Decontamination composition and its uses

Abstract

A decontamination composition comprising chloroperoxidase, which can rapidly decompose chemical weapons, i.e., mustard gas (HD), lewisite (L) and VX, and the decontamination products of mustard gas and lewisite are non-toxic, thereby effectively preventing the harm of chemical weapons to the environment and organisms, and reducing the degree of harm of the products to soil, vegetation and organisms compared with conventional decontamination agents.

Description

[Technical field]

[0001] This application is based on and claims priority to an application having CN Application No. 202111032492.9 filed on September 3, 2021. The disclosure of the CN application is incorporated herein in its entirety.

[0002] Technical Field This application relates to the field of environmental decontamination and decontamination of biological organisms, and in particular to compositions and applications thereof that are particularly suitable for the decontamination of chemical poisons. [Background technology]

[0003] Chemical toxicants, commonly referred to as chemical weapons (CWAs), are a wide variety of chemicals used for warfare that are highly toxic and capable of poisoning or killing enemy humans, animals, and plants on a large scale. These include nerve agents, blister agents, systemic poisons, incapacitating agents, irritants, and asphyxiants.

[0004] In order to reduce the harm of chemical poisons, decontamination agents must be used to remove the toxic effects, and decontamination agents have become a research hotspot in the field of chemical poison protection.

[0005] Commonly used decontamination agents include sodium hypochlorite, DS2 (decontamination solution 2) and reactive skin decontamination lotion (RSDL). However, these decontamination agents have many drawbacks. For example, sodium hypochlorite and DS2 are corrosive and not suitable for general daily use, and RSDL has a negative effect on skin trauma. Therefore, bio-friendly cleaning methods are still attracting a lot of attention. Summary of the Invention

[0006] Contents of the Invention The present invention aims to develop a new generation of efficient and safe protease decontamination system to combat the threats of chemical warfare and chemical terrorism. The efficiency and safety of the system have been verified by in vitro data and animal models, and can achieve rapid broad-spectrum decontamination within one minute, laying the foundation for the development of a new generation of human-friendly decontamination agents. Research into new broad-spectrum enzyme decontamination agents will promote the improvement of the capabilities of military equipment defense systems, the improvement of anti-terrorism systems, and the establishment of a national chemical safety system.

[0007] This study overcomes the shortcomings of common methods to degrade chemical toxicants (CWAs) by exploring chloroperoxidase (CPO)-catalyzed oxidative degradation of chemical toxicants and demonstrates that it is a milder and more effective alternative. - Under optimized conditions, such as pH, and co-solvents, CPO can effectively decompose other types of chemical poisons, including sulfur mustard gas (HD), Lewisite (L), Agent Yellow (HD+L), and VX, without producing any harmful products.

[0008] In one embodiment, the present invention provides a decontamination composition comprising chloroperoxidase.

[0009] In some embodiments, the chloroperoxidase is CPO (EC 1.11.1.10).

[0010] In some embodiments, the decontamination composition further comprises one or more selected from the group consisting of hydrogen peroxide, halide ions, buffers, and co-solvents.

[0011] In some embodiments, the decontamination composition further comprises a component or combination selected from the group consisting of: hydrogen peroxide; halide ions; buffers; co-solvents; hydrogen peroxide and halide ions; hydrogen peroxide and buffers; hydrogen peroxide and co-solvents; halide ions and buffers; halide ions and co-solvents; buffers and co-solvents; hydrogen peroxide, halide ions and buffers; hydrogen peroxide, halide ions and co-solvents; hydrogen peroxide, buffers and co-solvents; halide ions, buffers and co-solvents; and hydrogen peroxide, halide ions, buffers and co-solvents.

[0012] In some embodiments, the decontamination composition comprises chloroperoxidase, hydrogen peroxide, a halide ion, a buffer, and a co-solvent.

[0013] In some embodiments, the decontamination composition is characterized by one or more of the following: (1) the halide ion is selected from the group consisting of fluoride ion, chloride ion, and bromide ion; preferably chloride ion; (2) The buffer is selected from the group consisting of a phosphate buffer, a borate buffer, and a citrate buffer; preferably a phosphate buffer, such as a KH2PO4 buffer; (3) The co-solvent is selected from alcohols, such as m-tert-butyl alcohol.

