Magnetic separation device

Abstract

The utility model discloses a magnetic separation device which comprises a bottom plate, a lifting driving mechanism, a rotary driving mechanism and an online uniform mixing mechanism are arranged on the bottom plate, the lifting driving mechanism is connected with a head washing assembly, the head washing assembly comprises an arc-shaped plate, five sets of elution needles and a set of liquid suction needles are uniformly arranged on the arc-shaped plate at intervals, and the arc-shaped plate is connected with the rotary driving mechanism. One end, close to the liquid suction needle, of the arc-shaped plate is provided with a set of substrate needles through a connecting plate, and the arc-shaped plate is further provided with an anti-collision mechanism; the rotary driving mechanism is connected with the driving disc, the driving disc and the arc-shaped plate are concentrically arranged, reaction cup clamping and placing holes which are uniformly distributed in the circumferential direction are formed in the driving disc, a magnetic field generator and a water receiving tank are arranged below the reaction cup clamping and placing holes, and the online uniform mixing mechanism corresponds to one reaction cup clamping and placing hole. The magnetic separation device provided by the utility model is simple in structure, small in size, low in cost and low in failure rate, and adopts five-order magnetic separation, so that immune complexes are fully cleaned.

Description

Technical Field

[0001] This utility model relates to the field of trace analysis and detection equipment technology, and in particular to a magnetic separation device. Background Technology

[0002] Chemiluminescent immunoassay (CIA) is a microanalytical detection technique that combines the high sensitivity of chemical reactions with the high specificity of immunoassay. It is one of the most advantageous quantitative immunoassay methods developed after enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and fluorescence immunoassay. Magnetic separation is a crucial step in CIA. The magnetic separation process involves cleaning the magnetic beads within the reaction vessel. Dispersing the magnetic beads facilitates cleaning within the reaction vessel. Common methods for dispersing magnetic beads include mechanical dispersion and magnet arrangement. Mechanical dispersion is complex, costly, and has a high failure rate. Magnet arrangement can achieve both dispersion and aggregation of magnetic beads, but in some existing magnet arrangement structures, the beads remain in a dry state for a long time in the reaction vessel, leading to agglomeration and poor cleaning, resulting in more impurities within the immune complex and affecting experimental results. In some cases, the number of cleaning passes is insufficient, and impurities within the immune complex are not completely removed, also affecting experimental results. In addition to the above, existing magnetic separation devices also have the following problems in use: the magnetic separation device has a complex structure, large size, and occupies a large area; when placing the reaction vessel, liquid splashing is easy to occur, which is not conducive to cleaning and can easily lead to inconsistent experimental results; long-term heating of the substrate causes volatilization and partial denaturation, which affects the results; the substrate mixing mechanism has a relatively complex structure, high cost, and high failure rate; there is no anti-collision trigger alarm function when the washing head descends, which can lead to damage to the liquid injection needle or failure to predict and alarm for erroneous results; crystals are prone to form on the drive plate, requiring frequent cleaning; the liquid flow in the water tank is not smooth, and when crystals accumulate, blockage occurs. Summary of the Invention

[0003] To solve the above problems, this utility model provides a magnetic separation device, which can be specifically implemented using the following technical solution:

[0004] The magnetic separation device of this utility model includes a base plate, on which a lifting drive mechanism, a rotating drive mechanism, and an online mixing mechanism are provided. The lifting drive mechanism is connected to a shampoo assembly, which includes an arc-shaped plate. Five sets of elution needles and one set of suction needles are evenly spaced on the arc-shaped plate, and a set of substrate needles is provided at one end of the arc-shaped plate near the suction needles via a connecting plate. An anti-collision mechanism is also provided on the arc-shaped plate. The rotating drive mechanism is connected to a drive disk, which is concentrically arranged with the arc-shaped plate. The drive disk has reaction cup placement holes evenly distributed circumferentially. A magnetic field generator and a water receiving tank are provided below each reaction cup placement hole. The online mixing mechanism is correspondingly arranged with one of the reaction cup placement holes.

[0005] The lifting drive mechanism includes a lead screw motor mounted on the base plate. The lifting nut of the lead screw motor is fixedly connected to the arc plate. Vertical guide rods are also provided on both sides of the lead screw of the lead screw motor. The vertical guide rods are connected to the arc plate through linear bearings.

