Surface Treatment

Abstract

A method for treating a surface comprising the step of treating the surface with bacterial spores and Saccharomyces supernatant.A product comprising bacterial spores and Saccharomyces supernatant.

Description

[Technical field]

[0001] The present invention relates to methods of treating surfaces to reduce and / or eliminate malodours during and after treatment.The present invention also relates to products that provide sustained malodour removal. [Background technology]

[0002] Cleaning and odor are closely related. The presence of malodor on a surface implies a lack of cleanliness. Malodor can be associated with a dirty surface or can occur during use on a previously cleaned surface. In the case of fabrics, malodor can occur when the fabric is damp or wet, for example after the washing process or in damp weather. Malodor can also occur during the wear of the fabric.

[0003] WO 2020 / 150587 A1 discloses a laundry composition comprising a detergent surfactant and a Saccharomyces fermentation filtrate. The laundry detergent composition provides benefits in eliminating, inhibiting, and / or reducing any malodor, such as moisture malodor, body malodor, or food malodor.

[0004] WO 2017 / 157771 A1 discloses a process for controlling malodour using bacterial spores that can inhibit or prevent the production of malodour. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2020 / 150587(A1) [Patent Document 2] International Publication No. 2017 / 157771(A1) Summary of the Invention [Problem to be solved by the invention]

[0006] While various methods have been provided for removing, inhibiting and / or reducing malodors, there remains a need to provide improved methods for removing, inhibiting, preventing and / or reducing malodors from surfaces during and after the cleaning process. [Means for solving the problem]

[0007] According to a first aspect of the present invention there is provided a method of treating a surface comprising: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; and The method includes a process of treating with.

[0008] According to a second aspect of the invention there is provided a product suitable for use in the method of the invention, the product comprising: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; and Includes.

[0009] The methods and products of the present invention can be applied to any surface, and can be applied to hard or soft surfaces. The methods and products of the present invention are particularly suited for treating fabrics in the laundry process.

[0010] According to a third aspect of the present invention there is provided the use of the method of the present invention to provide sustained malodour removal from fabrics over an extended period of time.

[0011] The elements of the composition of the invention described in relation to the first aspect of the invention apply mutatis mutandis to the other aspects of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The present invention includes a method of treating a surface. The surface may be a hard or soft surface, preferably the surface is a fabric. The method comprises treating the surface with a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; and The method includes the step of treating with.

[0013] The present invention also includes products for treating surfaces. The methods and products of the present invention provide malodor removal and prevention for a sustained period of time, not only during treatment, but also during use of the surface after it has been treated.

[0014] The method and product of the present invention have been unexpectedly found to provide a synergistic effect over a sustained period of time with respect to the removal and prevention of malodors. This synergistic effect has been surprisingly found over a sustained period of time. Without being bound by theory, it is believed that the benefits of the mixed system, i.e., bacterial spores and Saccharomyces supernatant, result from the production of a highly complex cocktail of enzymes from the combination of enzymes already present in the Saccharomyces supernatant and enzymes produced by the bacterial spores after they germinate and grow on the fabric. Because yeasts such as Saccharomyces cerevisiae are very different from bacteria, their enzymes have a very different structure than those produced by bacteria. The end result is a highly efficient system that can destroy odors much more than the two individual components.

[0015] The present invention also encompasses the use of the method of the present invention to provide sustained malodor removal from fabrics. By "sustained malodor removal" is meant that malodor removal occurs for at least 24 hours, preferably at least 48 hours, after the fabric has been treated. Without wishing to be bound by theory, it is believed that bacterial spores germinate with heat and sweat from the user, thereby providing malodor removal and prevention while the fabric is being worn.

[0016] As used herein, the articles "a" and "an" when used in a claim are understood to mean one or more of what is claimed or described. As used herein, the terms "include", "includes" and "including" are meant to be open-ended. The compositions of the present disclosure may comprise, consist essentially of, or consist of the components of the present disclosure.

[0017] All percentages, ratios, and proportions used herein are by weight of the composition unless otherwise specified. All average values ​​are calculated "by weight" of the composition unless otherwise expressly indicated. All ratios are calculated as weight / weight levels unless otherwise specified.

[0018] All measurements are performed at 25° C. unless otherwise specified.

[0019] Unless otherwise noted, all component or composition concentrations are in terms of the active portion of that component or composition and are exclusive of impurities, e.g., residual solvents or by-products, that may be present in commercial sources of such component or composition.

[0020] How to treat the surface The present disclosure relates to a method of treating a surface, which may be a hard or soft surface, preferably the surface is a soft surface, and more preferably the surface is a fabric. The surface comprises: a) bacterial spores, preferably Bacillus spores; b) Saccharomyces supernatant; and is processed.

[0021] For example, the method of the present disclosure may include contacting a fabric with a product according to the present disclosure. The contacting may occur, in whole or in part, in the presence of water. The product or a portion thereof may be diluted and / or dissolved in water to form a treatment solution.

[0022] The method of the present disclosure may include contacting a surface, preferably a fabric, with an aqueous treatment solution. The aqueous treatment solution has a total bacterial spore count of about 1×10 2 Colony forming units (CFU) ~ approx. 1 x 10 10 CFU, preferably about 1 x 10 3 C CFU ~ approx. 1 x 10 9 CFU, more preferably about 1×10 4 C CFU ~ approx. 1 x 10 8 The aqueous treatment liquid may contain Saccharomyces supernatant in an amount of about 0.001% by weight to about 10% by weight, preferably about 0.01% by weight to about 5% by weight of the liquid.

[0023] The method of treating fabrics can be carried out in whole or in part in any suitable container, for example in an automatic washing machine. Such machines can be top-loading or front-loading machines. The entire process can be carried out in the washing machine. Alternatively, part of the process can be carried out in the washing machine and part of the process can be carried out in the dryer. The method of the present invention is also suitable for hand-washing applications.

[0024] The treatment step may be part of the wash or rinse cycle of an automatic washing machine. The aqueous treatment solution may be an aqueous rinse solution. The product according to the present disclosure may be added to the drawer or drum of the automatic washing machine during the wash or rinse cycle.

[0025] The treatment step of the disclosed method may include contacting the fabric with an aqueous wash liquor. The step of contacting the fabric with the aqueous wash liquor may occur prior to contacting the fabric with the aqueous rinse liquor. Such a step may occur during a single treatment cycle. The aqueous wash liquor may include a cleaning composition, such as a granular or liquid laundry detergent composition that is dissolved or diluted in water. The detergent composition may include an anionic surfactant. The aqueous wash liquor may include from about 50 ppm to about 5000 ppm, or from about 100 ppm to about 1000 ppm, of an anionic surfactant.

[0026] The method of the present invention can include a laundry process including a washing, rinsing, and drying cycle, and the bacterial spores and / or Saccharomyces supernatant can be provided to the fabric separately or together from the washing composition and / or the additive composition. The Saccharomyces supernatant can be provided to the washing cycle. The bacterial spores can be provided to the washing cycle, the rinsing cycle, or the drying cycle. When the Saccharomyces supernatant is added to the washing cycle, it can form an aqueous solution containing about 0.01 to about 10 ppm, preferably about 0.05 to about 1 ppm, of Saccharomyces supernatant. The Saccharomyces supernatant can be provided from the washing composition.

[0027] The bacterial spores, preferably Bacillus spores, may be added from an additive composition at a concentration of about 0.01% to about 5% by weight of the fabric. Preferably, the bacterial spores are provided in the form of beads or from a dryer sheet. The Saccharomyces supernatant and the bacterial spores may be added from the same product.

[0028] The treated fabric may be a synthetic fabric. Suitable synthetic fabrics include polyester, acrylic, nylon, rayon, acetate, spandex, lastex, and / or orlon fabrics. The process of the present invention provides very good malodor removal and / or prevention on synthetic fabrics.

[0029] The treated fabric may comprise synthetic fibers. Suitable synthetic fibers may include polyester, acrylic, nylon, rayon, acetate, spandex, lastex, and / or Orlon fibers. The fibers may be elastic and / or contain elastane. The fabric may comprise a blend of synthetic and natural fibers (e.g., a polycotton blend). The fabric may comprise fibers that are relatively hydrophobic (e.g., compared to cotton fibers).

[0030] product The present disclosure relates to products for treating fabric surfaces, preferably fabric treatment products. As used herein, the phrase "fabric treatment products" includes compositions and formulations designed for the treatment of fabrics, such as clothing, or other textiles.

[0031] Such products may include, but are not limited to, laundry cleaning compositions and detergents, fabric softening compositions, fabric enhancing compositions, fabric deodorizing compositions, laundry pre-cleaning agents, laundry pre-treatment agents, laundry additives, spray products, dry cleaning agents or compositions, laundry rinse additives, cleaning additives, post-rinse fabric treatment agents, ironing aids, unit dose formulations, delayed delivery formulations, detergents contained on or in porous substrates or nonwoven sheets, and other suitable forms that may be apparent to those skilled in the art in light of the teachings herein.Such products may be used as laundry pre-treatment agents, laundry post-treatment agents, or may be added during the wash and / or rinse cycle of the laundry process.Alternatively, the product or a part thereof, particularly the composition containing bacterial spores, may be added to the dryer.

[0032] The product may be in any suitable form. The product may be in the form of a liquid composition, a granular composition, a single compartment pouch, a multi-compartment pouch, a sheet, a lozenge or bead, a fibrous article, a tablet, a bar, a flake, or a mixture thereof. The product may be selected from a liquid, a solid, or a combination thereof.

[0033] The product may be a liquid composition. The composition may comprise about 30% to about 90%, or about 50% to about 80% water by weight of the composition. The pH of the composition may be optimized to promote bacterial spores and Saccharomyces supernatant.

