FY-07 deep fermentation system culture tank

Abstract

The utility model discloses a kind of FY-07 deep fermentation system culture tanks, mainly related to FY-07 deep fermentation technical innovation field. Including tank body, the upper portion of the tank body is equipped with the annular boss fixedly installed relative to it, the annular boss is supported with hanger, the hanger includes multiple groups of fender, the fender is equipped with the socket that passes through up and down, the socket is movably inserted with hanger plate, the hanger plate top side is equipped with the baffle that cannot pass through socket, the scraper bottom side is located in the middle or lower portion of tank body. The utility model has the beneficial effect that: cellulose can be increased by hanger plate and assisted to exhaust upwards, improve cellulose production.

Description

Technical Field

[0001] This utility model relates to the field of FY-07 deep fermentation technology innovation, specifically an FY-07 deep fermentation system culture tank. Background Technology

[0002] In the commercialization of bacterial cellulose fermentation, Kosakonia oryzednophytica FY-07 (formerly Enterobacter sp. FY-07, FY-07) is a promising candidate for industrial production. It can rapidly produce large quantities of bacterial cellulose under aerobic, limited-aerobic, and anaerobic conditions, thus enabling deep fermentation. Compared with Staphylococcus aureus, it significantly reduces fermentation time while greatly increasing yield.

[0003] However, FY07 produces a large amount of gas during submerged fermentation, including carbon dioxide, hydrogen, and residual air / oxygen. The production of these gases is substantial, with carbon dioxide being the dominant component. Excessive gas bubbles can negatively impact the fermentation process and its effectiveness. Furthermore, excessively high CO2 partial pressure may inhibit cell growth and product synthesis. Therefore, effectively managing and controlling gas production has become a challenge in controlling fermentation efficiency and cellulose production.

[0004] During the submerged fermentation of FY-07, the product system is highly variable and complex, exhibiting a heterogeneous and dynamically changing three-phase mixture of solid, liquid, and gas. The liquid phase is the culture medium, while the solid phase consists of gradually produced cellulose. In submerged fermentation, the cellulose exists as a gel in static culture and as a flocculent suspension in dynamic culture (with stirring). Cellulose does not form a complete membrane but exists as cellulose microfibrils. These microfibrils intertwine to form flocculent aggregates, particles, or spheres of varying sizes. The gas phase consists of a large amount of carbon dioxide gas produced by bacterial metabolism, dispersed in the system as bubbles. Even in dynamic fermentation, it is not easy to expel this gas in a timely manner from the viscous reaction system. High concentrations of carbon dioxide reduce cellulose yield, making the degassing problem a significant challenge.

[0005] In addition, FY07 possesses a unique "adhesive production" characteristic, making its bacterial cellulose production more favorable in environments with attached organisms. Therefore, in some technical discussions, Yoshino et al. designed a special culture system using static culture of *Acetobacter pasteurella* AP 1SK to ferment cellulose. This system involved placing a 100 μm thick layer of oxygen-permeable silica gel at the bottom of a cylindrical container, allowing *Acetobacter pasteurella* to generate a cellulose film on the oxygen-permeable rough silica gel membrane and the liquid surface. This doubled the yield of bacterial cellulose. Summary of the Invention

[0006] The purpose of this invention is to provide an FY-07 deep fermentation system culture tank, which can increase cellulose production and assist in upward exhaust by using hanging plates.

[0007] To achieve the above objectives, this utility model employs the following technical solution: An FY-07 deep fermentation system culture tank includes a tank body. The upper part of the tank body is provided with an annular boss that is fixedly installed thereon. A hanging bracket is supported on the annular boss. The hanging bracket includes multiple sets of baffles. The baffles are provided with through-holes. A hanging plate is movably inserted into the through-hole. The top side of the hanging plate is provided with a baffle that cannot pass through the through-hole. The bottom side of the scraper is located in the middle or lower part of the tank body.

[0008] The bracket includes a ring-shaped component, the outer diameter of which is larger than the inner diameter of the ring-shaped boss, and the stop bar is fixed to the ring-shaped component.

