Skin external preparation

Abstract

To provide a skin external preparation that exhibits a synergistically high anti-aging effect through the combined application of niacinamide, bakuchiol, and Sanguisorba officinalis extract.SOLUTION: A skin external preparation is provided that contains niacinamide as a component (A), bakuchiol as a component (B), and Sanguisorba officinalis extract as a component (C).SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an external skin preparation containing niacinamide, bactiol and a Sanguisorba officinalis extract. [Background technology]

[0002] A skin aging prevention cosmetic containing niacinamide is known (Patent Document 1: JP-A-10-130135). It is also known to incorporate bactiol into a cosmetic (Patent Document 2: JP-A-11-71231). It is also known to incorporate a Sanguisorba officinalis extract into a cosmetic (Patent Document 3: JP-A-2001-348335).

[0003] Many studies have been conducted on combining ingredients into topical skin preparations. However, the effects of the combination are unpredictable, as the effects are not synergistically improved by simply using the ingredients together, but are additively improved or offset, and there is a great need for combinations of ingredients that provide greater effects in smaller doses. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 10-130135 [Patent Document 2] Japanese Patent Application Publication No. 11-71231 [Patent Document 3] JP 2001-348335 A Summary of the Invention [Problem to be solved by the invention]

[0005] The present invention aims to provide an external skin preparation which exerts a synergistically high anti-aging effect by using niacinamide, bactiol and a Sanguisorba officinalis extract in combination. [Means for solving the problem]

[0006] The means for solving the problems of the present invention are as follows. A skin topical preparation containing component (A) niacinamide, component (B) bakuchiol, and component (C) Sanguisorba officinalis extract. Effect of the Invention

[0007] The skin external preparation of the present invention exhibits a synergistic anti-aging effect by using specific ingredients in combination.

[0008] The topical skin preparation of the present invention exhibits a synergistic wrinkle improving effect and / or skin elasticity improving effect by using specific ingredients in combination. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] Hereinafter, an embodiment of the present invention will be described.

[0010] MMP3 is an abbreviation for matrix metalloproteinase 3, and is a factor involved in the decomposition of proteoglycans, collagen, and other extracellular matrices in the body.

[0011] HYAL2 is an abbreviation for hyaluronidase 2, a factor involved in the decomposition of hyaluronic acid.

[0012] MMP1 is an abbreviation for matrix metalloproteinase 1, and is a factor involved in the decomposition of collagen, aggrecan, and other extracellular matrices in the body.

[0013] The component (A) niacinamide used in the present invention is one of the water-soluble vitamins of group B, and is also called nicotinamide. As the niacinamide, any of commercially available products, products conforming to the food additive standards, products conforming to the quasi-drug raw materials standards, and products conforming to the Japanese Pharmacopoeia may be used.

[0014] The amount of niacinamide is preferably 0.01% by mass or more, more preferably 0.05% by mass or more, and more preferably 10% by mass or less, and more preferably 7% by mass or less, preferably 0.01% by mass or more and 10% by mass or less, and more preferably 0.05% by mass or more and 7% by mass or less, based on the total amount of the topical skin preparation.

[0015] Bactiol, the component (B) used in the present invention, is a component contained in the seeds of Psoralea corylifolia, which is classified into the genus Psoralea in the family Fabaceae, and is a compound with CAS number 10309-37-2.

[0016] Bactiol can be naturally derived or synthetic.

[0017] The amount of bactiol in the skin topical preparation is preferably 0.000001% by mass or more, more preferably 0.00001% by mass or more, and more preferably 5% by mass or less, and more preferably 2% by mass or less, and preferably 0.000001% by mass or more and 5% by mass or less, and more preferably 0.00001% by mass or more and 2% by mass or less.

[0018] The component (C) Sanguisorba officinalis extract used in the present invention is an extract of Sanguisorba officinalis L., which is classified into the genus Sanguisorba in the family Rosaceae.