[0014] In some embodiments, the decontamination composition is characterized by one or more of the following: (1) The chloroperoxidase has a concentration of nanomolar to millimolar; for example, 1 nM to 100 mM, preferably 20 nM to 10 mM; (2) the hydrogen peroxide has a concentration of 0 to 50 mM; (3) the halide ion has a concentration of 0 to 0.5 M; (4) The buffer has a pH of 2.0 to 5.0; (5) The co-solvent has a concentration of 1 to 10% (v / v).

[0015] In some embodiments, the chloroperoxidase is 1 nM to 50 mM, 1 nM to 20 mM, 1 nM to 10 mM, 1 nM to 1 mM, 1 nM to 500 nM, 1 nM to 200 nM, 1 nM to 100 nM, 1 nM to 50 nM, 1 nM to 30 nM, 1 nM to 20 nM, 1 nM to 10 nM, 10 nM to 100 mM, 10 nM to 50 mM, 10 nM to 20 mM, 10 nM to 10 mM, 10 nM to 1 mM, 10 nM to 500 nM, 10 nM to 200 nM, 10 nM to 10 0nM,10nM~50nM,10nM~30nM,10nM~20nM,20nM~100mM,20nM~50mM,20nM~20mM,20nM~10mM,20nM~1mM,20nM~500nM,20nM~200n M, 20nM~100nM, 20nM~50nM, 20nM~30nM, 30nM~100mM, 30nM~50mM, 30nM~20mM, 30nM~10mM, 30nM~1mM, 30nM~500nM, 30nM~200nM , 30nM~100nM, 30nM~50nM, 50nM~100mM, 50nM~50mM, 50nM~20mM, 50nM~10mM, 50nM~1mM, 50nM~500nM, 50nM~200nM, 50nM~100n M,100nM~100mM,100nM~50mM,100nM~20mM,100nM~10mM,100nM~1mM,100nM~500nM,100nM~200nM,200nM~100mM,200nM~50mM, The concentration is 200nM to 20mM, 200nM to 10mM, 200nM to 1mM, 200nM to 500nM, 500nM to 100mM, 500nM to 50mM, 500nM to 20mM, 500nM to 10mM, 500nM to 1mM, 1mM to 100mM, 1mM to 50mM, 1mM to 20mM, 1mM to 10mM, 10mM to 100mM, 10mM to 50mM, 10mM to 20mM, 20mM to 100mM, 20mM to 50mM, or 50mM to 100mM.

[0016] In some embodiments, hydrogen peroxide is 50 nM to 50 mM, 50 nM to 20 mM, 50 nM to 10 mM, 50 nM to 5 mM, 50 nM to 1 mM, 50 nM to 500 nM, 50 nM to 200 nM, 50 nM to 100 nM, 100 nM to 50 mM, 100 nM to 20 mM, 100 nM to 10 mM, 100 nM to 5 mM, 100 nM to 1 mM, 100 nM to 500 nM, 100 nM to 200 nM, 200 nM to 50 mM, 200 nM to 20 mM 50mM, 200nM to 10mM, 200nM to 5mM, 200nM to 1mM, 200nM to 500nM, 500nM to 50mM, 500nM to 20mM, 500nM to 10mM, 500nM to 5mM, 500nM to 1mM, 1mM to 50mM, 1mM to 20mM, 1mM to 10mM, 1mM to 5mM, 5mM to 50mM, 5mM to 20mM, 5mM to 10mM, 10mM to 50mM, 10mM to 20mM, or 20mM to 50mM.

