Kit and method for filling a pipette with a liquid

Abstract

Kit for filling a pipette (1) with a liquid (L), the kit comprising: a tip (20) defining a channel (27) for a liquid (L), the channel (27) comprising an inlet orifice (30) having an inlet diameter (D) and an outlet orifice (32) having an outlet diameter (d) smaller than the inlet diameter, the tip (20) being suitable for being connected to the pipette (1) so as to fluidly connect the outlet orifice (32) with a supply orifice (12) of the pipette (1); and a centrifuge (40) suitable for centrifuging the pipette (1) and the tip (20) connected to the pipette (1), so as to cause a displacement of a liquid (L) deposited in the channel (27) towards a distal end (4) of the pipette (1) opposite the supply orifice (12). Figure for abstract: Fig. 1

Description

Title of the invention: Kit and method for filling a pipette with a liquid Technical field

[0001] The present disclosure relates to a kit for filling a pipette with a liquid, and a method of filling a pipette with a liquid using such a kit. STATE OF THE ART

[0002] A pipette has a proximal end provided with a feed orifice for filling the pipette with a liquid. However, when the feed orifice is very small, filling the pipette can be difficult. Furthermore, in some applications, it is desired to place the liquid at the distal end of a pipette. However, such placement can be difficult when the liquid is viscous.

[0003] One possible solution for filling a pipette with such constraints is to use a syringe equipped with a flexible capillary which is inserted into the pipette in order to bring the ink to the distal end of the pipette. However, this filling step is delicate and requires a certain amount of know-how, especially when filling the finest pipettes. In addition, the flexible capillaries used as consumables have the disadvantage of being expensive. SUMMARY

[0004] An aim of the present disclosure is to propose a solution making it possible to fill a pipette more easily and inexpensively in the long term.

[0005] This object is achieved by a filling kit comprising a tip and a centrifuge. The tip defines a channel for a liquid, the channel comprising an inlet orifice having an inlet diameter and an outlet orifice having an outlet diameter smaller than the inlet diameter, the tip being adapted to be connected to the pipette so as to fluidly connect the outlet orifice with a supply orifice of the pipette. The centrifuge is adapted to centrifuge the pipette and the tip connected to the pipette, so as to cause a displacement of a liquid deposited in the channel towards a distal end of the pipette opposite the supply orifice.

[0006] Since the diameter of the inlet orifice of the tip is larger than the diameter of the outlet orifice, it is easier to get the liquid into the tip via this inlet orifice. Moving the liquid from the tip to the distal end of the pipette is no more difficult, since this step is performed by the centrifuge. Furthermore, the tip can be reused several times, and does not necessarily have to be flexible. Under these conditions, the tip can be much less expensive than a set of single-use flexible capillaries. After a certain number of of uses, the cost of the centrifuge can be amortized by the savings made on flexible capillaries. This is why the proposed kit allows for savings in the long term.

[0007] The filling kit may also include the following features, taken alone or combined with each other whenever technically possible.

[0008] Preferably, the inlet orifice and the outlet orifice are oriented relative to each other to allow an inlet of a liquid into the nozzle via the inlet orifice in a first direction, and an outlet of a liquid from the nozzle via the outlet orifice in a second direction different from the first direction.

[0009] Preferably, the second direction is perpendicular to the first direction.

[0010] Preferably, the channel comprises: a first channel portion extending in the first direction and terminating in the inlet orifice, a second channel portion extending in the second direction and terminating in the outlet orifice, and an elbow connecting the first channel portion to the second channel portion.

[0011] Preferably, the outlet diameter is between 1 millimeter and 1.2 millimeters.

[0012] Preferably, the tip has a surface delimiting the channel, flared towards the outlet orifice and suitable for serving as a stop for a proximal end of the pipette, when the proximal end of the pipette is inserted into the channel via the outlet orifice.

[0013] Preferably, the flared surface has a circular section.

[0014] Preferably, the tip comprises two jaws suitable for clamping the pipette when the proximal end of the pipette is inserted into the channel via the outlet orifice.

[0015] Preferably, the centrifuge comprises: a housing, a support rotatable relative to the housing, and a clip for attaching the pipette to the support, the clip comprising a foam to prevent by friction a centrifugal movement of the pipette relative to the support when the support is rotating relative to the housing.

