Chemiluminescence immunoassay analyzer

Abstract

The utility model belongs to the technical field of detection instruments, and discloses a chemiluminescence immunoassay analyzer. The chemiluminescence immunity analyzer comprises a rack, and a sample adding mechanism, a reagent adding mechanism, an incubation mechanism, a magnetic separation cleaning mechanism, a reagent storage mechanism, a sample injection mechanism, a photoelectric detection mechanism, a uniform mixing mechanism, a reaction cup arrangement mechanism and a cup grabbing hand mechanism which are arranged on the rack. The rack of the chemiluminescence immunoassay analyzer is designed to be rectangular, all mechanisms are arranged on the rack in a planar one-layer integrated manner, the layout scheme is reasonable and efficient, and the integration is high.

Description

Technical Field

[0001] This application pertains to the field of testing instrument technology, and in particular relates to a chemiluminescence immunoassay analyzer. Background Technology

[0002] Chemiluminescence immunoassay has advantages such as high sensitivity, high specificity, wide linear range, and high degree of automation, and has become one of the main methods of clinical diagnosis. Currently, commercially available benchtop fully automated chemiluminescence immunoassay analyzers have a dispersed layout of modules, resulting in a large size; furthermore, they use only one needle for sample and reagent addition, which easily leads to cross-contamination of samples and reagents; and the shared system for sample and reagent addition results in low sample addition accuracy, thus affecting the accuracy of test results. Summary of the Invention

[0003] Purpose of the utility model: In view of the above-mentioned problems existing in the prior art, this application provides a highly integrated, efficient chemiluminescent immunoassay analyzer that separates sample addition and reagent addition, thereby reducing cross-contamination.

[0004] Technical Solution: This application discloses a chemiluminescence immunoassay analyzer, including a frame, and a sample loading mechanism, a reagent loading mechanism, an incubation mechanism, a magnetic separation and cleaning mechanism, a reagent storage mechanism, a sample injection mechanism, a photoelectric detection mechanism, a mixing mechanism, a reaction cup handling mechanism, and a cup gripper mechanism disposed on the frame. The reagent storage mechanism is arranged on one side of the analyzer, and the sample injection mechanism is located near the reagent storage mechanism. The incubation mechanism and the magnetic separation and cleaning mechanism are sequentially arranged on the other side of the analyzer from the reagent storage mechanism. The reaction cup handling mechanism is located near the magnetic separation and cleaning mechanism. The sample loading mechanism is disposed between the sample injection mechanism and the incubation mechanism for adding samples to the incubation mechanism. The reagent loading mechanism is disposed between the reagent storage mechanism and the incubation mechanism for adding reagents to the incubation mechanism. The cup gripper mechanism is disposed between the magnetic separation and cleaning mechanism and the incubation mechanism. The photoelectric detection mechanism and the mixing mechanism are both disposed between the reaction cup handling mechanism and the incubation mechanism and are within the travel range of the cup gripper mechanism, facilitating the cup gripper mechanism's handling of reaction cups between the magnetic separation and cleaning mechanism, the incubation mechanism, and the photoelectric detection mechanism.

[0005] The sample addition mechanism is used for sample addition, and the reagent addition mechanism is used for reagent addition. Both the sample addition mechanism and the reagent addition mechanism are rotary sampling / reagent collection needle structures.

[0006] As a technical solution, the chemiluminescence immunoassay analyzer is an integrated planar single-layer distribution. That is, all mechanisms, including the sample addition mechanism, reagent addition mechanism, incubation mechanism, magnetic separation and cleaning mechanism, reagent storage mechanism, sample injection mechanism, photoelectric detection mechanism, mixing mechanism, reaction cup handling mechanism, and cup gripper mechanism, are all set in the same plane of the frame, resulting in high integration.

[0007] As a technical solution, the sample injection mechanism includes an X-component for injection and a Y-component for mounting. The X-component includes a mounting frame, a sample tray, and an injection drive. The sample tray is mounted on the mounting frame via a slide rail and reciprocates in the X direction under the drive of the injection drive. The sample tray has parallel sample rack placement slots that extend from front to back. The mounting frame has an inlet, and the Y-component for mounting is installed at the position corresponding to the inlet. The Y-component for mounting moves the sample rack corresponding to the inlet to the injection position along the Y direction.

