A device for separating and purifying cosmetic functional materials using affinity chromatography

Abstract

The utility model discloses a kind of device for separating and purifying cosmetic functional raw materials using affinity chromatography technology, it is related to chromatography column field, including fixed plate, chromatography column, heat preservation shell and connecting assembly, chromatography column is by effluent bucket, glass shell and top cover composition, fixed plate fixedly sets in effluent bucket outer wall, effluent bucket and top cover are with glass shell and are formed sealed cavity by thread connection, heat preservation shell is clamped by two half shells and is wrapped in chromatography column outer wall, connecting assembly is installed in fixed plate lower surface, connecting assembly is used for the fixation and separation of two half shells, the utility model is split by heat preservation shell, so that scale can be completely removed in cleaning process, improve heat transfer efficiency, ensure the uniformity of chromatography column internal temperature distribution, conducive to maintaining the stability and separation effect of chromatographic process.

Description

Technical Field

[0001] This utility model mainly relates to the technical field of chromatography columns, specifically a device for separating and purifying cosmetic functional raw materials using affinity chromatography technology. Background Technology

[0002] When separating and purifying cosmetic active ingredients, the separation and purification device uses affinity chromatography technology, which includes a control unit, a first pump unit, a second pump unit, and a separation chromatography unit. Separation is achieved by utilizing the specific interactions between molecules, thereby improving separation efficiency and purity. This method is suitable for the fine separation of various cosmetic ingredients. The separation chromatography unit is used for the separation and extraction of cosmetic active ingredients.

[0003] A separation and extraction chromatography column described in the prior art includes a lower fixed plate and an upper fixed plate, with the chromatography column pressed between the lower fixed plate and the upper fixed plate. A funnel is threaded to the lower end of the lower fixed plate, and a sealing cap is fixedly installed on the upper end of the upper fixed plate. An insulation shell is fixedly installed on the outside of the chromatography column, and a screw blade is threaded to the inside of the insulation shell.

[0004] While the above technologies can facilitate temperature adjustment inside the chromatography column, heat preservation, and cleaning of the packing material inside the column, the insulation shell is fixed to the chromatography column. When the hardness of the flowing water is higher, scale is more likely to form. Over time, scale can adhere to the screw blades and the inner wall of the insulation shell, affecting temperature distribution and heat transfer efficiency, and making internal cleaning or replacement inconvenient. Utility Model Content

[0005] Based on this, the purpose of this utility model is to provide an apparatus for separating and purifying cosmetic active ingredients using affinity chromatography technology, so as to solve the technical problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] An apparatus for separating and purifying cosmetic active ingredients using affinity chromatography technology includes a fixed plate, a chromatography column, an insulating shell, and a connecting assembly. The chromatography column consists of an outlet hopper, a glass shell, and a top cover. The fixed plate is fixedly sleeved on the outer wall of the outlet hopper. The outlet hopper and the top cover are both threadedly connected to the glass shell to form a sealed cavity. The insulating shell is secured and wrapped around the outer wall of the chromatography column by two half-shells. The connecting assembly is installed on the lower surface of the fixed plate and is used for fixing and separating the two half-shells.

[0008] Specifically, in this technical solution, the connecting components include two protective shells and clamps. The two protective shells are located on both sides of the lower surface of the fixing plate. Both protective shells are convex in shape, and a bidirectional motor is installed in the center of the interior of each of the two protective shells by screws. The two output ends of the two bidirectional motors are connected to the shafts through flanges, and a drive gear is fixedly sleeved on the outer wall of the end of each shaft.

[0009] Specifically, the fixed plate has symmetrical movable slots on both sides, and a movable block is slidably installed in each movable slot. The lower surface of each movable block is provided with inner teeth. The upper tooth surface of each drive gear extends through the protective shell to the outside and meshes with the inner teeth. An arc-shaped magnetic suction plate is fixed on the side of the upper surface of each movable block near the chromatography column. Iron plates are symmetrically embedded in the bottom of the outer walls of the two half-shells. The arc-shaped magnetic suction plate is magnetically attracted to the iron plate.

[0010] Specifically, in this technical solution, support plates are fixed on both sides of the lower surface of the fixed plate, which are located on both sides of the liquid outlet hopper, and both protective shells are fixed to the outer wall of the support plate by screws through support rods.

