Reagent cavity structure and microfluidic detection chip
By designing a connection structure between the reagent chamber and the buffer chamber in a microfluidic chip, and using magnetic components to drag and transfer reagents, the problems of reagent mixing and dead volume are solved, thereby improving the purity and detection accuracy of nucleic acid elution products.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU BAOCHUANG BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-05-15
- Publication Date
- 2026-04-28
AI Technical Summary
In existing microfluidic chips, different reagents are easily mixed with each other, and a large amount of dead volume exists during the transfer process, which affects the accuracy of detection.
Design a reagent chamber structure including a substrate and a sealing layer. The reagent chamber and the buffer chamber are connected by a drag channel. The minimum height of the drag channel is greater than the bottom wall height of the reagent chamber. The liquid in the reagent chamber can enter the adjacent buffer chamber to avoid mixing, and the reagent is transferred by dragging the magnetic component.
It reduces the dead volume during nucleic acid extraction, improves the purity of nucleic acid elution products, avoids cross-contamination between reagents, and improves the accuracy of detection.
Smart Images
Figure CN224167541U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of microfluidic chip technology, and in particular to a reagent chamber structure and a microfluidic detection chip. Background Technology
[0002] In related technologies, microfluidic chips, also known as lab-on-a-chip technologies, are... Microfluidic chip technology offers advantages such as small sample volume, rapid analysis speed, ease of manufacturing into portable instruments, and suitability for point-of-care and on-site analysis, and has been widely applied in many fields including biology, chemistry, and medicine. The combination of microfluidic chips with in vitro diagnostic reagents and technologies has led to the development of numerous rapid detection technologies, such as microfluidic-based colloidal gold immunoassay, immunofluorescence assay, chemiluminescence assay, and microfluidic-based nucleic acid assay.
[0003] In existing microfluidic chips, different reagents located in different reagent chambers are prone to mixing. Furthermore, in some microfluidic chips, different reaction reagents may need to be transferred in the same or different reagent chambers. During the transfer process, a large amount of dead volume exists, causing cross-contamination between reagents and affecting the accuracy of detection. Utility Model Content
[0004] This application aims to address at least one of the technical problems existing in the prior art. To this end, this application proposes a reagent chamber structure and a microfluidic detection chip that can improve the purity of elution products.
[0005] An embodiment of the first aspect of this application provides a reagent chamber structure, including:
[0006] A substrate having a first end face, on which a reagent cavity and a buffer cavity are provided, wherein the reagent cavities are multiple and spaced apart, and at least one buffer cavity is provided between two adjacent reagent cavities, and the buffer cavity is connected to the adjacent reagent cavity through a drag channel, wherein the minimum height of the drag channel is greater than the height of the bottom wall of the reagent cavity.
[0007] The first sealing layer is disposed on the first end face.
[0008] Furthermore, the number of reagent chambers is at least three.
[0009] Furthermore, the number of reagent chambers is 5.
[0010] Furthermore, the multiple reagent chambers are arranged sequentially along the same straight line.
[0011] Furthermore, the reagent chamber includes a first reagent chamber, which is provided with spaced liquid retardation structures. Along the flow direction of the liquid reagent, each of the liquid retardation structures gradually moves closer to the extension line of the dragging channel.
[0012] Furthermore, the reagent chamber includes a fourth reagent chamber and a fifth reagent chamber, and two buffer chambers are provided between the fourth reagent chamber and the fifth reagent chamber.
[0013] Furthermore, each of the reagent chambers and the adjacent buffer chambers has a separation structure, and the drag channel is formed on top of the separation structure.
[0014] Furthermore, the partition structure between the reagent chamber and the previous buffer chamber is arc-shaped when projected in a direction perpendicular to the first end face, and both ends of the partition structure are bent toward the reagent chamber.
[0015] Furthermore, the bottom wall of the drag channel smoothly transitions to the side wall of the partition structure.
[0016] An embodiment of the second aspect of this application provides a microfluidic detection chip, including the reagent chamber structure as described above.
