Full-automatic immunoblotting analyzer capable of detecting while arriving

Abstract

The full-automatic immunoblotting analyzer comprises a shell, an analyzer main body and a control system, the analyzer main body comprises a base, a sample and reagent loading module, a pipetting module, an incubation reaction module, an air drying module and an image acquisition module, the pipetting module comprises a three-dimensional arm, a pipetting needle assembly and a liquid adding and absorbing assembly, the three-dimensional arm is connected with the control system, and the control system is connected with the control system. The pipetting needle assembly comprises a sample needle and a reagent needle, the liquid adding and sucking assembly comprises a first pipe nozzle and a second pipe nozzle, and the three-dimensional arm can simultaneously drive the sample needle, the reagent needle, the first pipe nozzle, the second pipe nozzle, the air drying module and the image acquisition module to simultaneously move in the X-axis direction above the base; the sample needle and the reagent needle can be driven to move in the Y-axis direction above the base at the same time, and the sample needle and the reagent needle can be driven to move in the Z-axis direction above the base respectively. The full-automatic immunoblotting analyzer capable of detecting at any time is simple and compact in internal structure, high in integration level, small in overall size and high in portability.

Description

Technical Field

[0001] This utility model belongs to the technical field of immunoblotting analysis instruments, specifically relating to a fully automated immunoblotting analyzer that can perform tests on demand. Background Technology

[0002] Immunoblotting, also known as Western blotting, is a method for detecting a specific protein in complex samples based on the specific binding of antigens and antibodies. Immunoblotting analyzers are essential instruments for immunoassays, primarily used in immunological research and clinical diagnosis. Immunoblotting involves multiple modules and complex experimental procedures; for example, pipetting includes adding samples, reagents, diluting the secondary antibody with diluent, and transferring waste liquid. Existing conventional immunoblotting analyzers have complex internal structures, making maintenance and repair difficult. Furthermore, their integration and space utilization are low, resulting in large overall sizes and reduced portability. Some smaller immunoblotting analyzers may lack automatic secondary antibody dilution (requiring manual dilution before the experiment) or have low throughput. In addition, existing conventional immunoblotting analyzers struggle to precisely calibrate the injection volume of sample and reagent needles before testing, compromising the accuracy of the results. Summary of the Invention

[0003] The purpose of this invention is to provide a fully automated immunoblot analyzer that is compact, highly integrated, and has high space utilization, allowing for on-demand testing.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] This utility model provides a fully automated immunoblotting analyzer for on-demand testing, comprising an openable cover housing, an analyzer body, and a control system. The analyzer body includes a base, a sample and reagent loading module, a pipetting module, an incubation reaction module, a drying module, and an image acquisition module mounted on the base. The base has a receiving cavity and a groove extending laterally from the top. The incubation reaction module is located in front of the groove, and the sample and reagent loading module is located behind the groove. The pipetting module includes a three-dimensional arm, a pipetting needle assembly, and a liquid aspiration assembly. The components include a sample needle and a reagent needle mounted on the three-dimensional arm. The liquid aspiration assembly includes a first nozzle and a second nozzle mounted on the three-dimensional arm. The drying module and the image acquisition module are both mounted on the three-dimensional arm. The three-dimensional arm can simultaneously move the sample needle, reagent needle, first nozzle, second nozzle, drying module, and image acquisition module above the base along the X-axis, simultaneously move the sample needle and reagent needle above the base along the Y-axis, and separately move the sample needle and reagent needle above the base along the Z-axis. This fully automated immunoblotting analyzer for on-demand testing has a simple, compact, and highly integrated internal structure, resulting in a small overall size and improved portability.