[0034] The product may be a cleaning or additive composition and may be in the form of a unitized dose article, such as a tablet, pouch, sheet, or fibrous article. Such pouches typically include a water-soluble film, such as a polyvinyl alcohol water-soluble film, that at least partially encapsulates the composition. Suitable films are available from MonoSol, LLC (Indiana, USA). The composition may be encapsulated in a single compartment pouch or a multi-compartment pouch. A multi-compartment pouch may have at least two, at least three, or at least four compartments. A multi-compartment pouch may include compartments arranged side-by-side and / or stacked. The composition contained in the pouch or its compartments may be liquid, solid (such as a powder), or a combination thereof. The pouch composition may have a relatively low amount of water, such as less than about 20% by weight of the detergent composition, or less than about 15% by weight, or less than about 12% by weight, or less than about 10% by weight, or less than about 8% by weight.

[0035] The product may be in the form of a tablet or beads. The tablet may contain polyethylene glycol as a carrier. The polyethylene glycol may have a weight average molecular weight of about 2000 to about 20,000 daltons, preferably about 5000 to about 15,000 daltons, and even more preferably about 6000 to about 12,000 daltons. Preferably, the tablet contains bacterial spores.

[0036] The product may include a non-aqueous solvent that may act as a carrier and / or promote stability. Non-aqueous solvents may include organic solvents such as methanol, ethanol, propanol, isopropanol, 1,3-propanediol, 1,2-propanediol, ethylene glycol, glycerin, glycol ethers, hydrocarbons, or mixtures thereof. Other non-aqueous solvents may include lipophilic fluids such as siloxanes or other silicones, hydrocarbons, perfluorinated amines, perfluorinated and hydrofluoroether solvents, or mixtures thereof. Amine-containing solvents such as monoethanolamine, diethanolamine, and triethanolamine may be suitable.

[0037] Bacterial spores Although bacterial spores may be present on a surface, the method of the present invention involves the deliberate addition of bacterial spores to a surface in an amount that can provide a significant benefit to the consumer, particularly the benefit of malodour removal and prevention. Preferably, the method of the present invention involves the intentional addition of bacterial spores to a surface in an amount that can provide a significant benefit to the consumer, particularly the benefit of malodour removal and prevention. 2 CFU / g, preferably at least 1 × 10 3 CFU / g, preferably at least 1 × 10 4 CFU / g, preferably at least 1 × 10 5 CFU / g, preferably 1×10 12 Intentional addition of bacterial spores is required, less than CFU / g. By "intentional addition of bacterial spores" herein it is meant that the spores are added in addition to any microorganisms that may be present on the fabric.

[0038] The microbial spores used in the methods and products of the present invention can be added to the wash, rinse, or dry cycle. The spores are not inactivated by heat at temperatures found in washing machines or dryers. The spores are fabric persistent and provide malodor control during and after the washing process, especially during and after the use (e.g., wearing) of the fabric. Another example is found in towels. Towels can become malodorous after being used and left in the moist environment of a bathroom. The bacterial spores combined with Saccharomyces fermentation filtrate provide continuous malodor control.

[0039] The microbial spores of the method and product of the present invention can germinate on the fabric. The spores can be activated by heat, for example, heat generated during the use of the fabric or heat provided in a washing machine or dryer. The spores can germinate when the fabric is stored and / or used. Malodor precursors can be used by the microorganisms produced by the spores as nutrients to promote germination.

[0040] The fabrics can be treated with a wet laundering process, or can be treated wet after washing, for example in a dryer, or sprayed. The washing process reduces the amount of microorganisms and metabolites on the fabric, but additional bacteria from the washing machine and wash water can be transferred to the fabric. Alternatively, the fabrics can be treated dry to refresh them.

[0041] Microbial spores for use herein are i) capable of surviving temperatures found in a dryer, ii) fabric persistent, iii) capable of controlling odor, and iv) preferably capable of supporting the cleaning action of a laundry detergent. The spores have the ability to germinate and form cells during processing and continue to germinate and form cells on the fabric using malodor precursors as nutrients. The spores can be provided in liquid or solid form. Preferably, the spores are in solid form. The spores can be provided to the drying process from a reservoir, dryer ball, solid carrier, such as a pouch, pellet, bead, tablet, dryer sheet, etc. Preferably, the pellets are substantially spherical and / or cylindrical and have a diameter of about 1 mm to about 30 mm. Preferably, the spores are provided from a dryer sheet.

[0042] Some Gram-positive bacteria have a two-stage life cycle. During their life cycle, bacteria growing under certain conditions, such as in response to nutrient-deficient conditions, can execute an elaborate developmental program that leads to spore or endospore formation. Bacterial spores are protected by a coat consisting of about 60 different proteins assembled as a biochemically complex structure with interesting morphological and mechanical properties. The protein coat is considered as a static structure that provides rigidity and acts mainly as a sieve to filter out exogenous large toxic molecules such as lytic enzymes. Spores play an important role in the long-term survival of a species since they are highly resistant to extreme environmental conditions. Spores can also remain metabolically dormant for many years. Methods for obtaining bacterial spores from vegetative cells are well known in the art. In some instances, vegetative bacterial cells are grown in liquid media. From late logarithmic growth phase or early stationary phase, bacteria can start sporulation. Once the bacteria finish sporulation, their spores can be obtained from the medium, for example, by using centrifugation. Various methods can be used to kill or remove any remaining vegetative cells. Various methods can be used to purify spores from cell debris and / or other materials or substances. Bacterial spores can be differentiated from vegetative cells using various techniques, such as phase contrast microscopy, automated scanning microscopy, high-resolution atomic force microscopy, or heat resistance methods. Bacterial spores are generally metabolically inactive or dormant, environmentally resistant structures, making them easily selected for use in commercial microbial products. Despite their hardiness and extremely long life span, spores can rapidly respond to the presence of certain small molecules known as germination, which signals favorable conditions for interrupting dormancy by germination, the initial step in the process of completing the life cycle by reverting back to a vegetative bacterium. For example, commercial microbial products can be designed such that the spores are dispersed in an environment where they encounter germs present in the environment, germinate within the vegetative cell, and perform their intended function. A variety of different bacteria can form spores. Bacteria from any of these groups can be used in the compositions, methods, and kits disclosed herein.For example, the following genera: Acetonema, Alkalibacillus, Ammoniphilus, Amphibacillus, Anaerobacter, Anaerospora, Aneuribacillus, Anoxybacillus, Bacillus, Brevibacillus, Cardanaerobacter, Caloramater, Caminicella, Serrasibacillus, Clostridium, Clostridium diisalibacter, Cornella, Dendrosporobacter, Desulfotomaculum, Desulfosporomusa, Desulfosporosinus. S., Desulfovirgra, Desulfnispora, Desulfrispora, Filifactor, Filobacillus, Gerulia, Geobacillus, Geosporobacter, Gracilibacillus, Halonatronum, Heliobacterium, Heliophyllum, Raceella, Lentibacillus, Raisinibacillus, Mahera, Metabacterium, Molella, Natroniella, Oceanobacillus, Olenia, Ornithinebacillus, Oxalophagus, Oxobacillus Paenibacillus, Paraliobacillus, Perospora, Perotomaculum, Piscibacillus, Planiphyllum, Pontibacillus, Propionispora, Salinibacillus, Sarsuginibacillus, Seinonella, Simazuela, Sporacetigenium, Sporoanaerobacter, Sporobacter, Sporobacterium, Sporohalobacter, Sporolactobacillus, Sporomusa, Sporosartia, Sporotalea, Sporotomaculum, Schizosaccharomyces cerevisiae ... Some bacteria, including Enterobacillus, Syntrophospora, Tenuibacillus, Tepidibacter, Teribacillus, Thalassobacillus, Thermoacetogenium, Thermoactinomyces, Thermoalkalibacillus, Thermoanaerobacter, Thermoanaeromonas, Thermobacillus, Thermoflavimicrobium, Thermovenablum, Tuberibacillus, Bulgibacillus, and / or Vulcanobacillus, may form spores.

