reagent tube

Abstract

The utility model provides a reagent tube, include: main pipe, puncture column, limit in the main pipe, amplification pipe, with the main pipe swing joint, be provided with sealing membrane on the amplification pipe, the main pipe is provided with the first limit slot and the second limit slot of amplification pipe's location, the puncture tip interval arrangement of sealing membrane and puncture column of limit in the first limit slot, puncture tip is used for extending into amplification pipe in the second limit slot limit in. The utility model discloses through the cooperation of main pipe, puncture column and amplification pipe, only need to adopt the relative displacement of the external force drive main pipe and amplification pipe between generation, can complete pipetting in reagent tube, simple operation has improved pipetting efficiency, can improve nucleic acid detection precision, can under the drive of external force, make amplification pipe relative puncture column movement, make sealing membrane puncture after, can quantitative transfer liquid to amplification pipe.

Description

Technical Field

[0001] This utility model belongs to the field of nucleic acid testing consumables technology, and in particular relates to a reagent tube. Background Technology

[0002] Rapid, accurate, sensitive, and quantitative detection of specific nucleic acid sequences is playing an increasingly important role in the diagnosis of human infectious diseases, food security, pathogen identification, global biosecurity, and in environmental analysis, as well as in tracking biocontamination and monitoring environmental quality.

[0003] The existing nucleic acid testing process requires mixing samples and various reagents to obtain the nucleic acid to be tested. During the mixing process, the reagents or samples need to be transferred in various consumable tubes. The liquid transfer process is prone to contamination, resulting in impure nucleic acid and inaccurate test results. Furthermore, the liquid transfer process is relatively complex and cumbersome, which makes the nucleic acid testing efficiency low and the testing time long. Utility Model Content

[0004] The main purpose of this invention is to propose a reagent tube that aims to solve the technical problems of low accuracy and long detection time in the existing nucleic acid detection technology.

[0005] To achieve the above objectives, this utility model provides a reagent tube, the reagent tube comprising: a main tube; a piercing column confined within the main tube; and an amplification tube movably connected to the main tube. The amplification tube is provided with a sealing membrane, and the main tube is provided with a first limiting groove and a second limiting groove for limiting the amplification tube. The sealing membrane confined within the first limiting groove and the piercing tip of the piercing column are spaced apart, and the piercing tip is used to extend into the amplification tube confined within the second limiting groove.

[0006] In this embodiment of the present invention, the reagent tube further includes a top cap, which is sealed to the end of the main tube away from the amplification tube. The top cap and the puncture column are integrally formed, or the top cap and the puncture column are separately provided, and the top cap is used to abut against and limit the puncture column.

[0007] In this embodiment of the utility model, the top cover includes: an outer cover body, which is threadedly connected to the main pipe; a limiting protrusion, wherein the outer cover body is provided with a receiving cavity, the limiting protrusion is integrally formed with the outer cover body and located in the receiving cavity, and the limiting protrusion is used to extend into the main pipe and limit the piercing post.

[0008] In this embodiment of the present invention, the limiting protrusion is provided with a first abutting plane that abuts against the piercing post, and the piercing post is provided with a second abutting plane that abuts against the limiting protrusion. The size of the first abutting plane is larger than the size of the second abutting plane.

[0009] In this embodiment of the present invention, the cross-sectional width of the first limiting groove is greater than the cross-sectional width of the second limiting groove, the amplification tube is provided with a hard rubber protrusion that locks with the first limiting groove, and the amplification tube is provided with a soft rubber protrusion that limits the second limiting groove.

[0010] In this embodiment of the invention, the amplification tube is provided with a sealing soft adhesive, and the amplification tube is sealed and connected to the main tube through the sealing soft adhesive.

[0011] In this embodiment of the utility model, the main tube includes: a main body portion having a main chamber; a connecting portion having a limiting chamber communicating with the main chamber and accommodating the amplification tube, wherein the first limiting groove and the second limiting groove are both disposed on the side wall of the limiting chamber, and the piercing column extends from the main chamber into the limiting chamber, with the top of the amplification tube, which is limited by the second limiting groove, abutting against the top wall of the limiting chamber.

[0012] In this embodiment of the invention, the cross-sectional dimensions of the limiting chamber are gradually increased from the second limiting groove to the first limiting groove.

[0013] In this embodiment of the invention, both the first limiting groove and the second limiting groove are annular grooves.

