A biological sample detection cartridge

Abstract

The utility model belongs to biological sample detection technical field, concretely relates to a biological sample detection box. The utility model provides a biological sample detection box, aims at solving the problem of big volume and low detection efficiency of biological sample detection equipment in prior art. The utility model provides a biological sample detection box, including the membrane type micro -fluidic chip integrated in the box body, the preheating assembly for preheating in the sample extraction process, the drive assembly for driving liquid flow in the membrane type micro -fluidic chip and the temperature control assembly for heating or cooling the sample after extraction in the membrane type micro -fluidic chip. Adopting the membrane type micro -fluidic chip, the membrane type micro -fluidic chip and preheating assembly and temperature control assembly have greater contact area, so as to improve the heat exchange efficiency, and further improve the sample extraction and detection efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of biological sample detection technology, and specifically relates to a biological sample detection kit. Background Technology

[0002] A PCR instrument is short for a PCR amplification instrument. It is an instrument that uses polymerase chain reaction technology to amplify a specific segment of DNA and is widely used in medical and biological laboratories.

[0003] The principle of PCR technology is similar to DNA replication, consisting of three basic reaction steps: denaturation, annealing, and extension. First, the double-stranded DNA is dissociated into single-stranded DNA at high temperature. After cooling, primers pair with the single-stranded template DNA. Finally, under the action of polymerase, a DNA strand complementary to the single-stranded template DNA is synthesized.

[0004] In existing technologies, rigid chips are typically used as amplification containers to carry samples. Rigid chips are large in size and have uneven heating, resulting in low amplification efficiency and large amplification equipment size. Utility Model Content

[0005] This invention provides a biological sample detection kit, which aims to solve the problems of large size and low detection efficiency of existing biological sample detection equipment.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0007] A biological sample detection kit includes a thin-film microfluidic chip, a temperature control component, a preheating component, and a drive component integrated within the kit body;

[0008] The temperature control component is used to heat or cool the biological sample extracted within the thin-film microfluidic chip after extraction.

[0009] The preheating component is used to preheat the liquid inside the thin-film microfluidic chip during the extraction of biological samples;

[0010] The driving component is used to drive the liquid in the thin-film microfluidic chip to flow in the thin-film microfluidic chip according to the biological sample extraction process.

[0011] A further improved solution: The thin-film microfluidic chip includes at least two cavities containing liquid, the at least two cavities being connected by a flow channel, and the driving assembly includes a driving unit for driving the liquid in the thin-film microfluidic chip to flow within the thin-film microfluidic chip according to a biological sample extraction process, and a control unit for controlling the opening and closing of the flow channel.

[0012] Based on the above technical solution: the driving component includes a driving unit and a control unit. The driving unit is used to drive the liquid to flow inside the thin-film microfluidic chip according to the extraction operation. The control unit is used to control the opening and closing of the flow channel to prevent the liquid from flowing arbitrarily inside the thin-film microfluidic chip, thereby improving the sample extraction accuracy of the thin-film microfluidic chip.

[0013] A further improved solution: The driving unit includes a first electromagnet, which presses the thin-film microfluidic chip corresponding to the cavity through a pressure plate, and the cavity corresponds one-to-one with the driving unit.

[0014] Based on the above technical solution: the driving unit includes a first electromagnet, which is squeezed by a pressure plate. The pressure plate and the thin-film microfluidic chip have a larger contact area, so that the liquid in the cavity can be completely squeezed out, preventing residual liquid in the cavity.

[0015] A further improved solution: The control unit includes a second electromagnet, which uses a pressure head to press the thin-film microfluidic chip corresponding to the flow channel.

[0016] Based on the above technical solution: the control unit includes a second electromagnet, which presses the thin-film microfluidic chip corresponding to the flow channel through a pressure head. The pressure head and the thin-film microfluidic chip have a larger contact area, which can effectively disconnect the flow channel and prevent liquid from passing through the flow channel.

[0017] A further improved solution: The control unit further includes a support base for supporting the thin-film microfluidic chip, and the support base is provided with a groove corresponding to the pressure head, and the pressure head presses a portion of the thin-film microfluidic chip into the groove.

[0018] Based on the above technical solution: the control unit also includes a support base for supporting the thin-film microfluidic chip. The support base is provided with a groove corresponding to the pressure head. The pressure head can squeeze the part of the thin-film microfluidic chip corresponding to the flow channel into the groove, causing the thin-film microfluidic chip to bend, thereby better disconnecting the flow channel and preventing liquid from passing through the flow channel.

