A miniature solution content detection device
By designing a miniature solution content detection device, the problems of reliance on large equipment and complex operation in existing technologies have been solved, realizing miniaturized real-time online detection and improving detection efficiency and applicability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHENZHEN HUIXIN LIFE TECH CO LTD
- Filing Date
- 2025-09-11
- Publication Date
- 2026-08-04
AI Technical Summary
Existing methods for detecting contents in solutions rely on large-scale equipment, cannot perform real-time online detection, are complex to operate, consume chemical reagents, have long detection times, and require large sample volumes, which cannot meet the needs of micro-samples.
A miniature solution content detection device is designed, including a base, a photosensitive component, and a sample storage device. It adopts a specific wavelength absorption method and is integrated into the sample solution delivery pipeline. An anti-glare coating reduces stray light interference. The detection area is designed as an expansion zone, a detection zone, and a contraction zone to achieve miniaturization and real-time online detection.
It achieves miniaturized real-time online detection with a single-point detection time of less than 1 second, requiring very few samples, thus reducing production costs and improving detection efficiency and applicability.
Smart Images
Figure CN224594474U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to a micro solution content detection device, belonging to the field of content detection technology. Background Technology
[0002] Currently, in the field of solution content detection, especially in the concentration analysis of biomedical samples (such as proteins and nucleic acids), traditional detection methods mainly rely on techniques such as the Qubit method and the BCA method. These methods generally suffer from the following drawbacks: First, current detection methods are highly dependent on equipment and require large, specialized equipment, resulting in bulky detection systems that cannot be integrated into continuous flow paths. Second, manual sampling must be performed before detection, making the process complex and unable to meet the needs of real-time online detection. Furthermore, existing detection methods consume chemical reagents and undergo an incubation phase, leading to high detection costs and excessively long single-point detection times, resulting in low detection efficiency. Finally, current detection methods require excessively large sample volumes per detection, necessitating pre-dilution of trace samples, leading to concentration distortion and sample waste. Therefore, researching a novel micro-sized solution content detection device is of great practical significance. Utility Model Content
[0003] This invention addresses the shortcomings of existing technologies by providing a micro-sized solution contents detection device.
[0004] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: A micro solution contents detection device includes: a base, the base being a hollow structure with a through-hole for mounting, the two side walls of the mounting cavity being respectively provided with a first mounting hole and a second mounting hole communicating with the mounting cavity, the first mounting hole and the second mounting hole being coaxially opposite to each other;
[0005] A light-sensing component includes a light source and a sensor for receiving light beam signals, wherein the light source is disposed in a first mounting hole and the sensor is disposed in a second mounting hole;
[0006] The sample storage device includes a detection chamber and an inlet pipe and an outlet pipe communicating with the detection chamber. The detection chamber is disposed within the mounting cavity, and the side wall of the detection chamber is made of a light-transmitting material. The light beam emitted by the light source passes through the detection chamber.
[0007] Furthermore, the light-sensing component also includes a first shield and a second shield. The first shield is fixed to the side wall of the base, and its surface is in contact with the surface of the side wall of the base. The light source is disposed on the first shield.
[0008] The second shield is fixed to the side wall of the base, and its surface is in contact with the surface of the side wall of the base. The sensor is disposed on the second shield.
[0009] Furthermore, the sample storage device is confined within the mounting cavity by an end plate, which is sleeved outside the inlet pipe and / or the outlet pipe.
[0010] Furthermore, the sidewall surfaces of the detection chamber, except for the beam penetration area, are coated with a matte coating.
[0011] Furthermore, the thickness of the matte coating is 5-50 micrometers.
[0012] Furthermore, the detection chamber is provided with a detection cavity, which is composed of an expansion zone located at the outlet of the liquid inlet pipe, a detection zone, and a contraction zone located at the inlet of the liquid outlet pipe, which are connected in sequence. The cross-sectional area of the expansion zone gradually increases along the liquid inlet direction, and the cross-sectional area of the contraction zone gradually decreases along the liquid inlet direction.
