Biocontrol agent low-temperature storage and recovery integrated device

Abstract

The utility model discloses a biocontrol agent low temperature preservation and recovery integration device belongs to biocontrol agent preservation and recovery technical field, include: constant temperature incubator and liquid storage tank, install frame is set up in constant temperature incubator, install frame is "U" shape setting, install frame is equipped with a plurality of compatible material containing frame in, constant temperature incubator inner wall one side is equipped with the locating frame, positioning shaft is set up in the locating frame, locating frame one side is equipped with a plurality of fixed plate, and a plurality of fixed plate are located on a plurality of material containing frame upper end respectively, the utility model discloses, through the rotatable spray tray of being configured at a plurality of fixed plate bottom end, and cooperate with the material containing frame that has up and down vibration function, can make the protection agent or rehydration agent and the pine wood nematode disease biocontrol agent realizes full and even mixing, effectively guarantees the effect of pine wood nematode disease biocontrol agent in low temperature preservation and recovery process, and further significantly improves the practicality of biocontrol agent low temperature preservation and recovery integration device.

Description

Technical Field

[0001] This utility model belongs to the field of biocontrol agent preservation and resuscitation technology, specifically relating to an integrated device for low-temperature preservation and resuscitation of biocontrol agents. Background Technology

[0002] The integrated low-temperature preservation and resuscitation device for biocontrol agents against pine wilt disease is a technological device developed for the prevention and control of pine wilt disease. Its core definition is a device that achieves long-term stable preservation and rapid resuscitation of biocontrol agents through the synergistic effect of a low-temperature environment and an intelligent resuscitation system. This integrated device extends the shelf life of biocontrol agents, solves the problems of easy inactivation of traditional liquid formulations and low resuscitation rates of powder formulations, and improves control efficiency. This device is widely used in the management of pine wilt disease epidemic areas, such as infested wood treatment and forest biological control. It can be combined with the release technology of natural enemy insects such as the flower beetle and the tube-shaped parasitic wasp to form a green control system of "immune induction + highly efficient killing," providing key technical support for the regional sustainable control of pine wilt disease.

[0003] Currently, in the application of integrated cryopreservation and resuscitation devices for biocontrol agents, protective agents are generally used to enhance the safety of bacterial cell preservation, and rehydration agents are used to achieve the resuscitation of biocontrol agents against pine wilt disease. However, in actual operation, protective agents or rehydration agents are usually added directly into the bacterial cells. This treatment method is prone to causing excessive accumulation of the two reagents in local areas of the bacterial cells, resulting in uneven mixing. This weakens the cryopreservation effect and resuscitation efficiency, making the practicality of integrated cryopreservation and resuscitation devices for biocontrol agents somewhat lacking. Utility Model Content

[0004] This invention proposes an integrated device for the cryogenic preservation and resuscitation of biocontrol agents to address the problem of insufficient uniformity in the mixing of biocontrol agents with protective agents or rehydration agents in existing technologies.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an integrated device for the cryopreservation and resuscitation of biocontrol agents, comprising: Incubators and liquid storage tanks; An installation frame is set inside a constant temperature incubator. The installation frame is U-shaped and contains multiple matching material holding frames. A positioning frame is provided on one side of the inner wall of the constant temperature incubator. A positioning shaft is set inside a positioning frame. A plurality of fixing plates are provided on one side of the positioning frame, and the plurality of fixing plates are respectively located at the upper ends of a plurality of material holding frames. Multiple spray discs are respectively disposed at the bottom of multiple fixed plates. Each of the multiple fixed plates is provided with an installation shaft at its upper end. The multiple installation shafts pass through the multiple fixed plates and are rotatably connected to the fixed plates.

