A combined cell freezing container

Abstract

The utility model discloses a combined cell cryopreservation container belongs to biomedical experiment cell cryopreservation technical field. Mainly include two infusion pipeline and a plurality of cryopreservation pipeline, the infusion pipeline includes annular pipeline, one side of annular pipeline is connected with the concentrated pipeline, the other side of annular pipeline is connected with a plurality of branch liquid line, the cryopreservation pipeline includes the cryopreservation tube, the lower end of cryopreservation tube is connected with the liquid supply pipeline, the upper end of cryopreservation tube is connected with the liquid outlet pipeline, the liquid supply pipeline and liquid outlet pipeline are connected respectively with the branch liquid line on one infusion pipeline through the luer joint. A combined cell cryopreservation container of the present application solves the existing cell cryopreservation specification fixed, the partial cell remains waste, the flexibility is poor, and the adaptability is insufficient etc.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental cell cryopreservation technology, and more specifically, it relates to a combined cell cryopreservation container. Background Technology

[0002] In cell biology research, regenerative medicine, and biopharmaceuticals, cell cryopreservation is a key technology for long-term preservation of cell viability, and the performance of cryopreservation containers directly affects the cryopreservation effect and operational safety. Currently, the mainstream cell cryopreservation containers on the market are mainly fixed-size cell cryopreservation tubes (such as 2mL, 5mL) and cell cryopreservation bags (such as CS50, CS250), but they have the following significant defects and shortcomings: 1. Fixed specifications and low dispensing efficiency: Existing containers have a fixed capacity, making it impossible to uniformly dispense large quantities of cells in a single operation. Each container / bag needs to be dispensed individually, which is not only time-consuming but also prone to uneven dispensing due to differences in operation.

[0003] 2. The recovery process is inconvenient and carries risks: The 5mL cryopreservation tube is relatively long, making it difficult for the syringe needle to reach the bottom of the tube after thawing, resulting in some cells remaining and being wasted.

[0004] Cell cryopreservation bags are made of a soft material, which can easily puncture the bag when drawn directly with a syringe, causing cell leakage, environmental contamination, and sample loss. 3. Technical challenges exist in the cryopreservation process: Cryopreservation bags require manual removal of air bubbles, which is cumbersome and can easily affect the freezing effect due to residual air bubbles. When the volume is less than the cryopreservation bag specification range (e.g., 15mL is only filled in a 10-30mL specification), the cells cannot be evenly spread out, resulting in uneven temperature distribution during programmed cooling, which affects the cryopreservation effect and cell viability. 4. Poor flexibility and insufficient adaptability: The cell volume varies greatly under different experimental needs. The existing fixed-size containers cannot flexibly adjust the number of cryopreservation units according to the cell volume. Excessive or insufficient filling will affect the cryopreservation quality. Moreover, if a single unit / bag is damaged, the whole unit must be discarded, which is costly and easily leads to the problem of "using too much material for too little" or "insufficient filling", increasing the experimental cost.

[0005] 5. Difficulty in batch management: During large-scale cryopreservation, multiple independent cryopreservation tubes or bags need to be individually labeled and stored, which can easily lead to confusion, occupy liquid nitrogen tank storage space, and result in low efficiency when retrieving them. Therefore, it is necessary to provide a modular cell cryopreservation container to solve the above problems. Utility Model Content

[0006] Based on the above-mentioned problems in the existing technology, the purpose of this application is to provide a combined cell cryopreservation container, which solves the problems of fixed cell cryopreservation specifications, low dispensing efficiency, risks of direct aspiration of cryopreservation bags with syringes, waste of some residual cells, poor flexibility, and insufficient adaptability.

[0007] The technical solution adopted by this application to solve its technical problem is: a combined cell cryopreservation container, including two infusion lines and several cryopreservation lines; The infusion pipeline includes a ring pipeline, one side of which is connected to a central pipeline, and the other side of which is connected to several distribution pipelines. The cryopreservation tubing includes a cryopreservation tube, the lower end of which is connected to a liquid supply line, and the upper end of which is connected to a liquid outlet line. The liquid supply line and the liquid outlet line are respectively connected to a dispensing line on an infusion line via Luer connectors.

[0008] Furthermore, one of the infusion lines is equipped with female Luer connectors, the other infusion line is equipped with male Luer connectors, the supply line is equipped with a female Luer connector, and the outlet line is equipped with a male Luer connector.

[0009] Furthermore, each of the liquid distribution lines is equipped with an infusion hose clamp.

[0010] Furthermore, the upper end of the cryopreservation tube is connected to a breathing tube, and the inside of the breathing tube is lined with breathing cotton.

