Exosome culture bioreactor

Abstract

The utility model provides a kind of exosome culture's bioreactor, it is related to biological culture technical field, platform, the inside of the platform is provided with vibration mechanism;The vibration mechanism includes the round plate of sliding installation in the inside of platform, and the first spring is fixedly connected between the round plate and the bottom end of the inside of platform, the uniformly fixed rack of placement is placed in the bottom side of round plate, the uniformly opened placement groove of platform top side, the tank is placed in the inside of placement groove, the round table is fixed in the top side center of round plate and passes through platform, the servo motor is fixed in the top side of platform, and the rubber cam is fixed in the output end of servo motor.The utility model passes through the structure of ration mechanism, passes through the structure of vibration mechanism, passes through the structure of servo motor and rubber cam, can hit round table and drive round plate vibration, to drive tank rotation, promote inside culture solution and cell mixing evenly in tank, usually platform inside liquid can also prevent tank excessive vibration, affect the normal growth of cell.

Description

Technical Field

[0001] This invention relates to the field of biological culture technology, specifically a bioreactor for exosome culture. Background Technology

[0002] Exosomes, a term in cell biology, typically refer to vesicle structures released from the cell. They contain various bioactive molecules, such as proteins, nucleic acids, and lipids. Bioreactors are required during cell culture.

[0003] A bioreactor for stem cell exosome culture, with announcement number CN219156893U, comprises a cell culture chamber as its main body. A geared ring is fixed to the top side of the base, and a first gear is rotatably mounted inside the geared ring. A drive mechanism is provided inside the geared ring, and the rotation axis of the first gear is aligned with the height direction of the base. A second gear is rotatably mounted between the geared ring and the first gear, and the second gear meshes with both the first gear and the geared ring. The cell culture chamber is fixedly mounted on the second gear. This bioreactor, through the structure of the gears and the geared ring, can drive the cell culture chamber to rotate on its own axis while revolving around the central axis, thereby ensuring uniform mixing of the culture medium and cells inside and accelerating cell proliferation.

[0004] The bioreactor for stem cell exosome culture described above also has a problem: when the bioreactor is in use, the cell culture chamber rotates on its own axis while revolving around the sun, which makes it easy for the cell culture medium inside the cell culture chamber to seep out through the gaps in the top cover, thus affecting the culture of stem cells. Utility Model Content

[0005] The purpose of this invention is to provide a bioreactor for exosome culture, so as to solve the problems mentioned in the background art and overcome its technical defects.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows: A bioreactor for exosome culture, comprising: The platform has a vibration mechanism on its inner side, a heating mechanism on its bottom side, and a detection mechanism at its top edge. The vibration mechanism includes a circular plate slidably mounted on the inner side of the platform. A first spring is fixedly connected between the circular plate and the bottom of the inner side of the platform. Placement racks are uniformly fixed on the bottom side of the circular plate. Placement slots are uniformly opened on the top side of the platform. A sealing ring is fixed inside the placement slot. A tank is placed inside the placement slot. A frustum is fixed through the platform at the center of the top side of the circular plate. A servo motor is fixed on the top side of the platform. A rubber cam is fixed at the output end of the servo motor.

[0007] As a further embodiment of this utility model: the rubber cam is located on the top side of the frustum, the can is placed inside the placement rack, and the placement rack is in close contact with the surface of the can.

[0008] As a further embodiment of this utility model: the heating mechanism includes a water pump fixed to the bottom side of the platform, a drain pipe fixed to the input end of the water pump, and one end of the drain pipe passing through the platform and communicating with the center of its inner bottom end.

[0009] As a further improvement of this utility model: a water supply pipe is fixed to the output end of the water pump, heating wires are evenly fixed on the circumference of the drain pipe, and a water supply trough is provided at the inner edge of the platform.

[0010] As a further improvement of this utility model: water spray holes are evenly provided on the inner circumferential surface of the platform, and the water spray holes connect the inner side of the platform to the water supply tank.

[0011] As a further embodiment of this utility model: the detection mechanism includes a glass cylinder that is fixedly inserted into the top edge of the platform, the bottom end of the glass cylinder is inserted into the inside of the water tank, and a piston is slidably installed inside the glass cylinder.

[0012] As a further embodiment of this utility model: support legs are evenly fixed on the bottom side of the platform, a base is fixed at the bottom end of the support legs, and a second spring is fixedly connected between the piston and the top end of the inner side of the glass cylinder.

[0013] As a further improvement of this utility model: a through hole is opened at the center of the top of the glass tube, and a scale groove is evenly opened on one side of the circumference of the glass tube, and the glass tube is located on the top side of the water supply pipe.

