Integrated pipetting device based on cell slide-making dyeing machine

Abstract

The utility model relates to the technical field of cell pipetting, and discloses an integrated pipetting device based on a cell slide-making dyeing machine, which comprises a rack, grooves I are formed in two sides of the inner wall of the rack, ultraviolet tubes are fixedly connected to the inner walls of the two grooves I, a thermometer is fixedly connected to the left side of the inner wall of the rack, and a temperature sensor is fixedly connected to the left side of the inner wall of the rack. A control line is fixedly connected to the outer wall of the thermometer, a heating box is fixedly connected to the bottom of the rear side of the control line, a first motor is fixedly connected to the bottom of the inner wall of the heating box, and a heating wire is fixedly connected to the middle of the inner wall of the heating box. According to the utility model, the interior of the equipment is disinfected through the ultraviolet lamp to create a sterile environment, the middle part of the inner wall of the heating box is fixedly connected with the heating wire which can dissipate heat, the fan blows out hot air, the middle part of the inner wall of the refrigeration box is fixedly connected with the refrigeration sheet, and the rear side of the outer wall of the refrigeration sheet is communicated with the air outlet which can blow out cold air.

Description

Technical Field

[0001] This utility model relates to the field of cell pipetting technology, and in particular to an integrated pipetting device based on a cell slide staining machine. Background Technology

[0002] With the continuous advancement of medical technology, cytopathological testing plays an increasingly important role in disease diagnosis. Liquid-based thin-layer cytology (LTB) is one of the commonly used detection methods, including membrane methods, natural sedimentation methods, and centrifugation methods. Among them, sedimentation-based LTB is widely used due to its stable slide quality. However, this method has a complex process, involving multiple steps such as sample cell collection, mixing, multiple gradient centrifugation, adding buffer solution, transferring cells to glass slides for natural sedimentation, and subsequent staining. Each step has a significant impact on cell morphology and detection results, which requires precise and efficient pipetting devices to ensure the accuracy and consistency of liquid handling in each step.

[0003] In traditional cell preparation and staining processes, specimens or reagents are often added manually using disposable syringes. This method is not only inefficient but also prone to human error. Previously, long liquid channels connecting the air pump and the staining container were commonly used for staining transfer. However, these long channels present several problems, such as large volumes of liquid and inconsistent internal temperatures affecting cell viability. Current market solutions utilize technologies like motor drives, mechanical transmissions, and microcontrollers to precisely control the movement of the pipette tip and the liquid aspiration and dispensing process. Redesigning the liquid channel layout to place the pipette container closer to the work area and reduce the length of the liquid channels has solved the infection problem associated with excessively long pipettes. However, the internal temperature control of most pipettes remains unresolved. A suitable temperature environment is beneficial for maintaining cell viability and significantly improving cell survival rates. Therefore, an integrated pipetting device for cell preparation and staining machines with adjustable temperature is needed. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides an integrated pipetting device based on a cell slide staining machine, which aims to improve the problem that the internal temperature of the equipment in the prior art cannot be adjusted.

[0005] To achieve the above objectives, this utility model adopts the following technical solution: an integrated pipetting device based on a cell slide staining machine, comprising a frame, with grooves on both sides of the inner wall of the frame, ultraviolet tubes fixedly connected to the inner walls of both grooves, a thermometer fixedly connected to the left side of the inner wall of the frame, a control line fixedly connected to the outer wall of the thermometer, a heating box fixedly connected to the bottom rear side of the control line, a motor fixedly connected to the bottom inner wall of the heating box, a fan fixedly connected to the output end of the motor, a heating wire fixedly connected to the middle of the inner wall of the heating box, a dispersion plate fixedly connected to the front side of the inner wall of the heating box, a cooling box fixedly connected to the top outer wall of the control line, a cooling plate fixedly connected to the bottom inner wall of the cooling box, an air outlet connected to the bottom outer wall of the cooling plate, a dispersion plate fixedly connected to the front side of the inner wall of the cooling box, and a uniform mixing mechanism provided in the middle inner side of the frame for uniform mixing of the solution.

