Cell stationary liquid device for flow detection

Abstract

The utility model discloses a cell stationary liquid device for flow type detection, which comprises a magnetic stirrer, a mixing bottle arranged at the top of the magnetic stirrer, a sample inlet arranged at the top of the mixing bottle, a limit cylinder arranged at the top of the inner side of a mounting rack, and a nozzle connected with the inner side of the limit cylinder through a driving mechanism, one side of the spray head is connected with the micro-flow pump through a hose, the other end of the micro-flow pump is connected with the bottom of the light-proof liquid storage tank through a hose, and a control panel is mounted on one side of the mounting frame, the light-proof liquid storage tank can prevent degradation of photosensitive stationary liquid, and the micro-flow pump can extract the stationary liquid and inject the stationary liquid into the mixing bottle through the spray head; the driving mechanism can push the spray head to be automatically connected with the sample inlet in an inserted mode, the magnetic stirrer can drive the stirrer in the mixing bottle to rotate, so that stationary liquid and cell suspension are evenly mixed, efficient, accurate and pollution-free flow cytometry cell fixing operation is achieved through the structure, and experiment repeatability and the cell survival rate are remarkably improved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical detection technology, specifically a cell fixation solution device for flow cytometry detection. Background Technology

[0002] When performing flow cytometry in the laboratory, cells are usually directly fed into the instrument after staining to avoid prolonged storage. Prolonged storage can lead to cell breakage and antibody fluorescence quenching, resulting in increased cell debris, inaccurate data, and decreased fluorescence intensity. However, if the laboratory has a large sample volume or lacks a flow cytometer and needs to send samples to a third-party laboratory, prolonged storage of the samples is unavoidable. Conversely, frequent turning the instrument on and off when the sample volume is small can waste instruments and reagents. Therefore, it is generally advisable to collect the samples for unified testing, which also requires a period of storage.

[0003] In flow cytometry, the role of cell fixative is to maintain cell morphology and stabilize intracellular components to ensure the accuracy of detection data. Traditional fixatives have the following problems: they need to be prepared manually, which can easily introduce errors or contamination; they are also prone to volatilization or oxidation when exposed to air, affecting the results; and manual sample addition may lead to insufficient or over-fixation of cells. Therefore, it is necessary to design a cell fixative device for flow cytometry to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a cell fixation solution device for flow cytometry detection, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell fixative device for flow cytometry detection, comprising a magnetic stirrer, a mixing bottle placed on top of the magnetic stirrer, and a stir bar pre-installed inside the bottle; a sample inlet on top of the mixing bottle; a mounting frame connected to the top of the magnetic stirrer via a stand; a light-proof storage tank mounted on top of the mounting frame; a limiting cylinder on the inner top of the mounting frame; a nozzle slidably connected to the inner side of the limiting cylinder; a driving mechanism on one side of the limiting cylinder to facilitate the insertion of the nozzle into the sample inlet; a through groove on one side of the limiting cylinder; a flexible tube passing through the inner side of the through groove; one end of the flexible tube connected to the nozzle; the other end of the flexible tube connected to one end of a micro-flow pump; the other end of the micro-flow pump connected to the bottom of the light-proof storage tank via the flexible tube; and a control panel mounted on one side of the mounting frame.

[0006] Preferably, the driving mechanism includes a drive motor, a movable groove is provided on one side of the limiting cylinder, a connecting block is slidably connected to the inner side of the movable groove, one end of the connecting block is connected to the nozzle, the other end of the connecting block is connected to a connecting plate, an abutment groove is provided on one side of the connecting plate, the drive motor is installed on one side of the mounting frame, the output end of the drive motor passes through the interior of the mounting frame and is connected to a turntable, and an abutment rod adapted to the abutment groove is installed on one side of the turntable near the edge.

[0007] Preferably, the inner wall of the light-proof liquid storage tank is coated with an anti-oxidation coating, the top of the light-proof liquid storage tank is provided with a liquid injection port, and a scale is provided on one side of the light-proof liquid storage tank.

[0008] Preferably, the flow rate adjustment range of the micro-flow pump is 0.1–5 ml / min.

[0009] Preferably, the mixing bottle is made of a transparent material.

[0010] Preferably, the injection port is an elastic silicone sealing gasket with a thickness of 2-3 mm and a cross-shaped cut in the center.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. This utility model relates to a light-shielding storage tank for storing cell fixatives. Its light-shielding design prevents degradation of the photosensitive fixative. A micro-flow pump draws the fixative from the light-shielding storage tank through a flexible tube and precisely injects it into a mixing bottle via a nozzle. A drive mechanism pushes the nozzle to slide along a limiting cylinder and automatically connects to the sample inlet, ensuring a tight seal during sample loading. A magnetic stirrer drives the stir bar inside the mixing bottle to rotate, ensuring uniform mixing of the fixative and cell suspension. The control panel coordinates the actions of the micro-flow pump, drive mechanism, and magnetic stirrer, achieving full automation of the process. Thus, through the above structure, efficient, accurate, and pollution-free flow cytometry cell fixation operations are achieved, significantly improving experimental repeatability and cell viability. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0014] Figure 2 This is a bottom view of the present invention;

[0015] Figure 3 This is a side view and a top view of the present invention;

[0016] Figure 4 This is a side sectional view of the present invention;

[0017] Figure 5 for Figure 4 Enlarged view of part A in the image.