[0011] Specifically, in this technical solution, the outer walls of the liquid outlet hopper and the top cover are both fixed with annular clamping plates, and the top and bottom of the inner walls of the two half-shells are provided with grooves that match the annular clamping plates. The contact parts of the two half-shells are bonded with sealing strips, and the clamps are fitted onto the top of the outer walls of the two half-shells with screws.

[0012] Specifically, the bottom of the liquid outlet hopper is connected to a liquid outlet pipe, a baffle is attached inside the liquid outlet hopper, a vertical rod is fixed at the center of the upper surface of the baffle, the top outer wall of the vertical rod is threaded, a plunger is fitted on the outer wall of the vertical rod, a sleeve is fixedly inserted at the center of the plunger, the sleeve is connected to the vertical rod by threads, and the upper surface of the plunger is set as an arc surface.

[0013] Specifically, in this technical solution, a liquid inlet pipe is provided in the center of the top cover, and the bottom end of the liquid inlet pipe is connected to a telescopic hose. The bottom end of the telescopic hose is 5cm away from the upper surface of the plunger to maintain a constant distance and prevent liquid splashing. The bottom outer wall of the telescopic hose and the outer wall of the sleeve are movably fitted with annular sleeve plates, and the two annular sleeve plates are fixedly connected by a connecting plate.

[0014] In summary, the present invention has the following advantages: the disassembly of the heat insulation shell allows for thorough removal of scale during the cleaning process, improves heat transfer efficiency, ensures uniform temperature distribution inside the chromatography column, and helps maintain the stability and separation effect of the chromatography process.

[0015] Furthermore, by regularly cleaning and removing scale, the corrosion and damage of scale to the outer wall of the chromatography column components are reduced, the risk of equipment failure caused by scale adhesion is lowered, and the service life of the chromatography column and related components is extended. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall positive axis structure of the chromatography column of this utility model;

[0017] Figure 2 This is a schematic diagram of the disassembled front axial side structure of the thermal insulation shell of this utility model;

[0018] Figure 3 This is a schematic diagram of the split-axis structure of the chromatography column of this utility model;

[0019] Figure 4 This utility model Figure 3 A schematic diagram of the oblique axis side structure;

[0020] Figure 5 This utility model Figure 3 Front view structural diagram;

[0021] Figure 6 This utility model Figure 2 Enlarged view of point A in the middle.

[0022] Figure Descriptions: 1. Fixed plate; 101. Support plate; 102. Moving channel; 2. Chromatography column; 201. Liquid outlet hopper; 2011. Liquid outlet pipe; 202. Glass shell; 203. Top cover; 2031. Liquid inlet pipe; 2032. Telescopic hose; 204. Annular retaining plate; 205. Baffle; 206. Vertical rod; 207. Plunger; 2071. Sleeve; 3. Annular sleeve plate; 301. Connecting plate; 4. Insulation shell; 401. Half shell; 4011. Slot; 5. Connecting assembly; 501. Protective shell; 502. Bidirectional motor; 503. Shaft; 504. Drive gear; 505. Moving block; 5051. Internal gear; 506. Arc-shaped magnetic suction plate; 507. Clamp. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0024] The embodiments of this utility model will be described below based on its overall structure.

[0025] In this embodiment, please refer to Figures 1-5As shown, an apparatus for separating and purifying cosmetic active ingredients using affinity chromatography technology includes a fixed plate 1, a chromatography column 2, a heat-insulating shell 4, and a connecting assembly 5. The chromatography column 2 consists of an outlet hopper 201, a glass shell 202, and a top cover 203. The fixed plate 1 is fixedly sleeved on the outer wall of the outlet hopper 201. The outlet hopper 201 and the top cover 203 are both connected to the glass shell 202 by threads to form a sealed cavity. The heat-insulating shell 4 is clamped and wrapped around the outer wall of the chromatography column 2 by two half-shells 401. The connecting assembly 5 is installed on the lower surface of the fixed plate 1. The connecting assembly 5 is used for fixing and separating the two half-shells 401. The outlet hopper 201 and the top cover 203 are both made of 316L stainless steel, which is pressure-resistant, corrosion-resistant, and easy to clean.