[0006] As a further description of the above technical solution:

[0007] The uniform mixing mechanism includes a fixed block, the left side of the outer wall of the fixed block is slidably connected to the right side of the inner wall of the frame, a fixed box is fixedly connected to the rear side of the outer wall of the fixed block, a second motor is fixedly connected to the top of the inner wall of the fixed box, a vibrating wheel is fixedly connected to the output end of the second motor, a vibrating plate is fixedly connected to the rear bottom side of the fixed block, a second groove is provided on the rear side of the outer wall of the vibrating plate, a conductive plate is fixedly connected to the rear side of the outer wall of the vibrating plate, a pipette is fixedly connected to the bottom of the outer wall of the fixed block, and a damping ring is fixedly connected to the outer wall of the pipette.

[0008] As a further description of the above technical solution:

[0009] A support column is fixedly connected to the left side of the outer wall of the frame, and an electric motor is fixedly connected to the top of the support column.

[0010] As a further description of the above technical solution:

[0011] The output end of the motor is fixedly connected to a second transmission ring, and a conveyor belt is driven to the middle of the outer wall of the second transmission ring. A first transmission ring is driven to the left side of the conveyor belt.

[0012] As a further description of the above technical solution:

[0013] A threaded rod is fixedly connected to the bottom of the transmission ring, and a threaded sleeve is threadedly connected to the outer wall of the threaded rod.

[0014] As a further description of the above technical solution:

[0015] The outer wall of the threaded sleeve is threadedly connected to a movable column, and a fixed plate is fixedly connected to the middle of the outer wall of the movable column.

[0016] As a further description of the above technical solution:

[0017] A support block is fixedly connected to the top of the frame, and a base plate is fixedly connected to the bottom of the frame.

[0018] As a further description of the above technical solution:

[0019] A groove is provided on the top of the outer wall of the base plate, and a sliding strip is fixedly connected to the middle of the inner wall of the groove. A dyeing machine is slidably connected to the middle of the outer wall of the base plate.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, an ultraviolet lamp is used to sterilize the inside of the equipment to create a sterile environment. At the same time, a heating wire is fixedly connected to the middle of the inner wall of the heating box to dissipate heat, and a fan blows out hot air. A cooling plate is fixedly connected to the middle of the inner wall of the cooling box, and an air outlet is connected to the rear side of the outer wall of the cooling plate to blow out cold air. In this way, the temperature inside the equipment is regulated, providing cells with a suitable environment.

[0022] 2. In this utility model, a vibrating wheel is fixedly connected to the output end of motor two, which drives the vibrating wheel to move. At the same time, the vibrating wheel hits the vibrating plate to generate vibration. A conduction plate is fixedly connected to the front side of the outer wall of the vibrating plate, and damping rings are fixedly connected to both sides of the conduction plate to play a damping role. A pipette is fixedly connected to the inner wall of the damping ring, thus realizing the uniform mixing of the solution. Attached Figure Description

[0023] Figure 1 This is a front perspective view of the integrated pipetting device based on a cell slide staining machine proposed in this utility model;

[0024] Figure 2 This is a partial structural diagram of the integrated pipetting device based on a cell slide staining machine proposed in this utility model;

[0025] Figure 3 This is a partial structural diagram of the integrated pipetting device based on a cell slide staining machine proposed in this utility model;

[0026] Figure 4 This is a partial structural diagram of the integrated pipetting device based on a cell slide staining machine proposed in this utility model;

[0027] Figure 5 This is a partial structural exploded view of the integrated pipetting device based on a cell slide staining machine proposed in this utility model;

[0028] Figure 6 This is a partial structural exploded view of the integrated pipetting device based on a cell slide staining machine proposed in this utility model.

