Device for preparing crude heparin sodium from small intestinal mucosa

Abstract

The utility model discloses a device for preparing crude heparin sodium from small intestinal mucosa, which comprises an integrated frame, a PLC (programmable logic controller) is mounted on one side of the integrated frame, and a primary enzymolysis tank and a secondary enzymolysis tank are respectively fixed at two ends inside the integrated frame. When the device runs, treated small intestine mucous membrane slurry is added into the primary enzymolysis tank through the corrosion-resistant pump on the feeding pipe fitting, and the external temperature control heating sleeves are fixed on the outer walls of the primary enzymolysis tank and the secondary enzymolysis tank; a water bath heating cavity which is provided with an electric heating wire and a temperature sensor and is arranged in the side wall of the external temperature control heating sleeve is matched, the temperature control heating effect on the primary enzymolysis tank and the secondary enzymolysis tank can be achieved, and then the timing control effect of the PLC is matched, so that after the temperature control timing reaction of the materials in the primary enzymolysis tank is achieved, the reaction time is shortened; and the material is automatically transferred into the secondary enzymolysis tank through a corrosion-resistant pump on the material transferring pipe fitting to complete secondary temperature control timing reaction, so that the device realizes continuous two-time enzymolysis treatment.

Description

Technical Field

[0001] This utility model relates to the field of crude heparin sodium preparation technology, specifically an apparatus for preparing crude heparin sodium from the small intestinal mucosa. Background Technology

[0002] Heparin sodium is a highly effective anticoagulant that exerts a strong anticoagulant effect both in vivo and in vitro. The small intestinal mucosa of livestock such as pigs and cattle is the main source of heparin sodium. By developing the economic value of the small intestinal mucosa, the comprehensive utilization rate of resources is improved, and the maximum utilization of resources is achieved. While meeting market demand, it promotes the development of related industries towards a green, environmentally friendly and sustainable direction.

[0003] Current devices for producing crude heparin sodium from small intestinal mucosa via enzymatic hydrolysis produce a strong odor after drying, which affects the work of staff and fails to meet actual production needs. Furthermore, these devices only provide a single agitation-assisted enzymatic hydrolysis function; they cannot support two consecutive enzymatic hydrolysis processes or automatically control reaction time and temperature. Therefore, we propose a novel device for producing crude heparin sodium from small intestinal mucosa, which enhances functionality, optimizes performance, and improves reaction efficiency. Utility Model Content

[0004] The purpose of this invention is to provide an apparatus for producing crude heparin sodium from the small intestinal mucosa, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an apparatus for producing crude heparin sodium from small intestinal mucosa, comprising an integrated frame, a PLC controller installed on one side of the integrated frame, a primary enzymatic hydrolysis tank and a secondary enzymatic hydrolysis tank fixed at both ends inside the integrated frame, a transfer pipe connecting the primary and secondary enzymatic hydrolysis tanks, a feed pipe installed at the top of the primary enzymatic hydrolysis tank, a corrosion-resistant pump installed on both the feed pipe and the transfer pipe, a top cover fitted onto the top of both the primary and secondary enzymatic hydrolysis tanks, a servo motor installed on the top of the top cover, a stirring shaft connected to the output end of the servo motor, a guide fan blade installed at the top of the stirring shaft, and an activated carbon deodorizing cylinder matching the guide fan blades connected to the bottom of the top cover with uniform threads, an external temperature-controlled heating jacket fitted onto both the primary and secondary enzymatic hydrolysis tanks, a water bath heating chamber provided inside the side wall of the external temperature-controlled heating jacket, and a heating wire and a temperature sensor installed inside the water bath heating chamber.

[0006] Preferably, the bottom of the secondary enzymatic hydrolysis tank is provided with a discharge pipe.

[0007] Preferably, both the discharge pipe and the inlet pipe are equipped with flanges.

[0008] Preferably, telescopic cylinders are evenly installed on the top of one side of the primary and secondary enzymatic hydrolysis tanks, and the output end of the telescopic cylinders is provided with a drive arm connected to the top cover.

[0009] Preferably, the activated carbon deodorizing cylinder is provided with air inlets at both the bottom and top, and the interior of the activated carbon deodorizing cylinder is uniformly fitted with an activated carbon filter layer to facilitate the adsorption of odor gases released during enzymatic hydrolysis.

[0010] Preferably, the outer wall of the external temperature control heating jacket is provided with a heat-insulating rubber protective layer, which improves the heat insulation effect of the outer wall of the external temperature control heating jacket.

