Tobacco production raw material DNA extraction test tube

Abstract

The utility model relates to the technical field of extraction test tubes, in particular to a tobacco raw material DNA extraction test tube which comprises an ep test tube shell, a liquid storage shell is inserted into the inner wall of a fixed sleeve, so that an electric control pressurizing spray head and a connecting tube are respectively inserted into an inner cavity of a connecting sleeve, a flexible sealing block is jacked open, and after a controller switch is turned on, DNA extraction is performed. The micro liquid outlet pump starts to operate to suck purified water in the inner cavity of the liquid storage shell, then, the electric control pressurizing spray head sprays out high-pressure gas under the action of the controller to flush the inner cavity of the ep test tube shell, and meanwhile, the micro liquid pump also starts to work to pump flushing waste liquid out of the inner cavity of the ep test tube shell and discharge the flushing waste liquid into a waste liquid cavity of the liquid storage shell; the locking buckle is fixed to the outer wall of the ep test tube shell, stability is better, meanwhile, the hinge is not prone to breakage, the steel ball is fixed through the connecting steel rope, the steel ball is not prone to being lost, the ep test tube shell and the ep test tube cover which are made of stainless steel are firmer, and the tube wall or the tube cover cannot be broken by the steel ball.

Description

Technical Field

[0001] This utility model relates to the field of extraction test tube technology, specifically a test tube for extracting DNA from tobacco raw materials. Background Technology

[0002] DNA molecular marker identification testing of tobacco raw materials is mainly used for quantitative and qualitative identification of tobacco and non-tobacco products, types, and varieties. High-quality genomic DNA from the samples is a prerequisite for ensuring the stability and accuracy of the above identification test results. Before extracting DNA from tobacco raw materials, the sample tissue needs to be weighed. Then, the leaf tissue is placed into an EP tube with thickened plastic walls, and finally, a ground steel ball is placed in it. The tube is then placed below the surface of liquid nitrogen for freezing. The EP tube is then placed in the sample holder of an automated crusher. The rapid reciprocating motion of the automated crusher's swing arm, along with the inertia of the steel ball, breaks up the liquid nitrogen-cooled tobacco raw material sample.

[0003] Because the EP tube is made of plastic, it becomes brittle after being frozen in liquid nitrogen. During the high-speed reciprocating motion of the automated crusher's arm, the steel balls often break through the tube wall or cap (the cap is connected to the plastic), causing sample powder to leak and contaminate the sample holder cavity wall and control panel of the automated crusher. Furthermore, due to the high price of the steel balls (made of titanium-magnesium alloy), they are reusable. Therefore, after DNA extraction, the steel balls must be poured out of the test tube, cleaned, and recycled. Because the steel balls are small and often adhere to the bottom or wall of the test tube due to extraction residue, they are difficult to pour out, and often are accidentally discarded.

[0004] Furthermore, after the pulverization and extraction, the powder adhering to the inner wall of the EP tube needs to be cleaned in time. However, the existing brushes used to clean the inside of the EP tube are limited by the material and shape of the brushes, and sometimes it is difficult to completely fit the shape of the inner wall of the EP tube. As a result, some powder in corners or gaps cannot be effectively removed, which is time-consuming and laborious.

[0005] Therefore, a DNA extraction tube for tobacco raw materials is needed to improve the above-mentioned problems. Utility Model Content

[0006] The purpose of this invention is to provide a DNA extraction test tube for tobacco raw materials to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A DNA extraction test tube for tobacco raw materials includes an EP test tube shell with graduations on its outer wall. An EP test tube cap is hinged to one end of the EP test tube shell. A protrusion is provided at one end of the EP test tube cap, and a locking buckle is installed on the outer wall of the protrusion. A connecting steel rope is provided on the inner wall of the EP test tube cap, and a steel ball is installed at one end of the connecting steel rope. A cleaning component is embedded in the outer wall of the EP test tube cap.

