A high performance liquid chromatography tandem mass spectrometry all-in-one machine

Abstract

The application relates to the technical field of liquid chromatography tandem mass spectrometry all-in-one machines, in particular to a high-performance liquid chromatography tandem mass spectrometry all-in-one machine which comprises a bottle box, the top of the bottle box is used for placing a solvent bottle, a fixing clamping plate for limiting the solvent bottle is arranged on the bottle box, a side edge buckle slot is formed in the side edge of the bottle box, a refrigeration box is arranged in the side edge cooling box, an intelligent control screen is mounted on the surface of the refrigeration box, a pull-out box is connected to the side edge cooling box through a pull-out slot, and a box handle is arranged on the pull-out box. On the sample management, the refrigeration box in the side edge cooling box cooperates with the intelligent control screen to realize intelligent temperature control, the influence of manual operation on the refrigeration environment is avoided, the arc-shaped clamping groove, the rubber pad and the compression spring structure in the pull-out box can stably clamp a test tube, the sample pollution risk is reduced, the detection precision is improved, and meanwhile, the detachable connection design of the fixing clamping plate enhances the use flexibility of the equipment.

Description

Technical Field

[0001] This application relates to the field of integrated liquid chromatography-tandem mass spectrometry, and in particular to a high-performance liquid chromatography-tandem mass spectrometry integrated system. Background Technology

[0002] The integrated liquid chromatography-tandem mass spectrometry system is used for the qualitative and quantitative detection of pesticide residues, veterinary drug residues, illegal additives, trace pollutants and other trace (including ultra-trace) components in samples such as food, feed, plant-derived products and their products, food packaging, animal-derived products and their products, and water samples. The instrument type is a tandem quadrupole mass spectrometer, which should meet the requirements of high-throughput quantitative analysis and qualitative analysis functions of liquid chromatography-mass spectrometry.

[0003] A search revealed that Chinese Patent Publication No. CN218412396U discloses a liquid chromatography-tandem mass spectrometry (LC-MS / MS) detector for detecting olanzapine concentration in serum. The detector includes a main body, a connecting plate fixedly connected to the side wall of the main body, a placement box fixedly connected to the side wall of the connecting plate, a cover plate hinged to the upper side of the placement box, a cooler provided on the bottom side wall of the placement box, a support mesh fixedly connected to the inner side wall of the placement box, and a support plate fixedly connected to the inner side wall of the placement box.

[0004] Regarding the aforementioned technologies, the inventors have discovered the following drawbacks: While a suitable refrigeration storage device is used to assist in accommodating the samples to be tested, manual sample introduction requires opening the device when taking or removing serum samples during the overall testing process. This manual sample introduction process has many drawbacks. On the one hand, opening the refrigeration device can easily affect the internal temperature, impacting the refrigeration storage effect. On the other hand, manual operation can easily lead to contamination of the serum samples, thus affecting the detection accuracy. Furthermore, existing liquid chromatography-tandem mass spectrometry (LC-MS / MS) instruments often occupy a large space in their layout, with insufficiently compact connections between components, resulting in complex piping connections, low signal transmission efficiency, and affecting the overall operating efficiency of the equipment. However, this patented technology does not provide a solution to this problem. Utility Model Content

[0005] To address the problems mentioned in the background section, this application provides a high-performance liquid chromatography-tandem mass spectrometry integrated system.

[0006] This application provides a high-performance liquid chromatography-tandem mass spectrometry integrated system, which adopts the following technical solution: It includes a bottle box, the top of which is used to place solvent bottles, a fixing plate for limiting the solvent bottles on the bottle box, a side handle groove on the side of the bottle box, and a vacuum degasser, a quaternary pump, an autosampler, a column oven, and a detector connected sequentially below the bottle box; side cooling boxes are connected to the right side of the vacuum degasser, the right side of the quaternary pump, and the right side of the autosampler, and a cooling box is provided inside the side cooling box. An intelligent control screen is installed on the surface of the cooling box, and a pull-out box is connected to the side cooling box via a pull-out groove, with a handle on the pull-out box.

[0007] Optionally, the bottom of the fixing plate is provided with a positioning hexagonal post, and the top of the bottle box is provided with a hexagonal groove and a lower positioning groove that are adapted to the positioning hexagonal post. The fixing plate can be detachably connected to the bottle box by the positioning hexagonal post, the hexagonal groove and the lower positioning groove cooperating with each other.

