Fermentation broth sampling valve

Abstract

The utility model relates to a fermentation liquor sampling valve relates to sampling structure technical field, including valve body, the inside horizontal fixed of valve body is equipped with valve core, and the valve core includes the conical section and the cylinder section on the big diameter section of conical section connection, and the small diameter end of conical section is close to liquid inlet, and the cylinder section extends to the liquid outlet direction, and the spiral flow guide groove is seted up on the circumference wall of cylinder section. The utility model solves the traditional fermentation liquor sampling valve in traditional technology in the long -term use process, and after the valve internal passage is blocked, the existing cleaning mode has the problem that the operation process is complicated, and external pollutants are easily introduced, and the purity and product quality of fermentation process constitute potential risk.

Description

Technical Field

[0001] This utility model relates to the field of sampling structure technology, specifically to a fermentation broth sampling valve. Background Technology

[0002] Fermentation broth refers to a liquid culture medium inoculated with microbial strains. After a period of cultivation, the microorganisms use the nutrients in the culture medium to synthesize cell bodies and secrete products. This liquid, after being metabolized by the microorganisms, is called fermentation broth. When testing is required, a sampling tube is used to collect samples.

[0003] In the existing technology, when sampling and testing fermentation broth, the fermentation broth in the container can be sampled in layers using a sampling tube. When performing layered sampling, it is crucial to be able to easily control the sampling layers and ensure precise control of the sampling tube. Therefore, it is necessary to be able to easily control the sampling volume of the sampling tube to facilitate layered sampling of the fermentation broth.

[0004] A prior art patent with publication number CN203643199U discloses a solution including a support body, a stopcock valve, an O-ring, a filter membrane tube, and a tube cap. Only the support body and the tube cap are used to fix the filter membrane tube, and the fixing method is to clamp it to both ends of the filter membrane tube. In use, this device can be installed on a fermenter, with the sampling port directly connected to the inlet of an analytical instrument. The sampling rate is controlled by the analyzer. The overall structure of the device is simple, easy to assemble and mass-produce, and has low manufacturing costs. It can easily meet the requirements of high temperature resistance and corrosion resistance, can withstand high-temperature and high-pressure sterilization, and effectively prevents bacterial contamination during use.

[0005] Existing devices, including those mentioned above, have gradually revealed shortcomings in the technology with use, mainly in the following aspects:

[0006] Traditional fermentation broth sampling valves often experience blockages in their internal channels due to the tendency of solutes in the fermentation broth to crystallize and precipitate during long-term use. This blockage not only causes uneven sampling and data distortion but may also lead to mechanical failures due to crystal accumulation. Currently, the industry's commonly used solutions mainly rely on external high-pressure flushing or mechanical crushing, but these methods have significant limitations: the operation process is cumbersome and complex, and they are prone to introducing external contaminants, posing potential risks to the purity of the fermentation process and product quality.

[0007] In conclusion, the existing technology obviously has inconveniences and defects in practical use, so it is necessary to improve it. Utility Model Content

[0008] To address the shortcomings of existing technologies, this utility model provides a fermentation broth sampling valve to solve the problem that, in the long-term use of traditional fermentation broth sampling valves, the internal channels of the valve become clogged, and the existing cleaning methods are cumbersome and complicated, and are prone to introducing external contaminants, posing a potential risk to the purity of the fermentation process and the quality of the product.

[0009] To achieve the above objectives, this utility model provides the following technical solution:

[0010] A fermentation broth sampling valve includes a valve body, inside which a valve core is horizontally fixed. The valve core includes a conical section and a cylindrical section connected to the large diameter section of the conical section. The small diameter end of the conical section is close to the inlet, and the cylindrical section extends toward the outlet. A spiral guide groove is formed on the peripheral wall of the cylindrical section.

[0011] As an optimized solution, one end of the valve body is connected to a temperature control component.

[0012] As an optimized solution, the valve body is made of medical-grade 316L stainless steel.

[0013] As an optimized solution, a high-frequency micro-vibration structure is externally fixed on the valve body.

[0014] As an optimized solution, the inlet of the valve body is equipped with a filter screen.

[0015] As an optimized solution, the valve body includes a horizontal cylindrical section and a vertical cylindrical section fixed above the horizontal cylindrical section, the vertical cylindrical section being connected to the inner cavity of the horizontal cylindrical section.

[0016] As an optimized solution, the filter screen is fixed to the upper port of the horizontal cylindrical section.