[0009] As a preferred embodiment of this utility model, the cleaning assembly includes a mounting plate, which is embedded in the outer wall of the EP test tube cap. Connecting sleeves are symmetrically arranged on the outer wall of the mounting plate, and flexible sealing blocks are arranged on the inner wall of the connecting sleeves. A fixing sleeve is embedded in the outer wall of the mounting plate.

[0010] As a preferred embodiment of this utility model, a liquid storage shell is inserted and installed on the outer wall of the fixed sleeve, a partition is provided on the inner wall of the liquid storage shell, a micro liquid pump is provided on one side of the partition and on the outer wall of the liquid storage shell, and an electrically controlled booster nozzle is installed at the outlet of the micro liquid pump.

[0011] As a preferred embodiment of this utility model, a micro pump is provided on the other side of the partition and on the outer wall of the liquid storage shell, the inlet of the micro pump is provided with a connecting pipe, and a controller is provided on the outer wall of the liquid storage shell.

[0012] As a preferred embodiment of this utility model, the locking buckle includes a male locking buckle and a female locking buckle. The female locking buckle is installed on the outer wall of the protrusion, and the male locking buckle is installed on the outer wall of the EP test tube shell. Both the EP test tube shell and the EP test tube cap are made of stainless steel.

[0013] As a preferred embodiment of this utility model, the controller is connected to a micro liquid dispensing pump, an electrically controlled booster nozzle, and a micro liquid pump via wires, and the connection method is an electrical connection.

[0014] As a preferred embodiment of this utility model, the protrusion is located on one side of the ep test tube shell, and the connecting steel rope is located on one side of the mounting plate.

[0015] As a preferred embodiment of this utility model, the connecting steel rope is located in the inner cavity of the EP test tube shell, and the steel ball is located at the bottom of the inner cavity of the EP test tube shell.

[0016] As a preferred embodiment of this utility model, the connecting sleeve is provided in two sets and is located on the outer wall of the mounting plate respectively, and the port of the connecting sleeve is flush with the outer wall of the mounting plate.

[0017] As a preferred embodiment of this utility model, the partition divides the inner cavity of the liquid storage shell into a clean liquid cavity and a waste liquid cavity, the micro liquid outlet pump is located on one side of the clean liquid cavity, and the micro liquid pump is located on one side of the waste liquid cavity.

[0018] Compared with the prior art, the beneficial effects of this utility model are:

[0019] 1. In this utility model, a stainless steel EP test tube shell and EP test tube cap are used in the DNA extraction test tube for tobacco raw materials. After the EP test tube cap is put on, the locking buckle is fixed to the outer wall of the EP test tube shell, which has better stability and the hinge is not easy to break. The connecting steel rope fixes the steel ball, and the steel ball is not easy to be lost. The stainless steel EP test tube shell and EP test tube cap are more robust and will not be broken by the steel ball. This helps to solve the problem that the plastic EP tube becomes hard and brittle after being frozen with liquid nitrogen, and the EP test tube wall breaks, the cap hinge breaks, and the steel ball is difficult to remove and easy to lose during the high-speed reciprocating motion of the swing arm of the automated crusher.

[0020] 2. In this utility model, a cleaning component is used in the DNA extraction test tube for tobacco raw materials. The liquid storage shell is inserted into the inner wall of the fixed sleeve, and the electrically controlled pressurized nozzle and the connecting tube are respectively inserted into the inner cavity of the connecting sleeve. After the flexible sealing block is opened and the controller switch is turned on, the micro liquid pump starts to operate, drawing in pure water from the inner cavity of the liquid storage shell. Then, the electrically controlled pressurized nozzle sprays high-pressure gas under the action of the controller to rinse the inner cavity of the EP test tube shell. At the same time, the micro liquid pump also starts to work, drawing the rinsing waste liquid from the inner cavity of the EP test tube shell and discharging it into the waste liquid cavity of the liquid storage shell. This helps to solve the problem of timely cleaning of powder adhering to the inner wall of the EP tube. However, existing brushes are limited by their material and shape, making it difficult to completely adhere to the inner wall of the tube, resulting in incomplete removal of powder from corners or crevices, which is time-consuming and laborious. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a side view of the structure of this utility model;

[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of the ep test tube shell of this utility model;

[0024] Figure 4 This is a side view of the structure of this utility model;

[0025] Figure 5 This utility model Figure 4 An enlarged schematic diagram of the A structure.

