U-bottom microwell plate for blood group irregular antibody detection

Abstract

The utility model relates to the technical field of biological experiment equipment, and discloses a U-bottom microwell plate for blood group irregular antibody detection, which comprises a base, the inside of the base is fixedly connected with a U-bottom microwell plate, the top of the U-bottom microwell plate is fixedly connected with an anti-shaking mechanism, the inside of the base is slidably connected with a connecting mechanism, and the connecting mechanism is fixedly connected with the U-bottom microwell plate. The connecting mechanism comprises a limiting plate, the inner side of the limiting plate is fixedly connected with a plurality of first limiting rods, the interiors of the first limiting rods are fixedly connected with first springs, the other ends of the first springs are fixedly connected with limiting blocks, and a limiting assembly is arranged in the base. According to the utility model, a plurality of bases can be connected together, so that the bases can be stored tidily, and the space is saved. In the transportation process, the bases can be prevented from colliding with one another and scattering due to stable connection, the U-bottom microwell plate is protected against damage, and the safety of the U-bottom microwell plate in the transportation process is ensured.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, and in particular to a U-bottom microplate for detecting irregular blood type antibodies. Background Technology

[0002] The U-bottom microplate for blood typing irregular antibody detection is made of polystyrene and has multiple U-shaped microwells. It is used for screening irregular antibodies in blood typing, blood typing identification and antibody titer determination. It has the advantages of simple operation, accurate results, saving sample reagents and easy storage and transportation, and is widely used in the field of blood typing.

[0003] A search revealed Chinese Patent Publication No. CN204705650U, which discloses a combined microporous plate comprising a separate U-shaped microporous plate and a V-shaped microporous plate. The U-shaped microporous plate has multiple U-shaped microporous cavities, and the V-shaped microporous plate has V-shaped microporous cavities adapted to the U-shaped microporous cavities. The bottom of the V-shaped cavity protrudes from the lower surface of the V-shaped microporous plate; the portion of the V-shaped microporous cavity protruding from the lower surface of the V-shaped microporous plate has a through hole. This invention, through a novel structural design, allows the U-shaped and V-shaped microporous plates to be used in conjunction with each other. Furthermore, the U-shaped microporous plate has a retractable inclined support, enabling tilted placement of the U-shaped microporous plate, reducing the difficulty of filling, and expanding the visible range of the filling holes.

[0004] The beneficial effects mentioned in the aforementioned patent specification are as follows: "When performing non-derivative tandem mass spectrometry detection experiments of amino acids and carnitine, a blood sample is dropped onto filter paper, and a circular blood spot filter paper of a certain size is placed in the U-shaped microporous cavity 3. The inclined support 8 is pulled out to tilt the U-shaped microplate 1, and then the extraction solution is added to the U-shaped microporous cavity 3. During sample addition, the sample addition situation in the U-shaped microporous cavity 3 can be better observed. After the sample addition is completed, the inclined support 8 is retracted, and the U-shaped microplate 1 returns to a horizontal state for the next incubation step. After incubation, the U-shaped microplate is removed." Plate 1, a V-shaped microplate 2 of the same size as the U-shaped microplate 1 is placed inside the U-shaped microplate 1, and the V-shaped microwell cavity 4 is inserted into the U-shaped microwell cavity 3. Each V-shaped microwell cavity 4 has multiple through holes 5 to facilitate solution penetration. The inner wall of the V-shaped microwell cavity 4 is engraved with graduations, which can provide a reference for the amount of solution in the V-shaped microwell cavity 4. After the solution has penetrated and settled, the inserted U-shaped microplate 1 and V-shaped microplate 2 can be directly injected for analysis. During the analysis, there is no need to worry about blood stains on the filter paper affecting the analysis results. Although the above patent can provide a reference for the amount of solution in the V-shaped microwell cavity 4, it cannot connect multiple U-shaped microplates together when they need to be stored. Therefore, a U-shaped microplate for the detection of irregular antibodies in blood type is proposed to solve the above problem. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides a U-bottom microplate for detecting irregular blood type antibodies, aiming to improve the problem that the existing technology cannot achieve online monitoring to ensure the consistency and stability of processing quality.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A U-bottom microplate for detecting irregular blood type antibodies includes a base, a U-bottom microplate fixedly connected inside the base, an anti-shaking mechanism fixedly connected to the top of the U-bottom microplate, and a connecting mechanism slidably connected inside the base.