[0013] Furthermore, the expansion zone, detection zone, and contraction zone all have rectangular cross-sectional shapes in the direction perpendicular to the inlet pipe axis; the outer contour of the detection chamber and the cross-sectional shape of the mounting cavity are both square. By changing the installation position of the sample storage device, the light beam emitted by the light source can pass through the short side of the rectangular cross-section or through the long side of the rectangular cross-section.
[0014] Furthermore, the width L1 of the short side of the detection area is 0.2-2 times the length L2 of the long side.
[0015] Furthermore, the expansion zone and the contraction zone have the same inclination angle relative to the axis of the inlet pipe, both ranging from 15° to 80°.
[0016] The beneficial effects of this utility model are as follows: First, it solves the problem that previous detection methods required large equipment and process interruptions before detection, which prevented real-time online detection. Through the above-mentioned design, the detection device described in this application achieves miniaturization while ensuring detection accuracy, allowing it to be integrated into the sample solution delivery pipeline and meeting the requirements for real-time online detection. Second, it uses a specific wavelength absorption method to detect the concentration of contents. The device is compact and highly reliable, requiring no reaction reagents or incubation time during detection. The single-point detection time can be controlled to <1 second, effectively improving detection efficiency and reducing production and operating costs. Finally, the detection device described in this application requires a very small sample volume for a single detection, and the sample does not need to be diluted beforehand. There is no sample loss after detection, making it suitable for detecting contents in trace samples, effectively expanding its application range and further improving detection efficiency. Attached Figure Description
[0017] Figure 1 This is a three-dimensional structural diagram of the detection device provided in Embodiment 1 of this utility model;
[0018] Figure 2 This is an exploded view of the detection device provided in Embodiment 1 of this utility model;
[0019] Figure 3 This is a schematic diagram of the three-dimensional structure of the base provided in Embodiment 1 of this utility model;
[0020] Figure 4 This is a three-dimensional structural diagram of the sample storage device provided in Embodiment 1 of this utility model;
[0021] Figure 5 This is a front view of the sample storage device provided in Embodiment 1 of this utility model;
[0022] Figure 6 for Figure 5 Sectional view along the DD direction;
[0023] Figure 7 for Figure 5 Cross-sectional view along the BB direction.
[0024] Reference numerals: 1. Base; 11. Mounting cavity; 12. First mounting hole; 13. Second mounting hole; 2. Light source; 3. Sensor; 4. Sample storage device; 41. Detection chamber; 411. Expansion zone; 412. Detection zone; 413. Contraction zone; 42. Inlet pipe; 43. Outlet pipe; 5. First baffle; 6. Second baffle; 7. End plate. Detailed Implementation
[0025] The specific embodiments of this utility model are described in detail below. This utility model can be implemented in many ways different from those described herein, and those skilled in the art can make similar improvements without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed herein.
[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used is for describing particular embodiments only and is not intended to limit the scope of this invention.
[0027] In the description of this utility model, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.
[0028] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0029] Example 1
[0030] like Figure 1-4 As shown, this utility model provides a miniature solution content detection device, comprising: a base 1, the base 1 being a hollow structure with a through mounting cavity 11, the mounting cavity 11 having a first mounting hole 12 and a second mounting hole 13 respectively on its two side walls, the first mounting hole 12 and the second mounting hole 13 being coaxially opposite to each other; a photosensitive component, comprising a light source 2 and a sensor 3 for receiving light beam signals, the light source 2 being disposed in the first mounting hole 12 and the sensor 3 being disposed in the second mounting hole 13; and a sample storage component 4, comprising a detection chamber 41 and an inlet pipe 42 and an outlet pipe 43 communicating with the detection chamber 41, the detection chamber 41 being disposed within the mounting cavity 11, and the side wall of the detection chamber 41 being made of a light-transmitting material, the light beam emitted by the light source 2 passing through the detection chamber 41. It should be noted that the inlet pipe 42, the outlet pipe 43, and the detection chamber 41 are integral structures manufactured together. It can be understood that the light source 2, the sensor 3, and the detection chamber 41 are in a straight line. The light beam emitted by the light source 2 passes through the detection chamber 41 and is received by the sensor 3. When detecting the contents of the solution, the sample solution enters the detection chamber 41 through the inlet pipe 42 under the drive of external power. The external power is a pump known to those skilled in the art, which will not be described in detail here. At this time, the light beam emitted by the light source 2 irradiates and passes through the sample solution. When the light beam passes through the sample solution, the components to be detected in the solution will selectively absorb light of a specific wavelength. Then the sensor 3 receives the light signal and shows an absorption peak in the spectrum. The staff can then determine the concentration of the contents to be detected by comparing the absorption peak height with the standard value.