[0006] In an embodiment of this invention, the incubation reaction module includes a waste liquid tank, a shaker, and multiple incubation trays arranged sequentially on the shaker in a left-right direction. The shaker includes a bed plate mounted on the top outer surface of the base, a shaker motor mounted on the top inner surface of the base, and a transmission component disposed between the bed plate and the shaker motor. The bed plate has multiple first through holes arranged side-by-side in a left-right direction, and the base has second through holes corresponding to the positions of the first through holes. The first and second through holes cooperate to place reagent tubes. Each incubation tray includes multiple strip-shaped incubation tanks arranged side-by-side in a left-right direction. Through this configuration, combined with the control program design, the fully automated immunoblotting analyzer of this invention enables on-demand testing and multi-item testing of a single sample. This setting allows for precision calibration of the sample needle and reagent needle before testing, ensuring the accuracy of the test results. Specifically, before testing, the incubation tray is removed, and the control system controls the pipette assembly to add several sample or reagent needles to the reagent tube. The volume of liquid in the reagent tube is counted, and the volume of each sample or reagent needle is calculated, which is the injection volume.

[0007] Furthermore, the top of the base located in front of the groove is an inclined surface that gradually slopes downward from the groove. Fixed seats are provided on the inclined surface on the left and right sides of the bed board of the rocker, respectively. The rear end of the bed board of the rocker is pivotally connected to the fixed seats.

[0008] In this embodiment of the invention, the drying module and the image acquisition module are always located above the incubation reaction module. The drying module includes a fan, a robotic arm, and a robotic arm support. The fan is installed at the end of the robotic arm, and the robotic arm is fixedly connected to the three-dimensional arm through the support. The image acquisition module includes a camera, a reflector, an illumination device, and image acquisition and interpretation software. The camera, reflector, and illumination device are fixedly connected to the three-dimensional arm through the support.

[0009] In an embodiment of this utility model, the sample and reagent loading module includes an embedded sample rack and an embedded reagent rack disposed on the base. The embedded sample rack has sample tube placement positions arranged in a matrix for placing sample tubes, and the embedded reagent rack has reagent bottle placement positions arranged in a matrix for placing reagent bottles.

[0010] In an embodiment of this invention, the pipette assembly further includes a first plunger pump connected to the sample needle via a tubing and a second plunger pump connected to the reagent needle via a tubing, both mounted on the three-dimensional arm.

[0011] The liquid aspiration assembly also includes a peristaltic pump connected to the first nozzle via a hose and a diaphragm pump connected to the second nozzle via a hose, both disposed in the receiving cavity.

[0012] According to some specific embodiments of this utility model, the three-dimensional arm includes an X-axis moving support assembly, a Y-axis moving assembly, and a Z-axis moving assembly. The X-axis moving support assembly includes a support base fixedly installed in the groove, an X-axis guide rail installed on the support base, a connecting plate extending along the Z-axis direction and slidably connected to the X-axis guide rail, and a side plate extending along the Y-axis direction and vertically arranged and fixedly connected to the upper end of the connecting plate. The Y-axis moving assembly includes a mounting base installed on one side surface of the side plate, a Y-axis guide rail disposed on the mounting base, and a Z-axis base plate slidably connected to the Y-axis guide rail. The Z-axis moving assembly includes a first Z-axis guide rail and a second Z-axis guide rail disposed on the Z-axis base plate, a sample needle fixing seat slidably connected to the first Z-axis guide rail, and a reagent needle fixing seat slidably connected to the second Z-axis guide rail.

[0013] Furthermore, the sample needle is vertically mounted on the sample needle holder, the reagent needle is vertically mounted on the holder, and the liquid aspiration assembly also includes a nozzle bracket rotatably connected to the lower front part of the mounting base. The first nozzle and the second nozzle are respectively fixedly mounted on the nozzle bracket. The Y-axis moving assembly is disposed on one side surface of the side plate, and the drying module, the image acquisition module, the first plunger pump, and the second plunger pump are all disposed on the other side surface of the side plate.

[0014] Furthermore, a scanning device and a cleaning tank are installed on the connecting plate. The cleaning tank is located in the groove, and the top of the cleaning tank is flush with the top of the base. The scanning device is located in the receiving cavity. A rotating wheel is provided at the bottom of the mounting base. The lower surface plate of the rotating wheel contacts the upper surface of the base, and the rotation axis of the rotating wheel extends along the Y-axis.

