Veterinary formulation, including CBD and / or THC for use in the treatment of stress in bees

Description

Technical Field

[0001] The field of art to which this invention generally pertains is veterinary compositions, and specifically treatment methods and compositions for bees.Background

[0002] The productivity of bees can be very sensitive to such things as viruses, stress, etc. This can show itself in many ways, such as, for example, amount of honey produced per a particular bee hive. Accordingly, there is a constant search in this area for ways to address these issues.

[0003] PCT Publication No. WO 2017 / 072704 discloses therapeutic compositions comprising honey, at least one cannabinoid, and water at a concentration of less than 26%wt. PCT Publication No. WO 2017 / 013661 discloses a bee ingestible composition comprising a bee food base and THC and / or CBD. PCT Publication No. WO 2015 / 143536 discloses honey mite disruptive compounds. PCT Publication No. WO 97 / 47193 discloses a method for the control of infestations of honeybee colonies by applying to the bee-hives an effective amount of an essential oil in a slow-release formulation.Brief Summary

[0004] According to an aspect of the present invention there is provided a veterinary composition comprising at least one cannabinoid comprising CBD and / or THC, optionally an extract of cannabis plant material; for use in the treatment of stress in bees at or near a bee hive.

[0005] According to some embodiments, the veterinary composition additionally contains an additive selected from the group consisting of emulsifiers, antioxidants, sugars, pollen, pollen substitutes, pollen supplements, omega-3 fatty acids, vegetable oil, minerals, vitamins, fats, essential oils, invertase enzyme and combinations thereof.

[0006] According to some embodiments, the veterinary composition additionally contains an additive selected from the group consisting of compounds characteristic to eucalyptus flowers, compounds characteristic to avocado flowers, compounds characteristic to orange blossom, compounds characteristic to wild flowers, characteristic to Tribulus terrestris and combinations thereof.

[0007] According to some embodiments, the veterinary composition is for administering by vaporizing the composition in the beehive, spraying the composition into the atmosphere of the beehive, spraying the composition on honeycombs in the beehive, spraying the composition on brood-containing honeycombs, incorporating the composition into beeswax used to form the beehive frames or combination thereof.

[0008] According to some embodiments, the veterinary composition is for administering to the bees when present at or near a beehive containing brood cells, wherein the count of brood cells in said beehive, the count of sealed brood cells in said beehive or both is at least 10% greater than those in a beehive under the same conditions receiving no veterinary composition treatment.

[0009] According to some embodiments, the composition is for administration to bees during winter.

[0010] According to some embodiments, the composition is contained in an aerosol, spray and / or vapor.

[0011] A description of these embodiments is included in greater detail below.Definitions

[0012] Unless specified otherwise, all concentrations are weight concentrations and all ratios are weight per weight (weight / weight) ratios.

[0013] As used herein, the term "cannabinoid(s)" refers to both cannabinoid(s) in carboxylic acid form and cannabinoids in decarboxylated form. The same is true for each cannabinoid, e.g. THC and CBD.

[0014] As used herein, the term "non-cannabinoid cannabis compound" refers to a non-cannabinoid compound present in at least one strain of cannabis plants.

[0015] As used herein the term "terpene" refers to compounds comprising at least one isoprene unit. The terms "terpene" and "terpenoid" are used herein interchangeably.

[0016] As used herein the term "terpene other than cannabis terpenes" refers to a terpene that is not common to or typically doesn't exist in cannabis plants.

[0017] As used herein the term "flavonoid other than cannabis flavonoids" refers to a flavonoid that is not common to or typically doesn't exist in cannabis plants.

[0018] As used herein the term "extract of a non-cannabis plant" refers to any product of extracting a plant other than a cannabis plant.

[0019] As used herein, the term carrier refers to any non-cannabis compound.

[0020] As used herein, the term non-cannabis compound refers to compounds not present in cannabis plant or present there at concentration of less than 5%.

[0021] As used herein, the term cannabis plant material refers to material derived from a cannabis plant.

[0022] As used herein, the terms pollen, pollen substitutes and pollen supplements refer to protein-containing feed ingredients, whether containing flower pollen or not.

[0023] As used herein, the terms "honeycomb", "honeycomb frames", and "frames" are interchangeable.

[0024] As used herein, the terms administering a composition to bees refers to dispensing or applying the composition in a way that makes the composition available to bees, to bees brood and / or to bees pathogens.