[0016] A second object proposed in the present disclosure is a method of filling a pipette with a liquid using the kit discussed above. The method comprises: connecting the tip to the pipette such that the outlet port is fluidly connected with the supply port of the pipette, and centrifuging using the centrifuge the pipette and the tip connected to the pipette, such that a liquid deposited in the channel through the inlet port moves to the distal end of the pipette.

[0017] Preferably, the method comprises depositing the liquid into the channel via the inlet port after the tip has been connected to the pipette.

[0018] Preferably, the method comprises positioning the pipette and tip in the centrifuge after the tip has been connected to the pipette.

[0019] Preferably, the liquid is an ink.

[0020] A third object proposed in the present disclosure is a method of depositing a liquid on a substrate. This method comprises: filling a pipette with a liquid by carrying out the method constituting the second object of the present disclosure, placing an ejection orifice formed at the distal end of the pipette facing the substrate, and oscillating the pipette using a mechanical resonator, so as to cause ejection of the liquid from the pipette via the ejection orifice. DESCRIPTION OF FIGURES

[0021] Other characteristics, aims and advantages of the invention will emerge from the following description, which is purely illustrative and non-limiting, and which must be read in conjunction with the appended drawings in which:

[0022] [Fig.l] is a schematic sectional view of a pipette.

[0023] [Fig.2] is a top view of a tip according to one embodiment.

[0024] [Fig.3] is a sectional view of the tip shown in [Fig.2].

[0025] [Fig.4] is a perspective view of a centrifuge according to a method of realization, in a closed position.

[0026] [Fig.5] is a perspective view of the centrifuge shown in [Fig.4], in an open position.

[0027] [Fig.6] is a sectional view of the centrifuge shown in [Fig.5].

[0028] [Fig.7] shows the steps of a method of filling a pipette with a liquid, according to one embodiment.

[0029] Throughout the figures, similar elements bear identical references. DETAILED DESCRIPTION OF THE INVENTION

[0030] Referring to [Fig.l], a pipette 1 is elongated along a longitudinal axis XI from a proximal end 2 to a distal end 4.

[0031] The pipette 1 comprises a wall 6 extending around the longitudinal axis. The wall 6 has an internal surface closed on itself, delimiting an elongated cavity 9 of the pipette 1 for storing a liquid extending along the longitudinal axis XI, and an external surface 10 opposite the internal surface 8.

[0032] The cavity 9 ends with a supply orifice 12 opening at the proximal end of the pipette 1. This supply orifice 12 serves to supply the pipette 1 with liquid, in the sense that a liquid can be stored in the cavity of the pipette 1 by passing through this supply orifice 12.

[0033] The cavity 9 also ends with an injection orifice 14 opening at the distal end 4 of the pipette 1. As will be seen later, it is through this injection orifice 9 that a liquid stored in the pipette 1 is intended to be ejected from the pipette 1.

[0034] The feed orifice 12 and the ejection orifice 14 are coaxial. Thus, a liquid enters the pipette 1 via the feed orifice by flowing parallel to the longitudinal axis XI, and a liquid present in the cavity 9 leaves the pipette 1 via the ejection orifice by flowing parallel to the longitudinal axis XL.

[0035] The distal end 4 is tapered (in the literature, it is also referred to as a tip). The ejection orifice 14 is located at the end of the tip.

[0036] The proximal end 2 is tubular (its external surface is cylindrical in revolution).

[0037] The feed orifice has a feed diameter, and the ejection orifice has an ejection diameter smaller than the feed diameter. A liquid can remain trapped in the cavity of the pipette 1 by capillary effect, even if the ejection orifice 14 is oriented downwards.

[0038] The feed diameter is preferably between 100 and 1000 micrometers.

[0039] The ejection diameter is preferably between 50 micrometers and 100 nanometers.

[0040] The pipette is for example made of glass. This glass is preferably a quartz glass (called in English “fused quartz”), which is a more mechanically resistant glass.

[0041] With reference to [Fig.2] and [Fig.3], a tip 20 according to one embodiment is elongated along a longitudinal axis from a proximal end 22 to a distal end 24 opposite the proximal end 22.

[0042] The tip 20 has an internal surface 26 closed on itself, so as to delimit a channel 27 in which a liquid, for example an ink, can flow.

[0043] The tip also has an external surface 28 opposite the internal surface 26.

[0044] The external surface 28 notably comprises a distal surface forming the distal end, and a lateral surface which extends around the longitudinal axis X2.

