Portable multichannel colloidal gold detection kit

Abstract

The utility model discloses a portable multichannel colloidal gold detection kit, which comprises a shell and at least three PVC (polyvinyl chloride) plates arranged side by side along the circumferential direction of the inner side of the shell, each PVC plate is provided with a nitrocellulose membrane, a colloidal gold pad is crimped above one end of each nitrocellulose membrane, a sample pad is crimped above the colloidal gold pad, and the sample pad is connected with the shell. The other end of the nitrocellulose film is connected with absorbent paper in a pressing manner; a detection line and a quality control line are respectively arranged on the nitrocellulose film; wherein the sample pads are connected in the circumferential direction to form an integrated structure. The utility model provides a portable multi-channel colloidal gold detection kit which is provided with a plurality of detection test strips, can simultaneously detect a plurality of indexes, various virus strains or bacteria through one-time sample loading detection, realizes multi-channel rapid detection, and simultaneously reduces the detection cost.

Description

Technical Field

[0001] This utility model relates to a portable multi-channel colloidal gold detection kit. Background Technology

[0002] Currently, existing colloidal gold assay kits have the following drawbacks in terms of ease of use, portability, and multi-channel detection: The operation steps are complex, requiring precise control of sample volume, which can easily lead to errors for non-professionals. Some kits require multiple steps, such as adding samples, buffer solutions, and waiting for chromatography, increasing the difficulty of operation. They also rely on auxiliary tools, requiring the use of droppers, timers, and other equipment, further complicating the process.

[0003] Issues with size and components: Multi-component designs, such as individual buffer bottles and droppers, increase size and make them inconvenient to carry. Fragile packaging is easily damaged by compression or moisture, leading to reagent loss. Single-indicator detection is the primary focus; most kits target only a single analyte, such as a pathogen antigen. Multiplex detection requires multiple independent kits, increasing cost and time.

[0004] Existing colloidal gold reagent kits still have limitations in terms of operational standardization, environmental adaptability, and simultaneous detection of multiple indicators. Therefore, designing a portable, multi-channel detection kit is an urgent technical problem to be solved. Utility Model Content

[0005] Therefore, this invention provides a portable multi-channel colloidal gold detection kit, which has the advantages of being easy to carry and capable of simultaneous detection of multiple indicators.

[0006] To solve the above-mentioned technical problems, this utility model provides a portable multi-channel colloidal gold detection kit. The kit includes a shell and at least three PVC plates arranged side by side along the inner side of the shell. Each PVC plate is provided with a nitrocellulose film. A colloidal gold pad is pressed onto one end of the nitrocellulose film, and a sample pad is pressed onto the colloidal gold pad. Absorbent paper is pressed onto the other end of the nitrocellulose film. Detection lines and control lines are respectively provided on the nitrocellulose film.

[0007] Each of the sample pads is connected circumferentially to form an integral structure.

[0008] In one embodiment of this utility model, the outer shell is provided with a sample loading hole and multiple display windows. The sample loading hole corresponds to the sample pad, and each display window corresponds to the detection line and quality control line on the nitrocellulose membrane.

[0009] In one embodiment of this utility model, a fixing bracket is provided on the inner side of the outer shell, and a pressing strip is provided on the PVC board to fix the test strip to the inner wall of the outer shell.

[0010] In one embodiment of this utility model, the outer shell may be a cylindrical outer shell.

[0011] In one embodiment of this utility model, both ends of the outer shell are provided with covers that close the two ends of the outer shell.

[0012] In one embodiment of this utility model, the number of PVC boards is 3, 4, 5 or 6.

[0013] In one embodiment of this utility model, the PVC board consists of three strips, which are respectively used to form a first test strip for detecting classical swine fever virus, a second test strip for detecting PEVD virus strain JS2008, and a third test strip for detecting PEVD virus strain FL2013.

[0014] In one embodiment of the present invention, the colloidal gold pad of the first test strip is embedded with a colloidal gold-labeled monoclonal antibody against CSFV E2 protein, the test line is embedded with a monoclonal antibody against CSFV E2 protein, and the control line is embedded with a rabbit anti-mouse IgG antibody.

[0015] The second test strip has a colloidal gold pad containing a colloidal gold-labeled monoclonal antibody against the PEVD virus strain JS2008, the test line contains a monoclonal antibody against the PEVD virus strain JS2008, and the control line contains a rabbit anti-mouse IgG antibody.

[0016] The third test strip has a colloidal gold pad containing a colloidal gold-labeled monoclonal antibody against the PEVD virus strain JS2013. The test line contains a monoclonal antibody against the PEVD virus strain JS2013. The control line contains a rabbit anti-mouse IgG antibody.

[0017] In one embodiment of this utility model, the PVC board comprises four strips, and the test kit further includes a fourth test strip. The colloidal gold pad of the fourth test strip is embedded with the excretory-secreting (ES) antigen of Trichinella spiralis. The test line is embedded with rabbit anti-porcine IgG antibody, and the quality control line is embedded with anti-ES antibody.

