Bioprocessing system, and tubing and component management apparatus for bioprocessing system

Abstract

To satisfy the need for a tubing and component management system for a bioprocessing system that is ergonomically efficient, facilitates installation and setup, and / or assists in the inflation and deflation of the flexible bioprocessing bag.SOLUTION: A bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, and a locking mechanism configured to retain the base plate in the recess.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] FIELD OF THE DISCLOSURE Embodiments of the present invention relate generally to bioprocessing systems and methods, and more particularly, to piping and component management systems for bioprocessing systems. [Background technology]

[0002] A variety of vessels, devices, components, and unit operations are known for carrying out biochemical and / or biological processes and / or for manipulating the liquids and other products of such processes. To avoid the time, expense, and difficulty associated with sterilizing vessels used in processes to manufacture biological products, single-use or disposable bioreactor bags and single-use mixer bags have been used as such vessels. For example, biological materials (e.g., animal and plant cells), including mammalian, plant, or insect cells, and microbial cultures, can be processed using disposable or single-use mixers and bioreactors.

[0003] Increasingly, single-use or disposable containers are being used in the biopharmaceutical industry. Such containers can be flexible or collapsible plastic bags supported by an outer rigid structure such as a stainless steel shell or container. The use of sterilized disposable bags eliminates the time-consuming step of cleaning the container and reduces the chance of contamination. The bag can be positioned within the rigid container and filled with the desired fluid for mixing. Depending on the fluid being processed, the system can include several fluid lines and various sensors, probes, and ports that are coupled with the bag for monitoring, analysis, sampling, and fluid transfer. For example, multiple ports are typically positioned at the front of the bag and can be accessible through an opening in the side wall of the container, which provides connection locations for sensors, probes, and / or fluid sampling lines. Also, a collection port or drain line fitting is typically positioned at the bottom of the disposable bag and configured for insertion through an opening in the bottom of the container, and a collection line can be connected to the bag for collection and drainage of the bag after the bioprocessing is completed.

[0004] Typically, an agitator assembly located within the bag is used to mix the liquid. Existing agitators are either top-driven (having a shaft that extends downward into the bag and on which one or more impellers are mounted) or bottom-driven (having an impeller located at the bottom of the bag that is driven by a magnetic drive system or motor located outside the bag and / or vessel). Most magnetic agitator systems include a rotating magnetic drive head on the outside of the bag and a rotating magnetic agitator (also referred to as an "impeller" in this context) within the bag. Movement of the magnetic drive head allows for torque transfer, thereby allowing rotation of the magnetic agitator, causing the agitator to mix the liquid within the vessel. Magnetic coupling of the agitator inside the bag to a drive system or motor outside the bag and / or bioreactor vessel eliminates contamination issues, allows for a fully enclosed system, and prevents leaks. A magnetically coupled system can also eliminate the need to have a seal between the drive shaft and the vessel, since there is no need to penetrate the wall of the bioreactor vessel to mechanically rotate the agitator.

[0005] The installation and setup of flexible bioprocessing bags in a bioreactor vessel, along with associated piping, filter heaters, impellers, and other components, can be a labor-intensive and time-consuming process. For example, existing bioreactor vessels pose accessibility issues, making it difficult to properly align and seat the impeller with the base of the bioreactor vessel. Multiple workers and ladders may also be required for installation, especially for the piping and filter heaters that are positioned at the top of the vessel. Furthermore, the lack of piping support for the various tubes that are connected to the flexible bag can result in a messy arrangement of tubing around the bioreactor vessel. In addition to the above, with existing systems, inflation and deflation of the consumable flexible bioprocessing bags is also a time-consuming process that can take anywhere between 10 minutes to nearly an hour.

