Separation device for sphingomonas

Abstract

The utility model discloses a sphingomonas separating device which comprises a base body and a sealing cover matched with the base body, the structure of the sealing cover is improved in the scheme, the sealing cover is composed of a first cover body and a second cover body, the second cover body can rotate relative to the first cover body, and then a second feeding hole corresponds to a first feeding hole. Culture solution can be input into the base body through the second feeding hole and the first feeding hole according to requirements, the size of the second feeding hole and the size of the supporting column are limited, after the culture solution is input, the second cover body is rotated to enable the second feeding hole to correspond to the first feeding hole, and the elastic block abuts against the supporting column so that the second feeding hole can be sealed; corresponding culture solutions can be conveniently input into different areas according to requirements during streak separation, and the influence of external factors in the culture process can be reduced.

Description

Technical Field

[0001] This utility model relates to the field of separation device technology, specifically to a separation device for Sphingosine Monoclonal bacteria. Background Technology

[0002] Sphingosine monocytogenes exhibits good degradation effects on aromatic compounds such as phenanthrene and bromoacetic acid in soil, making it a hot research area for the remediation of polycyclic aromatic hydrocarbon-contaminated soils. During the screening and cultivation process, Sphingosine monocytogenes is generally isolated by streak plating on petri dishes. Some strains require periodic or irregular addition of culture medium during cultivation, depending on demand. Conventional petri dishes require the cap to be removed before adding these substances, an operation that is susceptible to external influences such as the presence of bacterial agents within the petri dish, and therefore needs improvement. Utility Model Content

[0003] To address at least one of the aforementioned technical deficiencies, this utility model provides the following technical solution:

[0004] This application discloses an isolation device for Sphingomonas sphingosine mononitrate, comprising a substrate and a matching cap. The cap includes a first cap and a second cap. The top surface of the first cap has a concentric annular groove, and the sides of the second cap are slidably connected to the annular groove. The top surface of the first cap has a plurality of first feeding holes spaced apart circumferentially, and the partition between adjacent first feeding holes forms a support column. The top surface of the second cap has a plurality of second feeding holes spaced apart circumferentially, and the bottom surface of the second cap between adjacent second feeding holes has an elastic block. The width of the second feeding hole is smaller than the width of the support column. When the second cap is rotated so that the second feeding hole is directly above the support column, the elastic blocks on both sides of the second feeding hole abut against the support column.

[0005] This design improves the structure of the cap. The first cap and the second cap together form the cap. The second cap can rotate relative to the first cap, thereby aligning the second feeding hole with the first feeding hole. Culture medium can be input into the substrate through the second feeding hole and the first feeding hole as needed. The dimensions of the second feeding hole and the support are limited. After the culture medium is input, the second cap is rotated to align the second feeding hole with the first feeding hole. An elastic block abuts against the support to seal the second feeding hole. This facilitates the input of the corresponding culture medium to different areas as needed during scribing and separation, and reduces the influence of external factors.

[0006] Furthermore, annular protrusions are concentrically provided on the top surface of the first cover, and annular grooves are formed on the top surface of the protrusions to facilitate docking with the first cover.

[0007] Furthermore, four first feeding holes are formed at circumferential intervals on the top surface of the first cover, and four second feeding holes are formed at circumferential intervals on the top surface of the second cover.

[0008] Furthermore, a protruding post is provided at the center of the top surface of the first cover, and a rotating shaft is provided at the bottom surface of the second cover. The rotating shaft extends into the shaft hole formed on the top surface of the protruding post for rotatable connection, which facilitates the rotatable connection between the first cover and the second cover.

[0009] Furthermore, the first cover is in the shape of a groove, and an elastic ring 1 is concentrically and outwardly provided on the inner wall of the groove of the first cover, and an elastic ring 2 is concentrically and outwardly provided on the outer wall of the groove of the base, which helps to improve the sealing performance and reduce the influence of external factors.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0011] 1. This utility model improves the structure of the cap, with the first cap and the second cap forming the cap, which can introduce the corresponding culture medium into different areas of the substrate, making it convenient to operate the streak isolation and culture of Sphingosine monocytogenes. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the cross-sectional structure of the separation device in Example 1;

[0014] Figure 2 This is a schematic diagram of the second cover structure viewed from below;

[0015] Figure 3 This is a top view of the structure of the first cover.