[0017] In some embodiments, the halide ion is 50 nM to 0.5 M, 100 nM to 0.5 M, 200 nM to 0.5 M, 500 nM to 0.5 M, 1 mM to 0.5 M, 10 mM to 0.5 M, 20 mM to 0.5 M, 30 mM to 0.5 M, 50 mM to 0.5 M, 0.1 M to 0.5 M, 0.2 M to 0.5 M, 50 nM to 0.2 M, 100 nM to 0.2 M, 200 nM to 0.2 M, 500 nM M~0.2M, 1mM~0.2M, 10mM~0.2M, 20mM~0.2M, 30mM~0.2M, 50mM~0.2M, 0.1M~0.2M, 50nM~0.1M, 100nM~0.1M, 200nM~0.1M, 500nM~0.1M, 1mM~0.1M, 10mM~0.1M, 20mM~0.1M, 30mM~0.1M, 50mM~0.1M, 50nM~50mM, 100nM~ 50mM, 200nM~50mM, 500nM~50mM, 1mM~50mM, 10mM~50mM, 20mM~50mM, 30mM~50mM, 50nM~30mM, 100nM~30mM , 200nM~30mM, 500nM~30mM, 1mM~30mM, 10mM~30mM, 20mM~30mM, 50nM~20mM, 100nM~20mM, 200nM~20mM, 500 The concentration is nM to 20 mM, 1 mM to 20 mM, 10 mM to 20 mM, 50 nM to 10 mM, 100 nM to 10 mM, 200 nM to 10 mM, 500 nM to 10 mM, 1 mM to 10 mM, 50 nM to 1 mM, 100 nM to 1 mM, 200 nM to 1 mM, 500 nM to 1 mM, 50 nM to 500 nM, 100 nM to 500 nM, 200 nM to 500 nM, or 100 nM to 200 nM.

[0018] In some embodiments, the buffer has a pH of 2.0-2.5, 2.0-3.0, 2.0-3.5, 2.0-4.0, 2.0-4.5, 2.0-5.0, 2.5-3.0, 2.5-3.5, 2.5-4.0, 2.5-4.5, 2.5-5.0, 3.0-3.5, 3.0-4.0, 3.0-4.5, 3.0-5.0, 3.5-4.0, 3.5-4.5, 3.5-5.0, 4.0-4.5, or 4.5-5.0.

[0019] In some embodiments, the co-solvent is 1-2% (v / v), 1-3% (v / v), 1-4% (v / v), 1-5% (v / v), 1-6% (v / v), 1-7% (v / v), 1-8% (v / v), 1-9% (v / v), 1-10% (v / v), 2-3% (v / v), 2 ~4%(v / v), 2~5%(v / v), 2~6%(v / v), 2~7%(v / v), 2~8%(v / v), 2~9%(v / v), 2~ 10%(v / v), 3~4%(v / v), 3~5%(v / v), 3~6%(v / v), 3~7%(v / v), 3~8%(v / v),3~ 9%(v / v),3~10%(v / v),4~5%(v / v),4~6%(v / v),4~7%(v / v),4~8%(v / v),4~ 9%(v / v),4~10%(v / v),5~6%(v / v),5~7%(v / v),5~8%(v / v),5~9%(v / v),5~ 10%(v / v), 6~7%(v / v), 6~8%(v / v), 6~9%(v / v), 6~10%(v / v), 7~8%(v / v), 7 It has a concentration of ~9% (v / v), 7-10% (v / v), 8-9% (v / v), 8-10% (v / v), or 9-10% (v / v).

[0020] In another aspect, the present invention provides a decontamination preparation comprising a decontamination composition according to any one of the items of the first aspect.

[0021] In some embodiments, the hydrogen peroxide and other components of the decontamination composition are located in the same formulation unit.

[0022] In some embodiments, the hydrogen peroxide and other components of the decontamination composition are located in different preparation units.

[0023] In some embodiments, the decontamination formulation is a decontamination solution.

[0024] In another aspect, the present invention provides a protective equipment comprising a decontamination composition according to any one of the items of the first aspect or a decontamination formulation according to any one of the items of the second aspect.

[0025] In another aspect, the present invention provides the use of a decontamination composition according to any one of the items of the first aspect, a decontamination formulation according to any one of the items of the second aspect, or a protective equipment according to any one of the items of the third aspect in decontaminating a chemical toxicant.

[0026] In some embodiments, the chemical toxin is a nerve agent or a blister agent.

[0027] In some embodiments, the nerve agent is an organophosphate agent, for example, selected from the group consisting of sarin, tabun, soman, and VX.

[0028] In some embodiments, the blister agent is mustard gas, lewisite, nitrogen mustard, or a combination thereof, such as a combination of lewisite and mustard gas.

[0029] In another aspect, the present invention provides a method for decontaminating a chemical toxicant, comprising the steps of: (1) Rapidly mixing the components of the decontamination composition according to any one of the items in the first aspect or the decontamination formulation according to any one of the items in the second aspect to obtain a mixed system; (2) Pouring, spraying, or contacting the mixture into areas where chemical toxicants may leak, such as water, soil, or biological surfaces.