[0017] As can be seen from the above technical solutions, the embodiments of this application have at least the following beneficial effects:
[0018] In the reagent chamber structure and microfluidic detection chip provided in this application embodiment, substances such as lysis binding solution and washing solution that have already been transferred into the reagent chamber do not need to be transferred across chambers. Therefore, no waste liquid is generated, and there is no need to set up a waste liquid chamber on the microfluidic chip. The dead volume during nucleic acid extraction is small, and the purity of nucleic acid elution products is high. At the same time, liquid reagents overflowing from the reagent chamber can enter adjacent buffer chambers, avoiding the mixing and contamination of liquid reagents in different reagent chambers, which is beneficial to improving the purity of nucleic acid elution products. Attached Figure Description
[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0020] Figure 1 This is a schematic diagram of the structure of a microfluidic detection chip provided in one embodiment of this application;
[0021] Figure 2 This is a top view schematic diagram of a microfluidic detection chip provided in one embodiment of this application;
[0022] Figure 3 for Figure 2 A magnified schematic diagram of part A in the middle section;
[0023] Figure 4 for Figure 2 A magnified schematic diagram of part B in the middle section.
[0024] Figure label:
[0025] 100, substrate; 110, reagent chamber; 111, first reagent chamber; 1111, liquid inlet; 112, second reagent chamber; 113, third reagent chamber; 114, fourth reagent chamber; 115, fifth reagent chamber; 120, buffer chamber; 131, first liquid hysteresis structure; 132, second liquid hysteresis structure; 140, partition structure; 141, drag channel. Detailed Implementation
[0026] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0027] See Figures 1 to 4 As shown, an embodiment of the first aspect of this application discloses a reagent chamber structure, including a substrate 100 and a first sealing layer.
[0028] Specifically, the substrate 100 has a first end face, on which a reagent cavity 110 and a buffer cavity 120 are provided. The reagent cavities 110 are multiple and spaced apart. At least one buffer cavity 120 is provided between two adjacent reagent cavities 110. The buffer cavity 120 is connected to the adjacent reagent cavity 110 through a drag channel 141. The lowest height of the drag channel 141 is greater than the height of the bottom wall of the reagent cavity 110. A first sealing layer is provided on the first end face to seal each reagent cavity 110 and the buffer cavity 120.
[0029] In the reagent chamber structure provided in this embodiment, during the nucleic acid extraction process, substances such as lysis binding solution and washing solution that have already been transferred into the reagent chamber 110 do not need to be transferred across chambers. Therefore, no waste liquid is generated, and there is no need to set up a waste liquid chamber on the microfluidic chip. The dead volume during the nucleic acid extraction process is small, and the purity of the nucleic acid elution product is high. At the same time, liquid reagents overflowing from the reagent chamber 110 can enter the adjacent buffer chamber 120, avoiding the mixing of liquid reagents in different reagent chambers 110, which also helps to improve the purity of the nucleic acid elution product.
[0030] In some embodiments of this application, the number of reagent chambers 110 is at least three. It is understood that the number of reagent chambers 110 can be set according to reagent preparation needs.
[0031] In one possible implementation, there are five reagent chambers 110, which can be used to hold different or the same liquid reagents as needed.
[0032] In one possible implementation, see Figure 1 Multiple reagent chambers 110 are arranged sequentially along the same straight line. This allows for convenient transfer of the elution product between different reagent chambers 110.
[0033] In one possible implementation, see Figure 1 and Figure 2 As shown, there are five reagent chambers 110, which are arranged sequentially along the same straight line. The five reagent chambers 110 are, in order, the first reagent chamber 111, the second reagent chamber 112, the third reagent chamber 113, the fourth reagent chamber 114, and the fifth reagent chamber 115.