[0014] In this embodiment of the invention, the reagent tube further includes a light-shielding outer tube detachably connected to the main tube, and the light-shielding outer tube is sleeved over the amplification tube.

[0015] Through the above technical solution, the reagent tube provided by this utility model embodiment has the following beneficial effects: The amplification tube can be connected to the lower end of the main tube. The amplification tube, confined in the first limiting groove, is in its initial position. The sealing membrane at the top of the amplification tube and the piercing tip of the piercing column are spaced apart. The sealing membrane does not contact the piercing tip of the piercing column, thus isolating the amplification tube and the main tube from each other. This prevents contact between the contents of the main tube and the contents of the amplification tube, allowing for normal storage separately. When pipetting is required, external mechanical force can drive the main tube and the amplification tube to move relative to each other. The amplification tube can be moved upward while keeping the main tube fixed, or the main tube can be moved downward while keeping the amplification tube fixed, until the amplification tube is confined in the second limiting groove. The piercing tip can pierce the sealing membrane and extend into the amplification tube confined in the second limiting groove, connecting the amplification tube and the main tube. The contents of the main tube and the amplification tube can be mixed, avoiding the need for opening reagent tubes and pipetting operations using external components. The reagent tube of this invention features a simple structure with a main tube, a puncture column, and an amplification tube working together. This facilitates liquid transfer and avoids the need for external components to draw and open the cap to transfer the solution, as required in existing technologies. Only external force is needed to drive relative displacement between the main tube and the amplification tube to complete liquid transfer within the reagent tube. This simplifies operation, improves transfer efficiency, and prevents contamination of the reagent tube contents by the external environment, thus enhancing the accuracy of nucleic acid detection. Under external force, the amplification tube moves relative to the puncture column. With a fixed travel distance between the first and second limiting grooves, the reagent tube remains sealed relative to the outside world. The volume of compressed gas between the amplification tube and the main tube is precisely measured, allowing for the quantitative transfer of liquid into the amplification tube after the sealing membrane is punctured.

[0016] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0017] The accompanying drawings are provided to illustrate the present invention and form part of the specification. They are used together with the following detailed description to explain the present invention, but do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the reagent tube according to one embodiment of the present invention; Figure 2 This is an exploded structural diagram of the reagent tube according to one embodiment of the present invention; Figure 3 This is a schematic diagram of the cross-sectional structure of the reagent tube according to one embodiment of the present invention; Figure 4 This is a schematic diagram of the reagent tube according to one embodiment of the present invention; Figure 5 This is a schematic diagram of the state changes of the reagent tube according to an embodiment of the present invention.

[0018] Explanation of reference numerals in the attached figures Detailed Implementation

[0019] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0020] The reagent tube according to this utility model is described below with reference to the accompanying drawings.

[0021] like Figures 1 to 5 As shown in the embodiment of this utility model, the reagent tube 100 includes a main tube 1, a puncture column 2, and an amplification tube 3; the puncture column 2 is confined within the main tube 1; the amplification tube 3 and the main tube 1 are movably connected, and a sealing membrane is provided on the amplification tube 3. The main tube 1 is provided with a first limiting groove 11 and a second limiting groove 12 that limits the amplification tube 3. The sealing membrane confined in the first limiting groove 11 and the puncture tip of the puncture column 2 are spaced apart, and the puncture tip is used to extend into the amplification tube 3 confined in the second limiting groove 12.

[0022] Understandably, the reagent tube 100 in this embodiment is mainly used for nucleic acid detection. The main tube 1 can contain one solution, and the amplification tube 3 can contain another solution or lyophilized bulbs.

