Detergents and cleaning agents comprising dispersin

Abstract

The invention relates to detergents and cleaning agents, in particular enzyme-containing detergents and cleaning agents. The invention relates to the use of a dispersin or of a dispersin-containing agent to prevent and / or reduce bad odours on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washing or rinsing sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or conduits, or water inlets and / or drains, and / or to prevent and / or reduce and / or remove microbially induced deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washing or rinsing sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or conduits, or water inlets and / or drains, and / or to improve the hygiene of surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washing or rinsing sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or conduits, or water inlets and / or drains

Description

[0001] DETERGENT AND CLEANING AGENTS WITH DISPERSIN

[0002] DESCRIPTION

[0003] The invention lies in the field of washing and cleaning agents, in particular enzyme-containing washing and cleaning agents, and relates to the use of a dispersin or a dispersant-containing agent for preventing and / or reducing bad odors on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets, and / or for preventing and / or reducing and / or removing microbially caused deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets,and / or for improving the hygiene of surfaces, especially household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or conduits, water inlets and / or outlets. Furthermore, the invention relates to corresponding agents and methods.

[0004] The use of enzymes in detergents has been established in the state of the art for decades. They serve to expand the performance spectrum of the respective agents according to their special activities. These include, in particular, hydrolytic enzymes such as proteases, amylases, lipases, and cellulases. The first three hydrolyze proteins, starch, and fats and thus contribute directly to soil removal. Cellulases are used in textile detergents in particular because of their effect on fabrics. Another group of detergent enzymes are oxidative enzymes, in particular oxidases, which, if necessary in interaction with other components, preferably serve to bleach soils and / or generate the bleaching agents in situ. In addition to these enzymes, which are subject to continuous optimization, further enzymes are constantly being made available for use in detergents in order to optimally address special types of soiling, such as:Pectinases, pectate lysases, xanthanases, ß-glucanases, mannanases, dispersins, and the like, for the hydrolysis of specific plant polymers in particular. Generally, only selected enzymes are suitable for use in liquid surfactant-containing preparations. Many enzymes do not exhibit sufficient catalytic performance in such preparations. Therefore, for the use of enzymes in cleaning agents, high catalytic activity under conditions such as those encountered during a wash cycle and high storage stability are particularly desirable. Continuously changing demands are placed on the packaging and presentation of detergents and cleaning agents. For some time now, a key focus has been on convenient dosing of detergents and cleaning agents by the consumer and on simplifying the steps required to carry out a washing or cleaning process.One technical solution is pre-portioned detergents or cleaning agents, for example, foil pouches with one or more compartments for solid and / or liquid detergents and cleaning agents. A relevant trend for the production of these foil pouches is the miniaturization of these foil pouches. In addition to increased consumer acceptance due to simplified handling, this development is driven by sustainability aspects, for example, with regard to transport volumes and costs and the amount of packaging used. The concentration of modern detergents and cleaning agents requires the progressive optimization of existing formulations and the development of new, more effective formulations.One aim of these developments, which generally rely on known washing and cleaning active ingredients, is to achieve an increase in the washing or cleaning performance of the formulations while maintaining or reducing the dosage.

[0005] The most important criterion when cleaning textiles and / or hard surfaces is cleaning performance on a wide variety of soils. Even though the cleaning performance of the detergents and cleaning agents used today is generally high, the problem arises, partly due to the general trend toward the increased use of low-temperature programs and the trend toward compacting formulations (particularly in pre-portioned forms), that many of the conventional detergent and cleaning agent formulations exhibit inadequate cleaning performance, especially on stubborn soils. Such inadequate cleaning performance leads to consumer dissatisfaction and leads to the pretreatment of such soils by the consumer, which in turn increases water and energy consumption.

[0006] The term "malodor," as used herein, refers to an odor that is undesirable on surfaces, in particular household surfaces, dishes, textiles, and cleaned objects, especially cleaned textiles and cleaned dishes. The cleaned object should smell fresh and clean without any malodor. A wide variety of causes can cause an unpleasant odor, so-called malodor, on surfaces, in particular household surfaces, as well as on washed textiles and / or cleaned hard surfaces, especially dishes. The term "household surface," as used herein, refers, for example, to surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.Bad odors are a known problem, particularly with textiles and washing machines. But they can also be found in dishwashers. All components that come into contact with soiled objects and / or water, especially used water, and / or washing / cleaning solutions are affected, such as surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets. One, if not the most common, cause of this is organic in nature, such as sebum, body soils (e.g., body oils, dead skin cells, and sweat), cell residues, food residues, body excretions, biofilms, and extracellular polymeric substances (EPS; e.g., extracellular polymeric saccharides). Of particular note here are microbially caused biodeposits, i.e.,Microbially caused bad odor as a result of microbially caused bio-deposits. Another example of unpleasant odors is sweat or body odor that clings to an object that has come into contact with people or animals. Bad odors are found not only in the washing machine, but also on freshly washed, still damp laundry, as well as on dry laundry, and on numerous other surfaces, particularly household surfaces that come into contact with dirty objects and / or water, especially used water, and / or washing / cleaning solution, such as surfaces in washing machines or dishwashers, pump sumps, storage containers, items, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.Deposits, bio-deposits, body soils and the like not only lead to bad odors, but can also become visible as soiling on textiles and / or surfaces, in particular household surfaces such as surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, e.g. in the form of deposits, smears, encrustations, yellowing, underarm stains and greying. Due to their typically adhesive texture, bio-deposits and the like also allow other bad odor and / or dirt particles to adhere. However, bad odors can also be present before the microbially caused deposits lead to visible soiling.

[0007] The term "biofilm" as used herein refers not only to biofilms in the narrow sense, such as microbial deposits, biofilms, or the like, which can be formed by any microorganisms whose cells adhere to each other and / or to a surface, e.g., textiles, tableware, a hard surface, or any other type of surface, but also more generally to any biofilms, biosoils, biologically-induced deposits, or the like, including, but not limited to, degradation and deposition products of microorganisms that adhere to a surface, e.g., textiles, tableware, a hard surface, or any other type of surface. These adherent cells are often embedded in a self-produced matrix of extracellular polymeric substance (EPS). Biofilm EPS is a polymeric conglomerate generally consisting of extracellular DNA, proteins, and polysaccharides.Biofilms can form on living or non-living surfaces. The microbial cells that grow in a biofilm are physiologically different from the planktonic cells of the same organism, which, in contrast, are single cells that float or can swim in a liquid medium. Bacterial cells living in a biofilm usually have significantly different properties than planktonic bacterial cells of the same species because the dense and protected environment of the film allows them to cooperate and interact in different ways. One advantage of this environment for the microorganisms is increased resistance to detergents and antibiotics because the dense extracellular matrix and outer cell layer protect the interior of the community. Biofilm-producing bacteria on textiles include, for example, the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp., Staphylococcus epidermidis and Stenotrophomonas sp. Biofilm-forming bacteria can be found on hard surfaces, for example, among the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp, Staphylococcus epidermidis, Staphylococcus aureus and Stenotrophomonas sp.

[0008] EP 3892708, WO 2021 / 122117, WO 2021 / 122118, WO 2021 / 122120, WO 2021 / 122121, and WO 2022 / 008387 describe detergents and cleaning agents containing dispersin and enzymes. It has also been described that dispersin can degrade biofilm and EPS, for example.

[0009] Accumulated organic soiling is often difficult to remove, and commercially available detergents and cleaning products do not sufficiently reduce the unpleasant odor. Instead, the unpleasant odor is masked through the use of various fragrances, which consumers often perceive as a cleaning effect, even though the causes of the unpleasant odors are not actually eliminated. As a result, more and more organic material, and thus more and more unpleasant odors, accumulate over time. More and more fragrances have to be added to prevent the textiles from smelling worse and worse. However, many fragrances have side effects, such as allergenic properties, which can manifest themselves in the form of skin rashes. In addition, many of these substances are natural chemicals and are harmful to the environment if they enter wastewater.In recent years, fragrances have increasingly been used in the form of fragrance microcapsules, although it is well known that microcapsules are considered microplastics and therefore potentially harmful to the environment. Furthermore, fragrances are expensive and contribute to the overall cost of detergents and cleaning products.

[0010] There is therefore a need to further improve the cleaning performance of enzyme-containing washing and / or cleaning agents, in particular pre-portioned textile detergents, particularly with regard to stubborn soiling. Furthermore, there is a need to improve the bad odor in washing machines and / or dishwashers and / or on the washed textiles or cleaned surfaces, in particular dishes, as well as surfaces, in particular household surfaces, such as surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets. Furthermore, there is a need to improve hygiene in washing machines and / or dishwashers, as well as to reliably remove microbially caused bio-deposits from surfaces, in particular household surfaces, such asSurfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets. This also includes (semi-)automated washing or cleaning systems such as robot mops or wet vacuum cleaners.

[0011] Surprisingly, it has now been found that the dispersin according to the invention reliably removes microbially caused bad odors as well as microbially caused deposits, bio-coatings and the like from surfaces, in particular household surfaces, such as surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets.

[0012] The invention therefore relates, in a first aspect, to the use of a dispersin which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, based on the numbering according to SEQ ID NO:1, at least one of the positions which correspond to the positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135,

[0013] 138, 139, 140, 142, 145, 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175,

[0014] 177, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218,

[0015] 221 , 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 ,

[0016] 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, has at least one amino acid substitution and / or deletion, for preventing and / or reducing bad odors on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, Storage container, water reservoir, washing drum, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets, and / or for preventing and / or reducing and / or removing microbial deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage container, water reservoir, washing drum, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims,Siphons, water pipes and / or lines, water inlets and / or outlets, and / or to improve the hygiene of surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage tanks, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.

[0017] In a further aspect, the invention relates to the use of a dispersin which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to the positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105,

[0018] 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135,

[0019] 138, 139, 140, 142, 145, 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175,

[0020] 177, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218,

[0021] 221 , 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272, 273, 274, 276, 278, 279, 280, 281 , 282, 283, 284, 287, 288, 290, 291 , 296, 300, 301 , 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, at least one amino acid substitution and / or deletion selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71 H, L71 N, L71 R, L71V, L71W, S72*, S72C, S72D, S72E, S72F,S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81 L, D81 R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111 E, D111 M, D111 N, D111Q, D111 R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F,P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151 D, G151 L, G151 N, G151 P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171 E, D171 K, D171 L, D171 M, D171 Q, D171 R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181 D, S181 F, S181G, S181 N, S181 P, S181 Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E,A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251 L, S251 N, S251 R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261 D, E261 R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F,A271 D, A271G, H272D, H272I, Y281 P, Y282E, Y282N, Y281 R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281 C, Y281H, Y281 K, Y281 N, Y281 P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291 L, K291 R, K291V, T296C, E300A, E300D, H301 C, H301 N, H301 R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R and combinations thereof, for preventing and / or reducing bad odors on surfaces, in particular household surfaces,preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, and / or for preventing and / or reducing and / or removing microbially caused deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, and / or for improving the hygiene of surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, Siphons, water pipes and / or lines,Water inlets and / or outlets.,

[0022] In preferred embodiments, the dispersin is contained in a washing and / or cleaning agent, wherein the agent is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the agent, in a 1 wt. % solution in deionized water at 20°C, has a pH in a range from about 6 to about 11, in particular from about 6.5 to about 10.5, more preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

[0023] In further preferred embodiments, the dispersin or the dispersin-containing washing and / or cleaning agent is used in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

[0024] Further objects of the invention relate to an agent for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that the agent contains a dispersin according to the invention, and to a method for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines,Water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that in at least one process step a dispersin according to the invention or an agent according to the invention is used, preferably in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

[0025] These and other aspects, features, and advantages of the invention will become apparent to those skilled in the art from a study of the following detailed description and claims. Any feature of one aspect of the invention may be employed in any other aspect of the invention. Furthermore, it is to be understood that the examples contained herein are intended to describe and illustrate the invention, but not to limit it, and in particular, the invention is not limited to these examples.

[0026] All percentages are by weight (wt%) unless otherwise stated. Numerical ranges expressed in the format "from x to y" include the stated values. Where multiple preferred numerical ranges are specified in this format, it is understood that all ranges resulting from the combination of the various endpoints are also included.

[0027] "At least one" as used herein means one or more, i.e. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or more.

[0028] The term "washing and / or cleaning agent", "washing and cleaning agent" or "washing or cleaning agent", as used herein, is synonymous with the term "agent" and refers to a composition for cleaning textiles and / or hard surfaces, in particular dishes, as explained in the description.

[0029] "Approximately," "approximately," or "approximately," as used herein with reference to a numerical value, refers to the corresponding numerical value ±10%, preferably ±5%.

[0030] "Liquid," as used herein, includes liquids and gels, as well as pasty compositions. It is preferred that the liquid compositions be flowable and pourable at room temperature, but it is also possible that they have a yield point.

[0031] A substance, e.g. a composition or an agent, is solid according to the definition of the invention if it is in the solid state at 25°C and 1013 mbar.

[0032] A substance, e.g., a composition or agent, is liquid according to the invention's definition if it exists in the liquid state at 25°C and 1013 mbar. Liquid also includes gel.

[0033] "Variant," as used herein, refers to natural or artificially produced variations of a native enzyme that have an amino acid sequence modified from the reference form.

[0034] The term "deep cleaning" as used herein means the dissolution or removal of visible and invisible components of organic substances, e.g. sebum, cell residues, body dirt (body oil), sebum, biofilms, biofilms (including e.g. polysaccharides, PNAG (poly-N-acetyl-glucosamine)), body oils or other components contained in organic substances, not only at the level visible to the naked eye, but also the dissolution or removal of microbially caused deposits not visible to the naked eye, in particular deposits, biofilms and the like between individual fibers of textiles.

[0035] The term "textile," as used herein, refers to any textile material, including yarns, yarn precursors, fibers, nonwovens, natural materials, synthetic materials, and all other textile materials, fabrics made from these materials, and products made from fabrics (e.g., garments and other articles). The textile or fabric may be in the form of knits, wovens, denims, nonwovens, felts, yarns, and terry cloth. The textile may be cellulose-based, such as natural cellulose fibers like cotton, flax / linen, jute, ramie, sisal, or coconut fibers, or man-made cellulose fibers (e.g., from wood pulp) such as viscose / rayon, cellulose acetate fibers (Tricell), lyocell, or mixtures thereof. The textile or fabric may also be made of non-cellulose fibers, e.g.,Made from natural polyamides such as wool, camel, cashmere, mohair, rabbit, and silk, or from synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene, and spandex / elastane, or blends thereof, as well as blends of cellulosic and non-cellulosic fibers. Examples of blends include blends of cotton and / or rayon / viscose with one or more accompanying materials such as wool, synthetic fibers (e.g., polyamide fibers, acrylic fibers, polyester fibers, polyvinyl chloride fibers, polyurethane fibers, polyurea fibers, aramid fibers), and / or cellulosic fibers (e.g., rayon / viscose, ramie, flax / linen, jute, cellulose acetate fibers, lyocell). The fabric may be conventional washable laundry, e.g., soiled household linen. When the term "fabric" or "garment" is used, it is intended to include the broader term "textiles."

[0036] The present invention is based on the surprising discovery of the inventors that the dispersin according to the invention reliably removes microbially caused bad odors as well as microbially caused deposits, bio-coatings and the like from surfaces, in particular household surfaces, such as surfaces in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.

[0037] According to the invention, a dispersin which has ß-1,6-N-acetylglucosaminidase activity comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150,

[0038] 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189,

[0039] 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234,

[0040] 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272,

[0041] 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, has at least one amino acid substitution and / or deletion, for preventing and / or reducing bad odors on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or -pipes, water inlets and / or outlets, and / or for preventing and / or reducing and / or removing microbial deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines,Water inlets and / or outlets, and / or to improve the hygiene of surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.

[0042] This is particularly surprising since no such use of a dispersin for malodor reduction and / or biodegradation removal and / or improved hygiene has been described so far.

[0043] Dispersins belong to the group of hexosaminidases or ß-N-acetylglucosamine minidases (EC 3.2.1.52). These are polypeptides that catalyze the hydrolysis of N-acetyl-D-hexosamine or N-acetylglucosamine polymers. Dispersin B is a ß-hexosaminidase that specifically hydrolyzes ß-1,6-glycosidic bonds of acetylglucosamine polymers found, for example, in biofilm, EPS (extracellular polymer structures), cell debris, biofilms, and other so-called biosoils (i.e., soils of biological origin).

[0044] According to the invention, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity and comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, and based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113,

[0045] 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145,

[0046] 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185,

[0047] 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227,

[0048] 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272, 273, 274, 276, 278, 279, 280, 281 , 282, 283, 284, 287, 288, 290, 291 , 296, 300, 301 , 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, has at least one amino acid substitution and / or deletion.

[0049] In preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, and based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 200,203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272, 273, 274, 276, 278, 279, 280, 281 , 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, at least one amino acid substitution and / or deletion selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N,L71 R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81 L, D81 R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111 E, D111 M, D111 N, D111Q, D111 R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H,Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151 D, G151 L, G151 N, G151 P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171 E, D171 K, D171 L, D171 M, D171 Q, D171 R, D171V, D171W, D171Y, I173C, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181 D, S181 F, S181G, S181 N, S181 P, S181 Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L,E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251 L, S251 N, S251 R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261 D, E261 R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F,N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271 D, A271G, H272D, H272I, Y281 P, Y282E, Y282N, Y281 R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281 C, Y281 H, Y281 K, Y281 N, Y281 P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291 L, K291 R, K291V, T296C, E300A, E300D, H301 C, H301 N, H301 R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R and combinations thereof.