[0034] In practical applications, magnetic particles enter the first reagent chamber 111. Magnetic components (such as magnets) are placed at the top and bottom of the first reagent chamber 111. By driving the magnetic components close to the substrate 100 and then dragging them laterally, the magnetic particles are transferred through the dragging channel 141 to the buffer chamber 120 between the first reagent chamber 111 and the second reagent chamber 112. Dragging continues, transferring the magnetic particles from the buffer chamber 120 to the second reagent chamber 112, and so on. Thus, the magnetic particles can sequentially pass through the first reagent chamber 111, the second reagent chamber 112, the third reagent chamber 113, the fourth reagent chamber 114, and the fifth reagent chamber 115.
[0035] In the above embodiments, the buffer chamber 120 includes a first buffer chamber, a second buffer chamber, a third buffer chamber, and a fourth buffer chamber. Specifically, the buffer chamber 120 between the first reagent chamber 111 and the second reagent chamber 112 is designated as the first buffer chamber; the buffer chamber 120 between the second reagent chamber 112 and the third reagent chamber 113 is designated as the second buffer chamber; the buffer chamber 120 between the third reagent chamber 113 and the fourth reagent chamber 114 is designated as the third buffer chamber; and the buffer chamber 120 between the fourth reagent chamber 114 and the fifth reagent chamber 115 is designated as the fourth buffer chamber.
[0036] It is worth understanding that the number of buffer chambers 120 located between two adjacent reagent chambers 110 can be set to one or more as needed, where more includes two.
[0037] In some embodiments of this application, the reagent chamber 110 includes a fourth reagent chamber 114 and a fifth reagent chamber 115, with two buffer chambers 120 disposed between the fourth reagent chamber 114 and the fifth reagent chamber 115. This effectively prevents the liquid reagent in the fourth reagent chamber 114 from mixing with the reagent in the fifth reagent chamber 115, thus helping to ensure the purity of the elution product.
[0038] In some embodiments of this application, see Figure 2 and Figure 3 As shown, the reagent chamber 110 includes a first reagent chamber 111, which contains a plurality of spaced-apart liquid retardation structures along the flow direction of the liquid reagent (i.e., Figure 2 In the X direction, each liquid hysteresis structure gradually approaches the extension line of the drag channel 141. Each liquid hysteresis structure slows down the flow rate of the liquid reagent in the first reagent chamber 111 to prevent the liquid reagent from directly flowing into the next reagent chamber 110. This reduces the probability of the reagent in the first reagent chamber 111 mixing with the reagent in the next reagent chamber 110 (i.e., the second reagent chamber 112).
[0039] In the above embodiments, see Figure 2 The liquid inlet 1111 of the first reagent chamber 111 is located at the end of the first reagent chamber 111 away from the buffer chamber 120, and is located on the side of each liquid hysteresis structure away from the buffer chamber 120.
[0040] In this embodiment, see Figure 2 and Figure 3 The liquid hysteresis structure includes a first liquid hysteresis structure 131 and a second liquid hysteresis structure 132 spaced apart along the X direction. The second liquid hysteresis structure 132 is positioned closer to the extension line of the drag channel 141 than the first liquid hysteresis structure 131. In this way, the flow rate of the liquid reagent in the first reagent chamber 111 can be gradually reduced.
[0041] In some embodiments of this application, see Figure 2 and Figure 3 Each reagent chamber 110 and the adjacent buffer chamber 120 are separated by a partition structure 140, and a drag channel 141 is formed on the top of the partition structure 140. The reagent chamber 110 is on one side of the partition structure 140, and the buffer chamber 120 is on the other side.
[0042] In this embodiment, the drag channel 141 is connected to the side of the first sealing layer near the reagent chamber 110. Therefore, when the magnetic particles move along the side of the first sealing layer near the reagent chamber, they can pass directly through the drag channel 141.
[0043] In some embodiments of this application, the projection of the partition structure 140 between the reagent chamber 110 and the preceding buffer chamber 120 in the direction perpendicular to the first end face is arc-shaped, and both ends of the partition structure 140 are bent toward the reagent chamber 110. The bending of both ends of the partition structure 140 toward the reagent chamber 110 allows the liquid reagent in the reagent chamber 110 to quickly fill the chamber, ensuring that the magnetic particles can fully contact the liquid reagent in the reagent chamber 110.
