Plasma separating device

Abstract

The utility model provides a plasma separating device. The plasma separating device comprises a gland, an elastic sealing ring, a sample adding seat, a filtering element and a base, wherein an annular groove is formed in the peripheral side wall of the gland, and the elastic sealing ring is arranged in the annular groove; a sample adding through hole is formed in the sample adding seat, the gland is slidably connected into the sample adding through hole in the vertical direction, and the elastic sealing ring is in interference fit with the hole wall of the sample adding through hole; the base is provided with a bracket and a collecting chamber, the bracket is matched with and accommodates the sample adding seat, and the filter element is arranged at the bottom of the bracket and matched with and corresponds to the sample adding through hole; the bottom of the bracket is provided with a channel communicated with the collecting chamber. A collected whole blood sample is injected into the sample adding through hole, the filtering element is wetted by the whole blood, an operator presses the gland into the sample adding through hole in the vertical direction, the elastic sealing ring arranged on the gland is in interference fit with the sample adding through hole to form sealing, and the gland pushes air in the sample adding through hole to drive the whole blood to be filtered by the filtering element to separate plasma. And the plasma enters a collection chamber through a channel arranged at the bottom of the bracket and is used for detection in the next step.

Description

Technical Field

[0001] This utility model belongs to the field of biological detection technology, specifically relating to a plasma separation device. Background Technology

[0002] Blood test samples are mainly divided into whole blood, serum, and plasma. The pale yellow liquid obtained after removing blood cells is plasma, primarily used for coagulation and immunological tests. Traditionally, plasma samples are obtained by separating whole blood using low-speed centrifugation. This method requires a certain volume of blood, and the sample preparation process is highly specialized, complex, time-consuming, and requires additional equipment such as a large, low-temperature benchtop centrifuge. However, centrifuges need to be installed in a fixed, level location and are not portable. Recently, other techniques for separating plasma have emerged, such as filtration. Filtration utilizes the physical size difference between hemoglobin and plasma, designing corresponding pores to allow plasma to pass through while trapping hemoglobin, thus obtaining the desired plasma. In filtration, plasma generally flows gently downwards due to gravity. If the liquid driving force is insufficient or the blood sample volume is large, the plasma may not flow in the predetermined manner. Utility Model Content

[0003] In view of this, the purpose of this utility model is to provide a plasma separation device that can provide sufficient liquid driving force and quickly complete plasma filtration.

[0004] The present invention discloses a plasma separation device, comprising: a pressure cap, an elastic sealing ring, a sample dispensing seat, a filter element, and a base;

[0005] The outer peripheral sidewall of the pressure cap is provided with an annular groove, and the elastic sealing ring is disposed in the annular groove;

[0006] The sample feeding seat is provided with a sample feeding through hole, the pressure cap is slidably connected to the sample feeding through hole along the vertical direction, and the elastic sealing ring is interference-fitted with the hole wall of the sample feeding through hole;

[0007] The base is provided with a slot and a collection chamber. The slot is matched to accommodate the sample dispensing seat. The filter element is disposed at the bottom of the slot and matches the sample dispensing through hole. The bottom of the slot is provided with a channel communicating with the collection chamber.

[0008] The collected whole blood sample is injected into the sample dispensing orifice, and the whole blood wets the filter element. The operator presses the cap vertically into the sample dispensing orifice. The elastic sealing ring on the cap is press-fitted into the sample dispensing orifice to form a seal. The cap pushes the air in the sample dispensing orifice to drive the whole blood through the filter element to separate the plasma. The plasma enters the collection chamber through the channel at the bottom of the tray for further testing. This device quickly completes the micro-volume whole blood plasma filtration and separation process.

[0009] Furthermore, the bottom perimeter of the pressure cap is rounded to reduce the resistance of the sliding connection of the pressure cap.

[0010] Furthermore, the top of the cap is provided with a frustum, and when the frustum abuts against the top of the tray, there is a gap between the bottom surface of the cap and the filter element. The frustum controls the pressing height of the cap, avoiding direct compression of the filter element and affecting the filtration effect.

[0011] Furthermore, the sidewall of the tray is provided with several vertical ribs, the cross-sectional area of ​​which gradually increases downwards. The ribs on the tray act as dampers, and during the pressing of the cap, the sample holder is blocked by the ribs. The operator can slowly press down the cap by hand to avoid excessive pressure and breakage of hemoglobin.