[0009] The annular boss has a mounting ring fixedly connected to it on its outer periphery. The mounting ring is cylindrical and its outer diameter is adapted to the inner diameter of the tank. Multiple bolts pass through the side wall of the mounting ring and fix the mounting ring to the tank through the bolts. The bolt heads protrude and are exposed inside the mounting ring. The annular boss has equidistant relief grooves on its circumference, and the relief grooves correspond to the spacing and number of bolts.

[0010] The inner ring of the annular boss has two L-shaped locking blocks symmetrically arranged along its circumference. The locking blocks extend upward and the maximum distance between the two locking blocks is greater than the inner diameter of the annular part. The inner side of the annular part has two or four relief grooves circumferentially arranged. The relief grooves can allow the annular part to fall onto the annular boss through the locking blocks.

[0011] The baffles are arranged at equal intervals on the annular component.

[0012] The baffles are arranged in a circular array on the ring-shaped component.

[0013] The annular component has a coaxial inner ring component at its center, and the inner ends of the baffles are all fixed to the inner ring component.

[0014] The mounting plate is provided with an air guide groove along its length, and the length of the air guide groove extends through the length of the mounting plate.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows: The hanging plates extend deep into the reaction system, providing a large surface area within a limited volume, offering attachment points to increase bacterial cellulose production. The vertically parallel plate structure minimizes flow obstruction. Liquid and bacteria can flow easily between the plates, ensuring nutrient supply and metabolite diffusion. Simultaneously, the vertically suspended plates facilitate the attachment and collection of microbubbles, gradually promoting bubble coalescence and growth, and facilitating their exit from the system. This provides a relatively smooth, upward-moving "highway" for the bubbles, allowing them to ascend more smoothly and without detours, reducing the time bubbles spend circulating and lingering within the tank. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the internal structure of Embodiment 1 of this utility model.

[0017] Figure 2 This is a schematic diagram of the engagement of the annular boss and the hanger in Embodiment 1.

[0018] Figure 3 This is a schematic diagram showing the disassembled components of the annular boss and hanger in Embodiment 1.

[0019] Figure 4 This is a schematic diagram of the fit between the bracket part and the annular boss in Embodiment 2.

[0020] Figure 5 This is a schematic diagram showing the disassembled components of the bracket and the annular boss in Embodiment 2.

[0021] The labels shown in the attached diagram: 1. Tank body; 2. Mounting ring; 3. Bolts; 4. Annular boss; 5. Reinforcing rib; 6. Annular part; 7. Leaving groove; 8. Locking block; 9. Stop bar; 10. Socket; 11. Hanging plate; 12. Baffle; 13. Air guide groove; 14. Inner ring part. Detailed Implementation

[0022] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the present invention, and these equivalent forms also fall within the scope defined in this application.

[0023] Example 1:

[0024] This example addresses the characteristics and challenges of the FY-07 fermentation system by designing a reaction vessel, including a tank 1. The tank 1 can be any fermenter commonly used for FY-07 bacterial fermentation. The components and pathways of the fermenter itself are not modified, and any fermenter of any structure, model, or capacity can be used as the tank 1.

[0025] The top of the tank body 1 is provided with a tank cover, and the top of the tank body 1 is provided with a tank opening that seals with the tank cover. The tank cover and the tank opening of the tank body 1 are connected in a sealable manner and can be opened and closed by mechanical power, such as a hydraulic pull rod or other opening mechanism, or by manual or hoisting.

[0026] A mounting ring 2 is fixed 10-100cm below the can opening. The mounting ring 2 is a metal ring 6 with an outer diameter adapted to the inner diameter of the can body 1. Multiple bolts 3 penetrate the side wall of the mounting ring 2, and the mounting ring 2 is fixed inside the can body 1 by the bolts 3. The bolt heads of the bolts 3 protrude and are exposed inside the mounting ring 2.