[0044] Furthermore, the drag channel 141 is located at the middle of the top of the partition structure 140, which helps to reduce the longitudinal movement distance of the magnetic particles within the reagent chamber 110 and facilitates the transfer of magnetic particles between the various reagent chambers 110. It is worth understanding that longitudinal refers to the direction perpendicular to the X-direction.
[0045] In some embodiments of this application, the bottom wall of the drag channel 141 smoothly transitions to the side wall of the partition structure 140 to facilitate the magnetic particles crossing the partition structure 140.
[0046] An embodiment of the second aspect of this application discloses a microfluidic detection chip, including the reagent chamber structure as described above, and having all the technical effects of the reagent chamber structure as described above.
[0047] For example, in the microfluidic detection chip provided in this application embodiment, substances such as lysis binding solution and washing solution that have already been transferred to the reagent chamber 110 do not need to be transferred across chambers. Therefore, no waste liquid is generated, and there is no need to set up a waste liquid chamber on the microfluidic chip. The dead volume during nucleic acid extraction is small, and the purity of nucleic acid elution products is high. At the same time, liquid reagents overflowing from the reagent chamber 110 can enter the adjacent buffer chamber 120, avoiding the mixing of liquid reagents in different reagent chambers 110, which also helps to improve the purity of nucleic acid elution products.
[0048] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0049] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.
[0050] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0051] In the description of this specification, specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples.
[0052] It should be understood that although the steps in the flowcharts of the accompanying figures are shown sequentially as indicated by the arrows, these steps are not necessarily executed in the order indicated by the arrows. Unless explicitly stated herein, there is no strict order restriction on the execution of these steps, and they can be executed in other orders. Moreover, at least some steps in the flowcharts of the accompanying figures may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily completed at the same time, but can be executed at different times, and their execution order is not necessarily sequential, but can be performed alternately or in turn with other steps or at least some of the sub-steps or stages of other steps.
Claims
1. A reagent chamber structure, characterized in that, include: A substrate having a first end face, on which a reagent cavity and a buffer cavity are provided, wherein the reagent cavities are multiple and spaced apart, and at least one buffer cavity is provided between two adjacent reagent cavities, and the buffer cavity is connected to the adjacent reagent cavity through a drag channel, wherein the minimum height of the drag channel is greater than the height of the bottom wall of the reagent cavity; The first sealing layer is disposed on the first end face.
2. The reagent chamber structure according to claim 1, characterized in that, The number of reagent chambers is at least three.
3. The reagent chamber structure according to claim 2, characterized in that, The reagent chambers consist of 5 cells.
4. The reagent chamber structure according to any one of claims 1 to 3, characterized in that, The reagent chambers are arranged sequentially along the same straight line.
5. The reagent chamber structure according to claim 4, characterized in that, The reagent chamber includes a first reagent chamber, which is provided with spaced liquid retardation structures. Along the flow direction of the liquid reagent, each liquid retardation structure gradually moves closer to the extension line of the dragging channel.
6. The reagent chamber structure according to claim 3, characterized in that, The reagent chamber includes a fourth reagent chamber and a fifth reagent chamber, and two buffer chambers are provided between the fourth reagent chamber and the fifth reagent chamber.
7. The reagent chamber structure according to claim 1, characterized in that, Each of the reagent chambers and the adjacent buffer chambers are separated by a partition structure, and the drag channel is formed on top of the partition structure.
8. The reagent chamber structure according to claim 7, characterized in that, The partition structure between the reagent chamber and the previous buffer chamber is arc-shaped when projected in a direction perpendicular to the first end face, and both ends of the partition structure are bent toward the reagent chamber.
9. The reagent chamber structure according to claim 7, characterized in that, The bottom wall of the drag channel smoothly transitions to the side wall of the partition structure.
10. A microfluidic detection chip, characterized in that, Includes the reagent chamber structure as described in any one of claims 1 to 9.