[0008] Optionally, the pull-out box is provided with a first clamping plate, a middle plate, a second clamping plate, and a compression spring. The first clamping plate and the second clamping plate are provided with clamping grooves, and rubber pads are provided in the clamping grooves. The compression spring is fitted with a limiting sleeve. One end of the compression spring is connected to the second clamping plate and the other end is connected to the left and right sides of the middle plate. The middle plate is provided with a label.

[0009] Optionally, the contact area between the fixing plate and the bottle box is provided with a resistance layer to increase the stability of the fixing plate when installed on the bottle box.

[0010] Optionally, the vacuum degasser, quaternary pump, autosampler, column oven, and detector are stacked vertically in sequence.

[0011] Optionally, the refrigeration box is embedded in the internal space of the side cooling box, the intelligent control screen is embedded and fitted with the outer surface of the refrigeration box, and the intelligent control screen is connected to the internal control module of the device through a circuit.

[0012] Optionally, the No. 1 clamping plate and the No. 2 clamping plate have the same structure and are symmetrically distributed from left to right. The multiple clamping slots are all arc-shaped and are arranged at equal intervals along the length of the No. 1 clamping plate and the length of the No. 2 clamping plate.

[0013] In summary, this application includes the following beneficial technical effects:

[0014] This invention achieves intelligent temperature control in sample storage and processing by using a cooling box inside the side cooling box in conjunction with an intelligent control screen. This avoids the impact of frequent manual opening of the refrigeration device on the temperature. At the same time, the pull-out box adopts structures such as arc-shaped clamping grooves, rubber pads, compression springs and limiting cylinders to securely store test tubes, reduce the risk of sample contamination during manual handling and improve detection accuracy.

[0015] This utility model improves the overall operating efficiency of the equipment by vertically stacking components such as the vacuum degasser and quaternary pump under the bottle box in terms of equipment layout and operating efficiency. This saves floor space, makes the structure compact, facilitates pipeline connection and signal transmission between components, and enhances the overall operating efficiency of the equipment. The detachable connection design between the fixing plate and the bottle box also enhances the flexibility of equipment use. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of an embodiment of this application;

[0017] Figure 2 This is a schematic diagram of the combined connection structure of the bottle box and the fixing plate in an embodiment of this application;

[0018] Figure 3 This is a schematic diagram of the disassembled structure of the side cooling box and the pull-out box in an embodiment of this application;

[0019] Figure 4 This is a schematic diagram of the disassembled structure of the pull-out box, clamping plate number one, clamping plate number two, and compression spring in the embodiments of this application.

[0020] Reference numerals: 1. Solvent bottle; 2. Bottle box; 3. Fixing plate; 4. Side handle groove; 5. Quaternary pump; 6. Autosampler; 7. Column oven; 8. Detector; 9. Side cooling box; 10. Cooling box; 11. Intelligent control panel; 12. Pull-out box; 13. Box handle; 14. Lower positioning groove; 15. Positioning hexagonal column; 16. Hexagonal groove; 17. Pull-out groove; 18. No. 1 clamping plate; 19. Clamping groove; 20. Rubber pad; 21. Intermediate plate; 22. Label; 23. No. 2 clamping plate; 24. Compression spring; 25. Limiting cylinder; 26. Resistance layer; 27. Vacuum degasser. Detailed Implementation

[0021] The following is in conjunction with the appendix Figure 1-4 This application will be described in further detail.