[0023] When using the reagent tube 100 in this embodiment for liquid transfer, the amplification tube 3 can be connected to the lower end of the main tube 1. The amplification tube 3, located in the first limiting groove 11, is in its initial position. The sealing film at the top of the amplification tube 3 and the piercing tip of the piercing column 2 are spaced apart, and the sealing film does not contact the piercing tip of the piercing column 2. The amplification tube 3 and the main tube 1 are isolated from each other, preventing contact between the contents of the main tube 1 and the contents of the amplification tube 3. They can be stored normally separately. When pipetting is required, the main tube 1 and the amplification tube 3 can be driven by external mechanical force. The relative movement between the tube and the main tube allows the amplification tube 3 to move upwards while keeping the main tube 1 fixed, or downwards while keeping the amplification tube 3 fixed, until the amplification tube 3 is confined to the second limiting groove 12. The amplification tube 3 is in its final position relative to the main tube 1. The piercing tip can pierce the sealing film and extend into the amplification tube 3 confined to the second limiting groove 12, connecting the amplification tube 3 and the main tube 1. The contents of the main tube 1 and the amplification tube 3 can be mixed, avoiding the need for opening the reagent tube 100 and pipetting operations using external components. In this embodiment, the reagent tube 100, with its simple structure and convenient pipetting operation through the cooperation of the main tube 1, piercing column 2, and amplification tube 3, avoids the liquid transfer operations of external components drawing and opening the cap to transfer solutions in existing technologies. Only external force is needed to drive relative displacement between the main tube 1 and the amplification tube 3 to complete pipetting within the reagent tube 100. This simple operation improves pipetting efficiency and prevents contamination of the contents of the reagent tube 100 by the external environment, thus improving the accuracy of nucleic acid detection. In this embodiment, under the drive of external force, the amplification tube 3 can move relative to the puncture column 2. With the movement stroke between the first limiting groove 11 and the second limiting groove 12 fixed, the reagent tube 100 is in a sealed state relative to the outside world. The volume of the compressed gas between the amplification tube 3 and the main tube 1 is quantitative, so that after the sealing film is punctured, liquid can be quantitatively transferred into the amplification tube 3.

[0024] like Figures 1 to 3 As shown, the reagent tube 100 also includes a top cap 4, which is sealed to the end of the main tube 1 away from the amplification tube 3. In one embodiment, the top cap 4 and the puncture column 2 are integrally formed; in another embodiment, the top cap 4 and the puncture column 2 are separately arranged, with the top cap 4 used to abut against and limit the puncture column 2. The top cap 4 is located at the top of the main tube 1, and the amplification tube 3 is located at the bottom of the main tube 1. The top of the main tube 1 has an opening that can be sealed by the top cap 4. When the top cap 4 is removed, it is convenient to add samples or liquids to the main tube 1 through the opening. The top cap 4 can also limit the puncture column 2 axially, so that the top cap 4 can seal the main tube 1 while limiting the puncture column 2, preventing the puncture column 2 from moving relative to the main tube 1 under external force. This ensures the accuracy of the liquid transfer operation of the reagent tube 100. The integral formation of the top cap 4 and the puncture column 2 improves manufacturing efficiency.

[0025] It should be noted that the top cover 4 includes an outer cover body 41 and a limiting protrusion 42, with the outer cover body 41 threadedly connected to the main pipe 1. The outer cover body 41 has a receiving cavity, and the limiting protrusion 42 is integrally formed with the outer cover body 41 and located within the receiving cavity. In one embodiment, the piercing post 2 and the limiting protrusion 42 are integrally formed; in another embodiment, the limiting protrusion 42 and the piercing post 2 are separately provided, with the limiting protrusion 42 used to extend into the main pipe 1 and abut against the limiting piercing post 2. In this embodiment, the outer cover body 41 has an internal thread, and the top of the main pipe 1 has an external thread. The main pipe 1 and the outer cover body 41 are connected by a threaded seal and are detachable, resulting in a simple structure and convenient operation. The limiting protrusion 42 is integrally formed with the outer cover body 41, facilitating the production of the top cover 4. The cross-sectional dimension of the limiting protrusion 42 is smaller than the cross-sectional dimension of the inner cavity of the main pipe 1, facilitating the insertion of the limiting protrusion 42.

[0026] In one embodiment, the limiting protrusion 42 is provided with a first abutting surface 421 that abuts against the piercing post 2, and the piercing post 2 is provided with a second abutting surface 21 that abuts against the limiting protrusion 42. The size of the first abutting surface 421 is larger than the size of the second abutting surface 21. Figure 3 As shown, the lower end of the limiting protrusion 42 is provided with a first abutting surface 421, and the top of the piercing post 2 is provided with a second abutting surface 21. The second abutting surface 21 abuts against the middle position of the first abutting surface 421. The size of the first abutting surface 421 is larger than that of the second abutting surface 21, which can ensure the abutting stability between the limiting protrusion 42 and the piercing post 2. In this embodiment, the limiting protrusion 42 is a hollow column. In other embodiments, the limiting protrusion 42 can adopt a reinforcing rib structure, which can strengthen the top cover 4 while limiting the piercing post 2. It should be noted that in one embodiment, the middle part of the piercing post 2 is provided with a column body to accommodate the adsorption ball. The column body is provided with a through hole, the top of the piercing post 2 is provided with a sealing plug, and the bottom of the piercing post 2 is provided with a piercing tip.