[0012] Furthermore, the plasma separation device disclosed in this utility model also includes a detection chip, which is slidably connected to the collection chamber and corresponds to the channel. Integrating the detection chip into the separation device allows for direct transfer of the separated plasma onto the detection chip, simplifying the transfer operation.

[0013] Furthermore, the detection chip includes a sample application area and a detection area, with the sample application area corresponding to the channel and connected to the detection area. The sample application area is responsible for receiving samples, ensuring correct sample entry. Separating the sample application area and the detection area avoids cross-contamination or interference, which helps improve detection accuracy.

[0014] This utility model has the following beneficial effects:

[0015] 1) The plasma separation device provided by this utility model compresses the air in the cavity formed by the pressure cap, the sample dispensing hole and the filter element by applying pressure to the pressure cap, thereby providing sufficient liquid driving force to quickly separate plasma and achieve whole blood filtration. It is simple and convenient to operate and has high separation efficiency.

[0016] 2) The plasma separation device provided by this utility model has a simple structure, small size and light weight, and can be carried by hand, which is convenient for personnel to use and reduces the workload of medical staff; it can directly obtain plasma samples without the need for other hemoglobin separation instruments, and the cost is low.

[0017] 3) The plasma separation device provided by this utility model is suitable for the separation and detection of trace amounts of whole blood and has wide applicability. Attached Figure Description

[0018] Figure 1 This is an overall schematic diagram of the plasma separation device provided in some embodiments of this utility model.

[0019] Figure 2 This is a front view of a plasma separation device provided in some embodiments of this utility model.

[0020] Figure 3 This is a left view of a plasma separation device provided in some embodiments of this utility model.

[0021] Figure 4 This is a top view of a plasma separation device provided in some embodiments of this utility model.

[0022] Figure 5 This is an explosion diagram of a plasma separation device provided in some embodiments of this utility model.

[0023] Figure 6 This is an assembly diagram of some components of the plasma separation device provided in some embodiments of this utility model.

[0024] Figure 7 This is an assembly diagram of some components of the plasma separation device provided in some embodiments of this utility model.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. Capping, 2. Elastic sealing ring, 3. Sample dispensing seat, 4. Filter element, 5. Base, 6. Channel, 7. Sample dispensing area, 8. Detection area, 9. Detection chip, 10. Rib, 11. Collection chamber, 12. Slot, 13. Sample dispensing through hole. Detailed Implementation

[0027] To more clearly and completely describe the technical solution of this utility model, the following detailed description is provided through specific embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the utility model. Various modifications can be made within the scope of the claims of this utility model. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model pertains. The terminology used in this specification of this utility model is for the purpose of describing particular embodiments only and is not intended to limit the utility model.

[0028] like Figure 1-6 As shown, this utility model provides a plasma separation device, including a pressure cap 1, an elastic sealing ring 2, a sample dispensing seat 3, a filter element 4, and a base 5; the outer wall of the pressure cap 1 is provided with an annular groove, and the elastic sealing ring 2 is disposed in the annular groove, as shown. Figure 7As shown, the pressure cap 1 is equipped with an elastic sealing ring 2 for later use; the sample dispensing seat 3 has a sample dispensing through hole 13, and the pressure cap 1 is vertically slidably connected to the sample dispensing through hole 13. The bottom periphery of the pressure cap 1 is rounded to reduce the resistance of the sliding connection of the pressure cap 1. The elastic sealing ring 2 is interference-fitted with the hole wall of the sample dispensing through hole 13; the base 5 has a tray 12 and a collection chamber 11. The tray 12 is matched to accommodate the sample dispensing seat 3, and the filter element 4 is disposed at the bottom of the tray 12 and matches the sample dispensing through hole 13; the filter element 4 can be made of a suitable material that can use pressure difference to separate the supplied blood into hemoglobin and plasma. Filter materials with this function include, for example, filter materials in which a large number of fine fibers are wound, porous filter materials, etc. Fibers include suitable synthetic fibers and natural polymer fibers, such as fibers made of polyester, polyethylene, polypropylene, polyamide, cellulose, cotton, flax or silk. Alternatively, glass fiber, etc., can be used. The aforementioned materials can be used to fabricate a blood filtration membrane as filter element 4, which separates plasma and hemoglobin. Based on the physical sizes of hemoglobin, red blood cells, and plasma, the blood filtration membrane is designed with pores of a specific diameter to block hemoglobin and other components while allowing plasma to pass through. The membrane undergoes chemical treatment to make red blood cells clump together and less prone to breakage. The top of the cap 1 has a frustum. When the frustum abuts against the top of the tray 12, a gap exists between the bottom surface of the cap 1 and the filter element 4. The frustum controls the downward pressure height of the cap 1, preventing direct compression of the filter element 4 and thus affecting the filtration effect.