[0016] The attached figures are labeled as follows:

[0017] 1. Base; 2. Cover; 3. Elastic ring one; 4. Elastic ring two; 21. First cover; 22. Protruding rib; 23. Second cover; 24. Second feeding hole; 25. Elastic block; 26. Rotating shaft; 27. Groove wall; 28. First feeding hole; 29. ​​Support column; 30. Ring groove; 31. Shaft hole. Detailed Implementation

[0018] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0019] Example 1

[0020] like Figure 1 , Figure 2 , Figure 3As shown, the isolation device for Sphingomonas in this example includes a substrate and a matching cap. The substrate is a common trough shape. The cap includes a first cap and a second cap. The top surface of the first cap has concentric annular protrusions, and the top surface of the protrusions has concentric annular grooves. The second cap is in the shape of a circular groove, and its groove wall is inserted into the annular groove for sliding fit. When in use, the second cap is rotated relative to the first cap.

[0021] Multiple first feeding holes, such as four, are formed at circumferential intervals on the top surface of the first cover within the annular opening formed by the protruding ribs. The space between adjacent first feeding holes forms a support column. Multiple second feeding holes, such as four, are formed at circumferential intervals on the top surface of the second cover corresponding to the annular opening. An elastic block, such as made of rubber, is fixed to the bottom surface of the second cover between adjacent second feeding holes. The elastic block is fixed to the bottom surface of the second cover by means of adhesive or other methods.

[0022] The width of the second feeding hole is smaller than the width of the support column. When the second cover is rotated so that the second feeding hole is directly above the support column and the elastic blocks on both sides of the second feeding hole simultaneously abut against the support column, the elastic blocks on both sides cooperate with the support column to form a seal on the second feeding hole. In this state, the cover forms a good sealing effect on the substrate.

[0023] An elastic layer, such as a rubber layer, can be wrapped around the outer peripheral wall of the groove of the first cover to help improve the sealing with the annular groove. A handle can also be installed at the center of the top surface of the second cover to make it easier to rotate.

[0024] In this example, a protruding post is formed at the center of the top surface of the first cover, and a shaft hole is formed longitudinally at the top surface of the protruding post. A rotating shaft is formed outward at the center of the bottom surface of the second cover, and the rotating shaft extends into the shaft hole formed at the top surface of the protruding post to form a rotating connection, thereby improving the stability of the rotating connection between the first cover and the second cover.

[0025] An elastic ring one, made of rubber, can be concentrically fixed to the groove wall of the first cover, with a portion protruding outward from the groove wall. An elastic ring two, also concentrically fixed to the groove peripheral wall of the base, also has a portion protruding outward from the groove peripheral wall of the base. Figure 1 As shown, when the first cover is placed on the substrate, the first elastic ring is compressed and deformed to pass over the second elastic ring, which helps to improve the sealing performance and reduce the influence of external factors.

[0026] In use, if the second cover rotates relative to the first cover, the second feeding hole will correspond to the first feeding hole. Rotating the second cover will adjust the second feeding hole to face the corresponding area in the substrate, and then the culture medium can be input into the substrate through the second feeding hole and the first feeding hole.

[0027] The above are merely preferred embodiments of this utility model. The protection scope of this utility model is not limited to the above embodiments. All technical solutions falling within the scope of this utility model's concept are within its protection scope. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of this utility model should also be considered within its protection scope.

Claims

1. An apparatus for isolating Sphingosine monocytogenes, comprising a substrate and a matching cap, characterized in that, The cover includes a first cover and a second cover. The top surface of the first cover is concentrically provided with an annular groove and the side of the second cover is slidably connected to the annular groove. The top surface of the first cover is provided with a plurality of first feeding holes spaced apart circumferentially, and the partition between adjacent first feeding holes forms a support column. The top surface of the second cover is provided with a plurality of second feeding holes spaced apart circumferentially, and the bottom surface of the second cover between adjacent second feeding holes is provided with an elastic block. The width of the second feeding hole is less than the width of the support column. When the second cover is rotated so that the second feeding hole is directly above the support column, the elastic blocks on both sides of the second feeding hole abut against the support column.

2. The apparatus for isolating Sphingomonas as described in claim 1, characterized in that: The top surface of the first cover is provided with concentric annular protrusions, and the top surface of the protrusions is formed with an annular groove.

3. The apparatus for isolating Sphingomonas as described in claim 1, characterized in that: The top surface of the first cover has four first feeding holes spaced apart circumferentially, and the top surface of the second cover has four second feeding holes spaced apart circumferentially.

4. The apparatus for isolating Sphingomonas as described in claim 1, characterized in that: A protruding post is provided at the center of the top surface of the first cover, and a rotating shaft is provided at the bottom surface of the second cover. The rotating shaft extends into a shaft hole formed at the top surface of the protruding post for rotatable connection.

5. The apparatus for isolating Sphingomonas as described in claim 1, characterized in that: The first cover is in the shape of a groove, and an elastic ring 1 is concentrically and outwardly provided on the inner wall of the groove of the first cover, and an elastic ring 2 is concentrically and outwardly provided on the outer wall of the groove of the base.

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