[0019] A further improved solution: the cross-sectional shape of the groove is V-shaped; or, the cross-sectional shape of the groove is U-shaped.

[0020] Based on the above technical solution: the cross-sectional shape of the groove is V-shaped, or the cross-sectional shape of the groove is U-shaped. There are multiple options for the shape of the groove, which reduces the manufacturing cost of the support.

[0021] A further improved scheme: The cavity includes a pre-storage cavity, a lysis cavity, a washing cavity, an extraction cavity, an elution cavity, and an amplification cavity. The pre-storage cavity, lysis cavity, washing cavity, elution cavity, and amplification cavity are all connected to the extraction cavity through independent flow channels, and each flow channel corresponds to the control unit.

[0022] Based on the above technical solution: the cavity includes a pre-storage cavity, a lysis cavity, a cleaning cavity, an extraction cavity, an elution cavity, and an amplification cavity. The sample can be extracted and amplified within a thin-film microfluidic chip, making the sample less susceptible to contamination and improving detection accuracy.

[0023] A further improved solution: the preheating component contacts the thin-film microfluidic chip corresponding to the extraction cavity.

[0024] Based on the above technical solution: the preheating component contacts the thin-film microfluidic chip corresponding to the extraction chamber, the preheating component can directly preheat the liquid in the extraction chamber, and the liquid in the extraction chamber is heated evenly.

[0025] A further improved solution: the temperature control component contacts the thin-film microfluidic chip corresponding to the amplification cavity.

[0026] Based on the above technical solution: the temperature control component is in contact with the thin-film microfluidic chip at the corresponding amplification cavity, and the temperature control component directly heats and cools the liquid in the amplification cavity. The liquid in the amplification cavity can quickly complete the heating and cooling cycle, which improves the amplification efficiency and the detection efficiency.

[0027] A further improved solution: The temperature control component includes a heater in contact with the thin-film microfluidic chip and a heat sink disposed on the heater.

[0028] Based on the above technical solution: the temperature control component includes a heater in contact with the thin-film microfluidic chip and a heat sink disposed on the heater. The temperature control component can quickly switch between heating and cooling modes, thereby improving detection efficiency.

[0029] The beneficial effects of this utility model are as follows:

[0030] This invention provides a biological sample detection kit, comprising a thin-film microfluidic chip integrated within the kit body, a preheating component for preheating during sample extraction, a driving component for driving liquid flow within the thin-film microfluidic chip, and a temperature control component for heating or cooling the sample after extraction within the thin-film microfluidic chip. The use of a thin-film microfluidic chip allows for a larger contact area between the chip and the preheating and temperature control components, thereby improving heat exchange efficiency and consequently enhancing sample extraction and detection efficiency.

[0031] The use of thin-film microfluidic chips, which are small in size, allows for a smaller biological sample detection kit, making it easy to carry.

[0032] The use of thin-film microfluidic chips, with their thinner walls, improves thermal conductivity, further enhancing sample extraction and detection efficiency. Attached Figure Description

[0033] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For users of ordinary skills in the art, other related drawings can be obtained from these drawings without creative effort.

[0034] Figure 1 This is a front view of a biological sample detection kit according to this utility model.

[0035] Figure 2 This is a schematic diagram of a biological sample detection kit according to the present invention.

[0036] Figure 3 This is a schematic diagram of the arrangement of the driving components in a biological sample detection kit according to this utility model.

[0037] Figure 4 This is a schematic diagram of the arrangement of the driving component relative to the thin-film microfluidic chip in a biological sample detection kit according to this utility model.

[0038] Figure 5 This is a front view of a thin-film microfluidic chip in a biological sample detection kit according to this utility model.

[0039] Figure 6 This is a schematic diagram of the control unit in a biological sample detection kit according to this utility model.