[0018] In one embodiment of the present invention, the portable multichannel colloidal gold detection kit further includes a bottle containing a diluent, which can be placed inside the cavity enclosed by the outer shell.

[0019] This utility model has the following excellent effects:

[0020] This invention provides a portable multi-channel colloidal gold detection kit, which has multiple test strips. Through a single sample loading and detection, multiple indicators, multiple virus strains or bacteria can be detected simultaneously, realizing rapid multi-channel detection while reducing detection costs.

[0021] Furthermore, the cavity inside the shell formed by the outer shell of this utility model can accommodate the detection diluent, and the outer shell is cylindrical in shape, making it easy to carry. Attached Figure Description

[0022] To more clearly illustrate the embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0023] Figure 1 A three-dimensional view of a portable multi-channel colloidal gold detection kit provided by this utility model;

[0024] Figure 2 This invention provides a front view of a portable multichannel colloidal gold detection kit;

[0025] Figure 3 For the present utility model Figure 2 BB-direction cross-section;

[0026] Figure 4 This is a schematic diagram of the internal structure of a portable multichannel colloidal gold detection kit according to the present invention;

[0027] Figure 5 This is a front view of the internal structure of a portable multichannel colloidal gold detection kit according to the present invention.

[0028] Figure 6 For the present utility model Figure 5 BB-direction cross-section;

[0029] In the diagram: 100 - outer casing; 110 - display window; 120 - sample loading port; 130 - cover; 200 - test strip; 210 - sample pad; 220 - colloidal gold pad; 230 - nitrocellulose film; 240 - absorbent paper; 250 - PVC board; 300 - mounting bracket. Detailed Implementation

[0030] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0031] like Figures 1-6 As shown, this utility model provides a portable multi-channel colloidal gold detection kit. The kit includes a shell 100 and at least three PVC plates 250 arranged circumferentially along the inner side of the shell 100. During actual testing, the number of PVC plates 250 is set according to the indicators and contents to be detected. For example, to detect three indicators, three PVC plates 250 can be set, forming a triangular prism shape; to detect four indicators, four PVC plates 250 can be set, forming a cuboid shape; to detect five indicators, five PVC plates 250 can be set, forming a pentagonal prism shape; and to detect six indicators, six PVC plates 250 can be set, forming a hexagonal prism shape. This allows for the corresponding setting of colloidal gold test strips 200 for detecting different indicators on each PVC plate 250.

[0032] Each PVC board 250 has a nitrocellulose film 230, with a colloidal gold pad 220 pressed onto one end of the nitrocellulose film, a sample pad 210 pressed onto the colloidal gold pad 220, and absorbent paper 240 pressed onto the other end of the nitrocellulose film 230. The nitrocellulose film 230 has detection lines and quality control lines respectively. Each sample pad 210 is connected circumferentially to form an integral structure, so that the sample to be tested can be loaded at one time and multiple test strips 200 can be tested at the same time, thereby realizing multiple index tests with one loading, which is more convenient and faster, and can reduce testing costs.

[0033] The colloidal gold detection kit of this invention has a sample loading port 120 and multiple display windows 110 on its outer shell 100. The outer shell 100 encloses the internal test strips 200. The sample loading port 120 of the outer shell 100 corresponds to the sample pad 210, and each display window 110 corresponds to the detection line and control line on the nitrocellulose film 230 of each test strip 200. Therefore, the sample loading port 120 can be set at any position corresponding to the sample pad 210, allowing multiple test strips 200 to be tested with a single sample loading.

[0034] Preferably, the inner side of the reagent kit shell 100 is provided with a fixing bracket 300, which is provided with a clamping strip corresponding to the PVC plate 250 to fix the test strip 200 to the inner side wall of the shell 100 to prevent the test strip from sliding or falling off. The shell 100 can be set as a cylindrical shell 100 or adapted to the shape formed by the internal test strip to form a triangular prism, cuboid or other shapes.

[0035] The portable multichannel colloidal gold detection kit of this invention has caps 130 at both ends of the outer shell 100, which seal both ends of the outer shell 100, forming a sealed cavity inside the shell. This sealed cavity can accommodate a bottle containing diluent. In this way, when using the diluent, the caps 130 can be opened to take out the bottle of diluent, making it convenient to carry the diluent without additional packaging.

[0036] As an alternative implementation, the number of PVC panels 250 inside the outer shell 100 of the portable multichannel colloidal gold detection kit of this invention is 3, 4, 5 or 6.

[0037] For example, when PVC board 250 is divided into 3 strips, it can be used to form a first test strip 200 for detecting swine fever virus, a second test strip 200 for detecting PEVD virus strain JS2008, and a third test strip 200 for detecting PEVD virus strain FL2013.

[0038] Among them, the first test strip 200 and colloidal gold pad 220 are embedded with colloidal gold-labeled monoclonal antibody against CSFV E2 protein, the test line is embedded with monoclonal antibody against CSFV E2 protein, and the quality control line is embedded with rabbit anti-mouse IgG antibody.