[0006] The elution needles are used for liquid aspiration and injection, including sleeve-type elution needles and parallel elution needles; both the elution needles and the aspiration needles are connected to the arc-shaped plate by equal-height screws fitted with compression springs.

[0007] The anti-collision mechanism includes anti-collision posts that pass through the arc plate, a photoelectric sensor that is correspondingly arranged above the anti-collision post, and a buffer spring that is arranged between the photoelectric sensor and the anti-collision post.

[0008] The rotary drive mechanism includes a stepper motor disposed below the base plate. The output shaft of the stepper motor is connected to the central shaft of the drive disk through a belt drive assembly. The drive disk and the magnetic field generator are spaced apart. Multiple lugs are evenly spaced around the periphery of the drive disk, and each lug corresponds to a reaction cup mounting hole.

[0009] The water receiving trough is an arc-shaped trough corresponding to the position of the arc-shaped plate. It is set on the bottom plate, with a drainage hole at each end, and the bottom surface is provided with a slope that descends from the middle to both ends.

[0010] The magnetic field generator is mounted on the water receiving tank and includes an inner magnetic support and an outer magnetic support respectively mounted on the inner and outer sides of the reaction cup. Magnetic components are mounted on both the inner and outer magnetic supports.

[0011] The online mixing mechanism is mounted on the base plate via fixed columns and includes a driver and a mixer. The output shaft of the driver is fixedly connected to a rotating shaft. A radially extending guide column is provided on the rotating shaft. The mixer is sleeved on the rotating shaft. A spiral groove for passing through the guide column is provided on the side wall of the mixer. A support is provided on the top of the mixer. A support groove for eccentrically placing the reaction cup is opened on the top of the support. A counterweight is provided at the bottom of the mixer.

[0012] The mixer has an exhaust hole on its side wall, and the support has a drain hole on its side wall that communicates with the support groove.

[0013] The base plate is also provided with a splash-proof mechanism, which includes a mounting base fixedly mounted on the base plate. The mounting base has a cavity for receiving the reaction cup at the top center. Buffer cotton sheets that connect with the side walls of the reaction cup are provided on both sides of the top of the mounting base. The buffer cotton sheets are fixed by L-shaped pressure plates, and the L-shaped pressure plates are fixed to the side walls of the mounting base by bolts.

[0014] The magnetic separation device provided by this invention has a simple structure, small size, low cost, and low failure rate. Because the elution needles are integrated into a sleeve-type and parallel-type elution needle, liquid aspiration is followed by liquid injection, reducing the dry time of the immune complexes containing magnetic beads. This allows the liquid medium to be isolated between the immune complexes, preventing strong magnetic acceleration that could cause sticking and prevent cleaning, or the immune complexes from agglomerating and becoming difficult to mix, leading to inaccurate experimental results. Fifth-order magnetic separation is used, ensuring thorough cleaning of the immune complexes with low background. A flexible buffer cotton sheet is placed below the inlet; during the insertion and pressing of the reaction vessel, the flexible material is the first to come into contact, preventing visible splashing and its impact on experimental results. The reaction vessel is secured on the drive plate. The holes are spaced apart, with a hollow space between the two insertion holes. This hollow space is reserved for injecting substrate into the water tank before the actual substrate is added, thus solving the problem of variations in the first hole results. The substrate mixing uses a single motor for automatic upward rotation and eccentric mixing, and automatically descends to make room when stopped. This provides good mixing effect and occupies little space. It has a head-washing descent anti-collision function. If the reaction vessel position or turntable rotation is abnormal, the head-washing descent anti-collision function can prevent damage to the injection needle and provide an alarm function for potential problems with the results. The drive plate adopts a hollow structure, so that more droplets attached to the injection needle fall into the water tank, reducing the need for manual cleaning due to crystal formation. The water tank is equipped with a slope and double-sided outlets to prevent crystal blockage and ensure smooth water discharge. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model.

[0016] Figure 2 yes Figure 1 A schematic diagram of the shampoo head assembly.

[0017] Figure 3 yes Figure 1 A schematic diagram of the structure of the components after removing the shampooing unit.