[0014] Compared with the prior art, the beneficial effects of this utility model include: This invention utilizes a vibration mechanism with a servo motor and a rubber cam to strike a frustum, causing the circular plate to vibrate and thus rotating the tank. This promotes uniform mixing of the culture medium and cells inside the tank, while the liquid inside the platform prevents excessive vibration of the tank from affecting normal cell growth. Attached Figure Description

[0015] The disclosure of this utility model is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this utility model. In the drawings, the same reference numerals are used to refer to the same parts. Wherein: Figure 1 This is a schematic diagram of the structure of a bioreactor for exosome culture; Figure 2 A rear cross-sectional view of the vibration mechanism of a bioreactor for exosome culture; Figure 3 A top-view cross-sectional schematic diagram of the heating mechanism of a bioreactor for exosome culture; Figure 4 This is a side cross-sectional schematic diagram of a bioreactor detection mechanism for exosome culture; The following are the labeling elements in the diagram: 1. Platform; 2. Vibration mechanism; 21. Circular plate; 22. First spring; 23. Placement rack; 24. Placement slot; 25. Tank body; 26. Frustum; 27. Servo motor; 28. Rubber cam; 3. Support leg; 4. Base; 5. Water supply tank; 6. Spray hole; 7. Heating mechanism; 71. Water pump; 72. Water supply pipe; 73. Drain pipe; 74. Heating wire; 8. Detection mechanism; 81. Glass cylinder; 82. Piston; 83. Second spring; 84. Through hole; 85. Scale groove; 9. Sealing ring. Detailed Implementation

[0016] It is readily understood that, based on the technical solution of this utility model, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of this utility model. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative descriptions of the technical solution of this utility model and should not be considered as the entirety of this utility model or as limitations or restrictions on the technical solution of this utility model.

[0017] Example 1: Please see Figures 1-2 This is the first embodiment of the present invention. This embodiment provides a bioreactor for exosome culture, comprising: Platform 1, with a vibration mechanism 2 installed inside the platform 1, a heating mechanism 7 installed on the bottom side of the platform 1, and a detection mechanism 8 installed at the top edge of the platform 1; The vibration mechanism 2 includes a circular plate 21 slidably mounted on the inner side of the platform 1. A first spring 22 is fixedly connected between the circular plate 21 and the bottom inner side of the platform 1. Placement racks 23 are evenly fixed on the bottom side of the circular plate 21. Placement slots 24 are evenly opened on the top side of the platform 1. A sealing ring 9 is fixed inside the placement slot 24. A tank 25 is placed inside the placement slot 24. A frustum 26 is fixed through the platform 1 at the center of the top side of the circular plate 21. A servo motor 27 is fixed on the top side of the platform 1. A rubber cam 28 is fixed at the output end of the servo motor 27.

[0018] Specifically, the rubber cam 28 is located on the top side of the frustum 26, the can 25 is placed inside the placement rack 23, and the placement rack 23 is in close contact with the surface of the can 25.

[0019] Furthermore, the structure of the rubber cam 28 allows for rotation during the process, which is then transmitted to the tank 25 to ensure that the culture medium inside the tank is mixed evenly with the cells, while preventing the liquid inside the tank from seeping out or leaking.

[0020] In use, the servo motor 27 is controlled to drive the output rubber cam 28 to rotate. During the rotation of the rubber cam 28, it strikes the top side of the frustum 26, thereby causing the circular plate 21 to vibrate inside the platform 1. At this time, the tank 25 is placed in the placement rack 23 inside the placement slot 24. The circular plate 21 drives the tank 25 to rotate through the placement rack 23. At the same time, the inside of the platform 1 is filled with clean water. The sealing ring 9 can prevent the liquid inside the platform 1 from splashing and can also prevent the tank 25 from rotating excessively, which would affect cell growth. This structure can prevent the liquid inside the tank 25 from leaking and promote the uniform mixing of culture medium and cells.

[0021] In summary, through the structure of the vibration mechanism 2, and through the structure of the servo motor 27 and the rubber cam 28, the truncated cone 26 can be struck to drive the circular plate 21 to vibrate, thereby driving the tank 25 to rotate, so that the culture medium and cells inside the tank 25 are mixed evenly. Usually, the liquid inside the platform 1 can prevent the tank 25 from vibrating excessively and affecting the normal growth of cells.

[0022] Example 2: Please see Figures 3-4 This is the second embodiment of the present utility model.

[0023] Specifically, the heating mechanism 7 includes a water pump 71 fixed to the bottom side of the platform 1, a drain pipe 73 fixed to the input end of the water pump 71, one end of the drain pipe 73 passing through the platform 1 and communicating with the center of its inner bottom end, a water supply pipe 72 fixed to the output end of the water pump 71, heating wires 74 evenly fixed on the circumference of the drain pipe 73, a water supply trough 5 opened at the inner edge of the platform 1, and water spray holes 6 evenly opened on the inner circumference of the platform 1, which connect the inner side of the platform 1 with the water supply trough 5.

[0024] Furthermore, by using the heating wire 74 in conjunction with the drain pipe 73, the clean water source inside the platform 1 can be heated while flowing, so that the tank 25 is within a suitable temperature range for cell culture and growth.

