A filter tip for immune repertoire assay

Abstract

This utility model relates to the field of filter cartridge tips, and discloses a filter cartridge tip for immune repertoire testing, including a conical part. A replacement mechanism is provided on the outer wall of the conical part. A body is fixedly connected to the bottom of the outer wall of the conical part, and an anti-snagging mechanism is provided on the inner wall of the body. The replacement mechanism includes a first fixing ring, the inner wall of which is fixedly connected to the outer wall of the conical part. A second fixing ring is fixedly connected to the outer wall of the conical part. A first leaf is fixedly connected to the bottom of the outer wall of the conical part, and a fixing groove is formed on the outer wall of the first leaf. In this utility model, the fixing ring and the fixing groove of the leaf cooperate, the sealing groove and sealing strip of the leaf enhance the sealing effect, the rotating component assists the movement of the leaf, and the reinforcing ring and reinforcing groove of the fixing component fix the leaf to prevent loosening and leakage of test liquid. The leaf design facilitates adaptation to different conical parts, and the entire mechanism allows for the replacement of the conical part according to the concentration and type of test liquid, improving the flexibility of use.

Description

Technical Field

[0001] This utility model relates to the field of filter cartridge tip technology, and in particular to a filter cartridge tip for immune repertoire testing. Background Technology

[0002] Immunome repertoire assays are experimental techniques used to study the gene sequences of immune cell receptors in immune structures. They can analyze the diversity and dynamic changes of immune cells, providing crucial data for disease diagnosis, treatment, and research on immune mechanisms. Precise handling of trace biological samples is required to ensure reliable results. These assays have extremely high requirements for sample contamination prevention. Conventional pipette tips can cause cross-contamination, affecting data accuracy. Therefore, specialized filter tips for immunoome repertoire assays are required. Their built-in filters can prevent sample backflow, effectively avoiding contamination and ensuring the smooth progress of the assay.

[0003] Traditional filter-cartridge pipette tips for immunoreproducible repertoire assays work by filtering liquids using a built-in filter cartridge. The negative pressure generated by the pipette tip draws the test liquid in, and the filter cartridge captures specific components. This traditional device suffers from low filtration efficiency, clogging, difficult cleaning, and results bias, among other drawbacks. Existing filter-cartridge pipette tips for immunoreproducible repertoire assays have optimized their operating principle, for example, by using high-efficiency filter media and improved fluid design to enhance filtration performance and user experience. However, in practical use, these devices lack a replacement mechanism. This structural deficiency prevents the device from adapting to different concentrations and types of test liquids, thus limiting its application flexibility and experimental accuracy. Therefore, a new filter-cartridge pipette tip for immunoreproducible repertoire assays is proposed to address these issues. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a filter cartridge tip for immune repertoire testing, aiming to improve the problem that the existing technology cannot adapt to different concentrations and types of test liquids, thus limiting its application flexibility and experimental accuracy.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a filter cartridge tip for an immune repertoire test, comprising a cone, a replacement mechanism provided on the outer wall of the cone, a body fixedly connected to the bottom of the outer wall of the cone, and an anti-snagging mechanism provided on the inner wall of the body; The replacement mechanism includes a first fixing ring, the inner wall of which is fixedly connected to the outer wall of the cone portion; a second fixing ring is fixedly connected to the outer wall of the cone portion; a first leaf is fixedly connected to the bottom of the outer wall of the cone portion; a fixing groove and a sealing groove are provided on the outer wall of the first leaf; a sealing strip is fixedly connected to the outer wall of the first leaf; a second leaf is fixedly connected to the outer wall of the first leaf; a rotating component and a fixing component are provided on the outer wall of the first leaf.

[0006] As a further description of the above technical solution: The anti-snagging mechanism includes a first filter screen, the outer wall of which is fixedly connected to the inner wall of the body. The inner wall of the body has a first engaging groove and a second engaging groove. The inner wall of the body is fixedly connected to a second filter screen. The inner wall of the body has gradient patterns and spiral channels. The inner wall of the body is provided with a flow-facilitating component.