[0006] To ensure uniform mixing of the biocontrol agent and protective agent or rehydration agent for pine wilt disease, multiple spray nozzles are provided at the bottom of each of the multiple spray trays, and multiple mounting shafts are provided with mounting holes that connect to the spray trays. A water outlet pipe is provided on one side of the liquid storage tank, and multiple infusion pipes that are adapted to the mounting holes are provided on one side of the water outlet pipe. One end of each of the multiple infusion pipes is located in one of the multiple mounting holes and is rotatably connected to the inner wall of the mounting hole.

[0007] In a preferred embodiment, a pump body is provided on one side of the constant temperature incubator, and the pump body is connected to the water outlet pipe.

[0008] In a preferred embodiment, pulleys are provided on the outer sides of the positioning shaft and the plurality of mounting shafts, and two pulleys located on the same side are provided with the same transmission belt.

[0009] In a preferred embodiment, the bottom of the constant temperature incubator is provided with a drive motor, and the bottom of the positioning shaft passes through the positioning frame and the constant temperature incubator, and is located on the output end of the drive motor.

[0010] In a preferred embodiment, the constant temperature incubator has a drive shaft at the bottom inside, a cam on the outside of the drive shaft, the upper end of the cam abutting against the bottom of the mounting frame, one end of the drive shaft extending into the positioning frame and having a first bevel gear, and a second bevel gear meshing with the first bevel gear on the outside of the positioning shaft.

[0011] In a preferred embodiment, the constant temperature incubator is provided with multiple guide rods, which pass through both sides of the mounting frame and are slidably connected to the mounting frame. Each guide rod is fitted with a limiting spring at its upper end, and the two ends of the limiting spring are respectively located on the upper end of the mounting frame and the inner wall of the constant temperature incubator.

[0012] To facilitate the handling or placement of the biocontrol bacteria for pine wilt disease, the multiple material holding frames are further provided with limiting plates on both sides, and the inner walls of the mounting frame are provided with limiting grooves that are adapted to the limiting plates. The multiple limiting plates are respectively located in the multiple limiting grooves and are slidably connected to the side walls of the limiting grooves.

[0013] Compared with the prior art, the beneficial effects of this utility model are: This invention, by setting a rotatable spray plate at the bottom of multiple fixed plates and cooperating with a material holding frame with up and down vibration function, enables the protective agent or rehydration agent to be fully and evenly mixed with the biocontrol bacteria for pine wilt disease, effectively ensuring the effect of the biocontrol bacteria for pine wilt disease in the process of low temperature preservation and recovery, and thus significantly improving the practical performance of the integrated device for low temperature preservation and recovery of biocontrol agents. This invention, through multiple limiting grooves set on both sides of the inner wall of the mounting frame, and in cooperation with the limiting plates on both sides of the material holding frame, realizes the flexible movement of the material holding frame. Operators can easily pull the material holding frame to the outside of the device, thereby conveniently completing the handling and placement of pine wilt disease biocontrol bacteria, significantly improving the operational convenience of the integrated device for low-temperature preservation and resuscitation of biocontrol agents. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the main appearance of the structure of this utility model; Figure 2 This is a side sectional view of the structure of this utility model; Figure 3 This is a schematic front cross-sectional view of the structure of this utility model; Figure 4 for Figure 2 A magnified schematic diagram of the structure at point A in the middle.

[0015] In the diagram: 1. Constant temperature incubator; 2. Liquid storage tank; 3. Mounting frame; 4. Material holding frame; 5. Positioning frame; 6. Positioning shaft; 7. Fixing plate; 8. Spray plate; 9. Mounting shaft; 10. Spray nozzle; 11. Water outlet pipe; 12. Infusion pipe; 13. Pump body; 14. Transmission belt; 15. Drive motor; 16. Transmission shaft; 17. Cam; 18. First bevel gear; 19. Second bevel gear; 20. Guide rod; 21. Limiting spring; 22. Limiting plate. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Example