[0026] The bottom of the liquid outlet hopper 201 is connected to the liquid outlet pipe 2011. A baffle 205 is attached inside the liquid outlet hopper 201. A vertical rod 206 is fixed at the center of the upper surface of the baffle 205. The top outer wall of the vertical rod 206 is threaded. A plunger 207 is fitted on the outer wall of the vertical rod 206. A sleeve 2071 is fixedly inserted through the center of the plunger 207. The sleeve 2071 is connected to the vertical rod 206 by threads. The upper surface of the plunger 207 is set as an arc surface. A liquid inlet pipe 2031 is inserted through the center of the top cover 203. The bottom end of the liquid inlet pipe 2031 is connected to a telescopic hose 2032. The bottom end of the telescopic hose 2032 is 5cm away from the upper surface of the plunger 207. Both the bottom outer wall of the telescopic hose 2032 and the outer wall of the sleeve 2071 are movably fitted with annular sleeve plates 3. The two annular sleeve plates 3 are fixedly connected by a connecting plate 301.

[0027] Rotating the sleeve 2071 drives the plunger 207 to move up and down along the vertical rod 206, ensuring that the loading between the lower surface of the plunger 207 and the baffle 205 is optimal. The appropriate chromatography packing is carefully loaded between the plunger 207 and the baffle 205, making full preparation for the subsequent separation process. During the movement of the sleeve 2071, the telescopic hose 2032 is also adjusted synchronously through the annular sleeve 3 and the connecting plate 301 to ensure that the distance between the liquid inlet pipe 2031 and the upper surface of the plunger 207 is constant, avoiding liquid splashing due to the distance.

[0028] Then, the two half-shells 401 are placed on both sides of the outer wall of the chromatography column 2 and aligned with the arc-shaped magnetic suction plate 506 of the connecting component 5. The connecting component 5 is activated to make the two half-shells 401 fit tightly together to form a complete insulation shell 4. Then, the clamp 507 is fixed to the top of the insulation shell 4 to complete the assembly of the insulation shell 4. The top and bottom walls of the outer walls of the two half-shells 401 are respectively connected to the water inlet pipe and the water outlet pipe. The water inlet pipe is connected to the hot water source, and the water outlet pipe discharges the cooling water to form a closed temperature control system.

[0029] Next, depending on the amount of cosmetic functional raw material to be purified and the selected separation method, determine whether to use the first pump unit or the second pump unit. Turn on the selected pump unit and deliver the crude solution containing cosmetic functional raw material to the chromatography column 2 at a stable flow rate. During the delivery process, the mixer in the pump unit will ensure that the sample and buffer are fully and evenly mixed to ensure that the sample can be evenly contacted with the chromatography packing material and improve the separation efficiency.

[0030] When the sample solution enters the chromatography column 2 through the inlet pipe 2031 and the telescopic hose 2032, it flows down along the arc surface of the upper surface of the plunger 207 and comes into contact with the chromatography packing material. The target active ingredient will specifically bind to the specific binding sites on the chromatography packing material, while other impurities, due to the lack of such specific interaction with the packing material, cannot be fixed on the packing material and will flow out through the baffle 205 from the outlet pipe 2011. At this time, hot water from the hot water source enters the heat preservation shell 4 through the water inlet pipe and circulates in the heat preservation shell 4 to maintain a constant temperature, so as to optimize the separation conditions and further improve the separation effect and the recovery rate of the target component.

[0031] After the target component is separated, it needs to be eluted from the chromatographic packing for collection. At this time, the buffer solution is sequentially delivered into the chromatographic column 2 through the pump unit. Different buffer solutions are used to break the binding force between the target component and the packing by utilizing their different pH, ionic strength and other properties, so that the target component is detached from the packing and dissolved in the eluent and flows out of the chromatographic column 2.

[0032] By disassembling the insulation shell 4, scale can be thoroughly removed during the cleaning process, improving heat transfer efficiency and ensuring the uniformity of temperature distribution inside the chromatography column 2. This helps maintain the stability and separation effect of the chromatography process. Regular cleaning and removal of scale reduces corrosion and damage to the outer wall of the chromatography column 2 components, lowers the risk of equipment failure due to scale adhesion, and extends the service life of the chromatography column 2 and related components.