[0008] As a technical solution, the Y-shaped feed rack assembly includes a second mounting frame, a hook rod, and a feed rack drive component. The feed rack drive component is mounted on the second mounting frame, and the hook rod moves back and forth along the Y direction under the drive of the feed rack drive component, hooking the sample rack located at the feed rack inlet to the sample feeding position or sending it back to the sample rack placement slot.

[0009] As a technical solution, the sample holder is provided with a straight groove at the position corresponding to the stroke of the hook rod, which facilitates the insertion and hooking of the hook rod. Specifically, the hook rod can be a rod extending vertically, and the straight groove extends along the X direction. When the hook rod reaches the working position, it is located below the inlet. The sample holder moves to the inlet along with the sample tray, and the straight groove of the sample holder matches the hook rod as it extends. The inlet drive drives the hook rod to move along the Y direction to the sampling position for sampling. After sampling, the inlet drive drives the hook rod to move in the opposite direction along the Y direction back to the initial position, and the sample tray moves to the next sample holder under the action of the sample feeding drive.

[0010] As a technical solution, the mounting frame includes a base plate and a rear side plate. The sample tray is slidably mounted on the base plate via a slide rail, and a buffer plate is provided on the rear side plate. With the buffer plate installed on the rear side plate, if the sample tray slides too far backward after being placed in the sample tray placement slot, it will gradually return to its stable position under the action of the buffer plate after contacting it.

[0011] As a technical solution, the mixing mechanism includes a mixing drive and a mixing assembly. The mixing assembly includes a rotating seat with a cup holder on it. The rotating seat is eccentrically positioned relative to the drive shaft of the mixing drive.

[0012] As a technical solution, the mixing mechanism further includes a rotation limiting component, which includes a bearing seat. The bearing seat is provided with a vertical mounting hole and a horizontal mounting hole. The bearing seat is rotatably mounted in the vertical direction through the vertical mounting hole. A limiting shaft is slidably provided in the horizontal mounting hole, and the other end of the limiting shaft is connected to a rotating seat.

[0013] As one technical solution, the eccentricity d between the rotating seat and the drive shaft of the mixing drive is 1mm to 4mm.

[0014] The parts, structures, and components not described in this application, as well as their positional and connection relationships, can all be implemented using existing technologies.

[0015] Beneficial effects: Compared with the prior art, the chemiluminescence immunoassay analyzer of this application has all its components arranged in a single layer on the rack in a planar manner, which is reasonable, efficient and highly integrated; the design of separate sample addition and reagent addition further reduces cross-contamination during the detection process. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of a chemiluminescence immunoassay analyzer.

[0017] Figure 2 This is a schematic diagram of the sample injection mechanism.

[0018] Figure 3 This is a schematic diagram of the Y-assembly structure.

[0019] Figure 4 This is a schematic diagram of the hook rod hooking the sample rack;

[0020] Figure 5 This is a schematic diagram of the mixing mechanism.

[0021] Figure 6 This is a cross-sectional schematic diagram of the mixing mechanism;

[0022] Figure 7 This is an exploded view of the rotating limit component structure;

[0023] Figure 8 This is a schematic diagram of the sample dispensing mechanism / reagent dispensing mechanism;

[0024] Figure 9 This is a schematic diagram of the incubation facility structure;

[0025] Figure 10 This is a schematic diagram of the magnetic separation cleaning mechanism;

[0026] Figure 11 This is a schematic diagram of the magnetic separation cleaning mechanism from another perspective;

[0027] Figure 12 This is a schematic diagram of the reagent storage mechanism;

[0028] Figure 13 This is a schematic diagram of the photoelectric detection mechanism.

[0029] Figure 14 This is a schematic diagram of the reaction cup sorting mechanism;

[0030] Figure 15 This is a schematic diagram of the cup-grabbing mechanism.