[0018] In the diagram: 1. Magnetic stirrer, 2. Mixing bottle, 3. Sample inlet, 4. Stand, 5. Mounting bracket, 6. Light-proof storage tank, 7. Injection port, 8. Scale, 9. Limiting cylinder, 10. Nozzle, 11. Through groove, 12. Hose, 13. Micro-flow pump, 14. Movable groove, 15. Connecting block, 16. Connecting plate, 17. Contact groove, 18. Drive motor, 19. Turntable, 20. Contact rod, 21. Control panel. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example 1

[0021] Please refer to Figure 1-5 As shown, this utility model provides a cell fixative device for flow cytometry detection, including a magnetic stirrer 1, a mixing bottle 2 placed on top of the magnetic stirrer 1, and a stir bar pre-installed inside the bottle. The top of the mixing bottle 2 is provided with a sample inlet 3. The top of the magnetic stirrer 1 is connected to a mounting frame 5 via a stand 4. A light-proof storage tank 6 is installed on the top of the mounting frame 5. A limiting cylinder 9 is provided on the inner top of the mounting frame 5. A nozzle 10 is slidably connected to the inner side of the limiting cylinder 9. A driving mechanism is provided on one side of the limiting cylinder 9 to facilitate the insertion of the nozzle 10 into the sample inlet 3. A through groove 11 is opened on one side of the limiting cylinder 9. A flexible tube 12 passes through the inner side of the through groove 11. One end of the flexible tube 12 is connected to the nozzle 10, and the other end of the flexible tube 12 is connected to one end of a micro-flow pump 13. The other end of the micro-flow pump 13 is connected to the bottom of the light-proof storage tank 6 via the flexible tube 12. A control panel 21 is installed on one side of the mounting frame 5.

[0022] Specifically, the light-proof storage tank 6 is used to store cell fixative. Its light-proof design can prevent the degradation of photosensitive fixative. The micro-flow pump 13 can draw fixative from the light-proof storage tank 6 through the hose 12 and inject it precisely into the mixing bottle 2 through the nozzle 10. The drive mechanism can push the nozzle 10 to slide along the limiting cylinder 9 and automatically connect with the sample inlet 3 to ensure the sample addition is sealed. The magnetic stirrer 1 can drive the stir bar in the mixing bottle 2 to rotate, so that the fixative and cell suspension are mixed evenly. The control panel 21 can coordinate the actions of the micro-flow pump 13, the drive mechanism and the magnetic stirrer 1 to realize the full-process automation. Thus, through the above structure, the flow cytometry cell fixation operation is made efficient, accurate and pollution-free, significantly improving the experimental repeatability and cell viability.

[0023] The driving mechanism includes a drive motor 18. A movable groove 14 is provided on one side of the limiting cylinder 9. A connecting block 15 is slidably connected to the inner side of the movable groove 14. One end of the connecting block 15 is connected to the nozzle 10, and the other end is connected to a connecting plate 16. A contact groove 17 is provided on one side of the connecting plate 16. The drive motor 18 is mounted on one side of the mounting frame 5. The output end of the drive motor 18 passes through the interior of the mounting frame 5 and is connected to a turntable 19. A contact rod 20, adapted to the contact groove 17, is installed near the edge of one side of the turntable 19. By starting the drive motor 18, the drive motor 18 can drive the turntable 19 to rotate. The contact rod 20 on the turntable 19 moves in a circular motion with the turntable 19. When the contact rod 20 contacts... When the contact groove 17 on the connecting plate 16 is reached, the contact groove 17 is pushed to move, which in turn drives the connecting plate 16, the connecting block 15, and the nozzle 10 to slide along the movable groove 14 toward the sample inlet 3. The movement of the nozzle 10 is continuously observed. When the nozzle 10 is accurately inserted into the sample inlet 3 of the mixing bottle 2, the rotation of the drive motor 18 is stopped, and the insertion operation of the nozzle 10 is completed. After the nozzle 10 is inserted into the sample inlet 3, the drive motor 18 is kept stopped to ensure that the nozzle 10 is stably kept in the sample inlet 3. At this time, the subsequent operation can be carried out according to the workflow of other parts of the device. Through the settings, the precise docking and separation of the nozzle 10 and the sample inlet 3 can be achieved, ensuring the accuracy and efficiency of the fixative addition operation.

[0024] The inner wall of the light-proof liquid storage tank 6 is coated with an anti-oxidation coating. The top of the light-proof liquid storage tank 6 is equipped with a liquid inlet 7, and a scale 8 is provided on one side of the light-proof liquid storage tank 6. The light-proof liquid storage tank 6 is made of opaque material, which can prevent the photosensitive fixative from decomposing and failing under light. The anti-oxidation coating can block oxygen penetration and extend the shelf life of the fixative. The liquid inlet 7 allows for easy addition of fixative and prevents evaporation. The scale 8 can display the remaining liquid volume in real time and supports accurate dispensing.

