A glue making and punching device for hemagglutinin content detection

Abstract

This utility model relates to a gel preparation and punching device for detecting hemagglutinin content. It includes a shell, a push-pull assembly, a gel preparation assembly, and a punching assembly. The shell has a sealed cavity and a push-pull opening, which communicates with the sealed cavity. The push-pull assembly includes a push-pull box with a storage cavity at its upper opening. The push-pull box is slidably positioned at the push-pull opening along a first direction to enter the sealed cavity and close or exit the sealed cavity. The gel preparation assembly includes a gel preparation table for coagulating dissolved agar with added antibody reference material to form a gel plate body. The gel preparation table is located within the storage cavity. The punching assembly includes a puncher that moves along a second direction within the sealed cavity, allowing the puncher to enter the storage cavity through the upper opening and form detection holes in the gel plate body. The first and second directions are perpendicular. Both gel preparation and punching are completed within the sealed cavity, reducing the likelihood of impurities, dust, and other substances contaminating the gel plate, thus ensuring the accuracy of the test results.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a glue-making and punching device for detecting hemagglutinin content. Background Technology

[0002] Hemagglutinin is a receptor-binding and membrane fusion glycoprotein of the influenza virus, possessing immunogenicity; therefore, hemagglutinin antigen is an important component of influenza vaccines. The hemagglutinin content directly affects the quality of influenza virus split vaccines; therefore, the detection of hemagglutinin content is a crucial step in the testing of influenza virus split vaccines.

[0003] Generally, hemagglutinin content detection uses a one-way immunodiffusion method. A gel plate containing specific antibodies is formed by punching holes in it. A quantitative amount of antigen reference is added to the wells of the gel plate. The antigen and antibody reference specifically bind to form a precipitation ring. The diameter or area of ​​the precipitation ring is positively correlated with the concentration of the antigen reference. The hemagglutinin content of the unknown sample is then obtained based on the concentration of the antigen reference. Therefore, the quality of the gel plate used for hemagglutinin content detection directly affects the accuracy of the detection.

[0004] In existing technology, dissolved agarose containing antigen reference is first spread evenly on a gel-forming device and allowed to solidify into a gel, then holes are punched in the gel. Punching can be done using a punching device or directly by the lab technician using a hand-held puncher. Any agarose residue formed during punching can be manually removed using a pipette tip, thus completing the preparation of the gel plate.

[0005] During the gel preparation process, it is impossible to ensure that the agar gel is spread evenly on the gel preparation device, and it is also impossible to monitor the thickness of the solidified agar gel. This may result in uneven thickness of the solidified agar gel, which in turn affects the accuracy of hemagglutinin content detection.

[0006] During the drilling process, manual drilling may result in skewed holes, and manually removing the agar gum residue formed during drilling may result in incomplete removal of the gum, which in turn affects the accuracy of hemagglutinin content detection.

[0007] During the transfer of agar gel from the gel preparation device to the punching device, impurities, dust, and other substances may fall onto the gel plate, causing contamination. These substances may react non-specifically with the components in the gel plate or the unknown sample to be tested, or change the chemical environment of the reaction system, thereby interfering with the specific binding of hemagglutinin antigens in the unknown sample to the corresponding antibodies or receptors added to the gel plate, leading to inaccurate test results.

[0008] Therefore, there is an urgent need for a gel preparation and punching device for detecting hemagglutinin content to solve the above-mentioned technical problems. Utility Model Content

[0009] The purpose of this invention is to provide a glue-making and punching device for detecting hemagglutinin content, so as to solve at least one of the above-mentioned problems.

[0010] To achieve the above objectives, this utility model provides a gel-making and punching device for detecting hemagglutinin content, comprising:

[0011] The outer casing has a sealed cavity and a push-pull opening, the push-pull opening being able to communicate with the sealed cavity;

[0012] A push-pull assembly includes a push-pull box having a storage cavity with an opening at the upper end. The push-pull box is disposed at the push-pull opening in a first direction to enter the sealed cavity and close the push-pull opening or exit the sealed cavity.

[0013] A gel-forming assembly includes a gel-forming station for coagulating dissolved agar gel containing antibody reference material to form a gel plate body, the gel-forming station being disposed within the storage cavity;

[0014] A punching assembly includes a punch that is movable in a second direction within the sealed cavity, such that the punch enters the storage cavity through the upper opening of the storage cavity and forms a detection hole on the adhesive plate body, wherein the first direction and the second direction are perpendicular to each other.

[0015] Furthermore, the glue-making and punching device for detecting hemagglutinin content also includes a thickness gauge for measuring the thickness of the solidified glue sheet body, the thickness gauge being installed inside the sealed cavity.

[0016] Furthermore, the adhesive-making assembly also includes a moving component, which can move the adhesive-making table along a third direction within the storage cavity, so that the adhesive-making table is directly opposite the thickness gauge or the punch, and the third direction, the first direction, and the second direction are perpendicular to each other.

[0017] Furthermore, the punching assembly also includes a punching plate and an electric push rod. The electric push rod is installed on the housing, and its output end extends into the sealed cavity and is connected to the punching plate. The punch is disposed on the punching plate. The output end of the electric push rod extends and retracts along the second direction to drive the punch into the storage cavity through the upper opening of the storage cavity. The first direction and the second direction are perpendicular to each other.