[0045] The channel 27 comprises an inlet orifice 30 opening into the lateral surface, outside the end piece 20. The channel 27 further comprises an outlet orifice 32. Thus, a liquid can enter the end piece 20 via the inlet orifice 30, then exit the end piece 20 via the outlet orifice 32.

[0046] The channel 27 comprises a first channel portion 27a extending in a transverse direction perpendicular to the longitudinal axis X2, and terminating in the inlet orifice 30. The inlet orifice 30 is oriented so as to allow a liquid to penetrate into the nozzle 20 via the inlet orifice 30 and pass through the first by flowing in the transverse direction.

[0047] The channel 27 further comprises a second channel portion 27b extending in a longitudinal direction parallel to the longitudinal axis X, and ending in the outlet orifice 32. The outlet orifice 32 is oriented so as to allow a liquid to exit the nozzle 20 by flowing in the longitudinal direction.

[0048] The channel 27 further comprises an elbow connecting the first portion 27a to the second portion 27b. In this way, a liquid having entered the nozzle 20 via the inlet orifice 30 and then having flowed into the first portion makes a simple 90° turn at the elbow, before flowing into the second portion and then exiting the nozzle 20 via the outlet orifice 32. It will be noted in passing that if the nozzle 20 is oriented in space such that the transverse direction is vertical, then liquid can be trapped by capillarity in the nozzle 20 (the liquid will not be able to flow naturally into the second portion of the channel 27, which is then horizontal).

[0049] The tip 20 is suitable for being connected to the proximal end of the pipette 1 so that the outlet orifice 32 coincides with the supply orifice 2 of the pipette 1. Thus, if a liquid located in the tip 20 leaves the tip 20 via the outlet orifice 32, this liquid enters the cavity 9 of the pipette 1 via its supply orifice 2.

[0050] When the tip 20 and the pipette 1 are connected, the longitudinal axis XI of the pipette 1 and the longitudinal axis X2 of the tip 20 are merged. The second channel portion 27b and the elongated cavity of the pipette 1 are thus aligned.

[0051] The outlet orifice 32 has an outlet diameter d adapted to allow insertion of the proximal end of the pipette 1 into the tip 20, more precisely into the second channel portion 27b, via the outlet orifice 32. Thus, in this embodiment, the connection of the tip 20 with the pipette 1 is made by such an insertion. Once connected, the distal end 24 of the tip extends around the side wall 6 of the pipette, and the supply orifice 12 of the pipette 1 opens into the second channel portion 27. It is much easier for a user to make a connection by such an insertion, rather than inserting the tip 6 or a tube into the pipette 1, which has a very small supply diameter.

[0052] The outlet orifice 32 is circular.

[0053] Preferably, the outlet orifice 32 has an outlet diameter d between 1 millimeter and 2.2 millimeters, or even between 1 millimeter and 1.2 millimeters or between 2 millimeters and 2.2 millimeters. Due to the orientation of the outlet orifice 32, the outlet diameter d is measured in a transverse plane perpendicular to the axis X2.

[0054] The internal surface 26 of the end piece 20 comprises a flared surface 34 extending around the longitudinal axis X2. This flared surface 34 delimits the outlet orifice 32. This surface is flared towards the outlet orifice 32, that is to say that the area of ​​its section increases as one approaches the outlet orifice 32 along the longitudinal axis X2. The flared surface 34 is suitable for serving as a stop for the proximal end 2 of the pipette 1, when the proximal end 2 of the pipette 1 is inserted into the channel 27 via the outlet orifice 32. The flared nature of this surface makes it possible to ensure this stop function for pipettes 1 having different respective diameters.

[0055] Preferably, the flared surface 34 is of revolution. This allows the proximal end 2 of the pipette 1 to bear on the flared surface 34 in a sealed manner when the pipette 1 is also of revolution (the contact between the proximal end 2 and the flared surface 34 is then made on a circular line). Consequently, a liquid flowing in the second portion of the channel 27 towards the pipette does not risk leaking outside the “pipette 1 + tip 20” assembly via a gap which would remain between the external surface 10 of the pipette 1 and the flared surface 34.

[0056] The inlet orifice 30 has an inlet diameter D greater than the outlet diameter d. Due to the orientation of the inlet orifice 30, the inlet diameter D is measured in a longitudinal plane perpendicular to the transverse direction. Thanks to this characteristic, it is much easier to inject a liquid into the tip 20 than to directly inject a liquid into the pipette 1 without passing through the tip 20.

