communicating channel (6811); or The two side walls of the receiving channel (681) are provided with the connecting part (686), and the connecting parts (686) of the two side walls are arranged alternately. The space of the receiving channel (681) between the connecting part (686) and the other side wall of the receiving channel (681) constitutes the communicating channel (6811). In a top view, the cross-sectional area of ​​the connecting channel (6811) is smaller than the cross-sectional area of ​​the sub-channel (6812).

9. The vector of any of claims 1-3 and 7-8, wherein, The number of the connecting parts (686, 111, 211) is multiple. The multiple connecting portions (686, 111, 211) may have the same or different shapes; and / or The plurality of connecting portions (686, 111, 211) are arranged along the length direction of the receiving cavity (63, 13, 23); and / or The sidewalls of the plurality of connecting portions (686, 111, 211) facing the receiving cavity (63, 13, 23) are arc-shaped; and / or The connection points between the multiple connecting parts (686, 111, 211) and the sidewalls of the receiving cavity (63, 13, 23) are arc-shaped.

10. The vector of any one of claims 1-3 and 7-8, wherein, The main body (6,1,2) is provided with an inlet (64,14,24) and / or an exhaust port (65,15,25) that communicate with the receiving cavity (63,13,23).

11. The carrier of claim 10, wherein, The sample inlet (64, 14, 24) and the exhaust outlet (65, 15, 25) are both located in the first layer (61, 11, 21) or the second layer (62, 12, 22); or The inlet (64,14,24) is located in one of the first layer (61,11,21) and the second layer (62,12,22), and the outlet (65,15,25) is located in the other of the first layer (61,11,21) and the second layer (62,12,22).

12. The carrier of claim 10, wherein, When the carrier includes a third layer (68), the inlet (64) and the outlet (65) are both located on the side of the third layer (68); or one of the inlet (64) and the outlet (65) is located on the side of the third layer (68), and the other is located in the first layer (61) or the second layer (62).

13. The carrier of claim 12, wherein, The third layer (68) is provided with an exhaust groove (682) and / or a sample inlet groove (683), the exhaust groove (682) and / or the sample inlet groove (683) are connected to the receiving cavity (63) and extend to the side of the third layer (68); At least one of the first layer (61) and the second layer (62) forms the exhaust port (65) with the wall of the exhaust groove (682), and / or at least one of the first layer (61) and the second layer (62) forms the injection port (64) with the wall of the injection groove (683).

14. The carrier of claim 10, wherein, The reaction sample enters the containment cavity (63,13) through the inlet (64,14) and the first end (631,131) of the containment cavity (63,13). The air in the containment cavity (63,13) enters the exhaust port (65,15) through the second end (632,132) of the containment cavity (63,13). The first end (631,131) and the second end (632,132) are arranged opposite to each other.

15. The carrier of claim 14, wherein, The first end (631, 131) and the second end (632, 132) are located between the exhaust port (65, 15) and the injection port (64, 14), with the injection port (64, 14) positioned closer to the first end (631, 131) and the exhaust port (65, 15) positioned closer to the second end (632, 132); or The exhaust port (65, 15) and the sample inlet (64, 14) are both located on the side of the receiving cavity (63, 13) closer to the first end (631, 131) or closer to the second end (632, 132).

16. The carrier of claim 15, wherein, The inlet (64, 14) is disposed opposite to the first end (631, 131) of the receiving cavity (63, 13), and the outlet (65, 15) is disposed opposite to the second end (632, 132) of the receiving cavity (63, 13).

17. The carrier of claim 14, wherein, The inlet (64, 14) and the receiving cavity (63, 13) are connected via the injection channel (66, 16); and / or The exhaust port (65, 15) is connected to the receiving cavity (63, 13) through the exhaust channel (67, 17).

18. The carrier of claim 17, wherein, One end of the injection channel (66, 16) is connected to the first end (631, 131), and the other end is connected to the injection port (64, 14); and / or One end of the exhaust passage (67, 17) is connected to the second end (632, 132), and the other end is connected to the exhaust port (65, 15).