[0026] In the diagram: 1. EP test tube shell; 2. Scale; 3. Hinge; 4. EP test tube cap; 5. Protrusion; 6. Locking buckle; 61. Male locking buckle; 62. Female locking buckle; 7. Connecting steel rope; 8. Steel ball; 9. Cleaning assembly; 901. Mounting plate; 902. Connecting sleeve; 903. Flexible sealing block; 904. Fixing sleeve; 905. Liquid storage shell; 906. Partition plate; 907. Miniature liquid dispensing pump; 908. Electrically controlled booster nozzle; 909. Miniature liquid extraction pump; 910. Connecting pipe; 911. Controller. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0028] For examples, please refer to Figure 1-5 This utility model provides a technical solution:

[0029] A DNA extraction test tube for tobacco raw materials includes an EP test tube shell 1, with graduations 2 on the outer wall of the EP test tube shell 1, an EP test tube cap 4 mounted on one end of the EP test tube shell 1 via a hinge 3, a protrusion 5 on one end of the EP test tube cap 4, a locking buckle 6 mounted on the outer wall of the protrusion 5, a connecting steel rope 7 on the inner wall of the EP test tube cap 4, a steel ball 8 mounted on one end of the connecting steel rope 7, and a cleaning component 9 embedded in the outer wall of the EP test tube cap 4.

[0030] In this embodiment, please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 The cleaning component 9 includes a mounting plate 901, which is embedded in the outer wall of the ep test tube cap 4. Connecting sleeves 902 are symmetrically arranged on the outer wall of the mounting plate 901. Flexible sealing blocks 903 are arranged on the inner wall of the connecting sleeves 902. A fixing sleeve 904 is embedded in the outer wall of the mounting plate 901. A liquid storage shell 905 is inserted and removed from the outer wall of the fixing sleeve 904. A partition 906 is arranged on the inner wall of the liquid storage shell 905. A micro liquid outlet pump 907 is arranged on one side of the partition 906 and on the outer wall of the liquid storage shell 905. An electrically controlled booster nozzle 908 is installed at the outlet of the micro liquid outlet pump 907. A micro liquid extraction pump 909 is arranged on the other side of the partition 906 and on the outer wall of the liquid storage shell 905. A connecting pipe 910 is arranged at the inlet of the micro liquid extraction pump 909. A controller 911 is arranged on the outer wall of the liquid storage shell 905.

[0031] Furthermore, the locking buckle 6 includes a male locking buckle 61 and a female locking buckle 62. The female locking buckle 62 is installed on the outer wall of the protrusion 5, and the male locking buckle 61 is installed on the outer wall of the ep test tube shell 1.

[0032] Furthermore, both the EP test tube housing 1 and the EP test tube cap 4 are made of stainless steel. The controller 911 is electrically connected to the micro-dispensing pump 907, the electrically controlled booster nozzle 908, and the micro-suction pump 909 via wires, thus energizing the device. This, in turn, causes the controller 911 to control the operation of the micro-dispensing pump 907, the electrically controlled booster nozzle 908, and the micro-suction pump 909. The protrusion 5 is located on one side of the EP test tube housing 1, and the connecting steel cable 7 is located at the... On one side of the mounting plate 901, the connecting steel rope 7 is located in the inner cavity of the ep test tube shell 1, the steel ball 8 is located at the bottom of the inner cavity of the ep test tube shell 1, two sets of connecting sleeves 902 are provided and are located on the outer wall of the mounting plate 901 respectively, and the port of the connecting sleeve 902 is flush with the outer wall of the mounting plate 901. The partition 906 divides the inner cavity of the liquid storage shell 905 into a clean liquid cavity and a waste liquid cavity. The micro liquid discharge pump 907 is located on one side of the clean liquid cavity, and the micro liquid extraction pump 909 is located on one side of the waste liquid cavity.