[0040] Explanation of the labels in the diagram:

[0041] 1-Thin-film microfluidic chip; 11-Pre-storage chamber; 12-Pyrolysis chamber; 13-Cleaning chamber; 14-Extraction chamber; 15-Eluting chamber; 16-Amplification chamber; 17-Waste liquid chamber; 2-Temperature control component; 21-Heater; 22-Heat sink; 3-Drive component; 31-Drive unit; 311-Pressure plate; 32-Control unit; 321-Pressure head; 322-Support base; 323-Groove. Detailed Implementation

[0042] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present utility model and are not intended to limit the present utility model. All other embodiments obtained by users of the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0043] refer to Figures 1 to 6 A biological sample detection kit includes a thin-film microfluidic chip 1, a temperature control component 2, a preheating component, and a drive component 3 integrated within the kit body;

[0044] The temperature control component 2 is used to heat or cool the biological sample extracted from the thin-film microfluidic chip 1 after extraction.

[0045] The preheating component is used to preheat the liquid inside the thin-film microfluidic chip 1 during the extraction of biological samples;

[0046] The driving component 3 is used to drive the liquid in the thin-film microfluidic chip 1 to flow in the thin-film microfluidic chip 1 according to the biological sample extraction process.

[0047] The preheating component is a structure that can generate heat, and can be a semiconductor heating block or similar structure.

[0048] Temperature control component 2 is used to output heat or cold to control the temperature rise or fall of the liquid in the thin-film microfluidic control chip in order to complete the amplification operation.

[0049] Temperature control component 2 can adopt a structure similar to a semiconductor heating element.

[0050] refer to Figures 1 to 6 The thin-film microfluidic chip 1 includes at least two cavities containing liquid, and the at least two cavities are connected by a flow channel. The driving component 3 includes a driving unit 31 that drives the liquid in the thin-film microfluidic chip 1 to flow in the thin-film microfluidic chip 1 according to the biological sample extraction process, and a control unit 32 that controls the opening and closing of the flow channel.

[0051] The thin-film microfluidic chip 1 includes a first thin film layer and a second thin film layer. The first thin film layer and the second thin film layer can be an integral membrane structure. The integral membrane structure is bent to form the first thin film layer and the second thin film layer. The first thin film layer and the second thin film layer are heat-sealed together, and the unsealed part forms a cavity and a flow channel.

[0052] A heat-sealing zone is formed between the first and second film layers at the heat-sealing area used to create the cavity and flow channel. A heat-sealing section can be formed within the flow channel to disconnect it. The heat-sealing strength of the heat-sealing section is lower than that of the heat-sealing zone, allowing the heat-sealing section to be opened by liquid flow, thus opening the flow channel. During sample extraction, a valve can be used to close the flow channel.

[0053] refer to Figures 1 to 6 The driving unit 31 includes a first electromagnet, which presses the thin-film microfluidic chip 1 corresponding to the cavity through a pressure plate 311. The cavity corresponds one-to-one with the driving unit 31.

[0054] There are multiple drive units 31, which are used to drive the flow of liquid in different cavities.

[0055] The control unit 32 includes a second electromagnet, which presses the thin-film microfluidic chip 1 corresponding to the flow channel through a pressure head 321.

[0056] The control unit 32 further includes a support base 322 for supporting the thin-film microfluidic chip 1. A groove 323 is provided on the support base 322 corresponding to the pressure head 321, and the pressure head 321 presses a portion of the thin-film microfluidic chip 1 into the groove 323. The control unit 32 has multiple functions for controlling the on / off states of different flow channels.

[0057] The cross-sectional shape of the groove 323 is V-shaped; or, the cross-sectional shape of the groove 323 is U-shaped.

[0058] refer to Figures 1 to 6 Specifically: the cavity includes a pre-storage cavity 11, a lysis cavity 12, a washing cavity 13, an extraction cavity 14, an elution cavity 15, and an amplification cavity 16. The pre-storage cavity 11, the lysis cavity 12, the washing cavity 13, the elution cavity 15, and the amplification cavity 16 are all connected to the extraction cavity 14 through independent flow channels, and the flow channels correspond one-to-one with the control unit 32.

[0059] The preheating component contacts the thin-film microfluidic chip 1 corresponding to the extraction chamber 14. The preheating component is typically used to preheat the liquid in the extraction chamber 14 to 56°C.

[0060] The temperature control component 2 is in contact with the thin-film microfluidic chip 1 corresponding to the amplification chamber 16. The temperature control component 2 is used to complete sample amplification, and is typically used to control the circulation of the sample between two temperatures.

[0061] The temperature control component 2 includes a heater 21 in contact with the thin-film microfluidic chip 1 and a heat sink 22 disposed on the heater 21. The heat sink 22 and the heater 21 can be an integral structure, or the heat sink 22 can be welded to the heater 21.