[0050] In further preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, and based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 22, 23, 25, 30, 49, 56, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 114, 123, 124, 125, 135, 138, 170, 171 , 173, 174, 175, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281 , 282, 283, 284, 288, 290, 291 , 296, 303, 304, 305, 306, 308, 309, 312, 314, 315,319 and 321, at least one amino acid substitution and / or deletion selected from the group consisting of D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171 E, D171 K, D171Y, D171 M, D171 Q, D171 L, I173C, D174W, D174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181 F, S181Q, S181G, S181 N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A,F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281 P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291 R, T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, N323R and combinations thereof.

[0051] In further preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, and based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 3, 15, 17, 49, 59, 140, 163, 186, 207, 215, 218, 225, 227, 232, 235, 237, 252, 260, 267, 272, 279, 281, 288, 308, 309 and 312, at least one amino acid substitution and / or deletion selected from the group consisting of Q3F, Q3I, H15Y, T17W, A49W, N59E, V140I, S163P, S186R, D207N, Q215K, T218Q, S225G, N227T, E232D, G235W, S237W, N252P, N260Q, N267T, H272V, H272P, S279D, Y281P, S288P, K308Q, K309E,K312Q and combinations thereof.,

[0052] In particularly preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, and based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, having at least one amino acid substitution and / or deletion selected from the group consisting of Q3I, H15Y, A49W, N59E, S163P, S186R, S225G, N227T, E232D, G235W, N252P, N260Q, H272V, S279D, Y281P, K308Q, K309E, K312Q and combinations thereof.