[0018] Furthermore, the glue-making and punching device for detecting hemagglutinin content also includes a damping component, which is disposed on the punching plate and is used to buffer the thrust of the electric push rod.

[0019] Furthermore, the damping assembly includes a contact rod, a fixed rod, and a damping spring. The first end of the contact rod can contact the glue-making table, the fixed rod has a telescopic cavity, the second end of the contact rod is telescopically disposed in the telescopic cavity, and the damping spring is connected to the cavity wall of the telescopic cavity and the second end of the contact rod, respectively.

[0020] Furthermore, the gel-making and perforation device for detecting hemagglutinin content also includes an adsorption component, which includes a vacuum pump and a vacuum chamber. The perforator opens the adsorption chamber, and the perforation plate has adsorption holes. The adsorption chamber can communicate with the adsorption holes, and the vacuum chamber is connected to both the adsorption holes and the vacuum pump to draw out the residue formed by perforation from the detection hole.

[0021] Furthermore, the punch includes a filter screen disposed inside the adsorption chamber to prevent residues formed during punching from entering the vacuum chamber.

[0022] Furthermore, the perforated plate has a threaded hole communicating with the adsorption hole, and the punch includes an integrally formed punching part and a threaded part. The punching part is used to punch holes in the rubber sheet body, and the threaded part is threadedly connected to the threaded hole.

[0023] Furthermore, the gel-making assembly also includes a gel-making plate and a positioning element. The gel-making table has a gel-making groove, and the positioning element can position the gel-making plate in the gel-making groove. The gel-making plate is used to solidify the dissolved agar gel containing antibody reference material.

[0024] Furthermore, the push-pull assembly also includes a guide assembly, which includes a guide block, a guide rail, and a buffer assembly. The guide rail extends along a first direction and is disposed on the cavity wall of the sealed cavity. The guide block is mounted on the push-pull box and is slidably disposed within the guide rail. The buffer assembly is disposed on the end of the guide rail away from the push-pull opening and is capable of abutting against the push-pull box.

[0025] The beneficial effects of this utility model are as follows:

[0026] The present invention provides a gel preparation and punching device for detecting hemagglutinin content, comprising a shell, a push-pull assembly, a gel preparation assembly, and a punching assembly. The shell has a sealed cavity and a push-pull opening, which communicates with the sealed cavity. The push-pull assembly includes a push-pull box with a storage cavity and an opening at the top. The push-pull box is disposed at the push-pull opening in a first direction to enter the sealed cavity and close or exit the sealed cavity. The gel preparation assembly includes a gel preparation table for coagulating dissolved agar with added antibody reference to form a gel plate body. The gel preparation table is disposed within the storage cavity. The punching assembly includes a puncher that moves in a second direction within the sealed cavity so that the puncher enters the storage cavity through the opening at the top and forms detection holes on the gel plate body. The first and second directions are perpendicular to each other. Pull the sliding box out from the sliding opening in the first direction, then lay the dissolved agarose gel containing antibody reference on the gel preparation table, push the sliding box back and close the sliding opening. After the dissolved agarose gel containing antibody reference solidifies on the gel preparation table to form the gel plate body, start the punch and move it in the second direction to make the punch form detection holes on the gel plate body. After gel preparation and punching, the gel plate for detecting hemagglutinin content is prepared. The sliding box allows for the dissolving agar gel containing antibody reference material to be laid on the gel preparation platform. When the sliding box is closed, the sealed chamber is isolated from the outside environment. Gel preparation and perforation are both completed within the sealed chamber, reducing the chance of impurities, dust, and other substances contaminating the gel plate. This also reduces the likelihood of non-specific reactions between these substances and the components in the gel plate or the unknown sample to be tested, as well as the chance of these substances altering the chemical environment of the reaction system. Furthermore, it reduces interference from the specific binding of hemagglutinin antigens in the unknown sample to the corresponding antibodies or receptors added to the gel plate, thereby ensuring the accuracy of the test results. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the adhesive preparation and punching device for detecting hemagglutinin content provided in this embodiment of the utility model;

[0028] Figure 2 This is a cross-sectional view of the gel-making and punching device for detecting hemagglutinin content provided in this embodiment of the utility model;

[0029] Figure 3 This is a top view of the push-pull box and glue-making component structure provided in this embodiment of the utility model;

[0030] Figure 4 This is a schematic diagram of the structure of the damping component provided in this embodiment of the utility model;

[0031] Figure 5 This is a schematic diagram of the structure of the hole punch provided in this embodiment of the utility model;

[0032] Figure 6This is a cross-sectional view of the perforated plate provided in this embodiment of the utility model;

[0033] Figure 7 This is a cross-sectional view of the guide rail provided in an embodiment of this utility model.