[0030] In some embodiments, the chemical toxin is a nerve agent or a blister agent.

[0031] In some embodiments, the nerve agent is an organophosphate agent, for example, selected from the group consisting of sarin, tabun, soman, and VX.

[0032] In some embodiments, the blister agent is mustard gas, lewisite, nitrogen mustard, or a combination thereof, such as a combination of lewisite and mustard gas. Effect of the Invention

[0033] Beneficial Effects of the Invention The decontamination formulation of the present invention is aimed at mustard gas, lewisite or agent yellow, and the decontamination product is non-toxic and non-irritating to living organisms. Thorough decontamination is achieved within one minute. Enzyme consumption is at nanomolar level. It is efficient, safe and environmentally friendly, thereby minimizing the subsequent impact of spill accidents. [Brief description of the drawings]

[0034] The drawings described in this specification are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their description are used to explain the present invention and do not constitute an undue limitation of the present invention. In the accompanying drawings:

[0035] [Figure 1] (a) Photographs taken under a light microscope showing a comparison of the effects on survival of zebrafish embryos of the group using the decontamination formulation compared to the group using only hydrogen peroxide, the non-decontaminated mustard gas control group, and the group using a conventional decontamination agent; (b) Embryo survival rate.

[0036] [Diagram 2] (a) Decontamination effects against the chemical toxicants mustard gas, lewisite, and VX in the group using the decontamination formulation, the group using hydrogen peroxide only, and the chemical toxicant control group; (b) In the case of lewisite, the response of zebrafish larvae to the decontamination formulation; (c) In the case of Agent Yellow, the response of zebrafish larvae to the decontamination formulation (the group using the decontamination formulation of the present invention, and the chemical toxicant control group).

[0037] [Diagram 3] (a) Michaelis-Menten analysis of the enzymatic oxidation of mustard gas; (b) kinetics of the decomposition of mustard gas and the formation of mustard sulfoxide; (c) mass and NMR spectra of the products of the enzymatic degradation of mustard gas; (d) proposed mechanism for the decomposition of mustard gas.

[0038] [Figure 4] FIG. 2 is an exploration diagram of optimal conditions for each formula of the decontamination formulation of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0039] Specific Model for Carrying Out the Invention The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the present invention, its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without inventive ideas belong to the protection scope of the present invention.

[0040] Chloroperoxidase (CPO, EC 1.11.1.10) is a multifunctional glycoprotein secreted by the marine fungus Caldariomyces fumago and is one of the most versatile catalytic heme enzymes. However, CPO has a broad substrate selectivity and is therefore less efficient at degrading HD.

[0041] Zebrafish (Danio rerio) embryos are often used as a vertebrate model for environmental and animal physiological monitoring because of their advantages in terms of chemical permeability, short disease latency, sensitivity to chemical treatment, and ease of observation and manipulation. Furthermore, the operating conditions of CPO do not interfere with the developmental conditions of zebrafish, making it possible to establish a new environmental model combining enzymes and zebrafish embryos to detect the decontamination efficiency. EXAMPLES

[0042] Example 1: All procedures were performed in a well-ventilated fume hood at 25 ± 5 °C, and freshly prepared H2O2 stock solution was used in each experiment. Zebrafish embryos were collected from the egg-laying apparatus, rinsed with embryo medium E3, and split into 24-well plates, 10 embryos per well. A pipette was used to carefully remove the culture medium from the wells of the culture plate, and then 1 ml of the test solution was added to the culture well within 4 h after fertilization. The culture plate was placed flat and incubated at 27 ± 1 °C, and embryo mortality was recorded daily for 3 days. Data were subsequently expressed as the mean ± standard deviation of the results of three independent parallel experiments. The CPO reaction conditions were optimized by using blank embryo culture medium E3 as a negative control, and specific solutions of 0.1-5% (vol%) TBA, 1-100 mM KH2PO4, 0.1-50 mM KCl, and 0.05-100 μM HD (pH 3.0-7.0) were added to the embryo culture medium. The toxicity of HD and its degradation products was evaluated by establishing a system in which 0.1 mM HD was degraded with 20 nM CPO and 0.2 mM H2O2 at pH 4.5 for 5 min.

