Rapid liquid changing cell culture device

Abstract

The utility model discloses a cell culture device capable of rapidly changing liquid, belongs to the technical field related to cell culture, and aims to solve the problems that in the prior art, culture bottles need to be manually shaken by an operator during liquid change, so that cells are cleaned, but when the culture bottles needing to be cleaned are too many, the operator cannot completely shake the culture bottles, and the operation is inconvenient. The cell culture device comprises an ultra-clean workbench, a motor is installed at the lower end of the ultra-clean workbench, an output shaft of the motor is fixedly connected with a cam, the outer wall of the cam is attached to a baffle, and the baffle is fixedly connected with the ultra-clean workbench. The upper end of the baffle is fixedly connected with a movable plate, and the outer wall of the movable plate is slidably connected with an opening in the surface of the clean bench.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, and more specifically, relates to a rapid medium-change cell culture device. Background Technology

[0002] Cell culture is a technique that simulates the in vivo environment outside the body, allowing cells to survive, grow, multiply, and maintain their main structures and functions. During cell culture, the medium generally needs to be changed every 2-3 days.

[0003] Current cell culture devices require operators to manually shake the culture flasks during medium changes to clean the cells. However, when there are too many culture flasks to clean, the operator cannot shake them all, which increases the time required for medium changes and reduces the efficiency of the cell culture device. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a rapid medium-change cell culture device. This solves the problem that existing technologies require operators to manually shake the culture flasks during medium changes to clean the cells. However, when there are too many culture flasks to clean, the operator cannot shake them all, which increases the time required for medium changes and reduces the efficiency of the cell culture device.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a rapid medium-changing cell culture device, including an ultra-clean workbench, a motor installed at the lower end of the ultra-clean workbench, the output shaft of the motor being fixedly connected to a cam, the outer wall of the cam being in contact with a baffle, the upper end of the baffle being fixedly connected to a movable plate, and the outer wall of the movable plate being slidably connected to an opening on the surface of the ultra-clean workbench.

[0006] As a preferred embodiment of this utility model, the upper end of the movable plate is fixedly connected to the fixed block, and the inner wall of the fixed block is threadedly connected to the threaded rod through a through hole.

[0007] As a preferred embodiment of this utility model, the upper surface of the movable plate is fixedly connected to the second clamping plate, and a rubber pad is installed on the surface of the second clamping plate.

[0008] As a preferred embodiment of this utility model, one end of the threaded rod is fixedly connected to the knob, and the other end of the threaded rod is rotatably connected to the first clamping plate through a bearing. A rubber pad is installed on the surface of the first clamping plate.

[0009] As a preferred embodiment of this utility model, protective plates are installed on both sides of the ultra-clean workbench.

[0010] As a preferred embodiment of this utility model, the two sides of the movable plate are fixedly connected to a plurality of round rods, the outer walls of the plurality of round rods are slidably connected to corresponding sleeve rods, the ends of the plurality of round rods are fixedly connected to corresponding springs, the other ends of the plurality of springs are fixedly connected to corresponding sleeve rods, and the outer walls of the plurality of sleeve rods are fixedly connected to the clean bench.

[0011] This invention provides a rapid medium-change cell culture device, which has the following beneficial effects:

[0012] 1. This new invention utilizes a motor, cam, baffle, and movable plate. When it is necessary to shake the culture flask, the motor drives the cam to rotate, the cam drives the baffle to move, the baffle drives the movable plate above to move, and the movable plate drives the culture flask fixed above to move, thereby shaking the PBS buffer in the culture flask to wash the cells. In this way, multiple culture flasks can be shaken synchronously, eliminating the need for operators to shake them individually. This reduces the time for changing the medium in the cell culture device, thereby improving the working efficiency of the cell culture device.

[0013] 2. Using a fixing block, threaded rod, knob, second clamping plate, first clamping plate, round rod, sleeve rod, and spring, when the culture flask needs to be fixed, the operator turns the knob. The knob drives the threaded rod to rotate, which in turn moves the first clamping plate towards the second clamping plate. After the rubber pads on the surfaces of the first and second clamping plates are firmly against the culture flask, the operator stops turning the knob. When the movable plate moves, the round rod slides inside the sleeve rod. At this time, the round rod on one side of the movable plate compresses the spring, while the spring at the other end extends. This cushions the movement of the movable plate. In this way, the culture flask can be prevented from falling when shaken, thereby improving the working efficiency of the cell culture device. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0015] Figure 2 for Figure 1 A diagram showing the view from below;

[0016] Figure 3 for Figure 2 A left-view diagram;

[0017] Figure 4 for Figure 3 A cross-sectional view of the central rod, sleeve, and spring.

[0018] In the diagram: 1. Clean bench; 2. Protective plate; 3. Motor; 4. Cam; 5. Baffle; 6. Movable plate; 7. Round rod; 8. Sleeve rod; 9. Fixing block; 10. Threaded rod; 11. Knob; 12. First clamping plate; 13. Second clamping plate; 14. Spring. Detailed Implementation

[0019] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0020] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0022] Please see Figures 1 to 4 This invention provides a rapid medium-change cell culture device solution, comprising a clean bench 1, with a motor 3 installed at the lower end of the clean bench 1. The model of the motor 3 is selected according to actual needs, meeting the operational requirements. The output shaft of the motor 3 is fixedly connected to a cam 4, and the motor 3 drives the cam 4 to rotate. The outer wall of the cam 4 is in contact with a baffle 5, and the rotation of the cam 4 pushes the baffle 5 to move. The upper end of the baffle 5 is fixedly connected to a movable plate 6, and the baffle 5 drives the movable plate 6 above to move. The outer wall of the movable plate 6 is slidably connected to an opening on the surface of the clean bench 1, allowing the movable plate 6 to slide on the clean bench 1. When it is necessary to shake the culture flasks, the motor 3 drives the cam 4 to rotate, the cam 4 drives the baffle 5 to move, the baffle 5 drives the movable plate 6 above to move, and the movable plate 6 drives the fixed culture flask above to move, thereby shaking the PBS buffer in the culture flasks to wash the cells. This method allows multiple culture flasks to be shaken synchronously, eliminating the need for individual shaking by the operator, thus reducing the medium-change time of the cell culture device and improving its working efficiency.