[0007] As a further description of the above technical solution: The anti-wall-hanging mechanism also includes filter cotton, the outer wall of which is fixedly connected to the inner wall of the body.

[0008] As a further description of the above technical solution: The rotating assembly includes a first rotating shaft, the outer wall of which is fixedly connected to the outer wall of the first leaf, and a second rotating shaft is fixedly connected to the outer wall of the second leaf.

[0009] As a further description of the above technical solution: The fixing component includes a reinforcing ring, the outer wall of which is fixedly connected to the outer wall of the first leaf lobe, and the outer wall of the first leaf lobe is provided with a reinforcing groove.

[0010] As a further description of the above technical solution: The flow-through component includes a protective layer, the outer wall of which is fixedly connected to the inner wall of the body, and a sliding wall layer is fixedly connected to the inner wall of the protective layer.

[0011] As a further description of the above technical solution: The outer wall of the body is fixedly connected to a limiting edge, and the outer wall of the body is fixedly connected to multiple friction strips.

[0012] As a further description of the above technical solution: The outer wall of the second leaf has a sealing groove, and a sealing strip is fixedly connected to the outer wall of the second leaf.

[0013] This utility model has the following beneficial effects: 1. In this utility model, the fixing ring and the fixing groove of the leaf petal cooperate to reinforce and seal the cone and the leaf petal. The sealing groove and sealing strip of the leaf petal enhance the sealing effect. The rotating component assists the movement of the leaf petal. The reinforcing ring and reinforcing groove of the fixing component fix the leaf petal to prevent loosening and leakage of test liquid. The double leaf petal design makes it easy to adapt to different cones. The entire mechanism can change the cone according to the concentration and type of test liquid, improving the flexibility of use.

[0014] 2. In this utility model, the anti-wall-sticking mechanism includes a filter screen, filter cotton, gradient patterns, spiral channels, and flow-enhancing components. The first filter screen intercepts fine particles, the second filter screen blocks particles that may leak through the screen, the filter cotton filters aerosols and particles, preventing cross-contamination of samples and protecting the pipette. The gradient patterns guide the liquid to form a continuous liquid column, reducing liquid film separation. The spiral channels reduce the contact between the liquid and the pipe wall. The sliding wall layer of the flow-enhancing components assists in the flow of liquid, thus preventing liquid from sticking to the wall and causing contamination. Attached Figure Description

[0015] Figure 1 This is a perspective view of a filter cartridge tip for an immune repertoire assay proposed in this utility model; Figure 2 This is a front view of a filter cartridge tip for an immune repertoire assay proposed in this utility model; Figure 3 This is a cross-sectional view of the conical portion of a filter cartridge tip for an immune repertoire assay proposed in this utility model; Figure 4 This is a schematic diagram of the body of a filter cartridge tip for an immune repertoire test proposed in this utility model; Figure 5 This is a cross-sectional view of the body of a filter cartridge tip for an immune repertoire test proposed in this utility model; Figure 6 This is a cross-sectional view of a filter cartridge tip for an immune repertoire test proposed in this utility model.