[0039] The second test strip 200 and colloidal gold pad 220 contain colloidal gold-labeled monoclonal antibodies against the PEVD virus strain JS2008. The test line contains monoclonal antibodies against the PEVD virus strain JS2008, and the quality control line contains rabbit anti-mouse IgG antibodies.

[0040] The third test strip 200 contains a colloidal gold pad 220 embedded with a colloidal gold-labeled monoclonal antibody against the PEVD virus strain JS2013. The detection line contains a monoclonal antibody against the PEVD virus strain JS2013, and the control line contains rabbit anti-mouse IgG antibody. When all three test strips 200 are used, this test kit can simultaneously detect PEVD virus strain JS2008, CSFV, and PEVD virus strain JS2013.

[0041] The PVC plates 250 are divided into four strips. In addition to the three colloidal gold test strips 200 mentioned above, the detection kit of this invention also includes a fourth test strip 200. The colloidal gold pad 220 of the fourth test strip 200 is embedded with the excretory-secreting (ES) antigen of *Trichinella spiralis*, the detection line is embedded with rabbit anti-porcine IgG antibody, and the control line is embedded with anti-ES antibody. When four PVC plates 250 are used, in addition to detecting the above three strains, it can further detect porcine trichinosis.

[0042] Preferably, the number of PVC plates 250 inside the shell can be set to 6, and test strips 200, 3, 4, 5, or 6 can be placed on the 6 PVC plates 250 as needed. The outer shell 100 of the test kit is also set to a cylindrical shape.

[0043] When using this invention, open the cover 130, take out the diluent, pour the diluent into the sample to be tested, and after dilution, pour the diluted solution into the sample loading hole 120. The solution flows through the sample pad 210 onto the colloidal gold pad 220 of different test strips 200, and then through their respective nitrocellulose films 230 for detection and display on the detection line and quality control line to complete the test results.

[0044] Although the present invention has been described in detail above with general descriptions and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A portable multichannel colloidal gold detection kit, characterized in that, The kit includes an outer shell and at least three PVC plates arranged circumferentially along the inner side of the outer shell. Each PVC plate is provided with a nitrocellulose film. A colloidal gold pad is pressed onto one end of the nitrocellulose film, and a sample pad is pressed onto the colloidal gold pad. Absorbent paper is pressed onto the other end of the nitrocellulose film. Detection lines and control lines are respectively provided on the nitrocellulose film. Each of the sample pads is connected circumferentially to form an integral structure.

2. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, The outer shell has a sample loading port and multiple display windows. The sample loading port corresponds to the sample pad, and each display window corresponds to the detection line and quality control line on the nitrocellulose membrane.

3. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, The inner side of the outer shell is provided with a fixing bracket, and the bracket is provided with a clamping strip corresponding to the PVC board, so as to fix the PVC board to the inner wall of the outer shell.

4. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, The outer shell can be configured as a cylindrical shell.

5. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, Both ends of the outer shell are provided with covers that seal both ends of the outer shell.

6. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, The number of PVC boards is 3, 4, 5 or 6.

7. The portable multichannel colloidal gold detection kit as described in claim 1, characterized in that, The PVC board consists of three strips, which are used to form a first test strip for detecting classical swine fever virus, a second test strip for detecting PEVD virus strain JS2008, and a third test strip for detecting PEVD virus strain FL2013. Each test strip includes a nitrocellulose film on a PVC board, a colloidal gold pad pressed onto one end of the nitrocellulose film, a sample pad pressed onto the colloidal gold pad, and absorbent paper pressed onto the other end of the nitrocellulose film. The nitrocellulose film is provided with a detection line and a quality control line.

8. The portable multichannel colloidal gold detection kit as described in claim 7, characterized in that, The first test strip has a colloidal gold pad containing a colloidal gold-labeled monoclonal antibody against CSFV E2 protein, the test line contains a monoclonal antibody against CSFV E2 protein, and the control line contains a rabbit anti-mouse IgG antibody. The second test strip has a colloidal gold pad containing a colloidal gold-labeled monoclonal antibody against the PEVD virus strain JS2008, the test line contains a monoclonal antibody against the PEVD virus strain JS2008, and the control line contains a rabbit anti-mouse IgG antibody. The third test strip has a colloidal gold pad containing a colloidal gold-labeled monoclonal antibody against the PEVD virus strain JS2013. The test line contains a monoclonal antibody against the PEVD virus strain JS2013. The control line contains a rabbit anti-mouse IgG antibody.

9. The portable multichannel colloidal gold detection kit as described in claim 8, characterized in that, The PVC board comprises four strips, and the test kit further includes a fourth test strip. The colloidal gold pad of the fourth test strip is embedded with the excretory-secreting (ES) antigen of Trichinella spiralis. The test line is embedded with rabbit anti-porcine IgG antibody, and the quality control line is embedded with anti-ES antibody.

10. The portable multi-channel colloidal gold detection test kit according to claim 1, wherein It also includes a bottle containing a diluent, which can be placed within the cavity enclosed by the outer casing.

Images