[0034] In the picture:

[0035] 1. Outer shell; 11. Sealed cavity;

[0036] 2. Sliding assembly; 21. Sliding box; 211. Storage cavity; 22. Guide assembly; 221. Guide block; 222. Guide rail; 2221. Guide groove; 223. Buffer; 224. Buffer spring; 225. Fixing plate; 23. Handle;

[0037] 3. Glue-making assembly; 31. Glue-making table; 311. Glue-making tank; 32. Moving assembly; 321. Pusher; 322. Pulley; 323. Slide rail; 33. Temperature control; 34. Glue-making plate; 35. Positioning component;

[0038] 4. Drilling assembly; 41. Driller; 411. Drilling part; 412. Threaded part; 413. Filter screen; 414. Adsorption chamber; 42. Drilling plate; 421. Adsorption hole; 422. Threaded hole; 43. Electric push rod;

[0039] 5. Damping assembly; 51. Contact rod; 52. Fixing rod; 521. Telescopic cavity; 53. Damping spring;

[0040] 6. Adsorption assembly; 61. Vacuum pump; 62. Enclosure; 621. Connection port; 63. Vacuum chamber; 64. Connecting pipe; 65. Push base;

[0041] 7. Thickness gauge;

[0042] 8. Leveling assembly; 81. Leveling support leg; 811. Support body; 812. Threaded rod; 813. Nut; 814. Connecting rod; 82. Level;

[0043] 9. Display screen;

[0044] 10. Operation screen. Detailed Implementation

[0045] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this utility model and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this utility model are shown in the accompanying drawings, not all of them.

[0046] This utility model defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up", "down", "left", "right", "inner", and "outer", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this utility model.

[0047] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0048] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0049] Example 1

[0050] like Figures 1-7As shown, this embodiment provides a glue-making and punching device for detecting hemagglutinin content. The device includes a housing 1, a push-pull assembly 2, a glue-making assembly 3, and a punching assembly 4. The housing 1 has a sealed cavity 11 and a push-pull opening, which communicates with the sealed cavity 11. The push-pull assembly 2 includes a push-pull box 21 with a storage cavity 211. The upper end of the storage cavity 211 is open, and the push-pull box 21 is slidably disposed at the push-pull opening along a first direction. The agar gel assembly 3 includes a gel preparation table 31, which is used to coagulate dissolved agar gel containing antibody reference material to form a gel plate body. The gel preparation table 31 is located in the storage cavity 211. The punching assembly 4 includes a punch 41, which is moved along the second direction and located in the sealed cavity 11 so that the punch 41 enters the storage cavity 211 through the upper opening of the storage cavity 211 and forms detection holes on the gel plate body. Pull the sliding box 21 out from the sliding opening along the first direction, then lay the dissolved agar gel containing antibody reference on the gel preparation table 31, push the sliding box 21 back and close the sliding opening. After the dissolved agar gel containing antibody reference solidifies on the gel preparation table 31 to form the gel plate body, start the punch 41 to move along the second direction so that the punch 41 forms detection holes on the gel plate body. After gel preparation and punching, the gel plate for detecting hemagglutinin content is prepared. By pulling out the sliding box 21, dissolved agarose gel containing antibody reference material is laid on the gel preparation table 31. When the sliding box 21 is closed, the sealed cavity 11 is isolated from the outside. Gel preparation and perforation are both completed within the sealed cavity 11, reducing the probability of impurities, dust, and other substances contaminating the gel plate. This also reduces the probability of non-specific reactions between these substances and the components in the gel plate or the unknown sample to be tested, as well as the probability of these substances altering the chemical environment of the reaction system. Furthermore, it reduces interference from the specific binding of hemagglutinin antigens in the unknown sample to the corresponding antibodies or receptors added to the gel plate, thereby ensuring the accuracy of the test results.

[0051] like Figure 1 As shown, the push-pull assembly 2 also includes a handle 23, which is disposed on the push-pull box 21 to push and pull the push-pull box 21.

[0052] The agar-agar preparation and punching device for detecting hemagglutinin content also includes a leveling assembly 8. The leveling assembly 8 includes leveling feet 81 and a level 82. Multiple leveling feet 81 are located on the bottom surface of the outer casing 1 and supported on the ground. The height of the leveling feet 81 is adjustable. The level 82 is located on the side of the outer casing 1 and can detect whether the current plane is level. By adjusting the height of each leveling foot 81 and observing whether the level 82 is level, the sliding box 21 inside the outer casing 1 is ensured to be on a level surface, thereby ensuring that the agar-agar preparation table 31 is on a level surface, thus ensuring uniform thickness when the agar-agar solidifies to form the glue sheet body.

[0053] The leveling support 81 includes a support body 811, a threaded rod 812, a nut 813, and a connecting rod 814. The support body 811 is supported on the ground and is connected to the threaded rod 812. The connecting rod 814 is connected to the nut 813 and the bottom surface of the outer casing 1, respectively. The threaded rod 812 is threadedly connected to the nut 813. By rotating the support body 811 and the threaded rod 812, the threaded connection on the threaded rod 812 and the nut 813 is moved to different positions, thereby achieving the purpose of fine-tuning the height of the leveling support 81. This adjusts the distance between the bottom surface of the outer casing 1 and the ground, so as to level the glue-making table 31 and ensure that the thickness of the agar gel solidifies into a uniform glue sheet body.

[0054] In this embodiment, the height of the leveling support 81 refers to the dimension of the leveling support 81 along the second direction.

[0055] In this embodiment, there are four leveling feet 81, which are located at the four corners of the bottom surface of the outer casing 1.