[0006] In addition to the difficulty of installing the flexible bioprocessing bag and other components on the top of the bioreactor vessel, properly seating the base plate of the flexible bioprocessing bag's impeller on the bottom of the bioreactor vessel during installation of the flexible bag can also pose difficulties. Specifically, with existing systems, it has been difficult to ensure that the base plate of the flexible bag's impeller is properly seated within a recess in the bottom of the bioreactor vessel. display There is no feedback mechanism other than visual inspection to ensure that the base plate is properly installed. Even when visual inspection reveals that the base plate is properly installed, movement of the base plate is permitted prior to mating of the agitator with the magnetic drive mechanism below the vessel. Summary of the Invention [Problem to be solved by the invention]

[0007] In view of the above, there is a need for a piping and component management system for a bioprocessing system that is ergonomically efficient, facilitates installation and setup, and / or aids in the inflation and deflation of flexible bioprocessing bags. [Means for solving the problem]

[0008] In one embodiment, a bioreactor vessel includes a bottom and a peripheral sidewall, the bottom and peripheral sidewall defining an interior space for receiving a flexible bioprocess bag, a recess in the bottom for receiving a base plate of the flexible bioprocess bag, and a locking mechanism configured to hold the base plate in the recess.

[0009] In another embodiment, a bioprocessing apparatus includes a flexible bioprocessing bag and a base plate positioned at the bottom of the flexible bioprocessing bag and shaped to be received in a corresponding recess in the bottom of the bioreactor vessel, the base plate including a positioning mechanism adjacent a trailing edge of the base plate for cooperating with a corresponding positioning feature in the bottom of the bioreactor vessel adjacent the recess to position the base plate in the recess, and a locking mechanism extending downwardly from an underside of the base plate opposite the positioning mechanism for cooperating with a corresponding locking device on the bioreactor vessel to hold the base plate in the recess.

[0010] In yet another embodiment, a bioprocessing system includes a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent the recess, and a flexible bioprocessing bag positionable within the interior space, the flexible bioprocessing bag including a base plate at a bottom of the flexible bioprocessing bag. The base plate is configured to be received in the recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage with the base plate to retain the base plate in the recess. In one embodiment, the locking mechanism comprises a catch and the base plate includes a trap. The catch is movable between an engagement position in which the catch engages the trap when the base plate is positioned in the recess to retain the base plate in the recess, and a removal position in which the base plate may be withdrawn from the recess.

[0011] In yet another embodiment, a bioprocessing system includes a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent the recess; displayand a flexible bioprocess bag positionable within the interior space and comprising a base plate at its bottom. The base plate is configured to be received in a recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage with the base plate to retain the base plate in the recess. In one embodiment, the locking mechanism comprises a catch and the base plate includes a trap. The catch is movable between an engagement position where the catch engages with the trap when the base plate is positioned in the recess to retain the base plate in the recess, and a removal position where the base plate may be pulled out of the recess. display The mechanism operates when the base plate is properly positioned in the recess. display In one embodiment, the method is configured to: display The mechanism includes a plunger, a rocker arm, display The plunger is configured to be depressed when the base plate is properly positioned in the recess, and movement of the plunger is controlled by a rocker arm. display In a further embodiment, the rocker arm is configured to move from a position that prevents the locking feature from engaging the baseplate, corresponding to an improper position of the baseplate in the recess, to a position that allows the locking feature to freely engage the baseplate, corresponding to a proper position of the baseplate in the recess. display The mechanism operates when the base plate is properly positioned in the recess. display The sensor includes at least one sensor configured to:

[0012] In one embodiment, the bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel providing access to the interior space, and a piping and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system, the mounting frame being vertically movable into and out of the interior space.

[0013] In another embodiment, a method for installing a bioprocessing system component includes lowering a mounting frame into the vessel through a top opening of the vessel, opening an access door in a side wall of the vessel to access the mounting frame, loading at least one consumable component onto the mounting frame, closing the access door, and raising the mounting frame to a position adjacent to a top of the vessel.

[0014] In yet another embodiment, a bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel providing access to the interior space through the access door that is open, and a piping and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system. The mounting frame is movable between a mounting position in which the mounting frame is positioned within the interior space of the vessel at an elevation where the mounting frame is accessible through the access door, and an operating position in which the mounting frame is positioned generally above a top of the bioreactor vessel. The piping and component management apparatus includes a lifting mechanism for moving the mounting frame between the mounting position and the operating position.