[0015] In an embodiment of this utility model, the X-axis moving support assembly further includes a first driving assembly for driving the connecting plate to move along the X-axis direction. The first driving assembly includes a first synchronous belt mounted on the support base, a first motor mounted on the support base capable of driving the first synchronous belt to move, and a first slider mounted on the connecting plate. The first slider is slidably connected to the X-axis guide rail, and the connecting plate is fixedly connected to the first synchronous belt. The Y-axis moving assembly further includes a second driving assembly for driving the Z-axis base plate to move along the Y-axis direction. The second driving assembly includes a second synchronous belt mounted on the mounting base, a second motor mounted on the mounting base capable of driving the second synchronous belt to move, and a second slider mounted on the Z-axis base plate. The second slider is slidably connected to the Y-axis guide rail, and the Y-axis guide rail is mounted on the side of the mounting base. The second synchronous belt is located in the mounting base. A through groove extending along the Y-axis is provided on the side of the mounting base. The bottom of the Z-axis base plate is fixedly connected to the second synchronous belt through a connecting rod located in the through groove. The Z-axis moving assembly also includes a third driving assembly for driving the sample needle holder to move along the Z-axis and a fourth driving assembly for driving the reagent needle holder to move along the Z-axis. The third driving assembly includes a first electric lead screw mounted on the Z-axis base plate and a third slider mounted on the sample needle holder and connected to the first electric lead screw. The third slider is slidably connected to the first Z-axis guide rail. The fourth driving assembly includes a second electric lead screw mounted on the Z-axis base plate and a fourth slider mounted on the reagent needle holder and connected to the second electric lead screw. The fourth slider is slidably connected to the second Z-axis guide rail.

[0016] In the embodiments of this utility model, position sensors are respectively provided on the X-axis guide rail, Y-axis guide rail, first Z-axis guide rail, and second Z-axis guide rail.

[0017] Furthermore, the X-axis guide rail includes an upper X-axis guide rail mounted on the lower part of the support base and a lower X-axis guide rail mounted on the upper part of the support base. The synchronous belt is disposed between the upper X-axis guide rail and the lower X-axis guide rail. There are two first sliders, one of which is slidably connected to the upper X-axis guide rail, and the other is slidably connected to the lower X-axis guide rail. The Y-axis guide rail includes an upper Y-axis guide rail and a lower Y-axis guide rail symmetrically arranged vertically. There are two second sliders, one of which is slidably connected to the upper Y-axis guide rail, and the other is slidably connected to the lower Y-axis guide rail. The second motor is mounted on the end of the mounting base away from the connecting plate. A mounting plate is fixedly connected to the top of the Z-axis base plate, and the motors of the first and second electric lead screws are respectively mounted on the mounting plate. This design improves stability without reducing space utilization.

[0018] Due to the application of the above technical solution, this utility model has the following advantages:

[0019] This invention relates to a fully automated immunoblotting analyzer with on-demand testing. The analyzer's main structure is simple and compact, with high integration, which reduces the overall size of the analyzer and improves portability. This fully automated immunoblotting analyzer efficiently completes the complex pipetting and automatic detection processes of immunoblotting, including adding samples and reagents, diluting the secondary antibody with diluent, and transferring waste liquid, achieving true full automation. Furthermore, this fully automated immunoblotting analyzer can be used for multiple tests with on-demand testing and can also perform precision calibration of sample needles and reagent needles to ensure accurate test results. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural diagram of the fully automated immunoblotting analyzer with on-demand testing capability described in Example 1.

[0021] Figure 2 This is a three-dimensional structural diagram of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0022] Figure 3 This is a three-dimensional structural diagram of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1, viewed from another perspective.

[0023] Figure 4 This is a top view of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0024] Figure 5 This is a front view schematic diagram of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0025] Figure 6 This is a rear view of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0026] Figure 7 This is a three-dimensional structural diagram of the analyzer body of the fully automated immunoblotting analyzer with on-demand testing in Example 1, with part of the base sidewall hidden.