[0056] In this embodiment, the leveling support 81 is made of stainless steel. The support body 811 is circular with a diameter of 2cm, and the maximum dimension of the leveling support 81 along the second direction is 3cm.

[0057] There are four levels 82. Each side of the outer casing 1 is equipped with a level 82 to detect whether the outer casing 1, the push-pull box 21 and the glue-making table 31 are in a horizontal state after being leveled by the leveling support legs 81.

[0058] In this embodiment, the level 82 is a bubble level, specifically the SCREW-ON SPIPITLEVEL 66. Bubble levels are simple in structure, inexpensive, easy to use, require no power supply, and allow users to determine the levelness directly by observing the bubble position.

[0059] The gel preparation and punching device for detecting hemagglutinin content also includes a display screen 9, which is located on the side of the housing 1 where the sliding opening is opened. The display screen 9 is used to display information such as the curing time of agar, the temperature of the gel preparation table 31, and the thickness of the gel plate body.

[0060] The glue-making and punching device for detecting hemagglutinin content also includes an operation screen 10. The operation screen 10 is located on the side of the housing 1 with a push-pull opening. The operation screen 10 is used to control the temperature of the glue-making table 31, open and close the punch 41, and remove residues from the detection holes.

[0061] In this embodiment, both the outer shell 1 and the push-pull box 21 are made of acrylic material.

[0062] In this embodiment, the panel of the sliding box 21 with the sliding opening closed is provided with transparent tempered glass so that the preparation of the rubber sheet inside the sealed cavity 11 can be observed at any time through the tempered glass.

[0063] like Figure 2 As shown, the gel-making and punching apparatus for detecting hemagglutinin content also includes a thickness gauge 7 for detecting the thickness of the solidified gel sheet body. The thickness gauge 7 is installed inside the sealed cavity 11. The thickness of the solidified agar gel can be detected by the thickness gauge 7 to determine whether the thickness of the gel sheet body is uniform.

[0064] The adhesive application assembly 3 also includes a moving assembly 32, which can move the adhesive application table 31 along a third direction into the storage cavity 211, so that the adhesive application table 31 is directly opposite the thickness gauge 7 or the punch 41, with the third direction, the first direction, and the second direction being perpendicular to each other. The moving assembly 32 moves the adhesive application table 31 so that it is directly opposite the thickness gauge 7 or the punch 41.

[0065] Before operation, the gel preparation and punching device for hemagglutinin content detection is configured such that the gel preparation platform 31 is aligned with the thickness gauge 7. The push-pull box 21 is pulled out of its opening to spread the dissolved agar gel containing antibody reference material evenly onto the gel preparation platform 31. The push-pull box 21 is then pushed back into its opening to close it. The solidified agar gel can be used to check the thickness of the gel plate body using the thickness gauge 7 to determine if the thickness of the gel plate body is uniform. If the thickness is uniform, the gel preparation platform 31 and the gel plate body are moved to be aligned with the punch 41 using the moving component 32. The punch 41 punches holes in the gel plate body to form detection holes. If the thickness is uneven, the gel plate body solidified this time needs to be discarded, and the agar gel needs to be prepared again and solidified to form the gel plate body again.

[0066] In this embodiment, the thickness gauge 7 is an infrared thickness gauge, and the model of the infrared thickness gauge is the IRMD series of the Chino brand.

[0067] In this embodiment, the first direction refers to Figures 1-3 In the Y direction, the second direction refers to Figures 1-3 In the Z direction, the third direction refers to Figures 1-3 The X direction. When the leveling component 8 adjusts the outer casing 1 to a horizontal position, the Z direction is the vertical direction, and the first and third directions are horizontal.

[0068] In this embodiment, the outer shell 1 has a dimension of 40cm along the third direction, a dimension of 20cm along the first direction, a dimension of 34cm along the second direction, and a thickness of 2cm.

[0069] In this embodiment, the push-pull box 21 has a dimension of 30cm along the third direction, a dimension of 18cm along the first direction, a dimension of 16cm along the second direction, and a thickness of 1.5cm.

[0070] In this embodiment, both the display screen 9 and the operation screen 10 are LCDs (Liquid Crystal Displays), with a dimension of 15cm along the third direction and a dimension of 4cm along the first direction.

[0071] The punching assembly 4 also includes a punching plate 42 and an electric push rod 43. The electric push rod 43 is mounted on the housing 1, and its output end extends into the sealed cavity 11 and is connected to the punching plate 42. The punch 41 is disposed on the punching plate 42. The output end of the electric push rod 43 extends and retracts along the second direction to drive the punch 41 into the storage cavity 211 through the upper opening of the storage cavity 211 and form a detection hole on the adhesive plate body. The first direction and the second direction are perpendicular to each other. The electric push rod 43 drives the punching plate 42 to move along the second direction, thereby driving the punch 41 to move along the second direction to form a detection hole on the adhesive plate body.

[0072] The electric push rod 43 is installed on the top surface of the housing 1. After being leveled by the leveling component 8, the top surface of the housing 1 is also on the horizontal plane, thereby ensuring that the output end of the electric push rod 43 extends along the second direction, thereby ensuring that the punch 41 is vertical and the hole diameter is uniform in the detection hole formed on the rubber plate body, and that the formed detection hole is not skewed, thereby ensuring the accuracy of hemagglutinin content detection.