[0019] The Sanguisorba officinalis extract used in the present invention may be one that is usually incorporated into cosmetics, skin external preparations, etc. In the present invention, an extract obtained from Sanguisorba officinalis organically cultivated in Yunosawa, Mashike-cho, Hokkaido, may also be used.

[0020] The parts of Sanguisorba that can be used are not particularly limited, but the roots and / or rhizomes are preferred.

[0021] The amount of Sanguisorba officinalis extract is preferably 0.0000001% by mass or more, more preferably 0.000001% by mass or more, and more preferably 5% by mass or less, and more preferably 1% by mass or less, preferably 0.0000001% by mass or more and 5% by mass or less, and more preferably 0.000001% by mass or more and 1% by mass or less, based on the total amount of the topical skin preparation.

[0022] Next, a preferred example of a method for cultivating Sanguinea batatas will be described.

[0023] [Yunosawa, Mashike-cho, Hokkaido] The area is located in the northwest of Hokkaido, in the southern part of the Rumoi Regional Development Bureau, and includes Mt. Shokanbetsu, which is 1,492 m above sea level. In the present invention, it is preferable to cultivate the plant in the Yunosawa district, which is at a high altitude in Mashike Town.

[0024] [Field preparation] Before planting, organic fertilizer is applied to improve the soil quality of the field. Specifically, as organic fertilizers, one or more selected from fermented chicken manure, oil cake, fermented oil cake, bone meal, fish meal, rice bran, fermented rice bran, leaf mold, bark compost, dolomite, slaked lime, calcium carbonate, and guano are used in combination.

[0025] Fermented chicken manure, oil cake, fermented oil cake, bone meal, fish meal, rice bran, fermented rice bran, leaf mold, bark compost, and iodine phosphate fertilizer are mainly used to supply the three major nutrients: nitrogen, phosphorus, and potassium. These fertilizers are used alone or in combination of two or more types. The amount of these fertilizers applied can be increased or decreased depending on the condition of the original soil.

[0026] Fertilization is preferably carried out at least 7 days before planting. If fertilization is carried out within 6 days before planting, the soil improvement effect of the fertilizer will be insufficient, causing fertilizer burn and poor initial growth. Organic fertilizer can be applied in furrows or over the entire surface, but over the entire surface is preferred from the viewpoint of work efficiency.

[0027] [Planting] It is recommended to divide the plant in September or October and thin it out as it grows.

[0028] [Development] More remarkable growth can be expected by applying additional fertilizer appropriately. Remove weeds as necessary and water when the soil is dry.

[0029] [harvest] The rhizomes are harvested from mid-September to October.

[0030] [extraction] When preparing the extract, it is used after drying. As the extraction solvent, water, lower alcohols such as methanol, ethanol, propanol, isopropanol, etc., polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, glycerin, etc., ethers such as ethyl ether, propyl ether, etc., esters such as ethyl acetate, butyl acetate, etc., ketones such as acetone, ethyl methyl ketone, etc., and polar organic solvents can be used, and one or more of these can be selected and used. In addition, physiological saline, phosphate buffer, phosphate buffered physiological saline, etc. may be used. The extract using the above solvent can be used as it is, but it may be concentrated, dried, and then dissolved again in water or a polar solvent, or it may be used after purification treatment such as bleaching, deodorization, desalting, etc., or fractionation by column chromatography, within a range that does not impair the skin physiological function improvement effect. In addition, the extract may be hydrolyzed using an acid, alkali, enzyme, etc. and used. In addition, for storage, it can be freeze-dried after purification treatment, and dissolved in a solvent when used. It can also be used by being encapsulated in vesicles such as liposomes, microcapsules, etc. The extraction process is not particularly limited as long as the soluble components contained in the extraction raw material can be dissolved in the extraction solvent, and can be carried out according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent that is 5 to 30 times the amount (mass ratio) of the extraction raw material, and the soluble components are extracted at room temperature or under reflux heating, and then the extraction residue is removed by filtration to obtain an extract. The solvent is distilled off from the obtained extract to obtain a paste-like concentrate, and the concentrate is further dried to obtain a dry product.