[0029] Legend:

[0030] 1. Frame; 2. Uniform mixing mechanism; 201. Fixing block; 202. Motor II; 203. Vibrating wheel; 204. Pipette; 205. Vibrating plate; 206. Groove II; 207. Conducting plate; 208. Shock-absorbing ring; 209. Fixing box; 3. Groove I; 4. Ultraviolet tube; 5. Heating box; 6. Dispersion plate; 7. Temperature sensor; 8. Control line; 9. Cooling box; 10. Motor I; 11. Fan; 12. Heating wire; 13. Cooling element; 14. Air outlet; 15. Support column; 16. Transmission ring I; 17. Conveyor belt; 18. Supporting block; 19. Transmission ring II; 20. Threaded rod; 21. Threaded sleeve rod; 22. Movable column; 23. Fixing plate; 24. Motor; 25. Base plate; 26. Sliding bar; 27. Slide groove; 28. Dyeing machine. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Please see the appendix Figure 3 - Appendix Figure 6This utility model provides an embodiment of an integrated pipetting device based on a cell slide staining machine, comprising a frame 1. Grooves 3 are formed on both sides of the inner wall of the frame 1. Ultraviolet tubes 4 are fixedly connected to the inner walls of both grooves 3 to sterilize the inside of the device. A thermometer 7 is fixedly connected to the left side of the inner wall of the frame 1. A control line 8 is fixedly connected to the outer wall of the thermometer 7 to detect and control the temperature. A heating box 5 is fixedly connected to the bottom rear side of the control line 8. A motor 10 is fixedly connected to the bottom inner wall of the heating box 5 for fixation. The output end of the motor 10... A fan 11 is fixedly connected. A heating wire 12 is fixedly connected to the middle of the inner wall of the heating box 5 for heating. A dispersion plate 6 is fixedly connected to the front side of the inner wall of the heating box 5. A cooling box 9 is fixedly connected to the top of the outer wall of the control line 8 for fixed connection. A cooling plate 13 is fixedly connected to the bottom of the inner wall of the cooling box 9. An air outlet 14 is connected to the bottom of the outer wall of the cooling plate 13 for cooling. A dispersion plate 6 is fixedly connected to the front side of the inner wall of the cooling box 9. A uniform mixing mechanism 2 is provided in the middle of the inner side of the frame 1 for uniform mixing of the solution.

[0033] Specifically, the temperature inside the equipment is first detected by the thermometer 7. If the temperature is too low, the heating wire 12 fixed in the middle of the inner wall of the heating box 5 generates heat. Then, the motor 10 fixed in front of the inner wall of the heating box 5 is started. The output end of the motor 10 is fixedly connected to the fan 11, which blows out the hot air. If the temperature is too high, the cooling plate 13 fixed in the inner wall of the cooling box 9 generates cold air, which is blown out through the air outlet 14. In this way, the internal temperature of the equipment is regulated. At the same time, the ultraviolet tube 4 can disinfect the inside of the equipment.

[0034] Please see the appendix Figure 1 - Appendix Figure 3 The uniform mixing mechanism 2 includes a fixed block 201. The left side of the outer wall of the fixed block 201 is slidably connected to the right side of the inner wall of the frame 1. A fixed box 209 is fixedly connected to the rear side of the outer wall of the fixed block 201 for fixing. A motor 202 is fixedly connected to the top of the inner wall of the fixed box 209. A vibrating wheel 203 is fixedly connected to the output end of the motor 202. A vibrating plate 205 is fixedly connected to the rear bottom of the fixed block 201. Vibration is achieved by the vibrating wheel 203 striking the vibrating plate 205. A groove 206 is provided on the rear side of the outer wall of the vibrating plate 205. A transmission plate 207 is fixedly connected to the rear side of the outer wall of the vibrating plate 205 for transmitting vibration. A pipette 204 is fixedly connected to the bottom of the outer wall of the fixed block 201. A damping ring 208 is fixedly connected to the outer wall of the pipette 204 for shock absorption.