[0023] The upper end of the movable plate 6 is fixedly connected to the fixed block 9. The inner wall of the fixed block 9 is threadedly connected to the threaded rod 10 through a through hole. The threaded rod 10 rotates in the fixed block 9. Since the fixed block 9 has threads inside, the first clamping plate 12 can move when the threaded rod 10 rotates. Since the lower surface of the first clamping plate 12 is in close contact with the movable plate 6, the first clamping plate 12 can move towards the side closer to the second clamping plate 13.

[0024] The upper surface of the movable plate 6 is fixedly connected to the second clamping plate 13. A rubber pad is installed on the surface of the second clamping plate 13, which can prevent the second clamping plate 13 from damaging the culture bottle.

[0025] One end of the threaded rod 10 is fixedly connected to the knob 11, which allows the operator to rotate the threaded rod 10. The other end of the threaded rod 10 is rotatably connected to the first clamping plate 12 via a bearing. The threaded rod 10 rotates on the first clamping plate 12, so the first clamping plate 12 will not rotate with the threaded rod 10. A rubber pad is installed on the surface of the first clamping plate 12, which can prevent the first clamping plate 12 from damaging the culture bottle.

[0026] Among them, protective plates 2 are installed on both sides of the ultra-clean workbench 1.

[0027] The movable plate 6 is fixedly connected to multiple round rods 7 on both sides. The round rods 7 are fixed on both sides of the movable plate 6. The outer walls of the multiple round rods 7 are slidably connected to the corresponding sleeve rods 8. The round rods 7 slide inside the sleeve rods 8. The ends of the multiple round rods 7 are fixedly connected to the corresponding springs 14. When the round rods 7 move, they can extend or compress the springs 14. The springs 14 can slow down the movement speed of the movable plate 6. The other ends of the multiple springs 14 are fixedly connected to the corresponding sleeve rods 8. The springs 14 are fixed inside the sleeve rods 8. The outer walls of the multiple sleeve rods 8 are fixedly connected to the clean bench 1. The sleeve rods 8 are fixed on the clean bench 1.

[0028] The specific usage and function of this embodiment are as follows:

[0029] In use, the necessary equipment for changing the culture medium is first placed on the clean bench 1. Then, the culture medium is aspirated from the culture flask using a pipette. Next, PBS buffer is poured into the culture flask, and the flask is placed on the movable plate 6, positioned between the first clamp 12 and the second clamp 13. The operator then rotates knob 11, which rotates the threaded rod 10. The threaded rod 10 moves the first clamp 12 towards the second clamp 13. Once the rubber pads on both clamps 12 and 13 are firmly against the culture flask, the operator stops rotating knob 11. Then, the external power supply to motor 3 is connected, and motor 3 is started. Motor 3 rotates cam 4, which pushes baffle 5 to move. Baffle 5 then moves the movable plate 6. 6 moves the culture flask above, causing the PBS buffer inside the flask to shake and clean the cell surface. When the movable plate 6 moves, the round rod 7 slides inside the sleeve rod 8. At this time, the round rod 7 on one side of the movable plate 6 presses the spring 14, while the spring 14 on the other end extends, which buffers the movement of the movable plate 6 and prevents the PBS buffer from spilling. After the shaking is complete, the motor 3 stops working. The operator uses a pipette to draw out the PBS buffer and then sends new PBS buffer into the culture flask. After cleaning the cells several times in the above manner, the operator rotates the knob 11 in the opposite direction. Through the above transmission method, the first clamp 12 is moved away from the culture flask. Then the operator stops rotating the knob 11, removes the culture flask, and proceeds to the next step of changing the medium.

[0030] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A rapid medium-change cell culture device, characterized in that: The device includes a clean bench, with a motor installed at the lower end of the clean bench. The output shaft of the motor is fixedly connected to a cam. The outer wall of the cam is in contact with a baffle. The upper end of the baffle is fixedly connected to a movable plate. The outer wall of the movable plate is slidably connected to an opening on the surface of the clean bench.

2. The rapid medium-change cell culture device according to claim 1, characterized in that: The upper end of the movable plate is fixedly connected to the fixed block, and the inner wall of the fixed block is threadedly connected to the threaded rod through a through hole.

3. The rapid medium-change cell culture device according to claim 1, characterized in that: The upper surface of the movable plate is fixedly connected to the second clamping plate, and a rubber pad is installed on the surface of the second clamping plate.

4. The rapid medium-change cell culture device according to claim 2, characterized in that: One end of the threaded rod is fixedly connected to the knob, and the other end of the threaded rod is rotatably connected to the first clamping plate through a bearing. A rubber pad is installed on the surface of the first clamping plate.

5. The rapid medium-change cell culture device according to claim 1, characterized in that: The clean bench is equipped with protective panels on both sides.

6. The rapid medium-change cell culture device according to claim 1, characterized in that: The movable plate is fixedly connected to multiple round rods on both sides. The outer walls of the multiple round rods are slidably connected to corresponding sleeve rods. The ends of the multiple round rods are fixedly connected to corresponding springs. The other ends of the multiple springs are fixedly connected to corresponding sleeve rods. The outer walls of the multiple sleeve rods are fixedly connected to the clean bench.

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