[0018] Figure 4 yes Figure 3 Cross-sectional view.

[0019] Figure 5 yes Figure 3 A schematic diagram showing the exploded structure of the intermediate water tank and magnetic field generator.

[0020] Figure 6 yes Figure 4 Enlarged view of the medium magnetic field generator section.

[0021] Figure 7 yes Figure 4 Enlarged view of the splash-proof mechanism.

[0022] Figure 8 yes Figure 1A schematic diagram of the online mixing mechanism.

[0023] Figure 9 make Figure 8 Cross-sectional view.

[0024] Figure 10 yes Figure 8 A three-dimensional structural diagram of the mixer and the support.

[0025] Figure 11 This is a speed-time relationship diagram of the driver in the embodiment. Detailed Implementation

[0026] The embodiments of this utility model will be described in detail below with reference to the accompanying drawings. These embodiments are implemented based on the technical solution of this utility model and provide detailed implementation methods and specific working processes. However, the protection scope of this utility model is not limited to the following embodiments.

[0027] like Figures 1-11 As shown, the magnetic separation device of this utility model comprises a base plate 1, a drive plate 2, a shampooing assembly, a lifting drive mechanism, a rotating drive mechanism, an online mixing mechanism, a splash-proof mechanism, an anti-collision mechanism, a magnetic field generator, and a water receiving tank. The lifting drive mechanism, rotating drive mechanism, online mixing mechanism, splash-proof mechanism, and water receiving tank are connected to the base plate 1. The drive plate 2 is connected to the rotating drive mechanism. The shampooing assembly is positioned above the drive plate 2 and connected to the lifting drive mechanism.

[0028] Specifically, the shampoo assembly includes an arc-shaped plate 31, on which five sets of rinsing needles 32 and one set of suction needles 33 are arranged at even intervals. A set of substrate needles 34 is arranged at one end of the arc-shaped plate 31 near the suction needles 33 via a connecting plate. The central axes of the rinsing needles 32, suction needles 33, and substrate needles 34 are arranged in a circular pattern.

[0029] The elution needle 32 is used for liquid aspiration and injection, and includes one sleeve-type elution needle and four parallel elution needles (see...). Figure 2 The sleeve-type elution needle has a cleaning and aspiration needle function to prevent BF from being carried. The aspiration needle 33 is used for aspiration to achieve fifth-order magnetic separation; the substrate needle 34 is used for substrate addition. Both the elution needle 32 and the aspiration needle 33 are connected to the arc-shaped plate 31 by equal-height screws 36 with compression springs 35 attached. When the needle tip touches the bottom, it can form a certain buffering effect to protect the needle tip from damage.

[0030] Furthermore, an anti-collision mechanism is provided on the arc-shaped plate 31, which includes an anti-collision post 41 passing through the arc-shaped plate 31. A photoelectric sensor 42 is correspondingly arranged above the anti-collision post 41. The photoelectric sensor 42 is fixedly connected to the arc-shaped plate 31, and a buffer spring 43 is provided between the photoelectric sensor 42 and the anti-collision post 41. When the anti-collision post 41 is hit, it will push up and trigger the photoelectric sensor 42, thereby protecting the elution needle 32 and the aspiration needle 33.

[0031] To enable operations such as adding liquid, the aforementioned shampoo assembly is connected to a lifting drive mechanism. In this embodiment, the lifting drive mechanism includes a lead screw motor 51 mounted on the base plate 1. The lifting nut of the lead screw motor 51 is fixedly connected to the arc-shaped plate 31. Vertical guide rods 52 are also provided on both sides of the lead screw of the lead screw motor 51, and the vertical guide rods 52 are connected to the arc-shaped plate 31 through linear bearings 53.

[0032] Below the shampoo assembly is a drive disk 2 connected to a rotary drive mechanism. In this embodiment, the rotary drive mechanism includes a stepper motor 61 disposed below the base plate 1, and the output shaft of the stepper motor 61 is connected to the central shaft of the drive disk 2 via a belt drive assembly 62.