[0053] In preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, has at least one, and increasingly preferably two, three, four, five, six, seven, eight or nine amino acid substitutions, wherein the Dispersin has an amino acid substitution combination selected from the group consisting of Q3I-A49W, Q3I-N59E, Q3I-S163P, Q3I-S186R, Q3I-Q215K, Q3I-S225G, Q3I-N227T, Q3I-N252P, Q3I-N267T, Q3I-F276A, Q3I-Y281P, Q3I-K308Q, Q3I-K308E, Q3I-K309E, Q3I-K312Q, Q3I-K312E, Q3I-H15Y, Q3I-E232D, Q3I-G235W, Q3I-N260Q, Q3I-H272V, Q3I-S279D, Q3I-H15Y-A49W, Q3I-H15Y-N59E, Q3I-H15Y-S163P, Q3I-H15Y-S186R, Q3I-H15Y-S225G, Q3I-H15Y-N227T,Q3I-H15Y-E232D, Q3I-H15Y-G235W, Q3I-H15Y- N252P, Q3I-H15Y-N260Q, Q3I-H15Y-H272V, Q3I-H15Y-S279D, Q3I-H15Y-Y281 P, Q3I-H15Y- K308Q, Q3I-H15Y-K309E, Q3I-H15Y-K312Q, Q3I-A49W-N59E, Q3I-A49W-S163P, Q3I-A49W- S186R, Q3I-A49W-S225G, Q3I-A49W-N227T, Q3I-A49W-E232D, Q3I-A49W-G235W, Q3I-A49W- N252P, Q3I-A49W-N260Q, Q3I-A49W-H272V, Q3I-A49W-S279D, Q3I-A49W-Y281 P, Q3I-A49W- K308Q, Q3I-A49W-K309E, Q3I-A49W-K312Q, Q3I-N59E-S163P, Q3I-N59E-S186R, Q3I-N59E- S225G, Q3I-N59E-N227T, Q3I-N59E-E232D, Q3I-N59E-G235W, Q3I-N59E-N252P, Q3I-N59E- N260Q, Q3I-N59E-H272V, Q3I-N59E-S279D, Q3I-N59E-Y281 P, Q3I-N59E-K308Q, Q3I-N59E- K309E, Q3I-N59E-K312Q, Q3I-S163P-S186R, Q3I-S163P-S225G, Q3I-S163P-N227T, Q3I-S163P- E232D, Q3I-S163P-G235W, Q3I-S163P-N252P, Q3I-S163P-N260Q, Q3I-S163P-H272V, Q3I-S163P- S279D, Q3I-S163P-Y281 P, Q3I-S163P-K308Q, Q3I-S163P-K309E, Q3I-S163P-K312Q, Q3I-S186R- S225G, Q3I-S186R-N227T, Q3I-S186R-E232D, Q3I-S186R-G235W, Q3I-S186R-N252P, Q3I-S186R- N260Q, Q3I-S186R-H272V, Q3I-S186R-S279D,Q3I-S186R-Y281 P, Q3I-S186R-K308Q, Q3I-S186R- K309E, Q3I-S186R-K312Q, Q3I-S225G-N227T, Q3I-S225G-E232D, Q3I-S225G-G235W, Q3I-S225G- N252P, Q3I-S225G-N260Q, Q3I-S225G-H272V, Q3I-S225G-S279D, Q3I-S225G-Y281 P, Q3I-S225G- K308Q, Q3I-S225G-K309E, Q3I-S225G-K312Q, Q3I-N227T-E232D, Q3I-N227T-G235W, Q3I-N227T- N252P, Q3I-N227T-N260Q, Q3I-N227T-H272V, Q3I-N227T-S279D, Q3I-N227T-Y281 P, Q3I-N227T- K308Q, Q3I-N227T-K309E, Q3I-N227T-K312Q, Q3I-E232D-G235W, Q3I-E232D-N252P, Q3I-E232D- N260Q, Q3I-E232D-H272V, Q3I-E232D-S279D, Q3I-E232D-Y281 P, Q3I-E232D-K308Q, Q3I-E232D- K309E, Q3I-E232D-K312Q, Q3I-G235W-N252P, Q3I-G235W-N260Q, Q3I-G235W-H272V, Q3I- G235W-S279D, Q3I-G235W-Y281 P, Q3I-G235W-K308Q, Q3I-G235W-K309E, Q3I-G235W-K312Q, Q3I-N252P-N260Q, Q3I-N252P-H272V, Q3I-N252P-S279D, Q3I-N252P-Y281 P, Q3I-N252P-K308Q, Q3I-N252P-K309E, Q3I-N252P-K312Q, Q3I-N260Q-H272V, Q3I-N260Q-S279D, Q3I-N260Q-Y281 P, Q3I-N260Q-K308Q, Q3I-N260Q-K309E, Q3I-N260Q-K312Q, Q3I-H272V-S279D, Q3I-H272V-Y281 P,Q3I-H272V-K308Q, Q3I-H272V-K309E, Q3I-H272V-K312Q, Q3I-S279D-Y281 P, Q3I-S279D-K308Q, Q3I-S279D-K309E, Q3I-S279D-K312Q, Q3I-Y281 P-K308Q, Q3I-Y281 P-K309E, Q3I-Y281 P-K312Q, Q3I-K308Q-K309E, Q3I-K308Q-K312Q, Q3I-K309E-K312Q, Q3I-Q225G-N227T-N252P-N260Q- S186R-K308Q-K309E-K312Q, Q3I-S163P-Q225G-N227T-N252P-N260Q-K308Q-K309E-K312Q, Q3F-A49W, Q3F-N59E, Q3F-S163P, Q3F-S186R, Q3F-Q215K, Q3F-S225G, Q3F-N227T, Q3F- N252P, Q3F-N267T, Q3F-F276A, Q3F-Y281 P, Q3F-K308Q, Q3F-K308E, Q3F-K309E, Q3F-K312E, Q3F-K312Q, Q3F-A49W-Q215K-F276A-K308E-K309E-K312E-N227T, Q3F-A49W-Q215K-F276A- K308E-K309E-K312E-N267T, Q3F-A49W-Q215K-F276A-K308E-K309E-N227T-N267T, Q3F-A49W- Q215K-F276A-K308E-K312E-N227T-N267T, Q3F-A49W-Q215K-F276A-K309E-K312E-N227T- N267T, Q3F-A49W-Q215K-K308E-K309E-K312E-N227T-N267T, Q3F-A49W-F276A-K308E-K309E- K312E-N227T-N267T, Q3F-Q215K-F276A-K308E-K309E-K312E-N227T-N267T, Q3F-Q215K-N227T- N252P-N267T-F276A-K308E-K309E-K312E, Q3F-S163P-Q215K-N227T-N252P-N267T-K308E- K309E-K312E,Q3F-A49W-Q215K-F276A-K308E-K309E-K312E-N227T-N267T, H15Y-A49W, H15Y- N59E, H15Y-S163P, H15Y-S186R, H15Y-S225G, H15Y-N227T, H15Y-E232D, H15Y-235W, H15Y- N252P, H15Y-N260Q, H15Y-H272V, H15Y-S279D, H15Y-Y281 P, H15Y-K308Q, H15Y-K309E, H15Y- K312Q, H15Y-A49W-N59E, H15Y-A49W-S163P, H15Y-A49W-S186R, H15Y-A49W-S225G, H15Y- A49W-N227T, H15Y-A49W-E232D, H15Y-A49W-G235W, H15Y-A49W-N252P, H15Y-A49W-N260Q, H15Y-A49W-H272V, H15Y-A49W-S279D, H15Y-A49W-Y281 P, H15Y-A49W-K308Q, H15Y-A49W- K309E, H15Y-A49W-K312Q, H15Y-N59E-S163P, H15Y-N59E-S186R, H15Y-N59E-S225G, H15Y- N59E-N227T, H15Y-N59E-E232D, H15Y-N59E-G235W, H15Y-N59E-N252P, H15Y-N59E-N260Q, H15Y-N59E-H272V, H15Y-N59E-S279D, H15Y-N59E-Y281 P, H15Y-N59E-K308Q, H15Y-N59E- K309E, H15Y-N59E-K312Q, H15Y-S163P-S186R, H15Y-S163P-S225G, H15Y-S163P-N227T, H15Y- S163P-E232D, H15Y-S163P-G235W, H15Y-S163P-N252P, H15Y-S163P-N260Q, H15Y-S163P- H272V, H15Y-S163P-S279D, H15Y-S163P-Y281 P, H15Y-S163P-K308Q, H15Y-S163P-K309E, H15Y-S163P-K312Q, H15Y-S186R-S225G,H15Y-S186R-N227T, H15Y-S186R-E232D, H15Y- S186R-G235W, H15Y-S186R-N252P, H15Y-S186R-N260Q, H15Y-S186R-H272V, H15Y-S186R- S279D, H15Y-S186R-Y281 P, H15Y-S186R-K308Q, H15Y-S186R-K309E, H15Y-S186R-K312Q, H15Y-S225G-N227T, H15Y-S225G-E232D, H15Y-S225G-G235W, H15Y-S225G-N252P, H15Y- S225G-N260Q, H15Y-S225G-H272V, H15Y-S225G-S279D, H15Y-S225G-Y281 P, H15Y-S225G- K308Q, H15Y-S225G-K309E, H15Y-S225G-K312Q, H15Y-N227T-E232D, H15Y-N227T-G235W, H15Y-N227T-N252P, H15Y-N227T-N260Q, H15Y-N227T-H272V, H15Y-N227T-S279D, H15Y- N227T-Y281 P, H15Y-N227T-K308Q, H15Y-N227T-K309E, H15Y-N227T-K312Q, H15Y-E232D- G235W, H15Y-E232D-N252P, H15Y-E232D-N260Q, H15Y-E232D-H272V, H15Y-E232D-S279D, H15Y-E232D-Y281 P, H15Y-E232D-K308Q, H15Y-E232D-K309E, H15Y-E232D-K312Q, H15Y- G235W-N252P, H15Y-G235W-N260Q, H15Y-G235W-H272V, H15Y-G235W-S279D, H15Y-G235W- Y281 P, H15Y-G235W-K308Q, H15Y-G235W-K309E, H15Y-G235W-K312Q, H15Y-N252P-N260Q, H15Y-N252P-H272V, H15Y-N252P-S279D, H15Y-N252P-Y281 P, H15Y-N252P-K308Q,H15Y- N252P-K309E, H15Y-N252P-K312Q, H15Y-N260Q-H272V, H15Y-N260Q-S279D, H15Y-N260Q- Y281 P, H15Y-N260Q-K308Q, H15Y-N260Q-K309E, H15Y-N260Q-K312Q, H15Y-H272V-S279D, H15Y-H272V-Y281 P, H15Y-H272V-K308Q, H15Y-H272V-K309E, H15Y-H272V-K312Q, H15Y- S279D-Y281 P, H15Y-S279D-K308Q, H15Y-S279D-K309E, H15Y-S279D-K312Q, H15Y-Y281 P- K308Q, H15Y-Y281 P-K309E, H15Y-Y281 P-K312Q, H15Y-K308Q-K309E, H15Y-K308Q-K312Q, H15Y-K309E-K312Q, A49W-N59E, A49W-S163P, A49W-S186R, A49W-Q215K, A49W-S225G, A49W-N227T, A49W-N252P, A49W-N267T, A49W-F276A, A49W-Y281 P, A49W-K308Q, A49W- K308E, A49W-K309E, A49W-K312Q, A49W-K312E, A49W-E232D, A49W-G235W, A49W-N260Q, A49W-H272V, A49W-S279D, A49W-N59E-S163P, A49W-N59E-S186R, A49W-N59E-S225G, A49W- N59E-N227T, A49W-N59E-E232D, A49W-N59E-G235W, A49W-N59E-N252P, A49W-N59E-N260Q, A49W-N59E-H272V, A49W-N59E-S279D, A49W-N59E-Y281 P, A49W-N59E-K308Q, A49W-N59E- K309E, A49W-N59E-K312Q, A49W-S163P-S186R, A49W-S163P-S225G, A49W-S163P-N227T, A49W-S163P-E232D, A49W-S163P-G235W,A49W-S163P-N252P, A49W-S163P-N260Q, A49W- S163P-H272V, A49W-S163P-S279D, A49W-S163P-Y281 P, A49W-S163P-K308Q, A49W-S163P- K309E, A49W-S163P-K312Q, A49W-S186R-S225G, A49W-S186R-N227T, A49W-S186R-E232D, A49W-S186R-G235W, A49W-S186R-N252P, A49W-S186R-N260Q, A49W-S186R-H272V, A49W- S186R-S279D, A49W-S186R-Y281 P, A49W-S186R-K308Q, A49W-S186R-K309E, A49W-S186R- K312Q, A49W-S225G-N227T, A49W-S225G-E232D, A49W-S225G-G235W, A49W-S225G-N252P, A49W-S225G-N260Q, A49W-S225G-H272V, A49W-S225G-S279D, A49W-S225G-Y281 P, A49W- S225G-K308Q, A49W-S225G-K309E, A49W-S225G-K312Q, A49W-N227T-E232D, A49W-N227T- G235W, A49W-N227T-N252P, A49W-N227T-N260Q, A49W-N227T-H272V, A49W-N227T-S279D, A49W-N227T-Y281 P, A49W-N227T-K308Q, A49W-N227T-K309E, A49W-N227T-K312Q, A49W- E232D-G235W, A49W-E232D-N252P, A49W-E232D-N260Q, A49W-E232D-H272V, A49W-E232D- S279D, A49W-E232D-Y281 P, A49W-E232D-K308Q, A49W-E232D-K309E, A49W-E232D-K312Q, A49W-G235W-N252P, A49W-G235W-N260Q, A49W-G235W-H272V, A49W-G235W-S279D,A49W- G235W-Y281 P, A49W-G235W-K308Q, A49W-G235W-K309E, A49W-G235W-K312Q, A49W-N252P- N260Q, A49W-N252P-H272V, A49W-N252P-S279D, A49W-N252P-Y281 P, A49W-N252P-K308Q, A49W-N252P-K309E, A49W-N252P-K312Q, A49W-N260Q-H272V, A49W-N260Q-S279D, A49W- N260Q-Y281 P, A49W-N260Q-K308Q, A49W-N260Q-K309E, A49W-N260Q-K312Q, A49W-H272V- S279D, A49W-H272V-Y281 P, A49W-H272V-K308Q, A49W-H272V-K309E, A49W-H272V-K312Q, A49W-S279D-Y281 P, A49W-S279D-K308Q, A49W-S279D-K309E, A49W-S279D-K312Q, A49W- Y281 P-K308Q, A49W-Y281 P-K309E, A49W-Y281 P-K312Q, A49W-K308Q-K309E, A49W-K308Q- K312Q, A49W-K309E-K312Q, A49W-N227T-F276A-K308E, A49W-S186R-N227T-F276A, A49W- S186R-N227T-K308E, A49W-N227T-A269C-Y282C-K308E, A49W-S186R-N227T-F276A-K308E, A49W-Y124R-N227T-F276A-K308E, A49W-Y124R-S186R-N227T-F276A, A49W-Y124R-S186R- N227T-K308E, A49W-A269C-F276A-Y282C-K308E, A49W-N227T-A269C-F276A-Y282C, A49W- F276A-K308E, A49W-N227T-F276A, A49W-Y124R-S186R-N227T-F276A-K308E, A49W-N227T- A269C-F276A-Y282C-K308E,A49W-S186R-N227T-A269C-F276A-Y282C, A49W-S186R-N227T- A269C-Y282C-K308E, A49W-S186R-N227T-A269C-F276A-Y282C-K308E, A49W-Y124R-N227T- A269C-F276A-Y282C-K308E, A49W-Y124R-S186R-N227T-A269C-F276A-Y282C, A49W-Y124R- S186R-N227T-A269C-Y282C-K308E, A49W-Y124R-S186R-N227T-A269C-F276A-Y282C-K308E, A49W-S163P-Q215K-N227T-N252P-N267T-F276A-K309E-K312E, A49W-Q215K-N227T-N252P- N267T-F276A-K308E-K309E-K312E, A49W-S163P-Q225G-N227T-N252P-N260Q-S186R-K309E- K312Q, A49W-Q225G-N227T-N252P-N260Q-S186R-K308Q-K309E-K312Q, N59E-S163P, N59E- S186R, N59E-Q215K, N59E-S225G, N59E-N227T, N59E-N252P, N59E-N267T, N59E-F276A, N59E- Y281 P, N59E-K308Q, N59E-K308E, N59E-K309E, N59E-K312Q, N59E-K312E, N59E-E232D, N59E- G235W, N59E-N260Q, N59E-H272V, N59E-S279D, N59E-S163P-S186R, N59E-S163P-S225G, N59E-S163P-N227T, N59E-S163P-E232D, N59E-S163P-G235W, N59E-S163P-N252P, N59E- S163P-N260Q, N59E-S163P-H272V, N59E-S163P-S279D, N59E-S163P-Y281 P, N59E-S163P- K308Q, N59E-S163P-K309E, N59E-S163P-K312Q, N59E-S186R-S225G,N59E-S186R-N227T, N59E-S186R-E232D, N59E-S186R-G235W, N59E-S186R-N252P, N59E-S186R-N260Q, N59E- S186R-H272V, N59E-S186R-S279D, N59E-S186R-Y281 P, N59E-S186R-K308Q, N59E-S186R- K309E, N59E-S186R-K312Q, N59E-S225G-N227T, N59E-S225G-E232D, N59E-S225G-G235W, N59E-S225G-N252P, N59E-S225G-N260Q, N59E-S225G-H272V, N59E-S225G-S279D, N59E- S225G-Y281 P, N59E-S225G-K308Q, N59E-S225G-K309E, N59E-S225G-K312Q, N59E-N227T- E232D, N59E-N227T-G235W, N59E-N227T-N252P, N59E-N227T-N260Q, N59E-N227T-H272V, N59E-N227T-S279D, N59E-N227T-Y281 P, N59E-N227T-K308Q, N59E-N227T-K309E, N59E-N227T- K312Q, N59E-E232D-G235W, N59E-E232D-N252P, N59E-E232D-N260Q, N59E-E232D-H272V, N59E-E232D-S279D, N59E-E232D-Y281 P, N59E-E232D-K308Q, N59E-E232D-K309E, N59E- E232D-K312Q, N59E-G235W-N252P, N59E-G235W-N260Q, N59E-G235W-H272V, N59E-G235W- S279D, N59E-G235W-Y281 P, N59E-G235W-K308Q, N59E-G235W-K309E, N59E-G235W-K312Q, N59E-N252P-N260Q, N59E-N252P-H272V, N59E-N252P-S279D, N59E-N252P-Y281 P, N59E- N252P-K308Q,N59E-N252P-K309E, N59E-N252P-K312Q, N59E-N260Q-H272V, N59E-N260Q- S279D, N59E-N260Q-Y281 P, N59E-N260Q-K308Q, N59E-N260Q-K309E, N59E-N260Q-K312Q, N59E-H272V-S279D, N59E-H272V-Y281 P, N59E-H272V-K308Q, N59E-H272V-K309E, N59E- H272V-K312Q, N59E-S279D-Y281 P, N59E-S279D-K308Q, N59E-S279D-K309E, N59E-S279D- K312Q, N59E-Y281 P-K308Q, N59E-Y281 P-K309E, N59E-Y281 P-K312Q, N59E-K308Q-K309E, N59E-K308Q-K312Q, N59E-K309E-K312Q, N59E-N227T-N252P-E232D-S225G, N59E-N227T- N252P-E232D-K308Q, N59E-N227T-N252P-E232D-K309E, N59E-N227T-N252P-S225G-K308Q, N59E-N227T-N252P-S225G-K309E, N59E-N227T-N252P-K308Q-K309E, N59E-N227T-E232D- S225G-K308Q, N59E-N227T-E232D-S225G-K309E, N59E-N227T-E232D-K308Q-K309E, N59E- N227T-S225G-K308Q-K309E, N59E-N252P-E232D-S225G-K308Q, N59E-N252P-E232D-S225G- K309E, N59E-N252P-E232D-K308Q-K309E, N59E-N252P-S225G-K308Q-K309E, N59E-E232D- S225G-K308Q-K309E, N59E-N227T-S225G-K308Q-K312Q, N59E-N227T-S225G-K309E-K312Q, N59E-N227T-K308Q-K309E-K312Q, 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S186R-N227T-A269C-F276A-Y282C,S186R-N227T-A269C-Y282C-K308E, S186R-N227T-A269C-F276A-Y282C-K308E, S186R-N227T- N252P-S186R-K308Q-K309E-K312Q, S186R-N227T-S225G-S186R-K308Q-K309E-K312Q, S163P- N227T-N252P-S186R-K308Q-K309E-K312Q, Q215K-S225G, Q215K-N227T, Q215K-N252P, Q215K- N267T, Q215K-F276A, Q215K-Y281 P, Q215K-K308E, Q215K-K308Q, Q215K-K309E, Q215K- K312Q, Q215K-K312E, Q215K-F276A-K308E, Q215K-F276A-K312E, Q215K-K308E-K312E, Q215K- K309E-K312E, Q215K-N227T-F276A, Q215K-N227T-K308E, Q215K-N227T-K309E, Q215K-N227T- K312E, Q215K-N227T-N252P, Q215K-N227T-N267T, Q215K-N252P-F276A, Q215K-N252P-K309E, Q215K-N252P-K312E, Q215K-N252P-N267T, Q215K-N267T-F276A, Q215K-N267T-K312E, Q215K- N227T-N252P-N267T-F276A, Q215K-N227T-N252P-N267T-K308E, Q215K-N227T-N252P-N267T- K309E, Q215K-N227T-N252P-F276A-K308E, Q215K-N227T-N252P-F276A-K309E, Q215K-N227T- N252P-K308E-K309E, Q215K-N227T-N267T-F276A-K308E, Q215K-N227T-N267T-F276A-K309E, Q215K-N227T-N267T-K308E-K309E, Q215K-N227T-F276A-K308E-K309E, Q215K-N252P-N267T- F276A-K308E,Q215K-N252P-N267T-F276A-K309E, Q215K-N252P-N267T-K308E-K309E, Q215K- N252P-F276A-K308E-K309E, Q215K-N267T-F276A-K308E-K309E, Q215K-N227T-F276A-K308E- K312E, Q215K-N227T-F276A-K309E-K312E, Q215K-N227T-K308E-K309E-K312E, Q215K-F276A- K308E-K309E-K312E, Q215K-N227T-F276A-K308E-K309E-K312E, Q215K-N227T-N252P-F276A- K308E-K309E-K312E, Q215K-N227T-N267T-F276A-K308E-K309E-K312E, Q215K-N227T-N252P- N267T-F276A-K308E-K309E-K312E, S225G-N227T, S225G-N252P, S225G-N267T, S225G-F276A, S225G-Y281 P, S225G-K308E, S225G-K308Q, S225G-K309E, S225G-K312Q, S225G-K312E, S225G-E232D, S225G-G235W, S225G-N260Q, S225G-H272V, S225G-S279D, S225G-N227T- E232D, S225G-N227T-G235W, S225G-N227T-N252P, S225G-N227T-N260Q, S225G-N227T- H272V, S225G-N227T-S279D, S225G-N227T-Y281 P, S225G-N227T-K308Q, S225G-N227T-K309E, S225G-N227T-K312Q, S225G-E232D-G235W, S225G-E232D-N252P, S225G-E232D-N260Q, S225G-E232D-H272V, S225G-E232D-S279D, S225G-E232D-Y281 P, S225G-E232D-K308Q, S225G- E232D-K309E, S225G-E232D-K312Q,S225G-G235W-N252P, S225G-G235W-N260Q, S225G- G235W-H272V, S225G-G235W-S279D, S225G-G235W-Y281 P, S225G-G235W-K308Q, S225G- G235W-K309E, S225G-G235W-K312Q, S225G-N252P-N260Q, S225G-N252P-H272V, S225G- N252P-S279D, S225G-N252P-Y281 P, S225G-N252P-K308Q, S225G-N252P-K309E, S225G-N252P- K312Q, S225G-N260Q-H272V, S225G-N260Q-S279D, S225G-N260Q-Y281 P, S225G-N260Q- K308Q, S225G-N260Q-K309E, S225G-N260Q-K312Q, S225G-H272V-S279D, S225G-H272V- Y281 P, S225G-H272V-K308Q, S225G-H272V-K309E, S225G-H272V-K312Q, S225G-S279D-Y281 P, S225G-S279D-K308Q, S225G-S279D-K309E, S225G-S279D-K312Q, S225G-Y281 P-K308Q, S225G- Y281 P-K309E, S225G-Y281 P-K312Q, S225G-K308Q-K309E, S225G-K308Q-K312Q, S225G-K309E- K312Q, S225G-K308Q-K309E-K312Q, S225G-N227T-N252P-S186R-N260Q-K308E-K309E-K312E, N227T-N252P, N227T-N267T, N227T-F276A, N227T-Y281 P, N227T-K308Q, N227T-K308E, N227T- K309E, N227T-K312Q, N227T-K312E, N227T-E232D, N227T-G235W, N227T-N260Q, N227T- H272V, N227T-S279D, N227T-E232D-G235W, N227T-E232D-N252P,N227T-E232D-N260Q, N227T-E232D-H272V, N227T-E232D-S279D, N227T-E232D-Y281 P, N227T-E232D-K308Q, N227T- E232D-K309E, N227T-E232D-K312Q, N227T-G235W-N252P, N227T-G235W-N260Q, N227T- G235W-H272V, N227T-G235W-S279D, N227T-G235W-Y281 P, N227T-G235W-K308Q, N227T- G235W-K309E, N227T-G235W-K312Q, N227T-N252P-N260Q, N227T-N252P-H272V, N227T- N252P-S279D, N227T-N252P-Y281 P, N227T-N252P-K308Q, N227T-N252P-K309E, N227T-N252P- K312Q, N227T-N260Q-H272V, N227T-N260Q-S279D, N227T-N260Q-Y281 P, N227T-N260Q-K308Q, N227T-N260Q-K309E, N227T-N260Q-K312Q, N227T-H272V-S279D, N227T-H272V-Y281 P, N227T- H272V-K308Q, N227T-H272V-K309E, N227T-H272V-K312Q, N227T-S279D-Y281 P, N227T-S279D- K308Q, N227T-S279D-K309E, N227T-S279D-K312Q, N227T-Y281 P-K308Q, N227T-Y281 P-K309E, N227T-Y281 P-K312Q, N227T-K308Q-K309E, N227T-K308Q-K312Q, N227T-K309E-K312Q, N227T- N252P-K308E, N227T-N252P-K312E, N227T-N252P-N267T, N227T-N267T-F276A, N227T-N267T- K308E, N227T-N267T-K309E, N227T-N267T-K312E, N227T-F276A-K308E,N227T-F276A-K309E, N227T-F276A-K312E, N227T-K308E-K309E, N227T-K308E-K312E, N227T-K309E-K312E, N227T- F276A-K308E-K309E, N227T-F276A-K308E-K312E, N227T-F276A-K309E-K312E, N227T-K308E- K309E-K312E, N227T-S225G-K308Q-K309E, N227T-S225G-K308Q-K312Q, N227T-S225G-K309E- K312Q, N227T-K308Q-K309E-K312Q, N227T-N267T-F276A-K308E, N227T-N267T-F276A-K309E, N227T-N267T-F276A-K312E, N227T-N267T-S225G-K308Q, N227T-N267T-S225G-K309E, N227T- N267T-S225G-K312Q, N227T-N252P-N267T-F276A-K308E, N227T-N252P-N267T-F276A-K309E, N227T-N252P-N267T-K308E-K309E, N227T-N252P-F276A-K308E-K309E, N227T-N267T-F276A- K308E-K309E, N227T-F276A-K308E-K309E-K312E, N227T-N252P-E232D-S225G-K308Q, N227T- N252P-E232D-S225G-K309E, N227T-N252P-E232D-K308Q-K309E, N227T-N252P-S225G-K308Q- K309E, N227T-E232D-S225G-K308Q-K309E, N227T-S225G-K308Q-K309E-K312Q, N227T-N267T- F276A-K308E-K312E, N227T-N267T-F276A-K309E-K312E, N227T-E232D-S225G-K308Q-K312Q, N227T-E232D-S225G-K309E-K312Q, N227T-N267T-F276A-K308E-K309E-K312E,N227T-N252P- F276A-K308E-K309E-K312E, N227T-S225G-S186R-K308Q-K309E-K312Q, N227T-N252P-S186R- K308Q-K309E-K312Q, N227T-N252P-N267T-F276A-K308E-K309E-K312E, N227T-N252P-S225G- S186R-K308Q-K309E-K312Q, E232D-G235W, E232D-N252P, E232D-N260Q, E232D-H272V, E232D-S279D, E232D-Y281 P, E232D-K308Q, E232D-K309E, E232D-K312Q, E232D-G235W- N252P, E232D-G235W-N260Q, E232D-G235W-H272V, E232D-G235W-S279D, E232D-G235W- Y281 P, E232D-G235W-K308Q, E232D-G235W-K309E, E232D-G235W-K312Q, E232D-N252P- N260Q, E232D-N252P-H272V, E232D-N252P-S279D, E232D-N252P-Y281 P, E232D-N252P-K308Q, E232D-N252P-K309E, E232D-N252P-K312Q, E232D-N260Q-H272V, E232D-N260Q-S279D, E232D- N260Q-Y281 P, E232D-N260Q-K308Q, E232D-N260Q-K309E, E232D-N260Q-K312Q, E232D- H272V-S279D, E232D-H272V-Y281 P, E232D-H272V-K308Q, E232D-H272V-K309E, E232D-H272V- K312Q, E232D-S279D-Y281 P, E232D-S279D-K308Q, E232D-S279D-K309E, E232D-S279D-K312Q, E232D-Y281 P-K308Q, E232D-Y281 P-K309E, E232D-Y281 P-K312Q, E232D-K308Q-K309E, E232D- K308Q-K312Q,E232D-K309E-K312Q, G235W-N252P, G235W-N260Q, G235W-H272V, G235W- S279D, G235W-Y281 P, G235W-K308Q, G235W-K309E, G235W-K312Q, G235W-N252P-N260Q, G235W-N252P-H272V, G235W-N252P-S279D, G235W-N252P-Y281 P, G235W-N252P-K308Q, G235W-N252P-K309E, G235W-N252P-K312Q, G235W-N260Q-H272V, G235W-N260Q-S279D, G235W-N260Q-Y281 P, G235W-N260Q-K308Q, G235W-N260Q-K309E, G235W-N260Q-K312Q, G235W-H272V-S279D, G235W-H272V-Y281 P, G235W-H272V-K308Q, G235W-H272V-K309E, G235W-H272V-K312Q, G235W-S279D-Y281 P, G235W-S279D-K308Q, G235W-S279D-K309E, G235W-S279D-K312Q, G235W-Y281 P-K308Q, G235W-Y281 P-K309E, G235W-Y281 P-K312Q, G235W-K308Q-K309E, G235W-K308Q-K312Q, G235W-K309E-K312Q, G235W-N227T-S186R- K308Q-K309E-K312Q, G235W-N227T-N252P-S186R-N260Q-K308Q-K309E-K312Q, N252P-N267T, N252P-F276A, N252P-Y281 P, N252P-K308Q, N252P-K308E, N252P-K309E, N252P-K312Q, N252P- K312E, N252P-N260Q, N252P-H272V, N252P-S279D, N252P-N260Q-H272V, N252P-N260Q- S279D, N252P-N260Q-Y281 P, N252P-N260Q-K308Q, N252P-N260Q-K309E,N252P-N260Q- K312Q, N252P-H272V-S279D, N252P-H272V-Y281 P, N252P-H272V-K308Q, N252P-H272V-K309E, N252P-H272V-K312Q, N252P-S279D-Y281 P, N252P-S279D-K308Q, N252P-S279D-K309E, N252P- S279D-K312Q, N252P-Y281 P-K308Q, N252P-Y281 P-K309E, N252P-Y281 P-K312Q, N252P-K308Q- K309E, N252P-K308Q-K312Q, N252P-K309E-K312Q, N252P-F276A-K308E, N252P-F276A-K309E, N252P-F276A-K312E, N252P-K308E-K309E, N252P-K308E-K312E, N252P-N267T-F276A, N252P- N267T-K308E, N252P-N267T-K309E, N252P-N267T-K312E, N252P-N267T-F276A-K308E-K309E, N252P-E232D-S225G-K308Q-K309E, N260Q-H272V, N260Q-S279D, N260Q-Y281 P, N260Q- K308Q, N260Q-K309E, N260Q-K312Q, N260Q-H272V-S279D, N260Q-H272V-Y281 P, N260Q- H272V-K308Q, N260Q-H272V-K309E, N260Q-H272V-K312Q, N260Q-S279D-Y281 P, N260Q- S279D-K308Q, N260Q-S279D-K309E, N260Q-S279D-K312Q, N260Q-Y281 P-K308Q, N260Q- Y281 P-K309E, N260Q-Y281 P-K312Q, N260Q-K308Q-K309E, N260Q-K308Q-K312Q, N260Q- K309E-K312Q, N267T-F276A, N267T-Y281 P, N267T-K308E, N267T-K309E, N267T-K312Q, N267T- K312E,N267T-F276A-K308E, N267T-F276A-K309E, N267T-F276A-K312E, N267T-K308E-K309E, N267T-K308E-K312E, N267T-K309E-K312E, H272V-S279D, H272V-Y281 P, H272V-K308Q, H272V- K309E, H272V-K312Q, H272V-S279D-Y281 P, H272V-S279D-K308Q, H272V-S279D-K309E, H272V- S279D-K312Q, H272V-Y281 P-K308Q, H272V-Y281 P-K309E, H272V-Y281 P-K312Q, H272V-K308Q- K309E, H272V-K308Q-K312Q, H272V-K309E-K312Q, F276A-Y281 P, F276A-K308Q, F276A-K308E, F276A-K309E, F276A-K312Q, F276A-K312E, F276A-K308E-K309E, F276A-K308E-K312E, F276A- K309E-K312E, F276A-K308E-K309E-K312E, S279D-Y281 P, S279D-K308Q, S279D-K309E, S279D- K312Q, S279D-Y281 P-K308Q, S279D-Y281 P-K309E, S279D-Y281 P-K312Q, S279D-K308Q-K309E, S279D-K308Q-K312Q, S279D-K309E-K312Q, Y281 P-K308Q, Y281 P-K308E, Y281 P-K309E, Y281 P- K312Q, Y281 P-K312E, Y281 P-K308Q-K309E, Y281 P-K308Q-K312Q, Y281 P-K309E-K312Q, K308E- K312Q, K308E-K312E, K308Q-K312Q, K309E-K312Q, K309E-K312E, K308E-K309E, K308Q-K309E, K308Q-K309E-K312Q, K308E-K309E-K312E, K312E-N227T-F276A,K312E-N227T-K308E, K312E-N227T-K309E, K312E-N227T-K312E, K312E-F276A-K308E, K312E-F276A-K309E, K312E-F276A-K312E, K312E-K308E-K309E, K312E-K308E-K312E, K312E-K309E-K312E, K312E-N227T-F276A-K308E, K312E-N227T-F276A-K309E, K312E-N227T-F276A-K312E, K312E-N227T-K308E-K309E, K312E-N227T-K308E-K312E, K312E-N227T-K309E-K312E, K312E-F276A-K308E-K309E, K312E- F276A-K308E-K312E, K312E-F276A-K309E-K312E, K312E-K308E-K309E-K312E, K312Q-N227T-S225G-K308Q, K312Q-N227T-S225G-K309E, K312Q-N227T-S225G-K312Q, K312Q-N227T-K308Q-K309E, K312Q-N227T-K308Q-K312Q, K312Q-N227T-K309E-K312Q, K312Q-S225G-K308Q-K309E, K312Q-S225G-K308Q-K312Q, K312Q-S225G-K309E-K312Q and K312Q-K308Q-K309E-K312Q, based on the numbering according to SEQ ID NO: 1. In further preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence,which is at least 70%, and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96% or 96.5% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, has at least ten, and increasingly preferably eleven, twelve, 13, 14, 15, 16, 17, 18 or more amino acid substitutions, wherein the dispersin has an amino acid substitution combination which consists of the one from Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A-Y281 P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-F276A-S279G-K308E- K309E-K312E, Q3F-T17W-A49W-N59E-S163P-S186R-S225G-N227T-G235W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-F276A- S279G-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-K308E-K309E-K312E, Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T- F276A-S279G-K308E-K309E-K312E,Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T- F276A-S279G-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-F276A- S279G-Y281 P-K308E-K309E-K312E, Q3F-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G- N227T-G235W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R- S225G-N227T-N252P-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P- S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E, Q3F-H15Y- V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D- Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P- N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P- S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-K308Q-K309E-K312Q, Q3F-H15Y- T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-S237W-N252P-H272P- Y281 P-K308Q-K309E-K312Q, Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T-H272V- F276A-S279G-K308E-K309E-K312E,Q3F-S163P-S186R-Q215K-N227T-E232D-N252P-N267T- F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-S163P-Q215K-N227T-E232D-N252P-N267T- F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q- S225G-N227T-G235W-S237W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-S163P-Q215K- S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-K308E-K309E-K312E, Q3F-A49W- N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-F276A-S279G-Y281 P-K308E-K309E- K312E, Q3F-S163P-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E- K309E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G- Y281 P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-N252P-H272V-F276A- S279G-Y281 P-K308E-K309E-K312E, Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P- N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-S163P-S186R-Q215K-N227T- E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E,Q3F-S163P-S186R- Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F- T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G- Y281 P-K308E-K309E-K312Q, Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T- H272V-F276A-S279G-Y281 P-K308E-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G- N227T-E232D-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-S163P-S186R- Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T- H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-S163P-Q215K-S225G-N227T-E232D-N252P- N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-H15Y-S163P-S186R-Q215K- S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-F276A- S279D-Y281 P-K308E-K309E-K312Q,Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T- E232D-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-S163P- S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E- K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V- F276A-S279G-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G- N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y- A49W-N59E-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T- H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-A49W-N59E-S163P-S186R-S225G-N227T-Y281 P- K308Q-K309E-K312Q, Q3I-A49W-N59E-S163P-S186R-S225G-E232D-Y281 P-K308Q-K309E- K312Q, Q3I-A49W-N59E-S163P-S186R-S225G-G235W-Y281 P-K308Q-K309E-K312Q, Q3I-A49W- N59E-S163P-S186R-S225G-N252P-Y281 P-K308Q-K309E-K312Q, Q3I-A49W-N59E-S163P-S186R- S225G-N260Q-Y281 P-K308Q-K309E-K312Q, Q3I-A49W-N59E-S163P-S186R-S225G-N227T- H272V-Y281 P-K308Q-K309E,Q3I-A49W-N59E-S163P-S186R-S225G-N227T-S279D-Y281 P-K308Q- K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V- S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D- N252P-N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P- S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312E, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D- Y281 P-K308E-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R- S225G-N227T-E232D-Y281 P-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T- G235W-K308Q-K309E-K312Q, H15Y-A49W-S163P-S186R-S225G-N227T-Y281 P-S288P-K308Q- K309E-K312Q, H 15Y-A49W-S163P-S186R-S225G-N227T-N252P-Y281 P-K308Q-K309E-K312Q, H15Y-A49W-S163P-S186R-S225G-N227T-H272P-Y281 P-K308Q-K309E-K312Q, H15Y-A49W- S163P-S186R-S225G-N227T-S279D-Y281 P-K308Q-K309E-K312Q,H15Y-A49W-N59E-S163P- S186R-S225G-N227T-E232D-Y281 P-K308Q-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G- N227T-E232D-G235W-N252P-N260Q-H272P-S279D-Y281 P-K308Q-K309E-K312Q, H15Y-A49W- N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q- K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272V- Y281 P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272V-S279D-Y281 P-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G- N227T-E232D-N252P-N260Q-H272P-S279D-Y281 P-S288P-K308Q-K309E-K312Q, H15Y-A49W- N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272P-S279D-Y281 P-K308Q-K309E- K312Q, H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q- H272V-S279D-Y281 P-K308Q-K309E-K312Q, H15Y-T17W-A49W-N59E-S163P-S186R-S225G- N227T-E232D-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q, A49W-N59E-S163P- S186R-S225G-N227T-E232D-N252P-F276A-S279G-K308E-K309E-K312E,A49W-N59E-S163P- S186R-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E, A49W-N59E-S163P- S186R-N227T-E232D-N252P-F276A-S279G-Y281 P-K308E-K309E-K312E, A49W-N59E-S1 ßSP- SI 86R-S225G-N227T-N252P-F276A-S279G-Y281 P-K308E-K309E-K312E, A49W-N59E-S163P- S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-K308E-K309E-K312E, A49W-N59E- S163P-S186R-S225G-N227T-E232D-N252P-F276A-S279G-Y281 P-K308E-K309E-K312E, A49W- N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, A49W-N59E-S163P-S186R-S225G-N227T-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E- K312E, A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281 P- K308E-K309E-K312E, S163P-S186R-Q215K-N227T-E232D-N252P-N267T-F276A-S279G-Y281 P- K308E-K309E-K312E, S163P-S186R-S225G-N227T-E232D-N252P-N267T-F276A-S279G-Y281 P- K308E-K309E-K312E, S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-F276A-S279G- Y281 P-K308E-K309E-K312E, S163P-Q215K-S225G-N227T-E232D-N252P-N267T-F276A-S279G- Y281 P-K308E-K309E-K312E,S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V- F276A-S279G-K308E-K309E-K312E, S163P-Q215K-S225G-N227T-E232D-N252P-N267T-H272V- F276A-S279G-K308E-K309E-K312E, S163P-Q215K-N227T-E232D-N252P-N267T-H272V-F276A- S279G-Y281 P-K308E-K309E-K312E, S163P-S186R-Q215K-S225G-N227T-N252P-N267T-H272V- F276A-S279G-Y281 P-K308E-K309E-K312E, S163P-Q215K-S225G-N227T-N252P-N267T-H272V- F276A-S279G-Y281 P-K308E-K309E-K312E, S163P-Q215K-S225G-N227T-E232D-N252P-N267T- H272V-F276A-S279G-Y281 P-K308E-K309E-K312E and S163P-S186R-Q215K-S225G-N227T- E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E existing group, based on the numbering according to SEQ ID NO:1 .,