[0022] This application discloses a high-performance liquid chromatography-tandem mass spectrometry integrated system. For example... Figure 1 and Figure 2As shown, the apparatus includes a bottle box 2. The top of the bottle box 2 is used to place solvent bottles 1, providing a stable storage space for the solvent bottles 1, facilitating the use of solvents by experimental personnel, and ensuring the stability of solvent supply. The top of the bottle box 2 is equipped with a fixing plate 3 for limiting the position of the solvent bottles 1. The fixing plate 3 limits the position of the solvent bottles 1, effectively preventing them from shaking or tipping over during the experiment, ensuring experimental safety and accuracy. A resistance layer 26 is provided at the contact point between the fixing plate 3 and the bottle box 2. The resistance layer 26 significantly increases the stability of the fixing plate 3 installed on the bottle box 2, preventing the fixing plate 3 from loosening due to external forces, thereby ensuring... The stable placement of solvent bottle 1 increases the stability of the fixing plate 3 installed on bottle box 2. The bottom of the fixing plate 3 is provided with a positioning hexagonal post 15, and the top of bottle box 2 is provided with a hexagonal groove 16 and a lower positioning groove 14 that are adapted to the positioning hexagonal post 15. The fixing plate 3 achieves a detachable connection with bottle box 2 through the cooperation of the positioning hexagonal post 15 with the hexagonal groove 16 and the lower positioning groove 14. The precise cooperation of the positioning hexagonal post 15 with the hexagonal groove 16 and the lower positioning groove 14 achieves a detachable connection between the fixing plate 3 and bottle box 2, which makes it convenient for experimental personnel to install or remove the fixing plate 3 according to their needs, improving the flexibility of equipment use. The side of bottle box 2 is provided with a side handle groove 4.

[0023] Please see Figure 1 Below the bottle box 2, a vacuum degasser 27, a quaternary pump 5, an autosampler 6, a column oven 7, and a detector 8 are connected in sequence. The vacuum degasser 27, the quaternary pump 5, the autosampler 6, the column oven 7, and the detector 8 are stacked vertically. This vertical stacking connection method effectively saves the equipment's floor space, makes the equipment structure more compact, and also facilitates the pipeline connection and signal transmission between the components, thereby improving the overall operating efficiency of the equipment.

[0024] Please see Figure 1 , Figure 3 and Figure 4Side cooling boxes 9 are connected to the right sides of the vacuum degasser 27, the quaternary pump 5, and the autosampler 6. Each side cooling box 9 contains a cooling housing 10, and an intelligent control screen 11 is mounted on its surface. The intelligent control screen 11 allows researchers to easily set and adjust the cooling parameters of the cooling housing 10, enabling intelligent control of the cooling temperature and meeting the cooling temperature requirements of the equipment under different experimental conditions. The cooling housing 10 is embedded within the side cooling box 9, and the intelligent control screen 11 is fitted into the outer surface of the cooling housing 10. Furthermore, the intelligent control screen 11 is connected to the internal control module of the equipment via wiring. This structural design tightly integrates the cooling box 10 with the side cooling box 9, ensuring cooling performance while guaranteeing stable communication between the intelligent control screen 11 and the internal control module for precise temperature control. A pull-out box 12 is connected to the side cooling box 9 via a pull-out slot 17. The cooperation between the pull-out slot 17 and the pull-out box 12 facilitates the removal and storage of items by experimental personnel, increasing the equipment's storage capacity and allowing for the categorized storage and management of experimental items. The pull-out box 12 contains a first clamping plate 18, a middle plate 21, a second clamping plate 23, and a compression spring 24. The first clamping plate 18 and the second clamping plate 23... The three components have identical structures and are symmetrically distributed. Clamping grooves 19 are provided on the first clamping plate 18 and the second clamping plate 23. All grooves 19 are arc-shaped and equidistant from each other along the length of the first clamping plate 18 and the second clamping plate 23. The arc-shaped grooves 19 better conform to the shape of the item, stably clamping items of different shapes and sizes, thus improving the applicability of the pull-out box 12. Rubber pads 20 are provided inside the grooves 19, increasing the friction between the grooves 19 and the item, further enhancing the fixing effect, and also acting as a buffer to prevent damage to the item. To prevent damage, a rubber pad 20 is provided inside the clamping groove 19, and a compression spring 24 is fitted with a limiting sleeve 25. One end of the compression spring 24 is connected to the second clamping plate 23, and the other end is connected to the left and right sides of the middle plate 21. The cooperation between the compression spring 24 and the limiting sleeve 25 can provide elastic clamping force for the first clamping plate 18 and the second clamping plate 23, and automatically adjust the clamping force according to the size of the item to ensure that the item is firmly fixed. The pull-out box 12 is provided with a handle 13, and the middle plate 21 is provided with a label 22. The label 22 makes it easy for the experimenter to identify the items in the pull-out box 12, facilitates quick retrieval and use of the required items, and improves experimental efficiency.