[0008] The connecting mechanism includes a limiting plate, a plurality of limiting rods are fixedly connected to the inner side of the limiting plate, a spring is fixedly connected inside the limiting rod, a limiting block is fixedly connected to the other end of the spring, and a limiting component is provided inside the base;

[0009] The above technical solution works as follows: When two bases need to be connected, pushing the limiting plate moves the inner limiting rod, compressing the spring inside the limiting rod and causing the limiting block to retract. The limiting rod is then inserted into the limiting component inside the other base. Releasing the limiting plate allows the spring to return to its original shape, pushing the limiting block out and locking it in a specific position within the limiting component, thus completing the connection between the two bases. The anti-shaking mechanism at the top of the U-bottom microplate uses elastic components, such as springs, to hold the test tube against it from both sides when it is placed, counteracting external forces and preventing the test tube from shaking inside the U-bottom microplate, ensuring stable testing operations.

[0010] As a further description of the above technical solution:

[0011] The limiting component includes a sliding groove, which is formed inside the base, and two slots are formed inside the sliding groove;

[0012] With the above technical solution: when connecting the base, the limiting plate is pushed to drive the limiting rod and the limiting block connected by the internal compression spring into the sliding groove. When the limiting block slides to the slot, the spring rebounds and pushes the limiting block into the slot, and the limiting rod is fixed, thereby realizing the two bases are stably connected by the limiting plate. The operation is convenient and the connection is firm.

[0013] As a further description of the above technical solution:

[0014] The anti-sway mechanism includes a fixed plate, the fixed plate having multiple holes inside, a second spring fixedly connected inside the holes, and a clamping plate fixedly connected to the other end of the second spring;

[0015] The above technical solution involves inserting the test tube into the hole of the fixing plate when placing it, squeezing the clamp, which causes the second spring to contract. The reaction force generated by the second spring pushes the clamp to fit tightly against the outer wall of the test tube, stabilizing the test tube from both sides, effectively offsetting the impact of external forces, preventing the test tube from shaking on the U-bottom microplate, and ensuring the stability of the sample during the detection process.

[0016] As a further description of the above technical solution:

[0017] The outer side of the limiting rod is slidably connected to the inside of the limiting assembly, and the outer side of the limiting block is slidably connected to the inside of the sliding groove;

[0018] The above technical solution involves pushing the limiting plate when connecting the base, causing the limiting rod to slide into the sliding groove. During the sliding process, the inner wall of the sliding groove presses the limiting block to retract into the limiting rod. When the limiting rod reaches the appropriate position, the limiting block aligns with the slot, and the spring pushes the limiting block to pop out and lock into the slot, thus achieving a stable connection between the two bases.

[0019] As a further description of the above technical solution:

[0020] The sliding groove is slidably connected inside the card slot, and the outer side of the limiting plate is slidably connected inside the base;

[0021] The above technical solution allows for the following: When connecting the base, the limiting plate is manually pushed to slide inside the base, causing the limiting rod to slide into the sliding groove. As it slides, the limiting block is squeezed into the inner wall of the sliding groove. When it reaches the slot position, the spring pops the limiting block out and locks it into the slot, completing the fixation and achieving a stable connection between the bases. The operation is simple and convenient.

[0022] As a further description of the above technical solution:

[0023] A limiting rod 2 is fixedly connected to the rear side of the clamping plate, and the rear side of the clamping plate is slidably connected to the outside of the fixed plate.

