High-throughput filter membrane screening plate

Abstract

The utility model discloses a high-throughput filter membrane screening plate, relates to a biological medicine research and development device, and is used for testing the filtering effects of different filter membranes on biological macromolecular medicines in a high-throughput manner. The device comprises a frame, battens and a cover plate, each batten is provided with a concave hole, the middle of each hole is provided with a protruding clamping seat, and the device is further provided with a clamping sleeve. According to the utility model, the filtering effects of different filter membranes on biological macromolecular drugs can be tested in a multi-channel and high-throughput manner, only a trace amount of macromolecular drug samples can be tested and analyzed, and the number of the battens can be flexibly selected according to the number of the filter membranes to be tested, so that the screening efficiency of the filter membranes is greatly improved, and raw materials are saved.

Description

Technical Field

[0001] This utility model relates to a biomedical research and development device, and more specifically, to a high-throughput filter membrane screening plate. Background Technology

[0002] Biological macromolecules are a class of biological macromolecules produced using modern biotechnology for the diagnosis, treatment, and prevention of diseases. These include monoclonal antibodies, recombinant proteins, peptides, enzymes, and cytokines. These drugs are characterized by strong targeting, high specificity, low toxicity, and high sensitivity, demonstrating significant efficacy in the treatment of various major diseases, including malignant tumors, immune system diseases, metabolic diseases, genetic diseases, and infectious diseases.

[0003] The laboratory research and development phase of biopharmaceuticals includes drug screening, manufacturing process development, quality studies, and pharmacological and toxicological studies. The manufacturing process of biopharmaceuticals can be divided into three stages: fermentation (upstream process), purification (downstream process), and formulation. Membrane technologies, including depth filtration membranes, membrane chromatography media and equipment, virus filtration membranes, and ultrafiltration / percolation membranes for protein concentration and buffer replacement, have important applications in different stages of the downstream separation and purification process of biopharmaceuticals, including clarification, capture, purification and refining, and pre-formulation units.

[0004] In the development of membrane separation and purification processes for biopharmaceutical drugs, selecting a separation membrane with suitable pore size, material, and surface physicochemical properties is crucial. Due to the wide variety of membrane material brands and specifications available on the market, traditional screening methods are time-consuming. Therefore, there is an urgent need for a high-throughput membrane material screening device to improve the development efficiency of membrane separation and purification processes for macromolecular drugs. Summary of the Invention

[0005] This invention provides a high-throughput filter membrane screening plate for high-throughput testing of the filtration effect of different filter membranes on biopharmaceutical drugs.

[0006] The technical solution of this utility model is:

[0007] A high-throughput filter membrane screening plate, such as Figure 1 , Figure 2 As shown, the screen includes a frame 1, slats 2, and a cover plate 3. The slats 2 can be fitted onto the frame 1, and one to twelve slats 2 can be fitted onto one frame 1. Optionally, the screen also includes two sealing strips 4, which are used to fix the slats 2 onto the frame 1.

[0008] Each slat 2 has 4 to 8 recessed holes 5, and each hole 5 has a protruding seat 6 in the middle waist for placing the filter membrane 7; each hole 5 is also equipped with a sleeve 8 for fixing the filter membrane 7.

[0009] The material of the slats 2 and the sleeves 8 is polystyrene, polypropylene or polyethylene.

[0010] The filter membrane 7 can be made of various pore sizes or molecular weight cutoffs, such as cellulose membranes, regenerated cellulose membranes, nylon membranes, polyethylene membranes, etc.

[0011] The method of using this invention is as follows: Based on the number of filter membranes 7 to be tested, select an appropriate number of strips 2, fit them onto the frame 1, and secure them with seals 4 on both sides. Place the appropriately sized filter membranes 7 into the pores 5, use the retainer 8 to push the filter membranes 7 into the pores 5, and fix the filter membranes 7 between the retainer 6 and the bottom of the retainer 8. Place the assembled screening plate on a fully automated liquid workstation (or automated pipetting system), add a solution containing macromolecular biopharmaceuticals to the pores 5, then cover with the cover plate 3, and place it on a centrifuge for centrifugation. After centrifugation, open the cover plate 3 and analyze the solution at the bottom of each pore 5 to evaluate the filtration effect of the filter membrane under test.