[0043] Preferably, the bacterium capable of forming spores is a member of the Bacillaceae family, e.g., Aeribacillus, Allibacillus, Alkalibacillus, Alkalicoccus, Alkalihalobacillus, Alkalicactibacillus, Allobacillus, Alteribacillus, Alteribacter, Amphibacillus, Anaerobacillus, Anoxybacillus, Aquibacillus, Aquisaribacillus, Aureibacillus, Bacillus, Caldarcalibacillus, Caldibacillus, Calditericola, Caldifontisbacillus, Cameliibacillus, Serrasibacillus, Compostibacillus, Rus, Cytobacillus, Desertiibacillus, Domibacillus, Ectobacillus, Evansella, Farcibacillus, Ferdinandcohina, Fermentibacillus, Fictibacillus, Filobacillus, Geobacillus, Geomicrobium, Gottfriedia, Gracilibacillus, Hallalkalibacillus, Halobacillus, Halolactibacillus, Heindrixia, Hydrogenibacillus, Lederbergia, Lentibacillus, Richfieldia, Rottidebacillus, Margaritia, Marinococcus, Mergilibacillus, Mesobacillus, Metabacillus, Microaerobacter, Natribacillus, Natronobacillus, Neobacillus, Niaria, Oceanobacillus, Ornithinibacillus, Parageobacillus, Paraliobacillus, Paralcalibacillus, Paucisalibacillus, Pelagiabdos, Peribacillus, Piscibacillus, Polygonibacillus, Pontibacillus, Pradosia, Prieszia, Pseudogracilibacillus, Pueribacillus, Radiobacillus, Robertomuraya, Rosellomorea, Saccharococcus, Salibacterium, Salimicrobium, In various examples, the bacteria are from the species of the genus Bacillus, Bacillus aeruginii, Bacillus acicularis, Bacillus aeruginii, Bacillus serrulata ...Bacillus aerophilus, Bacillus albus, Bacillus alticudinis, Bacillus albeauensis, Bacillus amyloliquefaciensex, Bacillus anthracis, Bacillus aquiflavi, Bacillus atrophaeus, Bacillus australimalis, Bacillus badius, Bacillus benzoevorans, Bacillus cabriaresii, Bacillus canaverarius, Bacillus capparidis, Bacillus carboniphilus, Bacillus cereus, Bacillus chagangensis, Bacillus corefiliensis, Bacillus cytotoxicus, Bacillus decisifrondis, Bacillus ectoiniformans, Bacillus enculensis, Bacillus fengquensis, Bacillus fungorum, Bacillus glitinifermentans, Bacillus gobiensis, Bacillus halotolerans, Bacillus hynessii, Bacillus horti, Bacillus inaquosorum, Bacillus infantis, Bacillus infernus, Bacillus isaberiae, Bacillus quequeae, Bacillus licheniformis, Bacillus luti, Bacillus manusensis, Bacillus ma linisedimentorum, Bacillus mesophilus, Bacillus methanolicus, Bacillus mobilis, Bacillus mojavensis, Bacillus mycoides, Bacillus nakamurai, Bacillus nudiopicus, Bacillus nitratiredusens, Bacillus oleivorans, Bacillus pacificus, Bacillus pachystanensis, Bacillus paralicheniformis, Bacillus paramycoides, Bacillus paranthrasis, Bacillus pervagus, Bacillus pisticola, Bacillus proteolyticus, Bacillus pseudomycoides, Bacillus proteolyticus Mills, Bacillus safensis, Bacillus tharracetis, Bacillus salinus, Bacillus salitolatorens, Bacillus theohaeanensis, Bacillus sibazii, Bacillus siamensis, Bacillus smithii, Bacillus solimanglobi, Bacillus songkurensis, Bacillus sonorensis, Bacillus spizizenii, Bacillus spongiae, Bacillus stearcoris, Bacillus stratosphaerix, Bacillus subtilis, Bacillus swedseyi, Bacillus thaeanensis, Bacillus tamaricis, Bacillus tequilensis,The strain may be a strain of Bacillus thermocloacae, Bacillus thermotolerans, Bacillus thuringiensis, Bacillus tianshenii, Bacillus toyonensis, Bacillus tropicalis, Bacillus valismortis, Bacillus berezuensis, Bacillus viedmannii, Bacillus vdaliankiensis, Bacillus kiamenensis, Bacillus kiapuensis, Bacillus zangzouensis, or a combination thereof.

[0044] In some examples, the spore-forming bacterial strain may be a Bacillus strain, such as, for example, Bacillus spp. strain SD-6991, Bacillus spp. strain SD-6992, Bacillus spp. strain NRRL B-50606, Bacillus spp. strain NRRL B-50887, Bacillus pumilus strain NRRL B-50016, Bacillus amyloliquefaciens strain NRRL B-50017, Bacillus amyloliquefaciens strain PTA-7792 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50018, Bacillus amyloliquefaciens strain NRRL B-50019, Bacillus amyloliquefaciens strain NRRL B-50020, Bacillus amyloliquefaciens strain NRRL B-50021, Bacillus amyloliquefaciens strain NRRL B-50022, Bacillus amyloliquefaciens strain NRRL B-50023, Bacillus amyloliquefaciens strain NRRL B-50024, Bacillus amyloliquefaciens strain NRRL B-50025, Bacillus amyloliquefaciens strain NRRL B-50026, Bacillus amyloliquefaciens strain NRRL B-50027, Bacillus amyloliquefaciens strain NRRL B-50028, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-50026, Bacillus amy B-50018, Bacillus amyloliquefaciens strain PTA-7541, Bacillus amyloliquefaciens strain PTA-7544, Bacillus amyloliquefaciens strain PTA-7545, Bacillus amyloliquefaciens strain PTA-7546, Bacillus subtilis strain PTA-7547, Bacillus amyloliquefaciens strain PTA-7549, Bacillus amyloliquefaciens strain PTA-7793, Bacillus amyloliquefaciens strain PTA-7790, Bacillus amyloliquefaciens strain PTA-7791, Bacillus subtilis strain NRRL B-50136 (also known as DA-33R, ATCC Accession No. 55406), Bacillus amyloliquefaciens strain NRRL B-50141, Bacillus amyloliquefaciens strain NRRL B-50399, Bacillus licheniformis strain NRRL B-50014, Bacillus licheniformis strain NRRL B-50015, Bacillus amyloliquefaciens strain NRRL B-50607, Bacillus subtilis strain NRRL B-50147 (also known as 300R), Bacillus amyloliquefaciens strain NRRL B-50150, Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus megaterium PTA-3142, Bacillus amyloliquefaciens strain ATCC accession number 55405 (also known as 300), Bacillus amyloliquefaciens strain ATCC accession number 55407 (also known as PMX), Bacillus pumilus NRRL B-50398 (ATCCBacillus cereus ATCC Accession No. 700385, PMX-1, and NRRL B-50255), Bacillus cereus ATCC Accession No. 700386, Bacillus thuringiensis ATCC Accession No. 700387 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL B-50304 and NRRL B-50349 available from Novozymes TAEGRO®), Bacillus subtilis (e.g., isolate NRRL B-21661 in RHAPSODY®, SERENADE® MAX, and SERENADE® ASO available from Bayer CropScience), Bacillus pumilus (e.g., isolate NRRL B-21661 available from Bayer CropScience), Bacillus cereus ATCC Accession No. 700387, Bacillus thuringiensis ATCC Accession No. 700388 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL B-50304 and NRRL B-50349 available from Novozymes TAEGRO®), Bacillus subtilis (e.g., isolate NRRL B-21661 in RHAPSODY®, SERENADE® MAX, and SERENADE® ASO available from Bayer CropScience), Bacillus pumilus (e.g., isolate NRRL B-21661 available from Bayer CropScience), Bacillus cereus ATCC Accession No. 700389, Bacillus cereus ATCC Accession No. 7003 B-50349 isolate), Bacillus amyloliquefaciens TrigoCor (also known as "TrigoCor 1448", e.g., isolate having Embrapa Trigo accession number 144 / 88.4Lev, Cornell accession number Pma007BR-97, and ATCC accession number 202152 available from Cornell University, USA), and combinations thereof.

[0045] In some examples, the spore-forming bacterial strain can be a Bacillus amyloliquefaciens strain. For example, the strain can be Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), and / or Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50154, or from other Bacillus amyloliquefaciens microorganisms.

[0046] In some examples, the spore-forming bacterial strain may be a Brevibacillus species, such as Brevibacillus brevis, Brevibacillus formosus, Brevibacillus laterosporus, or Brevibacillus parabrevis, or a combination thereof.

[0047] In some examples, the spore-forming bacterial strain may be a Paenibacillus species, such as Paenibacillus alvei, Paenibacillus amylolyticus, Paenibacillus azotofixans, Paenibacillus cookii, Paenibacillus macerans, Paenibacillus polymyxa, or Paenibacillus validus, or a combination thereof. The bacterial spores may have an average particle size of about 2 to 50 microns, preferably about 10 to 45 microns. Bacillus spores are commercially available in blends in aqueous carriers in which they are insoluble. Other commercially available bacillus spore blends include, but are not limited to, Fenshen Free™ CAN (10X), available from Novozymes Biologicals, Inc., Evogen® Renew Plus (10X), available from Genesis Biosciences, Inc., and Evogen® GT (10X, 20X, and 110X), all available from Genesis Biosciences, Inc. In the preceding list, the designations in parentheses (10X, 20X, and 110X) indicate the relative concentrations of bacillus spores.

[0048] The bacterial spores used in the compositions, methods, and products disclosed herein may or may not be heat activated. In some examples, the bacterial spores are heat activated. In some examples, the bacterial spores are not heat inactivated. Preferably, the spores used herein are heat activated. Heat activation may include heating the bacterial spores from room temperature (15-25°C) to an optimum temperature of 25-120°C, preferably 40C-100°C, and holding the optimum temperature for up to 2 hours, preferably 30 minutes at 70-80°C.

[0049] For the methods, compositions, and products disclosed herein, a population of bacterial spores is generally used. In some examples, the population of bacterial spores may include bacterial spores from a single strain of bacteria. Preferably, the population of bacterial spores may include bacterial spores from two, three, four, five, or more strains of bacteria. In general, the population of bacterial spores contains a majority of spores and a small number of vegetative cells. In some examples, the population of bacterial spores does not contain vegetative cells. In some examples, the population of bacterial spores may contain less than about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% vegetative cells, and the percentage of bacterial spores is calculated as ((number of vegetative cells / (number of spores in the population+number of vegetative cells in the population))×100). Generally, the populations of bacterial spores used in the disclosed methods, compositions, and products are stable (i.e., not germinating) and at least some of the individual spores in the population are capable of germination.

[0050] The population of bacterial spores used in the present disclosure may contain different concentrations of bacterial spores. In various examples, the population of bacterial spores is at least 1×10 2 , 5×10 2 , 1×10 3 , 5×10 3 , 1×10 4 , 5×10 4 , 1×10 5 , 5×10 5 , 1×10 6 , 5×10 6 , 1×10 7 , 5×10 7, 1×10 8 , 5×10 8 , 1×10 9 , 5×10 9 , 1×10 10 , 5×10 10 , 1×10 11 , 5×10 11 , 1×10 12 , 5×10 12 , 1×10 13 , 5×10 13 , 1×10 14 , or 5 × 10 14 particles / mL, particles / gram, or particles / cm 3 The spores may include, but are not limited to, spores of

[0051] Dryer sheets can be conveniently used to treat fabrics during the drying process in a dryer. Dryer sheets can be used to treat unwashed fabrics or fabrics after they have been washed with laundry detergent.