[0024] The above technical solution works as follows: when the test tube is placed into the U-bottom microplate, the test tube squeezes the clamping plate, and the second limiting rod on the back of the clamping plate slides on the outside of the fixed plate, while compressing the second spring. The reaction force of the second spring makes the clamping plate tightly clamp the test tube. The second limiting rod plays a guiding and limiting role, ensuring that the clamping plate applies force stably and effectively preventing the test tube from shaking.

[0025] As a further description of the above technical solution:

[0026] The outer side of the second limiting rod is slidably connected to the inside of the fixed plate, and the outer side of the limiting block is slidably connected to the inside of the first limiting rod;

[0027] Through the above technical solution: when the test tube squeezes the clamping plate, the second limiting rod on the back of the clamping plate slides in the fixed plate, and the second spring is compressed to fix the test tube. When connecting the base, the limiting plate is pushed to drive the first limiting rod, and the inner wall of the sliding groove squeezes the limiting block into the rod. After it is in place, the first spring pops out the limiting block and fixes the first limiting rod in the sliding groove, thus completing the base connection.

[0028] As a further description of the above technical solution:

[0029] The inner side of the limiting rod is provided with a sliding groove, and the inner side of the limiting plate is slidably connected to the outer side of the sliding groove;

[0030] Through the above technical solution: during the connection base operation, the limiting plate is pushed, and its inner side moves along the outer side of the sliding groove, which drives the limiting rod to move synchronously. The sliding groove inside the limiting rod provides the limiting block with a space for movement. During the sliding process, the limiting block can move in and out of the sliding groove under external pressure, so as to accurately embed into the slot and achieve a stable connection between the bases.

[0031] This utility model has the following beneficial effects:

[0032] 1. In this utility model, a limiting plate is used in conjunction with a spring, which in turn works with a limiting block. The limiting block then works with a slot, which in turn works with a sliding groove, thereby achieving the connection between two bases. When not in use, multiple bases can be connected together for neat storage, saving space. During transportation, the stable connection also prevents the bases from colliding and falling apart, protecting the U-shaped microporous plate from damage and ensuring its safety during transport.

[0033] 2. In this utility model, the fixed plate works in conjunction with the second spring, and the second spring works in conjunction with the clamping plate to prevent the test tube placed inside the U-bottom microplate from shaking unexpectedly. In the process of irregular antibody detection of blood type, the stability of the test tube is crucial. Shaking will cause the blood sample in the test tube to vibrate, affecting the reaction process of red blood cells and antibodies and the observation of results. It may destroy the agglutination phenomenon that has already formed, or cause false negative results for samples that have not fully reacted. Stable test tube placement can ensure the accuracy and reliability of the test results. Attached Figure Description

[0034] Figure 1 This is a three-dimensional schematic diagram of a U-bottom microplate for detecting irregular antibodies in blood types, as proposed in this utility model.

[0035] Figure 2 This is a schematic diagram of the limiting plate of a U-bottom microplate for detecting irregular antibodies in blood types, as proposed in this utility model.

[0036] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0037] Figure 4 for Figure 2 Enlarged view of point B in the middle.

[0038] Legend:

[0039] 1. Base; 2. U-shaped micro-perforated plate; 3. Connecting mechanism; 301. Limiting plate; 302. Limiting rod one; 303. Limiting block; 304. Spring one; 305. Limiting component; 3051. Sliding groove; 3052. Slot; 4. Anti-shaking mechanism; 401. Fixing plate; 402. Spring two; 403. Clamping plate; 404. Limiting rod two; 405. Hole. Detailed Implementation