[0035] Specifically, a vibrating wheel 203 is fixedly connected to the output end of motor 202. Motor 202 drives the vibrating wheel 203. The outer wall of the vibrating wheel 203 contacts the vibrating plate 205. Vibration is generated by the vibrating wheel 203 striking the vibrating plate 205. A conduction plate 207 is fixedly connected to the outer wall of the vibrating plate 205. Shock-absorbing rings 208 are fixedly connected to both sides of the conduction plate 207 to play a shock-absorbing role. A pipette 204 is fixedly connected to the inner wall of the shock-absorbing ring 208. In this way, uniform mixing of the solution is achieved.

[0036] Please see the appendix Figure 1 - Appendix Figure 3 A support column 15 is fixedly connected to the left side of the outer wall of the frame 1. A motor 24 is fixedly connected to the top of the support column 15 for fixed connection. A transmission ring 29 is fixedly connected to the output end of the motor 24. A conveyor belt 17 is connected to the middle of the outer wall of the transmission ring 29 for transmission. A transmission ring 16 is connected to the left side of the conveyor belt 17 for transmission. A threaded rod 20 is fixedly connected to the bottom of the transmission ring 16 for lifting. A threaded sleeve rod 21 is threadedly connected to the outer wall of the threaded rod 20.

[0037] Specifically, the support column 15 is fixedly connected to the transmission ring 16, and the transmission ring 19 is connected to the transmission ring 16 by the conveyor belt 17 to play a transmission role. The bottom of the transmission ring 19 is fixedly connected to the threaded rod 20, and the threaded rod 20 is threadedly connected to the threaded sleeve rod 21 to play a lifting and lowering role.

[0038] Please see the appendix Figure 1 - Appendix Figure 3 The outer wall of the threaded sleeve 21 is threadedly connected to a movable column 22. A fixed plate 23 is fixedly connected to the middle of the outer wall of the movable column 22 for fixing. A support block 18 is fixedly connected to the top of the frame 1. A base plate 25 is fixedly connected to the bottom of the frame 1 for fixing. A sliding groove 27 is provided on the top of the outer wall of the base plate 25. A sliding strip 26 is fixedly connected to the middle of the inner wall of the sliding groove 27 for the dyeing machine 28 to move. The dyeing machine 28 is slidably connected to the middle of the outer wall of the base plate 25.

[0039] Specifically, the base plate 25 is fixed by a fixing plate 23, and a groove 27 is provided on the outer wall of the base plate 25 to allow the sliding bar 26 to move. The sliding bar 26 is slidably connected to the dyeing machine 28, and the sliding bar 26 drives the dyeing machine 28 to move.

[0040] Working principle: A thermometer 7 is fixedly connected inside the equipment to sense and control the internal temperature. When the internal temperature is too low, the heating wire 12 heats up and dissipates heat. Then, the motor 10 is started, and the output end of the motor 10 is fixedly connected to the fan 11, which drives the fan 11 to rotate and blow out the hot air. At the same time, a dispersion plate 6 is fixedly connected at the box opening to better disperse the hot air and prevent local overheating. When the temperature is too high, the thermometer 7 controls the cooling plate 13 to generate cold air, which is then delivered through the air outlet 14. Similarly, a dispersion plate 6 is fixedly connected at the box opening to prevent local overcooling. In this way, the internal temperature of the equipment is regulated. At the same time, an ultraviolet tube 4 is fixedly connected inside to sterilize the inside of the equipment.