[0031] In the diagram, the components are: sample addition mechanism 1, reagent addition mechanism 2, incubation mechanism 3, magnetic separation and cleaning mechanism 4, reagent storage mechanism 5, sample injection mechanism 6, photoelectric detection mechanism 7, mixing mechanism 8, reaction cup sorting mechanism 9, and cup gripping mechanism 10; mounting frame 1 61, sample tray 62, injection drive component 63, sample rack placement slot 64, rack inlet 65, mounting frame 2 66, hook rod 67, rack inlet drive component 68, straight groove 69, base plate 611, rear side plate 612, buffer plate 613, mixing drive component 81, mixing assembly 82, rotation limiting assembly 83, rotating seat 821, cup holder 822, drive shaft 811, bearing seat 831, vertical mounting hole 8311, horizontal mounting hole 8312, and limiting shaft 832. Detailed Implementation

[0032] The present application will now be described in detail with reference to specific embodiments.

[0033] Example 1

[0034] like Figure 1 The chemiluminescence immunoassay analyzer shown includes a frame, and on the frame are the following components: sample loading mechanism 1, reagent loading mechanism 2, incubation mechanism 3, magnetic separation and cleaning mechanism 4, reagent storage mechanism 5, sample injection mechanism 6, photoelectric detection mechanism 7, mixing mechanism 8, reaction cup handling mechanism 9, and cup gripper mechanism 10. The frame of the chemiluminescence immunoassay analyzer is rectangular or square, and all mechanisms are integrated and arranged on the frame in a planar, single-layer distribution. That is, sample loading mechanism 1, reagent loading mechanism 2, incubation mechanism 3, magnetic separation and cleaning mechanism 4, reagent storage mechanism 5, sample injection mechanism 6, photoelectric detection mechanism 7, mixing mechanism 8, reaction cup handling mechanism 9, and cup gripper mechanism 10 are all located on the same plane of the frame, demonstrating high integration.

[0035] The reagent storage mechanism 5 is located near the analyzer, and the sample injection mechanism 6 is located near the end of the reagent storage mechanism 5. An incubation mechanism 3 and a magnetic separation and cleaning mechanism 4 are sequentially arranged on the opposite side of the analyzer from the reagent storage mechanism 5. A reaction cup handling mechanism 9 is located near the end of the magnetic separation and cleaning mechanism 4. A sample addition mechanism 1 is located between the sample injection mechanism 6 and the incubation mechanism 3 to add samples to the incubation mechanism 3. A reagent addition mechanism 2 is located between the reagent storage mechanism 5 and the incubation mechanism 3 to add reagents to the incubation mechanism 3. A cup-gripping mechanism 10 is located between the magnetic separation and cleaning mechanism 4 and the incubation mechanism 3. A photoelectric detection mechanism 7 and a mixing mechanism 8 are both located between the reaction cup handling mechanism 9 and the incubation mechanism 3, and are within the stroke range of the cup-gripping mechanism 10, facilitating the cup-gripping mechanism 10 in handling the reaction cups between the magnetic separation and cleaning mechanism 4, the incubation mechanism 3, and the photoelectric detection mechanism 7.

[0036] In this embodiment of the chemiluminescence immunoassay analyzer, the incubation mechanism 3 is equipped with a sample loading station. The cup-grabbing mechanism 10 can pick up the reaction cups that are taken out of the reaction cup sorting mechanism 9 as a whole and transfer them between the sample loading station, the mixing mechanism 8, the magnetic separation and cleaning mechanism 4, and the photoelectric detection mechanism 7. The sample loading mechanism 1 is used to pick up the sample from the sample injection mechanism 6 and add it into the reaction cup. The reagent loading mechanism 2 is used to pick up the reagent from the reagent storage mechanism 5 and add it into the reaction cup. The mixing mechanism 8 is used to mix the sample and reagent in the reaction cup. The magnetic separation and cleaning mechanism 4 is used to clean the reaction cup of interfering substances other than magnetic particles and target analytes. The photoelectric detection mechanism 7 is used to detect the luminescence signal of the substances in the reaction cup.

[0037] Combination Figure 2 The sample injection mechanism 6 includes an X-component for injection and a Y-component for mounting. The X-component includes a mounting frame 61, a sample tray 62, and an injection drive 63. The sample tray 62 is mounted on the mounting frame 61 via a slide rail and reciprocates in the X direction under the drive of the injection drive 63. The sample tray 62 has sample rack placement slots 64 that run through it. The mounting frame 61 has an inlet 65, and the Y-component for mounting is installed at the position corresponding to the inlet 65. The Y-component for mounting moves the sample rack located in the sample placement slot 64, which is parked at the inlet 65, to the sampling position along the Y direction, and pushes the sample rack back to the sample rack placement slot 64 after sampling.