[0033] The drive disk 2 has a hollow structure and is concentrically positioned with the arc-shaped plate 31 (i.e., the center of the drive disk 2 coincides with the central axis of the elution needle 32, the aspiration needle 33, and the substrate needle 34). Multiple lugs 21 are evenly spaced around the periphery of the drive disk 2, each lug 21 corresponding to a reaction cup insertion hole 22. Because the spaces between adjacent reaction cup insertion holes 22 are hollow, they can serve as reserved positions for empty substrate injection. Before the formal substrate injection, the substrate can be injected into the water collection tank 7 in these two empty positions, solving the problem of variations in the first well result. Secondly, more droplets adhering to the injection needles can fall into the water collection tank, reducing the need for manual cleaning due to crystal formation on the drive disk 2.

[0034] Below the drive plate 2 are a magnetic field generator 8 and a water receiving tank 7 arranged sequentially, with the drive plate 2 and the magnetic field generator 8 spaced apart. The water receiving tank 7 is an arc-shaped groove corresponding to the position of the arc plate 31 (i.e., the tips of the elution needle 32, the suction needle 33, and the substrate needle 34 correspond to the arc-shaped center line of the water receiving tank 7). It is fixedly installed on the base plate 1, with a drain hole at each end, and the bottom surface has a slope that descends from the middle to both ends. This structure ensures smooth water discharge from the water receiving tank 7 and avoids crystallization blockage. The magnetic field generator 8 is installed on the water receiving tank and includes an inner magnetic support 81 and an outer magnetic support 82 (i.e., corresponding to the inner and outer sides of the reaction cup insertion hole 22), respectively located on the inner and outer sides of the reaction cup M. Magnetic components 83 are installed on both the inner and outer magnetic supports 81 and 82.

[0035] In this embodiment, when installing the magnetic components 83, the distance between the top of the magnetic component 83 and the bottom of the reaction cup M should be 6.85 mm. Specifically, the N-grade of the first three magnetic components 83 in the inner magnetic component support 81 faces the reaction cup M, the S-grade of the fourth and fifth magnetic components 83 faces the reaction cup M, and the S-grade of the eighth, ninth, twelfth, and thirteenth magnetic components 83 faces the reaction cup M; the S-grade of the first three magnetic components 83 in the outer magnetic component support 82 faces the reaction cup M, the S-grade of the sixth and seventh magnetic components 83 faces the reaction cup M, and the S-grade of the tenth and eleventh magnetic components 83 faces the reaction cup M. The arrangement of the magnetic components 83 in this manner achieves better alignment of the magnetic beads within the reaction cup M, allowing impurities within the immune complex to be released in the liquid medium, resulting in more thorough cleaning of the immune complex.

[0036] When the drive disk 2 rotates under the action of the rotary drive mechanism, the reaction cup M on it moves between the outer magnetic component support 82 and the inner magnetic component support 81, and the magnetic component 83 provides a stable magnetic field for the reaction cup M.

[0037] To prevent splashing when the gripper places the reaction cup M, a splash-proof mechanism is provided on the base plate 1. The splash-proof mechanism includes a mounting base 91 fixedly mounted on the base plate 1. A reaction cup receiving groove 92 is provided at the center of the top of the mounting base 91. Buffer cotton sheets 93 are provided on both sides of the top of the mounting base 91, which are in contact with the side walls of the reaction cup M. The buffer cotton sheets 93 are fixed by L-shaped pressure plates 94, and the L-shaped pressure plates 94 are fixed to the side walls of the mounting base 91 by bolts 95.

[0038] When the reaction cup M is placed onto the drive plate 2 by the gripper device, the reaction cup mounting hole 22 is aligned with the reaction cup receiving groove 92. When the reaction cup M passes through the buffer cotton sheet 93, the buffer cotton sheet 93 provides some resistance to the reaction cup M, preventing the reaction cup M from free-falling and avoiding collision with the drive plate 2 due to rapid falling. This also prevents the liquid inside the reaction cup M from splashing out, thus playing a role in preventing liquid splashing.

[0039] To ensure more uniform mixing of the liquid within the reaction vessel M, an online mixing mechanism is also installed on the base plate 1. This online mixing mechanism includes a driver and a mixer.