[0027] It should be noted that the cross-sectional width of the first limiting groove 11 is greater than that of the second limiting groove 12. The amplification tube 3 is provided with a hard rubber protrusion 32 that locks with the first limiting groove 11, and a soft rubber protrusion 33 that limits the second limiting groove 12. The main tube 1 and the amplification tube 3 are in the initial position relative to each other: the first limiting groove 11 on the main tube 1 acts as a large clamp to hold the bottom hard rubber protrusion 32 of the amplification tube 3, and is used to fix it in place during transportation, etc. The main tube 1 and the amplification tube 3 are in the working position relative to each other: the second limiting groove 12 on the main tube 1 acts as a small clamp to hold the top soft rubber protrusion 33 of the amplification tube 3, and contacts the piercing post 2 to pierce it.

[0028] Understandably, the amplification tube 3 and the main tube 1 are sealed together. In this embodiment, the amplification tube 3 and the main tube 1 are always sealed together, preventing leakage of the reagent tube 100 from the connection between the amplification tube 3 and the main tube 1, and ensuring that the solution inside the reagent tube 100 is not contaminated by the external environment. Figure 3 As shown, the amplification tube 3 is externally covered with a sealing soft adhesive 31, and the amplification tube 3 is sealed to the main tube 1 through the sealing soft adhesive 31. In this embodiment, the sealing connection using the sealing soft adhesive 31 is simple in structure and easy to assemble. Under external force, relative displacement can also occur between the main tube 1 and the amplification tube 3. In other embodiments, a sealing ring can be used to seal the connection between the main tube 1 and the amplification tube 3; the number of sealing rings can be set according to actual usage requirements. The amplification tube 3 has two soft adhesive protrusions 33, which can maintain a sealed state.

[0029] like Figure 4 As shown, the main tube 1 includes a main body 13 and a connecting part 14. The main body 13 is provided with a main chamber 131; the connecting part 14 is provided with a limiting chamber 141 that communicates with the main chamber 131 and accommodates the amplification tube 3. A first limiting groove 11 and a second limiting groove 12 are both provided on the side wall of the limiting chamber 141. The piercing column 2 extends from the main chamber 131 into the limiting chamber 141, and the top of the amplification tube 3, which is limited by the second limiting groove 12, abuts against the top wall of the limiting chamber 141. In this embodiment, the main chamber 131 is located at the top of the limiting chamber 141, the first limiting groove 11 is provided near the bottom of the connecting part 14, and the second limiting groove 12 is provided near the top of the connecting part 14, as shown. Figure 5 As shown, the amplification tube 3 on the left is limited to the first limiting groove 11, and the amplification tube 3 on the right is limited to the second limiting groove 12. While the amplification tube 3 and the second limiting groove 12 cooperate in circumferential limiting, the top wall of the limiting chamber 141 limits the axial direction of the amplification tube 3, which can ensure that the amplification tube 3 accurately reaches the designated position under the action of external force, and ensure the limiting stability between the main tube 1 and the amplification tube 3.

[0030] In one embodiment, the first limiting groove 11 and the second limiting groove 12 can both limit the protrusion on the sealing soft rubber 31. In another embodiment, the first limiting groove 11 and the second limiting groove 12 can both limit the tube protrusion below the sealing soft rubber 31 on the amplification tube 3. In yet another embodiment, the first limiting groove 11 limits the tube protrusion, and the second limiting groove 12 limits the protrusion on the sealing soft rubber 31.

[0031] Specifically, the cross-sectional dimensions of the limiting chamber 141 gradually increase from the second limiting groove 12 towards the first limiting groove 11. For example... Figures 3 to 5As shown, in this embodiment, the wall thickness of the limiting chamber 141 gradually increases from bottom to top, ensuring a tight fit between the amplification tube 3 and the main tube 1, which are confined within the second limiting groove 12. Furthermore, the cross-sectional dimensions of the limiting chamber 141 gradually expand from the second limiting groove 12 towards the first limiting groove 11, guiding the amplification tube 3 and facilitating its entry into the limiting chamber 141. The varying slopes at the bottom of the limiting chamber 141 facilitate the insertion of the amplification tube 3, slightly widening it to allow for a gap while ensuring a secure hold.