[0015] The invention will be better understood from reading the following description of non-limiting embodiments, with reference to the accompanying drawings, in which: [Brief description of the drawings]

[0016] [Figure 1] FIG. 1 is a perspective view of a bioreactor system according to one embodiment of the present invention. [Diagram 2] FIG. 2 is another perspective view of the bioreactor system of FIG. 1 showing the access door in a closed position. [Diagram 3] FIG. 2 is another perspective view of the bioreactor system of FIG. 1 showing the access door in an open position. [Figure 4] FIG. 1 is a perspective view of a bioreactor system according to another embodiment of the present invention. [Diagram 5] FIG. 5 is a perspective view of the bioreactor system of FIG. 4 showing the access locations of the piping and component management devices. [Figure 6] FIG. 5 is a perspective view of the bioreactor system of FIG. 4 showing the loading position of the piping and component management device. [Figure 7] FIG. 5 is a perspective view of the bioreactor system of FIG. 4 showing the piping and consumable components installed in the component management device. [Figure 8] FIG. 5 is a perspective view of the bioreactor system of FIG. 4 showing the piping and component management equipment in ready position. [Figure 9] FIG. 5 is a perspective view of the bioreactor system of FIG. 4 showing the operational positions of the piping and component management apparatus. [Figure 10] FIG. 2 is a perspective view of a piping and component management apparatus according to another embodiment of the present invention. [Figure 11] FIG. 11 is an enlarged detailed view of the piping and component management device of FIG. [Figure 12] FIG. 11 is another enlarged detailed view of the piping and component management apparatus of FIG. [Figure 13] 11 is another enlarged detailed view of the piping and component management apparatus of FIG. 10. [Figure 14] FIG. 11 is a top plan view of a bioreactor vessel in which the piping and component management apparatus of FIG. 10 may be utilized. [Figure 15] FIG. 1 is a perspective view of a bioreactor system according to another embodiment of the present invention. [Figure 16] FIG. 6 is a perspective view of the bioreactor system of FIG. 5 showing the loading position of the piping and component management device. [Figure 17] FIG. 6 is a perspective view of the bioreactor system of FIG. 5 showing the piping and consumable components installed in the component management device. [Figure 18] FIG. 6 is a perspective view of the bioreactor system of FIG. 5 showing the piping and component management equipment in ready position. [Figure 19]FIG. 6 is a perspective view of the bioreactor system of FIG. 5 showing the operational positions of the piping and component management apparatus. [Figure 20] FIG. 1 is a perspective view of a base plate of a flexible bioprocess bag for use with a bioreactor system, according to one embodiment of the present invention. [Figure 21] FIG. 13 is a top perspective view of a locking system for a base plate of a bioreactor system according to another embodiment of the present invention. [Figure 22] FIG. 22 is a bottom perspective view of the locking system of FIG. 21. [Figure 23] FIG. 22 is a side elevation view of the locking system of FIG. 21. [Figure 24] FIG. 22 is a perspective view in cross section of a base plate locked in place in a bioreactor vessel using the locking system of FIG. 21. [Diagram 25] FIG. 22 is a side cross-sectional view of a base plate locked in place in a bioreactor vessel using the locking system of FIG. 21. [Figure 26] FIG. 13 is a top perspective view of a locking and indication system for a base plate of a bioreactor system according to another embodiment of the present invention. [Figure 27] FIG. 27 is a bottom perspective view of the locking and indicator system of FIG. 26. [Figure 28] FIG. 27 is a side view of the locking and indicating system of FIG. 26 when the base plate is improperly positioned. [Figure 29] FIG. 27 is a side view of the locking and indicating system of FIG. 26 when the base plate is properly positioned. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0017] Reference will now be made in detail to the exemplary embodiments of the invention, examples of which are illustrated in the accompanying drawings. Wherever possible, the same reference numbers are used throughout the drawings to refer to the same or like parts.

[0018] As used herein, the terms "flexible" or "foldable" refer to structures or materials that are pliable or can bend without cracking, and can also refer to materials that are compressible or expandable. An example of a flexible structure is a bag formed from a polyethylene film. The terms "rigid" and "semi-rigid" are used interchangeably herein to describe structures that are "non-collapsible", i.e., structures that do not fold, collapse, or deform under normal forces to substantially shorten their elongated dimensions. Depending on the context, "semi-rigid" can also refer to structures that are more flexible than "rigid" elements, such as bendable tubes or conduits, but still do not collapse in the longitudinal direction under normal conditions and forces.