[0011] Furthermore, a breathing hose clamp is also provided on the breathing tubing.

[0012] The beneficial effects of this utility model are: 1. Flexible adaptation and batch operation: The number of cryopreservation units can be freely combined in series or parallel (5 segments, 10 segments, 20 segments, etc.) to meet different cell volume requirements. The multi-unit parallel design enables one-time large-batch liquid addition and uniform dispensing, reducing repeated operations, lowering the risk of contamination, and improving experimental efficiency. 2. Improve cryopreservation and thawing effects: Each cryopreservation tube contains a fixed volume of 5mL to ensure uniform cell distribution, consistent temperature during programmed cooling, and preservation of cryopreservation viability. The inlet and outlet are designed to be safe and complete, allowing for safe aspiration of contents during resuscitation through the top or bottom interface, avoiding residue and waste. The rigid tube replaces the soft bag, eliminating the risk of puncture leakage. Standardized loading ensures uniform cell distribution and consistent temperature distribution during programmed cooling. 3. Operational safety and convenience: EVA piping supports heat sealing, and the interfaces are equipped with hose clamps to ensure sterility and tightness; The breathable cotton filter port venting design reduces manual defoaming steps, lowers operational errors, balances pressure, and prevents contamination during liquid addition; The modular design allows for flexible adjustment of the number of units based on cell quantity, and individual units can be replaced when a single segment is damaged, reducing usage costs and the risk of sample loss. 4. Compatibility and economy: It uses commercially available cryopreservation materials (resistant to low temperatures and DMSO), Luer connectors and EVA tubing, and is compatible with existing handheld heat sealers and mainstream cell cryopreservation procedures. The modular design reduces material waste and lowers long-term experimental costs. 5. Optimized packaging process: The matching liquid adding rack is suitable for liquid adding and freezing operations, facilitating batch dispensing operations. Attached Figure Description

[0013] The accompanying drawings, which form part of this specification, are used to provide a further understanding of this utility model. The illustrative embodiments and descriptions of this utility model are used to explain this utility model and do not constitute an undue limitation thereof. In the drawings: Figure 1 This is an overall schematic diagram of a modular cell cryopreservation container according to this application; Figure 2 for Figure 1 A schematic diagram of the series connection of the cryopreservation pipeline; In the picture: 1. Infusion tubing; 11. Loop tubing; 12. Centralized tubing; 13. Distributed tubing; 14. Infusion hose clamps; 2. Cryopreservation tubing; 21. Cryopreservation tubing; 22. Liquid supply tubing; 23. Liquid outlet tubing; 24. Breathing tubing; 25. Breathing cotton; 26. Breathing tubing clamp. Detailed Implementation

[0014] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0015] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0016] In this utility model, unless otherwise stated, the orientations used, such as "up" and "down", usually refer to the direction shown in the accompanying drawings, or to the vertical, perpendicular, or gravitational direction; similarly, for ease of understanding and description, "left" and "right" usually refer to the left and right shown in the accompanying drawings; "inner" and "outer" refer to the inner and outer contours of each component itself, but the above directional terms are not used to limit this utility model.

[0017] like Figure 1-2 As shown, this application provides a combined cell cryopreservation container, including two infusion lines 1 and several cryopreservation lines 2.

[0018] The infusion line 1 includes a ring line 11, one side of which is connected to a central line 12, and the other side of which is connected to several distribution lines 13. Each distribution line 13 is equipped with an infusion hose clamp 14. Each distribution line 13 is equipped with a Luer connector at its end. One infusion line 1 is equipped with female Luer connectors, and the other infusion line 1 is equipped with male Luer connectors.

[0019] The cryopreservation tubing 2 includes a cryopreservation tube 21. The lower end of the cryopreservation tube 21 is connected to a supply tubing 22, and the upper end of the cryopreservation tube 21 is connected to an outlet tubing 23 and a breathing tubing 24. The breathing tubing 24 is internally fitted with a breathing cotton 25 and also has a breathing hose clamp 26. Both the supply tubing 22 and the outlet tubing 23 are equipped with Luer connectors at their ends. In this embodiment, the supply tubing 22 has a female Luer connector, and the outlet tubing 23 has a male Luer connector.

[0020] The Luer connector is a standardized micro-leakage-free connector in the prior art, which is connected by a male Luer connector and a matching female Luer connector. Therefore, the supply line 22 and the outlet line 23 can be connected to the distribution line 13 on a delivery line 1, respectively.