[0062] The working principle of this embodiment:

[0063] The thin-film microfluidic chip 1 may specifically include a pre-storage chamber 11, a lysis chamber 12, a cleaning chamber 13, an extraction chamber 14, an elution chamber 15, and an amplification chamber 16. The pre-storage chamber 11, the lysis chamber 12, the cleaning chamber 13, the elution chamber 15, and the amplification chamber 16 are all connected to the extraction chamber 14 through independent flow channels.

[0064] It may also include a waste liquid chamber 17 for storing waste liquid. The waste liquid chamber 17 is connected to the extraction chamber 14 through an independent flow channel. The flow channel connecting the waste liquid chamber 17 and the extraction chamber 14 also corresponds to an independent control unit 32.

[0065] The cleaning chamber 13 may include a second cleaning chamber 13 and a second cleaning chamber 13, the first cleaning chamber 13 and the second cleaning chamber 13 are connected in series and communicate with the extraction chamber 14.

[0066] The biological sample testing kit includes the following testing steps:

[0067] S10, the sample is injected into the pre-storage cavity in any way. Then, the drive unit 31 corresponding to the pre-storage cavity 11 works. At the same time, the control unit 32 corresponding to the flow channel connecting the pre-storage cavity 11 and the pyrolysis cavity 12 works to open the flow channel. The liquid in the pre-storage cavity 11 is squeezed into the pyrolysis cavity 12. In the pyrolysis cavity 12, the sample and reagent are pyrolyzed in a temperature range of 50°C to 60°C. The sample and reagent are pyrolyzed at a temperature of 50°C, 60°C or 56°C. A guide part can be set in the pyrolysis cavity 12. The guide part can be formed by heat sealing the first film layer and the second film layer. The liquid entering the pyrolysis cavity 12 circulates around the guide part for mixing and pyrolysis. During this process, all flow channels communicating with the pyrolysis cavity 12 are closed by the corresponding control unit 32.

[0068] S20, after the liquid in the pyrolysis chamber 12 is completely pyrolyzed, the drive unit 31 corresponding to the pyrolysis chamber 12 operates, controlling the control unit 32 of the flow channel between the pyrolysis chamber 12 and the extraction chamber 14 to open, squeezing the liquid in the pyrolysis chamber 12 into the extraction chamber 14, where the pyrolyzed sample is adsorbed. A silicon cavity membrane for adsorbing the sample can be set in the extraction chamber 14. During the process of the liquid in the pyrolysis chamber 12 entering the extraction chamber 14, all flow channels connecting the extraction chamber 14 are disconnected except those connected to the pyrolysis chamber 12.

[0069] S30: The driving unit 31 corresponding to the first cleaning chamber 13 operates. Simultaneously, the control unit 32 corresponding to the flow channel communicating between the first cleaning chamber 13 and the extraction chamber 14 opens. The liquid in the first cleaning chamber 13 is squeezed into the extraction chamber 14, using the cleaning liquid in the first cleaning chamber 13 to perform the first cleaning of the sample adsorbed in the extraction chamber 14. During this process, all flow channels communicating with the extraction chamber 14, except for the flow channel communicating with the first cleaning chamber 13, are disconnected. All of these are disconnected; the driving unit 31 corresponding to extraction chamber 14 can work repeatedly to squeeze the liquid in extraction chamber 14, so that the sample in extraction chamber 14 is repeatedly cleaned. During the cleaning process, the pressure plate 311 corresponding to extraction chamber 14 can work repeatedly within 1 / 2 stroke or less than 2 / 3 stroke to repeatedly squeeze extraction chamber 14 to complete the first cleaning of the sample. After the sample in extraction chamber 14 has completed the first cleaning, the driving unit 31 corresponding to extraction chamber 14 works, the channel connecting extraction chamber 14 to waste liquid chamber 17 is opened, and waste liquid enters waste liquid chamber 17. Alternatively, the driving unit 31 corresponding to extraction chamber 14 works, the channel connecting to pyrolysis chamber 12 is opened, and waste liquid enters pyrolysis chamber 12.