[0054] In particularly preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or 95% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, has at least 15 and increasingly preferably 16, 17, 18 or 19 amino acid substitutions, wherein the dispersin has an amino acid substitution combination which consists of the Q3F-T17W- A49W-N59E-S163P-S186R-S225G-N227T-G235W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P- H272V-F276A-S279G-K308E-K309E-K312E,Q3F-H15Y-V140I-S163P-S186R-Q215K-S225G- N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F- H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281 P- K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225G-N227T-G235W- S237W-N252P-H272P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-D207N- T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y- T17W-A49W-N59E-S163P-S186R-T218Q-S225G-N227T-G235W-S237W-N252P-H272P-Y281 P- K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-N227T-E232D-N252P-H272V-F276A- S279G-Y281 P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D- N252P-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-A49W-N59E-S163P-S186R-S225G- N227T-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F-S163P-S186R-Q215K- S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3F- S163P-S186R-Q215K-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E- K312E,Q3F-S163P-S186R-Q215K-S225G-N227T-N252P-N267T-H272V-F276A-S279G-Y281 P- K308E-K309E-K312E, Q3F-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P- N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312Q, Q3F-S163P-S186R-Q215K-S225G- N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312Q, Q3F-A49W- N59E-S163P-S186R-S225G-N227T-E232D-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E- K312E, Q3F-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G- Y281 P-K308E-K309E-K312E, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D- G235W-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-S163P-Q215K- S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, Q3F- H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D- Y281 P-K308Q-K309E-K312Q, Q3F-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272P-F276A-S279D-Y281 P-K308E-K309E-K312Q,Q3F-H15Y-T17W-S163P- S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E- K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N260Q-N267T-H272P- S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G-N227T-E232D- G235W-N252P-N267T-H272V-F276A-S279G-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-T17W- S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308Q- K309E-K312Q, Q3F-H15Y-A49W-N59E-V140I-S163P-S186R-Q215K-S225G-N227T-E232D-G235W- N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P- S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272P-S279D- Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312E, Q3I-H15Y-A49W-N59E-S163P-S186R- S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308E-K309E-K312Q,Q3I- H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D- Y281 P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272P-S279D-Y281 P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R- S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q, H15Y- A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-H272V-Y281 P-K308Q-K309E- K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V- S279D-Y281 P-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P- N260Q-H272P-S279D-Y281 P-S288P-K308Q-K309E-K312Q, H15Y-A49W-N59E-S163P-S186R- S225G-N227T-E232D-N252P-N260Q-H272P-S279D-Y281 P-K308Q-K309E-K312Q, H15Y-T17W- A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P- K308Q-K309E-K312Q, H15Y-T17W-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P- N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q,A49W-N59E-S163P-S186R-S225G-N227T- E232D-N252P-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E and S163P-S186R-Q215K- S225G-N227T-E232D-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E-K312E, based on the numbering according to SEQ ID NO:1.

[0055] In very particularly preferred embodiments, a dispersin is used which has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93% or 94% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length, has at least 18 amino acid substitutions, wherein the dispersin has an amino acid substitution combination which consists of the Q3F-H15Y-V140I-S163P-S186R-Q215K- S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-D207N-T218Q-S225G-N227T-G235W-S237W-N252P- H272P-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-T17W-A49W-N59E-S163P-S186R-T218Q-S225G- N227T-G235W-S237W-N252P-H272P-Y281 P-K308Q-K309E-K312Q,Q3F-T17W-S163P-S186R- Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281 P-K308E-K309E- K312Q, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-G235W-N252P-N260Q- N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S186R-Q215K-S225G- N227T-E232D-G235W-N252P-N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3F- A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272P-F276A-S279D- Y281 P-K308E-K309E-K312Q, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D- N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-S163P-S186R- Q215K-S225G-N227T-E232D-G235W-N252P-N267T-H272V-F276A-S279G-Y281 P-K308Q-K309E- K312Q, Q3F-H15Y-T17W-S163P-S186R-Q215K-S225G-N227T-E232D-N252P-N267T-H272V- F276A-S279G-Y281 P-K308Q-K309E-K312Q, Q3F-H15Y-A49W-N59E-V140I-S163P-S186R-Q215K- S225G-N227T-E232D-G235W-N252P-N260Q-N267T-H272V-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P-N260Q-N267T-H272V-S279D- Y281 P-K308Q-K309E-K312Q,Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-N252P- N260Q-N267T-H272P-S279D-Y281 P-K308Q-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R- S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312E, Q3I- H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D- Y281 P-K308E-K309E-K312Q, Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-N227T-E232D-G235W- N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q and H15Y-T17W-A49W-N59E-S163P- S186R-S225G-N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q existing group, based on the numbering according to SEQ ID NO:1.,

[0056] "β-1,6-N-acetylglucosamnidase activity," as used herein, is synonymous with "hexosaminidase activity" and / or "dispersin activity" and is determined as follows: To determine dispersin activity, 35 μl of dispersin assay solution (45 mM citrate buffer pH 5 with 0.5 mg / ml p-nitrophenyl-N-acetyl-β-D-glucosaminide) is added to a microtiter plate containing 5 μl of sample solution per well. The microtiter plate is incubated for 3 hours at room temperature. After incubation, 40 μl of stop solution (0.4 M Na2CO3) is added, and the absorbance is measured at 405 nm. The obtained activity values ​​are corrected by subtracting the absorbance measurement obtained for a reference without dispersin to eliminate background noise. From this, the residual activity (RA) for the respective dispersin variant can be calculated in comparison to a reference dispersin.

[0057] The enzymes mentioned herein are preferably mature enzymes, i.e., the catalytically active molecule without signal and / or propeptide(s). Unless otherwise stated, the sequences given also refer to mature (processed) enzymes.

[0058] In various embodiments of the invention, the respective enzyme is a free enzyme. This means that the enzyme can interact directly with all components of the agent and, if the agent is a liquid agent, that the enzyme is in direct contact with the agent's solvent (e.g., water). In other embodiments, an agent may contain enzymes that form an interaction complex with other molecules or that contain a "coat." In this case, a single or multiple enzyme molecules may be separated from the other components of the agent by a surrounding structure. Such a separating structure may be formed by, but is not limited to, vesicles, such as a micelle or a liposome. However, the surrounding structure may also be a virus particle, a bacterial cell, or a eukaryotic cell.In various embodiments, an agent may contain cells of Bacillus pumilus or Bacillus subtilus expressing the proteases of the invention, or cell culture supernatants of such cells.

[0059] In the context of the present invention, the feature that an enzyme has the indicated substitution(s) (or deletion or insertion) means that it contains one (of the indicated) substitution(s) (or deletion or insertion) at the respective position, i.e. at least the indicated positions are not otherwise mutated or deleted, for example by fragmentation of the enzyme. In various embodiments, the enzymes described herein have the sequence of the respective reference sequence, with the exception of the explicitly mentioned substitutions, i.e., apart from the substituted positions, they are 100% identical to the respective reference sequence.

[0060] The identity of nucleic acid or amino acid sequences is determined by sequence comparison. This sequence comparison is based on the BLAST algorithm, which is established and commonly used in the state of the art (see, for example, Altschul et al., Basic local alignment search tool, J. Mol. Biol., 1990, 215: 403-410, and Altschul et al., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res., 1997, 25: 3389-3402) and is essentially performed by matching similar sequences of nucleotides or amino acids in the nucleic acid or amino acid sequences to one another. A tabular assignment of the relevant positions is referred to as an alignment. Another algorithm available in the state of the art is the FASTA algorithm. Sequence comparisons (alignments), especially multiple sequence comparisons, are created using computer programs. Frequently used, for example, are the Clustal series (see e.g. Chenna et al., Multiple sequence alignment with the Clustal series of programs, Nucleic Acid Res., 2003, 31: 3497-3500), T-Coffee (cf. e.g. Notredame et al., T-Coffee: A novel method for multiple sequence alignments, J. Mol. Biol., 2000, 302: 205-217) or programs based on these programs or algorithms. Furthermore, sequence comparisons (alignments) are possible with the computer program Vector NTI® Suite 10.3 (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) with the predefined standard parameters, whose AlignX module for sequence comparisons is based on ClustalW, or Clone Manager 10 (use of the BLOSUM 62 scoring matrix for sequence alignment at the amino acid level). Unless otherwise stated, sequence identity reported herein is determined using the BLAST algorithm.

[0061] Such a comparison also allows a statement to be made about the similarity of the compared sequences to one another. This is usually expressed as percent identity, i.e. the proportion of identical nucleotides or amino acid residues at the same positions or corresponding positions in an alignment. The broader term homology in amino acid sequences includes conserved amino acid substitutions, i.e. amino acids with similar chemical activity, since these usually exert similar chemical activities within the protein. Therefore, the similarity of the compared sequences can also be expressed as percent homology or percent similarity. Identity and / or homology statements can be made for entire polypeptides or genes, or just for individual regions. Homologous or identical regions of different nucleic acid or amino acid sequences are therefore defined by similarities in the sequences.Such regions often exhibit identical functions. They can be small and comprise only a few nucleotides or amino acids. Such small regions often perform essential functions for the overall activity of the protein. It may therefore be useful to refer sequence matches only to individual, possibly small regions. Unless otherwise stated, however, statements of identity or homology in this application refer to the entire length of the respective nucleic acid or amino acid sequence.

