An integrated device for in-situ detection of microorganisms

Abstract

This utility model discloses an integrated device for in-situ microbial detection, specifically relating to the field of water body testing technology. The integrated device for in-situ microbial detection includes: a base plate with four casters fixed to its bottom; a power supply and four first support pillars fixed to the base plate; a middle plate fixedly mounted on top of the four first support pillars; a magnetic frame and a centrifugal vibrating device fixedly mounted on top of the middle plate; two second support pillars and two third support pillars disposed on top of the middle plate; and two fixing plates disposed above the middle plate, with the two fixing plates respectively fixed to the two second support pillars and the two third support pillars. A top plate is disposed between the two fixing plates. The integrated device for in-situ microbial detection provided by this utility model enables fully outdoor operation, avoiding sample distortion caused by laboratory transport.

Description

Technical Field

[0001] This utility model relates to the field of water body detection technology, and in particular to an integrated device for in-situ detection of microorganisms. Background Technology

[0002] With the intensification of global climate change and human activities, aquatic ecosystems are facing unprecedented challenges. As an important component of aquatic ecosystems, changes in the community structure and function of microorganisms directly reflect the health status of water bodies.

[0003] Traditional microbial detection methods typically require water samples to be collected in a laboratory for analysis. During sampling, transportation, and laboratory processing, the activity, quantity, and community structure of microorganisms may change significantly, leading to discrepancies between the test results and the actual situation. This makes it difficult for traditional methods to achieve real-time monitoring and continuous observation, and to capture the dynamic changes in the microbial community in a timely manner.

[0004] Therefore, it is necessary to provide an integrated device for in-situ detection of microorganisms to solve the above-mentioned technical problems. Utility Model Content

[0005] In view of the above situation and to overcome the defects of the existing technology, this utility model provides an integrated device for in-situ detection of microorganisms that can realize the entire process of outdoor operation and avoid sample distortion caused by laboratory transportation.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] An integrated device for in-situ microbial detection includes: a base plate with four casters fixed to the bottom; a power supply and four first pillars fixed on the base plate; a middle plate fixedly installed on the top of the four first pillars; a magnetic frame and a shaking centrifuge device fixedly installed on the top of the middle plate; two second pillars and two third pillars arranged on the top of the middle plate; two fixing plates arranged above the middle plate, which are respectively fixed to the two second pillars and the two third pillars; a top plate arranged between the two fixing plates; and a Nanopore third-generation sequencer, a Qubit fluorescence quantitative quantitation instrument, and a laptop micro workstation fixedly installed on the top plate. The power supply provides power to each device.

[0008] Preferably, the integrated device for in-situ detection of microorganisms further includes a protective cover, which is fixed to the first support and the top plate by multiple screws.

[0009] Preferably, the base plate has a spiral groove, which is used to limit the movement of the protective cover.

[0010] Preferably, a rotating shaft is fixed on both sides of the top plate, and the two rotating shafts are rotatably connected to the corresponding fixed plates.

[0011] Preferably, a connecting plate is rotatably mounted on one side of the fixed plate, and the connecting plate is fixed to the top plate by screws.

[0012] Preferably, two fixed rectangular rods are fixedly installed at the bottom of the two second pillars, and two movable rectangular rods are provided on the two third pillars. Two rectangular holes are opened on the base plate, and the rectangular holes are adapted to the fixed rectangular rods. Four rectangular slots are opened on the top of the middle plate, and the fixed rectangular rods and the movable rectangular rods are adapted to the rectangular slots. When the two fixed rectangular rods and the two movable rectangular rods are respectively located in the corresponding rectangular slots, the entire device is in a highly superimposed state; when the fixed rectangular rods are located in the two rectangular holes, the entire device is in a flat state.

