A flow cytometer multi-channel sample splitting device

Abstract

The utility model discloses a kind of flow cytometry multi-channel sample shunt devices, the utility model relates to flow cytometry technical field.The flow cytometry multi-channel sample shunt device includes bottom plate, the top of the bottom plate is provided with shunt mechanism, for shunt to be measured, the shunt mechanism includes: flow guide component, including fixedly installed in the top of bottom plate's support frame, the top of the support frame is fixedly installed with shunt pipe, the lower portion of the shunt pipe is fixedly installed with adapter pipe, when micro electric push rod drives sliding block to slide along bracket, sliding block bottom slope and bracket groove bottom slope perfectly fit, the cooperation of both can stably clamp or loosen hose, compared with the mode of conventional manual rotary threaded rod, electric adjustment can realize the fine control of telescopic amount, and then accurately change stable adjustment liquid flow rate through hose deformation variable, control panel will continue to fine tune according to the real-time feedback of ultrasonic module, form closed-loop control.

Description

Technical Field

[0001] This utility model relates to the field of flow cytometer technology, specifically to a multi-channel sample splitting device for flow cytometers. Background Technology

[0002] A cytometer is an instrument used to analyze and measure the characteristics and functions of cells in biological samples.

[0003] The existing utility model patent with publication number CN221056293U discloses a multi-channel flow cytometer, relating to the field of flow cytometer detection technology. It includes a flow cytometer body, a flow divider mechanism at one end of the flow cytometer body, and a liquid inlet mechanism at the other end of the flow divider mechanism. This utility model, through the cooperation between the flow cytometer body, the liquid inlet mechanism, and the flow divider mechanism, without affecting the cell detection efficacy of the flow cytometer body itself, utilizes the cooperation between the liquid outlet pipe of the flow divider mechanism and the flow limiting tank and flow limiting block. This allows the flow limiting block to be disconnected from the flow limiting tank by rotating a threaded rod, and the flow rate of the liquid flowing into the flow cytometer body can be determined according to the range of rotation. Furthermore, the connecting threads in the flow divider mechanism connect with the filter in the placement tank and the liquid inlet mechanism, allowing the threaded cover to be disconnected from the connecting threads on the placement chamber by rotation, thus replacing the filter. This improves the stability and applicability of the mechanism to a certain extent.

[0004] The aforementioned cell analyzer's flow splitting mechanism controls the distance between the flow limiting block and the flow limiting tank by rotating a screw rod, thereby adjusting the flow rate. This method relies on manual operation, making precise flow rate control difficult and potentially unsuitable for experiments requiring high flow rate accuracy. Furthermore, it necessitates rotating different positions of the screw rod to control the flow rate of each outlet tube, making the operation cumbersome, especially when adjusting the flow rate of multiple channels simultaneously, increasing the operator's workload and operational difficulty. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a multi-channel sample splitting device for flow cytometers, which solves the problems of low flow rate adjustment accuracy and cumbersome operation when adjusting multiple channels.

[0006] To achieve the above objectives, this utility model provides the following technical solution: A multi-channel sample splitting device for a flow cytometer includes a base plate, and a splitting mechanism is disposed above the base plate for splitting the analyte. The splitting mechanism includes:

[0007] The flow guiding assembly includes a support frame fixedly installed above a base plate, a diversion pipe fixedly installed above the support frame, an adapter pipe fixedly installed below the diversion pipe, a flexible hose connected to the rear end of the adapter pipe, a bracket fixedly installed above the base plate, a miniature electric actuator fixedly installed above the bracket, and a slider fixedly installed at the front end of the miniature electric actuator.

[0008] The detection component, located above the base plate, is used to monitor the flow rate of the sample after diversion.

[0009] Preferably, the detection component includes a detection tube fixedly installed above the base plate, an ultrasonic module disposed above the detection tube, a partition fixedly installed inside the detection tube, connecting plates fixedly installed at the front and rear ends of the detection tube, a control board and a battery fixedly installed above the base plate, a housing disposed outside the control board and the battery, and a touch screen fixedly installed on the front side of the base plate.

[0010] Preferably, the bottom of the diversion pipe is provided with a pipe branch structure, the diversion pipe is connected to multiple hoses through an adapter pipe, and the upper part of the bracket is provided with a groove structure with a sloping bottom, and the groove structure on the upper part of the bracket matches the width of the hose.

[0011] Preferably, the slider is slidably connected to the bracket via a miniature electric actuator, and the inclined surface structure at the bottom of the slider has the same inclination angle as the inclined surface at the bottom of the bracket groove.

[0012] Preferably, the ultrasound module consists of a receiver and a generator, which are symmetrically installed above the detection tube. The partitions are installed equidistantly inside the detection tube, and a pipe structure is provided at the center of the connecting plate. The detection tube is connected to the flexible tube through the connecting plate.

[0013] Preferably, the control board and the battery are located inside the housing, the bottom end of the housing is fixedly connected to the base plate, and the front side of the base plate is provided with an inclined frame structure to support the touch screen.