[0062] In the context of the present invention, the statement that an amino acid position corresponds to a numerically designated position in SEQ ID NO:1 therefore means that the corresponding position is assigned to the numerically designated position in SEQ ID NO:1 in an alignment as defined above.

[0063] The following convention is used to describe substitutions that affect exactly one amino acid position (amino acid exchanges): first, the naturally occurring amino acid is named using the internationally accepted one-letter code, followed by the corresponding sequence position, and finally, the inserted amino acid. Multiple or alternative exchanges within the same polypeptide chain are separated by slashes. "130D / V" thus means that position 130 is mutated to D or V. In the case of insertions, additional amino acids are named after the sequence position. In the case of deletions, the missing amino acid is replaced by a symbol, such as an asterisk or a dash, or an A is indicated in front of the corresponding position.For example, P9T describes the substitution of proline at position 9 by threonine, P9TH the insertion of histidine after the amino acid threonine at position 9, and P9* or AP9 the deletion of proline at position 9. This nomenclature is known to those skilled in the art of enzyme technology.

[0064] Using methods commonly known today, such as chemical synthesis or the polymerase chain reaction (PCR) in conjunction with standard molecular biological and / or protein chemical methods, a person skilled in the art can produce the corresponding nucleic acids, including complete genes, based on known DNA and / or amino acid sequences. Such methods are known, for example, from Sambrook, J., Fritsch, EF, and Maniatis, T. (2001). Molecular cloning: a laboratory manual, 3rd Edition, Cold Spring Laboratory Press.

[0065] For the purposes of the invention, cleaning performance is understood to mean the ability of an agent to partially or completely remove existing soiling, in particular the brightening performance of one or more types of soiling on textiles. Furthermore, cleaning performance for the purposes of the present invention also includes the ability to remove microbially caused deposits, bio-fouling and the like from surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets. For the purposes of the invention, both the agent comprising enzymes, or the washing / cleaning liquor formed by this agent, and the respective enzyme itself have a respective cleaning performance.The enzyme's cleaning performance thus contributes to the cleaning performance of the agent or the washing / cleaning solution formed by the agent. The cleaning performance is preferably determined as specified below.

[0066] The term washing or cleaning liquor refers to the working solution containing the washing or cleaning agent that acts on the textiles or fabrics or surfaces, in particular dishes or household surfaces, and thus comes into contact with the soiling present on the textiles or fabrics or surfaces. The washing / cleaning liquor is usually created when the washing / cleaning process begins and the agent is diluted with water, e.g. in a washing machine or dishwasher or in another suitable container. The cleaning performance of textiles or fabrics can be determined in a washing system that contains a washing agent at a dosage of between 2.0 and 8.0 grams per liter of washing liquor and the enzyme to be tested. Enzymes to be compared are used at the same concentration (based on active protein).Using enzymes with equal activity ensures that the respective enzymatic properties, such as the cleaning performance on specific soils, can be compared even if there is a discrepancy in the ratio of active substance to total protein (the specific activity values). Generally speaking, a low specific activity can be compensated for by adding a larger amount of protein. Furthermore, the enzymes to be tested can also be used in the same amount of substance or weight if the enzymes to be tested exhibit different affinities for the test substrate in an activity test. The term "equal amount of substance" in this context refers to the same mole of the enzymes to be tested. The term "equal weight" refers to the same weight of the enzymes to be tested.

[0067] The concentration of the enzyme in the detergent intended for this washing system is 0.0001 to 0.1 wt.%, in particular 0.001 to 0.1 wt.%, more preferably 0.01 to 0.06 wt.% and particularly preferably 0.001 to 0.02 wt.%, based on active protein.

[0068] The protein concentration can be determined using known methods, for example, the BCA method (bicinchoninic acid; 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method (Gornall et al., J. Biol. Chem. 1948, 177: 751-766). The active protein concentration can be determined by titrating the active sites using a suitable irreversible inhibitor and determining the residual activity (cf. Bender et al., J. Am. Chem. Soc. 1966, 88, 24: 5890-5913).

[0069] A liquid reference detergent for such a washing system can, for example, be composed as follows (all data in weight percent (wt.%)): 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% C12-18 Na salts of fatty acids (soaps), 4.4% non-ionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamers, 2% glycerin, 0.08% preservatives, 1% ethanol, balance demineralized water. The dosage of the liquid detergent is preferably between 3.0 and 6.0 grams per liter of wash liquor, for example 3.0, 3.2, 3.5, 3.7, 4.0, 4.5, 4.7, 4.9 or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 7 and pH 10.5, preferably between pH 8 and pH 9.

[0070] The cleaning performance of a textile is determined by measuring the degree of cleanliness of the washed textiles. For example, the wash cycle can be carried out for 60 minutes at a temperature of 40°C and the water hardness can be between 15.5°dH and 16.5°dH (German hardness).

[0071] The degree of whiteness, i.e., the lightening of soils, as a measure of cleaning performance, is determined using optical measurement methods, preferably photometric. A suitable device for this purpose is the Minolta CM508d spectrometer. The devices used for the measurement are usually calibrated beforehand with a white standard, preferably one supplied with the product.

[0072] The cleaning performance on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, can be determined as described in Example 1.

[0073] Preferred embodiments of uses and agents according to the invention achieve such advantageous cleaning performance even at low temperatures, in particular in the temperature ranges between 10°C and 60°C, preferably between 15°C and 50°C, more preferably between 20°C and 40°C and particularly preferably 30°C.

[0074] In a preferred embodiment, the dispersin according to the invention, as described herein, can be used in a washing and / or cleaning agent. Such washing and cleaning agents according to the invention include all conceivable types of washing or cleaning agents, both concentrated and undiluted, for use on a commercial scale, in washing machines, or for hand washing or cleaning. These include, for example, detergents for textiles, carpets, or natural fibers, for which the term "detergent" is used.These include, for example, dishwashing detergents for dishwashers (machine dishwashing detergents) or manual dishwashing detergents or cleaners for hard surfaces such as metal, glass, porcelain, ceramics, tiles, stone, painted surfaces, plastics, wood or leather, for which the term cleaning agent is used, i.e. in addition to manual and machine dishwashing detergents, for example, also scouring agents, glass cleaners, toilet air fresheners, etc. The washing and cleaning agents within the scope of the invention also include washing aids which are added to the actual washing agent during manual or machine washing of textiles in order to achieve an additional effect.Furthermore, within the scope of the invention, washing and cleaning agents also include textile pre- and post-treatment agents, i.e., agents with which the laundry is brought into contact before the actual washing process, for example, to loosen stubborn soils, and also agents that, in a step following the actual textile washing process, impart further desirable properties to the laundry, such as a pleasant feel, crease resistance, or low static charge. Fabric softeners are considered among the latter. This also includes agents for use in (semi-)automated washing or cleaning systems, such as robotic mops or wet vacuum cleaners.

[0075] The detergents and / or cleaning agents according to the invention, which may be in the form of powdered or granular solids, in compacted or recompacted particle form, as homogeneous solutions or suspensions, may contain, in addition to the enzymes according to the invention, all known ingredients commonly found in such agents, with at least one further ingredient preferably being present in the agent. The agents according to the invention may contain, in particular, surfactants, builders, complexing agents, polymers, glass corrosion inhibitors, corrosion inhibitors, bleaching agents such as peroxygen compounds, bleach activators or bleach catalysts, water-miscible organic solvents, enzyme stabilizers, sequestering agents, electrolytes, pH regulators, and / or other auxiliaries such as optical brighteners, graying inhibitors, dye transfer inhibitors, foam regulators, as well as dyes and fragrances.Advantageous ingredients of the compositions according to the invention are disclosed in the international patent application WO 2009 / 121725, beginning on page 5, penultimate paragraph, and ending on page 13 after the second paragraph. This disclosure is expressly incorporated by reference, and the disclosure content therein is incorporated into the present patent application.

[0076] An agent according to the invention advantageously contains the enzymes according to the invention in an amount of from 2 pg to 20 mg, preferably from 5 pg to 17.5 mg, particularly preferably from 20 pg to 15 mg and very particularly preferably from 50 pg to 10 mg per g of the agent.

[0077] An agent according to the invention contains the enzymes according to the invention increasingly preferably in an amount of 1 x 10 -8 up to 5 wt.%, from 0.0001 to 1 wt.%, from 0.0005 to 0.5 wt.%, from 0.001 to 0.1 wt.%, each based on active protein and based on the total weight of the agent.

[0078] In preferred embodiments, a washing and / or cleaning agent according to the invention contains the dispersin in an amount of 0.01 to 5 wt.%, preferably 0.1 to 4 wt.%, particularly preferably 0.15 to 2.5 wt.%, based on the total weight of the agent.

[0079] In preferred embodiments, a washing and / or cleaning agent according to the invention contains the at least three further enzymes selected from the group consisting of amylase, protease, lipase, cellulase, mannanase, pectate lyase and mixtures thereof, in a total amount of 0.01 to 10% by weight, preferably 0.1 to 8% by weight, particularly preferably 0.2 to 6% by weight, based on the total weight of the agent.

[0080] Furthermore, the enzymes contained in the agent and / or other ingredients of the agent can be coated with a substance that is impermeable to the enzyme at room temperature or in the absence of water, which substance becomes permeable to the enzyme under the conditions of use of the agent. Such an embodiment of the invention is thus characterized in that the enzyme is coated with a substance that is impermeable to the enzyme at room temperature or in the absence of water. Furthermore, the agent itself can also be packaged in a container, preferably an air-permeable container, from which it is released shortly before use or during the washing process.

[0081] Further embodiments of the invention include all solid, powdered, liquid, gel-like, or pasty dosage forms of agents according to the invention, which may optionally also consist of multiple phases and may be in compressed or uncompressed form. The agent may be in the form of a free-flowing powder, in particular with a bulk density of 300 g / l to 1200 g / l, in particular 500 g / l to 900 g / l or 600 g / l to 850 g / l. Solid dosage forms of the agent also include extrudates, granules, tablets, or pouches. Alternatively, the agent may also be liquid, gel-like, or pasty, for example in the form of a non-aqueous liquid detergent or a non-aqueous paste, or in the form of an aqueous liquid detergent or a water-containing paste. Liquid agents are generally preferred. Furthermore, the agent may be in the form of a one-component system. Such agents consist of one phase.Alternatively, a remedy can also consist of multiple phases. Such a remedy is therefore divided into several components.

[0082] In a preferred embodiment, the agent according to the invention is a textile detergent.

[0083] In a further preferred embodiment, the agent according to the invention is a liquid textile detergent.

[0084] In a particularly preferred embodiment, the agent according to the invention is a pre-portioned detergent, in particular a detergent portion unit comprising a detergent preparation according to the invention and a water-soluble film which completely encloses the detergent preparation.

[0085] The water-soluble film in which the detergent preparation is packaged can comprise one or more structurally different water-soluble^) polymer(s). Particularly suitable water-soluble polymer(s) are polymers from the group of (optionally acetalized) polyvinyl alcohols (PVAL) and their copolymers. Water-soluble films are preferably based on a polyvinyl alcohol or a polyvinyl alcohol copolymer whose molecular weight is in the range from 10,000 to 1,000,000 g / mol, preferably from 20,000 to 500,000 g / mol, particularly preferably from 30,000 to 100,000 g / mol and in particular from 40,000 to 80,000 g / mol. Suitable water-soluble films for use are marketed, among others, by MonoSol LLC, for example under the designation M8630, M8720, M8310, C8400 or M8900. Also suitable are films called Solublon® PT, Solublon® GA, Solublon® KC or Solublon® KL from Aicello Chemical Europe GmbH or the VF-HP films from Kuraray.

[0086] When the detergents according to the invention are in liquid form, they preferably contain, based on their total weight, more than 40% by weight, preferably 50 to 90% by weight and particularly preferably 60 to 80% by weight of water.

[0087] The agents according to the invention may contain one or more surfactants, with particular preference being given to anionic surfactants, nonionic surfactants, and mixtures thereof, but cationic, zwitterionic, and / or amphoteric surfactants may also be present. The agents preferably contain 5 to 70% by weight of surfactant, more preferably 35 to 60% by weight, and more preferably 40 to 55% by weight of surfactant.

[0088] Suitable anionic surfactants are, in particular, soaps and those containing sulfate or sulfonate groups, preferably with alkali ions as cations. Suitable soaps are preferably the alkali salts of saturated or unsaturated C12-C18 fatty acids. Such fatty acids can also be used in a non-neutralized form. Suitable sulfate-type surfactants include the salts of the sulfuric acid monoesters of C12-C18 fatty alcohols and the sulfation products of the aforementioned nonionic surfactants with a low degree of ethoxylation. The usable surfactants of the sulfonate type include, for example, Cg-u-alkylbenzenesulfonates, alkanesulfonates obtained from Ci2-is-alkanes, for example by sulfochlorination or sulfoxidation with subsequent hydrolysis or neutralization, Ci2-18-olefinsulfonates, which are formed by the reaction of corresponding monoolefins with sulfur trioxide, mixtures of alkene and hydroxyalkanesulfonates, disulfonates, as can be found, for example, infrom Ci2-18 monoolefins with terminal or internal double bonds by sulfonation with gaseous sulfur trioxide and subsequent alkaline or acidic hydrolysis of the sulfonation products, as well as a-sulfofatty acid esters (ester sulfonates) which are formed during the sulfonation of fatty acid methyl or ethyl esters, e.g. a-sulfonated methyl esters of hydrogenated coconut, palm kernel or tallow fatty acids.

[0089] The agent preferably contains 2 to 55 wt.%, preferably 3 to 35 wt.%, of anionic surfactant. Most preferably, the agent contains 3 to 25 wt.% alkylbenzenesulfonate. Furthermore, the agent may preferably contain other anionic surfactants, especially alkyl ether sulfates, as well as nonionic surfactants, especially fatty alcohol alkoxylates. These can then make up the remainder of the surfactants.

[0090] Suitable alkylbenzenesulfonates are preferably selected from linear or branched alkylbenzenesulfonates of the formula in which R' and R" are independently H or alkyl and together contain 6 to 19, preferably 7 to 15 and in particular 9 to 13 C atoms. A particularly preferred representative is sodium dodecylbenzylsulfonate.

[0091] Preferred alk(en)yl sulfates are the alkali metal salts, especially the sodium salts, of the sulfuric acid half-esters of C12-18 fatty alcohols, e.g., coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or C18-20 oxo alcohols, and those half-esters of secondary alcohols of these chain lengths. Also preferred are alk(en)yl sulfates of the stated chain length that contain a synthetic, petrochemically produced straight-chain alkyl radical, which exhibit degradation behavior similar to that of equivalent compounds based on oleochemical raw materials. For washing purposes, C12-16 alkyl sulfates and C12-15 alkyl sulfates, as well as C11-18 alkyl sulfates, are preferred.

[0092] Also suitable are the sulfuric acid monoesters of straight-chain or branched C1-2i alcohols ethoxylated with 1 to 6 mol of ethylene oxide, such as 2-methyl-branched C1-n alcohols with an average of 3.5 mol of ethylene oxide (EO) or C1-2is fatty alcohols with 1 to 4 EO.

[0093] Suitable alkyl ether sulfates are, for example, compounds of the formula R 1 -O-(AO) n -SO3- X + .

[0094] In this formula, R 1 represents a linear or branched, substituted or unsubstituted alkyl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical. Preferred radicals R 1 are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals and mixtures thereof, with the representatives having an even number of carbon atoms being preferred. Particularly preferred radicals R 1are derived from C12-18 fatty alcohols, e.g., from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or from C12-20 oxo alcohols. AO stands for an ethylene oxide (EO) or propylene oxide (PO) group, preferably an ethylene oxide group. The index n stands for an integer from 1 to 50, preferably from 1 to 20, and in particular from 2 to 10. Most preferably, n stands for the numbers 2, 3, 4, 5, 6, 7, or 8. X + stands for a monovalent cation or the n-th part of an n-valent cation, preferably the alkali metal ions and among them Na + or K + , where Na + is highly preferred. Other cations X + can be selected from NE T % Zn 2+ , % Mg 2+ , % Ca 2+ , % Mn 2+ and their mixtures.

[0095] In various embodiments, the alkyl ether sulfate may be selected from fatty alcohol ether sulfates of the formula with k = 1 to 19, n = 2, 3, 4, 5, 6, 7, or 8. Very particularly preferred representatives are Na-Ci2-14 fatty alcohol ether sulfates with 2 EO (k = 1 to 13, n = 2). The stated degree of ethoxylation represents a statistical mean, which can be a whole or fractional number for a specific product. The stated degrees of alkoxylation represent statistical mean, which can be a whole or fractional number for a specific product. Preferred alkoxylates / ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE).

[0096] It has proven beneficial for cold-wash performance if the detergents also contain soap(s). Preferred detergents are therefore characterized by the presence of soap(s). Suitable soaps include saturated fatty acid soaps, such as the salts of lauric acid, myristic acid, palmitic acid, stearic acid, hydrogenated erucic acid, and behenic acid, as well as, in particular, soap mixtures derived from natural fatty acids, e.g., coconut, palm kernel, or tallow fatty acids.