[0025] As used herein, invert sugar and inverted sugar refer to the product of sucrose hydrolysis (into glucose and fructose) e.g. the result of treating sucrose with the enzyme invertase.

[0026] As used herein the term honey refers to concentrated sugar compositions referred to commercially as honey and excludes cannabis extract as such. As used herein, the term a comprising honey refers to comprising components of commercial pure honey (sugars, amino acids, organic acids, furfurals, vitamins, minerals).

[0027] As used herein, the term commercial pure honey refers to unadulterated honey sold globally at a rate of at least one ton per year.

[0028] As used herein, the term excipient refers to a compound used to formulate an active ingredient.Detailed Description of the Invention

[0029] Provided is a veterinary composition comprising at least one cannabinoid comprising CBD and / or THC, and optionally an extract of cannabis plant material, for use in the treatment of bees at or near a bee hive.

[0030] Any form of administering and any combination of the veterinary composition with other ingredients are suitable. According to an embodiment, treating comprises administering, dispensing or supplying said veterinary composition to the bees in combination with bees feed, or with bees feed additives or with both. As use herein, the term in combination with refers to incorporated in, blended with or administering concurrently with.

[0031] For example, in many cases, bees keepers feed the bees sugar solution as an energy source during the time when nectar supply is insufficient to support the activity, e.g. before spring starts. Typically, the solutions are of 50-60% sugar and contain, sucrose, glucose, fructose, high-fructose corn syrup, inverted sugar or combinations thereof. According to an embodiment, the veterinary composition is blended with the sugar solution before feeding it to the bees or during feeding. According to an embodiment, said veterinary composition is administered in combination with each one of the sugar feeding. According to an embodiment, said veterinary composition is administered in combination with each one of the sugar feeding, whereby the veterinary composition is evenly consumed and / or evenly enhancing performance in the bee hive.

[0032] Alternatively, or additionally bees keepers feed bees with a protein ingredient, such as pollen, pollen substitutes, pollen supplements. According to an embodiment, the veterinary composition is blended with the protein ingredient before feeding it to the bees or during feeding. Any source of pollen is suitable, including cannabis pollen. According to an embodiment, said veterinary composition is administered in combination with each one of the protein feeding. According to an embodiment, said veterinary composition is administered in combination with each one of the protein feeding, whereby the veterinary composition is evenly consumed and / or evenly enhancing performance in the bee hive.

[0033] According to an embodiment, administering the veterinary composition comprises vaporizing the composition in the beehive, spraying the composition into the atmosphere of the beehive, spraying the composition on honeycombs in the beehive, spraying the composition on brood-containing honeycombs, incorporating the composition into beeswax used to form the beehive frames or combination thereof.

[0034] The boiling points of cannabinoids and of non-cannabinoid cannabis compounds, such as terpenes, typically range between about 100°C and about 200°C. Heating to this temperature range (or to a lower temperature at sub-atmospheric pressure and / or on using a carrier gas) leads to the formation of suitable vapors to be administered to the bees. Vaporizers similar to those used for administering medical cannabis to patients can be used to form such vapors.

[0035] According to an embodiment, said veterinary composition is administered to the bees between 2 and 12 times per year, between 2 and 10, between 3 and 9, between 3 and 8 or between 3 and 6. According to an embodiment, said veterinary composition is administered to the bees during winter, during spring or within several weeks (e.g. within 4-6 weeks) before blooming start is predicted. According to an embodiment, said veterinary composition is administered to the bees during shortage in food, during times of negative energy balance in the hive (more energy is used than provided) during high pathogens content, during times when ambient temperature is lower than 25°C, lower than 20°C lower than 15°C, or lower than 10°C or during times when ambient temperature is higher than 35°C.

[0036] According to an embodiment, treating comprises administering to the bees between 1 milligram (mg) and 1000 milligram veterinary composition per beehive, between 1.5mg and 400mg, between 2mg and 300mg or between 2.5mg and 200mg. According to an embodiment, treating comprises administering to the bees between 0.1mg and 300mg cannabinoid per bee hive, between 0.2mg and 200mg, between 0.3mg and 150mg or between 0.5mg and 100mg. According to an embodiment, treating comprises administering to the bees between 0.1mg and 300mg non-cannabinoid cannabis compounds per bee hive, between 0.2mg and 200mg, between 0.3mg and 150mg or between 0.5mg and 100mg.