[0017] Please see Figure 1-4 This utility model provides an integrated device for the cryopreservation and resuscitation of biocontrol agents, comprising: Incubator 1 and liquid storage tank 2; The mounting frame 3 is set inside the constant temperature incubator 1. The mounting frame 3 is U-shaped and has multiple matching material holding frames 4 inside. A positioning frame 5 is provided on one side of the inner wall of the constant temperature incubator 1. The positioning shaft 6 is set inside the positioning frame 5. Multiple fixing plates 7 are provided on one side of the positioning frame 5. The multiple fixing plates 7 are respectively located on the upper end of multiple material holding frames 4. Multiple spray discs 8 are respectively set at the bottom of multiple fixed plates 7. Each of the multiple fixed plates 7 is provided with a mounting shaft 9 at the top. The multiple mounting shafts 9 pass through the multiple fixed plates 7 and are rotatably connected to the fixed plates 7.

[0018] Specifically, such as Figure 2 As shown, multiple spray nozzles 10 are provided at the bottom of multiple spray plates 8, and multiple mounting shafts 9 are provided through mounting holes that connect to the spray plates 8. A water outlet pipe 11 is provided on one side of the liquid storage tank 2, and multiple infusion pipes 12 that are adapted to the mounting holes are provided on one side of the water outlet pipe 11. One end of each infusion pipe 12 is located in a multiple mounting hole and is rotatably connected to the inner wall of the mounting hole.

[0019] Through its design, the protective agent or rehydration agent in the storage tank 2 can enter the mounting holes in multiple mounting shafts 9 through the water outlet pipe 11 and multiple infusion pipes 12, thereby entering multiple spray plates 8 respectively, and being discharged through multiple spray nozzles 10. The infusion pipes 12, which are rotatably connected to the inner wall of the mounting holes, can effectively allow the protective agent or rehydration agent to enter the spray plates 8 without affecting the rotation of the spray plates 8 and the mounting shafts 9. The rotatable spray plates 8 and multiple spray nozzles 10 allow the protective agent or rehydration agent to fall evenly into multiple material collection frames 4, ensuring the uniformity of the mixing of the pine wilt disease biocontrol bacteria and the protective agent or rehydration agent.

[0020] Specifically, such as Figure 2 As shown, a pump body 13 is provided on one side of the constant temperature incubator 1. The pump body 13 is existing technology and will not be described in detail here. The pump body 13 is connected to the water outlet pipe 11. The pump body 13 can deliver the protective agent or rehydration agent to multiple infusion pipes 12 through the water outlet pipe 11, and deliver the protective agent or rehydration agent to multiple spray plates 8 through the multiple infusion pipes 12.

[0021] Specifically, such as Figure 2 As shown, pulleys are provided on the outer sides of the positioning shaft 6 and multiple mounting shafts 9. Two pulleys on the same side are provided with the same transmission belt 14. The positioning shaft 6 can drive multiple mounting shafts 9 and multiple spray discs 8 to rotate simultaneously through multiple transmission belts 14, so that the protective agent or rehydration agent can be evenly entered into the material holding frame 4 and mixed evenly with the pine wilt disease biocontrol bacteria, thereby improving the preservation and recovery effect of the pine wilt disease biocontrol bacteria.

[0022] Specifically, such as Figure 2 As shown, the bottom of the constant temperature incubator 1 is equipped with a drive motor 15. The drive motor 15 is existing technology and will not be described in detail here. The bottom of the positioning shaft 6 passes through the positioning frame 5 and the constant temperature incubator 1 and is set on the output end of the drive motor 15.

[0023] Specifically, such as Figure 2 and Figure 3As shown, the constant temperature incubator 1 has a drive shaft 16 at the bottom inside, and a cam 17 on the outside of the drive shaft 16. The upper end of the cam 17 abuts against the bottom of the mounting frame 3. One end of the drive shaft 16 extends into the positioning frame 5 and is equipped with a first bevel gear 18. The positioning shaft 6 has a second bevel gear 19 on the outside that meshes with the first bevel gear 18. When the positioning shaft 6 rotates, it will drive the drive shaft 16 to rotate through the second bevel gear 19 and the first bevel gear 18. The drive shaft 16 will drive the cam 17 to rotate, which can intermittently push the mounting frame 3 and multiple material holding frames 4 to move up and down. This can adjust the position of the pine wilt disease biocontrol bacteria in the material holding frames 4, further improving the uniformity of the mixing of the pine wilt disease biocontrol bacteria with the protectant or rehydration agent.