[0097] Suitable nonionic surfactants are, in particular, alkyl glycosides and ethoxylation and / or propoxylation products of alkyl glycosides or linear or branched alcohols, each containing 8 to about 18 carbon atoms in the alkyl moiety and 3 to 20, preferably 4 to 10, alkyl ether groups. Furthermore, corresponding ethoxylation and / or propoxylation products of N-alkylamines, vicinal diols, fatty acid esters, and fatty acid amides, which correspond to the aforementioned long-chain alcohol derivatives with regard to the alkyl moiety, as well as of alkylphenols with 5 to 12 carbon atoms in the alkyl radical, are also suitable. As non-ionic surfactants, preferably alkoxylated, advantageously ethoxylated, in particular primary alcohols with preferably 8 to 18 C atoms and an average of 1 to 12 moles of ethylene oxide (EO) per mole of alcohol are used, in which the alcohol radical can be linear or preferably methyl-branched in the 2-position.linear and methyl-branched radicals in the mixture, as are usually found in oxo alcohol radicals. However, alcohol ethoxylates with linear radicals from alcohols of native origin with 12 to 18 C atoms, e.g. from coconut, palm, tallow or oleyl alcohol, and an average of 2 to 8 EO per mole of alcohol are particularly preferred. The preferred ethoxylated alcohols include, for example, C12-14 alcohols with 3 EO or 4 EO, C8-n alcohol with 7 EO, C11-18 alcohols with 3 EO, 5 EO, 7 EO or 8 EO, C12-18 alcohols with 3 EO, 5 EO or 7 EO and mixtures of these, such as mixtures of C12-14 alcohol with 3 EO and C12-18 alcohol with 5 EO. The stated degrees of ethoxylation represent statistical averages, which can be a whole or fractional number for a specific product. Preferred alcohol ethoxylates exhibit a narrow homolog distribution (narrow range ethoxylates, NRE).In addition to these nonionic surfactants, fatty alcohols with more than 12 EO can also be used. Examples include tallow fatty alcohol with 14 EO, 25 EO, 30 EO, or 40 EO.

[0098] Another class of preferably used nonionic surfactants, which are used either as the sole nonionic surfactant or in combination with other nonionic surfactants, are alkoxylated, preferably ethoxylated or ethoxylated and propoxylated fatty acid alkyl esters, preferably having 1 to 4 carbon atoms in the alkyl chain, in particular fatty acid methyl esters.

[0099] Another class of nonionic surfactants that can be used advantageously are alkyl polyglycosides (APG). Suitable alkyl polyglycosides satisfy the general formula

[0100] RO(G)z, in which R is a linear or branched, in particular 2-methyl-branched, saturated or unsaturated, aliphatic radical having 8 to 22, preferably 12 to 18, carbon atoms, and G is the symbol representing a glycose unit having 5 or 6 carbon atoms, preferably glucose. The degree of glycosidation z is between 1 and 4, preferably between 1 and 2, and in particular between 1.1 and 1.4. Preference is given to using linear alkyl polyglycosides, i.e. alkyl polyglycosides in which the polyglycosyl radical is a glucose radical and the alkyl radical is an n-alkyl radical.

[0101] Nonionic surfactants of the amine oxide type, e.g., N-cocoalkyl-N,N-dimethylamine oxide and N-tallowalkyl-N,N-dihydroxyethylamine oxide, and fatty acid alkanolamides may also be suitable. The amount of these nonionic surfactants is preferably no more than that of the ethoxylated fatty alcohols, in particular no more than half that amount.

[0102] Suitable amphoteric surfactants are, for example, betaines of the formula

[0103] (R iii )(R iv )(R v )N + CH2COO-, in which R'" is an alkyl radical having 8 to 25, preferably 10 to 21 carbon atoms, optionally interrupted by heteroatoms or heteroatom groups, and R iv and R v identical or different alkyl radicals having 1 to 3 carbon atoms, in particular C10-18-

[0104] Alkyldimethylcarboxymethylbetaine and Cn-17-alkylamidopropyldimethylcarboxymethylbetaine.

[0105] Suitable cationic surfactants include the quaternary ammonium compounds of the formula

[0106] (Rvi)(Rvü)( R ™)( R ^ N + X in the R vi to R ixrepresents four identical or different, in particular two long- and two short-chain, alkyl radicals, and X- represents an anion, in particular a halide ion, e.g., didecyldimethylammonium chloride, alkylbenzyldidecylammonium chloride, and mixtures thereof. Other suitable cationic surfactants are quaternary surface-active compounds, in particular those containing a sulfonium, phosphonium, iodonium, or arsonium group, which are also known as antimicrobial agents. By using quaternary surface-active compounds with antimicrobial activity, the agent can be given an antimicrobial effect or its existing antimicrobial effect, possibly due to other ingredients, can be improved.

[0107] Another preferred component of agents according to the invention are complexing agents. Particularly preferred complexing agents are phosphonates, provided their use is permitted by regulations. In addition to 1-hydroxyethane-1,1-diphosphonic acid, the complexing phosphonates include a number of different compounds, such as diethylenetriaminepenta(methylenephosphonic acid) (DTPMP). Hydroxyalkane and aminoalkanephosphonates are particularly preferred in this application. Among the hydroxyalkanephosphonates, 1-hydroxyethane-1,1-diphosphonate (HEDP) is of particular importance as a cobuilder. It is preferably used as the sodium salt, with the disodium salt being neutral and the tetrasodium salt being alkaline (pH 9). Preferred aminoalkanephosphonates are ethylenediaminetetramethylenephosphonate (EDTMP), diethylenetriaminepentamethylenephosphonate (DTPMP), and their higher homologues. They are preferably used in the form of neutral sodium salts, e.g.as the hexasodium salt of EDTMP or as the hepta- and octa-sodium salt of DTPMP. HEDP is preferably used as a builder from the phosphonate class. Aminoalkanephosphonates also possess a pronounced heavy metal binding capacity. Accordingly, it may be preferable, particularly if the agents also contain bleach, to use aminoalkanephosphonates, in particular DTPMP, or to use mixtures of the phosphonates mentioned. A preferred agent in the context of this application contains one or more phosphonate(s) from the group consisting of aminotrimethylenephosphonic acid (ATMP) and / or salts thereof; ethylenediaminetetra(methylenephosphonic acid) (EDTMP) and / or salts thereof;.

[0108] Diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) and / or its salts; 1-hydroxyethane-1,1-diphosphonic acid (HEDP) and / or its salts; 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC) and / or its salts; hexamethylenediaminetetra(methylenephosphonic acid) (HDTMP) and / or its salts; nitrilotri(methylenephosphonic acid) (NTMP) and / or its salts. Particularly preferred agents are those containing 1-hydroxyethane-1,1-diphosphonic acid (HEDP) or diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) as phosphonates. Of course, the agents according to the invention can contain two or more different phosphonates. Preferred agents according to the invention are characterized in that the agent contains at least one complexing agent from the group of phosphonates, preferably 1-hydroxyethane-1,1-diphosphonate, wherein the weight proportion of the phosphonate in the total weight of the agent is preferably 0.1 to 8.0 wt.%, preferably 0.2 to 5.0 wt.%, more preferably 0.3 to 3.0 wt.-% and particularly preferably 0.5-2.0 wt.%.

[0109] In further preferred embodiments, the washing and / or cleaning agents according to the invention are substantially free of phosphonate-containing compounds. "Substantially free of phosphonate-containing compounds" in this context means that the corresponding agents contain less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight, and particularly preferably less than 0.1% by weight, of phosphonate-containing compounds, based on the total weight of the agent. In particularly preferred embodiments, these agents are free of phosphonate-containing compounds.

[0110] The compositions according to the invention preferably further contain builders, preferably at least one water-soluble and / or water-insoluble, organic and / or inorganic builder. Builders include, in particular, silicates, carbonates, and organic cobuilders.

[0111] Organic cobuilders include, in particular, polycarboxylates / polycarboxylic acids, polymeric polycarboxylates, aspartic acid, polyacetals, dextrins, other organic cobuilders, and phosphonates. These classes of substances are described below. Organic cobuilder substances can, if desired, be present in amounts of up to 40 wt.%, in particular up to 25 wt.%, and preferably from 1 to 8 wt.%. Suitable organic builders include, for example, polycarboxylic acids, which can be used in the form of the free acid and / or their sodium salts. Polycarboxylic acids are understood to be carboxylic acids that carry more than one acid function.For example, these are citric acid, adipic acid, succinic acid, glutaric acid, malic acid, tartaric acid, maleic acid, fumaric acid, sugar acids and carboxymethyl inulins, monomeric and polymeric aminopolycarboxylic acids, in particular glycinediacetic acid, methylglycinediacetic acid, glutaminediacetic acid, nitrilotriacetic acid (NTA), iminodisuccinate such as ethylenediamine-N,N'-disuccinic acid and hydroxyiminodisuccinates, ethylenediaminetetraacetic acid and polyaspartic acid, polyphosphonic acids, in particular aminotris(methylenephosphonic acid), ethylenediaminetetrakis(methylenephosphonic acid), lysinetetra(methylenephosphonic acid) and 1-hydroxyethane-1,1-diphosphonic acid, polymeric hydroxy compounds such as dextrin and polymeric (poly)carboxylic acids, in particular by oxidation of polysaccharides orDextrin-accessible polycarboxylates, and / or polymeric acrylic acids, methacrylic acids, maleic acids, and copolymers thereof, which may also contain small amounts of polymerizable substances without carboxylic acid functionality. Such organic builder substances can, if desired, be present in amounts of up to 50% by weight, in particular up to 25% by weight, preferably from 10 to 20% by weight, and particularly preferably from 1 to 5% by weight. In addition to their builder effect, the free acids typically also have the property of an acidifying component and thus also serve to adjust a lower and milder pH value of agents. Particular mention should be made of citric acid, succinic acid, glutaric acid, adipic acid, gluconic acid, and any mixtures thereof. Citric acid or salts of citric acid are particularly preferably used as the builder substance.Further particularly preferred builders are selected from methylglycinediside acid (MGDA), glutamic acid diacetate (GLDA), aspartic acid diacetate (ASDA), hydroxyethyliminodiacetate (HEIDA), iminodisuccinate (IDS), ethylenediamine disuccinate (EDDS), carboxymethylinulin, and polyaspartate. In preferred embodiments, citric acid and / or citrate are used as the water-soluble, organic builder. Particular preference is given to using 0.5 to 25 wt.%, preferably 0.75 to 12.5 wt.%, more preferably 1 to 4 wt.% of citric acid and / or 0.5 to 25 wt.%, preferably 0.75 to 12.5 wt.%, more preferably 1 to 4 wt.% of citrate, preferably alkali metal citrate, even more preferably sodium citrate. Citric acid / citrate can each be used in the form of their hydrates, for example citric acid can be used in the form of the monohydrate, citrate in the form of trisodium citrate dihydrate.

[0112] Other suitable builders include polymeric polycarboxylates, for example the alkali metal salts of polyacrylic acid or polymethacrylic acid, e.g. those with a relative molecular mass of 500 to 70,000 g / mol. For the purposes of this application, the molecular masses stated for polymeric polycarboxylates are weight-average molecular masses Mw of the respective acid form, which were generally determined by gel permeation chromatography (GPC) using a UV detector. The measurement was carried out against an external polyacrylic acid standard, which, due to its structural similarity to the polymers investigated, provides realistic molecular weight values. These figures differ significantly from the molecular weight figures used when polystyrenesulfonic acids are used as the standard. The molecular masses measured against polystyrenesulfonic acids are generally significantly higher than the molecular masses stated in this application.Suitable polymers are, in particular, polyacrylates, which preferably have a molecular weight of 2000 to 20,000 g / mol. Due to their superior solubility, the short-chain polyacrylates, which have molecular weights of 2000 to 10,000 g / mol, and particularly preferably of 3000 to 5000 g / mol, may be preferred from this group. Also suitable are copolymers of polycarboxylates, in particular those of acrylic acid with methacrylic acid and of acrylic acid or methacrylic acid with maleic acid. Copolymers of acrylic acid with maleic acid, which contain 50 to 90 wt.% acrylic acid and 50 to 10 wt.% maleic acid, have proven particularly suitable. Their relative molecular weight, based on free acids, is generally 2000 to 70,000 g / mol, preferably 20,000 to 50,000 g / mol, and in particular 30,000 to 40,000 g / mol.

[0113] A solid composition according to the invention preferably contains at least one water-soluble and / or water-insoluble, organic and / or inorganic builder. The water-soluble organic builder substances include the above-mentioned organic builders.

[0114] In addition to the aforementioned water-soluble organic builders, the agents of the invention may also contain inorganic water-soluble builders. Suitable water-soluble inorganic builder materials include, in particular, alkali metal silicates, alkali metal carbonates, alkali metal hydrogen carbonates, alkali metal phosphates, and / or sesquicarbonates, which may be in the form of their alkaline, neutral, or acidic sodium or potassium salts. Small amounts of calcium carbonates may also be present in solid textile detergents if desired. Suitable examples include water-soluble crystalline and / or amorphous alkali metal silicates. The alkali metal silicates usable as builders in the agents according to the invention preferably have a molar ratio of alkali metal oxide to SiO2 of less than 0.95, in particular of 1:1.1 to 1:12, and may be amorphous or crystalline.Preferred alkali silicates are sodium silicates, especially amorphous sodium silicates, with a molar ratio of Na2O:SiO2 of 1:2 to 1:2.8. Crystalline silicates, which can be present alone or in a mixture with amorphous silicates, are preferably crystalline layered silicates of the general formula Na2Si. xO2x+i • y H2O is used, in which x, the so-called modulus, is a number from 1.9 to 22, in particular 1.9 to 4 and y is a number from 0 to 33 and preferred values ​​for x are 2, 3 or 4. Preferred crystalline layered silicates are those in which x in the general formula mentioned takes on the values ​​2 or 3. In particular, both β- and δ-sodium disilicates (Na2Si2Os • y H2O) are preferred. Practically anhydrous crystalline alkali silicates produced from amorphous alkali silicates of the above general formula, in which x is a number from 1.9 to 2.1, can also be used in agents according to the invention. In a further embodiment of agents according to the invention, a crystalline sodium layered silicate with a modulus of 2 to 3 is used, such as can be produced from sand and soda. Crystalline sodium silicates with a modulus in the range of 1.9 to 3.5 are used in a further embodiment of the compositions according to the invention.In agents containing both amorphous and crystalline alkali silicates, the weight ratio of amorphous alkali silicate to crystalline alkali silicate is preferably 1:2 to 2:1 and in particular 1:1 to 2:1. Crystalline layered silicates of the above-mentioned formula (I) are marketed by Clariant GmbH under the trade name Na-SKS, e.g. Na-SKS-1 (Na2Si2O45 • x H2O, Kenyaite), Na-SKS-2 (Na2SiO29 • x H2O, Magadiite), Na-SKS-3 (Na2SiO17 • x H2O) or Na-SKS-4 (Na2SiO4Og • x H2O, Makatite). Of these, Na-SKS-5 (α-Na2Si2O5), Na-SKS-7 (β-Na2Si2O5, Natrosilite), Na-SKS-9 (NaHSi2O5• 3 H2O), Na-SKS-10 (NaHSi2O5• 3 H2O, Kanemite), Na-SKS-11 (β-Na2Si2O5) and Na-SKS-13 (NaHSi2O5), but especially Na-SKS-6 (δ-Na2Si2O5) are particularly suitable.In one embodiment of the inventive compositions, a granular compound of crystalline phyllosilicate and citrate, of crystalline phyllosilicate and the above-mentioned (co)polymeric polycarboxylic acid, or of alkali silicate and alkali carbonate is used, as is commercially available, for example, under the name Nabion® 15. Such water-soluble inorganic builder materials are preferably present in the compositions according to the invention in amounts of 1 to 20 wt.%, in particular 5 to 15 wt. Other important water-soluble inorganic builder substances are carbonates (and hydrocarbonates), in particular sodium carbonate, and phosphonic acids / phosphonates.

[0115] The agents according to the invention are preferably free of phosphate builders, i.e., they contain less than 1% by weight, preferably no deliberately added phosphate builder. The agents may also contain water-insoluble builders. Crystalline or amorphous, water-dispersible alkali aluminosilicates are used as water-insoluble inorganic builder materials, in particular in amounts of up to 50% by weight, preferably not more than 40% by weight, in particular from 3 to 20% by weight, and particularly preferably from 1 to 15% by weight. Among these, crystalline sodium aluminosilicates of detergent quality are preferred, in particular zeolite A, zeolite P, zeolite MAP, and optionally zeolite X, alone or in mixtures, e.g., in the form of a co-crystallizate of zeolites A and X (Vegobond® AX, a commercial product of Condea Augusta SpA). Amounts close to the stated upper limit are preferably used in solid, particulate agents.Suitable aluminosilicates, in particular, do not contain any particles with a grain size larger than 30 pm and preferably consist of at least 80 wt.% particles with a size smaller than 10 pm. Their calcium binding capacity, which can be determined according to DE 2412837 A1, is generally in the range of 100 to 200 mg CaO per gram.

[0116] In addition to the previously described builders, the agent may contain cleaning-active polymers. The weight fraction of the cleaning-active polymers in the total weight of the agent according to the invention is preferably 0.1 to 20 wt.%, preferably 1.0 to 15 wt.%, and more preferably 2.0 to 12 wt.%.