[0038] Combination Figure 3 The Y-axis insertion assembly includes a second mounting frame 66, a hook rod 67, and an insertion drive component 68. The insertion drive component 68 is mounted on the second mounting frame 66. Driven by the insertion drive component 68, the hook rod 67 reciprocates along the Y direction, hooking the sample holder located at the insertion port 65 to the sampling position or returning it to the sample holder placement slot 64. Specifically, as... Figure 4As shown, the bottom of the sample holder is provided with a straight groove 69 corresponding to the stroke position of the hook rod 67, which facilitates the insertion and hooking of the hook rod 67. The hook rod 67 is a rod-shaped rod extending in a vertical direction. The straight groove 69 is provided along the X direction. When the hook rod 67 moves to the working position, it is located below the inlet 65. The sample holder moves to the inlet 65 along with the sample tray 62. The straight groove 69 of the sample holder is exactly matched to the insertion of the hook rod 67. The inlet drive 68 drives the hook rod 67 to move along the Y direction to the sampling position for sampling. After sampling, the inlet drive 68 drives the hook rod 67 to move in the opposite direction along the Y direction to the initial position. The sample tray 62 moves to the next sample holder to be sampled, corresponding to the position of the inlet 65, under the action of the sample feeding drive 63.

[0039] The sample injection drive 63 and the rack drive 68 both adopt a structure of motor, drive wheel, driven wheel and conveyor belt. In the sample injection X assembly, the sample tray 62 is connected to the conveyor belt through a connector and is driven to move back and forth by the rack drive. In the sample injection Y assembly, the hook rod 67 is connected to the conveyor belt through a connector and is driven to move back and forth in the Y direction by the sample injection drive.

[0040] In this embodiment, as Figure 2 The mounting frame 61 includes a base plate 611 and a rear side plate 612. The sample tray 62 is slidably mounted on the base plate 611 via a slide rail. A buffer plate 613 is provided on the rear side plate 612. When the sample rack is placed into the sample rack placement slot 64, if the sample rack slides too far backward and touches the buffer plate 613, it will gradually return to its position and stabilize under the action of the buffer plate.

[0041] In this embodiment, the mixing mechanism 8 is as follows: Figure 5 and Figure 6 As shown, the device includes a mixing drive 81, a mixing assembly 82, and a rotation limiting assembly 83. The mixing assembly 82 includes a rotating seat 821 with a cup holder 822 on it. The rotating seat 821 is eccentrically positioned relative to the drive shaft 811 of the mixing drive 81, with the eccentricity d between the rotating seat 821 and the drive shaft 811 ranging from 1 mm to 4 mm. Figure 7The rotation limiting assembly 83 includes a bearing housing 831, which has a vertical mounting hole 8311 and a horizontal mounting hole 8312. The bearing housing 831 is rotatably mounted vertically through the vertical mounting hole 8311. A limiting shaft 832 is slidably disposed in the horizontal mounting hole 8312, and the other end of the limiting shaft 832 is connected to a rotating seat 821. Specifically, a sleeve 833 is interference-fitted into the horizontal mounting hole 8312, and the limiting shaft 832 is slidably disposed within the sleeve 833. The rotation limiting assembly 83 also includes a limiting post 834 and a rotation limiting seat 835. Two bearings are installed in the vertical mounting hole 8311, located at the upper and lower ends of the vertical mounting hole 8311, respectively. The upper end of the limiting post 834 is interference-fitted onto the inner rings of the two bearings, and the lower end of the limiting post 834 is installed in the rotation limiting seat 835. The corresponding rotation limiting seat 835 has a hole that matches the lower end of the limiting post 834. The mixing mechanism 8's mixing drive 81 also adopts a structure of motor, drive wheel, driven wheel, and conveyor belt. Other structures, such as sensor components, adopt structural methods that can be realized in the prior art.