[0011] Preferably, a pH sensor is installed at one end of both the primary and secondary enzymatic hydrolysis tanks, and a pH adjustment port is provided on the top cover to facilitate intelligent monitoring of the pH status inside the primary and secondary enzymatic hydrolysis tanks in order to adjust the pH status.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] (1) The apparatus for producing crude heparin sodium from small intestinal mucosa is equipped with a secondary enzymatic hydrolysis tank, which optimizes the device's performance. The processed small intestinal mucosa slurry is added to the primary enzymatic hydrolysis tank through a corrosion-resistant pump on the feed pipe. External temperature-controlled heating jackets are fixed to the outer walls of both the primary and secondary enzymatic hydrolysis tanks. A water bath heating chamber with heating wires and temperature sensors installed inside the side wall of the external temperature-controlled heating jacket allows for temperature control and heating of both tanks. This is further enhanced by the timing function of the PLC controller. The control mechanism allows the material to react for 2 to 4 hours in a pre-controlled temperature environment of 45°C to 55°C inside the primary enzymatic hydrolysis tank. Then, it is automatically transferred to the secondary enzymatic hydrolysis tank via a corrosion-resistant pump on the transfer pipe. In this secondary tank, the material reacts for 1 to 2 hours in a pre-controlled temperature environment of 70°C to 90°C. This allows the device to achieve continuous two-stage temperature-controlled and timed reaction processing. The two-stage enzymatic hydrolysis technology results in better hydrolysis of the slurry, significantly improving the yield and purity of heparin sodium. Furthermore, the two-stage enzymatic hydrolysis greatly shortens the reaction time, resulting in better extraction.

[0014] (2) The device for producing crude heparin sodium from small intestinal mucosa is equipped with a servo motor, which optimizes the structure of the device. When the servo motor is started, it drives the stirring shaft to stir and mix the material inside the corresponding primary and secondary enzymatic hydrolysis tanks. This helps to improve the enzymatic hydrolysis effect and ensure that the small intestinal mucosal serous fluid and protease are fully and evenly contacted and reacted. At the same time, the rotation of the stirring shaft will drive the fan blades at the top to rotate, which helps to drive the gas flow in the top space inside the primary and secondary enzymatic hydrolysis tanks. This allows the gas generated during the enzymatic hydrolysis reaction to be continuously introduced and exported through the ventilation inlet on the activated carbon deodorizing cylinder. This allows the gas to be filtered out of odor molecules through the activated carbon filter layer inside the activated carbon deodorizing cylinder. Furthermore, the threaded connection structure between the activated carbon deodorizing cylinder and the top cover allows for the periodic replacement of the activated carbon deodorizing cylinder, which helps to avoid the problem of poor deodorization effect caused by the adsorption saturation of the activated carbon deodorizing cylinder and enhances its practicality. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0016] Figure 2 This is a frontal cross-sectional view of the present invention.

[0017] Figure 3 This is a partial cross-sectional view of the top cover of this utility model.

[0018] Figure 4 This is a schematic diagram of the rear view of the secondary enzymatic hydrolysis tank of this utility model;

[0019] Figure 5 This is a top view of a partial cross-sectional structure of the single-stage enzymatic hydrolysis vessel of this utility model.

[0020] In the diagram: 1. PLC controller; 2. Integrated frame; 3. Secondary enzymatic hydrolysis tank; 4. Primary enzymatic hydrolysis tank; 5. Servo motor; 6. Top cover; 7. pH sensor; 8. Transfer fitting; 9. Discharge fitting; 10. Corrosion-resistant pump; 11. Feed fitting; 12. Stirring shaft; 13. Activated carbon deodorizing cylinder; 14. Ventilation inlet; 15. Guide fan blade; 16. Telescopic cylinder; 17. Drive arm; 18. External temperature control heating jacket; 19. Heating wire; 20. Temperature sensor; 21. Water bath heating chamber. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0022] Please see Figure 1-5 An embodiment of this utility model provides an apparatus for producing crude heparin sodium from small intestinal mucosa, comprising an integrated frame 2, a PLC controller 1 installed on one side of the integrated frame 2, and a primary enzymatic hydrolysis tank 4 and a secondary enzymatic hydrolysis tank 3 respectively fixed at both ends inside the integrated frame 2.

[0023] A transfer pipe 8 is connected between the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3. A feed pipe 11 is provided at the top of the primary enzymatic hydrolysis tank 4. A corrosion-resistant pump 10 is installed on both the feed pipe 11 and the transfer pipe 8.

[0024] Both the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3 are fitted with a top cover 6. A servo motor 5 is installed on the top of the top cover 6. The output end of the servo motor 5 is connected to a stirring shaft 12. A guide fan blade 15 is provided on the top of the stirring shaft 12. An activated carbon deodorizing cylinder 13 that matches the guide fan blade 15 is evenly threaded to the bottom of the top cover 6.