[0041] When uniform mixing of the solution is required, the motor 202, which is fixedly connected to the right side of the outer wall of the fixed block 201, is activated. The output end of the motor 202 is fixedly connected to the vibrating wheel 203. The bottom right side of the fixed block 201 is fixedly connected to the vibrating plate 205. The outer right side of the vibrating plate 205 has a groove 206. The motor 202 drives the vibrating wheel 203 to strike the vibrating plate 205 and generate vibration. At the same time, the outer right side of the vibrating plate 205 is fixedly connected to the conduction plate 207. Multiple conduction plates 207 are fixedly connected to both sides of the shock-absorbing rings 208, which play a shock-absorbing role. The inner walls of multiple shock-absorbing rings 208 are fixedly connected to the pipettes 204, thus achieving uniform dispersion of the solution.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An integrated pipetting device based on a cell slide staining machine, comprising a frame (1), characterized in that: The inner wall of the frame (1) has grooves (3) on both sides. Ultraviolet tubes (4) are fixedly connected to the inner walls of both grooves (3). A thermometer (7) is fixedly connected to the left side of the inner wall of the frame (1). A control line (8) is fixedly connected to the outer wall of the thermometer (7). A heating box (5) is fixedly connected to the bottom rear side of the control line (8). A motor (10) is fixedly connected to the bottom inner wall of the heating box (5). A fan (11) is fixedly connected to the output end of the motor (10). The middle part of the inner wall of the heating box (5) A heating wire (12) is fixedly connected to the heating box (5). A dispersion plate (6) is fixedly connected to the front side of the inner wall of the heating box (5). A cooling box (9) is fixedly connected to the top of the outer wall of the control line (8). A cooling plate (13) is fixedly connected to the bottom of the inner wall of the cooling box (9). An air outlet (14) is connected to the bottom of the outer wall of the cooling plate (13). A dispersion plate (6) is fixedly connected to the front side of the inner wall of the cooling box (9). A uniform mixing mechanism (2) is provided in the middle of the inner side of the frame (1). The uniform mixing mechanism (2) is used for uniform mixing of the solution.

2. The integrated pipetting device based on a cell slide staining machine according to claim 1, characterized in that: The uniform mixing mechanism (2) includes a fixed block (201). The left side of the outer wall of the fixed block (201) is slidably connected to the right side of the inner wall of the frame (1). A fixed box (209) is fixedly connected to the rear side of the outer wall of the fixed block (201). A motor (202) is fixedly connected to the top of the inner wall of the fixed box (209). A vibrating wheel (203) is fixedly connected to the output end of the motor (202). A vibrating plate (205) is fixedly connected to the rear bottom side of the fixed block (201). A groove (206) is provided on the rear side of the outer wall of the vibrating plate (205). A conduction plate (207) is fixedly connected to the rear side of the outer wall of the vibrating plate (205). A pipette (204) is fixedly connected to the bottom of the outer wall of the fixed block (201). A damping ring (208) is fixedly connected to the outer wall of the pipette (204).

3. The integrated pipetting device based on a cell slide staining machine according to claim 1, characterized in that: A support column (15) is fixedly connected to the left side of the outer wall of the frame (1), and an electric motor (24) is fixedly connected to the top of the support column (15).

4. The integrated pipetting device based on a cell slide staining machine according to claim 3, characterized in that: The output end of the motor (24) is fixedly connected to a transmission ring two (19), and a transmission belt (17) is connected to the middle of the outer wall of the transmission ring two (19). A transmission ring one (16) is connected to the left side of the transmission belt (17).

5. The integrated pipetting device based on a cell slide staining machine according to claim 4, characterized in that: The bottom of the transmission ring (16) is fixedly connected to a threaded rod (20), and the outer wall of the threaded rod (20) is threadedly connected to a threaded sleeve rod (21).

6. The integrated pipetting device based on a cell slide staining machine according to claim 5, characterized in that: The outer wall of the threaded sleeve (21) is threadedly connected to a movable column (22), and a fixed plate (23) is fixedly connected to the middle of the outer wall of the movable column (22).

7. The integrated pipetting device based on a cell slide staining machine according to claim 1, characterized in that: A support block (18) is fixedly connected to the top of the frame (1), and a base plate (25) is fixedly connected to the bottom of the frame (1).

8. The integrated pipetting device based on a cell slide staining machine according to claim 7, characterized in that: The top of the outer wall of the base plate (25) is provided with a sliding groove (27), a sliding strip (26) is fixedly connected to the middle of the inner wall of the sliding groove (27), and a dyeing machine (28) is slidably connected to the middle of the outer wall of the base plate (25).

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