[0027] Figure 8 This is a front view structural diagram of the main body of the fully automated immunoblotting analyzer with on-demand testing in Example 1 (part of the base sidewall is hidden);

[0028] Figure 9 This is a right-side structural schematic diagram of the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1 (partially hidden base sidewall).

[0029] Figure 10 This is a three-dimensional structural diagram of the pipetting module, air-drying module, and image acquisition module in the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0030] Figure 11 This is a three-dimensional structural diagram of the pipetting module, air-drying module and image acquisition module in the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1, viewed from another perspective.

[0031] Figure 12 This is a three-dimensional structural diagram of the incubation reaction module (with the waste liquid pool hidden) in the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1.

[0032] Figure 13 This is a three-dimensional structural diagram of the incubation reaction module in the main body of the fully automated immunoblotting analyzer for on-demand testing in Example 1, viewed from another perspective.

[0033] Among them, 1. outer shell; 11. lid;

[0034] 2. Base; 21. Slot;

[0035] 3. Sample and reagent loading module; 31. Embedded sample holder; 32. Embedded reagent holder;

[0036] 4. Pipetting module; 411. Support base; 412. X-axis guide rail; 413. Connecting plate; 414. Side plate; 415. First synchronous belt; 416. First motor; 417. First slider; 418. Mounting base; 419. Y-axis guide rail; 420. Z-axis base plate; 421. Second synchronous belt; 422. Second motor; 423. Second slider; 424. Through groove; 425. Rotating wheel; 426. Connecting rod; 427. First Z-axis guide rail; 428. Second Z-axis guide rail; 429. Shaft guide rail; 430. Sample needle holder; 431. Reagent needle holder; 432. First electric lead screw; 433. Third slider; 434. Second electric lead screw; 435. Fourth slider; 436. Sample needle; 437. Reagent needle; 438. First plunger pump; 439. Second plunger pump; 440. Nozzle support; 441. First nozzle; 442. Second nozzle; 443. Peristaltic pump; 444. Diaphragm pump; 445. Scanning device; 446. Cleaning tank;

[0037] 5. Incubation reaction module; 51. Waste liquid tank; 52. Shaker; 53. Incubation tray; 54. Shaker motor; 55. Second through hole;

[0038] 6. Drying module; 61. Fan; 62. Robotic arm;

[0039] 7. Image acquisition module; 71. Camera; 72. Mirror; 73. Illumination device. Detailed Implementation

[0040] In the description of this utility model, it should be understood that the terms "front," "rear," "left," "right," etc., indicate the orientation or positional relationship based on the appendix. Figure 3 The orientations or positional relationships shown are for the purpose of facilitating the description of the present invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0041] In the description of this utility model, it should be understood that the term "a plurality of" includes at least two.

[0042] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "fixed," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this utility model according to the specific circumstances.

[0043] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0044] Example 1

[0045] This embodiment provides a fully automated immunoblot analyzer that allows for on-demand testing, such as... Figures 1 to 13 As shown, it includes an outer casing 1 with an openable cover 11, an analyzer body, and a control system. The outer casing 1 protects the analyzer body, and the control system controls the analyzer body to achieve fully automatic operation; both are conventional technologies in the field and will not be described in detail here.

[0046] In this embodiment, the analyzer body includes a base 2, a sample and reagent loading module 3 mounted on the base 2, a pipetting module 4, an incubation reaction module 5, an air-drying module 6, and an image acquisition module 7.

[0047] Specifically, the base 2 has a receiving cavity, a groove 21 extending from the top in a left-right direction, an incubation reaction module 5 located in front of the groove 21, and a sample and reagent loading module 3 located behind the groove 21.

[0048] Specifically, the sample and reagent loading module 3 includes an embedded sample rack 31 and an embedded reagent rack 32 mounted on the base 2. The embedded sample rack 31 has sample tube placement positions arranged in a matrix for placing sample tubes, and the embedded reagent rack 32 has reagent bottle placement positions arranged in a matrix for placing reagent bottles. In this embodiment, the embedded sample rack 31 is located in front of the embedded reagent rack 32. The embedded reagent rack 32 has reagent bottle accommodating compartments of different sizes.