[0027] An annular boss 4 is fixed near the lower part of the inner ring of the mounting ring 2. A triangular reinforcing rib 5 is provided between the bottom of the annular boss 4 and the inner wall of the mounting ring 2 to improve the support strength of the annular boss 4. A bracket is placed on the annular boss 4. The bracket includes an annular component 6. The outer diameter of the annular component 6 is larger than the inner diameter of the annular boss 4, so that it can be placed on the annular boss 4. The annular component 6 has equidistant relief grooves 7 on its circumference. The relief grooves 7 correspond to the spacing and number of bolt components 3 and can cooperate with the screw head. By aligning the relief grooves 7 with the upper and lower positions of the bolt components 3, the annular component 6 can be placed downward on the annular boss 4. Then, by rotating it at a certain angle, the screw head of the bolt component 3 can block and lock the annular component 6, preventing the annular component 6 from being affected by the buoyancy of the fermentation system and fluid disturbance during fermentation, and maintaining the stability of its placement relative to the annular boss 4.

[0028] Multiple sets of baffles 9 are fixedly welded to the upper surface of the annular component 6. Each set of baffles 9 consists of two baffles 9. The two baffles 9 in the same set form an elongated insertion port 10. A hanging plate 11 is movably inserted into the insertion port 10. A baffle 12 is fixed to the top side of the hanging plate 11. The baffle 12 and the hanging plate 11 form a T-shaped structure. The bottom side of the hanging plate 11 extends into the middle and lower part of the reaction system. The hanging plate 11 itself has the effect of gathering and guiding bubbles. Furthermore, a gas guiding groove 13 is provided on the hanging plate 11 along its length direction. The length of the gas guiding groove 13 extends through the length of the hanging plate 11.

[0029] Based on the above structure, the hanging plate 11 can be quickly and conveniently hung on the insertion port 10 by inserting it downwards. The T-shaped structure enables rapid suspension. The hanging plate 11 is inserted into the well-clothed reaction system. The arrangement of the scraper does not need to be carefully considered, as long as it is located within the reaction system. Thus, multiple vertically suspended hanging plates 11 are formed inside the reaction system, and all hanging plates 11 are lifted out of the fermenter by a hanging rack.

[0030] During fermentation, the hanging plates 11 provide a large surface area within a limited volume, offering attachment points to increase bacterial cellulose production. The vertically parallel plate structure minimizes flow obstruction. Liquid and bacteria can flow easily between the plates, ensuring nutrient supply and metabolite diffusion. The vertical placement prevents detached particles or bubbles from becoming trapped. Simultaneously, due to the vertical arrangement of the gas guide channels 13, with the upper part above the liquid surface, a preferred path for bubble rise is provided, promoting the upward removal of suspended bubbles within the reaction system. Especially after a cellulose film is attached to the surface of the hanging plates 11, the grooves prevent large-scale bubble accumulation under the film. This solves the dual problems of degassing and increased production capacity.

[0031] Regarding the design of the gas guide groove 13, since the top of the hanging plate 11 is attached near the tank opening, the top of the gas guide groove 13 is positioned above the liquid surface. This is because the gas metabolism of FY-07 during fermentation is not linear but a complex process. Initially, gas production is very low, and the cells are in the stage of adapting to the environment and preparing for metabolism. Towards the middle stage of fermentation, the number of cells increases exponentially, and metabolism is extremely vigorous. Gas production increases logarithmically, releasing large amounts of carbon dioxide. At this time, cellulose is just beginning to be produced, and the large amount of gas produced can be discharged upwards through the gas guide groove 13, reducing its retention below the liquid surface. When product synthesis stabilizes, the metabolic direction of the cells shifts from "growth" to "product synthesis" (i.e., large-scale production of bacterial cellulose), gas production decreases, the hanging plate 11 is adsorbed by cellulose, and the grooves prevent large-area accumulation of gas bubbles under the membrane.

[0032] Example 2:

[0033] The main structure of this example includes a tank body 1, a tank opening, a tank cover, a mounting ring 2, and a hanger, which are similar to those in Example 1. The difference lies in the structure of the hanger and the layout of the hanging plate 11.

[0034] The mounting ring 2 can be fixed inside the tank body 1 by welding.

[0035] The outer diameter of the annular component 6 of the hanger is larger than the inner diameter of the annular boss 4. The inner circle of the annular boss 4 is symmetrically provided with two L-shaped locking blocks 8 along its circumference. The locking blocks 8 extend upward and the maximum distance between the two locking blocks 8 is greater than the inner diameter of the annular component 6. The inner side of the annular component 6 is provided with a relief groove 7. The relief groove 7 can allow the annular component 6 to fall on the annular boss 4 through the locking blocks 8. By rotating the hanger, it is locked by the locking blocks 8 to obtain a stable limit.