[0025] The implementation principle of the high-performance liquid chromatography-tandem mass spectrometry integrated instrument in this application embodiment is as follows: When the integrated instrument is working, the solvent bottle 1 is placed on top of the bottle box 2. The fixing plate 3 is detachably connected to the hexagonal groove 16 and the lower positioning groove 14 on the top of the bottle box 2 through the positioning hexagonal column 15. The resistance layer 26 at the contact part increases the installation stability. The fixing plate 3 limits the solvent bottle 1 to prevent shaking or tipping. The vacuum degasser 27, quaternary pump 5, autosampler 6, column oven 7, and detector 8 are stacked vertically below the bottle box 2 in sequence, which saves space and facilitates pipeline connection and signal transmission. Inside the side cooling box 9 connected to the right side of the injector 6, the experimenter sets the cooling parameters of the cooling box 10 through the intelligent control screen 11 to achieve intelligent temperature control. The side cooling box 9 is connected to the pull-out box 12 through the pull-out groove 17 to store test tubes. The rubber pads 20 in the arc-shaped grooves 19 on the first clamping plate 18 and the second clamping plate 23 inside the pull-out box 12 increase the friction with the test tubes. The compression spring 24 is fitted with the limiting cylinder 25 to provide elastic clamping force for the clamping plate. The force is automatically adjusted according to the size of the test tube to secure it firmly. The label 22 on the middle plate 21 makes it easy to identify the test tubes. The box handle 13 makes it easy to pull out. All the components work together to complete the high performance liquid chromatography tandem mass spectrometry detection experiment.

[0026] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A high-performance liquid chromatography-tandem mass spectrometry integrated system, comprising a vial box (2), characterized in that: The top of the bottle box (2) is used to place the solvent bottle (1). The bottle box (2) is provided with a fixing plate (3) for limiting the solvent bottle (1). The side of the bottle box (2) is provided with a side handle groove (4). The bottom of the bottle box (2) is connected in sequence with a vacuum degasser (27), a quaternary pump (5), an autosampler (6), a column oven (7), and a detector (8). The right side of the vacuum degasser (27), the right side of the quaternary pump (5), and the right side of the autosampler (6) are all connected with side cooling boxes (9). The side cooling box (9) is provided with a cooling box (10). The surface of the cooling box (10) is equipped with an intelligent control screen (11). The side cooling box (9) is connected to a pull box (12) through a pull groove (17). The pull box (12) is provided with a handle (13).

2. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 1, characterized in that: The bottom of the fixed plate (3) is provided with a positioning hexagonal post (15), and the top of the bottle box (2) is provided with a hexagonal groove (16) and a lower positioning groove (14) that are adapted to the positioning hexagonal post (15). The fixed plate (3) is connected to the bottle box (2) in a detachable manner by cooperating with the positioning hexagonal post (15), the hexagonal groove (16), and the lower positioning groove (14).

3. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 1, characterized in that: The pull-out box (12) is provided with a first clamping plate (18), a middle plate (21), a second clamping plate (23) and a compression spring (24). The first clamping plate (18) and the second clamping plate (23) are provided with clamping grooves (19). A rubber pad (20) is provided in the clamping grooves (19). The compression spring (24) is fitted with a limiting sleeve (25). One end of the compression spring (24) is connected to the second clamping plate (23) and the other end is connected to the left and right sides of the middle plate (21). A label (22) is provided on the middle plate (21).

4. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 1, characterized in that: The contact area between the fixing plate (3) and the bottle box (2) is provided with a resistance layer (26) to increase the stability of the fixing plate (3) installed on the bottle box (2).

5. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 1, characterized in that: The vacuum degasser (27), quaternary pump (5), autosampler (6), column oven (7), and detector (8) are stacked vertically in sequence.

6. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 1, characterized in that: The refrigeration box (10) is embedded in the internal space of the side cooling box (9), and the intelligent control screen (11) is inlaid and matched with the outer surface of the refrigeration box (10). The intelligent control screen (11) is connected to the internal control module of the equipment through a line.

7. The high-performance liquid chromatography-tandem mass spectrometry integrated system according to claim 3, characterized in that: The No. 1 clamping plate (18) and the No. 2 clamping plate (23) have the same structure and are symmetrically distributed. The multiple clamping grooves (19) are all arc-shaped and are arranged at equal intervals along the length of the No. 1 clamping plate (18) and the length of the No. 2 clamping plate (23).

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