[0031] In addition to the above-mentioned components, the skin topical preparation of the present invention may contain optional components used in ordinary skin topical preparations to the extent that the effects of the present invention are not impaired.Specific examples of the optional components include oils, surfactants, thickeners, preservatives, fragrances, moisturizers, antioxidants, anti-inflammatory agents, antibacterial agents, etc.

[0032] The formulation of the external skin preparation of the present invention is not particularly limited, and may be any formulation such as water-based, oil-based, or emulsion-type.

[0033] The skin external preparation of the present invention can be prepared by a conventional method.

[0034] The skin external preparation of the present invention can be used in the form of, for example, a lotion, emulsion, or ointment.

[0035] The topical skin preparation of the present invention uses a combination of component (A) niacinamide, component (B) bactiol, and component (C) Sanguisorba officinalis extract to synergistically reduce the expression of MMP3, HYAL2, or MMP1 factors in dermal fibroblasts and inhibit the decomposition of collagen or hyaluronic acid, thereby exerting anti-aging effects such as improving wrinkles and skin elasticity. EXAMPLES

[0036] The present invention will be described in detail below with reference to examples, but the scope of the present invention is not limited thereto. The blend amounts are in mass % unless otherwise specified.

[0037] First, the method for preparing the Sanguisorba officinalis extract used as the sample will be described.

[0038] [Sanguar oleander extract 1] (1) From cultivation to harvesting We cultivated sanguinea pigs in an organic JAS certified field in Yunosawa, Mashike-cho, Hokkaido. In mid-May, one week before planting, we fertilized the field with organic chicken manure, organic rice bran, organic lime, organic guano, organic rapeseed cake, and calcium carbonate. Planting was done by dividing the plants into separate plants with 30cm spacing between plants and 90-100cm spacing between furrows. In late June, we applied additional fertilizer with organic rapeseed cake. Weeds were also removed as needed, and watering was carried out depending on the dryness. Flower buds were removed to encourage good root growth, and rhizomes were harvested from mid-September to October and washed with water or hot water. They were stored under dry conditions or freeze-dried. (2) Preparation of Sanguisorba officinalis extract The rhizome of Sanguisorba officinalis obtained in (1) was dried and crushed, and then immersed in 20 times the mass of 50% ethanol aqueous solution, filtered, the filtrate was collected, and the ethanol was distilled off. The obtained extract was dissolved in 50% butylene glycol aqueous solution to a concentration of 0.5% by mass as pure extract, and then filtered again to prepare Sanguisorba officinalis extract 1.

[0039] [Sanguar oleander extract 2] (1) Plant origin Dried rhizomes of Sanguisorba officinalis cultivated in China were used. (2) Preparation of Sanguisorba officinalis extract The rhizome of (1) was crushed and immersed in an ethanol solution, and the filtrate was evaporated to dryness. The filtrate was dissolved in purified water, and the solvent was removed from the filtrate by filtration. The filtrate was redissolved in an ethanol solution. The extract was adjusted to a pure content of 1.6% by mass with a 50% ethanol aqueous solution, and designated as sanguisorba extract 2.

[0040] [Test using human newborn skin fibroblasts] 1 × 10 human neonatal skin fibroblasts 5 The cells were seeded in a 24-well plate at 100 cells / well and cultured overnight in DMEM medium containing 0.5% FBS. The medium was replaced with one containing the components shown in Table 2 in the amounts shown, and incubated at 37°C, 5% CO 2The cells were cultured for 24 hours in an incubator. RNA was extracted from the collected cells using a commercially available RNA extraction kit (Quick Gene RNA Cultured Cell Kit S), and after cDNA synthesis, gene expression was confirmed by real-time PCR using the Cyber ​​Green method with the following primers. GAPDH was used as an internal standard. Primer information is shown in Table 1, and the measurement results are shown in Tables 2 and 3. The measurement results are shown as relative values, with the gene expression level when no components were added being set at 1.