[0012] The advantages of this invention are that it enables multi-channel, high-throughput testing of the filtration effects of different filter membranes on biopharmaceutical drugs, requires only a small amount of macromolecular drug sample for testing and analysis, and allows for flexible selection of the number of strips based on the number of filter membranes to be tested, thereby greatly improving the efficiency of filter membrane screening and saving raw materials. Attached Figure Description

[0013] Figure 1 : A longitudinal cross-sectional view of a high-throughput filter membrane screening plate.

[0014] Figure 2 A top view of a high-throughput filter membrane screening plate after the cover is opened.

[0015] Reference numerals: 1-frame, 2-slats, 3-cover plate, 4-seal, 5-cavity, 6-slot, 7-filter membrane, 8-clamp. Detailed Implementation

[0016] Example 1: Structure of a high-throughput filter membrane screening plate

[0017] A high-throughput filter membrane screening plate, such as Figure 1 , Figure 2 As shown, the screen includes a frame 1, slats 2, and a cover plate 3. The slats 2 can be fitted onto the frame 1, and one frame 1 can accommodate four slats 2. The screen also includes two sealing strips 4, which are used to fix the slats 2 onto the frame 1.

[0018] Each slat 2 has 4 recessed holes 5, and each hole 5 has a protruding seat 6 in the middle for placing the filter membrane 7; each hole 5 is also equipped with a sleeve 8 for fixing the filter membrane 7.

[0019] The material of the slats 2 and the sleeves 8 is polypropylene.

[0020] The filter membrane 7 can be made of various pore sizes or molecular weight cutoffs, such as cellulose membranes, regenerated cellulose membranes, nylon membranes, polyethylene membranes, etc.

[0021] Example 2: A method for using a high-throughput filter membrane screening plate

[0022] The method of using this utility model is as follows:

[0023] Based on the number of filter membranes 7 to be tested, select an appropriate number of strips 2, fit them onto the frame 1, and secure them with seals 4 on both sides. Place the appropriately sized filter membranes 7 into the pores 5, use the retainer 8 to push the filter membranes 7 into the pores 5, and fix the filter membranes 7 between the retainer 6 and the bottom of the retainer 8. Place the assembled screening plate on a fully automated liquid workstation (or automated pipetting system), add the solution containing macromolecular biopharmaceuticals to the pores 5, then cover with the cover plate 3, place it on a centrifuge for centrifugation, and after centrifugation, open the cover plate 3 and analyze the solution at the bottom of each pore 5 to evaluate the filtration effect of the filter membranes to be tested.

Claims

1. A high-throughput filter membrane screening plate, characterized in that, Includes a frame (1), slats (2) and a cover plate (3). The slats (2) can be fitted onto the frame (1). Each slat (2) has a recessed hole (5). Each hole (5) has a protruding seat (6) in the middle waist for placing the filter membrane (7). Each hole (5) is also equipped with a sleeve (8) for fixing the filter membrane (7).

2. The high-throughput filter membrane screening plate according to claim 1, characterized in that, The number of holes (5) on each slat (2) is 4 to 8, and the number of slats (2) that can be fitted into a frame (1) is 1 to 12.

3. The high-throughput filter membrane screening plate according to claim 1, characterized in that, It also includes two seals (4) for securing the strips (2) to the frame (1).

4. The high-throughput filter membrane screening plate according to claim 1, characterized in that, The material of the slats (2) and the sleeves (8) is polystyrene, polypropylene or polyethylene.

5. The high-throughput filter membrane screening plate according to claim 1, characterized in that, The filter membrane (7) is a cellulose membrane, a regenerated cellulose membrane, a nylon membrane, or a polyethylene membrane.

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