Cell centrifugal collection device

Abstract

The utility model relates to a cell centrifugal collection device which comprises a collection box and a connection channel, the collection box is of an integrated structure and comprises a box cover and a box body, the box cover is rotatably connected with one side of the box body, a buckle is arranged on the edge of the box cover to buckle the box cover and the box body, the connection channel comprises a connection block and a rubber sealing ring, the connection block is fixed below the box body, and the rubber sealing ring is fixed below the connection block. An L-shaped hole channel is formed in the connecting block and communicates with the inner side of the box body, and the rubber sealing ring is fixedly installed on the outer side of the connecting position of the L-shaped hole channel and the inner side of the box body. The cell centrifugal collection device is good in sealing performance, not prone to leakage and capable of remarkably improving the collection rate of cells, the spiral threads are arranged in the funnel portion of the L-shaped hole channel, the vortex effect is formed in the centrifugal state, and the cells are evenly coated.

Description

Technical Field

[0001] This utility model relates to the field of medical testing equipment, specifically to a cell centrifugation collection device. Background Technology

[0002] In medicine, body fluids containing cells are often subjected to cell counting to determine the number of cells per unit volume of sample. The amount of sample to be added is determined by the pore size of the absorbent paper pad in the cell collector, based on the diameter of different cell types. This ensures that the cells are evenly distributed within the pore size of the absorbent paper pad without cell overlap, thereby identifying pathogenic cells and the cause of the disease.

[0003] Existing technology CN212031078U discloses a glass cell collection device; however, its guide tube has a straight cylindrical structure, resulting in low cell recovery and collection rates, only 30-40% in actual clinical use. The sample vial cap and vial are threaded together, which may cause leakage of cell-containing liquid during centrifugation. Furthermore, the upper and lower smear clamps use a hinged structure, resulting in poor sealing performance and potential sample leakage during centrifugation, generating aerosols and posing a biosafety hazard. In addition, existing collection devices are all reusable, with most products using effective chlorine immersion for disinfection, which affects their lifespan and results in incomplete disinfection, potentially leading to cell contamination. Moreover, existing collection devices often fail to calculate the liquid volume and the absorbent paper's saturation point, leading to excessive sample volume, incomplete centrifugation, and low cell collection efficiency. Excessive liquid volume can also cause sample overflow and splashing during centrifugation due to oversaturated absorbent paper, also posing a biosafety hazard. Existing collection devices also exhibit problems such as densely packed cells at the edges and sparsely distributed cells in the center, resulting in uneven cell collection and spreading. In addition, some existing disposable collectors lack a sealing ring design, which reduces the collection rate of centrifuged cells and can also cause sample spillage and splashing, leading to biosafety hazards. Utility Model Content

[0004] To address the technical problems existing in the prior art, this utility model provides a cell centrifugation collection device.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A cell centrifugation collection device includes a collection box and a connecting channel. The collection box is an integral structure, including a lid and a body. The lid is rotatably connected to one side of the body. The edge of the lid is provided with a buckle to fasten the lid to the body. The connecting channel includes a connecting block and a rubber sealing ring. The connecting block is fixed to the bottom of the body. The connecting block has an L-shaped channel that communicates with the inner side of the body. The rubber sealing ring is fixedly installed on the outside of the connection between the L-shaped channel and the inner side of the body.

[0007] As a preferred technical solution, the L-shaped channel includes a funnel-shaped part, an arc-shaped connecting part, and a pipe hole. The larger side of the funnel-shaped part is set inside the opening of the rubber sealing ring, and the other side is connected to the pipe hole through the arc-shaped connecting part. The axis of the funnel-shaped part is perpendicular to the pipe hole.

[0008] As a preferred technical solution, the inner wall of the funnel is provided with spiral patterns.

[0009] As a preferred technical solution, a pipe cap is provided at the outer end of the pipe hole.

[0010] As a preferred technical solution, the number of connection channels is one or more.

[0011] As a preferred technical solution, the top of the rubber sealing ring protrudes from the inner surface of the box.

[0012] As a preferred technical solution, a rectangular protrusion is provided on the inside of the box lid.

[0013] As a preferred technical solution, when the device is in use, the lid and the body are fastened together, filter paper is placed on one side of the body and glass slide is placed on the other side of the lid. The filter paper and glass slide are clamped together by a rubber sealing ring and a rectangular protrusion, with the outer end of the L-shaped channel facing upward.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] This novel cell centrifugation collection device features an L-shaped channel with the inlet facing upwards during use, preventing liquid from flowing out of the inlet during centrifugation. The funnel design of the L-shaped channel allows liquid to flow along the inclined inner wall of the funnel into the collection box. The spiral pattern on the inner wall of the funnel conforms to the fluid dynamics of centrifugation, ensuring that cells are concentrated and evenly distributed on the glass slide. The collection box has a one-piece structure, and the filter paper and glass slide are clamped together internally by a sealing ring and rectangular protrusions, improving the device's airtightness. Through these structural designs, preliminary experiments using this device have shown that the cell recovery rate can be increased to 55-60%.