[0040] In this embodiment, the driver is a unidirectional motor 1001. Of course, a reversible motor can also be used as the driver. The output shaft of the unidirectional motor 1001 is fixedly connected to the rotating shaft 1002 by a set screw 1003, and the two rotate synchronously. A radially extending guide post 1004 is also fixed on the rotating shaft 1002, which is used to connect the mixer 1005.

[0041] The mixer 1005 is made of wear-resistant Iglidur®J engineering plastic and is fitted onto the rotating shaft 1002. A spiral channel 1006 for the guide post 1004 to pass through is formed on its side wall. When the unidirectional motor 1001 rotates and there is a speed difference between the rotating shaft 1002 and the mixer 1005, the mixer 1005 will rise or fall along the rotating shaft 1002, that is, the guide post 1004 will slide upward or downward along the spiral channel 1006. Because the clearance between the rotating shaft 1002 and the mixer 1005 is small, an exhaust hole 1007 is formed on the side wall of the mixer 1005 to ensure smooth relative movement between the rotating shaft 1002 and the mixer 1005. Secondly, a continuous convex rib 1008 is provided on the spiral channel 1006 to connect with the guide post 1004. The width of the contact surface between the convex rib 1008 and the guide post 1004 is smaller than the wall thickness of the mixer 1005. This increases the contact area between the guide post 1004 and the spiral channel 1006, reduces friction, and ensures smooth relative movement between the rotating shaft 1002 and the mixer 1005. Thirdly, the bottom end of the spiral channel 1006 is horizontally positioned (i.e., a horizontal section is provided), and a boss 1009 is provided at the inlet of this horizontal section to reduce the channel diameter. This ensures that the mixer 1005 will not fall when it reaches its highest position. Finally, a counterweight 1010 is provided at the bottom of the mixer 1005. This counterweight 1010 is a counterweight ring coaxially arranged with the mixer 1005, used to lower the center of gravity of the mixer 1005 and ensure the operational stability of the mixer 1005.

[0042] The mixer 1005 is equipped with a support 1011 at its top. The support 1011 has an open-top support groove 1012 at its top, with the central axis of the support groove 1012 parallel to the central axis of the rotation shaft 1002; that is, the support groove 1012 is an eccentric groove. When the mixer 1005 rotates the support 1011, the sample to be tested within the eccentric support groove 1012 can be well mixed. A drain hole 1013, communicating with the support groove 1012, is provided on the side wall of the support 1011 to prevent accidental sample spillage and subsequent accumulation within the support groove 1012.

[0043] In addition, a fixing post 1014 extending upward is installed at each of the four corners of the top of the housing of the unidirectional motor 1001, which is used to fix the device to the online analysis equipment.

[0044] During the lifting and mixing process, the rotating shaft 1002 continuously accelerates, driving the mixer 1005 to rotate. The operating speed of the mixer 1005 is always less than the operating speed of the rotating shaft 1002, thus ensuring the stable operation of the mixer 1005. The unidirectional motor 1001 has the following three speed control stages:

[0045] In the first step, the output shaft of the unidirectional motor 1001 enters the first uniform acceleration stage, which is used to make the mixer 1005 rise. During this process, the guide column 1004 moves from the top starting end of the spiral channel 1006 to the bottom ending end of the spiral channel 1006.

[0046] In the second step, the output shaft of the unidirectional motor 1001 enters the second uniform acceleration stage, which is used to keep the mixer 1005 rotating at the highest position. During this process, the speed of the output shaft of the unidirectional motor 1001 is always greater than its speed in the first uniform acceleration stage.

[0047] In the third step, the output shaft of the unidirectional motor 1001 first rotates at a constant speed and then enters a uniform deceleration stage until the speed is zero. During this process, the mixer 1005 descends and the guide column 1004 moves from the bottom end of the spiral channel 1006 to the top start end of the spiral channel 1006.

[0048] The working process of the above-mentioned online mixing mechanism is explained in detail below:

[0049] First uniform acceleration phase (0-0.2 seconds): The rotating shaft 1002 accelerates clockwise under the action of the unidirectional motor 1001. The mixer 1005 rotates relative to the rotating shaft 1002 under the action of static inertia. During the relative rotation of the mixer 1005 and the rotating shaft 1002, the guide post 1004 on the rotating shaft 1002 provides a force along the direction of rotation. This force acts on the inclined surface of the spiral channel 1006 of the mixer 1005 and is decomposed into a force along the direction of rotation and a force perpendicular to the direction of rotation and upward. The force along the direction of rotation causes the mixer 1005 to accelerate with the rotating shaft 1002. The force perpendicular to the direction of rotation and upward causes the mixer 1005 to rise against its own weight and the weight of the counterweight 1010.