[0052] Preferably, the bacterial spore comprises a Bacillus spore, more preferably a Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, Bacillus mojavensis), and mixtures thereof, and more preferably, the bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and mixtures thereof.

[0053] Saccharomyces Supernatant Saccharomyces supernatant is a material containing bacterial enzymes obtained by fermentation and filtration of Saccharomyces yeast. The supernatant can be obtained by any separation process including filtration, dialysis and centrifugation. Bacterial enzymes can utilize inorganic nitrogen, for example from ammonia, to form amino acids. Saccharomyces supernatant is an environmentally friendly compound since it can be derived from organic sources, for example from plant sources. Suitable Saccharomyces supernatants are supplied by Carrubba Incorporated, Milford, CT, USA. These include DeoPlex® DH (Saccharomyces fermentation filtrate), DeoPlex® Organic and DeoPlex® Clear.

[0054] The methods and products of the disclosure include Saccharomyces supernatant for the purpose of removing, inhibiting, and / or reducing malodors on a surface, preferably on fabric, for example, for inhibiting and / or reducing the development of malodors on washed damp fabrics over a period of time such as from about 1 hour to about 72 hours or more, or for example, for removing and / or reducing malodors resulting from exposing fabrics to a malodor source prior to washing.

[0055] The Saccharomyces supernatant may be provided as part of a cleaning composition or as part of an additive composition. Preferably, the composition is a laundry cleaning composition. The composition comprises the Saccharomyces supernatant in an amount of about 0.05 to about 10% by weight of the composition, such as about 0.05 to about 5% by weight, such as about 0.05 to about 2% by weight, such as about 0.05 to about 1% by weight, or about 0.05 to about 0.7% by weight.

[0056] Cleaning Composition Components Suitable cleaning ingredients include at least one of a surfactant, an enzyme, an enzyme stabilizing system, a detergent builder, a chelating agent, a complexing agent, a clay soil removal / anti-redeposition agent, a polymeric soil release agent, a polymeric dispersing agent, a polymeric grease cleaner, a dye transfer inhibitor, a bleaching agent, a bleach activator, a bleach catalyst, a fabric conditioner, a clay, a suds booster, a defoamer, a suds suppressor, a corrosion inhibitor, a soil suspending agent, a dye, a hueing dye, a disinfectant, an anti-tarnish agent, an optical brightener, a fragrance, a saturated or unsaturated fatty acid, a calcium cation, a magnesium cation, a visual signal component, a structurant, a thickener, an anti-caking agent, a starch, a sand, a gelling agent, or any combination thereof.

[0057] Surfactant System: The composition may include a surfactant system in an amount sufficient to provide the desired cleaning characteristics. In some embodiments, the composition includes from about 1% to about 70% of a surfactant system, by weight of the composition. In other embodiments, the liquid composition includes from about 2% to about 60% of a surfactant system, by weight of the composition. In further embodiments, the composition includes from about 5% to about 30% of a surfactant system, by weight of the composition. The surfactant system may include a detersive surfactant selected from anionic surfactants, nonionic surfactants, cationic surfactants, zwitterionic surfactants, amphoteric surfactants, ampholytic surfactants, and mixtures thereof. One skilled in the art will appreciate that a detersive surfactant includes any surfactant or mixture of surfactants that provides a cleaning, stain removal, or laundering benefit to soiled materials.

[0058] Anionic Surfactants. Non-limiting examples of suitable anionic surfactants include any conventional anionic surfactant, including linear alkylbenzene sulfonates (LAS), alpha-olefin sulfonates (AOS), alkyl sulfates (fatty alcohol sulfates) (AS), alcohol ethoxy sulfates (AEOS or AES), secondary alkane sulfonates (SAS), alpha-sulfo fatty acid methyl esters, alkyl- or alkenyl succinic acids, or soaps.

[0059] Suitable alkylbenzene sulfonates (LAS) can be obtained by sulfonating commercially available linear alkylbenzenes (LABs). Suitable LABs include low 2-phenyl LABs such as those supplied by Sasol under the trade name Isochem® or those supplied by Petresa under the trade name Petrelab®, and other suitable LABs include high 2-phenyl LABs such as those supplied by Sasol under the trade name Hyblene®. Suitable anionic detersive surfactants are alkylbenzene sulfonates obtained by the DETAL catalyzed process, although other synthetic routes such as HF may be suitable. In one embodiment, magnesium salts of LAS are used.

[0060] The detersive surfactant may be a mid-chain branched detersive surfactant, in one aspect a mid-chain branched anionic detersive surfactant, in one aspect a mid-chain branched alkyl sulfate and / or a mid-chain branched alkyl benzene sulfonate, such as a mid-chain branched alkyl sulfate. In one aspect, the mid-chain branching is 1~4 The alkyl groups are usually methyl and / or ethyl groups.

[0061] Other anionic surfactants useful herein are the water-soluble salts of paraffin sulfonates and secondary alkane sulfonates containing from about 8 to about 24 (and in some examples from about 12 to 18) carbon atoms; alkyl glyceryl ether sulfonates, especially C 8~18 Ethers of alcohols (e.g., derived from tallow and coconut oil). Mixtures of alkylbenzene sulfonates with the above-mentioned paraffin sulfonates, secondary alkane sulfonates, and alkyl glyceryl ether sulfonates are also useful. Further suitable anionic surfactants include methyl ester sulfonates and alkyl ether carboxylates (AECs).

[0062] Suitable anionic surfactants also include branched anionic surfactants selected from branched sulfate or branched sulfonate surfactants. Further suitable branched anionic detersive surfactants include surfactants derived from alcohols branched at the 2-alkyl position, which are sold under the trade names Isalchem® 123, Isalchem® 125, Isalchem® 145, Isalchem® 167, etc., and are derived from the oxo process. Due to the oxo process, the branch is located at the 2-alkyl position. These 2-alkyl branched alcohols usually range in length from C11 to C14 / C15, and all include structural isomers branched at the 2-alkyl position.

[0063] Anionic surfactants may be present in acid form, and the acid form may be neutralized to form surfactant salts. Typical neutralizing agents include metal counterion bases such as hydroxides, e.g., NaOH or KOH. Further suitable neutralizing agents for neutralizing these acid form anionic surfactants include ammonia, amines, or alkanolamines. Non-limiting examples of alkanolamines include monoethanolamine, diethanolamine, triethanolamine, and other linear or branched alkanolamines known in the art. Suitable alkanolamines include 2-amino-1-propanol, 1-aminopropanol, monoisopropanolamine, or 1-amino-3-propanol. Amine neutralization may be complete or partial, for example, a portion of the anionic surfactant mixture may be neutralized with sodium or potassium, and a portion of the anionic surfactant mixture may be neutralized with amines or alkanolamines.

[0064] Nonionic surfactants. Suitable nonionic surfactants useful herein can include any conventional nonionic surfactant. These can include, for example, alkoxylated fatty alcohols, and amine oxide surfactants. Other non-limiting examples of nonionic surfactants useful herein include C8-C 18 Alkyl ethoxylates (NEODOL® nonionic surfactants (Shell), etc.); C6-C 12 Alkylphenol alkoxylates (the alkoxylate units can be ethyleneoxy units, propyleneoxy units, or combinations thereof); C 12 ~C 18 C6-C with alcohol and ethylene oxide / propylene oxide block polymer 12 Alkylphenol condensates (such as Pluronic® (BASF)); C 14 ~C 22 Medium-chain branched alcohol (BA); C 14 ~C 22 Medium chain branched MEA (BAEx ), where x is 1 to 30); alkyl polysaccharides; specifically alkyl polyglycosides; polyhydroxy fatty acid amides; and ether-terminated poly(oxyalkylated) alcohol surfactants. Suitable nonionic detersive surfactants also include alkyl polyglucosides and alkyl alkoxylated alcohols. Suitable nonionic surfactants also include those sold by BASF under the trade name Lutensol®.

[0065] Cationic surfactants. The surfactant system may include a cationic surfactant. In some embodiments, the surfactant system includes from about 0% to about 7%, from about 0.1% to about 5%, or from about 1% to about 4% by weight of the surfactant system of a cationic surfactant, for example as a co-surfactant. In some embodiments, the compositions of the present invention are substantially free of cationic surfactants and surfactants that become cationic at a pH below 7 or below 6. Non-limiting examples of cationic surfactants include quaternary ammonium surfactants, which may have 26 or fewer carbon atoms, including alkoxylate quaternary ammonium (AQA) surfactants; dimethylhydroxyethyl quaternary ammonium; dimethylhydroxyethyl lauryl ammonium chloride; polyamine cationic surfactants; cationic ester surfactants; and amino surfactants, such as amidopropyl dimethylamine (APA). Suitable cationic detersive surfactants also include alkyl pyridinium compounds, alkyl quaternary ammonium compounds, alkyl quaternary phosphonium compounds, alkyl tertiary sulfonium compounds, and mixtures thereof.

[0066] Zwitterionic surfactants. Examples of zwitterionic surfactants include secondary and tertiary amine derivatives, heterocyclic secondary and tertiary amine derivatives, or derivatives of quaternary ammonium compounds, quaternary phosphonium compounds, or tertiary sulfonium compounds. Betaines, including alkyl dimethyl betaines and cocodimethylamidopropyl betaines, C8-C 18 (For example, C 12 ~C 18) amine oxides, and sulfo and hydroxy betaines such as N-alkyl-N,N-dimethylamino-1-propanesulfonates (wherein the alkyl group is C8 to C 18 In particular embodiments, C 10 ~C 14 (It can be said that.)