[0024] Specifically, such as Figure 3 As shown, the constant temperature incubator 1 is equipped with multiple guide rods 20, which pass through both sides of the mounting frame 3 and are slidably connected to the mounting frame 3. The multiple guide rods 20 can limit the vertical movement of the mounting frame 3 and the multiple material holding frames 4 to prevent their positions from shifting. Each of the multiple guide rods 20 is fitted with a limiting spring 21 at its upper end. The two ends of the limiting spring 21 are respectively set on the upper end of the mounting frame 3 and the inner wall of the constant temperature incubator 1. The multiple limiting springs 21 can drive the mounting frame 3 and the multiple material holding frames 4 to return to their original positions when the mounting frame 3 is separated from the cam 17, so that they can achieve the effect of reciprocating vertical movement. Example

[0025] Please see Figure 1-4 This utility model provides an integrated device for the cryopreservation and resuscitation of biocontrol agents, comprising: Incubator 1 and liquid storage tank 2; The mounting frame 3 is set inside the constant temperature incubator 1. The mounting frame 3 is U-shaped and has multiple matching material holding frames 4 inside. A positioning frame 5 is provided on one side of the inner wall of the constant temperature incubator 1. The positioning shaft 6 is set inside the positioning frame 5. Multiple fixing plates 7 are provided on one side of the positioning frame 5. The multiple fixing plates 7 are respectively located on the upper end of multiple material holding frames 4. Multiple spray discs 8 are respectively set at the bottom of multiple fixed plates 7. Each of the multiple fixed plates 7 is provided with a mounting shaft 9 at the top. The multiple mounting shafts 9 pass through the multiple fixed plates 7 and are rotatably connected to the fixed plates 7.

[0026] Specifically, such as Figure 3 As shown, each of the multiple material holding frames 4 has a limiting plate 22 on both sides, and the inner wall of the mounting frame 3 has a limiting groove adapted to the limiting plate 22 on both sides. The multiple limiting plates 22 are located in the multiple limiting grooves and are slidably connected to the side wall of the limiting groove.

[0027] Through its design, the multiple limiting grooves in the mounting frame 3, together with the limiting plates 22 on both sides of the multiple material holding frames 4, allow the multiple material holding frames 4 to move outward, thereby facilitating the removal or placement of biocontrol bacteria for pine wilt disease, making the integrated device for low-temperature preservation and resuscitation of biocontrol agents more convenient to use.