[0033] Please see Figures 2-6As shown, each connecting component 5 includes two protective shells 501 and a clamp 507. The two protective shells 501 are located on both sides of the lower surface of the fixing plate 1. Both protective shells 501 are convex in shape, and a bidirectional motor 502 is installed in the center of each protective shell 501 by screws. The two output ends of the two bidirectional motors 502 are connected to shafts 503 by flanges. A drive gear 504 is fixedly sleeved on the outer wall of the end of each shaft 503. The fixing plate 1 has symmetrical openings on both sides. Each movable channel 102 has a movable block 505 slidably installed in it. Each movable block 505 has an inner tooth 5051 on its lower surface. The upper tooth surface of each drive gear 504 extends through the protective shell 501 to the outside and meshes with the inner tooth 5051. An arc-shaped magnetic suction plate 506 is fixed on the side of the upper surface of each movable block 505 near the chromatography column 2. Iron plates are symmetrically embedded in the bottom of the outer walls of the two half-shells 401. The arc-shaped magnetic suction plate 506 is magnetically attracted to the iron plate.

[0034] Support plates 101 are fixed on both sides of the lower surface of the fixed plate 1, located on both sides of the liquid outlet 201. Both protective shells 501 are fixed to the outer wall of the support plate 101 by screws through support rods. Annular clamping plates 204 are fixed to the outer walls of the liquid outlet 201 and the top cover 203. The top and bottom of the inner walls of the two half-shells 401 are provided with grooves 4011 that match the annular clamping plates 204. Sealing strips are glued to the contact parts of the two half-shells 401. The clamps 507 are screwed onto the top of the outer walls of the two half-shells 401. The bidirectional motor 502 is controlled by an external control system.

[0035] Two half-shells 401 are placed on the fixed plate 1 and located on both sides of the chromatography column 2, so that the arc-shaped magnetic suction plate 506 is tightly attracted to the iron plate. Then, the bidirectional motor 502 is started by the control system. The output end of the bidirectional motor 502 drives the shaft 503 to rotate. The shaft 503 drives the drive gear 504 to rotate. The rotation of the drive gear 504 controls the inner tooth 5051 to move laterally, so that the moving block 505 slides along the moving through groove 102 and drives the arc-shaped magnetic suction plate 506 to move synchronously. The moving arc-shaped magnetic suction plate 506 pushes the attracted half-shells 401 to move until the two half-shells 401 are completely attached and the slot 4011 is precisely connected with the annular plate 204. The sealing strip ensures the sealing effect. Then, the clamp 507 is fastened to the top of the two half-shells 401 to complete the assembly of the heat insulation shell 4. This realizes the automated operation of opening and closing the heat insulation shell 4, which is convenient and quick, and can be replaced or cleaned regularly to avoid scale accumulation affecting heat transfer efficiency.

[0036] The working principle of this utility model is as follows:

[0037] Rotating the sleeve 2071 drives the plunger 207 to move up and down along the vertical rod 206, ensuring that the loading between the lower surface of the plunger 207 and the baffle 205 is optimal. The appropriate chromatography packing is carefully loaded between the plunger 207 and the baffle 205, making full preparation for the subsequent separation process. During the movement of the sleeve 2071, the telescopic hose 2032 is also adjusted synchronously through the annular sleeve 3 and the connecting plate 301 to ensure that the distance between the liquid inlet pipe 2031 and the upper surface of the plunger 207 is constant, avoiding liquid splashing due to the distance.

[0038] Then, the two half-shells 401 are placed on the fixed plate 1 and located on both sides of the chromatography column 2, so that the arc-shaped magnetic suction plate 506 is tightly attracted to the iron plate. Then, the bidirectional motor 502 is started by the control system. The output end of the bidirectional motor 502 drives the shaft 503 to rotate. The shaft 503 drives the drive gear 504 to rotate. The rotation of the drive gear 504 controls the inner tooth 5051 to move laterally, so that the moving block 505 slides along the moving through groove 102 and drives the arc-shaped magnetic suction plate 506 to move synchronously. The moving arc-shaped magnetic suction plate 506 pushes the attracted half-shells 401 to move until the two half-shells 401 are completely attached and the slot 4011 is precisely connected with the annular plate 204. The sealing strip ensures the sealing effect. Then, the clamp 507 is fastened to the top of the two half-shells 401 to complete the assembly of the insulation shell 4.