[0073] The operation panel 10 is used to control the opening and closing of the electric push rod 43, thereby controlling the opening and closing of the punch 41.

[0074] The agar gel preparation and punching device for hemagglutinin content detection also includes an adsorption component 6, which is used to extract the residue formed by punching from the detection holes. The adsorption component 6 includes a vacuum pump 61 and a vacuum chamber 63. The puncher 41 opens an adsorption chamber 414, and the punching plate 42 has adsorption holes 421. The adsorption chamber 414 and the adsorption holes 421 are connected. The vacuum chamber 63 is connected to both the adsorption holes 421 and the vacuum pump 61 to extract the residue formed by punching from the detection holes. The operation panel 10 can control the opening and closing of the vacuum pump 61. When the vacuum pump 61 is started, it generates adsorption force, which can draw the residue formed by punching into the adsorption chamber 414. Compared to manually picking out the agar gel residue formed by punching, the adsorption component 6 adsorbs the residue more thoroughly, leaving no residue in the detection holes, thus ensuring the accuracy of hemagglutinin content detection.

[0075] The adsorption assembly 6 also includes a pusher 65 and a surrounding plate 62. The output end of the electric thruster 321 extends into the sealed cavity 11 and is connected to the pusher 65. The surrounding plate 62 is disposed on the pusher 65, and the perforated plate 42 is disposed on the surrounding plate 62. The pusher 65, the surrounding plate 62 and the perforated plate 42 form the aforementioned vacuum cavity 63.

[0076] The adsorption assembly 6 also includes a connecting tube 64, one end of which is connected to the vacuum pump 61 and the other end of which is connected to the vacuum chamber 63.

[0077] A connection port 621 is provided on the enclosure 62, which is connected to the vacuum chamber 63, and the end of the connecting pipe 64 is connected to the connection port 621.

[0078] like Figure 2 and Figure 3 As shown, the moving component 32 includes a thruster 321, which is disposed on the glue-making stage 31 and can drive the glue-making stage 31 to move in a third direction within the storage cavity 211.

[0079] The control panel 10 can control the opening and closing of the pusher 321, thereby controlling the movement of the glue-making table 31.

[0080] The movable component 32 also includes a pulley 322 and a slide rail 323. The pulley 322 is located on the bottom surface of the glue-making table 31, and the slide rail 323 is located in the storage cavity 211. The slide rail 323 extends in a third direction, and the pulley 322 is guided by the slide rail 323.

[0081] In this embodiment, there are two slide rails 323, which are arranged in parallel and spaced apart in the storage cavity 211 along the first direction, so as to guide the movement of the glue-making table 31.

[0082] In this embodiment, the diameter of pulley 322 is 0.8cm and the dimension along the first direction is 1.5cm. The dimension of slide rail 323 along the first direction is 2cm and the dimension along the third direction is 27cm.

[0083] In this embodiment, both the slide rail 323 and the pulley 322 are made of stainless steel.

[0084] The gel casting assembly 3 also includes a gel casting plate 34. The gel casting table 31 has a gel casting tank 311, and the gel casting plate 34 is positioned within the tank. The gel casting plate 34 is used to solidify the dissolved agar gel containing antibody reference material. The dissolved agar gel containing antibody reference material is spread evenly on the gel casting plate 34, and the agar gel does not flow out of the gel casting plate 34 due to its own surface tension. In this embodiment, the gel casting plate 34 is made of glass.

[0085] In this embodiment, the dimension of the glue-making tank 311 along the third direction is 14cm, and the dimension along the first direction is 11cm.

[0086] The agar-agar preparation assembly 3 also includes a temperature control unit 33, which is located inside the agar-agar preparation table 31 and directly opposite the agar-agar preparation tank 311 along the second direction. The temperature control unit 33 is used to control the temperature of the agar-agar preparation plate 34. The temperature control unit 33 generates heat and transfers the heat to the agar-agar preparation plate 34 in the agar-agar preparation tank 311 to achieve the purpose of controlling the temperature of the agar-agar preparation plate 34. Controlling the temperature of the agar-agar preparation plate 34 helps to spread and solidify the agar-agar evenly. If the temperature is too high, the effective components in the agar-agar will be deactivated, thereby affecting the accuracy of the hemagglutinin content detection; if the temperature is too low, the agar-agar will not be able to spread evenly on the agar-agar preparation plate 34.

[0087] In this embodiment, the temperature control unit 33 is a temperature control board, and the model of the temperature control board can be ACT-B1. The temperature control board can control the temperature of the glue-making plate 34 between 25℃ and 45℃. The temperature of the glue-making plate 34 can be controlled through the operation screen 10 and displayed on the display screen 9.

[0088] like Figure 2 and Figure 7 As shown, the push-pull assembly 2 also includes a guide assembly 22, which includes a guide block 221 and a guide rail 222. The guide rail 222 extends along a first direction and is disposed on the cavity wall of the sealed cavity 11. The guide block 221 is mounted on the push-pull box 21 and is slidably disposed within the guide rail 222. The guide block 221 and the guide rail 222 provide guidance for the push-pull movement of the push-pull box 21.