[0067] Amphoteric surfactants. Examples of amphoteric surfactants include aliphatic derivatives of secondary or tertiary amines, heterocyclic secondary and tertiary amines, where the aliphatic group may be linear or branched, and where one of the aliphatic substituents contains at least about 8 carbon atoms, typically about 8 to about 18 carbon atoms, and where at least one of the aliphatic substituents contains an anionic water-solubilizing group, such as carboxy, sulfonate, sulfate. Examples of compounds falling within this definition are sodium 3-(dodecylamino)propionate, sodium 3-(dodecylamino)propane-1-sulfonate, sodium 2-(dodecylamino)ethyl sulfate, sodium 2-(dimethylamino)octadecanoate, disodium 3-(N-carboxymethyldodecylamino)propane-1-sulfonate, disodium octadecyl-iminodiacetate, sodium 1-carboxymethyl-2-undecylimidazole, and sodium N,N-bis(2-hydroxyethyl)-2-sulfato-3-dodecoxypropylamine.Suitable amphoteric surfactants also include sarcosinates, glycinates, taurinates, and mixtures thereof.

[0068] Enzymes. Preferably, the composition comprises one or more enzymes. Preferred enzymes provide cleaning performance and / or fabric care benefits. Examples of suitable enzymes include, but are not limited to, hemicellulase, peroxidase, protease, cellulase, xylanase, lipase, phospholipase, esterase, cutinase, pectinase, mannanase, galactanase, pectate lyase, keratinase, reductase, oxidase, phenoloxidase, lipoxygenase, ligninase, pullulanase, tannase, pentosanase, malanase, β-glucanase, arabinosidase, hyaluronidase, chondroitinase, laccase, and amylase, or mixtures thereof. A typical combination is an enzyme cocktail that may include, for example, protease and lipase together with amylase.

[0069] Proteases. Preferably, the composition comprises one or more proteases. Suitable proteases include metalloproteases and serine proteases, including neutral or alkaline microbial serine proteases, such as subtilisin (EC 3.4.21.62). Suitable proteases include those of animal, vegetable or microbial origin. In one aspect, such suitable proteases may be of microbial origin. Suitable proteases include chemically or genetically modified variants of the aforementioned suitable proteases. In one aspect, suitable proteases may be serine proteases, such as alkaline microbial proteases or / and trypsin-type proteases. Examples of suitable neutral or alkaline proteases include: (a) Subtilisin (EC3.4.21.62), in particular WO 2004067737, WO 2015091989, WO 2015091990, WO 2015024739, WO 2015143360, U.S. Pat. No. 6,312,936 (B1), U.S. Pat. No. 5,679,630, U.S. Pat. No. 4,760,025, German Patent Publication No. 102006022216 (A1), German Patent Publication No. 102006022224 (A1), WO 2015089447, WO 2015089441, WO 2016066756 ... those derived from Bacillus species such as B. lentus, B. alkalophilus, B. subtilis, B. amyloliquefaciens, B. pumilus, B. gibsonii, and B. akibaii, as described in US Pat. Nos. 016066757, 2016069557, 2016069563, and 2016069569; (b) trypsin- or chymotrypsin-type proteases, such as trypsin (e.g., of porcine or bovine origin), including the Fusarium protease described in WO 89 / 06270, and the chymotrypsin protease derived from Cellumonas, described in WO 05 / 052161 and WO 05 / 052146. (c) metalloproteases, in particular those derived from Bacillus amyloliquefaciens, as described in WO 07 / 044993(A2). those derived from Bacillus, Brevibacillus, Thermoactinomyces, Geobacillus, Paenibacillus, Lysinibacillus or Streptomyces spp. as described in WO 2014194032, WO 2014194054 and WO 2014194117, Kribella alluminosa as described in WO 2015193488, and Streptomyces and Lysobacter as described in WO 2016075078. (d) proteases having at least 90% identity to the subtilase from Bacillus sp. TY145, NCIMB 40339, described in WO 92 / 17577 (Novozymes A / S), including variants of this Bacillus sp TY145 subtilase described in WO 2015024739 and WO 2016066757.

[0070] Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, Savinase®, Primase®, Durazym®, Polarzyme®, Kannase®, Liquanase®, Liquase Ultra®, Savinase Ultra®, Ovozyme®, Neutrase®, Everlase® and Esperase® by Novozymes A / S (Denmark); those sold under the trade names Maxatase®, Maxacal®, Maxapem®, Properase®, Purafect®, Purafect Prime®, Purafect Ox®, FN3®, FN4®, Excellase® and Purafect OXP® by Dupont; those sold by Enzymes, available from Henkel / Kemira, namely, BLAP (sequence shown in FIG. 29 of U.S. Pat. No. 5,352,604), and KAP (Bacillus alkalophilus subtilisin having the mutations A230V+S256G+S259N) available from Kao.

[0071] Amylase. Preferably, the composition may comprise an amylase. Suitable α-amylases include those of bacterial or fungal origin. Chemically or genetically modified variants are included. Preferred alkaline α-amylases are those derived from Bacillus species, such as Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus stearothermophilus, Bacillus subtilis, or other Bacillus sp., such as Bacillus NCIB 12289, NCIB 12512, NCIB 12513, DSM 9375 (U.S. Pat. No. 7,153,818), DSM 12368, DSMZ12649, KSM AP1378 (WO 97 / 00324), KSM K36, or KSM K38 (EP 1,022,334). (a) The variants described in WO 94 / 02597, WO 94 / 18314, WO 96 / 23874 and WO 97 / 43424, in particular variants in which one or more of the following positions have been substituted relative to the enzyme listed as SEQ ID NO:2 in WO 96 / 23874: 15, 23, 105, 106, 124, 128, 133, 154, 156, 181, 188, 190, 197, 202, 208, 209, 243, 264, 304, 305, 391, 408 and 444. (b) the variants described in U.S. Pat. No. 5,856,164, and in WO 99 / 23211, WO 96 / 23873, WO 00 / 60060 and WO 06 / 002643, in particular the AA560 enzyme listed as SEQ ID NO: 12 in WO 06 / 002643, at the following positions: 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 461, 471, 482, 484, preferably D183 * and G184 * Mutants that also contain a deletion of (c) SEQ ID NO: 4 in WO 06 / 002643, variants exhibiting at least 90% identity to the wild-type enzyme from Bacillus sp. SP722, in particular variants having deletions at positions 183 and 184, and variants described in WO 00 / 60060, which are incorporated herein by reference. (d) variants exhibiting at least 95% identity with the wild-type enzyme of Bacillus sp. 707 (SEQ ID NO: 7 in U.S. Pat. No. 6,093,562), in particular those comprising one or more of the following mutations M202, M208, S255, R172, and / or M261. Preferably, the amylase comprises one or more of M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N and / or R172Q. Particularly preferred are those comprising the M202L or M202T mutations. (e) a variant described in WO 09 / 149130, preferably one exhibiting at least 90% identity to SEQ ID NO:1 or SEQ ID NO:2 in WO 09 / 149130, a wild-type enzyme derived from Geobacillus Stearophermophilus or a truncated version thereof. (f) A variant exhibiting at least 89% identity to SEQ ID NO: 1 in WO2016091688, in particular one containing a deletion at positions H183+G184 and further containing one or more mutations at positions 405, 421, 422, and / or 428. (g) a variant exhibiting at least 60% amino acid sequence identity with “PcuAmyl α-amylase” from Paenibacillus curdlanolyticus YK9 (SEQ ID NO: 3 in WO 2014099523); (h) a variant exhibiting at least 60% amino acid sequence identity with "CspAmy2 amylase" from Cytophaga sp. (SEQ ID NO: 1 of WO 2014164777); (i) a variant exhibiting at least 85% identity to AmyE from Bacillus subtilis (SEQ ID NO: 1 of WO 2009149271); (j) A mutant having at least 90% identity with the wild-type amylase derived from Bacillus sp. KSM-K38 under accession number AB051102.

[0072] Suitable commercially available α-amylases include DURAMYL®, LIQUEZYME®, TERMAMYL®, TERMAMYL ULTRA®, NATALASE®, SUPRAMYL®, STAINZYME®, STAINZYME PLUS®, FUNGAMYL®, and BAN® (Novozymes A / S, Bagsvaerd, Denmark), KEMZYM® AT 9000 (Biozym Biotech Trading GmbH, Wehlistrasse 27b A-1200 Wien Austria), RAPIDASE®, PURASTAR®, ENZYSIZE®, OPTISIZE HT PLUS®, POWERASE®, and PURASTAR OXAM® (Genencor International Inc., Palo Alto, CA). Alto, California), and KAM® (Kao, 14-10 Nihonbashi Kayabacho, 1-chome, Chuo-ku Tokyo 103-8210, Japan). In one aspect, suitable amylases include NATALASE®, STAINZYME®, and STAINZYME PLUS®, and mixtures thereof.

[0073] Lipase. Preferably, the composition comprises one or more lipases, including "first cycle lipases" such as those described in U.S. Pat. No. 6,939,702 (B1) and U.S. Patent Application Publication No. 2009 / 0217464. A preferred lipase is a first wash lipase. The composition may comprise a first wash lipase.

[0074] The first wash lipase includes a lipase that is a polypeptide having the following amino acid sequence: (a) Humicola lanuginosa lipase (b) comprises, as compared to the wild-type lipase, a substitution of an electrically neutral or negatively charged amino acid on the surface of the three-dimensional structure within 15 A of E1 or Q249 with a positively charged amino acid, and (c) comprises a peptide addition at the C-terminus, and / or (d) comprises a peptide addition at the N-terminus, and / or (e) satisfies the following restrictions: i) comprises a negative amino acid at position E210 of the wild-type lipase, (ii) comprises a negatively charged amino acid in the region corresponding to positions 90-101 of the wild-type lipase, and (iii) comprises a neutral or negative amino acid at N94 or the position corresponding to the wild-type lipase, and / or has a negative or neutral net charge in the region corresponding to positions 90-101 of the wild-type lipase.