[0016] This practical cell centrifugation collection device requires prior low-speed centrifugation (1000 rpm, approximately 120-200 g, for 10 minutes) before use. The centrifuged liquid (250 μL-300 μL) is then thoroughly mixed and added to the device for centrifugation (centrifugal force approximately 120-200 g, for 10 minutes). The centrifugal force and centrifugation time can be adjusted according to the cell density and liquid viscosity of different samples to ensure complete absorption of the liquid and prevent oversaturation and overflow. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of the cell centrifugation collection device of this utility model;

[0018] Figure 2 This is a side view of the cell centrifugation collection device of this utility model.

[0019] In the diagram: 1. Collection box; 11. Box lid; 12. Box body; 13. Buckle; 2. Rubber sealing ring; 3. Connecting block; 31. Funnel part; 32. Arc-shaped connecting part; 33. Pipe hole; 34. Pipe cover. Detailed Implementation

[0020] The technical solution of this utility model will be further described below with reference to specific embodiments:

[0021] like Figure 1 As shown, a cell centrifugation collection device includes a collection box 1 and connecting channels. The collection box 1 is an integral structure, including a lid 11 and a body 12. The lid 11 is rotatably connected to one side of the body 12. The edge of the lid 11 is provided with a buckle 13 to fasten the lid 11 to the body 12. There are one or more connecting channels, each including a connecting block 3 and a rubber sealing ring 2. The connecting block 3 is fixed to the bottom of the body 12, and the connecting block 3 has an L-shaped channel that communicates with the inside of the body 12. The rubber sealing ring 2 is fixedly installed on the outside of the connection between the L-shaped channel and the inside of the body 12.

[0022] The L-shaped channel includes a funnel portion 31, an arc-shaped connecting portion 32, and a tube hole 33. The larger side of the funnel portion 31 is positioned inside the opening of the rubber sealing ring 2, while the other side communicates with the tube hole 33 via the arc-shaped connecting portion 32. The axis of the funnel portion 31 is perpendicular to the tube hole 33. In use, the funnel portion 31 is horizontally positioned, allowing liquid to flow along the inclined inner wall of the funnel portion 31 towards the collection box 1. In this embodiment, the width of the funnel portion 31 near the box body 12 is 7 mm, and the width of the other side is 5 mm. The diameter of the tube hole 33 is 5 mm.

[0023] The L-shaped channel includes a funnel-shaped part 31, an arc-shaped connecting part 32, and a pipe hole 33. The larger side of the funnel-shaped part 31 is located inside the opening of the rubber sealing ring 2, and the other side is connected to the pipe hole 33 through the arc-shaped connecting part 32. The axis of the funnel-shaped part 31 is perpendicular to the pipe hole 33.

[0024] The inner wall of the funnel 31 is provided with spiral patterns. By setting the spiral patterns, a vortex effect can be formed during low-speed centrifugation, which conforms to the fluid dynamics of the centrifugation process and allows the cells to be concentrated and evenly distributed on the glass slide.

[0025] The outer end of the tube 33 is provided with a tube cap to cover the liquid inlet, preventing biological samples from spilling out and reducing aerosol generation.

[0026] like Figure 2As shown, the top of the rubber sealing ring 2 protrudes from the inner surface of the box body 12. A rectangular protrusion is provided on the inner side of the box cover 11. When the device is in use, the box cover 11 is fastened to the box body 12, filter paper is placed on one side of the box body 12, and a glass slide is placed on one side of the box cover 11. The filter paper and glass slide are clamped together by the rubber sealing ring 2 and the rectangular protrusion to improve the sealing and reduce cell leakage.

[0027] When using the device, the outer end of the L-shaped channel should face upwards to prevent liquid containing cells from flowing out of the inlet.

[0028] This embodiment is merely a further explanation of the present invention and is not intended to limit the present invention. After reading this specification, those skilled in the art can make non-creative modifications to this embodiment as needed, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A cell centrifugation collection device, comprising: The device includes a collection box and a connecting channel. The collection box is an integral structure, including a lid and a body. The lid is rotatably connected to one side of the body. The lid edge is provided with a buckle to fasten the lid to the body. The connecting channel includes a connecting block and a rubber sealing ring. The connecting block is fixed at the bottom of the body. The connecting block has an L-shaped channel that communicates with the inside of the body. The rubber sealing ring is fixedly installed on the outside of the connection between the L-shaped channel and the inside of the body.

2. The cell centrifugation collection device of claim 1, wherein, The L-shaped channel includes a funnel-shaped part, an arc-shaped connecting part, and a pipe hole. One side of the funnel-shaped part is set inside the opening of the rubber sealing ring, and the other side is connected to the pipe hole through the arc-shaped connecting part. The axis of the funnel-shaped part is perpendicular to the pipe hole.

3. The cell centrifugation collection device of claim 2, wherein, The inner wall of the funnel section is provided with spiral patterns.

4. The cell centrifugation collection device of claim 2, wherein, The outer end of the pipe hole is provided with a pipe cap.

5. The cell centrifugation collection device of claim 1, wherein, The number of connection channels is one or more.

6. The cell centrifugation collection device of claim 1, wherein, The top of the rubber sealing ring protrudes from the inner surface of the box.

7. The cell centrifugation collection device of claim 1, wherein, The inner side of the box lid has a rectangular protrusion.

8. The cell centrifugation collection device of claim 7, wherein, When the device is in use, the lid is fastened to the body, filter paper is placed on one side of the body, and a glass slide is placed on the other side of the lid. The filter paper and glass slide are clamped together by a rubber sealing ring and a rectangular protrusion, with the outer end of the L-shaped channel facing upward.

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