[0039] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. An apparatus for separating and purifying cosmetic active ingredients using affinity chromatography, comprising a fixed plate (1), a chromatography column (2), a heat-insulating shell (4), and a connecting assembly (5), characterized in that, The chromatography column (2) consists of an outlet hopper (201), a glass shell (202), and a top cover (203). The fixing plate (1) is fixedly sleeved on the outer wall of the outlet hopper (201). The outlet hopper (201) and the top cover (203) are both connected to the glass shell (202) by threads to form a sealed cavity. The heat insulation shell (4) is clamped and wrapped around the outer wall of the chromatography column (2) by two half shells (401). The connecting component (5) is installed on the lower surface of the fixing plate (1). The connecting component (5) is used for fixing and separating the two half shells (401).

2. The apparatus for separating and purifying cosmetic active ingredients using affinity chromatography according to claim 1, characterized in that, Each of the connecting components (5) includes two protective shells (501) and a clamp (507). The two protective shells (501) are located on both sides of the lower surface of the fixing plate (1). The two protective shells (501) are convex in shape. A bidirectional motor (502) is installed in the center of the interior of each of the two protective shells (501) by screws. The two output ends of the two bidirectional motors (502) are connected to the shafts (503) through flanges. A drive gear (504) is fixedly sleeved on the outer wall of the end of each shaft (503).

3. The apparatus for separating and purifying cosmetic active ingredients using affinity chromatography according to claim 2, characterized in that, The fixed plate (1) has symmetrical movable slots (102) on both sides. Each movable slot (102) has a movable block (505) slidably installed in it. Each movable block (505) has an inner tooth (5051) on its lower surface. The upper tooth surface of each drive gear (504) extends through the protective shell (501) to the outside and meshes with the inner tooth (5051). Each movable block (505) has an arc-shaped magnetic suction plate (506) fixed on the side of its upper surface near the chromatography column (2). The bottom of the outer walls of the two half-shells (401) are symmetrically embedded with iron plates. The arc-shaped magnetic suction plate (506) is magnetically attracted to the iron plate.

4. The apparatus for separating and purifying cosmetic active ingredients using affinity chromatography according to claim 2, characterized in that, The lower surface of the fixed plate (1) is fixed with support plates (101) on both sides of the liquid outlet hopper (201), and the two protective shells (501) are fixed to the outer wall of the support plate (101) by support rods and screws.

5. The apparatus for separating and purifying cosmetic active ingredients using affinity chromatography according to claim 2, characterized in that, The outer walls of the liquid outlet (201) and the top cover (203) are both fixed with annular clamping plates (204). The top and bottom of the inner walls of the two half-shells (401) are provided with clamping grooves (4011) that match the annular clamping plates (204). The contact parts of the two half-shells (401) are bonded with sealing strips. The clamps (507) are screwed onto the top of the outer walls of the two half-shells (401).

6. The apparatus for separating and purifying cosmetic functional raw materials using affinity chromatography according to claim 1, characterized in that, The bottom of the liquid outlet hopper (201) is connected to the liquid outlet pipe (2011). A baffle (205) is attached inside the liquid outlet hopper (201). A vertical rod (206) is fixed at the center of the upper surface of the baffle (205). The top outer wall of the vertical rod (206) is threaded. A plunger (207) is fitted on the outer wall of the vertical rod (206). A sleeve (2071) is fixedly inserted at the center of the plunger (207). The sleeve (2071) is connected to the vertical rod (206) by threads. The upper surface of the plunger (207) is set as an arc surface.

7. The apparatus for separating and purifying cosmetic functional raw materials using affinity chromatography according to claim 6, characterized in that, A liquid inlet pipe (2031) is provided in the center of the top cover (203). The bottom end of the liquid inlet pipe (2031) is connected to a telescopic hose (2032). The bottom end of the telescopic hose (2032) is 5cm away from the upper surface of the plunger (207). The bottom outer wall of the telescopic hose (2032) and the outer wall of the sleeve (2071) are movably fitted with annular sleeve plates (3). The two annular sleeve plates (3) are fixedly connected by a connecting plate (301).

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