[0037] According to an embodiment, said veterinary composition comprises at least two cannabinoids, at least three, at least four or at least five. According to an embodiment, said veterinary composition comprises at least three non-cannabinoid cannabis compounds, at least four, at least five, at least six or at least ten.

[0038] According to various embodiments, said veterinary composition comprises tetrahydrocannabinol (THC), cannabidiol (CBD) or both. According to various embodiment, said veterinary composition comprises both THC and CBD at a weight / weigh ratio between 30:1 and 1:30, between 20:1 and 1:20, or between 10:1 and 1:10.

[0039] According to an embodiment, said veterinary composition includes at least one terpene, at least two, at least three non-cannabinoid cannabis compounds, at least four, at least five, at least six or at least ten. According to an embodiment, said veterinary composition includes at least one flavonoid, at least two, at least three non-cannabinoid cannabis compounds, at least four or at least five.

[0040] According to an embodiment, said non-cannabinoid cannabis compounds comprise non-cannabinoid cannabis compounds characteristic to Sativa cannabis strains and / or to Indica strains. According to an embodiment, said non-cannabinoid cannabis compounds comprise non-cannabinoid cannabis compounds characteristic to Sativa cannabis strains. According to an embodiment, said non-cannabinoid cannabis compounds comprise non-cannabinoid cannabis compounds characteristic to Indica cannabis strains. By characteristic to such strains recited is meant that the compounds are typically contained in each of the strains recited in an amount serving to identify it as associated with such strain, but not exclusively contained in such strain, i.e., may also be present in other of the strains as well.

[0041] According to an embodiment, said veterinary composition comprises an extract of cannabis plant material, e.g. as formed in contacting said plant material with an extractant, and / or as formed by distilling components out of said plant material, e.g. as in steam distillation. Any extractant is suitable. According to an embodiment, said extractant is ethanol, a hydrocarbon, carbon dioxide or a combination thereof.

[0042] According to an embodiment, said veterinary composition additionally contains an emulsifier and / or an antioxidant. According to an embodiment, said veterinary composition, further comprises a compound selected from the group consisting of mono-glycerides, diglycerides, lecithin, gums (e.g. xanthan gum) and polysaccharides. According to an embodiment, said veterinary composition is lipophilic in nature and is administered in a blend with an aqueous solution and said emulsifier is selected to enable homogeneous dispersion of the veterinary composition in said aqueous solution. According to an embodiment, said emulsifier is food approved, biological and / or natural. Optionally, said veterinary composition comprises an antioxidant, whereby said veterinary composition is better protected during prolonged storage.

[0043] According to an embodiment, said veterinary composition additionally contains an additive selected from the group consisting of sugars, pollen, pollen substitutes, pollen supplements, polyunsaturated fatty acids, omega-3 fatty acids from various sources, vegetable oil (e.g. coconut oil), minerals, vitamins, fats, essential oils, naringin, beeswax, invertase enzyme and combinations thereof. According to an embodiment, said veterinary composition additionally contains a pharmaceutical. According to an embodiment, said pharmaceutical comprises antibiotics. According to an embodiment, said pharmaceutical is selected from oxytetracyline and dabicycline.

[0044] According to an embodiment, the content of said at least one cannabinoid reduces the required amount of pharmaceutical compared with veterinary compositions containing the same pharmaceutical, but without inclusion of any cannabinoids. According to an embodiment, said veterinary composition administrated to bees comprise antibiotics at an amount of less than 60mg per beehive, less than 50mg, less than 40mg, less than 30mg or less than 20mg.

[0045] According to an embodiment, said veterinary composition additionally contains an additive selected from the group consisting of compounds characteristic to eucalyptus flowers, compounds characteristic to avocado flowers, compounds characteristic to orange blossom, compounds characteristic to wild flowers, characteristic to Tribulus terrestris and combinations thereof. By characteristic to such flowers and materials recited is meant that the compounds are typically contained in each of the flowers and materials recited in an amount serving to identify it as associated with such flower or material, but not exclusively contained in such material, i.e., may also be present in other of the flowers and materials recited as well.