[0025] The activated carbon deodorizing cylinder 13 is provided with ventilation inlets 14 at both the bottom and top. The activated carbon filter layer is uniformly packed inside the activated carbon deodorizing cylinder 13 to facilitate the adsorption of odor gases released during enzymatic hydrolysis.

[0026] During use, the servo motor 5 starts, driving the stirring shaft 12 to stir and mix the materials inside the corresponding primary enzymatic hydrolysis tank 4 and secondary enzymatic hydrolysis tank 3. This helps improve the enzymatic hydrolysis effect and ensures that the small intestinal mucosal serous fluid and protease fully and evenly contact and react. At the same time, the rotation of the stirring shaft 12 will drive the guide fan blade 15 at its upper position to rotate, which helps to drive the gas flow in the top space inside the primary enzymatic hydrolysis tank 4 and secondary enzymatic hydrolysis tank 3. This allows the gas generated during the enzymatic hydrolysis reaction to be continuously introduced and exported through the ventilation inlet 14 on the activated carbon deodorizing cylinder 13. This allows the gas to be filtered out of odor molecules through the activated carbon filter layer inside the activated carbon deodorizing cylinder 13. Furthermore, the threaded connection structure between the activated carbon deodorizing cylinder 13 and the top cover 6 allows for the periodic replacement of the activated carbon deodorizing cylinder 13, which helps to avoid the problem of poor deodorization effect caused by the adsorption saturation of the activated carbon deodorizing cylinder 13, thus enhancing its practicality.

[0027] Both the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3 are fitted with an external temperature control heating jacket 18. The interior of the side wall of the external temperature control heating jacket 18 is provided with a water bath heating chamber 21. The interior of the water bath heating chamber 21 is equipped with a heating wire 19 and a temperature sensor 20.

[0028] The bottom of the secondary enzymatic hydrolysis tank 3 is equipped with a discharge pipe 9;

[0029] Flanges are provided on both the discharge pipe fitting 9 and the inlet pipe fitting 11;

[0030] In use, the treated small intestinal mucosal slurry is added to the primary enzymatic hydrolysis tank 4 through the corrosion-resistant pump 10 on the feed pipe 11. External temperature-controlled heating jackets 18 are fixed to the outer walls of both the primary and secondary enzymatic hydrolysis tanks 4 and 3. A water bath heating chamber 21, equipped with heating wires 19 and temperature sensors 20, is located inside the side wall of the external temperature-controlled heating jacket 18. This allows for temperature control and heating of both tanks. Combined with the timer control of the PLC controller 1, the material reacts for 2 to 4 hours at a controlled temperature of 45°C to 55°C inside the primary enzymatic hydrolysis tank 4. Then, it is automatically transferred to the secondary enzymatic hydrolysis tank 3 via the corrosion-resistant pump 10 on the transfer pipe 8, where it reacts for 1 to 2 hours at a controlled temperature of 70°C to 90°C. This allows the device to achieve continuous two-stage temperature-controlled and timed reaction processing. The two-stage enzymatic hydrolysis technology improves the hydrolysis effect of the slurry, significantly increasing the yield and purity of heparin sodium. Furthermore, the two-stage enzymatic hydrolysis greatly shortens the reaction time, resulting in better extraction.

[0031] Telescopic cylinders 16 are evenly installed on the top of one side of the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3. The output end of the telescopic cylinder 16 is provided with a drive arm 17 connected to the top cover 6.

[0032] The outer wall of the external temperature control heating jacket 18 is provided with a heat-insulating rubber protective layer, which improves the heat insulation effect of the outer wall of the external temperature control heating jacket 18.

[0033] Both the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3 are equipped with a pH sensor 7 at one end, and the top cover 6 is provided with a pH adjustment port, so as to facilitate intelligent monitoring of the pH status inside the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3, and adjust the pH accordingly.