[0089] In this embodiment, the guide rail 222 has a dimension of 17.5cm along the first direction and a dimension of 2.5cm along the third direction.

[0090] In this embodiment, both the guide rail 222 and the guide block 221 are made of stainless steel.

[0091] The number of guide components 22 can be multiple, and multiple guide components 22 can be provided on the bottom or side of the push-pull box 21.

[0092] The specific usage process of the gel preparation and punching device for hemagglutinin content detection provided in this embodiment is as follows:

[0093] By adjusting the leveling feet 81 to make the outer casing 1 and the glue plate 34 level, the output end of the electric push rod 43 also extends in the second direction.

[0094] Then connect the power and turn on the machine. On the operation screen 10, adjust the temperature of the agar plate 34 to 54℃ and preheat for 10 minutes to ensure that the agar can be spread horizontally. The temperature of the agar plate 34 can be observed through the display screen 9.

[0095] After preheating, open the sliding box 21, mix the serum with 1.5% agar gel at a temperature below 56°C until homogeneous, and manually spread it evenly on the gel preparation plate 34. After the agar gel is completely spread on the gel preparation plate 34, close the sliding box 21, start timing on the display screen 9, close the temperature control 33 through the operation screen 10, and wait for the agar gel to solidify. The solidification time is more than 40 minutes. After solidification, the thickness of the gel plate can be detected by the thickness gauge 7 and displayed on the display screen 9. Temperatures above 56°C will cause protein denaturation. Since serum is added to the agar gel, temperatures above 56°C will affect the activity of the serum and affect the experiment.

[0096] When the thickness of the solidified glue sheet is uniform, the pusher 321 is controlled on the operation screen 10 to move the glue-making table 31 to face the punch 41, and the glue-making table 31 is fixed by the fastener.

[0097] The electric push rod 43 is activated on the operation screen 10, which causes the punch 41 to start punching holes. The punch 41 punches holes vertically downwards on the rubber sheet body.

[0098] After drilling is completed, the vacuum pump 61 is turned on through the operation panel 10 to draw a vacuum and suck out the residue formed by drilling from the test hole, thereby removing the residual agarose in the test hole.

[0099] Example 2

[0100] Based on Embodiment 1, the moving component 32 also includes a fixing member, which can fix the glue-making table 31 at the position of the slide rail 323 facing the punch 41, so that the glue-making table 31 will not shift when punching, thereby preventing the punching on the glue plate body from being crooked.

[0101] In this embodiment, a fixing member can be installed on the slide rail 323 at a position directly opposite the punch 41. The fixing member is retractably installed inside the slide rail 323. When the glue-making table 31 moves to a position where the slide rail 323 is directly opposite the punch 41, the fixing member extends and engages with the glue-making table 31 or the moving assembly 32. Alternatively, the fixing member can be a brake, with a brake pad installed on the pulley 322. A control button is provided on the operation screen 10, which can control whether the brake is engaged or disengaged.

[0102] Example 3

[0103] Based on any of the embodiments in Embodiment 1-2, the glue-making assembly 3 further includes a positioning member 35, which can position the glue-making plate 34 in the glue-making groove 311.

[0104] The positioning element 35 can be four vertical corner frames, which are respectively located at the four corners of the glue-making table 31. The vertical corner frames can abut against the corners of the glue-making plate 34 to restrict the movement of the glue-making plate 34 in the first direction and the third direction, that is, to fix the glue-making plate 34 in the glue-making groove 311 of the glue-making table 31.

[0105] The dimension of the positioning element 35 along the second direction is larger than that of the gel casting plate 34 along the second direction. After the dissolved agarose containing antibody reference material is laid flat on the gel casting plate 34, it solidifies to form the gel casting plate body. Along the second direction, the positioning element 35 protrudes from the gel casting plate body, and also provides some resistance to the dissolved agarose containing antibody reference material. The dissolved agarose containing antibody reference material is laid flat on the gel casting plate 34, and the agarose does not flow out of the gel casting plate 34 due to its own surface tension and the resistance of the positioning element 35.

[0106] Example 4

[0107] Based on any of the embodiments in Examples 1-3, such as Figure 2 and Figure 4 As shown, the adhesive preparation and drilling device for hemagglutinin content detection also includes a damping component 5, which is mounted on the drilling plate 42. The damping component 5 is used to buffer the thrust of the electric push rod 43, preventing excessive force from causing the drill 41 to collide with the adhesive preparation plate 34. Since the adhesive preparation plate 34 is made of glass, excessive force when the drill 41 contacts the adhesive plate body under the drive of the electric push rod 43 could cause the glass to shatter.

[0108] There are multiple damping components 5, which are arranged at intervals along the outer contour of the perforated plate 42, and all of the multiple damping components 5 can contact the glue-forming table 31. Specifically, the multiple damping components 5 can contact the area on the glue-forming table 31 that does not overlap with the glue-forming plate 34, so as to prevent the damping components 5 from contacting the glue sheet body formed by solidification on the glue-forming plate 34, and ensure that the damping components 5 will not damage the glue sheet body.

[0109] In this embodiment, the dimension of the damping component 5 along the second direction is 5cm.

[0110] In this embodiment, the damping component 5 is made of stainless steel.