[0016] Legend: 1. Conical section; 2. Body section; 3. Replacement mechanism; 301. Fixing ring one; 302. Fixing ring two; 303. Fixing groove; 304. Sealing strip; 305. Sealing groove; 306. First leaf; 307. Second leaf; 308. Rotating assembly; 3081. First rotating shaft; 3082. Second rotating shaft; 309. Fixing assembly; 3091. Reinforcing ring; 3092. Reinforcing groove; 4. Anti-wall-hanging mechanism; 401. First filter screen; 402. First engaging groove; 403. Second engaging groove; 404. Second filter screen; 405. Filter cotton; 406. Gradient pattern; 407. Spiral channel; 408. Flow-promoting assembly; 4081. Protective layer; 4082. Sliding wall layer; 5. Limiting edge; 6. Friction strip. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Reference Figure 1-4 An embodiment of this utility model is provided: a filter cartridge tip for an immune repertoire test, including a cone 1, which provides precise liquid guidance to ensure accurate aspiration and dispensing of small volumes of liquid. The outer wall of the cone 1 is provided with a replacement mechanism 3. The bottom of the outer wall of the cone 1 is fixedly connected to a body 2, which serves as the main container for aspirating, transferring and dispensing liquid samples. The inner wall of the body 2 is provided with an anti-stubble mechanism 4. The replacement mechanism 3 includes a first fixing ring 301, the inner wall of which is fixedly connected to the outer wall of the cone 1. A second fixing ring 302 is fixedly connected to the outer wall of the cone 1. A first leaf 306 is fixedly connected to the bottom of the outer wall of the cone 1. A fixing groove 303 is formed on the outer wall of the first leaf 306. The fixing ring 301 and the second fixing ring 302 are used to fix the fixing groove 303. The three work together to reinforce and seal the cone 1, the first leaf 306, and the second leaf 307. A sealing groove 305 is formed on the outer wall of the first leaf 306. A sealing strip 304 is fixedly connected to the outer wall of the first leaf 306. A second leaf 307 is fixedly connected to the outer wall of the first leaf 306. The first leaf 306 and the second leaf 307 facilitate the installation of different types of cones 1. The outer wall is provided with a rotating assembly 308, which includes a first rotating shaft 3081. The outer wall of the first rotating shaft 3081 is fixedly connected to the outer wall of the first leaf 306. The outer wall of the second leaf 307 is fixedly connected to a second rotating shaft 3082. The outer wall of the first leaf 306 is provided with a fixing assembly 309, which includes a reinforcing ring 3091. The outer wall of the reinforcing ring 3091 is fixedly connected to the outer wall of the first leaf 306. The outer wall of the first leaf 306 is provided with a reinforcing groove 3092. The reinforcing ring 3091 and the reinforcing groove 3092 are used to fix the first leaf 306 and the second leaf 307 together to prevent them from loosening and causing the test liquid to leak out. The entire mechanism realizes the replacement of the cone 1 of different sizes according to different concentrations and types of test liquids, improving its flexibility. Specifically, fixing ring 301 and fixing ring 302 work together to fix fixing groove 303. The three work together to reinforce and seal the cone 1, the first leaf 306, and the second leaf 307. The outer wall of the first leaf 306 also has a sealing groove 305, and a sealing strip 304 is fixedly connected thereto. Simultaneously, the outer wall of the first leaf 306 is connected to the second leaf 307. The design of the first leaf 306 and the second leaf 307 facilitates the installation of different types of cones 1. The outer wall of the first leaf 306 is provided with a rotating assembly 308, which includes a first rotating shaft 3081, whose outer wall is fixed to the outer wall of the first leaf 306. The second leaf... The outer wall of 307 is fixedly connected to the second rotating shaft 3082. In addition, the outer wall of the first leaf 306 is also equipped with a fixing component 309, which includes a reinforcing ring 3091. Its outer wall is fixed to the outer wall of the first leaf 306. The outer wall of the first leaf 306 is also provided with a reinforcing groove 3092. The reinforcing ring 3091 and the reinforcing groove 3092 cooperate with each other to tightly fix the first leaf 306 and the second leaf 307, preventing leakage of test liquid due to loosening. The entire replacement mechanism 3 realizes the flexible replacement of the cone 1 of the corresponding size according to different concentrations and types of test liquids through the above structure, thereby significantly improving the adaptability and operational flexibility of use.