[0057] The inlet orifice 30 is trapezoidal in the embodiment illustrated in the figures. The inlet orifice 30 has a length, measured parallel to the longitudinal axis X2 (vertically in [Fig. 2]), which is between 3 and 4 millimeters. The inlet orifice further has a width, measured perpendicular to a plane defined by the longitudinal and transverse directions (horizontally in [Fig. 2]), which is between 3 and 5 millimeters.

[0058] The tip 20 further comprises two jaws 36, 38 extending parallel to the longitudinal axis X2 to the distal end 24 of the tip 20. The two jaws 36, 38 are opposite each other, so as to delimit between them a notch which extends between the outlet orifice 32 and the distal end 24. The notch opens onto two opposite sides of the external surface 28. When the tip 20 and the pipette 1 are connected, the pipette 1 extends into the notch, and the two jaws 36, 38 grip the pipette 1, so as to prevent, by friction, a movement of the pipette 1 relative to the tip 20 along the axis X2.

[0059] Preferably, the tip 20 is obtained by an additive manufacturing process. The tip may in particular be made of a photosensitive resin, which undergoes photo-crosslinking during additive manufacturing. Such a process is inexpensive and makes it possible to obtain varied shapes.

[0060] With reference to [Fig.4], [Fig.5] and [Fig.6], a centrifuge 40 according to one embodiment comprises a housing 42 delimiting a centrifugation cavity 43.

[0061] The housing 42 comprises a base 44 and a cover 46 movable between a closed position, so as to fluidically seal the cavity 41 from the outside of the centrifuge 40, and an open position, allowing access to the centrifugation cavity 41 from the outside of the centrifuge 40. The cover 46 is rotatably mounted on the base 44 by means of a hinge 48. Thus, the cover 46 passes from the closed position to the open position by rotation, and vice versa.

[0062] The housing 42 is intended to be placed in a position of use, in which the cover 46 is located above the base 44. In order that the housing 42 can be stabilized in this position of use, the base 44 may have feet (at least three feet) or equivalent stabilizing means.

[0063] The centrifuge 40 further comprises a support 50 movable in rotation relative to the housing 42, extending into the centrifugation cavity 4L.

[0064] The centrifuge further comprises a motor 52 (visible in [Fig.6]) for rotating the support 50. The motor 52 is for example a direct current motor, powered at 3V by two 1.5V batteries connected in series. A rotation speed of the support 50 achievable by the motor 52 is 5000 revolutions per minute.

[0065] The centrifuge also includes a control interface 54 for turning the motor 52 on and off. The control interface 54 may also be adapted to vary the rotational speed of the support 50 within a predefined speed range. In the illustrated embodiment, the control interface 54 includes a thumbwheel on the housing 42, allowing this rotational speed to be varied when a user turns the thumbwheel.

[0066] The support 50 has a support surface 56 perpendicular to its axis of rotation Z. The support surface 56 faces the cover 46 when the cover 46 is in its closed position. When the cover 46 is in its open position, the support surface 56 is visible from outside the centrifuge 40, and a user can then easily place the tip 20 and the pipette 1 on the upper surface.

[0067] When the housing 42 is in its position of use (cover 46 above the base 44), the axis of rotation Z is vertical, and the support surface 56 extends horizontally.

[0068] The support surface 56 delimits a compartment adapted to receive the tip 20 and at least the proximal end of the pipette 1 in a centrifugation position. The compartment has a radial opening suitable for being traversed by the pipette 1. Thus, while the tip 20 and the proximal end 2 of the pipette 1 are received in the compartment, the distal end 4 of the pipette can extend into the centrifugation cavity 41 outside the support 50.

[0069] In the centrifugation position, the tip 20 is connected to the proximal end of the pipette 1 so that the outlet orifice 32 coincides with the supply orifice 12 of the pipette 1, thus establishing a fluid connection between the channel 27 and the cavity 9. As indicated previously, when these two elements are connected, the proximal end 12 extends into the second channel portion 27, and the pipette is held clamped between the two jaws 36, 38.