[0111] The damping assembly 5 includes a contact rod 51, a fixed rod 52, and a damping spring 53. The first end of the contact rod 51 can contact the glue-making table 31. The fixed rod 52 has a telescopic cavity 521. The second end of the contact rod 51 is telescopically disposed in the telescopic cavity 521. The damping spring 53 is connected to the cavity wall of the telescopic cavity 521 and the second end of the contact rod 51, respectively. Specifically, the first end of the contact rod 51 can contact the area of ​​the glue-making table 31 that does not overlap with the glue-making plate 34.

[0112] Example 5

[0113] Based on any of the embodiments in Examples 1-4, such as Figure 2 , Figure 5 and Figure 6 As shown, the punch 41 includes a filter screen 413, which is disposed inside the adsorption chamber 414 to prevent residues formed during punching from entering the vacuum chamber 63 and becoming difficult to clean. Accordingly, the punch 41 needs to be cleaned periodically.

[0114] The perforated plate 42 has a threaded hole 422 communicating with the adsorption hole 421. The punch 41 includes an integrally formed punching part 411 and a threaded part 412. The punching part 411 is used to punch holes in the rubber sheet body, and the threaded part 412 is threadedly connected to the threaded hole 422. The threaded connection between the punch 41 and the perforated plate 42 makes it easy to remove the punch 41 from the perforated plate 42.

[0115] In this embodiment, the adsorption cavity 414 is a through hole of the through punch 41.

[0116] The perforated plate 42 has multiple threaded holes 422, each corresponding to an adsorption hole 421. These threaded holes 422 are arranged in rows and columns, and each threaded hole 422 can be connected to a punch 41. By creating multiple threaded holes 422 on the perforated plate 42, each corresponding to a punch 41, the spacing between the punches 41 is adjustable and uniform. This ensures uniform hole spacing on the adhesive plate body, thereby guaranteeing the accuracy of hemagglutinin content detection.

[0117] The punch 41 can be freely removed from the threaded hole 422. After removal, the corresponding threaded hole 422 can be plugged with a rubber plug. By selecting different positions and different numbers of threaded holes 422 to connect the punch 41, the layout of the punch 41 can be manually adjusted, thereby adjusting the layout of the detection holes on the rubber sheet body.

[0118] In this embodiment, the diameter of the punch 41 is 3mm, and the dimension along the second direction is 4cm.

[0119] In this embodiment, the punch 41 is made of stainless steel.

[0120] The dimension of the punch 41 along the second direction is smaller than the dimension of the damping component 5 along the first direction. Therefore, the damping component 5 contacts the adhesive application component 3 before the punch 41, so that the damping component 5 plays a buffering role.

[0121] Example 6

[0122] Based on any of the embodiments from Example 1 to Example 5, the perforated plate 42 and the surrounding plate 62 are detachably connected to allow for replacement with perforated plates 42 of different specifications. The different specifications of the perforated plate 42 refer to the different number of rows and columns formed by the threaded holes 422 on the perforated plate 42, that is, the different layout of the multiple threaded holes 422.

[0123] Example 7

[0124] Based on any of the embodiments from Embodiment 1 to Embodiment 6, the guide assembly further includes a buffer assembly. The buffer assembly is located on the end of the guide rail 222 away from the push-pull opening, and the buffer assembly can abut against the push-pull box 21. The buffer assembly can prevent the agar glue spread on the glue-making plate 34 from overflowing due to excessive force when pushing or pulling the push-pull box 21.

[0125] A guide groove 2221 is provided on the guide rail 222, and the buffer assembly is located in the guide groove 2221.

[0126] The buffer assembly includes a buffer element 223, a buffer spring 224, and a fixing plate 225. The fixing plate 225 is located on the end of the guide groove 2221 away from the push-pull opening. The buffer element 223 can abut against the push-pull box 21. The buffer spring 224 is connected to the fixing plate 225 and the buffer element 223 respectively.

[0127] Example 8

[0128] Based on any of the embodiments 1-7, since the measuring diameter of the infrared thickness gauge is 50mm*50mm, when the size of the glue sheet body is larger than the measuring diameter of the infrared thickness gauge, if the infrared thickness gauge and the glue sheet body do not move relative to each other, the thickness of some areas on the glue sheet body cannot be measured by the infrared thickness gauge. Therefore, it is necessary to adjust the relative position of the infrared thickness gauge and the glue sheet body so that the infrared thickness gauge can measure the thickness at any position on the glue sheet body.

[0129] After the glue sheet body solidifies, the moving component 32 can be controlled by the operation screen to move along a third direction, thereby driving the glue-making table 31 and the solidified glue sheet body on the glue-making table 31 to move along a third direction, so that the infrared thickness gauge and the glue sheet body move relative to each other in the third direction, so that the infrared thickness gauge can measure the thickness of the glue sheet body in the area beyond the measurement range in the third direction; or, the infrared thickness gauge can be movably disposed in the sealed cavity 11 along the first direction, and the relative position of the infrared thickness gauge and the glue sheet body can be adjusted by moving the infrared thickness gauge along the first direction and moving the glue-making table along the third direction, so that the infrared thickness gauge can measure the thickness at any position on the glue sheet body; or, the infrared thickness gauge can be movably disposed in the sealed cavity 11 along the first direction and the third direction, and the relative position of the infrared thickness gauge and the glue sheet body can be adjusted by moving the infrared thickness gauge along the first direction and the third direction, so that the infrared thickness gauge can measure the thickness at any position on the glue sheet body.