[0042] The sample dispensing mechanism 1 in this embodiment is as follows: Figure 8 As shown, it includes a liquid-collecting needle 11 for drawing or discharging liquid, a rotating part 12 for driving the liquid-collecting needle to rotate circumferentially, and a lifting part 13 for driving the liquid-collecting needle to move up and down. The specific structure of each component is the same as that of the prior art. The structure and working principle of the reagent adding mechanism 2 are the same as those of the sample adding mechanism. The incubation mechanism 3 is as follows... Figure 9 As shown, it includes an inner incubation disk 31 with multiple incubation holes for accommodating reaction cups, an inner disk drive 32 for driving the inner incubation disk, an outer incubation disk 33 for maneuvering the reaction cups, and an outer disk drive 34 for driving the outer incubation disk.

[0043] The magnetic separation cleaning mechanism 4 in this embodiment is as follows: Figure 10 and Figure 11 As shown, the system includes a reaction cup rotary motor 41 for scheduling the reaction cup at the injection and waste discharge positions, an injection needle 42 for injecting cleaning fluid into the reaction cup and an injection needle lifting assembly for controlling the lifting and lowering of the injection needle, a mixing head 43 for mixing the reactants in the reaction cup and a mixing head lifting motor 44 for controlling the lifting and lowering of the mixing head and a mixing head rotary motor 45 for controlling the rotation of the mixing head, and a waste discharge needle 46 for emptying the waste liquid in the reaction cup and a waste discharge needle lifting motor 47 for controlling the lifting and lowering of the waste discharge needle.

[0044] The reagent storage mechanism 5 in this embodiment is as follows: Figure 12As shown, the device includes a storage tray 51 for storing reagent kits, a tray cover 52 that covers the storage tray, and a storage tray rotation mechanism 53 for controlling the rotation of the storage tray. The mechanism includes a fixed gear for mixing magnetic bead bottles (the magnetic bead bottles mesh with the fixed gear after being placed in the storage tray, and the rotation of the tray and the rotation of the magnetic bead bottles achieves mixing of the magnetic beads). The bottom of the reagent tray has a Peltier and a heat sink. The cold end of the Peltier is in contact with the reagent tray body, and the hot end is in contact with the heat sink. The heat dissipation component 54 dissipates the heat from the hot end to the outside of the machine, so that the internal temperature of the reagent tray reaches 2-8 degrees Celsius when it is in a stable state.

[0045] Photoelectric testing institutions 7 Figure 13 As shown, it includes a rotating detection disk 71 for storing the reaction cup and a drive mechanism 72 for controlling the rotation of the rotating detection disk, an excitation liquid injection hole 73 for injecting excitation liquid, a photomultiplier tube assembly 74 for measuring the light in the reaction cup, a drain needle 75 for draining waste liquid in the reaction cup and a drive mechanism 76 for controlling the raising and lowering of the drain needle.

[0046] The reaction cup sorting mechanism 9 in this embodiment is as follows: Figure 14 As shown, the system includes a hopper 91 for storing reaction cups, a lifting plate 92 for lifting the reaction cups, a lifting plate drive mechanism 93 for driving the lifting plate, a slide 94 for orienting and buffering the reaction cups, a rotating cup disk 95 for scheduling the reaction cups, and a rotating cup disk drive mechanism 96 for controlling the rotation of the scheduling disk. The cup-gripping hand mechanism 10 in this embodiment is as follows... Figure 15 As shown, it includes a cup-grabbing hand 101 for picking up and placing reaction cups, a cup-grabbing motor 102 for controlling the switch of the cup-grabbing hand, a rotation drive assembly 103 for driving the cup-grabbing hand to rotate circumferentially, and a lifting drive assembly 104 for driving the cup-grabbing hand to move up and down.

[0047] Structures not specifically described in this application can be implemented using existing technologies. For example, driving components can be implemented in the form of motors, driving wheels, driven wheels, or conveyor belts, or other driving methods can be used.