[0029] The sidewall of the tray 12 has several vertically arranged ribs 10, with the cross-sectional area of ​​the ribs gradually increasing downwards. The ribs 10 act as dampers; during the pressing of the cap 1, the sample dispensing seat 3 is blocked by the ribs 10, allowing the operator to slowly press down the cap 1 by feel, preventing excessive pressure and breakage of hemoglobin. The bottom of the tray 12 has a channel 6 communicating with the collection chamber 11. The detection chip 9 is slidably connected to the collection chamber 11, corresponding to the channel 6. The detection chip 9 includes a sample dispensing area 7 and a detection area 8. The sample dispensing area 7 corresponds to the channel 6, and the sample dispensing area 7 communicates with the detection area 8; the communication structure can be a capillary tube.

[0030] The specific operating procedure of this device is as follows: The operator uses a pipette to add whole blood through the sample application port 13 into the sample application base 3. The whole blood wets the blood filter membrane located at the bottom of the sample application base 3. Then, the cap 1 equipped with the sealing elastic ring 2 is vertically pressed into the sample application port 13. The sealing elastic ring 2 and the sample application port 13 form an interference seal. The sealing elastic ring 2 on the cap 1, the sample application port 13, and the blood filter membrane in the tray 12 together form a sealed chamber. The operator applies downward pressure to press the cap 1 down, and the tray 12... The protruding ribs 10 on the side wall act as damping ribs, providing resistance during the pressing process, allowing the operator to press down slowly. By compressing the air in the sealed chamber, the air pressure provides the liquid driving force to filter the whole blood. Hemoglobin, red blood cells, etc. are intercepted on the blood filtration membrane, and high-quality plasma flows out through channel 6. The detection chip 9 placed in the collection chamber 11 corresponds to channel 6. The plasma flows into the sample application area 7 on the detection chip 9 and enters the detection area 8 through the capillary channel for detection, thus completing the micro-volume whole blood plasma separation detection process.

[0031] The plasma separation device and method provided by this utility model have been described in detail above. Specific examples have been used to illustrate the principle and implementation of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the core idea of ​​this utility model. It should be noted that those skilled in the art can make several improvements and modifications to this utility model without departing from the principle of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. A plasma separation device, characterized by, include: The components include: a pressure cap (1), an elastic sealing ring (2), a sample dispensing seat (3), a filter element (4), and a base (5); The outer peripheral sidewall of the pressure cap (1) is provided with an annular groove, and the elastic sealing ring (2) is disposed in the annular groove; The sample feeding seat (3) is provided with a sample feeding through hole (13), the pressure cap (1) is slidably connected in the sample feeding through hole (13) along the vertical direction, and the elastic sealing ring (2) is interference fit with the hole wall of the sample feeding through hole (13); The base (5) is provided with a tray (12) and a collection chamber (11). The tray (12) is matched to accommodate the sample dispensing seat (3). The filter element (4) is disposed at the bottom of the tray (12) and matches the sample dispensing through hole (13). The bottom of the tray (12) is provided with a channel (6) that communicates with the collection chamber (11).

2. The plasma separation device of claim 1, wherein The bottom perimeter of the cap (1) is rounded.

3. The plasma separation device of claim 1, wherein, The top of the cap (1) is provided with a frustum. When the frustum abuts against the top of the tray (12), there is a gap between the bottom surface of the cap (1) and the filter element (4).

4. The plasma separation device of claim 1, wherein, The sidewall of the bracket (12) is provided with several protruding ribs (10) along the vertical direction, and the cross-sectional area of ​​the protruding ribs (10) gradually increases downward.

5. Plasma separation device according to any one of claims 1-4, characterized in that It also includes a detection chip (9), which is slidably connected to the collection chamber (11) and corresponds to the channel (6).

6. The plasma separation device of claim 5, wherein the housing is formed of a material that is transparent to the light. The detection chip (9) includes a sample application area (7) and a detection area (8), wherein the sample application area (7) corresponds to the channel (6); the sample application area (7) is connected to the detection area (8).

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