[0034] In this embodiment of the application, when in use: With an external power supply, the user can first start the secondary enzymatic hydrolysis tank 3. The drive arm 17 lifts the top cover 6 of both the primary and secondary enzymatic hydrolysis tanks 4 and 3. At this time, the user can pour a measured amount of protease into the primary enzymatic hydrolysis tank 4 and a measured amount of heparin-releasing enzyme into the secondary enzymatic hydrolysis tank 3. The treated small intestinal mucosal slurry can be added to the primary enzymatic hydrolysis tank 4 through the corrosion-resistant pump 10 on the feed pipe 11. Furthermore, a pH adjustment device can be connected to the pH adjustment port on the top cover 6, in conjunction with the corresponding pH sensors installed on the primary and secondary enzymatic hydrolysis tanks 4 and 3. The monitoring and feedback function of 7 adjusts the pH value. Furthermore, by fixing external temperature-controlled heating jackets 18 to the outer walls of both the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3, and cooperating with the water bath heating chamber 21 with heating wires 19 and temperature sensors 20 installed inside the side wall of the external temperature-controlled heating jackets 18, the temperature of the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3 can be controlled and heated. In addition, with the timer control function of the PLC controller 1, the material can be reacted for 2 to 4 hours in the temperature environment of 45°C to 55°C inside the primary enzymatic hydrolysis tank 4, and then automatically transferred to the secondary enzymatic hydrolysis tank through the corrosion-resistant pump 10 on the transfer pipe fitting 8. Inside tank 3, the reaction proceeds for 1 to 2 hours at a controlled temperature of 70°C to 90°C, enabling continuous two-stage temperature-controlled and timed reaction processing. This dual enzymatic hydrolysis technique significantly improves the hydrolysis effect of the slurry, increasing the yield and purity of heparin sodium. Furthermore, the two hydrolysis processes greatly shorten the reaction time, resulting in better extraction. Additionally, the servo motor 5 activates, driving the stirring shaft 12 to stir and mix the materials inside the corresponding primary and secondary hydrolysis tanks 4 and 3. This enhances the hydrolysis effect, ensuring thorough and uniform contact between the small intestinal mucosal slurry and the protease. Simultaneously, the rotation of the stirring shaft 12... The rotating fan blades 15 at the top of the activated carbon deodorizing cylinder 13 facilitate the flow of gas in the top space inside the primary enzymatic hydrolysis tank 4 and the secondary enzymatic hydrolysis tank 3. This allows the gas generated during the enzymatic hydrolysis reaction to be continuously introduced and exported through the ventilation inlet 14 on the activated carbon deodorizing cylinder 13. This allows the gas to be filtered out of odor molecules by the activated carbon filter layer inside the activated carbon deodorizing cylinder 13. Furthermore, the threaded connection between the activated carbon deodorizing cylinder 13 and the top cover 6 allows for the periodic replacement of the activated carbon deodorizing cylinder 13, which helps to avoid the problem of poor deodorization effect caused by the activated carbon deodorizing cylinder 13 becoming saturated, thus enhancing its practicality.

Claims

1. An apparatus for producing crude heparin sodium from the small intestinal mucosa, characterized in that, The system includes an integrated frame (2), on one side of which a PLC controller (1) is installed. A primary enzymatic hydrolysis tank (4) and a secondary enzymatic hydrolysis tank (3) are fixed at both ends inside the integrated frame (2). A transfer pipe (8) connects the primary and secondary enzymatic hydrolysis tanks (3). A feed pipe (11) is installed at the top of the primary enzymatic hydrolysis tank (4). A corrosion-resistant pump (10) is installed on both the feed pipe (11) and the transfer pipe (8). A top cover (6) is fitted onto the top of both the primary and secondary enzymatic hydrolysis tanks (4 and 3). A servo motor (5) is installed, and the output end of the servo motor (5) is connected to a stirring shaft (12). A guide fan blade (15) is provided on the top of the stirring shaft (12). An activated carbon deodorizing cylinder (13) matching the guide fan blade (15) is evenly threaded to the bottom of the top cover (6). Both the primary enzymatic hydrolysis tank (4) and the secondary enzymatic hydrolysis tank (3) are fitted with an external temperature control heating sleeve (18). A water bath heating chamber (21) is provided inside the side wall of the external temperature control heating sleeve (18). An electric heating wire (19) and a temperature sensor (20) are installed inside the water bath heating chamber (21).

2. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 1, characterized in that: The bottom end of the secondary enzymatic hydrolysis tank (3) is provided with a discharge pipe (9).

3. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 2, characterized in that: Both the discharge pipe fitting (9) and the inlet pipe fitting (11) are equipped with flanges.

4. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 1, characterized in that: Telescopic cylinders (16) are evenly installed on the top of one side of the primary enzymatic hydrolysis tank (4) and the secondary enzymatic hydrolysis tank (3). The output end of the telescopic cylinder (16) is provided with a drive arm (17) connected to the top cover (6).

5. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 1, characterized in that: The activated carbon deodorizing cylinder (13) is provided with ventilation inlets (14) at both the bottom and top, and the activated carbon filter layer is uniformly packed inside the activated carbon deodorizing cylinder (13).

6. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 1, characterized in that: The outer wall of the external temperature control heating jacket (18) is provided with a heat-insulating rubber protective layer.

7. The apparatus for preparing crude heparin sodium from small intestinal mucosa according to claim 1, characterized in that: A pH sensor (7) is installed at one end of both the primary enzymatic hydrolysis tank (4) and the secondary enzymatic hydrolysis tank (3), and a pH adjustment port is provided on the top cover (6).

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