[0070] S40, referring to the cleaning process of the first cleaning chamber 13, the sample in the extraction chamber 14 after the first cleaning is cleaned a second time using the cleaning liquid in the second cleaning chamber 13. During the cleaning process, the preheating component is working, and the sample after the first cleaning is cleaned a second time at a temperature range of 60°C to 70°C, preferably at 65°C. The liquid in the second cleaning chamber 13 can enter the extraction chamber 14 through the first cleaning chamber 13. Alternatively, the second cleaning chamber 13 can also be an independent chamber. The second cleaning process of the sample in the extraction chamber 14 in the second cleaning chamber 13 refers to the first cleaning process. The waste liquid can still enter the waste liquid chamber 17 or the pyrolysis chamber 12.

[0071] S50, after the liquid in extraction chamber 14 has completed its second washing, the drive unit 31 corresponding to elution chamber 15 operates, squeezing the liquid in elution chamber 15 into extraction chamber 14. The sample after the second washing is then eluted using the eluent in elution chamber 15 at a temperature range of 60°C to 70°C to complete the extraction of the biological sample. Elution is preferably performed at 65°C. During this process, all flow channels communicating with extraction chamber 14, except those communicating with elution chamber 15, are disconnected.

[0072] S60, after the sample is extracted in the extraction chamber 14, the drive unit 31 corresponding to the extraction chamber 14 operates, disconnecting all channels connecting the extraction chamber 14 except those connecting to the amplification chamber 16, thus squeezing the sample from the extraction chamber 14 into the amplification chamber 16. Then, the channel connecting to the amplification chamber 16 is closed by the corresponding control unit 32, and the temperature control component 2 operates to perform amplification on the sample in the amplification chamber 16.

[0073] Finally, the biological sample detection kit may also include an optical system for optical detection of the amplified sample.

[0074] This utility model is not limited to the above-mentioned optional embodiments. Under the premise of non-contradiction, the various solutions can be combined arbitrarily. Anyone can derive other forms of products under the guidance of this utility model. However, no matter what changes are made in their shape or structure, all technical solutions that fall within the scope of the claims of this utility model are within the protection scope of this utility model.

Claims

1. A biological sample detection kit, characterized in that: This includes a thin-film microfluidic chip, a temperature control component, a preheating component, and a drive component integrated within the housing; The temperature control component is used to heat or cool the biological sample extracted within the thin-film microfluidic chip after extraction. The preheating component is used to preheat the liquid inside the thin-film microfluidic chip during the extraction of biological samples; The driving component is used to drive the liquid in the thin-film microfluidic chip to flow in the thin-film microfluidic chip according to the biological sample extraction process.

2. The biological sample detection kit according to claim 1, characterized in that: The thin-film microfluidic chip includes at least two cavities containing liquid, which are connected by a flow channel. The driving assembly includes a driving unit that drives the liquid in the thin-film microfluidic chip to flow within the chip according to a biological sample extraction process, and a control unit that controls the opening and closing of the flow channel.

3. A biological sample detection kit according to claim 2, characterized in that: The driving unit includes a first electromagnet, which presses the thin-film microfluidic chip corresponding to the cavity through a pressure plate. The cavity corresponds one-to-one with the driving unit.

4. A biological sample detection kit according to claim 2, characterized in that: The control unit includes a second electromagnet, which presses the thin-film microfluidic chip corresponding to the flow channel through a pressure head.

5. A biological sample detection kit according to claim 4, characterized in that: The control unit also includes a support base for supporting the thin-film microfluidic chip. The support base has a groove corresponding to the pressure head, and the pressure head presses a portion of the thin-film microfluidic chip into the groove.

6. A biological sample detection kit according to claim 5, characterized in that: The groove has a V-shaped cross-section; or, the groove has a U-shaped cross-section.

7. A biological sample detection kit according to claim 2, characterized in that: The cavity includes a pre-storage cavity, a lysis cavity, a washing cavity, an extraction cavity, an elution cavity, and an amplification cavity. The pre-storage cavity, lysis cavity, washing cavity, elution cavity, and amplification cavity are all connected to the extraction cavity through independent flow channels, and each flow channel corresponds to the control unit.

8. A biological sample detection kit according to claim 7, characterized in that: The preheating component comes into contact with the thin-film microfluidic chip corresponding to the extraction cavity.

9. A biological sample detection kit according to claim 7, characterized in that: The temperature control component is in contact with the thin-film microfluidic chip corresponding to the amplification cavity.

10. A biological sample detection kit according to claim 9, characterized in that: The temperature control component includes a heater in contact with the thin-film microfluidic chip and a heat sink disposed on the heater.

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