[0019] Reference Figure 1-6 The anti-fouling mechanism 4 includes a first filter screen 401, which serves as the first stage for intercepting fine particles. The outer wall of the first filter screen 401 is fixedly connected to the inner wall of the body 2. The inner wall of the body 2 has a first engaging groove 402 and a second engaging groove 403. A second filter screen 404 is fixedly connected to the inner wall of the body 2 to block particles not intercepted by the pre-filter. The inner wall of the body 2 has gradient patterns 406, which guide the liquid flow to form a continuous liquid column, reducing liquid film separation. The inner wall of the body 2 has spiral channels 407, which guide the liquid flow during removal. The liquid flows along a spiral path, reducing the contact area between the liquid and the pipe wall. The anti-wall-hanging mechanism 4 also includes a filter cotton 405, which filters aerosols and particles to prevent cross-contamination of samples and protect the inside of the pipette. The outer wall of the filter cotton 405 is fixedly connected to the inner wall of the body 2. The inner wall of the body 2 is provided with a flow-facilitating component 408. The flow-facilitating component 408 includes a protective layer 4081. The outer wall of the protective layer 4081 is fixedly connected to the inner wall of the body 2. The inner wall of the protective layer 4081 is fixedly connected to a sliding wall layer 4082, which is coated with medical-grade perfluoropolyether (PFPE). The entire structure avoids liquid from hanging on the wall and thus preventing contamination. Specifically, the first filter 401 acts as a preliminary barrier to trap fine particles. The outer wall of the first filter 401 is fixedly connected to the inner wall of the body 2. The inner wall of the body 2 is sequentially provided with a first engaging groove 402 and a second engaging groove 403. The inner wall of the body 2 is also fixedly connected to a second filter 404, which functions to capture residual particles that the aforementioned filters failed to intercept. The inner wall of the body 2 is also processed with gradient patterns 406, which guide the liquid to form a continuous liquid column through its gradient flow channel design, effectively reducing liquid film separation. The inner wall of the body 2 is also provided with spiral channels 40. 7. During liquid transfer, it guides the liquid along a spiral trajectory, significantly reducing the contact area between the liquid and the tube wall. The filter cotton 405 is used to filter aerosols and particulate matter, preventing cross-contamination between samples and protecting the internal components of the pipette. The outer wall of the filter cotton 405 is fixed to the inner wall of the body 2, and the protective layer 4081 is fixed to the inner wall of the body 2. The inner wall of the protective layer 4081 is connected to the sliding wall layer 4082, which adopts a medical-grade perfluoropolyether (PFPE) coating. The synergistic effect of the entire structure effectively avoids liquid adhesion to the wall, thereby eliminating the risk of contamination caused by it.

[0020] Reference Figure 1-3 The outer wall of the body 2 is fixedly connected to a limiting edge 5, which is used to limit the top edge of the body 2 to prevent excessive displacement from affecting normal use. The outer wall is fixedly connected to multiple friction strips 6, which make it easy for the user to hold and fix it in a designated position. The outer wall of the second page 307 is provided with a sealing groove 305, and the outer wall of the second page 307 is fixedly connected to a sealing strip 304. Specifically, the limiting edge 5 is used to limit the top edge to prevent excessive displacement from affecting use. Its outer wall is also provided with multiple friction strips 6, which are arranged at equal intervals around the body 2 for easy gripping and fixing by the user.

[0021] Working principle: First, the fixing ring 301 and fixing ring 302 in the replacement mechanism 3 cooperate with the fixing groove 303 on the same leaf to reinforce and seal the cone 1 and the first leaf 306 and the second leaf 307. The sealing groove 305 opened on the leaf and the connecting sealing strip 304 further improve the overall sealing performance. The rotating component 308 assists the leaf to perform necessary movements through the first rotating shaft 3081 and the second rotating shaft 3082. The fixing component 309 fixes the leaf stably with the reinforcement ring 3091 and the reinforcement groove 3092, effectively preventing leakage of test liquid due to loose connection. The double leaf structure design makes it easy to adapt to the installation of cone 1 of different specifications. The entire replacement mechanism 3 can flexibly replace the cone 1 of the corresponding size according to the specific concentration and type of test liquid, which significantly improves the actual use adaptability and operation flexibility of the device. Furthermore, the anti-wall-sticking mechanism 4 includes a first filter screen 401 and a second filter screen 404, as well as filter cotton 405, gradient patterns 406, spiral channels 407, and a flow-enhancing component 408. The first filter screen 401 is responsible for intercepting fine particles in the liquid, while the second filter screen 404 further blocks particles that cannot be captured by the pre-filter. The filter cotton 405 is used to effectively filter aerosols and finer particles, preventing cross-contamination between samples and protecting the internal structure of the pipette. The gradient patterns 406 guide the liquid to form a continuous liquid column through its special flow channel, thereby reducing liquid film separation. The spiral channels 407 cause the liquid to move along a spiral trajectory during transfer, reducing the contact area with the pipette wall. The sliding wall layer 4082 in the flow-enhancing component 408 adopts a low-adhesion coating to help the liquid flow smoothly. The entire mechanism works together to fundamentally avoid liquid residue sticking to the wall and the resulting contamination problems.