[0031] The above settings firstly solve the problem of relying on large equipment and interrupting the process before detection, which prevents real-time online detection. These settings enable the detection device described in this application to be miniaturized while ensuring detection accuracy, allowing it to be integrated into the sample solution delivery pipeline and meeting the requirements for real-time online detection. Secondly, by using a specific wavelength absorption method to detect the concentration of contents, the device is compact and highly reliable. No reaction reagents or incubation time are required during the detection process, and the single-point detection time can be controlled to <1 second, effectively improving detection efficiency and reducing production and operating costs. Finally, the detection device described in this application requires a very small sample volume for a single detection, and the sample does not need to be diluted beforehand. There is no sample loss after detection, making it suitable for detecting contents in trace samples, effectively expanding its application range and further improving detection efficiency.
[0032] Specifically, such as Figure 1-2 As shown, the photosensitive component also includes a first shield 5 and a second shield 6. The first shield 5 is fixed to the side wall of the base 1 by screws, bolts, welding, etc., and its surface is in contact with the side wall surface of the base 1. The light source 2 is disposed on the first shield 5, and the first shield 5 is also provided with a signal line slot. The second shield 6 is fixed to the side wall of the base 1 by screws, bolts, welding, etc., and its surface is in contact with the side wall surface of the base 1. The sensor 3 is disposed on the second shield 6, and the second shield 6 is also provided with a signal line slot. This arrangement can effectively block ambient light, preventing ambient light from seeping into the mounting cavity 11 through the mounting gap between the light source 2 and the first mounting hole 12 and the mounting gap between the sensor 3 and the second mounting hole 13, reducing the influence of ambient light on the detection results, effectively improving the signal-to-noise ratio, and ensuring the accuracy of the detection results.
[0033] Specifically, such as Figure 1-2 As shown, the sample storage device 4 is confined within the mounting cavity 11 by an end plate 7, which is sleeved around the inlet pipe 42 and / or the outlet pipe 43. The end plate 7 is fixed to the opening of the mounting cavity 11 by bolts or screws, which prevents the sample storage device 4 from shaking during the testing process and affecting the test results, and also facilitates the subsequent disassembly of the sample storage device 4 for maintenance.
[0034] Specifically, to further reduce the interference of non-signal light such as scattered light from the sidewalls of the flow chamber and ambient light on the detection results, the sidewall surfaces of the detection chamber 41, except for the beam penetration area, are coated with a matting coating. It should be noted that the matting coating is made of ultrafine silica or stearate. This effectively improves the absorption rate of stray light, further ensuring detection accuracy. Preferably, the thickness of the matting coating is 5-50 micrometers. This parameter is crucial. If the thickness is less than 5 micrometers, the surface roughness of the sample storage device 4 will lead to uneven coating coverage, forming micropores. Stray light passing through these micropores will reduce the absorption rate of stray light. If the thickness is greater than 50 micrometers, the coating and the outer surface of the sample storage device 4 will experience interfacial peeling under temperature fluctuations, causing the coating to peel off during cleaning and resulting in structural failure. Only when the thickness of the matting coating is 5-50 micrometers can both a stray light absorption rate >97% and a coating cleaning life >3000 cycles be ensured.