[0049] Specifically, the pipetting module 4 includes a three-dimensional arm, a pipetting needle assembly, and a liquid addition / aspiration assembly. The pipetting needle assembly includes a sample needle 435 and a reagent needle 436 mounted on the three-dimensional arm. The liquid addition / aspiration assembly includes a first nozzle 440 and a second nozzle 441 mounted on the three-dimensional arm. The drying module 6 and the image acquisition module 7 are both mounted on the three-dimensional arm. The three-dimensional arm can simultaneously move the sample needle 435, the reagent needle 436, the first nozzle 440, the second nozzle 441, the drying module 6, and the image acquisition module 7 above the base 2 along the X-axis. It can simultaneously move the sample needle 435 and the reagent needle 436 above the base 2 along the Y-axis. It can also move the sample needle 435 and the reagent needle 436 above the base 2 along the Z-axis, respectively.

[0050] In this embodiment, the three-dimensional arm includes an X-axis moving support assembly, a Y-axis moving assembly, and a Z-axis moving assembly. The X-axis moving support assembly includes a support base 411 fixedly mounted in a slot 21, an X-axis guide rail 412 mounted on the support base 411, a connecting plate 413 extending along the Z-axis direction and slidably connected to the X-axis guide rail 412, a side plate 414 extending along the Y-axis direction and vertically arranged and fixedly connected to the upper end of the connecting plate 413, and a first drive assembly for driving the connecting plate 413 to move along the X-axis direction. The first drive assembly includes a first synchronous belt 415 mounted on the support base 411, a first motor 416 mounted on the support base 411 capable of driving the first synchronous belt 415, and a first slider 417 mounted on the connecting plate 413. The first slider 417 is slidably connected to the X-axis guide rail 412, and the connecting plate 413 is fixedly connected to the first synchronous belt 415. The Y-axis moving assembly includes a mounting base 418 mounted on one side surface of a side plate 414, a Y-axis guide rail 419 mounted on the mounting base 418, a Z-axis base plate 420 slidably connected to the Y-axis guide rail 419, and a second drive assembly for driving the Z-axis base plate 420 to move along the Y-axis direction. The second drive assembly includes a second synchronous belt 421 mounted on the mounting base 418, a second motor 422 mounted on the mounting base 418 capable of driving the second synchronous belt 421, and a second slider 423 mounted on the Z-axis base plate 420. The second slider 423 is slidably connected to the Y-axis guide rail 419. The Y-axis guide rail 419 is mounted on the side of the mounting base 418, and the second synchronous belt 421 is located in the mounting base 418. A through groove 424 extending along the Y-axis direction is provided on the side of the mounting base 418, and the bottom of the Z-axis base plate 420 is fixedly connected to the second synchronous belt 421 through a connecting rod 426 located in the through groove 424. The Z-axis moving assembly includes a first Z-axis guide rail 427 and a second Z-axis guide rail 428 mounted on a Z-axis base plate 420, a sample needle holder 429 slidably connected to the first Z-axis guide rail 427, a reagent needle holder 430 slidably connected to the second Z-axis guide rail 428, a third driving assembly for driving the sample needle holder 429 to move along the Z-axis direction, and a fourth driving assembly for driving the reagent needle holder 430 to move along the Z-axis direction. The third driving assembly includes a first electric lead screw 431 mounted on the Z-axis base plate 420 and a third slider 432 mounted on the sample needle holder 429 and connected to the first electric lead screw 431. The third slider 432 is slidably connected to the first Z-axis guide rail 427. The fourth driving assembly includes a second electric lead screw 433 mounted on the Z-axis base plate 420 and a fourth slider 434 mounted on the reagent needle holder 430 and connected to the second electric lead screw 433. The fourth slider 434 is slidably connected to the second Z-axis guide rail 428. Position sensors are respectively installed on the X-axis guide rail 412, Y-axis guide rail 419, first Z-axis guide rail 427, and second Z-axis guide rail 428.To improve stability, in this embodiment, the X-axis guide rail 412 includes an upper X-axis guide rail installed at the lower part of the support base 411 and a lower X-axis guide rail installed at the upper part of the support base 411. A synchronous belt is disposed between the upper and lower X-axis guide rails. There are two first sliders 417, one of which is slidably connected to the upper X-axis guide rail and the other is slidably connected to the lower X-axis guide rail. The Y-axis guide rail 419 includes an upper Y-axis guide rail and a lower Y-axis guide rail symmetrically arranged. There are two second sliders 423, one of which is slidably connected to the upper Y-axis guide rail and the other is slidably connected to the lower Y-axis guide rail. The second motor 422 is installed at the end of the mounting base 418 away from the connecting plate 413. The top of the Z-axis base plate 420 is fixedly connected to the mounting plate, and the motors of the first electric lead screw 431 and the second electric lead screw 433 are respectively installed on the mounting plate.