[0025] The flow rate of the micro-flow pump 13 is adjustable from 0.1 to 5 ml / min, and can be set to achieve high-precision micro-volume sample addition.

[0026] Among them, the mixing bottle 2 is made of transparent material, which allows for easy observation of the mixing state of the fixative and cell suspension.

[0027] The inlet 3 is a flexible silicone sealing gasket with a thickness of 2-3 mm and a cross-shaped cut in the center. The flexible silicone has good elasticity and flexibility, and its 2-3 mm thickness allows it to deform under external force and return to its original shape after the external force is removed. When the nozzle 10 is inserted into the inlet 3, the silicone sealing gasket will tightly adhere to the surface of the nozzle 10 to form a seal, preventing liquid leakage in the mixing bottle 2 and the entry of outside air into the bottle, ensuring the stability of the internal environment of the mixing bottle 2, and avoiding interference from external factors during the mixing process of the cell fixative. The cross-shaped cut in the center will automatically open when the nozzle 10 is inserted to provide a channel for the nozzle 10. When the nozzle 10 is pulled out, due to the elasticity of the silicone sealing gasket, the cross-shaped cut will automatically close, returning to the initial sealed state, continuing to maintain the seal of the mixing bottle 2, preventing the liquid inside the bottle from evaporating and external impurities from entering.

[0028] Working principle: First, the flow rate of the micro-flow pump 13 and the amount of fixative added can be set through the control panel 21. By starting the drive mechanism, the nozzle 10 can slide inside the limiting cylinder 9, so that the nozzle 10 is inserted into the sample inlet 3 of the mixing bottle 2. By starting the micro-flow pump 13, the fixative in the light-proof storage tank 6 can be delivered to the nozzle 10 through the hose 12 at the set flow rate, and then injected into the mixing bottle 2 by the nozzle 10. At the same time or after the fixative is injected into the mixing bottle 2, the magnetic stirrer 1 is started. The magnetic device inside the magnetic stirrer 1 generates a rotating magnetic field, which drives the stir bar in the mixing bottle 2 to rotate, so that the cell sample and fixative are fully mixed, thereby completing the entire operation process.

[0029] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0030] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A cell fixative device for flow cytometry detection, comprising a magnetic stirrer (1), characterized in that: A mixing bottle (2) is placed on top of the magnetic stirrer (1), and a stir bar is pre-installed inside the bottle. The top of the mixing bottle (2) is provided with a sample inlet (3). The top of the magnetic stirrer (1) is connected to a mounting bracket (5) via a stand (4). A light-proof liquid storage tank (6) is installed on the top of the mounting bracket (5). A limiting cylinder (9) is provided on the inner top of the mounting bracket (5). A nozzle (10) is slidably connected to the inner side of the limiting cylinder (9). One side of the limiting cylinder (9) is provided to facilitate the movement of the nozzle (10) with the... The drive mechanism is connected to the inlet (3). A through groove (11) is provided on one side of the limiting cylinder (9). A hose (12) passes through the inside of the through groove (11). One end of the hose (12) is connected to the nozzle (10). The other end of the hose (12) is connected to one end of the micro-flow pump (13). The other end of the micro-flow pump (13) is connected to the bottom of the light-proof liquid storage tank (6) through the hose (12). A control panel (21) is installed on one side of the mounting bracket (5).

2. The cell fixation solution device for flow cytometry detection according to claim 1, characterized in that: The driving mechanism includes a drive motor (18), a movable groove (14) is provided on one side of the limiting cylinder (9), a connecting block (15) is slidably connected to the inner side of the movable groove (14), one end of the connecting block (15) is connected to the nozzle (10), the other end of the connecting block (15) is connected to a connecting plate (16), a contact groove (17) is provided on one side of the connecting plate (16), the drive motor (18) is installed on one side of the mounting bracket (5), the output end of the drive motor (18) passes through the interior of the mounting bracket (5) and is connected to a turntable (19), and a contact rod (20) that matches the contact groove (17) is installed on one side of the turntable (19) near the edge.

3. The cell fixative device for flow cytometry detection according to claim 1, characterized in that: The inner wall of the light-proof liquid storage tank (6) is coated with an anti-oxidation coating. The top of the light-proof liquid storage tank (6) is provided with a liquid injection port (7). A scale (8) is provided on one side of the light-proof liquid storage tank (6).

4. The cell fixation solution device for flow cytometry detection according to claim 3, characterized in that: The flow rate adjustment range of the micro-flow pump (13) is 0.1–5 ml / min.

5. A cell fixative device for flow cytometry detection according to claim 4, characterized in that: The mixing bottle (2) is made of transparent material.

6. A cell fixation solution device for flow cytometry detection according to claim 5, characterized in that: The injection port (3) is an elastic silicone sealing gasket with a thickness of 2-3 mm and a cross-shaped cut in the center.

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