[0035] Specifically, such as Figure 7 As shown, the detection chamber 41 has a detection cavity inside. The detection cavity is composed of an expansion area 411 located at the outlet of the liquid inlet pipe 42, a detection area 412, and a contraction area 413 located at the inlet of the liquid outlet pipe 43 connected in sequence. The cross-sectional area of the expansion area 411 gradually increases along the liquid inlet direction, and the cross-sectional area of the contraction area 413 gradually decreases along the liquid inlet direction. It should be noted that the expansion zone 411, detection zone 412, and contraction zone 413 are integral structures formed by processing. In the attached figure, the dashed line X1 is the central axis of the sample storage device 4, and the other vertical dashed lines are only used as indicator lines to clearly show the positional distribution of the expansion zone 411, detection zone 412, and contraction zone 413. Through the above settings, this application can first ensure that the flow of the solution to be tested smoothly transitions from a high-kinetic-energy flow state to a laminar flow state, avoiding the problem of excessive bubbles being generated at the connection transition position due to structural abrupt changes, and solving the problem of reduced detection accuracy due to the influence of excessive bubbles on the optical path. Secondly, when the solution to be tested enters the detection zone 412 after deceleration, the proteins contained therein are easily adsorbed and deposited on the wall surface. By setting the gradually narrowing contraction zone 413, when the solution enters the contraction zone 413 from the detection zone 412, the flow rate will gradually increase, generating a certain shear force, thereby washing away the adsorbed and deposited proteins, effectively reducing the amount of sample residue and reducing the probability of cross-contamination of the sample solution.
[0036] Specifically, such as Figure 6As shown, the expansion area 411, detection area 412 and contraction area 413 are all rectangular in cross-sectional shape in the direction perpendicular to the axis of the inlet pipe 42; the outer contour of the detection chamber 41 and the cross-sectional shape of the mounting cavity 11 are both square. By changing the installation position of the sample storage device 4, the light beam emitted by the light source 2 can pass through the short side of the rectangular cross-section or through the long side of the rectangular cross-section. It is understood that by changing the installation position of the sample storage device 4, i.e., rotating the sample storage device 4 90 degrees around its axis, the contents detection device of this application achieves a dual optical path structure design, ensuring high detection accuracy when applicable to sample solutions of different concentrations. While ensuring detection accuracy, the applicability is further improved. When detecting sample solutions with high concentrations, the installation position of the sample storage device 4 is adjusted so that the detection device of this application is in a short optical path detection state. At this time, the light beam emitted by the light source 2 penetrates the long side of the rectangular cross-section. When detecting sample solutions with low concentrations, the installation position of the sample storage device 4 is adjusted so that the detection device of this application is in a long optical path detection state. At this time, the light beam emitted by the light source 2 penetrates the short side of the rectangular cross-section.
[0037] Specifically, such as Figure 6 As shown, the width L1 of the short side of the detection area 412 is 0.2-2 times, preferably 0.4-0.6 times, the length L2 of the long side. When detecting trace amounts of solution with a sample volume <100 μL, the surface tension of the sample solution can cause uneven flow. Furthermore, when the sample solution enters the detection area 412, it forms a preferential flow channel in the narrower width direction, resulting in excessively high flow velocity at the center of the detection area 412, while stagnant areas form on the sides. This uneven flow velocity causes sample contents to accumulate on the sides, leading to larger local concentration deviations and reduced detection accuracy. If the detection area 412 has a rectangular cross-section... When the width L1 of the short side of the surface is less than 0.2 times the length L2 of the long side, the flow channel will be too narrow, which will increase the wall friction and prolong the retention time of the sample at the edge. Only when the width L1 of the short side of the detection area 412 is 0.2-2 times the length L2 of the long side can the relationship between the flow velocity and the flow state be balanced while ensuring the accuracy of the detection when detecting trace solution contents with a sample volume of <100μL. This will improve the uniformity of the sample solution flow velocity distribution to ≥90%, and further improve the adaptability of the device.