[0013] Preferably, a sliding groove is provided at the bottom end of the third support column, the movable rectangular rod is slidably installed inside the sliding groove, and a limiting groove is provided on the side of the two third supports columns that are close to each other. The sliding groove and the limiting groove are connected. A lead screw is fixedly installed on one side of the movable rectangular rod, the lead screw passes through the limiting groove, and a nut is threaded on the outside of the limiting groove.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) This utility model integrates a power supply, magnetic rack, oscillating centrifuge device, Nanopore third-generation sequencer, Qubit fluorescence quantitative instrument and notebook micro workstation into an integrated device to realize the whole process of "sampling-pretreatment-detection-analysis" for outdoor operation, and avoids sample distortion caused by laboratory transportation.

[0016] (2) This utility model supports rapid transfer to the field environment through the design of universal wheel chassis and flip-top plate, which can meet the on-site detection needs of sudden pollution events or extreme environments.

[0017] (3) This utility model adopts a multi-layer design and combines a rectangular rod limiting system to realize the flexible conversion between the equipment stacked transportation state and the flat operation state. Attached Figure Description

[0018] Figure 1 A schematic diagram of the integrated device for in-situ microbial detection provided by this utility model;

[0019] Figure 2 A schematic diagram of the integrated device for in-situ microbial detection provided by this utility model;

[0020] Figure 3 for Figure 1 A schematic diagram of the protective cover in the integrated device for in-situ microbial detection shown;

[0021] Figure 4 for Figure 1 The diagram shows the internal structure of the integrated device for in-situ microbial detection.

[0022] Figure 5 This is a structural schematic diagram of the base plate;

[0023] Figure 6 This is a schematic diagram of the structure after the top plate is flipped over;

[0024] Figure 7 This is a structural schematic diagram of the top slab;

[0025] Figure 8 This is a partial structural diagram of the device;

[0026] Figure 9 This is a schematic diagram of the device in a flat state;

[0027] Figure 10 This is a cross-sectional structural diagram of the third pillar.

[0028] The corresponding names of the attached figures are as follows: 1-base plate, 2-power supply, 3-first support column, 4-intermediate plate, 5-magnetic frame, 6-oscillating centrifuge device, 7-second support column, 8-third support column, 9-fixed plate, 10-top plate, 11-Nanopore third-generation sequencer, 12-Qubit fluorescence quantitative quantitation instrument, 13-laptop micro workstation, 14-protective cover, 15-U-shaped groove, 16-connecting plate, 17-fixed rectangular rod, 18-rectangular hole, 19-rectangular groove, 20-movable rectangular rod, 21-slide groove, 22-limiting groove, 23-lead screw, 24-nut. Detailed Implementation

[0029] The present invention will be further described below with reference to the accompanying drawings and embodiments. The embodiments of the present invention include, but are not limited to, the following embodiments.