[0033] The working process of this utility model is as follows: When using the DNA extraction test tube for tobacco raw materials designed in this scheme, a scale 2 is set on the outer wall of the EP test tube shell 1. An EP test tube cap 4 is installed at one end of the EP test tube shell 1 via a hinge 3. Both the EP test tube shell 1 and the EP test tube cap 4 are made of stainless steel. When the EP test tube shell 1 is frozen and then subjected to the high-speed reciprocating motion of the swing arm of the automated crusher, the steel ball 8 often breaks the tube wall or the cap. The stainless steel EP test tube shell 1 and EP test tube cap 4 are more robust. A protrusion 5 is set at one end of the EP test tube cap 4, and a locking buckle 6 is installed on the outer wall of the protrusion 5. When the EP test tube cap 4 is closed, the locking buckle 6 is fixed to the outer wall of the EP test tube shell 1, which improves stability and makes the hinge 3 less likely to break. In addition, a connecting steel rope 7 is set on the inner wall of the EP test tube cap 4. With a steel ball 8 installed at one end of the connecting steel rope 7, when the crushed raw materials are poured out after use, the connecting steel rope 7 fixes the steel ball 8, making it less likely to be lost or stuck. This helps to solve the problems of EP tube walls breaking, cap hinge breaking, steel ball being difficult to remove and easily lost when the plastic EP tube becomes hard and brittle after being frozen with liquid nitrogen and during the high-speed reciprocating motion of the swing arm of the automated crusher.

[0034] The inner cavity of the liquid storage shell 905 is divided into a clean liquid chamber and a waste liquid chamber by a partition 906. A micro-discharge pump 907 is located on one side of the clean liquid chamber, and a micro-pump 909 is located on the side of the waste liquid chamber. Under the action of these actions, purified water is injected into the clean liquid chamber. Meanwhile, a partition 906 is provided on the inner wall of the liquid storage shell 905, and a micro-discharge pump 907 is located on one side of the partition 906 and on the outer wall of the liquid storage shell 905. The micro-discharge pump 907... An electrically controlled booster nozzle 908 is installed at the liquid inlet. A miniature liquid pump 909 is installed on the other side of the partition 906, located on the outer wall of the liquid storage housing 905. The inlet of the miniature liquid pump 909 is connected to a connecting pipe 910. Under the action of this pipe, the liquid storage housing 905 is inserted into the inner wall of the fixing sleeve 904, causing the electrically controlled booster nozzle 908 to be inserted into the inner cavity of the connecting sleeve 902 on one side. This causes the electrically controlled booster nozzle 908 to push open the flexible sealing block 903. The connecting tube 910 is also inserted into the inner cavity of the connecting sleeve 902 on the other side, which causes the connecting tube 910 to push open the flexible sealing block 903. Then, the switch of the controller 911 is turned on, which causes the controller 911 to control the micro liquid pump 907 to operate, which draws in the pure water in the inner cavity of the liquid storage shell 905. Then, the controller 911 controls the electric pressure boosting nozzle 908 to operate, which causes the electric pressure boosting nozzle 908 to spray high-pressure gas to rinse the inner cavity of the EP test tube shell 1. At the same time, the controller 911 controls the micro liquid extraction pump 909 to operate, which causes the micro liquid extraction pump 909 to extract the rinsing waste liquid in the inner cavity of the EP test tube shell 1. Then, the micro liquid extraction pump 909 discharges the waste liquid into the waste liquid cavity of the liquid storage shell 905. This helps to solve the problem of timely cleaning of powder adhering to the inner wall of the EP tube. However, due to the limitations of the material and shape, the existing brush is difficult to completely fit the inner wall of the tube, resulting in incomplete cleaning of powder in corners or gaps, which is time-consuming and laborious.