[0041] In the examples and comparative examples, each component was dissolved in the medium so that the concentration of each component and the extract concentration (w / v%) of the extract became the values ​​shown in Tables 2 and 3.

[0042] The primer sequences used are shown in Table 1.

[0043] [Table 1]

[0044] [Table 2]

[0045] [Table 3]

[0046] As shown in Table 2, in Example 1, in which component (A) niacinamide, component (B) bactiol, and component (C) Sanguisorba officinalis extract were used in combination, the expression of MMP3 factor in dermal fibroblasts was synergistically reduced compared to Comparative Examples 2 to 4 in which each component was added alone, even though the concentrations of each component and the extracts were one-third of those in the Comparative Examples.

[0047] As shown in Table 3, in Example 2, in which component (A) niacinamide, component (B) bactiol, and component (C) Sanguisorba officinalis extract were used in combination, the expression of HYAL2 factor and MMP1 factor in dermal fibroblasts was synergistically reduced compared to Comparative Example 2, Comparative Example 3, and Comparative Example 5, in which each component was added alone, even though the concentrations of each component and the extract were one-third of those in the Comparative Examples.

[0048] [Example 3] Cosmetic essence (1) Squalane 10.0 (mass%) (2) Methylphenylpolysiloxane 4.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0.1 (5) Bactiol 0.2 (6) Sodium acrylate, sodium acryloyldimethyltaurate Copolymer / Isohexadecane / Polysorbate 80 2.0 (7) 1,3-butylene glycol 4.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water (balance to make up 100) (10) Carboxyvinyl polymer (1% by weight aqueous solution) 20.0 (11) Arginine (1% by weight aqueous solution) 20.0 (12) Niacinamide 3.0 (13) Sanguisorba officinalis extract 1 0.1 Manufacturing method: The oil phase components (1) to (5) are heated and dissolved at 80°C. Meanwhile, the water phase components (6) to (11) are heated and dissolved at 80°C. The oil phase components are added to this while stirring, and the mixture is uniformly emulsified using a homogenizer. After cooling, (12) and (13) are added in that order at 40°C, and mixed uniformly.

[0049] [Example 4] Cream (1) Squalane 10.0 (mass%) (2) Stearic acid 2.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0.1 (5) Cetanol 3.6 (6) Lipophilic glyceryl monostearate 2.0 (7) Bactiol 0.01 (8) Glycerin 10.0 (9) Methyl parahydroxybenzoate 0.1 (10) Arginine (20% by weight aqueous solution) 15.0 (11) Purified water (balance to make up 100%) (12) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0 (13) Niacinamide 6.0 (14) Sanguisorba officinalis extract 1 0.5 Manufacturing method: The oil phase components (1) to (7) are heated and dissolved at 80°C. Meanwhile, the water phase components (8) to (11) are heated and dissolved at 80°C. The oil phase components are added to this while stirring, and the mixture is uniformly emulsified using a homogenizer. After emulsification, (12) is added, cooling is started, and (13) and (14) are added at 40°C and mixed uniformly.

[0050] [Example 5] Lotion (1) Ethanol 15.0 (2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3 (3)Fragrance 0.1 (4) Bactiol 0.1 (5) Purified water (balance to make up 100%) (6) Citric acid 0.02 (7) Sodium citrate 0.1 (8) Glycerin 1.0 (9) Hydroxyethyl cellulose 0.1 (10) Niacinamide 4.0 (11) Sanguisorba officinalis extract 2 0.01 Manufacturing method: Dissolve (2)-(4) in (1). Add (5)-(11) in order, and then stir thoroughly to mix uniformly.

Claims

[Claim 1] A skin topical preparation containing component (A) niacinamide, component (B) bakuchiol, and component (C) Sanguisorba officinalis extract.

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