[0046] According to an embodiment, a carrier comprises at least one of a sugar, honey, beeswax, pollen, pollen substitutes, pollen supplements, a protein ingredient, vegetable oil and fiber. According to an embodiment, said carrier comprises a sugar selected from the group consisting of sucrose, glucose, fructose, high fructose corn syrup, invert sugar and combinations thereof. According to an embodiment, said carrier comprises honey. According to an embodiment, said carrier comprises sugar and honey.

[0047] According to an embodiment, said carrier comprises an aqueous solution of at least one sugar. According to an embodiment, said carrier comprises an aqueous solution of glucose and fructose. According to an embodiment, said carrier comprises an aqueous solution of inverted sugar. According to an embodiment, sugars concentration in said aqueous solution is in the range between 20% and 80, between 30% and 70% or between 45% and 65%,

[0048] According to an embodiment, said carrier comprises solid sugar. According to an embodiment, said carrier comprises solid sugar powder. According to an embodiment, said carrier comprises a protein preparation or protein-containing plant material. According to an embodiment, said carrier comprises a mixture of pollen and sugar.

[0049] According to an embodiment, said veterinary composition is characterized by being homogenous or of consistent composition. According to an embodiment, said at least one cannabinoid is homogeneously dispersed in said carrier. As used herein, homogeneously dispersed means consistently distributed. According to an embodiment, at least two samples of at least about 500mg each, taken from said veterinary second composition have similar concentrations of said at least one cannabinoid, e.g. within 10% difference. According to an embodiment, at least two samples of at least about 500mg each, taken from said veterinary second composition comprising multiple cannabinoids, have similar concentrations of multiple (e.g. at least 2, at least 3, at least 5 or at least 10) cannabinoid, e.g. within 10% difference (e.g. in case said therapeutic composition comprises cannabinoid A and cannabinoid B, the concentration of cannabinoid A in one sample is similar to the concentration of cannabinoid A in another sample and the concentration of cannabinoid B in one sample is similar to the concentration of cannabinoid B in another sample).

[0050] According to an embodiment, said at least two non-cannabinoid cannabis compounds are homogeneously dispersed in said carrier. According to an embodiment, at least two samples of at least about 500mg each, taken from said veterinary second composition have similar concentrations of said at least two non-cannabinoid cannabis compounds, e.g. within 10% difference.

[0051] According to an embodiment, said veterinary composition includes a cannabinoid and the weight ratio between said carrier and said cannabinoid is greater than 5, greater than 8, greater than 15, greater than 20, greater than 25, greater than 30, greater than 40, greater than 50, greater than 60, or greater than 80. According to an embodiment, said veterinary second composition includes non-cannabinoid cannabis compounds and the weight ratio between said carrier and said non-cannabinoid cannabis compounds is greater than 5, greater than 8, greater than 15, greater than 20, greater than 25, greater than 30, greater than 40, greater than 50, greater than 60, or greater than 80.

[0052] According to an embodiment, said veterinary composition comprises at least 0.001% by weight cannabinoid, at least 0.001% by weight non-cannabinoid cannabis compounds and at least 40% by weight carrier. According to an embodiment, it comprises at least 0.001% by weight cannabinoid, at least 0.002%, at least 0.004%, at least 0.006%, at least 0.008%, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, at least 0.06%, at least 0.08%, at least 0.1%, at least 0.2%, at least 0.4%, at least 0.6%, at least 0.8% or at least 1%. According to an embodiment, it comprises less than 10% by weight cannabinoid, less than 8%, less than 6%, less than 4%, less than 2%, less than 1%, less than 0.8% or less than 0.5%. According to an embodiment, said veterinary second composition comprises at least 0.001% by weight non-cannabinoid cannabis compounds, at least 0.002%, at least 0.004%, at least 0.006%, at least 0.008%, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, at least 0.06%, at least 0.08%, at least 0.1%, at least 0.2%, at least 0.4%, at least 0.6%, at least 0.8% or at least 1%. According to an embodiment, it comprises less than 10% by weight non-cannabinoid cannabis compounds, less than 8%, less than 6%, less than 4%, less than 2%, less than 1%, less than 0.8% or less than 0.5%. According to an embodiment, said veterinary second composition comprises at least 45% by weight carrier, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80% or at least 85%.

[0053] According to an embodiment, said veterinary composition includes a cannabinoid and water and the weight ratio between the water and said cannabinoid is greater than 5, greater than 8, greater than 15, greater than 20, greater than 25, greater than 30, greater than 40, greater than 50, greater than 60, or greater than 80.