[0117] Peroxygen compounds suitable for use in agents according to the invention include, in particular, organic peracids or peracidic salts of organic acids, such as phthalimidopercaproic acid, perbenzoic acid, or salts of diperdodecanedioic acid, hydrogen peroxide, and inorganic salts that release hydrogen peroxide under washing conditions, including perborate, percarbonate, persilicate, and / or persulfate such as caroate, as well as hydrogen peroxide inclusion compounds, such as H2G2-urea adducts. Hydrogen peroxide can also be produced with the aid of an enzymatic system, i.e., an oxidase and its substrate. If solid peroxygen compounds are to be used, they can be used in the form of powders or granules, which can also be coated in a manner known in principle.The peroxygen compounds can be added to the wash liquor as such or in the form of agents containing them, which in principle can contain all conventional washing, cleaning, or disinfectant ingredients. Particular preference is given to using alkali metal percarbonate or alkali metal perborate monohydrate. If an agent according to the invention contains peroxygen compounds, they are present in amounts of preferably up to 50 wt.%, in particular from 5 to 30 wt.%, more preferably from 0.1 to 20 wt.%.

[0118] Compounds that, under perhydrolysis conditions, yield aliphatic peroxocarboxylic acids with preferably 1 to 10 carbon atoms, in particular 2 to 4 carbon atoms, and / or optionally substituted perbenzoic acid can be used as bleach activators in the detergents. Suitable substances are those that carry O- and / or N-acyl groups with the stated number of carbon atoms and / or optionally substituted benzoyl groups. Preferred are multiply acylated alkylenediamines, in particular tetraacetylethylenediamine (TAED), acylated triazine derivatives, in particular 1,5-diacetyl-2,4-dioxohexahydro-1,3,5-triazine (DADHT), acylated glycolurils, in particular tetraacetylglycoluril (TAGU), N-acylimides, in particular N-nonanoylsuccinimide (NOSI), acylated phenolsulfonates or carboxylates or the sulfonic or carboxylic acids thereof, in particular nonanoyl or isononanoyloxybenzenesulfonate or laroyloxybenzenesulfonate (NOBS or iso-NOBS orLOBS), 4-(2-decanoyloxyethoxycarbonyloxy)-benzenesulfonate (DECOBS) or decanoyloxybenzoate (DOBA), carboxylic acid anhydrides, in particular phthalic anhydride, acylated polyhydric alcohols, in particular triacetin, ethylene glycol diacetate, 2,5-diacetoxy-2,5-dihydrofuran and enol esters as well as acetylated sorbitol and mannitol or their described mixtures (SORMAN), acylated sugar derivatives, in particular pentaacetylglucose (PAG), pentaacetylfructose, tetraacetylxylose and octaacetyllactose, acetylated, optionally N-alkylated glucamine and gluconolactone, N-acylated lactams, e.g. N-benzoylcaprolactam, nitriles from which perimidic acids are formed, in particular aminoacetonitrile derivatives with a quaternized nitrogen atom, and / or Oxygen-transferring sulfonimines and / or acylhydrazones. Hydrophilically substituted acyl acetals and acyl lactams are also preferred. Combinations of conventional bleach activators can also be used.Such bleach activators can be present, particularly in the presence of the above-mentioned hydrogen peroxide-providing bleaching agents, in the usual amount range, preferably in amounts of 0.5 to 10 wt.%, in particular 1 to 8 wt.%, based on the total agent, but are preferably completely absent when percarboxylic acid is used as the sole bleaching agent.

[0119] In addition to the conventional bleach activators or instead of them, solid agents may also contain sulfonimines and / or bleach-enhancing transition metal salts or transition metal complexes as so-called bleach catalysts.

[0120] Suitable graying inhibitors or soil-release agents (soil-release polymers) are cellulose ethers, such as carboxymethylcellulose, methylcellulose, hydroxyalkylcelluloses, and cellulose mixed ethers, such as methylhydroxyethylcellulose, methylhydroxypropylcellulose, and methylcarboxymethylcellulose. Preference is given to using sodium carboxymethylcellulose, hydroxypropylmethylcellulose, and mixtures thereof, and optionally mixtures thereof with methylcellulose. Commonly used soil-release agents include copolyesters containing dicarboxylic acid units, alkylene glycol units, and polyalkylene glycol units. The proportion of graying inhibitors and / or soil-release agents in agents according to the invention generally does not exceed 2% by weight and is preferably 0.5 to 1.5% by weight, particularly preferably 0.5 to 2% by weight.

[0121] Optical brighteners, particularly for textiles made from cellulose fibers (e.g., cotton), may include derivatives of diaminostilbene disulfonic acid or its alkali metal salts. Suitable brighteners include, for example, salts of 4,4'-bis(2-anilino-4-morpholino-1,3,5-triazin-6-ylamino)stilbene-2,2'-disulfonic acid or similarly structured compounds that carry a diethanolamino group, a methylamino group, or a 2-methoxyethylamino group instead of the morpholino group. Furthermore, brighteners of the substituted 4,4'-distyryl-diphenyl type may be present, e.g., 4,4'-bis(4-chloro-3-sulfostyryl)-diphenyl. Mixtures of brighteners can also be used. Brighteners of the 1,3-diaryl-2-pyrazoline type, e.g., 1-(p-sulfoamoylphenyl)-3-(p-chlorophenyl)-2-pyrazoline, and similarly structured compounds, are particularly suitable for polyamide fibers. The optical brightener or brightener mixture content of the agent generally does not exceed 1 wt.-%, preferably 0.05 to 0.5 wt.%. In a preferred embodiment of the invention, the agent is free of such active ingredients.

[0122] The conventional foam regulators that can be used in the agents according to the invention include, for example, polysiloxane-silica mixtures, the finely divided silica contained therein preferably being silanized or otherwise hydrophobized. The polysiloxanes can consist of linear compounds as well as crosslinked polysiloxane resins and mixtures thereof. Other defoamers are paraffin hydrocarbons, in particular microparaffins and paraffin waxes, whose melting point is above 40°C, saturated fatty acids or soaps with, in particular, 20 to 22 C atoms, e.g., sodium behenate, and alkali metal salts of phosphoric acid mono- and / or dialkyl esters in which the alkyl chains each have 12 to 22 C atoms. Among these, sodium monoalkyl phosphate and / or dialkyl phosphate with C 16-18 alkyl groups is preferably used. The proportion of foam regulators can preferably be 0.2 to 2 wt.%, particularly preferably not more than 1 wt.%.

[0123] To adjust the desired pH, the agents according to the invention can contain system- and environmentally compatible acids, in particular citric acid, acetic acid, tartaric acid, malic acid, lactic acid, glycolic acid, succinic acid, glutaric acid, and / or adipic acid, but also mineral acids, in particular sulfuric acid or alkali metal hydrogen sulfates, or bases, in particular ammonium or alkali metal hydroxides, preferably sodium hydroxide. Such pH regulators are preferably present in the agents according to the invention in amounts of no more than 10% by weight, in particular from 0.5 to 6% by weight, particularly preferably from 0.3 to 2% by weight.

[0124] The agents according to the invention may contain an organic solvent as a further component. The addition of organic solvents has a beneficial effect on the enzyme stability and cleaning performance of these agents. Preferred organic solvents come from the group of mono- or polyhydric alcohols, alkanolamines, or glycol ethers.The solvents are preferably selected from ethanol, n- or i-propanol, butanol, glycol, propanediol, butanediol, glycerin, diglycol, propyl diglycol, butyl diglycol, hexylene glycol, ethylene glycol methyl ether, ethylene glycol ethyl ether, ethylene glycol propyl ether, ethylene glycol mono-n-butyl ether, diethylene glycol methyl ether, diethylene glycol ethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, propylene glycol propyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, methoxytriglycol, ethoxytriglycol, butoxytriglycol, 1-butoxyethoxy-2-propanol, 3-methyl-3-methoxybutanol, propylene glycol t-butyl ether and mixtures of these solvents. The weight proportion of these organic solvents in the total weight of the compositions according to the invention is preferably 0.1 to 10 wt.%, preferably 0.2 to 8.0 wt.% and more preferably 0.5 to 5.0 wt.%.A particularly preferred organic solvent that is particularly effective with regard to stabilizing the agents is glycerol and 1,2-propylene glycol. Liquid agents preferably comprise at least one polyol, preferably from the group consisting of glycerol and 1,2-propylene glycol, in an amount of 0.1 to 10% by weight, preferably 0.2 to 8.0% by weight, and more preferably 0.5 to 5.0% by weight, based on the total weight of the agent. Other preferred organic solvents are organic amines and alkanolamines. The agents according to the invention preferably contain these amines in amounts of 0.1 to 10% by weight, preferably 0.2 to 8.0% by weight, and more preferably 0.5 to 5.0% by weight, in each case based on their total weight. A particularly preferred alkanolamine is ethanolamine.

[0125] Detergents and / or cleaning agents according to the invention can contain exclusively the enzymes according to the invention. Alternatively, they can also contain further hydrolytic enzymes or other enzymes in a concentration appropriate for the effectiveness of the agent. A further embodiment of the invention thus represents agents which further comprise one or more further enzymes. Preferably used further enzymes are all enzymes which can exhibit catalytic activity in the agent according to the invention, in particular a protease, lipase, amylase, cellulase, hemicellulase, mannanase, tannase, xylanase, xanthanase, xyloglucanase, ß-glucosidase, pectinase, carrageenase, perhydrolase, oxidase, oxidoreductase, and mixtures thereof. Further enzymes are advantageously present in the agent in an amount of 1 x 10 -8 to 5 percent by weight based on active protein. Increasingly preferably, each additional enzyme is present in an amount of 1 x 10 -7The enzymes are contained in the inventive agents in amounts of from 0.00001 to 1 wt.%, from 0.00005 to 0.5 wt.%, from 0.0001 to 0.1 wt.%, and particularly preferably from 0.0001 to 0.05 wt.%, based on active protein. Particularly preferably, the enzymes exhibit synergistic cleaning performance against certain soils or stains, i.e., the enzymes contained in the agent composition support each other in their cleaning performance.

[0126] Examples of proteases are subtilisins BPN' from Bacillus amyloliquefaciens and Carlsberg from Bacillus licheniformis, protease PB92, subtilisins 147 and 309, the protease from Bacillus lentus, subtilisin DY, and the enzymes thermitase, proteinase K, and proteases TW3 and TW7, which are classified as subtilases but no longer as subtilisins in the narrower sense. Subtilisin Carlsberg is available in a further developed form under the trade name Alcalase® from Novozymes. Subtilisins 147 and 309 are marketed by Novozymes under the trade names Esperase® and Savinase®, respectively. Protease variants are derived from the protease from Bacillus lentus DSM 5483, described in e.g. WO 95 / 23221, WO 92 / 21760, WO 2013 / 060621 and EP 3660151. Other useful proteases are e.g.the enzymes available under the trade names Durazym®, Relase®, Everlase®, Nafizym®, Natalase®, Kannase®, Progress Uno 101 L® and Ovozyme® from Novozymes, the enzymes available under the trade names Purafect®, Purafect® OxP, Purafect® Prime, Excellase®, Properase®, Preferenz P100® and Preferenz P300® from Danisco / DuPont, the enzyme available under the trade name Lavergy pro 104 LS® from BASF, the enzyme available under the trade name Protosol® from Advanced Biochemicals Ltd., the enzyme available under the trade name Wuxi® from Wuxi Snyder Bioproducts Ltd., the enzyme available under the trade names Proleather® and Protease P® from Amano Pharmaceuticals Ltd., and the enzyme available under the name Proteinase K-16 from Kao Corp. The proteases from Bacillus gibsonii and Bacillus pumilus, which are disclosed in WO 2008 / 086916, WO 2007 / 131656, WO 2017 / 215925, WO 2021 / 175696 and WO 2021 / 175697, are also particularly preferably used.

[0127] Examples of amylases include the α-amylases from Bacillus licheniformis, Bacillus amyloliquefaciens, or Bacillus stearothermophilus, as well as their improved versions for use in detergents and / or cleaning agents. The enzyme from Bacillus licheniformis is available from Novozymes under the name Termamyl® and from Danisco / DuPont under the name Purastar® ST. Further developments of this α-amylase are available under the trade names Duramyl® and Termamyl® ultra (both from Novozymes), Purastar® OxAm (Danisco / DuPont), and Keistase® (Daiwa Seiko Inc.). The α-amylase from Bacillus amyloliquefaciens is marketed by Novozymes under the name BAN®, and derivatives of the α-amylase from Bacillus stearothermophilus are marketed under the names BSG® and Novamyl®, also by Novozymes. Furthermore, the α-amylase from Bacillus sp. is available for this purpose.A 7-7 (DSM 12368) and cyclodextrin glucanotransferase (CGTase) from Bacillus agaradherens (DSM 9948) are particularly suitable. Fusion products of all of these molecules can also be used. Furthermore, the further developments of α-amylase from Aspergillus niger and A. oryzae, available under the trade name Fungamyl® from Novozymes, are also suitable. Other commercial products that can be used advantageously include Amylase-LT® and Stainzyme® or Stainzyme® ultra or Stainzyme® plus, as well as Amplify™ 12L or Amplify Prime™ 100L, the latter also from Novozymes, and the PREFERENZ S® series from Danisco / DuPont, including PREFERENZ S100®, PREFERENZ S1000®, and PREFERENZ S210®. Variants of these enzymes obtainable by point mutations can also be used according to the invention.

[0128] Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein-engineered mutants are also included. Suitable cellulases are cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, and Acremonium, e.g., the fungal cellulases from Humicola insolens, Myceliophthora thermophila, and Fusarium oxysporum, disclosed in US 4,435,307, US 5,648,263, US 5,691,178, US 5,776,757, and WO 89 / 09259. Particularly suitable cellulases are alkaline or neutral cellulases with color-care properties. Examples of such cellulases are cellulases described in EP 0495257, EP 0531372, WO 96 / 11262, WO 96 / 29397, WO 98 / 08940. Other examples are cellulase variants as described in WO 94 / 07998, EP 0531315, EP 3212777, EP 3502243, EP 3653705, EP 3653706, US 5457046, US 5686593, US 5763254, WO 95 / 24471, WO 98 / 12307 and WO 99 / 01544 and WO 2019 / 122520.Examples of cellulases with endo-1,4-glucanase activity (EC 3.2.1.4) are described in WO 2002 / 099091, e.g., those having a sequence of at least 97% identity to the amino acid sequence of positions 1 to 773 of SEQ ID NO:2 of WO 2002 / 099091. Another example may comprise a GH44 xyloglucanase, e.g., a xyloglucanase enzyme having a sequence of at least 60% identity to positions 40 to 559 of SEQ ID NO:2 of WO 2001 / 062903. Other examples of cellulases include the GH45 cellulases described in WO 96 / 29397. Commercially available cellulases include Celluzyme™, Carezyme™, Carezyme Premium™, Celluclean™ (e.g. Celluclean™ 5000L and Cellulclean™ 4000T), Celluclean Classic™, Cellusoft™, Endolase®, Renozyme® and Whitezyme™ (Novozymes A / S), Clazinase™ and Puradax HA™ (Genencor International Inc.), KAC-500(B)™ (Kao Corporation), Revitalenz™ 1000, Revitalenz™ 2000 and Revitalenz™ 3000 (DuPont), as well as Ecostone® and Biotouch® (AB Enzymes).

[0129] Suitable lipases are, for example, from Thermomyces, e.g. from T. lanuginosus (formerly Humicola lanuginosa), as described in EP 0258068 and EP 0305216, lipase from strains of Pseudomonas (some of them now renamed Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes, P. cepacia, P. sp. strain SD705, P. wisconsinensis, Streptomyces lipases of the GDSL type, lipase from Thermobifida fusca, lipase from Geobacillus stearothermophilus, lipase from Bacillus subtilis and lipase from Streptomyces griseus and S. pristinaespiralis. Preferred lipases include, for example, the lipases originally obtainable from Humicola lanuginosa (Thermomyces lanuginosus) or further developed therefrom, in particular those with one or more of the following amino acid substitutions starting from the said lipase in the positions D96L, T213R and / or N233R, particularly preferably T213R and N233R.Preferred commercial lipase products include Lipolase™, Lipex™, Lipolex™ and Lipoclean™ (Novozymes A / S), Lumafast (Genencor / DuPont) and Lipomax (Gist-Brocades).

[0130] Suitable mannanases are, for example, the Bacillus subtilis endo-ß-mannanase, Bacillus sp. I633 Endo-ß-mannanase, Bacillus sp. AAI12 Endo-ß-mannanase, Bacillus sp. AA349 Endo-ß-mannanase, Bacillus agaradhaerens NCIMB 40482 Endo-ß-mannanase, Bacillus halodurans Endo-ß-mannanase, Bacillus clausii Endo-ß-mannanase, Bacillus licheniformis Endo-ß-mannanase, Humicola insolens Endo-ß-mannanase and Caldocellulosiruptor sp. Endo-ß-mannanase (cf. e.g. US 6060299, WO 99 / 64573, US 6566114 and WO 99 / 64619).

[0131] Pectate lyases suitable for detergents and / or cleaning agents are described, for example, in WO 2003 / 095638 or WO 2015 / 121133. Examples of suitable pectinolytic enzymes are also the enzymes and enzyme preparations available under the trade names Gamanase®, Pektinex AR®, X-Pect®, or Pectaway® from Novozymes, under the trade names Rohapect UF®, Rohapect TPL®, Rohapect PTE100®, Rohapect MPE®, Rohapect MA plus HC, Rohapect DA12L®, Rohapect 10L®, Rohapect B1 L® from AB Enzymes, and under the trade name Pyrolase® from Diversa Corp.

[0132] In the cleaning agents described herein, the enzymes to be used can also be formulated together with accompanying substances, such as those from fermentation. In liquid formulations, the enzymes are preferably used as liquid enzyme formulation(s).

[0133] The enzymes are generally not provided in the form of pure protein, but rather in the form of stabilized, storable, and transportable preparations. These prefabricated preparations include, for example, solid preparations obtained by granulation, extrusion, or lyophilization or, particularly in the case of liquid or gel-like products, solutions of the enzymes, preferably as concentrated as possible, with little water content, and / or containing stabilizers or other additives.