Claims

1. A chemiluminescence immunoassay analyzer, characterized in that, Includes a frame, and on the frame are a sample addition mechanism (1), a reagent addition mechanism (2), an incubation mechanism (3), a magnetic separation and cleaning mechanism (4), a reagent storage mechanism (5), a sample injection mechanism (6), a photoelectric detection mechanism (7), a mixing mechanism (8), a reaction cup handling mechanism (9), and a cup gripping mechanism (10). The reagent storage mechanism (5) is arranged on one side of the analyzer, the sample injection mechanism (6) is arranged on the proximal side of the reagent storage mechanism (5), the reagent storage mechanism (5) is provided with an incubation mechanism (3) and a magnetic separation and cleaning mechanism (4) in sequence on the other side of the analyzer, and a reaction cup sorting mechanism (9) is arranged on the proximal side of the magnetic separation and cleaning mechanism (4). The sample addition mechanism (1) is located between the sample injection mechanism (6) and the incubation mechanism (3) and is used to add the sample to the incubation mechanism (3); the reagent addition mechanism (2) is located between the reagent storage mechanism (5) and the incubation mechanism (3) and is used to add the reagent to the incubation mechanism (3). The cup-grabbing mechanism (10) is located between the magnetic separation and cleaning mechanism (4) and the incubation mechanism (3). The photoelectric detection mechanism (7) and the mixing mechanism (8) are both located between the reaction cup sorting mechanism (9) and the incubation mechanism (3) and are within the stroke range of the cup-grabbing mechanism (10), so that the cup-grabbing mechanism (10) can pick up and put down the reaction cup between the magnetic separation and cleaning mechanism (4), the incubation mechanism (3) and the photoelectric detection mechanism (7).

2. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The sample addition mechanism (1), reagent addition mechanism (2), incubation mechanism (3), magnetic separation and cleaning mechanism (4), reagent storage mechanism (5), sample injection mechanism (6), photoelectric detection mechanism (7), mixing mechanism (8), reaction cup sorting mechanism (9), and cup gripping mechanism (10) are all located on the same plane of the frame.

3. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The sample injection mechanism (6) includes an X-component for injection and a Y-component for mounting. The X-component includes a mounting frame (61), a sample tray (62), and an injection drive (63). The sample tray (62) is mounted on the mounting frame (61) via a slide rail and reciprocates in the X direction under the drive of the injection drive (63). The sample tray (62) has a sample rack placement slot (64) that runs through it. The mounting frame (61) has an entry port (65), and the Y-component for mounting is installed at the position corresponding to the entry port (65). The Y-component for mounting is used to move the sample rack located in the sample placement slot (64) corresponding to the entry port (65) to the sampling position along the Y direction, and pushes the sample rack back to the sample rack placement slot (64) after sampling.

4. The chemiluminescence immunoassay analyzer according to claim 3, characterized in that, The Y-assembly for feeding the sample rack includes a second mounting frame (66), a hook rod (67), and a feeding rack drive (68). The feeding rack drive (68) is mounted on the second mounting frame (66). The hook rod (67) moves back and forth along the Y direction under the drive of the feeding rack drive (68) and hooks the sample rack located at the feeding rack inlet (65) to the sampling position or sends it back to the sample rack placement slot (64).

5. The chemiluminescence immunoassay analyzer according to claim 4, characterized in that, The sample holder is provided with a through groove (69) at the position corresponding to the stroke of the hook rod (67) to facilitate the hook rod (67) to extend and hook.

6. The chemiluminescence immunoassay analyzer according to claim 3, characterized in that, The mounting bracket (61) includes a base plate (611) and a rear side plate (612). The sample tray (62) is slidably mounted on the base plate (611) via a slide rail. A buffer plate (613) is provided on the rear side plate (612).

7. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The mixing mechanism (8) includes a mixing drive (81) and a mixing assembly (82). The mixing assembly (82) includes a rotating seat (821) with a cup holder (822) on it. The rotating seat (821) is eccentrically positioned with respect to the drive shaft (811) of the mixing drive (81).

8. The chemiluminescence immunoassay analyzer according to claim 7, characterized in that, The mixing mechanism (8) further includes a rotation limiting component (83), which includes a bearing seat (831). The bearing seat (831) is provided with a vertical mounting hole (8311) and a horizontal mounting hole (8312). The bearing seat (831) is rotatably mounted in the vertical direction through the vertical mounting hole (8311). A limiting shaft (832) is slidably provided in the horizontal mounting hole (8312). The other end of the limiting shaft (832) is connected to a rotating seat (821).

9. The chemiluminescence immunoassay analyzer according to claim 7, characterized in that, The eccentricity d between the rotating seat (821) and the drive shaft (811) of the mixing drive (81) is 1 mm to 4 mm.

Images