[0021] Cryopreservation tube 21 is an independent 5mL cryopreservation tube with a circular shape and graduations, and its diameter is consistent with the existing best cryopreservation tubes. The entire cryopreservation tubing 2 is made of a high-performance material that can withstand the low temperature of liquid nitrogen down to -196℃ (same as the materials of existing high-quality cryopreservation tubes, such as cryopreservation tube 21 being made of polycarbonate PC, and supply tubing 22, outlet tubing 23 and breathing tubing 24 being made of EVA material, compatible with commonly used cell cryopreservation cryoprotectants such as DMSO).

[0022] Connect cryopreservation tubing 2 and infusion tubing 1 as follows: Figure 1As shown, when in use, open the infusion tubing clamp 14 and the breathing tubing clamp 26. At this time, the cryopreservation solution is introduced through the central tubing 12 of the lower infusion tubing 1. It is then diverted into each cryopreservation tubing 2 through the annular tubing 11 and the dispensing tubing 13 of the lower infusion tubing 1. After that, close all tubing clamps and seal each joint with a heat sealer to complete the dispensing.

[0023] Because the supply line 22 is equipped with a female Luer connector and the outlet line 23 is equipped with a male Luer connector, the cryopreservation lines 2 can be connected in series. When there are many cryopreservation lines 2, it is possible to... Figure 2 As shown in the figure, multiple cryopreservation tubing 2 are connected in series and then connected to infusion tubing 1.

[0024] In summary, by freely combining the number of cryopreservation units in series and parallel, different cell volume requirements can be met. The multi-unit parallel design enables one-time large-batch liquid addition and uniform dispensing, reducing repetitive operations, lowering the risk of contamination, and improving experimental efficiency.

[0025] Each cryopreservation tube contains a fixed volume of 5 mL to ensure uniform cell distribution and consistent temperature during programmed cooling, thus guaranteeing cell viability. During thawing, the contents can be safely and completely aspirated through the top or bottom connector, avoiding residue and waste. The rigid tube body replaces the soft bag body, eliminating the risk of leakage during puncture. Standardized volume ensures even cell distribution and consistent temperature distribution during programmed cooling.

[0026] When there is gas in the cryopreservation tubing 2, the breathing tubing clamp 26 can be removed and the air can be vented through the filter interface of the breathing cotton 25. This design reduces the manual degassing steps, reduces operational errors, balances pressure, and avoids contamination during liquid addition.

[0027] EVA piping supports heat sealing, and the interfaces are equipped with hose clamps to ensure sterility and tightness.

[0028] The modular design allows for flexible adjustment of the number of units based on cell quantity, and individual units can be replaced if a single segment is damaged, reducing usage costs and the risk of sample loss.

[0029] It uses commercially available cryopreservation materials (resistant to low temperatures and DMSO), Luer interfaces and EVA tubing, and is compatible with existing handheld heat sealers and mainstream cell cryopreservation procedures. The modular design reduces material waste and lowers long-term experimental costs.

[0030] The matching liquid adding rack is suitable for liquid adding and freezing operations, facilitating batch dispensing operations.

[0031] Obviously, the embodiments described above are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of this utility model.

[0032] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0033] It should be noted that the terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of this application described herein can be implemented in sequences other than those illustrated or described herein.

[0034] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

[0035] The above description is merely a preferred embodiment of this utility model. The protection scope of this utility model is not limited to the above embodiments. All technical solutions falling within the scope of this utility model's concept are protected. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of this utility model should also be considered within the protection scope of this utility model.

Claims

1. A modular cell cryopreservation container, characterized in that: It includes two infusion lines (1) and several cryopreservation lines (2); The infusion line (1) includes a ring line (11), one side of which is connected to a central line (12), and the other side of which is connected to several distribution lines (13). The cryopreservation pipeline (2) includes a cryopreservation tube (21), the lower end of which is connected to a liquid supply pipeline (22), and the upper end of which is connected to a liquid outlet pipeline (23). The liquid supply pipeline (22) and the liquid outlet pipeline (23) are respectively connected to a liquid distribution pipeline (13) on an infusion pipeline (1) via Luer connectors.

2. The combined cell cryopreservation container according to claim 1, characterized in that: One of the infusion lines (1) is equipped with a female Luer connector, and the other infusion line (1) is equipped with a male Luer connector. The supply line (22) is equipped with a female Luer connector, and the outlet line (23) is equipped with a male Luer connector.

3. The combined cell cryopreservation container according to claim 1, characterized in that: Each of the liquid distribution lines (13) is equipped with a liquid infusion hose clamp (14).

4. A combined cell cryopreservation container according to claim 1, characterized in that: The upper end of the cryopreservation tube (21) is also connected to a breathing tube (24), and the inside of the breathing tube (24) is provided with breathing cotton (25).

5. A combined cell cryopreservation container according to claim 4, characterized in that: The breathing tubing (24) is also equipped with a breathing hose clamp (26).

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