[0035] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A DNA extraction test tube for tobacco raw materials, comprising an ep test tube shell (1), characterized in that: The outer wall of the EP test tube housing (1) is provided with a scale (2). One end of the EP test tube housing (1) is fitted with an EP test tube cap (4) via a hinge (3). One end of the EP test tube cap (4) is provided with a protrusion (5). A locking buckle (6) is installed on the outer wall of the protrusion (5). A connecting steel rope (7) is provided on the inner wall of the EP test tube cap (4). A steel ball (8) is installed on one end of the connecting steel rope (7). A cleaning component (9) is embedded in the outer wall of the EP test tube cap (4).

2. The DNA extraction test tube for tobacco raw materials according to claim 1, characterized in that: The cleaning assembly (9) includes a mounting plate (901), which is embedded in the outer wall of the ep test tube cap (4). Connecting sleeves (902) are symmetrically arranged on the outer wall of the mounting plate (901). A flexible sealing block (903) is provided on the inner wall of the connecting sleeve (902). A fixing sleeve (904) is embedded in the outer wall of the mounting plate (901).

3. The DNA extraction test tube for tobacco raw materials according to claim 2, characterized in that: A liquid storage shell (905) is inserted and installed on the outer wall of the fixed sleeve (904). A partition (906) is provided on the inner wall of the liquid storage shell (905). A micro liquid pump (907) is provided on one side of the partition (906) and on the outer wall of the liquid storage shell (905). An electrically controlled booster nozzle (908) is installed at the outlet of the micro liquid pump (907).

4. The DNA extraction test tube for tobacco raw materials according to claim 3, characterized in that: A micro pump (909) is provided on the other side of the partition (906) and on the outer wall of the liquid storage shell (905). The inlet of the micro pump (909) is provided with a connecting pipe (910), and a controller (911) is provided on the outer wall of the liquid storage shell (905).

5. The DNA extraction test tube for tobacco raw materials according to claim 1, characterized in that: The locking buckle (6) includes a male locking buckle (61) and a female locking buckle (62). The female locking buckle (62) is installed on the outer wall of the protrusion (5), and the male locking buckle (61) is installed on the outer wall of the EP test tube shell (1). The EP test tube shell (1) and the EP test tube cap (4) are both made of stainless steel.

6. The DNA extraction test tube for tobacco raw materials according to claim 4, characterized in that: The controller (911) is connected to a micro liquid dispensing pump (907), an electrically controlled booster nozzle (908), and a micro liquid extraction pump (909) via wires, and the connection method is electrical connection.

7. The DNA extraction test tube for tobacco raw materials according to claim 2, characterized in that: The protrusion (5) is located on one side of the ep test tube housing (1), and the connecting steel rope (7) is located on one side of the mounting plate (901).

8. The DNA extraction test tube for tobacco raw materials according to claim 1, characterized in that: The connecting steel rope (7) is located in the inner cavity of the ep test tube shell (1), and the steel ball (8) is located at the bottom of the inner cavity of the ep test tube shell (1).

9. A test tube for extracting DNA from tobacco raw materials according to claim 2, characterized in that: Two sets of connecting sleeves (902) are provided and are respectively located on the outer wall of the mounting plate (901), and the port of the connecting sleeve (902) is flush with the outer wall of the mounting plate (901).

10. A DNA extraction test tube for tobacco raw materials according to claim 4, characterized in that: The partition (906) divides the inner cavity of the liquid storage shell (905) into a clean liquid cavity and a waste liquid cavity. The micro liquid outlet pump (907) is located on one side of the clean liquid cavity, and the micro liquid pump (909) is located on one side of the waste liquid cavity.

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