[0014] Beneficial effects

[0015] This invention provides a multi-channel sample splitting device for flow cytometers. Compared with the prior art, it has the following advantages:

[0016] (1) In this multi-channel sample splitting device for flow cytometer, the ultrasound module continuously monitors the flow rate of the analyte flowing through the detection tube and transmits the flow data to the control board in real time, providing accurate data for adjustment. The control board automatically calculates the required extension and retraction of the micro electric actuator based on the deviation between the preset flow parameters and the actual monitoring data, avoiding errors in manual judgment. Secondly, when the micro electric actuator drives the slider to slide along the bracket, the bottom inclined surface of the slider perfectly fits the bottom inclined surface of the bracket groove. The two work together to stably clamp or loosen the tubing. Compared with the traditional method of manually rotating the threaded rod, electric adjustment can achieve precise control of the extension and retraction, thereby stabilizing the liquid flow rate by accurately changing the tubing deformation. At the same time, the control board will continuously fine-tune according to the real-time feedback from the ultrasound module, forming a closed-loop control to ensure that the flow rate is always stable within the preset accuracy range.

[0017] (2) This multi-channel sample splitting device for flow cytometers allows for the simultaneous connection of multiple flexible tubes via a branching structure at the bottom of the splitting tube, enabling the simultaneous establishment of multi-channel splitting paths without the need for separate splitting components for each channel. Operationally, the touchscreen provides a visual, centralized interface, eliminating the need for manual adjustment of individual channel mechanical components. Users can simply set the target flow rate for all channels or adjust parameters for a single channel via the touchscreen. Simultaneously, the control board can control the micro-electric actuators of each channel based on independent monitoring data from the ultrasound modules, enabling synchronous adjustment or independent correction of multi-channel flow rates. This significantly reduces manual operation steps and lowers the workload and operational difficulty during multi-channel adjustment. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the installation structure of the diversion pipe of this utility model;

[0020] Figure 3 This is a schematic diagram of the hose installation structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the partition installation structure of this utility model;

[0022] In the diagram: 1. Base plate; 2. Diverting mechanism; 21. Diverting assembly; 211. Support frame; 212. Diverting pipe; 213. Adapter pipe; 214. Flexible hose; 215. Bracket; 216. Miniature electric actuator; 217. Slider; 22. Detection assembly; 221. Detection tube; 222. Ultrasonic module; 223. Partition plate; 224. Connecting plate; 225. Control board; 226. Battery; 227. Outer shell; 228. Touch screen. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figure 1-4 This utility model provides a technical solution: a multi-channel sample splitting device for a flow cytometer includes a base plate 1, and a splitting mechanism 2 is disposed above the base plate 1 for splitting the analyte. The splitting mechanism 2 includes:

[0025] The flow guiding assembly 21 includes a support frame 211 fixedly installed above the base plate 1. A diversion pipe 212 is fixedly installed above the support frame 211, and an adapter pipe 213 is fixedly installed below the diversion pipe 212. A flexible hose 214 is connected to the rear end of the adapter pipe 213. A bracket 215 is fixedly installed above the base plate 1, and a miniature electric actuator 216 is fixedly installed above the bracket 215. A slider 217 is fixedly installed at the front end of the miniature electric actuator 216. The bottom of the diversion pipe 212 is provided with a pipe branch structure. The diversion pipe 212 is connected to multiple flexible hoses 214 through the adapter pipe 213. The top of the bracket 215 is provided with a groove structure with a sloping bottom, and the groove structure on the top of the bracket 215 matches the width of the flexible hose 214. The slider 217 is slidably connected to the bracket 215 through the miniature electric actuator 216. The sloping bottom structure of the slider 217 has the same inclination angle as the sloping bottom of the groove of the bracket 215.

[0026] Specifically, the shunt tube 212 allows the test solution to enter the lower transfer tube 213 through its branch structure. After passing through the transfer tube 213, the test solution enters the rear hose 214 and then the detection assembly 22. The micro electric actuator 216 drives the slider 217 to move, so that the bottom of the slider 217 engages with the inclined structure inside the groove of the bracket 215 to clamp the hose 214. The degree of deformation of the hose 214 is controlled by adjusting the extension and retraction of the micro electric actuator 216, thereby limiting the flow rate.

[0027] The detection component 22, located above the base plate 1, is used to monitor the flow rate of the sample after diversion. The detection component 22 includes a detection tube 221 fixedly installed above the base plate 1, an ultrasonic module 222 positioned above the detection tube 221, a partition 223 fixedly installed inside the detection tube 221, and connecting plates 224 fixedly installed at both ends of the detection tube 221. A control board 225 and a battery 226 are fixedly installed above the base plate 1, and a housing 227 is provided outside the control board 225 and the battery 226. The front of the base plate 1... A touch screen 228 is fixedly installed on the side. The ultrasound module 222 is divided into a receiver and a generator, which are symmetrically installed above the detection tube 221. The partition 223 is installed at equal intervals inside the detection tube 221. A pipe structure is set in the center of the connecting plate 224. The detection tube 221 is connected to the hose 214 through the connecting plate 224. The control board 225 and the battery 226 are located inside the housing 227. The bottom end of the housing 227 is fixedly connected to the base plate 1. An inclined frame structure is set on the front side of the base plate 1 to support the touch screen 228.