[0134] Alternatively, the enzymes can be encapsulated for both solid and liquid dosage forms, for example by spray-drying or extrusion of the enzyme solution together with a preferably natural polymer, or in the form of capsules, for example those in which the enzymes are enclosed as if in a solidified gel, or in those of the core-shell type, in which an enzyme-containing core is coated with a water-, air-, and / or chemical-impermeable protective layer. Additional active ingredients, such as stabilizers, emulsifiers, pigments, bleaching agents, or dyes, can be applied in superimposed layers. Such capsules are applied using methods known per se, for example by shake or roll granulation or in fluid-bed processes. Such granules, for example by applying polymeric film formers, are advantageously low in dust and, due to the coating, are stable during storage.

[0135] Furthermore, it is possible to package two or more enzymes together so that a single granulate has multiple enzyme activities.

[0136] The enzymes can also be incorporated into water-soluble films, such as those used, for example, in the packaging of unit-dose detergents and cleaning agents. Such a film allows the enzymes to be released upon contact with water. As used herein, "water-soluble" refers to a film structure that is preferably completely water-soluble. Preferably, such a film consists of (fully or partially hydrolyzed) polyvinyl alcohol (PVA).

[0137] The agents according to the invention may comprise one or more reversible enzyme inhibitors / stabilizers. The agents according to the invention may contain the reversible enzyme inhibitors / stabilizers in a concentration of 0.1 to 2 wt.%, preferably 0.3 to 1.5 wt.%, based on the total weight of the agent. If multiple inhibitors / stabilizers are present, these values ​​refer to the total concentration.

[0138] These can in particular be selected from the group consisting of polyols, such as glycerol or 1,2-ethylene glycol, benzamidine hydrochloride, borax, boric acids, boronic acids or their salts or esters or derivatives, in particular phenylboronic acid derivatives or 4-formylphenylboronic acid (4-FPBA), antioxidants, special peptide compounds and combinations thereof.

[0139] For the purposes of the present invention, "phenylboronic acid derivative" refers to a compound of formula (III). The compound of formula (III) has the following structural formula: where R is hydrogen, a hydroxyl, a C1-C6 alkyl, a substituted C1-C6 alkyl, a C1-C6 alkenyl, or a substituted C1-C6 alkenyl group. Preferably, the R radical in the phenylboronic acid derivative is a C1-C6 alkyl group, and among these, it is more preferably -CH3, -CH3CH2, or -CH3CH2CH2. More preferably, the R radical in the phenylboronic acid derivative is hydrogen. The phenylboronic acid derivative is particularly preferably 4-formylphenylboronic acid (4-FPBA).

[0140] The inhibitor / stabilizer compound used can be boric acid.

[0141] In particularly preferred embodiments, the washing and / or cleaning agent according to the invention is substantially free of boron-containing compounds. "Substantially free of boron-containing compounds" in this context means that the agents according to the invention contain less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight, and particularly preferably less than 0.1% by weight, of boron-containing compounds, based on the total weight of the agent. In very particularly preferred embodiments, the agents according to the invention are free of boron-containing compounds, i.e., they contain, in particular, no boric acid and / or phenylboronic acid derivatives.

[0142] In various embodiments, the enzyme and the inhibitor / stabilizer compound can be pre-formulated in an enzyme composition. As can be seen from the previous explanations, the enzyme protein constitutes only a fraction of the total weight of conventional enzyme preparations. Preferably used enzyme preparations contain between 0.1 and 40 wt.%, preferably between 0.2 and 30 wt.%, particularly preferably between 0.4 and 20 wt.%, and in particular between 0.8 and 10 wt.% of the enzyme protein. In such compositions, the inhibitor / stabilizer compound can be present in an amount of 0.05 to 35 wt.%, preferably from 0.05 to 10 wt.%, based on the total weight in the enzyme composition. This enzyme composition, which is also a component of the present invention, can then be used in agents according to the invention, in amounts that lead to the above-specified final concentrations in the agent.

[0143] A further subject matter of the invention is an agent for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that the agent contains a dispersin, wherein the dispersin has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably to at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150,

[0144] 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189,

[0145] 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234,

[0146] 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272,

[0147] 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, and 323, has at least one amino acid substitution and / or deletion. This also includes agents for (semi-)automated washing or cleaning systems such as robot mops or wet vacuum cleaners.

[0148] A preferred embodiment relates to an agent for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that the agent contains a dispersin, wherein the dispersin has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably to at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150,

[0149] 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189,

[0150] 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234,

[0151] 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272,

[0152] 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, at least one amino acid substitution and / or deletion selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71 H, L71 N, L71 R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81 L, D81 R, D81S, D81T,D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111 E, D111 M, D111 N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R,E150V, E150W, E150Y, G151A, G151C, G151 D, G151 L, G151 N, G151 P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171 E, D171 K, D171 L, D171 M, D171Q, D171 R, D171V, D171W, D171Y, 11730, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181 D, S181 F, S181G, S181 N, S181 P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S,D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251 L, S251 N, S251 R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261 D, E261 R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271 D, A271G, H272D, H272I, Y281 P, Y282E, Y282N, Y281 R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P,F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281 K, Y281 N, Y281 P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291 L, K291 R, K291V, T296C, E300A, E300D, H301C, H301 N, H301 R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R, and combinations thereof. This also includes agents for (semi-)automated washing or cleaning systems such as robot mops or wet vacuum cleaners.

[0153] Another subject matter of the invention relates to a method for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that in at least one method step a dispersin according to the invention, as described herein, and / or an agent according to the invention, as described herein, is used, wherein the method is preferably carried out in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

[0154] This includes both manual and mechanical processes, with mechanical processes being preferred due to their more precise controllability, for example, with regard to the quantities used and contact times. Processes for cleaning textiles are generally characterized by the application of various cleaning-active substances to the items to be cleaned in several process steps and the washing off after the contact time, or by the cleaning items being treated in some other way with a detergent or a solution or dilution of this detergent. This also includes processes using semi-automated washing or cleaning systems such as robotic mops or wet vacuum cleaners.

[0155] This subject matter of the invention also encompasses a machine dishwashing process. In such a process, the agent according to the invention can be dosed into the cleaning solution, for example, by means of the dosing chamber in the door or by means of an additional dosing container in the interior of the dishwasher. Alternatively, the agent can also be applied directly to the soiled dishes or to one of the interior walls of the dishwasher, for example the inside of the door. The process according to the invention is carried out in the interior of a commercially available dishwasher. In a dishwasher, the cleaning program can generally be selected and set by the consumer before the dishwashing process is carried out. The cleaning program of the dishwasher used in the process according to the invention comprises at least one pre-wash cycle and one cleaning cycle.According to the invention, cleaning programs are preferred which comprise further cleaning or rinsing cycles, e.g. a final rinse cycle. The method according to the invention is particularly preferably a component of a cleaning program comprising a pre-rinse cycle, a cleaning cycle and a final rinse cycle. The method according to the invention is preferably used in conjunction with cleaning programs in which the wash liquor is heated during the cleaning cycle. In a preferred embodiment of the method according to the invention, the cleaning cycle, during which the agent according to the invention is metered into the interior of the dishwasher, is characterized in that during its course the temperature of the cleaning liquor rises to values ​​above 30°C, preferably above 40°C and in particular above 50°C.

[0156] Alternative embodiments of this subject matter of the invention also include processes for treating textile raw materials or for textile care, in which an agent according to the invention is active in at least one process step. Among these, processes for textile raw materials, fibers, or textiles with natural components are preferred, and especially for those containing wool or silk.

[0157] All facts, objects, and embodiments described for the agent according to the invention are also applicable to these subject matters of the invention. Therefore, express reference is made here to the disclosure at the appropriate point, with the note that this disclosure also applies to the above-mentioned use according to the invention.

[0158] EXAMPLES

[0159] Example 1: Determination of bad odor and bioburden formation

[0160] Table 1 : Detergent matrices used

[0161] Before the start of the test, the household washing machine used (Miele Novotronic W1514) was assessed both sensorially and visually for the formation of deposits / biodeposits on the pump sump of the washing machine (- = no biodeposit, + = light biodeposit, ++ = heavy biodeposit). In preparation, approximately 2.0 kg of white textiles (T-shirts, terry towels) were cleaned and disinfected by repeated washing with a bleach-containing, commercially available heavy-duty detergent in the intensive program (95°C, 16°dH, 1400 rpm, Miele Novotronic W1514)) and dried in a tumble dryer. By repeatedly washing (30°C, 16°dH, 1400 rpm, 120 min) and storing the prepared laundry under standardized conditions in the prepared household washing machine, typical household washing conditions were simulated, thereby eliciting the corresponding deposit / biodeposit formation in the washing machine.Between wash cycles, the laundry was stored outside the washing machine, and the washing machine was incubated with the door closed. After the last wash cycle, the washing machine (i.e., the open washing drum) was sensorially assessed by a mixed panel of experts and consumers (malodor scale 0-3, 0 = no malodor, 3 = strong malodor; difference > 0.5 = significant). In addition to the sensory assessment, the formation of biodegradation on the washing machine's pump sump was also visually assessed (- = no biodegradation, + = slight biodegradation, ++ = strong biodegradation).

[0162] Table 2: Bad smell in the washing machine

[0163] * Dispersin according to the invention: SEQ ID NO:1 + Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-

[0164] N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q

[0165] Table 3: Biobelaq formation in the pump sump Dispersin according to the invention: SEQ ID NO:1 + Q3I-H15Y-A49W-N59E-S163P-S186R-S225G-

[0166] N227T-E232D-G235W-N252P-N260Q-H272V-S279D-Y281 P-K308Q-K309E-K312Q

[0167] The results show that the dispersin according to the invention not only reduces or prevents the unpleasant odor in the washing machine, but also not only reduces or prevents the formation of biodeposits, but even removes existing biodeposits. Example 2: Example formulations

[0168] The dispersin according to the invention can be used in various washing and cleaning agents and achieve its effect.

[0169] Table 4: Liquid detergents

[0170] Table 5: Liquid detergents

[0171]

[0172] Table 6: Solid detergents Table 7: Hand dishwashing detergent

[0173] Table 8: Two-phase dishwashing detergent

[0174]

Claims

PATENT CLAIMS 1 . Use of a dispersin having ß-1 ,6-N-acetylglucosaminidase activity, a Amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to the amino acid sequence given in SEQ ID NO:1 and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271 , 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, has at least one amino acid substitution and / or deletion, for preventing and / or reducing bad odors on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or -pipes, water inlets and / or outlets, and / or for preventing and / or reducing and / or removing microbial deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines,Water inlets and / or outlets, and / or to improve the hygiene of surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage tanks, water reservoirs, washing drums, rinsing chambers, washbasins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets.

2. Use according to claim 1, wherein the at least one amino acid substitution and / or deletion is selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71 H, L71 N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81 L, D81 R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111 E, D111 M, D111 N, D111Q, D111 R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R,D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151 D, G151 L, G151 N, G151 P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171 E, D171 K, D171 L, D171 M, D171Q, D171 R, D171V, D171W, D171Y, 11730, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181 D, S181 F, S181G, S181 N, S181 P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C,S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M, A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251 L, S251 N, S251 R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261 D, E261 R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y,M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271 D, A271G, H272D, H272I, Y281 P, Y282E, Y282N, Y281 R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281 H, Y281 K, Y281 N, Y281 P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291 L, K291 R, K291V, T296C, E300A, E300D, H301C, H301 N, H301 R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R and combinations thereof.

3. Use of a dispersin according to claim 1 or 2 for preventing and / or reducing bad odors on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets.

4. Use of a dispersin according to claim 1 or 2 for preventing and / or reducing and / or removing microbially caused deposits on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets.

5. Use of a dispersin according to claim 1 or 2 for improving hygiene on surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, WC rims, siphons, water pipes and / or lines, water inlets and / or outlets.

6. Use according to one of the preceding claims, wherein the dispersin is contained in a washing and / or cleaning agent, wherein the agent is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the agent, in a 1 wt. % solution in deionized water at 20°C, has a pH in a range from about 6 to about 11, in particular from about 6.5 to about 10.5, more preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

7. Use according to one of the preceding claims, preferably in a temperature range of about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

8. Agents for cleaning surfaces, especially household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, Washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that the agent contains a dispersin, wherein the dispersin has ß-1,6-N-acetylglucosaminidase activity, comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably to at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical and, based on the numbering according to SEQ ID NO:1, at least one of the positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32,34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71 , 72, 74, 77, 79, 80, 81 , 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111 , 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131 , 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151 , 152, 163, 164, 167, 168, 170, 171 , 173, 174, 175, 177, 178, 179, 181 , 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221 , 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251 , 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, has at least one amino acid substitution and / or deletion.

9. Agent according to claim 8, wherein the at least one amino acid substitution and / or deletion is selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71 N, L71 R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81 L, D81 R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N,V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111 E, D111 M, D111 N, D111Q, D111 R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V, T120W, D122*, D122H, D122R, Y123W, Y124C, Y124I, Y124K, Y124L, Y124M, Y124Q, Y124R, Y124T, Y124V, Y124W, D125C, D125G, D125K, D125Q, D125R, N126V, R127D, R127H, R127K, R127L, R127M, R127Q, R127W, V128C, V128L, V128T, D131V, Q135*, Q135A, Q135D, Q135E, Q135K, Q135M, Q135Y, D138K, D138L, D138M, D138Q, D138R, D138S, D138V, D138W, E139W, D142R, D142W, Y145*, Y145H, Y145L, Y145N, Y145V, P147A, P147C, P147D, P147F, P147G, P147L, P147M, P147R, P147S, P147T, P147V, K148A, K148D, K148L, K148V, F149L, F149M, F149N, E150D, E150H, E150K, E150L, E150M, E150N, E150R, E150V, E150W, E150Y, G151A, G151C, G151 D, G151 L, G151 N, G151 P, G151S, G151W, K152D, K152L, K152R, G164D, G164E, G164H, G164S, G164V, V167D, V167E, V167L, V167P, V167Q, V167R, V167W, H168N, L170A, L170D, L170E, L170F, L170H, L170K, L170M, L170N, L170P, L170Q, L170R, L170S, L170V, L170W, L170Y, D171A, D171C, D171 E, D171 K, D171 L, D171 M, D171Q, D171 R, D171V, D171W, D171Y, 11730, D174H, D174M, D174N, D174V, D174W, F175Y, N177M, Q178*, Q178A, Q178K, Q178R, Q178W, I179T, S181C, S181 D, S181 F, S181G, S181 N, S181 P, S181Q, S181T, S181V, S181W, E185M, E185R, E185V, E185W, S186D, S186E, S186H, S186I, S186K, S186L, S186M, S186N, S186Q, S186R, S186V, S186W, K187C, K187D, K187G, K187R, K187S, K187V, K187W, Y188P, E189L, E189V, E189W, S199C, S199L, S199M, S199Y, E200D, E200F, E200K, E200L, E200M, E200N, E200R, E200W, A203C, A203D, A203E, A203G, A203L, A203M,A203P, A203R, A203S, A203T, A203V, A203W, N204L, N204M, N204V, N204W, N204Y, L205I, D207A, D207C, D207E, D207G, D207N, D207Q, D207S, D207V, D207W, S208A, S208C, S208D, S208G, S208L, S208Q, S208T, S208V, S208W, S210T, Q215R, Q215M, Q215L, Q215*, S217V, T218A, T218L, T218Q, T218R, T218V, G222D, E224A, E224P, N227A, N227Q, N227R, N227S, N227T, N227K, D230*, D230R, D230T, D230W, E232D, E232V, N233H, N233Q, N233R, N233W, W234R, G235W, G235A, G235E, G235F, G235H, G235I, G235L, G235M, G235N, G235P, G235S, G235V, S237C, S237G, S237M, S237N, S237W, S237Y, Y244C, Y244E, Y244M, L249H, L249K, L249Q, L249R, L249W, L249Y, S251 L, S251 N, S251 R, S251W, N252P, N252C, G253D, G253W, F254I, F254L, F254M, F254Y, Q256E, Q256R, N260*, N260A, N260C, N260E, N260I, N260K, N260L, N260M, N260Q, N260R, N260V, N260W, N260Y, E261*, E261A, E261 D, E261 R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271 D, A271G, H272D,H272I, Y281 P, Y282E, Y282N, Y281 R, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281 H, Y281 K, Y281 N, Y281 P, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291 L, K291 R, K291V, T296C, E300A, E300D, H301C, H301 N, H301 R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, N323R and combinations thereof.

10. A composition according to claim 8 or 9, wherein the composition is substantially free of boron-containing compounds, preferably free of boron-containing compounds; and / or the composition, in a 1 wt.% solution in deionized water at 20°C, has a pH in a range from about 6 to about 11, in particular from about 6.5 to about 10.5, more preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

11. A method for cleaning surfaces, in particular household surfaces, preferably in washing machines or dishwashers, pump sumps, storage containers, water reservoirs, washing drums, rinsing chambers, wash basins or sinks, toilet bowls, cisterns, toilet rims, siphons, water pipes and / or lines, water inlets and / or outlets, in particular for preventing and / or reducing bad odors and / or for preventing and / or reducing and / or removing microbially caused deposits and / or for improving hygiene, characterized in that in at least one method step a dispersin according to claim 1 or 2 or an agent according to one of claims 8 to 10 is used, the method preferably being carried out in a temperature range from about 10°C to about 60°C, preferably about 15°C to about 40°C, particularly preferably about 20°C to about 30°C.

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