[0075] Preferred are variants of wild-type lipase from Thermomyces lanuginosus, including one or more of the mutations T231R and N233R. The wild-type sequence is Swiss-Prot accession number Swiss-Prot O59952 (269 amino acids (amino acids 23 to 291) from Thermomyces lanuginosus (Humicola lanuginosus)). Preferred lipases include those sold under the trade names Lipex®, Lipolex® and Lipoclean®.

[0076] Cellulases. Suitable cellulases are of bacterial or fungal origin. They include chemically modified or genetically engineered variants of proteins. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, and Acremonium, such as fungal cellulases produced from Humicola insolens, Myceliophthora thermophila, and Fusarium oxysporum, as disclosed in U.S. Pat. Nos. 4,435,307, 5,648,263, 5,691,178, 5,776,757, and 5,691,178. Suitable cellulases include alkaline or neutral cellulases with color care properties. Commercially available cellulases include CELLUZYME®, CAREZYME®, and CAREZYME PREMIUM (Novozymes A / S), CLAZINASE®, and PURADAX HA® (Genencor International Inc.), KAC-500(B)® (Kao Corporation).

[0077] The bacterial cleaning cellulase may be a glycosyl hydrolase having enzymatic activity against an amorphous cellulose substrate, the glycosyl hydrolase being selected from GH family 5, 7, 12, 16, 44 or 74. Suitable glycosyl hydrolases may also be selected from GH family 44 glycosyl hydrolases (wild type) from Paenibacillus polyxyma, such as XYG1006 described in U.S. Pat. No. 7,361,736, or variants thereof. GH family 12 glycosyl hydrolases (wild type) from Bacillus licheniformis, such as SEQ ID NO: 1 as described in U.S. Pat. No. 6,268,197, or variants thereof; GH family 5 glycosyl hydrolases (wild type) from Bacillus agaradhaerens, or variants thereof; GH family 5 glycosyl hydrolases (wild type) from Paenibacillus, such as XYG1034 and XYG1022 as described in U.S. Pat. No. 6,630,340, or variants thereof; GH family 74 glycosyl hydrolases (wild type) from Jonesia sp, such as XYG1020 as described in WO 2002 / 077242, or variants thereof; and Trichoderma reesei, such as the enzyme described in more detail in U.S. Pat. No. 7,172,891, SEQ ID NO: 2. Reesei), GH family 74 glycosyl hydrolase (wild type), or a variant thereof. Suitable bacterial cleaning cellulases are sold under the trade names Celluclean® and Whitezyme® (Novozymes A / S, Bagsvaerd, Denmark).

[0078] The composition may include a fungal cleaning cellulase belonging to family 45 of glycosyl hydrolases having a molecular weight between 17 kDa and 30 kDa, such as endoglucanases sold under the trade names Biotouch® NCD, DCC, and DCL (AB Enzymes, Darmstadt, Germany).

[0079] Pectate lyase. Other preferred enzymes include pectate lyases sold under the trade names Pectawash®, Pectaway®, Xpect®, and mannases sold under the trade names Mannaway® (all from Novozymes A / S, Bagsvaerd, Denmark), and Purabrite® (from Genencor International Inc., Palo Alto, California).

[0080] Nuclease enzyme. The composition may comprise a nuclease enzyme. A nuclease enzyme is an enzyme capable of cleaving phosphodiester bonds between nucleotide subunits of nucleic acids. The nuclease enzyme herein is preferably a deoxyribonuclease or ribonuclease enzyme or a functional fragment thereof. By functional fragment or portion is meant the portion of the nuclease enzyme that catalyzes the cleavage of phosphodiester bonds in the DNA backbone, and thus is the region of the nuclease protein that retains catalytic activity. It therefore includes truncated but functional versions of the enzyme and / or variants and / or derivatives and / or homologs in which functionality is maintained.

[0081] Preferably, the nuclease enzyme is a deoxyribonuclease preferably selected from any of the following classes: EC 3.1.21.x (wherein x=1, 2, 3, 4, 5, 6, 7, 8 or 9), EC 3.1.22.y (wherein y=1, 2, 4 or 5), EC 3.1.30.z (wherein z=1 or 2), EC 3.1.31.1, and mixtures thereof.

[0082] Mannanase. The composition may include an extracellular polymer degrading enzyme, including a mannanase enzyme. The term "mannanase" refers to a polypeptide having mannan endo-1,4-β-mannosidase activity (EC 3.2.1.78) from glycoside hydrolase family 26, which catalyzes the hydrolysis of 1,4-3-D-mannosidic bonds in mannans, galactomannans, and glucomannans. Alternative names for mannan endo-1,4-β-mannosidase are 1,4-3-D-mannan mannanohydrolase, endo-1,4-3-mannanase, endo-β-1,4-mannase, β-mannanase B, 3-1,4-mannan 4-mannanohydrolase, endo-3-mannanase, and β-D-mannanase. For purposes of this disclosure, mannanase activity may be determined using the reduced endo assay described in the experimental section of WO2015040159. Suitable examples from classification EC 3.2.1.78 are described in WO2015040159, such as the mature polypeptide SEQ ID NO: 1 described herein.

[0083] Galactanases. The composition may include extracellular polymer degrading enzymes, including endo-beta-1,6-galactanase enzymes. The term "endo-beta-1,6-galactanase" or "polypeptide having endo-beta-1,6-galactanase activity" refers to endo-beta-1,6-galactanase activity (EC 3.2.1.164) from glycoside hydrolase family 30 that catalyzes the hydrolytic cleavage of 1,6-3-D-galactooligosaccharides with a degree of polymerization (DP) higher than 3 and their acidic derivatives bearing a 4-O-methyl glucosyl uronate or glucosyl uronate group at the non-reducing end. For the purposes of this disclosure, endo-beta-1,6-galactanase activity is determined in Assay I according to the procedure described in WO2015185689. Suitable examples from the classification EC3.2.1.164 are described in WO2015185689, e.g., mature polypeptide SEQ ID NO:2.

[0084] Enzyme stabilization system. The composition may optionally comprise from about 0.001% to about 10% by weight of the composition of an enzyme stabilization system. The enzyme stabilization system may be any stabilization system compatible with the detersive enzymes. In the case of aqueous detergent compositions containing proteases, reversible protease inhibitors such as boron compounds, including borate, 4-formylphenylboronic acid, phenylboronic acid, and derivatives thereof, or compounds such as calcium formate, sodium formate, and 1,2-propanediol may be added to further improve stability.

[0085] Builder. The composition may optionally include a builder or builder system. Built cleaning compositions typically include at least about 1% builder by weight, based on the total weight of the composition. Liquid cleaning compositions may include up to about 10%, and in some instances up to 8%, builder by weight of the composition. Granular cleaning compositions may include up to about 30%, and in some instances up to 5%, builder by weight of the composition.

[0086] Builders selected from aluminosilicates (e.g. zeolite builders such as zeolite A, zeolite P, and zeolite MAP) and silicates assist in controlling the mineral hardness of the wash water, especially calcium and / or magnesium, or in removing particulate soils from surfaces. Suitable builders may be selected from the group consisting of phosphates, such as polyphosphates (e.g. sodium tri-polyphosphate), especially the sodium salts thereof; carbonates, bicarbonates, sesquicarbonates, and carbonate minerals other than sodium carbonate or sesquicarbonates; organic mono-, di-, tri-, and tetracarboxylates, especially water-soluble non-surfactant carboxylates in the form of acid, sodium, potassium, or alkanolammonium salts, and oligomeric or water-soluble low molecular weight polymeric carboxylates, including aliphatic and aromatic types, and phytic acid. These may be complemented, for example, by borates for pH buffering purposes, or by sulfates, especially sodium sulfate, and any other fillers or carriers that may be important in the engineering of a stable surfactant- and / or builder-containing cleaning composition. Additional suitable builders may be selected from citric acid, lactic acid, fatty acids, polycarboxylate builders, such as copolymers of acrylic acid, copolymers of acrylic acid and maleic acid, and copolymers of acrylic acid and / or maleic acid and other suitable ethylenic monomers with various types of additional functional groups. Also suitable for use as builders herein are synthetic crystalline ion exchange materials or hydrates thereof having a chain structure and a composition represented by the following general anhydrous form x(M2O)·ySiO2·zM'O, where M is Na and / or K, M' is Ca and / or Mg, y / x is 0.5-2.0, and z / x is 0.005-1.0.

[0087] Alternatively, the composition may be substantially free of builders.

[0088] Chelating Agents. The compositions may also include one or more metal ion chelating agents. Suitable molecules include copper, iron, and / or manganese chelating agents and mixtures thereof. Such chelating agents may be selected from the group consisting of phosphonates, aminocarboxylates, aminophosphonates, succinates, polyfunctionally substituted aromatic chelating agents, 2-pyridinol-N-oxide compounds, hydroxamic acids, carboxymethyl inulin, and mixtures thereof. The chelating agents may be present in the form of an acid or a salt, including alkali metal salts, ammonium salts, and substituted ammonium salts thereof, and mixtures thereof.

[0089] Aminocarboxylates useful as chelating agents include ethylenediaminetetracetate (EDTA), N-(hydroxyethyl)ethylenediaminetriacetate (HEDTA), nitrilotriacetate (NTA), ethylenediaminetetrapropionate, triethylenetetraaminehexaacetate, diethylenetriamine-pentaacetate (DTPA), methylglycinediacetic acid (MGDA), glutamic acid diacetate (GLDA), ethanol diglycine, triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (ETA), and the like. acid, EDTP), and derivatives thereof.