[0050] At this time, if the unidirectional motor 1001 is still in the first uniform acceleration state, due to the inertia of the mixer 1005, the mixer 1005 will collide with the guide post 1004 the instant it rises to the highest position. After the collision, the guide post 1004 will detach from the horizontal section at the bottom end of the spiral channel 1006 and eventually fall, resulting in the failure to rise.

[0051] Therefore, when the guide post 1004 reaches the bottom end of the spiral channel 1006, the unidirectional motor 1001 will continue to accelerate into the second uniform acceleration stage.

[0052] The second uniform acceleration phase (0.2-3.7 seconds): the output shaft of the unidirectional motor 1001 follows... Figure 4The second segment of the six-point curve shown accelerates to overcome the collision between the mixer 1005 and the guide post 1004 caused by the inertia of the mixer 1005. The acceleration force of the second segment controlled by the six-point curve is greater than the force of the collision between the mixer 1005 and the guide post 1004, so that the rotating shaft 1002 continuously accelerates and rotates, driving the mixer 1005 to rotate. That is, the running speed of the mixer 1005 is always less than the running speed of the rotating shaft 1002, thereby achieving stable operation of the mixer 1005.

[0053] The aforementioned unidirectional motor 1001 rotates by receiving pulses. After receiving a pulse sequence, the unidirectional motor 1001 starts rotating. However, the pulse frequency received by the unidirectional motor 1001 fluctuates, causing its operation to be non-uniform. During this non-uniform operation, when the speed of the rotating shaft 1002 is less than the speed of the mixer 1005, the mixer 1005 will fall off, resulting in mixing failure. In this invention, the speed of the unidirectional motor 1001 is controlled according to the second segment of a six-point curve. This overcomes the speed fluctuations caused by the fluctuating pulse frequency, ensuring that the rotating shaft 1002 continuously accelerates, driving the mixer 1005 to rotate. Even if the speed of the mixer 1005 is always less than the speed of the rotating shaft 1002, this ensures stable operation of the mixer 1005.

[0054] When the mixer 1005 is running stably, the guide post 1004 is in the horizontal groove at the bottom of the mixer 1005. At this time, the force exerted by the guide post 1004 on the mixer 1005 is a force along the rotation direction and a force perpendicular to the rotation direction and downward. Since the boss 1009 is provided at the inlet of the horizontal section of the spiral channel 1006, the resistance to the guide post 1004 leaving the horizontal section of the spiral channel 1006 is increased. Therefore, it is further ensured that the mixer 1005 will not fall when rotating at the top.

[0055] Uniform speed buffering phase (3.7-4 seconds): When mixing is complete, the output shaft of the unidirectional motor 1001 follows... Figure 4 The third segment of the six-point curve shown maintains a constant speed to buffer the subsequent deceleration motion and protect the unidirectional motor 1001 from damage.

[0056] Uniform deceleration phase (4-4.2 seconds): During this phase, the output shaft of the unidirectional motor 1001 decelerates according to... Figure 4 The fourth segment of the six-point curve shown enters the uniform deceleration stage until the speed reaches zero.

[0057] During the uniform speed buffering stage and uniform deceleration stage, the mixer 1005 loses the driving force of the rotating shaft 1002. When the rotating shaft 1002 stops rotating, the mixer 1005 continues to rotate clockwise under the action of inertia. Since the rotating shaft 1002 and the guide column 1004 are stationary, the guide column 1004 will disengage from the horizontal groove of the mixer 1005. Under the action of gravity of the mixer 1005 and the counterweight 1010, the mixer 1005 descends to the lowest position and finally reaches a stationary state.