[0054] According to an embodiment, said veterinary second composition includes non-cannabinoid cannabis compounds and water and the weight ratio between the water and said non-cannabinoid cannabis compounds is greater than 5, greater than 8, greater than 15, greater than 20, greater than 25, greater than 30, greater than 40, greater than 50, greater than 60, or greater than 80.

[0055] According to various embodiments, said veterinary composition comprises at least one cannabinoid and / or at least two non-cannabinoid cannabis compounds, at least 40% by weight sugar and at least 40% by weight water; at least one cannabinoid, at least 40% by weight sugar and at least 40% by weight water; at least two non-cannabinoid cannabis compounds, at least 40% by weight sugar and at least 40% by weight water; or at least one cannabinoid, at least two non-cannabinoid cannabis compounds, at least 40% by weight sugar and at least 40% by weight water.

[0056] According to various embodiments, in said veterinary composition said at least one cannabinoid and / or said at least two non-cannabinoid cannabis compounds are homogeneously dispersed in said carrier; said at least one cannabinoid is homogeneously dispersed in said carrier; said at least two non-cannabinoid cannabis compounds are homogeneously dispersed in said carrier; or said at least one cannabinoid and said at least two non-cannabinoid cannabis compounds are homogeneously dispersed in said carrier.

[0057] According to some embodiments, said veterinary composition comprises at least one cannabinoid and / or at least two non-cannabinoid cannabis compounds and additionally containing pollen, pollen substitutes and / or pollen supplements. Any source of pollen is suitable, including cannabis pollen. According to some embodiments, said veterinary second composition includes at least one cannabinoid and / or at least two non-cannabinoid cannabis compounds and additionally contains 30% protein.

[0058] According to an embodiment, said veterinary composition additionally contains an emulsifier and / or an antioxidant. According to an embodiment, said emulsifier is food approved, biological and / or natural. According to an embodiment, said veterinary second composition, further comprises a compound selected from the group consisting of mono-glycerides, diglycerides, lecithin, gums (e.g. xanthan gum) and polysaccharides. Optionally, said veterinary second composition comprises an antioxidant, whereby said veterinary second composition is better protected during prolonged storage.

[0059] According to an embodiment, said veterinary composition additionally contains an additive selected from the group consisting of sugars, pollen, pollen substitutes, pollen supplements, omega- polyunsaturated fatty acids, 3 fatty acids from various sources, vegetable oil (e.g. coconut oil), minerals, vitamins, fats, essential oils, beeswax, naringin, invertase enzyme, and combinations thereof. According to an embodiment, said veterinary second composition additionally contains a pharmaceutical. According to an embodiment, said pharmaceutical comprises antibiotics. According to an embodiment, said pharmaceutical is selected from oxytetracyline and dabicycline.

[0060] According to an embodiment, said veterinary composition additionally contains an additive, e.g. a terpene, selected from the group consisting of compounds characteristic to eucalyptus flowers, compounds characteristic to avocado flowers, compounds characteristic to orange blossom, compounds characteristic to wild flowers, characteristic to Tribulus terrestris and combinations thereof. By characteristic to such flowers and materials recited is meant that the compounds are typically contained in each of the flowers and materials recited in an amount serving to identify it as associated with such flower or material, but not exclusively contained in such material, i.e., may also be present in other of the flowers and materials recited as well.

[0061] According to an embodiment, said veterinary composition, further comprises at least 10ppm terpene other than cannabis terpenes, at least 50ppm, at least 100ppm, at least 500ppm, at least 1000ppm, at least 3000ppm, at least 5000ppm or at least 10000ppm.

[0062] According to an embodiment, said veterinary composition, further comprises at least one excipient. According to an embodiment, said excipient is said carrier. According to an embodiment, said at least one cannabinoid and at least two non-cannabinoid cannabis compounds are homogeneously dispersed in said carrier and said veterinary second composition comprises said homogeneous dispersion and at least one excipient. According to an embodiment, said excipient coats said homogeneous dispersion.

[0063] According to an embodiment, said veterinary composition is in solid form, in liquid form, in aerosol form, in gel form, in tablet form or in a capsule form. According to an embodiment, further provided is an aerosol comprising said veterinary second composition. According to an embodiment, further provided is a solid preparation comprising said veterinary second composition. According to an embodiment, further provided is a liquid preparation comprising said veterinary second composition.