[0030] Example 1

[0031] like Figure 1-10As shown, this utility model provides an integrated device for in-situ microbial detection, comprising: a base plate 1 with four casters fixed to the bottom; a power supply 2 and four first support pillars 3 fixed on the base plate 1; a middle plate 4 fixedly installed on the top of the four first support pillars 3; a magnetic frame 5 and a shaking centrifuge device 6 fixedly installed on the top of the middle plate 4; two second support pillars 7 and two third support pillars 8 arranged on the top of the middle plate 4; two fixing plates 9 arranged above the middle plate 4, the two fixing plates 9 being fixed to the two second support pillars 7 and the two third support pillars 8 respectively; a top plate 10 arranged between the two fixing plates 9; and a Nanopore third-generation sequencer 11 and a Qubit fluorescence quantitative quantitation device fixedly installed on the top plate 10. The Nanopore third-generation sequencer 12 and the notebook micro-workstation 13 are powered by a power supply 2. The notebook micro-workstation 13 includes a high-resolution touchscreen and input components, an Intel Core i9 processor, 32GB of RAM, and a 2TB solid-state drive. It supports a dual Linux system, has data storage management and analysis functions, and can run bioinformatics analysis software and handle multitasking and complex calculations. The Nanopore third-generation sequencer 11 measures 160mm*115mm*30mm and consists of an OLED touchscreen and a SpotOn sequencing chip. The SpotOn sequencing chip has SpotOn sample wells for adding pre-processed sequencing libraries, allowing direct processing of raw DNA or RNA. Sequencing capabilities include read lengths from 200 bp to 2 Mb, with real-time data acquisition and rapid result generation. Its single-molecule real-time sequencing technology can be used in various research fields such as whole-genome sequencing, RNA sequencing, and metagenomic sequencing. The Qubit fluorescence quantitative quantitation instrument 12 measures 135 mm * 25 mm * 5.5 mm and consists of a touchscreen display and optical detection components. It supports sample volumes of 1-20 μL to meet diverse experimental requirements, with each sample processing time less than 5 seconds, significantly improving experimental efficiency and meeting the needs of in-situ measurements outside the laboratory. The magnetic rack 5 consists of a magnet assembly and a support. The magnet assembly is composed of high-performance neodymium iron boron magnets, capable of generating a strong magnetic field for adsorbing and processing samples. The device utilizes magnetic microbeads, whose powerful magnetic field ensures efficient adsorption of magnetic materials, reducing sample loss and improving experimental efficiency. The support is compatible with 1.5-2ml centrifuge tubes, used to fix the magnet assembly and place the centrifuge tubes; each side can hold 10 centrifuge tubes, with a maximum of 20 tubes at a time. This device integrates a power supply (2), magnetic support (5), shaking centrifuge device (6), Nanopore third-generation sequencer (11), Qubit fluorescence quantitative quantitation system (12), and a laptop workstation (13), allowing for convenient outdoor transport for real-time monitoring and continuous detection. This avoids potential changes in microbial activity, quantity, and community structure during transportation and laboratory processing, thus preventing discrepancies between test results and actual conditions.

[0032] Example 2

[0033] like Figure 1-3 As shown, the integrated device for in-situ detection of microorganisms also includes a protective cover 14, which is fixed to the first support column 3 and the top plate 10 by multiple screws. The protective cover 14 protects the power supply 2, the magnetic rack 5, the shaking centrifuge device 6, the Nanopore third-generation sequencer 11, the Qubit fluorescence quantitative instrument 12, and the laptop micro workstation 13.

[0034] Furthermore, a spiral groove 15 is provided on the base plate 1. The spiral groove 15 is used to limit the position of the protective cover 14. When the protective cover 14 is installed, the protective cover 14 is first inserted into the spiral groove 15, and then the protective cover 14 is fixed with screws.

[0035] Furthermore, two rotating shafts are fixed on both sides of the top plate 10, and the two rotating shafts are respectively rotatably connected to the corresponding fixed plates 9. When the entire device is under the protection of the protective cover 14 (e.g., Figure 4 As shown), the equipment on the top plate 10 is positioned downwards. However, during testing, the top plate 10 needs to be rotated 180° to flip the downward-facing equipment upwards (as shown). Figure 6 (As shown), so that personnel can conduct water sample testing.

[0036] Furthermore, a connecting plate 16 is rotatably mounted on one side of the fixing plate 9. The connecting plate 16 is fixed to the top plate 10 by screws. The connecting plate 16, in conjunction with the screws, ensures that the top plate 10 is in a fixed state.

[0037] Example 3

[0038] like Figure 8-10 As shown, two fixed rectangular rods 17 are fixedly installed at the bottom of the two second pillars 7, and two movable rectangular rods 20 are provided on the two third pillars 8. Two rectangular holes 18 are opened on the base plate 1, which are adapted to the fixed rectangular rods 17. Four rectangular slots 19 are opened at the top of the intermediate plate 4, and both the fixed rectangular rods 17 and the movable rectangular rods 20 are adapted to the rectangular slots 19. The design of the rectangular holes 18 and rectangular slots 19 allows the device to achieve two forms of conversion. When the two fixed rectangular rods 17 and the two movable rectangular rods 20 are respectively located in the corresponding rectangular slots 19, the entire device is in a highly superimposed state (e.g., ...). Figure 6 As shown), this state facilitates transportation; when the fixed rectangular rod 17 is located in the two rectangular holes 18, the entire device is in a flat state (as shown). Figure 9 As shown in the figure, this state is convenient for personnel to operate, and users can switch the state of the device according to the specific usage environment.