[0038] Specifically, such as Figure 7As shown, the expansion zone 411 and the contraction zone 413 have the same inclination angle α relative to the axis of the inlet pipe 42, which is 15°-80°, preferably 35°-45°. By limiting the inclination angle, this application can first suppress the generation of sample solution eddies, allowing the sample solution to be tested to fill the detection zone 412 more smoothly, effectively avoiding the problem of bubble generation. Secondly, during cleaning, the cleaning solution can completely cover the entire detection chamber, effectively reducing the amount of sample solution residue and further avoiding the problem of cross-contamination. If the inclination angle α is less than... At 15°, the liquid flow transition is too gentle. After entering the detection zone 412, the liquid flow of the sample to be tested still maintains a high inertial force, causing the liquid flow to directly impact the wall of the detection zone 412 and form a splash vortex. This results in too many bubbles being generated in the sample solution, affecting the detection results. If the tilt angle α is greater than 80°, the connection position between the expansion zone 411 and the contraction zone 413 and the detection zone 412 forms an approximately right-angle transition zone. When the liquid flow passes through, it is very easy to cause turbulence due to the sudden change in cross-section, and it will also increase the residual amount of sample solution.
[0039] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are exhaustively listed. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0040] For those skilled in the art, various modifications and improvements can be made without departing from the concept of this utility model, and these modifications and improvements are all within the protection scope of this utility model. The protection scope of this utility model is defined by the appended claims.
Claims
1. A miniature solution contents detection device, characterized in that, include: The base is a hollow structure with a through-hole for mounting. The two side walls of the mounting cavity are respectively provided with a first mounting hole and a second mounting hole that communicate with the mounting cavity. The first mounting hole and the second mounting hole are coaxially opposite to each other. A light-sensing component includes a light source and a sensor for receiving light beam signals, wherein the light source is disposed in a first mounting hole and the sensor is disposed in a second mounting hole; The sample storage device includes a detection chamber and an inlet pipe and an outlet pipe communicating with the detection chamber. The detection chamber is disposed within the mounting cavity, and the side wall of the detection chamber is made of a light-transmitting material. The light beam emitted by the light source passes through the detection chamber.
2. The micro solution contents detection device according to claim 1, characterized in that, The light-sensing component also includes a first shield and a second shield. The first shield is fixed to the side wall of the base, and its surface is in contact with the surface of the side wall of the base. The light source is disposed on the first shield. The second shield is fixed to the side wall of the base, and its surface is in contact with the surface of the side wall of the base. The sensor is disposed on the second shield.
3. The micro solution contents detection device according to claim 2, characterized in that, The sample storage device is confined within the mounting cavity by an end plate, which is sleeved outside the inlet pipe and / or the outlet pipe.
4. The micro solution contents detection device according to claim 3, characterized in that, The sidewalls of the testing chamber, except for the area through which the light beam passes, are coated with a matte coating.
5. A micro solution contents detection device according to claim 4, characterized in that, The thickness of the matte coating is 5-50 micrometers.
6. A micro solution contents detection device according to any one of claims 1-4, characterized in that, The detection chamber is provided with a detection cavity, which is composed of an expansion zone and a detection zone located at the outlet of the liquid inlet pipe and a contraction zone located at the inlet of the liquid outlet pipe connected in sequence. The cross-sectional area of the expansion zone gradually increases along the liquid inlet direction, and the cross-sectional area of the contraction zone gradually decreases along the liquid inlet direction.
7. A micro solution contents detection device according to claim 6, characterized in that, The expansion zone, detection zone, and contraction zone all have rectangular cross-sectional shapes in the direction perpendicular to the axis of the inlet pipe; The outer contour of the detection chamber and the cross-sectional shape of the mounting cavity are both square. By changing the installation position of the sample storage device, the light beam emitted by the light source can pass through the short side of the rectangular cross-section or through the long side of the rectangular cross-section.
8. A micro solution contents detection device according to claim 7, characterized in that, The width L1 of the short side of the detection area is 0.2-2 times the length L2 of the long side.
9. A micro solution contents detection device according to claim 8, characterized in that, The expansion zone and the contraction zone have the same inclination angle relative to the inlet pipe axis, which is 15°-80°.