[0051] In this embodiment, the sample needle 435 is vertically mounted on the sample needle holder 429, and the reagent needle 436 is vertically mounted on the holder. The aspiration assembly also includes a nozzle bracket 439 rotatably connected to the lower front part of the mounting base 418. The first nozzle 440 and the second nozzle 441 are respectively fixedly mounted on the nozzle bracket 439. The pipette assembly also includes a first plunger pump 437 and a second plunger pump 438, which are connected to the sample needle 435 via a flexible tube and are mounted on the three-dimensional arm, and are connected to the reagent needle 436 via a flexible tube. The aspiration assembly also includes a peristaltic pump 442 and a diaphragm pump 443, which are connected to the first nozzle 440 via a flexible tube and are mounted in the receiving cavity, and are connected to the second nozzle 441 via a flexible tube.

[0052] In this embodiment, the Y-axis moving assembly is disposed on one side surface of the side plate 414, while the drying module 6, the image acquisition module 7, the first plunger pump 437, and the second plunger pump 438 are disposed on the other side surface of the side plate 414.

[0053] In this embodiment, a scanning device 444 and a cleaning tank 445 are installed on the connecting plate 413. The cleaning tank 445 is located in the groove 21, and the top of the cleaning tank 445 is flush with the top of the base 2. The scanning device 444 is located in the receiving cavity. The bottom of the mounting base 418 is provided with a rotating wheel 425. The lower surface plate of the rotating wheel 425 contacts the upper surface of the base 2, and the rotating shaft of the rotating wheel 425 extends along the Y-axis direction.

[0054] Specifically, the incubation reaction module 5 includes a waste liquid tank 51, a shaker 52, and multiple incubation trays 53 arranged sequentially on the shaker 52 in a left-right direction. The shaker 52 includes a bed plate mounted on the top outer surface of the base 2, a shaker motor 54 mounted on the top inner surface of the base 2, and a transmission component disposed between the bed plate and the shaker motor 54. The bed plate has multiple first through holes arranged in parallel in a left-right direction, and the base 2 has second through holes 55 at the corresponding positions of the first through holes. The first through holes and the second through holes 55 cooperate to place reagent tubes. Each incubation tray 53 includes multiple strip-shaped incubation tanks arranged in parallel in a left-right direction. Through this configuration, combined with the control program design, the fully automated immunoblotting analyzer of this invention can achieve on-demand testing and multi-item testing of a single sample. This setting allows for precision calibration of the sample needle 435 and reagent needle 436 before testing, ensuring the accuracy of the test results. Specifically, before testing, the incubation tray 53 is removed, and the control system controls the pipette assembly to add several sample or reagent needles to the reagent tube. The volume of liquid in the reagent tube is counted, and the volume of each sample or reagent needle is calculated, which is the injection volume.

[0055] Specifically, the drying module 6 includes a fan 61 and a robotic arm 62. The fan 61 is mounted at the end of the robotic arm 62, which is fixedly connected to the three-dimensional arm via a bracket. The image acquisition module 7 includes a camera 71, a reflector 72, an illumination device 73, and image acquisition and interpretation software. The camera 71, reflector 72, and illumination device 73 are fixedly connected to the three-dimensional arm via brackets. In this embodiment, the drying module 6 and the image acquisition module 7 are always located above the incubation reaction module 5.