[0043] As shown in Figure 1, all embryos treated with 0.1 mM HD died within 24 hours, while the survival rates of CPO-treated and negative control embryos were 87±8% and 93±6%, respectively. Results are expressed as mean±standard deviation, n=3. In the last two cases, embryos and larvae did not show malformations or morphological changes. However, embryos exposed to 0.5% sodium hypochlorite were immediately damaged.

[0044] Example 2: HD stock solution (0.1 mM), obtained by dissolving pure HD in TBA, was added to a buffer solution containing 0.1 M KH2PO4, 0.5 M KCl, 0.022 μM CPO, and 5 mM H2O2 at pH 2.75 to obtain a HD stock solution with a final concentration of 1 mM. In a separate experiment, H2O2 was reacted with HD in the absence of CPO to confirm the important role of the enzyme in this process. A control sample was separately prepared by decomposing HD in a buffer solution without CPO or H2O2. After reacting for 1 min, the organic layer was quenched and separated with an equal volume of CH2Cl2, then dried over anhydrous Na2SO4, subsequently transferred to a gas chromatography vial, stored at 20 °C, and finally analyzed by gas chromatography mass spectrometry. A similar method was used to study the degradation of 1 mM L and VX under the conditions of a CPO concentration of 0.220 μM and reaction times of 1 and 5 min, respectively. As can be seen from Fig. 2a, the complete degradation of HD and LVX was rapidly achieved by CPO-catalyzed oxidation at nanomolar enzyme consumption and 5 mM H2O2 concentration, revealing that CPO can simultaneously promote the degradation of various CWAs. At 1 mM H2O2 concentration, HD and L were completely consumed within 1 min, 1 mM HD within 40 s, and 1 mM VX within 5 min. Under the condition of 5 mM H2O2 concentration and no CPO, the remaining amounts of HD, L, and VX obtained after 1 h, 1 min, and 5 min of reaction, respectively, were 83.85 ± 5.01%, 78.36 ± 11.56%, and 35.72 ± 0.77%. Thus, except for the degradation rate of VX reaching 60% within 5 min, the non-catalyzed reaction between chemical toxicants and H2O2 was relatively slow.

[0045] Example 3: Zebrafish larvae at 3 days post-fertilization were used to evaluate the degradation efficiency of L and Agent Yellow (HD+L) by CPO. Zebrafish larvae were split and added to a 24-well plate with 6 larvae per well. 0.1 mM L solutions, CPO and H2O2 were added to modified embryonic E3 medium with final concentrations of 20 nM L, 0.2 mM CPO and H2O2, respectively, and the reaction was continued for 5 min. Additional experiments were performed with samples containing only 0.1 mM L and 0.1 mM L + 0.2 mM H2O2 in embryonic E3 medium. In addition, 0.5% sodium hypochlorite was tested as an oxidizing agent. The degradation of a mixture of 0.1 mM HD and 0.1 mM L was studied with 20 nM CPO and 0.5 mM H2O2, respectively. In modified embryonic E3 medium containing TBA (0.1%) and KH2PO4 (1-10 mM), the optimal reaction conditions for HD were determined by adjusting the pH to 4.5 with HCl. In the CPO system, L could be effectively decomposed, as shown in Figure 2b-2c, but the oxidation rate without CPO was not sufficient and could produce toxic light-absorbing products. In the presence of 0.5% sodium hypochlorite, zebrafish larvae were immediately damaged, died within a few minutes, and decomposed after 5 minutes. With 1 mM L, cardiac arrest was observed within 20 minutes, and the bodies of dead larvae had a twisted and corroded appearance. Larvae treated with 0.1 mM L and 0.2 mM H2O2 died within 20 minutes, and their skin was blackened. Similar results were obtained when Agent Yellow was decomposed with 0.1 mM HD + 0.1 mM L. That is, CPO can effectively decompose HD and L and can be used as a practical chemical warfare decontamination agent to decompose Agent Yellow.