[0039] Furthermore, a sliding groove 21 is provided at the bottom of the third support column 8, and the movable rectangular rod 20 is slidably installed inside the sliding groove 21. Limiting grooves 22 are provided on the sides of the two third support columns 8 that are close to each other. The sliding groove 21 and the limiting groove 22 are connected. A lead screw 23 is fixedly installed on one side of the movable rectangular rod 20. The lead screw 23 passes through the limiting groove 22, and a nut 24 is threaded on the outside of the limiting groove 22. Through the above design, the position of the movable rectangular rod 20 can be adjusted, so that the height of the third support column 8 and the movable rectangular rod 20 can adapt to the height of the device after it is laid flat. In specific adjustment, first loosen the nut 24, then adjust the movable rectangular rod 20 to a suitable height, and finally tighten the nut 24.

Claims

1. A device for in-situ detection of microorganisms, characterized by comprising: include: A base plate (1) with four casters is fixed at the bottom. A power supply (2) and four first pillars (3) are fixed on the base plate (1). A middle plate (4) is fixedly installed on the top of the four first pillars (3). A magnetic frame (5) and a oscillating centrifuge device (6) are fixedly installed on the top of the middle plate (4). Two second pillars (7) and two third pillars (8) are provided on the top of the middle plate (4). Two fixing plates (9) are provided above the middle plate (4). The two fixing plates (9) are fixed to the two second pillars (7) and the two third pillars (8) respectively. A top plate (10) is provided between the two fixing plates (9). A Nanopore third-generation sequencer (11), a Qubit fluorescence quantitative instrument (12) and a laptop micro workstation (13) are fixedly installed on the top plate (10).

2. The integrated device for in-situ microbial detection according to claim 1, characterized in that, The integrated device for in-situ detection of microorganisms also includes a protective cover (14), which is fixed to the first support (3) and the top plate (10) by multiple screws.

3. The integrated device for in-situ microbial detection according to claim 1, characterized in that, The base plate (1) is provided with a spiral groove (15), which is used to limit the position of the protective cover (14).

4. The integrated device for in-situ microbial detection according to claim 1, characterized in that, Both sides of the top plate (10) are fixed with rotating shafts, and the two rotating shafts are rotatably connected to the corresponding fixed plates (9).

5. The integrated device for in-situ detection of microorganisms according to claim 1, characterized in that, A connecting plate (16) is rotatably mounted on one side of the fixing plate (9), and the connecting plate (16) is fixed to the top plate (10) by screws.

6. The integrated device for in-situ detection of microorganisms according to claim 1, characterized in that, Two fixed rectangular rods (17) are fixedly installed at the bottom of the two second pillars (7), and two movable rectangular rods (20) are provided on the two third pillars (8). Two rectangular holes (18) are opened on the base plate (1), and the rectangular holes (18) are adapted to the fixed rectangular rods (17). Four rectangular slots (19) are opened on the top of the middle plate (4), and the fixed rectangular rods (17) and the movable rectangular rods (20) are adapted to the rectangular slots (19). When the two fixed rectangular rods (17) and the two movable rectangular rods (20) are respectively located in the corresponding rectangular slots (19), the whole device is in a highly superimposed state; when the fixed rectangular rods (17) are located in the two rectangular holes (18), the whole device is in a flat state.

7. The integrated device for in-situ detection of microorganisms according to claim 6, characterized in that, The bottom end of the third support column (8) is provided with a sliding groove (21), and the movable rectangular rod (20) is slidably installed inside the sliding groove (21). The two third supports columns (8) are provided with a limiting groove (22) on the side that is close to each other. The sliding groove (21) and the limiting groove (22) are connected. A lead screw (23) is fixedly installed on one side of the movable rectangular rod (20). The lead screw (23) passes through the limiting groove (22), and a nut (24) is threaded on the outside of the limiting groove (22).

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