[0056] The fully automated immunoblotting analyzer for on-demand testing in this embodiment has a simple and compact main structure with high integration, which can reduce the overall size of the immunoblotting analyzer and improve portability. This fully automated immunoblotting analyzer can efficiently complete the complex pipetting and automatic detection processes of immunoblotting, including adding samples and reagents, diluting the secondary antibody with diluent, and transferring waste liquid, thus achieving true full automation. Furthermore, this fully automated immunoblotting analyzer can be used for multiple tests and can perform on-demand testing. It can also perform precision calibration of the sample needle 435 and reagent needle 436 to ensure the accuracy of the test results.

[0057] The above embodiments are only for illustrating the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent changes or modifications made in accordance with the spirit and essence of this utility model should be included within the scope of protection of this utility model.

Claims

1. A fully automated immunoblotting analyzer for on-demand testing, comprising a housing with an openable cover, an analyzer body, and a control system, characterized in that: The analyzer body includes a base, a sample and reagent loading module mounted on the base, a pipetting module, an incubation reaction module, an air-drying module, and an image acquisition module. The base has a receiving cavity and a groove extending laterally from the top. The incubation reaction module is located in front of the groove, and the sample and reagent loading module is located behind the groove. The pipetting module includes a three-dimensional arm, a pipetting needle assembly, and a liquid aspiration assembly. The pipetting needle assembly includes a sample needle and a reagent needle mounted on the three-dimensional arm. The liquid aspiration assembly includes a first nozzle and a second nozzle mounted on the three-dimensional arm. The drying module and the image acquisition module are both mounted on the three-dimensional arm. The three-dimensional arm can simultaneously move the sample needle, reagent needle, first nozzle, second nozzle, drying module, and image acquisition module above the base along the X-axis, simultaneously move the sample needle and reagent needle above the base along the Y-axis, and separately move the sample needle and reagent needle above the base along the Z-axis.

2. The walk-away fully automated immunoblotting analyzer according to claim 1, characterized in that: The incubation reaction module includes a waste liquid tank, a shaker, and multiple incubation trays placed sequentially on the shaker in a left-right direction. The shaker includes a bed plate mounted on the top outer surface of the base, a shaker motor mounted on the top inner surface of the base, and a transmission component disposed between the bed plate and the shaker motor. The bed plate has multiple first through holes arranged side-by-side in a left-right direction. The base has second through holes corresponding to the positions of the first through holes. The first and second through holes cooperate to accommodate reagent tubes. Each incubation tray includes multiple strip-shaped incubation troughs arranged side by side along the left-right direction.

3. The walk-away fully automated immunoblotting analyzer according to claim 2, wherein, The top of the base, located in front of the groove, is an inclined surface that gradually slopes downward from the groove. Fixed seats are provided on the inclined surface on the left and right sides of the bed board of the rocker, respectively. The rear end of the bed board of the rocker is pivotally connected to the fixed seats.

4. The walk-away fully automated immunoblotting analyzer of claim 1, wherein: The air-drying module and the image acquisition module are always located above the incubation reaction module. The drying module includes a fan, a robotic arm, and a robotic arm support. The fan is mounted at the end of the robotic arm, and the robotic arm is fixedly connected to the three-dimensional arm via the support. The image acquisition module includes a camera, a reflector, an illumination device, and image acquisition and interpretation software. The camera, reflector, and illumination device are fixedly connected to the three-dimensional arm via brackets.

5. The walk-away fully automated immunoblotting analyzer of claim 1, wherein: The sample and reagent loading module includes an embedded sample rack and an embedded reagent rack mounted on the base. The embedded sample rack has sample tube placement positions arranged in a matrix for placing sample tubes, and the embedded reagent rack has reagent bottle placement positions arranged in a matrix for placing reagent bottles.

6. The walk-away fully automated immunoblotting analyzer according to any one of claims 1 to 5, characterized in that: The pipette assembly also includes a first plunger pump connected to the sample needle via a tubing and a second plunger pump connected to the reagent needle via a tubing, both mounted on the three-dimensional arm. The liquid aspiration assembly also includes a peristaltic pump connected to the first nozzle via a hose and a diaphragm pump connected to the second nozzle via a hose, both disposed in the receiving cavity.