[0058] The aforementioned online mixing mechanism can be well applied to online analytical equipment. It features a simple structure, compact size, ease of operation, stable operation, and low failure rate. It eliminates the need for additional locking devices or magnetic chucks to prevent the mixer from falling; simply controlling the drive's speed ensures stable rising and high-position rotation of the mixer, even during the continuous acceleration process. Once mixing is complete, the drive simply decelerates. This online mixing mechanism improves the overall space utilization of the equipment and reduces its footprint.

[0059] It should be noted that in the description of this utility model, terms such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", and "outer" indicating orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

Claims

1. A magnetic separation device, characterized in that: The device includes a base plate, on which a lifting drive mechanism, a rotating drive mechanism, and an online mixing mechanism are provided. The lifting drive mechanism is connected to a shampoo assembly, which includes an arc-shaped plate. Five sets of elution needles and one set of suction needles are evenly spaced on the arc-shaped plate, and a set of substrate needles is provided at one end of the arc-shaped plate near the suction needles via a connecting plate. An anti-collision mechanism is also provided on the arc-shaped plate. The rotating drive mechanism is connected to a drive disk, which is concentrically arranged with the arc-shaped plate. The drive disk has reaction cup placement holes evenly distributed circumferentially. A magnetic field generator and a water receiving tank are provided below each reaction cup placement hole. The online mixing mechanism is correspondingly arranged with one of the reaction cup placement holes.

2. The magnetic separation device according to claim 1, characterized in that: The lifting drive mechanism includes a lead screw motor mounted on the base plate. The lifting nut of the lead screw motor is fixedly connected to the arc plate. Vertical guide rods are also provided on both sides of the lead screw of the lead screw motor. The vertical guide rods are connected to the arc plate through linear bearings.

3. The magnetic separation device according to claim 1, characterized in that: The elution needles are used for liquid aspiration and injection, including sleeve-type elution needles and parallel elution needles; both the elution needles and the aspiration needles are connected to the arc-shaped plate by equal-height screws fitted with compression springs.

4. The magnetic separation device according to claim 1, characterized in that: The anti-collision mechanism includes anti-collision posts that pass through the arc plate, a photoelectric sensor that is correspondingly arranged above the anti-collision post, and a buffer spring that is arranged between the photoelectric sensor and the anti-collision post.

5. The magnetic separation device according to claim 1, characterized in that: The rotary drive mechanism includes a stepper motor disposed below the base plate. The output shaft of the stepper motor is connected to the central shaft of the drive disk through a belt drive assembly. The drive disk and the magnetic field generator are spaced apart. Multiple lugs are evenly spaced around the periphery of the drive disk, and each lug corresponds to a reaction cup mounting hole.

6. The magnetic separation device according to claim 1, characterized in that: The water receiving trough is an arc-shaped trough corresponding to the position of the arc-shaped plate. It is set on the bottom plate, with a drainage hole at each end, and the bottom surface is provided with a slope that descends from the middle to both ends.

7. The magnetic separation device according to claim 1, characterized in that: The magnetic field generator is mounted on the water receiving tank and includes an inner magnetic support and an outer magnetic support respectively mounted on the inner and outer sides of the reaction cup. Magnetic components are mounted on both the inner and outer magnetic supports.

8. The magnetic separation device according to claim 1, characterized in that: The online mixing mechanism is mounted on the base plate via fixed columns and includes a driver and a mixer. The output shaft of the driver is fixedly connected to a rotating shaft. A radially extending guide column is provided on the rotating shaft. The mixer is sleeved on the rotating shaft. A spiral groove for passing through the guide column is provided on the side wall of the mixer. A support is provided on the top of the mixer. A support groove for eccentrically placing the reaction cup is opened on the top of the support. A counterweight is provided at the bottom of the mixer.

9. The magnetic separation device according to claim 8, characterized in that: The mixer has an exhaust hole on its side wall, and the support has a drain hole on its side wall that communicates with the support groove.

10. The magnetic separation device according to claim 1, characterized in that: The base plate is also provided with a splash-proof mechanism, which includes a mounting base fixedly mounted on the base plate. The mounting base has a cavity for receiving the reaction cup at the top center. Buffer cotton sheets that connect with the side walls of the reaction cup are provided on both sides of the top of the mounting base. The buffer cotton sheets are fixed by L-shaped pressure plates, and the L-shaped pressure plates are fixed to the side walls of the mounting base by bolts.

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