[0040] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0041] Reference Figures 1 to 3This utility model provides an embodiment of a U-bottom microplate for detecting irregular blood type antibodies, including a base 1, which serves as the basic support structure for the entire device, providing a stable mounting platform to ensure that it will not easily shake during operation and guaranteeing the smooth progress of the detection work. A U-bottom microplate 2 is fixedly connected inside the base 1. The unique U-shaped bottom design of the U-bottom microplate 2 facilitates the distribution and observation of red blood cells within the wells, clearly presenting the agglutination reaction results and greatly improving the accuracy and efficiency of the detection. An anti-shake mechanism 4 is fixedly connected to the top of the U-bottom microplate 2, effectively preventing it from being placed on the U-bottom. In case of accidental shaking of the test tubes inside the microplate 2, the stability of the samples inside the test tubes is ensured, thereby reducing detection errors caused by shaking. A connecting mechanism 3 is slidably connected inside the base 1. The connecting mechanism 3 allows for convenient and quick connection between two bases 1, enabling the device to be flexibly combined and expanded according to actual needs. The connecting mechanism 3 includes a limiting plate 301, the outer side of which is slidably connected to the inside of the base 1. This design allows the limiting plate 301 to slide smoothly within the base 1, providing a smooth motion basis for subsequent connection operations. The outer side of the limiting plate 301 is slidably connected to the inside of the base 1, and the inner side of the limiting plate 301... Multiple limiting rods 302 are fixedly connected to the side. Each limiting rod 302 has a groove inside, providing space for the sliding of the limiting block 303, ensuring that the limiting block 303 can move flexibly within the limiting rod 302. A spring 304 is fixedly connected inside the limiting rod 302. The spring 304 has elastic restoring force, which can push the limiting block 303 out during connection, achieving a stable connection. The other end of the spring 304 is fixedly connected to the limiting block 303, which can slide within the limiting rod 302. When it reaches the appropriate position, it can accurately engage with the corresponding slot 3052, completing the connection of the base 1. Next, the outer side of the limiting block 303 is slidably connected to the inside of the limiting rod 302. This sliding connection ensures the smooth movement of the limiting block 303 within the limiting rod 302 and improves the reliability of the connection operation. The base 1 has a limiting component 305 inside. The limiting component 305 provides guidance and fixation for the sliding and connection of the limiting rod 302, ensuring the accuracy and stability of the connection process. The outer side of the limiting rod 302 is slidably connected to the inside of the limiting component 305, so that the limiting rod 302 can slide accurately along the limiting component 305 and smoothly cooperate with the slot 3052 to complete the connection of the base 1.

[0042] Specifically, the device mainly consists of a base 1, a U-shaped microplate 2, an anti-shake mechanism 4, and a connecting mechanism 3. The base 1 provides a stable platform for the components. The U-shaped microplate 2 is fixed inside the base 1, and its U-shaped bottom facilitates the observation of red blood cell distribution, improving detection accuracy and efficiency. The anti-shake mechanism 4 is located on top of the U-shaped microplate 2, preventing the test tube from shaking, ensuring sample stability, and reducing detection errors. The connecting mechanism 3 is inside the base 1 and includes a limiting plate 301, a limiting rod 302, a spring 304, a limiting block 303, and a limiting component 305. The limiting plate 301 slides inside the base 1, driving the limiting rod 302. The spring 304 pushes the limiting block 303 to slide inside the limiting rod 302, engaging in the slot 3052 of the limiting component 305, thus achieving flexible connection and device expansion between the bases 1, ensuring accurate, stable, and reliable connection.