[0070] In the centrifugation position, the tip 20 is located between the rotation axis Z and the distal end 4 of the pipette 1. Preferably, the longitudinal axis X2 of the tip 20 crosses the rotation axis Z of the support 50, that is to say that the “tip 20 + pipette 1” assembly is positioned radially.

[0071] The centrifuge 40 further comprises a clip for attaching the “tip 20 + pipette 1” assembly to the support 50. For example, the clip comprises a clasp rotatably mounted on the support 50 by a hinge between an open position and a closed position.

[0072] In its closed position, the clasp covers a portion of the pipette 1, so that the pipette 1 extends between the support surface 56 and the clasp. The fastener further comprises a foam fixed to the clasp, so as to come into contact with the pipette 1 when the fastener is in its closed position. The foam prevents by friction a centrifugal movement of the pipette 1 relative to the support 50, when the support 50 is rotating relative to the housing 42.

[0073] The support 50 may comprise more than one compartment and more than one attachment, so that it can serve as a support 50 for several “tip 20 + pipette 1” assemblies simultaneously. For example, the support 50 comprises a plurality of compartments distributed in balanced manners around the axis of rotation of the support 50, that is to say that the angular distance between two adjacent compartments may be constant. In the illustrated embodiment, the support 50 comprises two fins extending on either side of the axis of rotation Z on two fins, each fin defining a compartment.

[0074] The tip 20 and the centrifuge 40 form a kit for filling the pipette 1 with a liquid such as ink.

[0075] With reference to [Fig.7], a method of filling the pipette 1 using this kit comprises the following steps.

[0076] In a connection step E1, the tip 20 is connected to the pipette 1, as indicated previously. This connection can be carried out manually by a user.

[0077] In a positioning step E2, the tip 20 and the pipette 1 are positioned on the support 50 in the centrifugation position described previously. This step can also be carried out manually by a user. The attachment, shown in [Fig.7] by a black rectangle, is placed in its closed position, so as to prevent movement of the pipette 1 relative to the support 50, in particular centrifugal movement. It will be noted that the foam, retaining the pipette 1 by friction, has the advantage of not damaging or risking breaking the pipette 1. At this stage, the inlet orifice 30 of the tip is oriented upwards.

[0078] In a deposition step E3, a liquid L, for example an ink, is injected into the tip 20 via its inlet orifice 30. For example, a single drop of liquid of approximately 10 microliters is injected into the tip 20 via the inlet orifice 30. At this stage, the injected liquid L remains in the tip 20, at the bend of the channel 27 or in the second channel portion 27b, which is at this stage oriented horizontally. The arrangement of the inlet orifice 30 relative to the outlet orifice 32 makes it possible to greatly facilitate this deposition step E3 by a user. Furthermore, carrying out the deposition step E3 after the connection and positioning steps has the advantage of limiting the risks of handling likely to accidentally spill part of the liquid L to be injected.

[0079] In a centrifugation step E4, the pipette 1 and the tip 20 connected to the pipette 1 are centrifuged using the centrifuge 40. More precisely, this step comprises closing the cover 46, then rotating the support 50 relative to the housing 42 using the control interface 54. This rotation causes a displacement of the liquid L received in the channel 27 towards the distal end 4 of the pipette 1, under the effect of the centrifugal force generated.

[0080] For example, when the liquid L is an ink having a viscosity of 1000 cP, the diameter of the ejection orifice 14 is 5 μm, and when the support 50 rotates at 5000 revolutions per minute, 20 seconds of rotation may be sufficient to deliver the ink to the distal end of the pipette. The more viscous the liquid L and the smaller the ejection orifice, the higher the rotation time required. Conversely, the higher the rotation speed of the support 50, the shorter the rotation time of this support required. By thus adjusting the speed and rotation time of the support 50, it is then possible to fill any size of pipette with liquids (in particular inks) whose viscosity can vary from 1 to 100,000 cP.For example, in comparison, to fill a pipette with a feed diameter of 100 nanometers with a liquid having a surface tension of 30 mN / m, a pressure of about 3 bar must be applied, just above the maximum pressure of 2 bar that a thumb can apply.

[0081] Once the liquid L is near the distal end 4 of the pipette, as visible at the bottom of [Fig.7], the filling is considered complete.

[0082] This filling may constitute a preliminary step of a method for depositing the liquid L on a substrate. The subsequent steps of this method, described in document WO2020128310A1, are as follows.

[0083] The tip 20 is detached from the pipette 1, and the pipette 1 filled with the liquid L is extracted from the centrifuge 40.