[0090] Carboxylate polymer. The composition may include one or more carboxylate polymers as a polymeric dispersant, anti-redeposition agent, or cleaning polymer. The carboxylate polymer may include at least one monomer selected from acrylic acid, maleic acid (or maleic anhydride), fumaric acid, itaconic acid, aconitic acid, mesaconic acid, citraconic acid, methylenemalonic acid, and any mixture thereof. In some embodiments, suitable carboxylate polymers include maleate / acrylate random copolymers or polyacrylate homopolymers.

[0091] In another embodiment, the carboxylate polymer may further comprise other monomers. Suitable other monomers may include sulfonated monomers such as 2-acrylamido-2-methylpropanesulfonic acid (AMPS), 2-(meth)acrylamido-2-methylpropanesulfonic acid, 4-styrenesulfonic acid, vinylsulfonic acid, 3-allyloxy, 2-hydroxy-1-propanesulfonic acid (HAPS), 2-sulfoethyl(meth)acrylic acid, 2-sulfopropyl(meth)acrylic acid, 3-sulfopropyl(meth)acrylic acid, and 4-sulfobutyl(meth)acrylic acid, and salts thereof.

[0092] Suitable other monomers can also include hydrophobically modified monomers such as alkyl acrylates, or monomers represented by formulas (I) and (II).

[0093] [ka] In formula (I), R0 represents a hydrogen atom or a CH3 group, R represents a CH2 group, a CH2CH2 group, or a single bond, X represents a number from 0 to 5, provided that when R is a single bond, X represents a number from 1 to 5, and R1 represents a hydrogen atom or a C1 to C20 organic group;

[0094] [ka] In formula (II), R0 represents a hydrogen atom or a CH3 group, R represents a CH2 group, a CH2CH2 group or a single bond, X represents a number from 0 to 5, and R1 represents a hydrogen atom or a C1 to C 20 It is an organic group.

[0095] An amphiphilic cleaning polymer. The composition has the following general structure: Bis((C2H5O)(C2H4O)n)(CH3)-N + -C x H 2x -N + The composition may include one or more amphiphilic cleaning polymers, such as a compound having the formula -(CH3)-bis((C2H5O)(C2H4O)n), where n=20-30 and x=3-8, or a sulfated or sulfonated variant thereof.

[0096] The composition may include amphiphilic alkoxylated grease cleaning polymers that have balanced hydrophilic and hydrophobic properties to remove grease particles from fabrics and surfaces. Specific embodiments of amphiphilic alkoxylated grease cleaning polymers include a core structure and a plurality of alkoxylate groups attached to the core structure. These may include alkoxylated polyalkyleneimines, for example, having an inner polyethylene oxide block and an outer polypropylene oxide block.

[0097] Alkoxylated polyamines can be used for grease and particulate removal. Such compounds can include, but are not limited to, ethoxylated polyethyleneimine, ethoxylated hexamethylenediamine, and sulfated versions thereof. Polypropoxylated derivatives can also be included. A wide variety of amines and polyalkyleneimines can be alkoxylated to various degrees. A useful example is the 600 g / mole polyethyleneimine core, which is ethoxylated to 20 EO groups per NH, available from BASF.

[0098] The cleaning composition may comprise a hydrophilic backbone comprising monomers such as, for example, unsaturated C1-C6 carboxylic acids, ethers, alcohols, aldehydes, ketones, esters, sugar units, alkoxy units, maleic anhydride, saturated polyalcohols such as glycerol, and mixtures thereof, and one or more C4-C 25 Mention may be made of random graft polymers containing hydrophobic side chains such as alkyl groups, polypropylene, polybutylene, vinyl esters of saturated C1-C6 monocarboxylic acids, C1-C6 alkyl esters of acrylic or methacrylic acid, and mixtures thereof. Specific examples of such graft polymers based on polyalkylene oxides and vinyl esters, especially vinyl acetate. These polymers are typically prepared by polymerizing vinyl esters in the presence of polyalkylene oxides, with the initiator being dibenzoyl peroxide, dilauroyl peroxide, or diacetyl peroxide.

[0099] The cleaning composition may contain blocks of ethylene oxide, propylene oxide. Examples of such block polymers include ethylene oxide-propylene oxide-ethylene oxide (EO / PO / EO) triblock copolymers, where the copolymer comprises a first EO block, a second EO block, and a PO block, and the first EO block and the second EO block are connected to the PO block. The blocks of ethylene oxide, propylene oxide, butylene oxide may also be arranged in other ways, such as (EO / PO) diblock copolymers, (PO / EO / PO) triblock copolymers, etc. The block polymer may also contain additional butylene oxide (BO) blocks.

[0100] Cellulosic Polymer The composition may comprise from about 0.1% to about 10% of a cellulosic polymer, by weight of the composition.

[0101] Suitable cellulosic polymers include alkyl cellulose, alkyl alkoxy alkyl cellulose, carboxy alkyl cellulose, and alkyl carboxy alkyl cellulose. In some embodiments, the cellulosic polymer is selected from carboxymethyl cellulose, methyl cellulose, methyl hydroxyethyl cellulose, methyl carboxymethyl cellulose, and mixtures thereof. In some embodiments, the cellulosic polymer is carboxymethyl cellulose having a degree of carboxymethyl substitution of about 0.5 to about 0.9 and a molecular weight of about 100,000 Da to about 300,000 Da.

[0102] Carboxymethylcellulose polymers include hydrophobically modified carboxymethylcelluloses such as Finnfix® GDA (sold by CP Kelko), an alkyl ketene dimer derivative of carboxymethylcellulose sold, for example, under the trade name Finnfix® SH1 (CP Kelko), or a block-based carboxymethylcellulose sold under the trade name Finnfix® V (sold by CP Kelko).

[0103] Suitable cellulose-based polymers also include cellulose polymers with cationic and / or hydrophilic modifications.Suitable cationic modified cellulose polymers include UCARE JR125, UCARE JR400, UCARE JR30M, UCARE LR400, UCARE LR30M, SOFTCAT SL-5, SOFTCAT SL-30, SOFTCAT SL-60, SOFTCAT SL-100, SOFTCAT SX-400X, SOFTCAT SX-1300H, SOFTCAT SX-1300X, SOFTCAT SK-H, and SOFTCAT SK-MH, all of which are sold by The Dow Chemical.

[0104] Additional amines: Various additional amines may be used in the composition to enhance the removal of grease and particles from soiled materials. The composition may include from about 0.1% to about 10%, in some examples from about 0.1% to about 4%, and in other examples from about 0.1% to about 2% additional amines by weight of the cleaning composition. Non-limiting examples of additional amines may include, but are not limited to, polyamines, oligoamines, triamines, diamines, pentamines, tetraamines, or combinations thereof. Specific examples of suitable additional amines include tetraethylenepentamine, triethylenetetraamine, diethylenetriamine, or mixtures thereof.

[0105] Dye transfer inhibitors. The composition may further comprise one or more dye transfer inhibitors. Suitable dye transfer inhibitors include, for example, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidone, polyvinylimidazole, manganese phthalocyanine, peroxidase, polyvinylpyrrolidone polymers, ethylenediaminetetraacetic acid (EDTA); diethylenetriaminepentamethylenephosphonic acid (DTPMP); hydroxyethanediphosphonic acid (HEDP); ethylenediamine N,N'-disuccinic acid (EDDS); methylglycinediacetic acid (MGDA); diethylenetriaminepentaacetic acid (DTPA); propylenediaminetetraacetic acid (PDT A); 2-hydroxypyridine-N-oxide (HPNO); or methylglycine diacetate (MGDA); glutamic acid N,N-diacetate (N,N-dicarboxymethylglutamic acid tetrasodium salt (GLDA); nitrilotriacetic acid (NTA); 4,5-dihydroxy-m-benzenedisulfonic acid; citric acid and any salts thereof; N-hydroxyethylethylenediaminetriacetic acid (HEDTA), triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (EDTP), and derivatives thereof, or combinations thereof.

[0106] Bleaching Compounds, Bleaching Agents, Bleach Activators, and Bleach Catalysts. The compositions described herein may include a bleaching agent, a bleach activator, and / or a bleach catalyst. The bleaching component may be present in a concentration of about 1% to about 30% by weight, and in some examples, about 5% to about 20% by weight, based on the total weight of the composition. If present, the amount of bleach activator may be about 0.1% to about 60% by weight, and in some examples, about 0.5% to about 40% by weight of the composition.

[0107] Examples of bleaching agents include oxygen bleaches, perborate bleaches, percarboxylic acid bleaches and their salts, peroxygen bleaches, persulfate bleaches, percarbonate bleaches, and mixtures thereof.

[0108] In some examples, the composition may also include a transition metal bleach catalyst.

[0109] Bleaching agents other than oxygen bleaches are also known in the art and can be used in the composition. They include, for example, photoactivated bleaches, or preformed organic peracids such as peroxycarboxylic acids or their salts, or peroxysulfonic acids or their salts. A suitable organic peracid is phthaloyl imido peroxycaproic acid. If used, the composition may typically contain such bleaches, and in some examples zinc phthalocyanine sulfonate, at about 0.025% to about 1.25% by weight of the composition.

[0110] Brightening Agents Optical brightening agents or other brightening or whitening agents may be incorporated at a level of from about 0.01% to about 1.2% by weight of the composition.

[0111] Commercially available optical brighteners that may be used herein can be divided into subgroups that include, but are not necessarily limited to, derivatives of stilbenes, pyrazolines, coumarins, benzoxazoles, carboxylic acids, methinecyanines, dibenzothiphene-5,5-dioxides, azoles, 5- and 6-membered heterocycles, and various other agents.

[0112] In some examples, the optical brightener is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (brightener 15, commercially available under the trade name Tinopal AMS-GX by Ciba Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-bis-hydroxyethyl)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal UNPA-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal 5BM-GX by Ciba-Geigy Corporation), or disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal 5BM-GX by Ciba-Geigy Corporation). More preferably, the optical brightener is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate.