[0043] The limiting component 305 includes a sliding groove 3051, which provides a precise track for the sliding of the limiting plate 301 and the limiting rod 302, ensuring that the connection operation proceeds along a predetermined path and greatly improving the stability and reliability of the connection. The inner side of the limiting plate 301 is slidably connected to the outer side of the sliding groove 3051. This design allows the limiting plate 301 to slide smoothly on the outer side of the sliding groove 3051, making it convenient for the operator to push the limiting plate 301 to move the limiting rod 302 and realize the connection preparation action between the bases 1. The outer side of the limiting block 303 is slidably connected to the inside of the sliding groove 3051. The sliding groove 3051 provides space for the sliding of the limiting block 303. During the connection process, the limiting block 303 can move flexibly in and out of the sliding groove 3051. When it reaches a specific position, it can quickly engage with the slot 305. 2. The connection is completed. The sliding groove 3051 is opened inside the base 1. The ingenious built-in design not only saves space, but also makes the entire connection mechanism 3 more compact. At the same time, it avoids external factors from interfering with the connection operation and ensures the stability of the device. The sliding groove 3051 has two slots 3052 inside. The setting of the two slots 3052 increases the firmness of the connection. The limiting block 303 can be accurately locked into the slot 3052 to prevent the limiting rod 302 from sliding accidentally after connection, ensuring that the two bases 1 are tightly connected. The limiting rod 302 slides in the slot 3052. When the limiting rod 302 slides into the slot 3052, the initial connection of the two bases 1 is achieved. The slot 3052 tightly locks the limiting rod 302, effectively preventing the connection part from loosening and ensuring the stability of the device during use.

[0044] Specifically, the limiting component 305 includes a sliding groove 3051 and a slot 3052. The sliding groove 3051 provides a track and space for the sliding of the limiting plate 301, the limiting rod 302, and the limiting block 303, so that the connection operation is carried out according to a predetermined path. The built-in design saves space and avoids interference. The limiting plate 301 can slide along the outside of the sliding groove 3051 to drive the limiting rod 302. The limiting block 303 can move flexibly in and out of the groove. The two slots 3052 in the sliding groove 3051 increase the connection firmness. The limiting rod 302 slides into the slot 3052 to achieve the initial connection of the base 1, prevent the connection part from loosening, and ensure the stability of the device during use.

[0045] Reference Figure 1 , Figure 2 and Figure 4 The anti-sway mechanism 4 includes a fixing plate 401. The fixing plate 401 serves as the basic structure of the entire anti-sway mechanism 4, providing stable installation support for the components and ensuring effective anti-sway function during subsequent use. The fixing plate 401 has multiple holes 405 inside. These holes 405 are precisely designed to fit the size of the test tube, facilitating insertion. They also provide space for the installation and operation of the second spring 402 and the clamping plate 403. The second spring 402 is fixedly connected inside the holes 405. The second spring 402 has good elasticity; when the test tube is inserted into the hole 405 and presses against the clamping plate 403, the second spring 402 is compressed, generating a reaction force that tightly holds the clamping plate 403, providing a stable clamping force for the test tube. The other end of the second spring 402 is fixedly connected to the clamping plate 403, which directly contacts the test tube. Under the elastic force of the second spring 402, the clamping plate 403 can tightly clamp the test tube from both sides, effectively counteracting external forces that could cause the test tube to shake, ensuring the test tube remains within the micro-hole at the bottom of the U-shaped opening. To ensure stability within plate 2, the rear side of clamping plate 403 is slidably connected to the outside of fixed plate 401. This sliding connection allows clamping plate 403 to move flexibly outside of fixed plate 401 when squeezed by test tube, thus smoothly compressing spring 402. Simultaneously, when spring 402 rebounds, it can stably apply clamping force to the test tube. A limiting rod 404 is fixedly connected to the rear side of clamping plate 403. The limiting rod 404 guides and limits the sliding of clamping plate 403, ensuring that clamping plate 403 maintains a stable posture during sliding, accurately clamping the test tube and preventing the anti-shaking effect from being affected by clamping plate 403 shaking or shifting. The outer side of limiting rod 404 is slidably connected to the inside of fixed plate 401. Fixed plate 401 provides a precise track for the sliding of limiting rod 404, ensuring that limiting rod 404 does not deviate during sliding, thereby ensuring that clamping plate 403 can stably apply clamping force to the test tube and continuously perform its anti-shaking function.