[0025] Specifically, the testing mechanism 8 includes a glass cylinder 81 fixedly inserted at the top edge of the platform 1. The bottom end of the glass cylinder 81 is inserted inside the water supply tank 5. A piston 82 is slidably installed inside the glass cylinder 81. Support legs 3 are evenly fixed on the bottom side of the platform 1. A base 4 is fixed at the bottom end of the support legs 3. A second spring 83 is fixedly connected between the piston 82 and the top end of the inner side of the glass cylinder 81. A through hole 84 is opened at the center of the top end of the glass cylinder 81. Graduation grooves 85 are evenly opened on one side of the circumference of the glass cylinder 81. The glass cylinder 81 is located on the top side of the water supply pipe 72.

[0026] Furthermore, the structure of the glass tube 81 and the piston 82 allows for the detection of the temperature of the liquid output by the water pump 71, enabling timely adjustments as needed to prevent cell death during the culture process.

[0027] In use, the water pump 71 is controlled to draw liquid in through the drain pipe 73. After being heated by the heating wire 74, the liquid is transported back to the inside of the water tank 5 through the water supply pipe 72 and sprayed out through the spray hole 6. This is to adjust the temperature of the cultured cells inside the platform 1 to a suitable range. At the same time, the glass tube 81 is located on top of the water supply pipe 72. The hot water causes the air temperature inside the glass tube 81 to rise, which in turn expands and pushes the piston 82 to slide upward, compressing the second spring 83. By observing the position of the piston 82 in the scale groove 85, the problem of the liquid output of the water pump 71 can be identified, and timely adjustments can be made to ensure normal cell growth.

[0028] In summary, through the structure of the heating mechanism 7 and the detection mechanism 8, the clean water source inside the platform 1 can be circulated and heated by the water pump 71, so that the cells are in a suitable temperature range. In addition, with the cooperation of the piston 82 and the second spring 83, the temperature of the liquid output by the water pump 71 is detected to avoid cell death caused by high temperature.

[0029] The technical scope of this utility model is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this utility model, and all such modifications and variations should fall within the protection scope of this utility model.

Claims

1. A bioreactor for exosome culture, characterized in that, The platform (1) includes a vibration mechanism (2) on the inner side of the platform (1), a heating mechanism (7) on the bottom side of the platform (1), and a detection mechanism (8) on the top edge of the platform (1). The vibration mechanism (2) includes a circular plate (21) slidably mounted on the inner side of the platform (1). A first spring (22) is fixedly connected between the circular plate (21) and the bottom of the inner side of the platform (1). A placement rack (23) is evenly fixed on the bottom side of the circular plate (21). A placement groove (24) is evenly opened on the top side of the platform (1). A sealing ring (9) is fixed on the inner side of the placement groove (24). A tank (25) is placed on the inner side of the placement groove (24). A frustum (26) is fixed through the platform (1) at the center of the top side of the circular plate (21). A servo motor (27) is fixed on the top side of the platform (1). A rubber cam (28) is fixed at the output end of the servo motor (27).

2. The bioreactor for exosome culture according to claim 1, characterized in that, The rubber cam (28) is located on the top side of the frustum (26), and the can (25) is placed inside the placement rack (23), with the placement rack (23) and the surface of the can (25) closely attached.

3. The bioreactor for exosome culture according to claim 1, characterized in that, The heating mechanism (7) includes a water pump (71) fixed on the bottom side of the platform (1), and a drain pipe (73) fixed at the input end of the water pump (71). One end of the drain pipe (73) passes through the platform (1) and is connected to the center of its inner bottom end.

4. A bioreactor for exosome culture according to claim 3, characterized in that, The output end of the water pump (71) is fixed with a water delivery pipe (72), the drain pipe (73) is uniformly fixed with heating wires (74) on its circumference, and a water delivery trough (5) is provided at the inner edge of the platform (1).

5. A bioreactor for exosome culture according to claim 4, characterized in that, The platform (1) has water spray holes (6) evenly distributed on its inner circumferential surface, and the water spray holes (6) connect the inner side of the platform (1) with the water supply tank (5).

6. A bioreactor for exosome culture according to claim 1, characterized in that, The detection mechanism (8) includes a glass cylinder (81) fixedly inserted at the top edge of the platform (1), the bottom end of the glass cylinder (81) being inserted inside the water tank (5), and a piston (82) being slidably installed inside the glass cylinder (81).

7. A bioreactor for exosome culture according to claim 6, characterized in that, The platform (1) has support legs (3) evenly fixed on its bottom side, and a base (4) is fixed at the bottom end of the support legs (3). A second spring (83) is fixedly connected between the piston (82) and the top of the inner side of the glass tube (81).

8. A bioreactor for exosome culture according to claim 7, characterized in that, A through hole (84) is opened at the center of the top of the glass tube (81), and a scale groove (85) is evenly opened on one side of the circumference of the glass tube (81). The glass tube (81) is located on the top side of the water pipe (72).

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