19. The carrier of claim 17, wherein, When the main body (6) includes a first layer (61), a second layer (62) and a third layer (68), the third layer (68) is provided with a flow channel groove (684) and / or an exhaust channel groove (685), the flow channel groove (684) and / or the exhaust channel groove (685) are connected to the receiving channel (681), at least one of the first layer (61) and the second layer (62) and the groove wall of the exhaust channel groove (685) form the exhaust channel (67), and / or at least one of the first layer (61) and the second layer (62) and the groove wall of the flow channel groove (684) form the sample injection channel (66). When the main body (1) includes a first layer (11) and a second layer (12), the first layer (11) is provided with a flow channel groove (114) and / or an exhaust channel groove (115), the second layer (12) and the exhaust channel groove (115) form the exhaust channel (17), and / or the second layer (12) and the flow channel groove (114) form the sample inlet channel (16); or, when the main body (1) includes a first layer (11) and a second layer (12), two spaced first connecting strips (161) are formed between the first layer (11) and the second layer (12), and the sample inlet channel (16) is formed between the two first connecting strips (161); and / or, two spaced second connecting strips (171) are formed between the first layer (11) and the second layer (12), and the exhaust channel (17) is formed between the two second connecting strips (171).

20. The carrier of claim 10, wherein, The carrier also includes a sealing structure for restricting the flow of reaction sample from the inlet (64, 14, 24) and / or the outlet (65, 15, 25).

21. The carrier of claim 20, wherein, The sealing structure includes a first sealing component (5) capable of restricting the outflow of the vent (65, 15, 25) from the reaction sample; and / or the sealing structure includes a second sealing component (4) capable of sealing the inlet (64, 14, 24); or The sealing structure includes a third sealing component (3) capable of sealing the exhaust port (65, 15, 25) and the sample inlet (64, 14, 24).

22. The carrier of claim 21, wherein, The first sealing assembly (5) includes a breathable but waterproof membrane (51) that is sealed to the exhaust port (65,15,25).

23. The carrier of claim 21, wherein, The sealing structure is capable of compressing the air within the carrier and causing the first layer (61, 11, 21) and / or the second layer (62, 12, 22) to protrude outwards.

24. The carrier of claim 23, wherein, The sealing structure includes: The inserted part (32) is sealed to the body (6,1) and covers the exhaust port (65,15) and / or the injection port (64,14). The inserted part (32) has a sealed cavity (321) communicating with the exhaust port (65,15) and / or the injection port (64,14). The seal (31) is at least partially capable of sealing the cavity (321) inserted into it to at least compress the air in the cavity (321) and to cause the first layer (61, 11) and / or the second layer (62, 12) to protrude outward.

25. The carrier of claim 24, wherein, The seal (31) is capable of sealing the air inserted into the sealing cavity (321) to compress the air in the sealing cavity (321) and the receiving cavity (63,13).

26. The carrier of claim 7, wherein, The thickness of the third layer (68) is less than 0.5 mm, and / or The third layer (68) is made of polycarbonate, polypropylene, polyimide or polyethylene material, and / or The third layer (68) is made of transparent material.

27. The carrier of claim 26, wherein, The thickness of the third layer (68) is less than 0.3 mm.

28. The vector of any one of claims 1-3 and 7-8, wherein, The thickness of the flexible film is less than 0.2 mm.

29. The carrier of claim 28, wherein, The thickness of the flexible film is less than 0.1 mm or less than 0.05 mm.

30. The vector of any one of claims 1-3 and 7-8, wherein, The first layer (61, 11, 21) and / or the second layer (62, 12, 22) are made of transparent material.

31. The vector of any of claims 1-3, 4, and 7, wherein, The first layer (61, 11, 21) and / or the second layer (62, 12, 22) are polycarbonate films, polypropylene films, or polyimide films; or The first layer (61, 11, 21) or the second layer (62, 12, 22) is an aluminum film; or The first layer (61, 11, 21) or the second layer (62, 12, 22) includes an aluminum film and an isolation film, wherein the isolation film is connected to the inner side of the aluminum film.

32. The carrier of claim 7, wherein, The first layer (61) and the third layer (68), and the second layer (62) and the third layer (68) are connected by laser welding, bonding or low-temperature bonding, and / or The third layer (68) is formed by machining or injection molding.

33. The vector of any one of claims 1-3 and 7-8, wherein, The accommodating cavities (63, 13, 23) have a flat structure.

34. The carrier of claim 33, wherein, The flat structure refers to the fact that the dimension of the receiving cavity (63,13,23) in the direction perpendicular to its thickness direction is greater than its dimension in the thickness direction.

35. The vector of claim 34, wherein, The ratio of the dimension of the receiving cavity (63,13,23) perpendicular to its thickness direction to its dimension in the thickness direction is greater than 5:

1.

36. The vector of claim 35, wherein, The size ratio is 50:1 to 100:1.

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