[0046] Example 4: The optimal conditions were set as follows: 0.1 M KH2PO4, 0.5 M KCl, pH 2.75, 5% TBA, 5 mM H2O2, 20 nM CPO, temperature 25 ± 2 °C, total volume 7 ml. Under the optimal reaction conditions, the decomposition rate of HD catalyzed by CPO was monitored. Every 5 or 10 seconds, 1 ml of reaction mixture was taken out and immediately mixed with 1 ml of CH2Cl2 while stirring with a magnetic stirrer for 1 min to obtain a sample for later detection. Michaelis-Menten analysis was used to measure the affinity of CPO for HD, and GraphPad Prism 5 was used for the graph analysis. As shown in Figure 3, the Km, Vmax, and kcat values ​​were 0.17 mM, 0.06 mM s, respectively. -1 (R 2 =0.935), 2717M -1 s -1 For extremely efficient enzymes, the reaction rate is 10 7 M -1 s -1 For ultra-efficient enzymes, the kcat / Km ratio is 10 8 ~10 10 M -1 s -1 The corresponding CPO value was 1.58 × 10 7 M -1 s -1 This indicates that CPO has the ability to effectively promote HD oxidation.

[0047] In addition to those described herein, various modifications of the present invention will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to be included within the scope of the appended claims. Each document cited in this application, including all patents, patent applications, journal articles, books, and any other publications, is incorporated herein by reference in its entirety.

Claims

1. A decontamination composition comprising chloroperoxidase; Preferably, the decontamination composition, wherein the chloroperoxidase is CPO (EC 1.11.1.10).

2. further comprising one or more selected from the group consisting of hydrogen peroxide, halide ions, buffers, and co-solvents; 10. The decontamination composition of claim 1, wherein the decontamination composition further comprises a component or combination selected from the group consisting of: hydrogen peroxide; halide ions; buffer; co-solvent; hydrogen peroxide and halide ions; hydrogen peroxide and buffer; hydrogen peroxide and co-solvent; halide ions and buffer; halide ions and co-solvent; buffer and co-solvent; hydrogen peroxide, halide ions, and buffer; hydrogen peroxide, halide ions, and co-solvent; hydrogen peroxide, buffer, and co-solvent; halide ions, buffer, and co-solvent; and hydrogen peroxide, halide ions, buffer, and co-solvent.

3. the below described: (1) The halide ion is selected from the group consisting of fluoride ion, chloride ion, and bromide ion; preferably chloride ion; (2) The buffer solution is selected from the group consisting of a phosphate buffer solution, a borate buffer solution, and a citrate buffer solution; preferably a phosphate buffer solution, such as KH ２ P.O. ４ buffer solutions, etc.; (3) The co-solvent is selected from alcohols such as tert-butyl alcohol; 3. The decontamination composition of claim 2, characterized by one or more of the following:

4. the below described: (1) The chloroperoxidase has a concentration of nanomolar to millimolar levels, for example, 1 nM to 100 mM, preferably 20 nM to 10 mM; (2) the hydrogen peroxide has a concentration of 0 to 50 mM; (3) the halide ions have a concentration of 0 to 0.5M; (4) The buffer has a pH of 2.0 to 5.0; (5) The co-solvent has a concentration of 1 to 10% (v / v).

3. The decontamination composition of claim 2, characterized by one or more of the following:

5. A decontamination formulation comprising the decontamination composition of any one of claims 1 to 4, comprising: Preferably, the hydrogen peroxide and other components in the decontamination composition are located in the same preparation unit; Preferably, the hydrogen peroxide and other components in the decontamination composition are located in different preparation units; Preferably, the decontamination formulation is a decontamination solution.

6. A protective device comprising the decontamination composition of any one of claims 1 to 4.

7. Use of a decontamination composition according to any one of claims 1 to 4 in the decontamination of chemical toxicants, comprising: Preferably, the chemical toxicant is a nerve agent or a blister agent; Preferably, the nerve agent is an organophosphate agent, e.g., selected from the group consisting of sarin, tabun, soman, and VX; Preferably, the blister agent is mustard gas, lewisite, nitrogen mustard or a combination thereof, for example a combination of lewisite and mustard gas.

8. 1. A method for decontaminating a chemical toxicant, comprising the steps of: (1) Rapidly mixing the components of the decontamination composition according to any one of claims 1 to 4 to obtain a mixed system; (2) Pouring, spraying, or contacting the mixture with the area where the chemical toxicant is likely to leak, such as water, soil, or biological surfaces; Including, Preferably, the chemical toxicant is a nerve agent or a blister agent; Preferably, the nerve agent is an organophosphate agent, e.g., selected from the group consisting of sarin, tabun, soman, and VX; Preferably, the blister agent is mustard gas, lewisite, nitrogen mustard, or a combination thereof, for example, a combination of lewisite and mustard gas.

Images