[0019] The term "vessel" is used herein and may refer to flexible bags, flexible containers, semi-rigid containers, rigid containers, or flexible or semi-rigid tubing, as the case may be. The term "vessel" as used herein is intended to cover bioreactor vessels having walls or portions of walls, including, for example, cell culture / purification systems, mixing systems, media / buffer preparation systems, filtration / purification systems, such as chromatography and tangential flow filtration systems, and their associated flow paths, flexible or semi-rigid, single-use flexible bags, and other containers or conduits commonly used in biological or biochemical processes. As used herein, the term "bag" refers to a flexible or semi-rigid container or vessel used, for example, as a bioreactor or mixer for the contents therein. As used herein, "consumable" or "consumable component" refers to a device or component that is intended to be replaced periodically due to wear or use.

[0020] Embodiments of the present invention provide a bioprocessing system, and in particular a piping and component management system and devices for a bioreactor system. In one embodiment, a bioreactor vessel comprises a bottom and a peripheral sidewall, the bottom and peripheral sidewall defining an interior space for receiving a flexible bioprocess bag, a recess in the bottom for receiving a base plate of the flexible bioprocess bag, and a locking mechanism configured to hold the base plate in the recess.

[0021] Further embodiments of the present invention provide a bioprocessing system, and in particular a piping and component management system and device for a bioreactor system. In one embodiment, a bioreactor vessel includes a bottom and a peripheral sidewall, the bottom and peripheral sidewall defining an interior space for receiving a flexible bioprocess bag, a recess in the bottom for receiving a base plate of the flexible bioprocess bag, a locking mechanism configured to hold the base plate in the recess, and a locking mechanism configured to lock the base plate in the recess when the base plate is properly positioned in the recess. display It is configured to display The mechanism is also provided.

[0022] An embodiment of the present invention provides a bioprocessing system, and in particular a piping and component management system and device for a bioreactor system. In one embodiment, the bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a side wall of the vessel providing access to the interior space, and a piping and component management device mounted to the side wall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system. The mounting frame is vertically movable into and out of the interior space.

[0023] 1-3, a bioreactor system 10 according to one embodiment of the present invention is shown. The bioreactor system 10 includes a generally rigid bioreactor vessel or support structure 12 mounted on a base 14 having a plurality of legs 16. The vessel 12 may be formed, for example, from stainless steel, polymers, composites, glass, or other metals and may be cylindrical in shape, although other shapes may be utilized without departing from the broader aspects of the present invention. The vessel 12 may be of any shape or size so long as it is capable of supporting a single-use flexible bioreactor bag in its interior space 18. For example, according to one embodiment of the present invention, the vessel 12 is capable of receiving and supporting a 10 L to 2000 L flexible or collapsible bioprocess bag.

[0024] The vessel 12 may include one or more visual windows 20 that allow an operator to view the height of fluid in a flexible bag positioned in the interior space 18, and a window 22 positioned in a lower region of the vessel 12. The windows 22 allow access to the interior of the vessel 12 for insertion and positioning of various sensors and probes (not shown) within the flexible bag, as well as for connecting one or more fluid lines to the flexible bag for fluids, gases, etc., to be added to or withdrawn from the flexible bag. Sensors / probes and controls for monitoring and controlling critical process parameters include, for example, any one or more and combinations of temperature, pressure, pH, dissolved oxygen (DO), dissolved carbon dioxide (pCO2), mixing ratios, and gas flow rates.

[0025] As best shown in Figures 2 and 3, in one embodiment, the container 12 includes an access door 24 that is hinged or pivotally coupled to a sidewall of the container 12 and is movable between a closed position (Figure 2) and an open or access position (Figure 3) that allows access to the interior space 18. The door 24 may include a handle 26 to facilitate movement of the door between the open and closed positions. In one embodiment, the door 24 may be configured and positioned such that a lower edge of the door 24 forms an upper edge or boundary of the window 22 and / or a side edge of the door 24 forms an edge or boundary of the window 20 when the door 24 is in the closed position. By having the edge of the door 24 define one or more boundaries of the windows 22, 20, a continuous unobstructed access opening in the sidewall of the container is formed by the opening 20, the opening 22, and the open door 24 (i.e., the opening through which the door is received) when the door 24 is in the open position. Thus, the area of ​​the continuous access opening formed in the side wall of the container 12 when the door is in the open position is equal to the combined area of ​​the door 24, window 22, and window 20. This provides greater clearance and access to the interior space 18 than if the door and window were separated by a portion of the side wall of the container 12.