[0113] The whitening agent may be added in particulate form or as a premix with a suitable solvent, such as a non-ionic surfactant, monoethanolamine, propanediol.

[0114] Fabric hueing agents. The composition may include a fabric hueing agent (sometimes referred to as a tinting agent, bluing agent, or whitening agent). Typically, the hueing agent imparts a blue or blue-purple hue to the fabric. The hueing agents may be used either alone or in combination to create a particular hue shade and / or tint different types of fabric. This may be achieved, for example, by mixing a red and a green-blue dye to produce a blue or purple shade. The hueing agent may be selected from any known chemical class of dyes, including, but not limited to, acridines, anthraquinones (including polycyclic quinones), azines, azos including premetallized azos (e.g., monoazos, diazos, trisazos, tetrakisazos, polyazos), benzodifuranes and benzodifuranones, carotenoids, coumarins, cyanines, diazahemicyanines, diphenylmethanes, formazans, hemicyanines, indigoids, methanes, naphthalimides, naphthoquinones, nitro and nitroso, oxazines, phthalocyanines, pyrazoles, stilbenes, styryls, triarylmethanes, triphenylmethanes, xanthenes, and mixtures thereof.

[0115] Encapsulation. The composition may include an encapsulating agent. The encapsulating agent may include a core and a shell having an inner surface and an outer surface, the shell encapsulating the core.

[0116] In certain embodiments, the encapsulant comprises a core and a shell, the core comprises a material selected from perfumes, brighteners, dyes, insect repellents, silicones, waxes, fragrances, vitamins, fabric softeners, skin care agents, such as paraffin, enzymes, antimicrobials, bleaches, sensates, or mixtures thereof, and the shell comprises a material selected from polyethylene, polyamides, polyvinyl alcohols, optionally containing other co-monomers, polystyrene, polyisoprene, polycarbonates, polyesters, polyacrylates, polyolefins, polysaccharides, such as alginates and / or chitosan, gelatin, shellac, epoxy resins, vinyl polymers, water insoluble inorganic materials, silicones, amino resins, or mixtures thereof. In some embodiments where the shell comprises an aminoplast, the aminoplast comprises a polyurea, polyurethane, and / or polyurea urethane. The polyurea may comprise polyoxymethylene urea and / or melamine formaldehyde.

[0117] Other Ingredients. The composition may further comprise a silicate. Suitable silicates may include, for example, sodium silicate, sodium disilicate, sodium metasilicate, crystalline phyllosilicates, or combinations thereof. In some embodiments, the silicate may be present at a concentration of about 1% to about 20% by weight, based on the total weight of the composition.

[0118] The composition may further comprise conventional detergent ingredients such as fabric conditioners, clays, suds boosters, suds suppressors, corrosion inhibitors, soil suspension agents, soil anti-redeposition agents, dyes, germicides, anti-tarnish agents, optical brighteners, or fragrances.

[0119] The composition optionally comprises a saturated or unsaturated fatty acid, preferably a saturated or unsaturated C 12 ~C 24fatty acids; deposition aids such as polysaccharides, cellulose polymers, polydiallyldimethylammonium halides (DADMAC) and copolymers of DADMAC in random or block configurations with vinylpyrrolidone, acrylamide, imidazole, imidazolinium halides, and mixtures thereof, cationic guar gum, cationic cellulose, cationic starch, cationic polyacylamide, or combinations thereof. When present, the fatty acids and / or deposition aids may each be present at 0.1% to 10% by weight based on the total weight of the composition.

[0120] The composition may optionally include silicone or fatty acid based suds suppressors; hue dyes, calcium and magnesium cations, visual signaling components, antifoaming agents (0.001% to about 4.0% by weight, based on the total weight of the composition), and / or structurants / thickeners (0.01% to 5% by weight, based on the total weight of the composition) selected from the group consisting of di- and triglycerides, ethylene glycol distearate, microcrystalline cellulose, microfiber cellulose, biopolymers, xanthan gum, gellan gum, and mixtures thereof.

[0121] additive composition The additive composition of the present disclosure may include additional auxiliary components. Such auxiliary agents may provide additional treatment benefits to the target fabric and / or they may act as stabilizing or processing aids to the composition. Suitable auxiliary agents may include chelating agents, fragrances, structuring agents, chlorine scavengers, malodor reducing materials, organic solvents, or mixtures thereof. EXAMPLES

[0122] Bath towel swatches with strong malodors were treated with four different products that were added through the wash (TTW). Product 1: Liquid laundry detergent (Tide® Original Scent, Procter & Gamble) and polyethylene glycol (PEG) beads (100% PEG). Product 2: Liquid laundry detergent and beads containing 99.99% by weight PEG and 0.01% by weight Bacillus spores. Product 3: Liquid laundry detergent and Saccharomyces supernatant (supplied as Saccharomyces cerevisiae fermentation filtrate under the product name DeoPlex® N17733 by Carrubba Incorporated, Milford, CT 06460, USA). Product 4: Liquid laundry detergent and beads containing 99.99% by weight PEG and 0.01% by weight Bacillus spores and Saccharomyces supernatant.

[0123] The swatches were machine washed, dried, and rebloomed after 24 and 48 hours and evaluated for malodor. Treatment was done on the wash cycle, using 2360 ppm laundry detergent, 5.5 x 10 6 Colony forming units (CFU) and 1867 ppm of Saccharomyces supernatant were used. The towel swatches were air-dried after washing and then individually placed in sealed sterile plastic cups for malodor evaluation overnight for 24 hours. Prior to olfactory evaluation of malodor, the towel swatches in the plastic cups were rebloomed by spraying with deionized water equivalent to 33% of the weight of the fabric in the cup and then incubated at 37°C for 1 hour. The towel swatches in the plastic cups were then equilibrated at room temperature before evaluation. Volunteer evaluators were selected from those familiar with bath towel malodor and asked to rank the bath towels from low to high malodor from each of the four sets of cups dedicated to them. Eight people evaluated 32 samples. After evaluation, the towel swatches were left in the cups for another 24 hours at ambient temperature, before a second evaluation was performed at 48 hours. The towel swatches were rebloomed a second time by spraying them with deionized water equivalent to 33% of the weight of the fabric in the cup and then incubated at 37° C. for 1 hour, then allowed to equilibrate at room temperature before evaluation.

[0124] The table below shows the malodor evaluation results, ranked numerically from low malodor (1) to high malodor (4) for the four products. As shown, towel swatches washed with product 4 (in accordance with the present invention) show synergistic malodor reduction at 24 and 48 hours.

[0125] [Table 1]

[0126] Dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise indicated, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40 mm" is intended to mean "about 40 mm."

Claims

1. A method for treating a surface, comprising: a) bacterial spores; b) Saccharomyces supernatant; and The method includes the step of treating with.

2. 2. The method of claim 1, wherein the bacterial spore comprises a Bacillus spore.

3. 3. The method of claim 2, wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, and mixtures thereof.

4. The method according to claim 1 or 2, wherein the surface is a soft surface or a hard surface.

5. The method of claim 1 or 2, wherein the surface is a fabric.

6. 6. The method of claim 5, wherein the treatment step is a laundry process including wash, rinse and dry cycles, and the bacterial spores and / or the Saccharomyces supernatant are delivered to the fabric separately or together from a cleaning composition and / or an additive composition.

7. The method of claim 6, wherein the Saccharomyces supernatant is supplied to the wash and the bacterial spores are supplied to the wash in the form of beads.

8. 7. The method of claim 6, wherein the Saccharomyces supernatant is provided to the washing and the bacterial spores are provided to the rinsing or drying step.

9. 9. The method of claim 8, wherein the treating step comprises adding the Saccharomyces supernatant to form an aqueous liquid containing 0.01% to 10% by weight of the Saccharomyces supernatant.

10. The bacterial spores are 1×10 per kilogram of fabric. ２ CFU~1×10 ７ The method of claim 8, wherein the composition is added from a cleaning composition or an additive composition at a concentration of CFU.

11. A product comprising: a) Bacterial spores and b) Saccharomyces supernatant; and Including the product.

12. The product of claim 11 , wherein the product comprises a cleaning composition and / or an additive composition.

13. The cleaning composition and / or the additive composition may be 1×10 ２ ~1×10 ９ 13. The product of claim 12 comprising CFU / g bacterial spores and 0.05% to 10% Saccharomyces supernatant by weight of the composition.

14. 14. The product of claim 12 or 13, wherein the cleaning composition is in the form of a liquid, solid, or unit dose form, and the additive composition is in the form of a cleaning additive, a rinse additive, or a drying additive.

15. 14. The product of claim 12 or 13, wherein the cleaning composition comprises a surfactant system comprising an ionic surfactant and a non-ionic surfactant.

16. The product described in claim 12, wherein the additive composition comprises the bacterial spores and polyethylene glycol and is in the form of beads.

17. The product is a laundry composition, the laundry composition comprising: a) from 5% to 55% by weight of the composition of an ionic surfactant; b) 5% to 50% by weight of the composition of a nonionic surfactant; c) 1×10 of the composition ２ ~1×10 ９ CFU / g of bacterial spores; d) 0.05% to 10% by weight of the composition of Saccharomyces supernatant; e) adjuvants including one or more of additional enzymes, peroxy compounds, bleach activators, anti-redeposition agents, neutralizing agents, optical brighteners, suds suppressors, chelating agents, bittering agents, dye transfer inhibitors, stain release agents, water softeners, electrolytes, pH adjusters, graying inhibitors, anti-wrinkle ingredients, bleaching agents, colorants, fragrances, processing aids, and mixtures thereof; The article of manufacture of claim 11 comprising:

18. 12. Use of the method of treatment of claim 1 or the product of claim 11 to provide sustained malodour relief to a substrate.