[0028] Specifically, the partition 223 restricts the test object to pass through the detection tube 221 relatively evenly, the ultrasonic module 222 can monitor the flow rate of the liquid, the control board 225 can fine-tune the extension and retraction of the micro electric actuator 216 based on the monitoring results of the ultrasonic module 222, the battery 226 can power the ultrasonic module 222 and the micro electric actuator 216, and the touch screen 228 can easily adjust the flow rate of each branch.

[0029] Specifically, the model number of the miniature electric actuator 216 is N-216-NEXLINE, and the model number of the ultrasonic module 222 is Microsonic-22221. In addition, all contents not described in detail in this specification are prior art known to those skilled in the art.

[0030] During operation, the test object first enters the diversion pipe 212 fixed above the support frame 211. After initial diversion through the pipe branch structure at the bottom of the diversion pipe 212, it flows into the hose 214 connected to the rear end through the adapter pipe 213 fixed below the diversion pipe 212. The hose 214 fits perfectly into the groove of the bracket 215 fixed above the base plate 1. Subsequently, the test object in the hose 214 enters the detection pipe 221 through the connecting plates 224 fixed at both ends of the detection pipe 221. The baffles 223 installed at equal intervals inside the detection pipe 221 ensure that the test object flows evenly through the detection pipe 221. At the same time, the ultrasonic modules 222 symmetrically installed above the detection pipe 221 monitor the flow rate of the test object in real time and transmit the monitoring data. The data is fed to a control board 225 fixed above the base plate 1. Based on the monitoring data from the ultrasound module 222, the control board 225 automatically controls the extension and retraction of the miniature electric actuator 216 fixed above the bracket 215, which drives the slider 217 fixed at the front end of the miniature electric actuator 216 to slide along the bracket 215. The slider 217 clamps or loosens the hose 214 by cooperating with the inclined surface of the bracket 215, thereby precisely controlling the flow rate by adjusting the deformation of the hose 214. The battery 226 supplies power to the ultrasound module 222, the miniature electric actuator 216, the control board 225 and other components. The touch screen 228 fixed on the front side of the base plate 1 can be used to manually set or adjust the target flow parameters of each channel, ultimately achieving precise diversion and real-time monitoring of multi-channel samples.

[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multi-channel sample splitter for a flow cytometer, characterized in that: The test sample includes a base plate (1), and a flow-diverting mechanism (2) is provided above the base plate (1) for diverting the test sample. The flow-diverting mechanism (2) includes: The flow guiding assembly (21) includes a support frame (211) fixedly installed above the base plate (1), a diversion pipe (212) fixedly installed above the support frame (211), an adapter pipe (213) fixedly installed below the diversion pipe (212), a hose (214) connected to the rear end of the adapter pipe (213), a bracket (215) fixedly installed above the base plate (1), a miniature electric actuator (216) fixedly installed above the bracket (215), and a slider (217) fixedly installed at the front end of the miniature electric actuator (216). The detection component (22) is set above the base plate (1) to monitor the flow rate of the object to be measured after diversion.

2. The multi-channel sample splitter for a flow cytometer according to claim 1, characterized in that: The detection component (22) includes a detection tube (221) fixedly installed above the base plate (1), an ultrasonic module (222) is provided above the detection tube (221), a partition (223) is fixedly installed inside the detection tube (221), a connecting plate (224) is fixedly installed at the front and rear ends of the detection tube (221), a control board (225) and a battery (226) are fixedly installed above the base plate (1), a shell (227) is provided on the outside of the control board (225) and the battery (226), and a touch screen (228) is fixedly installed on the front side of the base plate (1).

3. The multi-channel sample splitter for a flow cytometer according to claim 1, characterized in that: The bottom of the diversion pipe (212) is provided with a pipe branch structure. The diversion pipe (212) is connected to multiple hoses (214) through the adapter pipe (213). The bracket (215) is provided with a groove structure with a sloping bottom on the top, and the groove structure on the top of the bracket (215) matches the width of the hose (214).

4. The multi-channel sample splitter for a flow cytometer according to claim 1, characterized in that: The slider (217) is connected to the bracket (215) by a miniature electric actuator (216), and the bottom slope structure of the slider (217) is at the same angle as the slope at the bottom of the groove of the bracket (215).

5. A multi-channel sample splitter for a flow cytometer according to claim 2, characterized in that: The ultrasound module (222) is divided into a receiver and a generator, which are symmetrically installed above the detection tube (221). The partition (223) is installed at equal intervals inside the detection tube (221). The center of the connecting plate (224) is provided with a pipe structure. The detection tube (221) is connected to the hose (214) through the connecting plate (224).

6. A multi-channel sample splitter for a flow cytometer according to claim 2, characterized in that: The control board (225) and the battery (226) are located inside the housing (227). The bottom end of the housing (227) is fixedly connected to the base plate (1). The front side of the base plate (1) is provided with an inclined frame structure to support the touch screen (228).

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