[0064] According to an embodiment, said veterinary composition is administered to the bees between 2 and 12 times per year, between 2 and 10, between 3 and 9, between 3 and 8 or between 3 and 6. According to an embodiment, said veterinary second composition is administered to the bees during winter, during spring or within several weeks (e.g. within 4-6 weeks) before blooming start is predicted. According to an embodiment, said veterinary second composition is administered to the bees during shortage in food, during times of negative energy balance in the hive (more energy is used than provided) during high pathogens content, during times when ambient temperature is lower than 25°C, lower than 20°C lower than 15°C, or lower than 10°C or during times when ambient temperature is higher than 35°C.Examples Example 1: Preparing cannabis extracts

[0065] Buds of two strains were dried at ambient temperature and at controlled moisture. The dried buds were then ground and mixed well for homogenization. Moisture content was 11.2%wt. The dried and ground plant material was extracted by mixing with 95% ethanol solution at 10 milliliter (ml) ethanol solution per 1 gram of plant material. Mixing was conducted at ambient temperature for 30 minutes. Then the plant material was filtered out and the solution was evaporated at sub-atmospheric pressure for removing the majority of the ethanol. The remaining solution was kept in a freezer for at least 24hours, followed by filtering out waxes. The de-waxed solution was further evaporated for removing the rest of the ethanol. The residual solution was then kept at a temperature of 120°C until bubbling stopped to form the decarboxylated extract. Samples of the extracts were analyzed for their cannabinoids content. The results are summarized in Table 1: Table 1ExtractStrain (source in Israel)CBD(%wt)CBN(%wt)THC(%wt)1CHEESE-PIE (Better)61.12.82QUARTZ (BOL)1.061.3 Example 2: Blending extracts with sucrose solutions

[0066] Food-grade sucrose was mixed with water to form an aqueous solution of 55% sucrose.Comparative method:

[0067] Several trials were made to form homogeneous mixtures where said 55% sucrose solution forms a carrier to deliver the extracts of Example 1. The weight / weight ratios between the extracts and the 55% sucrose solutions were selected to reach cannabinoids concentration in the mixture in the range between 20ppm and 1000ppm. No homogeneous solution was formed. At least a fraction of the extract remained undissolved and tended to stick to the walls of the sugar container.Working method:

[0068] The extract was mixed with honey to form extract-honey mixtures and those extract-honey mixtures were mixed with the 55% sucrose solution. Thus, 30-50 grams honey as such or after dilution with 10% water, 20% water or 30% water, was warmed to 45°C. Then the extract was gradually added, while thoroughly mixing. Mixing was continued for 30 minutes after the addition is completed, whereby extract-honey mixtures were formed. The 55% sucrose solution was gradually added to the extract-honey mixtures until the desired overall cannabinoid concentration was reached. The weight / weight ratio between honey and the sucrose solution were in the range between 1:10 and 1:1000. At all tested cannabinoid concentrations, up to several thousand parts per million, the formed final solutions were homogeneous.Example 3: Production of the veterinary composition

[0069] The working method of Example 2 was used to prepare veterinary compositions containing 55% sucrose, honey and extract. In preparing the veterinary composition, the weight / weight ratio between honey and the sucrose solution was 1:20. Three types of veterinary composition were prepared according to Table 2. Table 2: Veterinary compositionsVeterinary compositionSource [1]Cannabinoid concentration (ppm)THCCBDAExtract 1241BExtract 241CExtract 1 + Extract 220.520.5[1] Source of cannabinoids and of non-cannabinoid cannabis compounds Example 4: Administering the veterinary compositions

[0070] For administering the veterinary compositions to bees at or near a bee hive, freshly prepared veterinary compositions, containing the desired amounts of cannabinoids and of non-cannabinoid cannabis compounds, were mixed with 55% sucrose solution to form feeds. The feeds were provided to the bees in feeding trays. The majority of the feed was consumed within several hours.Example 5: Trial bee hive setting

[0071] Eighteen bee hives were selected and randomly divided into three groups - Group 1, Group 2 and Group 3 - each one containing six hives. All hives were positioned at the same location in a field at Kfar Bilu, Israel.Example 6: Feeds for the various groups