[0026] With further reference to FIG. 3, the interior sidewall of the vessel 12 may include one or more vertical baffles 28 that project into the interior space 18. The baffles 28 may be generally triangular in cross-section, although shapes and configurations known in the art may be utilized without departing from the broad aspects of the present invention. The baffles 28 are configured to contact and bias the flexible bag inwardly (when installed in the interior space 18) during bioprocessing operations for purposes known in the art. The bottom of the bioreactor vessel 12 includes a locating recess 30 for receiving an impeller base plate, as will be discussed in detail below.

[0027] As further shown in Figures 1-3, the bioreactor system 10 also includes a piping and component management apparatus 40. The apparatus 40 includes a support member 42 mounted to an exterior sidewall of the vessel 12 and extending generally vertically above an upper edge of the vessel 12. In one embodiment, the support member 42 may be mounted to the vessel using welds, bolts, screws, fasteners, or the like, although other means of attachment may be utilized without departing from the broader aspects of the invention. The apparatus 40 further includes a mast arm or boom 44 extending generally horizontally above the vessel 12 from a distal end of the support member 42, a guide sleeve 46 depending downwardly from a distal end of the boom 44, and a mounting plate 48 having a shaft 50 slidably received within the guide sleeve 46. In one embodiment, the apparatus 40 is configured such that the guide sleeve 46 and the shaft 50 of the mounting plate 48 are aligned with, i.e., coaxial with, a central axis or centerline of the vessel 12.

[0028] As best shown in FIG. 1 , the apparatus 40 also includes a lifting mechanism 52 operable to selectively raise and lower the mounting plate 48 within the vessel 12, such as in the direction indicated by arrow A. For example, in one embodiment, the lifting mechanism may include a cable 54 coupled to the shaft 50 of the mounting plate 48, extending through the guide sleeve 46, extending along (or within) the boom 44, and extending downwardly along (or within) the support member 42. A distal end of the cable is coupled to an actuator, which may be, for example, a manual crank, motor, or other drive member. The actuator is operable to selectively retract the cable 54 to raise the mounting plate 48, or deliver the cable 54 to lower the mounting plate 48 into the vessel 12.

[0029] 1-3, the mounting plate 48 may be generally T-shaped and include a substantially planar upper surface for mounting various components used in a bioprocessing operation, such as filters, filter heaters, and other consumables. The mounting plate 48 may also include an array of slots or openings configured to receive and hold various tubing configured for connection to a flexible bag received within the vessel 12. One or more hooks 56 (or other suitable coupling members) attached to the mounting plate 48 may be utilized to move and / or support the flexible bag.

[0030] In use, when placing a flexible bioprocessing bag prior to bioprocessing, an access door 24 in the sidewall of the vessel 12 can be opened to allow unobstructed access to the interior space 18 within the vessel 12. The lifting mechanism 52 can then be utilized to lower the mounting plate 48 into the vessel 12 and to a height that is easily accessible to an operator (e.g., approximately waist height). In this position, a flexible bioprocessing bag (not shown) can be placed inside the vessel 12 and attached to a hook 56 on the mounting plate 48. Also, various tubes that are connected to the bag (or that will be connected to the bag) can be organized and kept out of the way of an operator by routing them through slots / openings in the mounting plate 48. Additionally, various functional components such as filter heaters, filters, and other consumables can be attached to the mounting plate 48 by bolts or the like. In this regard, an actuator of the lifting mechanism 52 can be utilized to raise the mounting plate 48 to an operating position generally at or above the top of the vessel 12. The access door 24 can then be moved to a closed position and the bioprocessing operation can begin.