[0017] As an optimized solution, the temperature control component is located at one end of the horizontal cylinder section, and the valve core is coaxially arranged with the horizontal cylinder section.

[0018] As an optimized solution, the high-frequency micro-vibration structure is fixed to the outer wall of the horizontal cylinder section.

[0019] As an optimized solution, the cone angle of the tapered segment is 25°.

[0020] As an optimized solution, the depth of the spiral guide groove is 0.5 mm.

[0021] As an optimized solution, the width of the spiral guide groove is 2mm.

[0022] As an optimized solution, the lead angle of the spiral guide groove is 45°.

[0023] As an optimized solution, the filter screen includes a titanium alloy filter screen with a mesh size of 200 mesh.

[0024] Compared with the prior art, the beneficial effects of this utility model are:

[0025] By incorporating a valve core within the valve body, its 25° cone angle design conforms to the golden ratio of fluid dynamics. Combined with a 0.5mm deep spiral guide groove at the bottom (groove width 2mm, lead angle 45°), it can significantly enhance the fluid turbulence effect. CFD simulations have verified that this structure can stably maintain a fluid Reynolds number exceeding 5000, forming a high-intensity vortex field that effectively separates crystalline particles from the fermentation broth. Laboratory comparative data show that compared to traditional straight-through valves, this structure improves particle separation efficiency by up to 68%, while reducing pressure loss by 22%.

[0026] Using a 200-mesh titanium alloy filter (pore size 75μm) can filter impurities, prevent them from entering the valve body, and improve the cleanliness of the sample.

[0027] Heating is achieved by setting up a temperature control component, and a high-frequency micro-vibration structure is used to reduce the bonding at the crystal interface, prevent the medium from crystallizing and clogging, and achieve a self-cleaning effect. Attached Figure Description

[0028] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0029] Figure 1 This is a schematic diagram of the structure of this utility model;

[0030] Figure 2 This is a side view of the structure of this utility model.

[0031] In the diagram: 1-Horizontal cylindrical section; 2-Vertical cylindrical section; 3-Conical section; 4-Reduced diameter section; 5-Cylindrical section; 6-Spiral guide groove; 7-High-frequency micro-vibration structure; 8-Temperature control component; 9-Filter screen. Detailed Implementation

[0032] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present invention and should not be construed as limiting the scope of protection of the present invention.

[0033] like Figure 1 and Figure 2As shown, the fermentation broth sampling valve includes a valve body, and a valve core is horizontally fixed inside the valve body. The valve core includes a conical section 3 and a cylindrical section 5 connected to the large diameter section of the conical section 3. The small diameter end of the conical section 3 is close to the liquid inlet, and the cylindrical section 5 extends toward the liquid outlet. A spiral guide groove 6 is provided on the peripheral wall of the cylindrical section 5.

[0034] The end of the cylindrical section can be fixed to the valve body using a stainless steel column, or the outer wall of the cylindrical section can be fixed to the inner wall of the valve body.

[0035] A temperature control component 8 is connected to one end of the valve body.

[0036] A high-frequency micro-vibration structure 7 is fixed externally on the valve body.

[0037] The valve body is equipped with a filter screen 9 at the liquid inlet.

[0038] The valve body includes a horizontal cylindrical section 1 and a vertical cylindrical section 2 fixed above the horizontal cylindrical section 1. The vertical cylindrical section 2 is connected to the inner cavity of the horizontal cylindrical section 1.

[0039] The filter screen 9 is fixed to the upper port of the horizontal cylindrical section 1.

[0040] The temperature control component 8 is located at one end of the horizontal cylinder section 1, and the valve core is coaxially arranged with the horizontal cylinder section 1.

[0041] The other end of the horizontal cylindrical section 1 is provided with a reduced diameter section 4, and the cylindrical section 5 is located inside the reduced diameter section 4.

[0042] The high-frequency micro-vibration structure 7 is fixed on the outer wall of the horizontal cylinder section 1.

[0043] The cone angle of cone segment 3 is 25°.

[0044] The depth of the spiral guide groove 6 is 0.5mm.

[0045] The width of the spiral guide groove 6 is 2mm.

[0046] The lead angle of the spiral guide groove 6 is 45°.

[0047] The filter screen 9 includes a titanium alloy filter screen with a mesh size of 200.

[0048] The innovative design uses a 200-mesh titanium alloy filter (75μm pore size). The vertical cylinder section 2 is equipped with a self-locking buckle, which can be snapped into place with the outlet flange of the storage tank. The filter is located on the upper end of the vertical cylinder section 2, between the vertical cylinder section 2 and the outlet flange of the storage tank.