[0072] The groups were fed four times during February and March 2016: Group I received three feeds (1 st< , 3 rd< and 4 th< ) prepared by forming veterinary compositions according to A in Example 3 and mixing them with 55% sucrose solution according to Example 4. The 2 nd< feeding was prepared by forming a veterinary composition according to C in Example 3 and mixing it with 55% sucrose solution according to Example 4. Group 2 received four feeds prepared by forming veterinary compositions according to B in Example 3 and mixing them with 55% sucrose solution according to Example 4. Group 3 (control) received four 55% sucrose solutions. Example 7: Feeding schedule and details

[0073] The feeding and their timing are summarized in Table 3. Table 3: Feeding schedule and detailsGroup 1Group 2Group 31 st< feedingDateFeed amount per hive (Kg)(kilograms)333Cannabinoid per hive3mg CBD3mg THC0Non-cannabinoid [1] per hive2mg2mg02 nd< feedingDateFeed amount per hive (Kg)333Cannabinoid per hive1.5mg CBD + 1.5mg THC3mg THC0Non-cannabinoid [1] per hive2mg2mg03 rd< feedingDateFeed amount per hive (Kg)333Cannabinoid per hive6.7mg CBD6.7mg THC0Non-cannabinoid [1] per hive4.4mg4.4mg04 th< feedingDateFeed amount per hive (Kg)333Cannabinoid per hive6.7mg CBD6.7mg THC0Non-cannabinoid [1] per hive4.4mg4.4mg0[1] Non-cannabinoid cannabis composition Example 8: Number of full honeycombs

[0074] At various dates, all hives were checked for the number of full honeycombs therein. Table 4 summarizes the average number of full honeycombs per hive at the checking dates. Table 4: Average number of full honeycombs per hiveFull honeycombs per hiveGroup 1Group 2Group 3DateFeb 5, 20166.25.26Feb 16, 20168.36.47.8March 6, 20169.89.29.7March 14, 20161415.511.3March 22, 20161817.411.3April 5, 201622.421.214.2May 2, 201623.621.318.7

[0075] These number of full honeycombs per hive demonstrate 60% enhancement in performance as a result of administering few milligrams of cannabinoid and / or few milligrams of non-cannabinoid cannabis compounds per bee hive.Example 9: Honey harvest

[0076] Honey was first harvested on May 18, 2016. Second harvest was on August 1, 2016. The average amounts of honey per hive are summarized in Table 5. Table 5: Average amount of harvested honey per hiveHarvested honey per hive (Kg)Group 1Group 2Group 31 st< harvest22.718.114.82 nd< harvest16.618.912.1Total39.337.026.9

[0077] The average amount of harvested honey is another indication of improved performance on administering few milligrams of cannabinoid and / or few milligrams of non-cannabinoid cannabis compounds per bee hive.

Claims

1. A veterinary composition comprising at least one cannabinoid comprising CBD and / or THC, optionally an extract of cannabis plant material; for use in the treatment of stress in bees at or near a bee hive.

2. The veterinary composition for use according to Claim 1, wherein said veterinary composition additionally contains an additive selected from the group consisting of emulsifiers, antioxidants, sugars, pollen, pollen substitutes, pollen supplements, omega-3 fatty acids, vegetable oil, minerals, vitamins, fats, essential oils, invertase enzyme and combinations thereof.

3. The veterinary composition for use according to Claim 1, wherein said veterinary composition additionally contains an additive selected from the group consisting of compounds characteristic to eucalyptus flowers, compounds characteristic to avocado flowers, compounds characteristic to orange blossom, compounds characteristic to wild flowers, compounds characteristic to Tribulus terrestris and combinations thereof.

4. The veterinary composition for use according to Claim 1, for administering by vaporizing the composition in the beehive, spraying the composition into the atmosphere of the beehive, spraying the composition on honeycombs in the beehive, spraying the composition on brood-containing honeycombs, incorporating the composition into beeswax used to form the beehive frames or combination thereof.

5. The veterinary composition for use according to Claim 1, for administering to the bees when present at or near a beehive containing brood cells, wherein the count of brood cells in said beehive, the count of sealed brood cells in said beehive or both is at least 10% greater than those in a beehive under the same conditions receiving no veterinary composition treatment.

6. The veterinary composition for use according to Claim 1, wherein said composition is for administration to bees during winter.

7. The veterinary composition for use according to Claim 1, wherein said composition is contained in an aerosol, spray and / or vapor.