[0032] It should be noted that both the first limiting groove 11 and the second limiting groove 12 are annular grooves. In this embodiment, the first limiting groove 11 is arranged around the side wall of the limiting chamber 141, and the second limiting groove 12 is arranged around the side wall of the limiting chamber 141, so that the first limiting groove 11 and the second limiting groove 12 can circumferentially limit the amplification tube 3, ensuring the limiting contact area between the main tube 1 and the amplification tube 3.

[0033] like Figure 2 As shown, the reagent tube 100 also includes a light-shielding outer tube 5 detachably connected to the main tube 1, and the light-shielding outer tube 5 is sleeved around the amplification tube 3. In this embodiment, the light-shielding outer tube 5 and the main tube 1 can be snapped together. The light-shielding outer tube 5 is arranged around the amplification tube 3 to protect the amplification tube 3 from light exposure, thereby preventing the contents of the amplification tube 3 from becoming ineffective due to light exposure, thus ensuring the stability of the reagents stored in the reagent tube 100.

[0034] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0035] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0036] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0037] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A reagent tube, characterized in that, The reagent tube (100) includes: Supervisor (1); Pierce the column (2), confining it within the main tube (1); An amplification tube (3) is movably connected to the main tube (1). A sealing membrane is provided on the amplification tube (3). The main tube (1) is provided with a first limiting groove (11) and a second limiting groove (12) that limit the amplification tube (3). The sealing membrane located in the first limiting groove (11) and the piercing tip of the piercing column (2) are spaced apart. The piercing tip is used to extend into the amplification tube (3) located in the second limiting groove (12).

2. The reagent tube according to claim 1, characterized in that, The reagent tube (100) also includes a top cover (4), which is sealed to the end of the main tube (1) away from the amplification tube (3). The top cover (4) and the puncture column (2) are integrally formed, or the top cover (4) and the puncture column (2) are separately set. The top cover (4) is used to abut and limit the puncture column (2).

3. The reagent tube according to claim 2, characterized in that, The top cover (4) includes: The outer cover (41) is threadedly connected to the main tube (1); The limiting protrusion (42) and the outer cover (41) are provided with a receiving cavity. The limiting protrusion (42) and the outer cover (41) are integrally formed and located in the receiving cavity. The limiting protrusion (42) is used to extend into the main tube and limit the piercing column (2).

4. The reagent tube according to claim 3, characterized in that, The limiting protrusion (42) is provided with a first abutting surface (421) that abuts against the piercing post (2), and the piercing post (2) is provided with a second abutting surface (21) that abuts against the limiting protrusion (42). The size of the first abutting surface (421) is larger than the size of the second abutting surface (21).

5. The reagent tube according to any one of claims 1 to 4, characterized in that, The cross-sectional width of the first limiting groove (11) is greater than the cross-sectional width of the second limiting groove (12). The amplification tube (3) is provided with a hard rubber protrusion (32) that locks with the first limiting groove (11), and the amplification tube (3) is provided with a soft rubber protrusion (33) that limits the second limiting groove (12).

6. The reagent tube according to any one of claims 1 to 4, characterized in that, The amplification tube (3) is provided with a sealing soft rubber (31) on the outside, and the amplification tube (3) is sealed and connected to the main tube (1) through the sealing soft rubber (31).

7. The reagent tube according to any one of claims 1 to 4, characterized in that, The supervisor (1) includes: The main body (13) is provided with a main chamber (131); The connecting part (14) is provided with a limiting chamber (141) that communicates with the main chamber (131) and accommodates the amplification tube (3). The first limiting groove (11) and the second limiting groove (12) are both provided on the side wall of the limiting chamber (141). The piercing column (2) extends from the main chamber (131) into the limiting chamber (141) and the top of the amplification tube (3) limited by the second limiting groove (12) abuts against the top wall of the limiting chamber (141).

8. The reagent tube according to claim 7, characterized in that, The cross-sectional dimensions of the limiting chamber (141) gradually expand from the second limiting groove (12) towards the first limiting groove (11).

9. The reagent tube according to any one of claims 1 to 4, characterized in that, Both the first limiting groove (11) and the second limiting groove (12) are annular grooves.

10. The reagent tube according to any one of claims 1 to 4, characterized in that, The reagent tube (100) also includes a light-shielding outer tube (5) that is detachably connected to the main tube (1), and the light-shielding outer tube (5) is sleeved on the outside of the amplification tube (3).

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