[0028] See Figure 1-4 When using the integrated device for cryopreservation and resuscitation of biocontrol agents, the biocontrol agents for pine wilt disease are first placed in multiple holding frames 4, and then the multiple holding frames 4 are pushed into the installation frame 3. During cryopreservation of the biocontrol agents for pine wilt disease, the pump body 13 and drive motor 15 need to be started. The pump body 13 draws the protective agent from the storage tank 2 into multiple delivery pipes 12 through the outlet pipe 11. The protective agent is then delivered to multiple spray plates 8 through the multiple delivery pipes 12. When the protective agent enters the multiple spray plates 8, it is sprayed out through multiple spray nozzles 10, ensuring that the protective agent is evenly distributed into the holding frames 4. When the drive motor 15 starts, its output end drives the positioning shaft 6 to rotate. The positioning shaft 6 drives multiple installation shafts 9 through multiple transmission belts 14, thereby driving multiple spray nozzles 9 to rotate. The rotation of the drip tray 8 improves the uniformity of the protective agent entering the holding frame 4. When the positioning shaft 6 rotates, it also drives the transmission shaft 16 to rotate through the second bevel gear 19 and the first bevel gear 18. The transmission shaft 16 drives the cam 17 to rotate. With the help of multiple limit springs 21, the mounting frame 3 and multiple holding frames 4 can be moved up and down to adjust the position of the pine wilt disease biocontrol bacteria in the holding frame 4, so that the protective agent can be evenly mixed with the pine wilt disease biocontrol bacteria. When it is necessary to revive the pine wilt disease biocontrol bacteria, the protective agent in the storage tank 2 needs to be replaced with a rehydration agent so that the rehydration agent can be evenly mixed with the pine wilt disease biocontrol bacteria, thereby improving the revival effect of the pine wilt disease biocontrol bacteria. This makes the integrated device for low-temperature preservation and revival of biocontrol agents more practical.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An integrated device for cryopreservation and resuscitation of biocontrol agents, characterized in that, include: Incubator (1) and liquid storage tank (2); The mounting frame (3) is set inside the constant temperature incubator (1). The mounting frame (3) is U-shaped. Multiple matching material holding frames (4) are provided inside the mounting frame (3). A positioning frame (5) is provided on one side of the inner wall of the constant temperature incubator (1). The positioning shaft (6) is set inside the positioning frame (5). The positioning frame (5) has multiple fixing plates (7) on one side, and the multiple fixing plates (7) are respectively located at the upper end of multiple material holding frames (4). Multiple spray discs (8) are respectively set at the bottom of multiple fixed plates (7). Each of the multiple fixed plates (7) is provided with an installation shaft (9) at its upper end. The multiple installation shafts (9) pass through the multiple fixed plates (7) and are rotatably connected to the fixed plates (7).

2. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: Each of the multiple spray plates (8) has multiple spray nozzles (10) at its bottom end. Each of the multiple mounting shafts (9) has a mounting hole through which the spray plates (8) are connected. The liquid storage tank (2) has a water outlet pipe (11) on one side. The water outlet pipe (11) has multiple infusion pipes (12) that are adapted to the mounting holes on one side. One end of each of the multiple infusion pipes (12) is located in a multiple mounting hole and is rotatably connected to the inner wall of the mounting hole.

3. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 2, characterized in that: The constant temperature incubator (1) is equipped with a pump body (13) on one side, and the pump body (13) is connected to the water outlet pipe (11).

4. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: The positioning shaft (6) and the multiple mounting shafts (9) are all provided with pulleys on their outer sides, and the two pulleys on the same side are provided with the same transmission belt (14).

5. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: The bottom of the constant temperature incubator (1) is equipped with a drive motor (15), and the bottom of the positioning shaft (6) passes through the positioning frame (5) and the constant temperature incubator (1), and is set on the output end of the drive motor (15).

6. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: The constant temperature incubator (1) has a drive shaft (16) at the bottom inside. The drive shaft (16) has a cam (17) on the outside. The upper end of the cam (17) abuts against the bottom of the mounting frame (3). One end of the drive shaft (16) extends into the positioning frame (5) and is provided with a first bevel gear (18). The positioning shaft (6) has a second bevel gear (19) on the outside that meshes with the first bevel gear (18).

7. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: The constant temperature incubator (1) is provided with multiple guide rods (20), which pass through both sides of the mounting frame (3) and are slidably connected to the mounting frame (3). Each of the multiple guide rods (20) is fitted with a limiting spring (21) at its upper end. The two ends of the limiting spring (21) are respectively set on the upper end of the mounting frame (3) and the inner wall of the constant temperature incubator (1).

8. The integrated device for cryopreservation and resuscitation of biocontrol agents according to claim 1, characterized in that: Each of the multiple material holding frames (4) is provided with a limiting plate (22) on both sides. Each of the inner walls of the mounting frame (3) is provided with a limiting groove that is compatible with the limiting plate (22). The multiple limiting plates (22) are located in the multiple limiting grooves and are slidably connected to the side wall of the limiting groove.

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