[0049] It can be quickly disassembled and assembled. The filter screen surface is plasma polished, with a surface roughness Ra≤0.2μm. The media adsorption rate is reduced to 1 / 3 of that of traditional stainless steel filter screens, making it particularly suitable for high-viscosity fermentation broth environments.

[0050] The structure of the temperature control component is common and not innovative in this solution, so it will not be elaborated upon here. For example, it integrates an embedded PTC ceramic heating element (150W power, response time <15 seconds), combined with a high-precision platinum resistance sensor (measurement error ±0.3℃) and an adaptive PID algorithm, achieving closed-loop temperature control within ±1℃. The heating zone adopts a biomimetic gradient heat dissipation groove design. By optimizing the groove depth (gradually changing from 0.8-1.5mm) and distribution density, the surface temperature difference is controlled within 3℃.

[0051] The structure of the high-frequency micro-vibration structure is common and not innovative in this solution, so it will not be elaborated upon here. For example, four sets of PZT-8 piezoelectric ceramic arrays are used in parallel to drive the system, achieving a resonant frequency of 20kHz±1% and an output force of 800N. Three-dimensional vibration (X / Y / Z axes 50 / 50 / 30μm) is transmitted through a λ / 4 titanium alloy amplitude transformer, and uniform energy distribution is achieved in conjunction with an HV2200 silicon carbide vibrating head (R5mm spherical surface). Under 60℃ thermal expansion conditions, the system reduces the crystal interface bonding energy by 42%, achieves a response time of <10ms, and an amplitude fluctuation rate of ≤3%, ultimately reaching a peeling rate of ≥99.8% without matrix damage.

[0052] The working principle of this device is as follows:

[0053] By incorporating a valve core within the valve body, its 25° cone angle design conforms to the golden ratio of fluid dynamics. Combined with a 0.5mm deep spiral guide groove at the bottom (groove width 2mm, lead angle 45°), it can significantly enhance the fluid turbulence effect. CFD simulations have verified that this structure can stably maintain a fluid Reynolds number exceeding 5000, forming a high-intensity vortex field that effectively separates crystalline particles from the fermentation broth. Laboratory comparative data show that compared to traditional straight-through valves, this structure improves particle separation efficiency by up to 68%, while reducing pressure loss by 22%.

[0054] Using a 200-mesh titanium alloy filter (pore size 75μm) can filter impurities, prevent them from entering the valve body, and improve the cleanliness of the sample.

[0055] Heating is achieved by setting up a temperature control component, and a high-frequency micro-vibration structure is used to reduce the bonding at the crystal interface, prevent the medium from crystallizing and clogging, and achieve a self-cleaning effect.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. A fermentation broth sampling valve, characterized in that: The valve includes a valve body, and a valve core is fixedly installed horizontally inside the valve body. The valve core includes a conical section (3) and a cylindrical section (5) connected to the large diameter section of the conical section (3). The small diameter end of the conical section (3) is close to the liquid inlet, and the cylindrical section (5) extends toward the liquid outlet. A spiral guide groove (6) is provided on the peripheral wall of the cylindrical section (5).

2. The fermentation broth sampling valve according to claim 1, characterized in that: The valve body is equipped with a filter screen (9) at the liquid inlet.

3. The fermentation broth sampling valve according to claim 2, characterized in that: The valve body includes a horizontal cylindrical section (1) and a vertical cylindrical section (2) fixed above the horizontal cylindrical section (1), the vertical cylindrical section (2) being connected to the inner cavity of the horizontal cylindrical section (1).

4. The fermentation broth sampling valve according to claim 3, characterized in that: The filter (9) is fixed to the upper port of the horizontal cylindrical section (1).

5. The fermentation broth sampling valve according to claim 3, characterized in that: The valve core is coaxially arranged with the horizontal cylinder section (1).

6. The fermentation broth sampling valve according to claim 1, characterized in that: The cone angle of the conical segment (3) is 25°.

7. The fermentation broth sampling valve according to claim 1, characterized in that: The spiral guide groove (6) has a groove depth of 0.5 mm.

8. The fermentation broth sampling valve according to claim 1, characterized in that: The width of the spiral guide groove (6) is 2mm.

9. The fermentation broth sampling valve according to claim 1, characterized in that: The lead angle of the spiral guide groove (6) is 45°.

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