[0130] In this embodiment, the mechanism for moving the infrared thickness gauge is not limited, and common linear mechanisms, such as cylinder drive, can be selected.

[0131] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A gel-making and punching apparatus for detecting hemagglutinin content, characterized in that, include: The outer shell (1) has a sealed cavity (11) and a push-pull opening, the push-pull opening being able to communicate with the sealed cavity (11); A push-pull assembly (2) includes a push-pull box (21) having a storage cavity (211) with an opening at the upper end of the storage cavity (211). The push-pull box (21) is provided at the push-pull opening in a first direction to enter the sealed cavity (11) and close the push-pull opening or exit the sealed cavity (11). A gel-forming assembly (3) includes a gel-forming station (31) for coagulating dissolved agar gel containing antibody reference material to form a gel plate body, the gel-forming station (31) being disposed within the storage cavity (211). The punching assembly (4) includes a punch (41) which is moved along a second direction and disposed in the sealed cavity (11) so that the punch (41) enters the storage cavity (211) through the upper opening of the storage cavity (211) and forms a detection hole on the adhesive plate body. The first direction and the second direction are perpendicular to each other.

2. The gel preparation and punching apparatus for detecting hemagglutinin content according to claim 1, characterized in that, The glue-making and punching device for detecting hemagglutinin content also includes a thickness gauge (7) for measuring the thickness of the solidified glue sheet body, and the thickness gauge (7) is installed in the sealed cavity (11).

3. The gel preparation and punching apparatus for hemagglutinin content detection according to claim 2, characterized in that, The glue-making assembly (3) also includes a moving assembly (32), which can drive the glue-making table (31) to move along a third direction and be placed in the storage cavity (211) so that the glue-making table (31) is directly opposite the thickness gauge (7) or the punch (41), and the third direction, the first direction and the second direction are perpendicular to each other.

4. The gel preparation and punching apparatus for detecting hemagglutinin content according to claim 1, characterized in that, The punching assembly (4) further includes a punching plate (42) and an electric push rod (43). The electric push rod (43) is installed on the housing (1). The output end of the electric push rod (43) extends into the sealed cavity (11) and is connected to the punching plate (42). The punch (41) is disposed on the punching plate (42). The output end of the electric push rod (43) extends and retracts along the second direction to drive the punch (41) to enter the storage cavity (211) through the upper opening of the storage cavity (211). The first direction and the second direction are perpendicular to each other.

5. The gel preparation and punching apparatus for detecting hemagglutinin content according to claim 4, characterized in that, The glue-making and punching device for detecting hemagglutinin content also includes a damping component (5), which is disposed on the punching plate (42) and is used to buffer the thrust of the electric push rod (43).

6. The gel-making and punching apparatus for detecting hemagglutinin content according to claim 5, characterized in that, The damping assembly (5) includes a contact rod (51), a fixing rod (52), and a damping spring (53). The first end of the contact rod (51) can contact the glue-making table (31). The fixing rod (52) has a telescopic cavity (521). The second end of the contact rod (51) is telescopically located in the telescopic cavity (521). The damping spring (53) is connected to the cavity wall of the telescopic cavity (521) and the second end of the contact rod (51) respectively.

7. The gel preparation and punching apparatus for detecting hemagglutinin content according to claim 4, characterized in that, The gel-making and perforation device for detecting hemagglutinin content also includes an adsorption component (6), which includes a vacuum pump (61) and a vacuum chamber (63). The perforator (41) has an adsorption chamber (414), and the perforation plate (42) has an adsorption hole (421). The adsorption chamber (414) is connected to the adsorption hole (421). The vacuum chamber (63) is connected to the adsorption hole (421) and the vacuum pump (61) respectively, so as to draw out the residue formed by perforation from the detection hole.

8. The gel-making and punching apparatus for detecting hemagglutinin content according to claim 7, characterized in that, The punch (41) includes a filter screen (413) disposed in the adsorption chamber (414) to prevent the residue formed by punching from entering the vacuum chamber (63).

9. The gel-making and punching apparatus for detecting hemagglutinin content according to claim 7, characterized in that, The perforated plate (42) has a threaded hole (422) communicating with the adsorption hole (421). The punch (41) includes an integrally formed punching part (411) and a threaded part (412). The punching part (411) is used to punch holes in the rubber sheet body, and the threaded part (412) is threadedly connected to the threaded hole (422).

10. The gel-making and punching apparatus for detecting hemagglutinin content according to claim 1, characterized in that, The push-pull assembly (2) further includes a guide assembly (22), which includes a guide block (221), a guide rail (222), and a buffer assembly. The guide rail (222) extends along a first direction and is disposed on the cavity wall of the sealed cavity (11). The guide block (221) is mounted on the push-pull box (21) and is slidably disposed within the guide rail (222). The buffer assembly is disposed on the end of the guide rail (222) away from the push-pull opening and is able to abut against the push-pull box (21).

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