[0084] Next, a user places the pipette 1 filled with the liquid L in a vertical use position, in which the pipette 1 is mechanically coupled with a mechanical resonator, and in which the ejection orifice 14 of the pipette 1 is oriented downwards opposite a substrate.

[0085] The mechanical resonator is started, which has the effect of causing the pipette 1 to oscillate in a controlled manner. This oscillation causes an ejection of the liquid L out of the pipette 1 via the ejection orifice 14 towards the substrate.

Claims

Claims

1. Kit for filling a pipette (1) with a liquid (L), the kit comprising: • a tip (20) defining a channel (27) for a liquid (L), the channel (27) comprising an inlet orifice (30) having an inlet diameter (D) and an outlet orifice (32) having an outlet diameter (d) smaller than the inlet diameter, the tip (20) being suitable for being connected to the pipette (1) so as to fluidically connect the outlet orifice (32) with a supply orifice (12) of the pipette (1), • a centrifuge (40) suitable for centrifuging the pipette (1) and the tip (20) connected to the pipette (1), so as to cause a displacement of a liquid (L) deposited in the channel (27) towards a distal end (4) of the pipette (1) opposite the supply orifice (12).

2. Kit according to the preceding claim, wherein the inlet orifice (30) and the outlet orifice (32) are oriented relative to each other to allow an inlet of a liquid (L) into the nozzle (20) via the inlet orifice (30) in a first direction, and an outlet of a liquid (L) from the nozzle (20) via the outlet orifice (32) in a second direction (X2) different from the first direction.

3. Kit according to the preceding claim, in which the second direction is perpendicular to the first direction.

4. Kit according to any one of claims 2 and 3, in which the channel (27) comprises: • a first channel portion (27a) extending in the first direction and ending in the inlet orifice (30), • a second channel portion (27b) extending in the second direction (X2) and ending in the outlet orifice (32), • an elbow connecting the first channel portion (27) to the second channel portion (27).

5. A kit according to any preceding claim, wherein the outlet diameter (d) is between 1 millimeter and 1.2 millimeters.

6. Kit according to any one of the preceding claims, in which the tip (20) has a surface (34) delimiting the channel (27), flared towards the outlet orifice (32) and suitable for serving as a stop for a proximal end (2) of the pipette (1), when the proximal end of the pipette (1) is inserted into the channel (27) via the outlet orifice (32).

7. Kit according to the preceding claim, in which the flared surface (34) has a circular section.

8. A kit according to any preceding claim, wherein the tip (20) comprises two jaws (36, 38) adapted to clamp the pipette (1) when the proximal end (2) of the pipette (1) is inserted into the channel (27) via the outlet orifice (32).

9. A kit according to any preceding claim, wherein the centrifuge (40) comprises: • a housing (42), • a support (50) rotatable relative to the housing (42), and • a clip for attaching the pipette (1) to the support (50), the clip comprising a foam to prevent centrifugal movement of the pipette (1) relative to the support (50) by friction when the support (50) is rotating relative to the housing (42).

10. A method of filling a pipette (1) with a liquid (L) using a kit according to any one of the preceding claims, the method comprising: • connecting the tip (20) to the pipette (1) so that the outlet orifice (32) is fluidly connected with the supply orifice (12) of the pipette (1), • centrifuging the pipette (1) using the centrifuge (40) and the tip (20) connected to the pipette (1), so that a liquid (L) deposited in the channel (27) through the inlet orifice (30) moves to the distal end of the pipette (1).

11. Method according to the preceding claim, comprising: • deposit the liquid (L) into the channel (27) via the inlet port (30) after the tip (20) has been connected to the pipette (1).

12. A method according to any one of claims 10 and 11, comprising: • positioning the pipette (1) and the tip (20) in the centrifuge (40) after the tip (20) has been connected to the pipette (1).

13. Method according to one of claims 10 to 12, in which the liquid (L) is an ink.

14. A method of depositing a liquid (L) on a substrate, the method comprising: • filling a pipette (1) with a liquid (L) by implementing the method according to any one of claims 10 to 13, • placing an ejection orifice (14) formed at the distal end (4) of the pipette (1) opposite the substrate, • oscillating the pipette (1) using a mechanical resonator, so as to cause ejection of the liquid (L) from the pipette (1) via the ejection orifice (14).

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