[0022] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A filter cartridge tip for an immune repertoire assay, comprising a cone portion (1), characterized in that: The outer wall of the cone (1) is provided with a replacement mechanism (3), the bottom of the outer wall of the cone (1) is fixedly connected to the body (2), and the inner wall of the body (2) is provided with an anti-snagging mechanism (4). The replacement mechanism (3) includes a first fixing ring (301), the inner wall of which is fixedly connected to the outer wall of the cone (1), a second fixing ring (302) fixedly connected to the outer wall of the cone (1), a first leaf (306) fixedly connected to the bottom of the outer wall of the cone (1), a fixing groove (303) provided on the outer wall of the first leaf (306), a sealing groove (305) provided on the outer wall of the first leaf (306), a sealing strip (304) fixedly connected to the outer wall of the first leaf (306), a second leaf (307) fixedly connected to the outer wall of the first leaf (306), a rotating component (308) provided on the outer wall of the first leaf (306), and a fixing component (309) provided on the outer wall of the first leaf (306).

2. The filter tip for an immune repertoire assay according to claim 1, characterized in that: The anti-wall-hanging mechanism (4) includes a first filter screen (401), the outer wall of the first filter screen (401) is fixedly connected to the inner wall of the body (2), the inner wall of the body (2) is provided with a first engaging groove (402), the inner wall of the body (2) is provided with a second engaging groove (403), the inner wall of the body (2) is fixedly connected with a second filter screen (404), the inner wall of the body (2) is provided with gradient patterns (406), the inner wall of the body (2) is provided with a spiral channel (407), and the inner wall of the body (2) is provided with a flow-facilitating component (408).

3. The filter tip for an immune repertoire assay according to claim 2, characterized in that: The anti-wall-hanging mechanism (4) also includes a filter cotton (405), the outer wall of which is fixedly connected to the inner wall of the body (2).

4. The filter tip for an immune repertoire assay according to claim 1, characterized in that: The rotating assembly (308) includes a first rotating shaft (3081), the outer wall of which is fixedly connected to the outer wall of the first leaf (306), and the outer wall of the second leaf (307) is fixedly connected to a second rotating shaft (3082).

5. The filter tip for an immune repertoire assay according to claim 1, characterized in that: The fixing component (309) includes a reinforcing ring (3091), the outer wall of which is fixedly connected to the outer wall of the first leaf petal (306), and the outer wall of the first leaf petal (306) is provided with a reinforcing groove (3092).

6. The filter tip for an immune repertoire test according to claim 2, characterized in that: The flow-through component (408) includes a protective layer (4081), the outer wall of which is fixedly connected to the inner wall of the body (2), and a sliding wall layer (4082) is fixedly connected to the inner wall of the protective layer (4081).

7. The filter tip for an immune repertoire assay according to claim 1, characterized in that: The outer wall of the body (2) is fixedly connected to a limiting edge (5), and the outer wall of the body (2) is fixedly connected to a plurality of friction strips (6).

8. The filter tip for an immune repertoire assay according to claim 1, characterized in that: The outer wall of the second leaf (307) is provided with a sealing groove (305), and a sealing strip (304) is fixedly connected to the outer wall of the second leaf (307).

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