7. The walk-away fully automated immunoblotting analyzer according to claim 6, characterized in that: The three-dimensional arm includes an X-axis moving support assembly, a Y-axis moving assembly, and a Z-axis moving assembly. The X-axis moving support assembly includes a support base fixedly installed in the groove, an X-axis guide rail installed on the support base, a connecting plate extending along the Z-axis direction and slidably connected to the X-axis guide rail, and a side plate extending along the Y-axis direction and vertically arranged and fixedly connected to the upper end of the connecting plate. The Y-axis moving assembly includes a mounting base mounted on one side surface of the side plate, a Y-axis guide rail disposed on the mounting base, and a Z-axis base plate slidably connected to the Y-axis guide rail. The Z-axis moving assembly includes a first Z-axis guide rail and a second Z-axis guide rail disposed on the Z-axis base plate, a sample needle holder slidably connected to the first Z-axis guide rail, and a reagent needle holder slidably connected to the second Z-axis guide rail.

8. The walk-away fully automated immunoblotting analyzer according to claim 7, characterized in that: The sample needle is vertically mounted on the sample needle holder, and the reagent needle is vertically mounted on the holder. The liquid aspiration assembly also includes a nozzle bracket rotatably connected to the lower front part of the mounting base, with the first nozzle and the second nozzle respectively fixedly mounted on the nozzle bracket. The Y-axis moving assembly is disposed on one side surface of the side plate, and the drying module, image acquisition module, first plunger pump, and second plunger pump are disposed on the other side surface of the side plate.

9. The walk-away fully automated immunoblotting analyzer according to claim 7, characterized in that: A scanning device and a cleaning tank are mounted on the connecting plate. The cleaning tank is located in the groove, and its top is flush with the top of the base. The scanning device is located in the receiving cavity. The mounting base has a rotating wheel at its bottom, the lower surface plate of the rotating wheel is in contact with the upper surface of the base, and the rotating shaft of the rotating wheel extends along the Y-axis.

10. The walk-away fully automated immunoblotting analyzer according to claim 7, wherein, The X-axis moving support assembly further includes a first driving assembly for driving the connecting plate to move along the X-axis direction. The first driving assembly includes a first synchronous belt mounted on the support base, a first motor mounted on the support base capable of driving the first synchronous belt to move, and a first slider mounted on the connecting plate. The first slider is slidably connected to the X-axis guide rail, and the connecting plate is fixedly connected to the first synchronous belt. The Y-axis moving assembly further includes a second driving assembly for driving the Z-axis base plate to move along the Y-axis direction. The second driving assembly includes a second synchronous belt mounted on the mounting base, a second motor mounted on the mounting base capable of driving the second synchronous belt to move, and a second slider mounted on the Z-axis base plate. The second slider is slidably connected to the Y-axis guide rail. The Y-axis guide rail is mounted on the side of the mounting base. The second synchronous belt is located in the mounting base. A through groove extending along the Y-axis direction is opened on the side of the mounting base. The bottom of the Z-axis base plate is fixedly connected to the second synchronous belt through a connecting rod located in the through groove. The Z-axis movement assembly further includes a third drive assembly for driving the sample needle holder to move along the Z-axis direction and a fourth drive assembly for driving the reagent needle holder to move along the Z-axis direction. The third drive assembly includes a first electric lead screw mounted on the Z-axis base plate and a third slider mounted on the sample needle holder and connected to the first electric lead screw. The third slider is slidably connected to the first Z-axis guide rail. The fourth drive assembly includes a second electric lead screw mounted on the Z-axis base plate and a fourth slider mounted on the reagent needle holder and connected to the second electric lead screw. The fourth slider is slidably connected to the second Z-axis guide rail. Position sensors are respectively installed on the X-axis guide rail, Y-axis guide rail, first Z-axis guide rail, and second Z-axis guide rail.

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