[0036] Multiple sets of baffles 9 are fixed above the annular component 6, arranged in a circular array. The outer ends of the baffles 9 extend downwards and are fixed to the annular component 6, leaving space for the height of the locking block 8. Each set of baffles 9 has a through-hole 10 in the middle, and a hanging plate 11 is movably inserted into the through-hole 10. The top side of the scraper is provided with a baffle 12 in a T-shape with it. Multiple air guide grooves 13 are provided on the two sides of the hanging plate 11. The length of the air guide grooves 13 is adapted to the length of the hanging plate 11, and the air guide grooves 13 are equidistantly arranged along the width direction of the hanging plate 11.

[0037] The hanging plates 11, suspended vertically in a radial pattern within the reaction system, facilitate cellulose adsorption and growth, while their radial distribution also promotes material flow and exchange, making it more suitable for dynamic fermentation.

[0038] If fermentation requires a stirring environment, a coaxial inner ring 14 is provided in the center of the annular component 6, and the inner ends of the baffles 9 are all fixed on the inner ring 14. The inner ring 14 provides clearance for the shaft of the stirring element.

[0039] Not limited to the examples above, in Embodiments 1 and 2, an agitator can also be installed at the bottom of the tank 1 without affecting the setting of the internal mounting plates 11. The agitator can be an axial flow impeller (airfoil impeller), installed at the bottom of the tank below the mounting plates 11, to obtain an axial flow circulating vertically, achieving the most efficient mixing and lowest shear. The fluid is forced to flow evenly through the gaps between each mounting plate 11. This brings fresh oxygen and nutrients to the attached microorganisms while removing CO2 and metabolic waste. The fluid carries bubbles upwards; as it passes through the array of mounting plates 11, large bubbles are broken up, increasing the gas-liquid mass transfer area.

Claims

1. A culture tank for an FY-07 deep fermentation system, characterized in that, The device includes a tank body, the upper part of which is provided with an annular boss that is fixedly installed thereon. A bracket is supported on the annular boss. The bracket includes multiple sets of baffles. Each baffle has a through-hole. A hanging plate is movably inserted into the through-hole. The top side of the hanging plate is provided with a baffle that cannot pass through the through-hole. The bottom side of the hanging plate is located in the middle or lower part of the tank body.

2. The FY-07 submerged fermentation system culture tank according to claim 1, characterized in that, The bracket includes a ring-shaped component, the outer diameter of which is larger than the inner diameter of the ring-shaped boss, and the stop bar is fixed to the ring-shaped component.

3. The FY-07 submerged fermentation system culture tank according to claim 2, characterized in that, The annular boss has a mounting ring fixedly connected to it on its outer periphery. The mounting ring is cylindrical and its outer diameter is adapted to the inner diameter of the tank. Multiple bolts pass through the side wall of the mounting ring and fix the mounting ring to the tank through the bolts. The bolt heads protrude and are exposed inside the mounting ring. The annular boss has equidistant relief grooves on its circumference, and the relief grooves correspond to the spacing and number of bolts.

4. The FY-07 deep fermentation system culture tank according to claim 2, characterized in that, The inner ring of the annular boss has two L-shaped locking blocks symmetrically arranged along its circumference. The locking blocks extend upward and the maximum distance between the two locking blocks is greater than the inner diameter of the annular part. The inner side of the annular part has two or four relief grooves circumferentially arranged. The relief grooves can allow the annular part to fall onto the annular boss through the locking blocks.

5. The FY-07 submerged fermentation system culture tank according to claim 2, characterized in that, The baffles are arranged at equal intervals on the annular component.

6. The FY-07 submerged fermentation system culture tank according to claim 2, characterized in that, The baffles are arranged in a circular array on the ring-shaped component.

7. The FY-07 submerged fermentation system culture tank according to claim 6, characterized in that, The annular component has a coaxial inner ring component at its center, and the inner ends of the baffles are all fixed to the inner ring component.

8. The FY-07 submerged fermentation system culture tank according to claim 1, characterized in that, The mounting plate is provided with an air guide groove along its length, and the length of the air guide groove extends through the length of the mounting plate.

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