[0046] Specifically, the core of the anti-sway mechanism 4 is the fixed plate 401, which supports the entire anti-sway system. The fixed plate 401 has holes 405 that are adapted to the size of the test tube, which facilitates the insertion of the test tube and provides operating space for the second spring 402 and the clamping plate 403. When the test tube is inserted, it squeezes the clamping plate 403 and compresses the second spring 402. The reaction force of the second spring 402 pushes the clamping plate 403 to tightly clamp the test tube and counteract the shaking force. The limiting rod 404 on the rear side of the clamping plate 403 slides in the fixed plate 401 to guide and limit the clamping plate 403, ensuring that it stably clamps the test tube and continuously plays the anti-sway role, ensuring the stability of the test tube in the U-bottom microporous plate 2.

[0047] Working principle: When medical staff need to use the base 1, they can directly place the test tube into the U-bottom microplate 2. When the test tube is placed into the U-bottom microplate 2, the clamps 403 on both sides of the test tube will squeeze it to the sides. After the test tube is placed into the U-bottom microplate 2, the compressed spring 402 will drive the clamps 403 to push the outside of the test tube, thereby preventing the test tube from shaking unexpectedly after being placed into the U-bottom microplate 2.

[0048] When it is necessary to connect two bases 1, the two bases 1 can be placed together, and then multiple limiting rods 302 can be moved by manually controlling the air through the limiting plate 301. At this time, the limiting rods 302 slide into the sliding groove 3051. There are two limiting blocks 303 sliding inside the limiting rods 302. When the limiting blocks 303 slide into the sliding groove 3051, they will be squeezed into the limiting rods 302 by the inner wall of the sliding groove 3051. There are two slots 3052 inside the sliding groove 3051. When the limiting blocks 303 slide to the outside of the slots 3052, the limiting blocks 303 will be pushed into the slots 3052 by the compressed spring 304 because there is room for sliding on the outside. This restricts the limiting rods 302 inside the sliding groove 3051. At this time, the two bases 1 can be connected together by the limiting plate 301.

[0049] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A U-bottom microwell plate for detecting blood group irregular antibodies, comprising a base (1), characterized in that: The base (1) is fixedly connected to a U-shaped microperforated plate (2), the top of the U-shaped microperforated plate (2) is fixedly connected to an anti-shaking mechanism (4), and the base (1) is slidably connected to a connecting mechanism (3). The connecting mechanism (3) includes a limiting plate (301), a plurality of limiting rods (302) are fixedly connected to the inner side of the limiting plate (301), a spring (304) is fixedly connected inside the limiting rod (302), a limiting block (303) is fixedly connected to the other end of the spring (304), and a limiting component (305) is provided inside the base (1).

2. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 1, characterized in that: The limiting component (305) includes a sliding groove (3051) which is formed inside the base (1), and two slots (3052) are formed inside the sliding groove (3051).

3. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 1, characterized in that: The anti-sway mechanism (4) includes a fixed plate (401), and the fixed plate (401) has multiple holes (405) inside. A second spring (402) is fixedly connected inside the holes (405), and a clamping plate (403) is fixedly connected to the other end of the second spring (402).

4. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 2, characterized in that: The outer side of the limiting rod (302) is slidably connected to the inside of the limiting assembly (305), and the outer side of the limiting block (303) is slidably connected to the inside of the sliding groove (3051).

5. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 2, characterized in that: The limiting rod (302) is slidably connected inside the slot (3052), and the outer side of the limiting plate (301) is slidably connected inside the base (1).

6. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 3, characterized in that: The rear side of the clamping plate (403) is fixedly connected to a limiting rod (404), and the rear side of the clamping plate (403) is slidably connected to the outside of the fixed plate (401).

7. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 6, characterized in that: The outer side of the second limiting rod (404) is slidably connected to the inside of the fixed plate (401), and the outer side of the limiting block (303) is slidably connected to the inside of the first limiting rod (302).

8. The U-bottom microwell plate for detecting blood group irregular antibody according to claim 2, characterized in that: The inner side of the limiting rod (302) is provided with a sliding groove, and the inner side of the limiting plate (301) is slidably connected to the outer side of the sliding groove (3051).

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