[0031] In one embodiment, the lifting mechanism 52 can interact with the control unit of the bioreactor system 10 such that upon selection of a defined action of "START" or "INFlate", the lifting mechanism 52 automatically raises the loading plate 48 to an operating position at the top / above the vessel 12. The position of the loading plate 48 can also act as a position stop limiting the degree to which the bag can be inflated. Similarly, upon completion of a bioprocessing run, upon selection of a defined action of "DEFLATE" or "END", the lifting mechanism 52 can be automatically controlled to lower the loading plate 48. In some embodiments, lowering the loading plate 48 could aid in the contraction of flexible bioprocessing bags, which has previously been a rather time-consuming process. For example, lowering the loading plate 48 onto the bag could exert a downward force on the bag, aiding in the contraction.

[0032] As such, the bioreactor system 10 of the present invention provides an ergonomic means for installing flexible bioprocessing bags, filters, filter heaters, and other consumables, and for organizing the various tubings that connect to the bags. In contrast to existing systems, installation of such components can be performed at waist height from the side of the bioreactor vessel 12, eliminating the need for multiple operators and ladders.

[0033] 4-9, there is shown a bioreactor system 100 according to another embodiment of the present invention. The bioreactor system 100 is generally similar in configuration to the bioreactor system 10 of FIGS. 1-3 and includes a generally rigid bioreactor vessel or support structure 112 mounted on a base 114 having a plurality of legs 116. The vessel 112, like the vessel 12, is capable of supporting a single-use flexible bioreactor bag in an interior space 118. The vessel 112 may also include one or more viewing windows 120 and a window 122 located in a lower region of the vessel 112, the purposes of which have been described above.

[0034] 1-3, the container 112 may also include an access door 124 that is hinged or pivotally coupled to a sidewall of the container 112 and is movable between a closed position and an open or access position that allows access to the interior space 118. The door 124 may also include a handle 126 that facilitates movement of the door between the open and closed positions. As previously discussed, the door 124 may be configured to define a portion of one or more boundaries of the windows 120, 122, as previously disclosed, to maximize the size of the opening in the sidewall of the container 112 when the door 124 is in the open position.

[0035] With further reference to FIG. 4, the interior sidewall of the vessel 112 may include one or more vertical baffles 128 that protrude into the interior space 118, as previously discussed.

[0036] As shown in FIG. 4, the bioreactor system 100, like the bioreactor system 10, includes a piping and component management device 140 that facilitates installation and organization of the piping and consumable components of the bioreactor system 100. The device 140 includes a linear actuator 142 mounted to an exterior sidewall of the vessel 112. The linear actuator 142 includes a shaft 144 that is selectively extendable and retractable in a vertical direction represented by arrow B, as will be discussed in detail below. In one embodiment, the linear actuator 142 can take any form capable of vertically moving the shaft 144, such as, for example, a lead screw, a pneumatic actuator, or a hydraulic actuator. In one embodiment, the actuator 142 can take the form of a cable and a hand crank or motor driven drive system, similar to those previously described in connection with FIGS. 1-3. Other linear motion devices known in the art may be utilized without departing from the broader aspects of the present invention.

[0037] The apparatus 140 further comprises a support frame 146 coupled to the shaft 144 and vertically movable therewith under the control of the linear actuator 142. As shown in FIG. 4, the support frame 146 is suspended above the top opening of the bioreactor vessel 112 via a generally L-shaped support structure 148. The support frame 146 comprises guide rails 150, the purpose of which will be explained later. The apparatus 140 also comprises a mounting frame 152 that is slidably coupled to the support frame 146, and more specifically, to the guide rails 150 of the support frame 146. The mounting frame 152 is horizontally movable away from and towards the central axis of the bioreactor vessel, as indicated by arrow C. In one embodiment, a locking pin 154 is utilized to selectively restrict (or permit) the horizontal movement of the mounting frame 152.

[0038] In use, when installing a flexible bioprocessing bag prior to bioprocessing, the piping and component management device 140 starts in an initial position with the linear actuator 142 extended so that the mounting frame 152 is positioned above the top opening of the bioreactor vessel 112, as shown in FIG. 4. The linear actuator 142 is then utilized to lower the mounting frame 152 into the interior space 118, as shown in FIG. 5. The L-shaped support arms 148 of the support frame 146 are configured such that when in the lowered position, the mounting frame 152 is positioned approximately at waist height off the floor and ergonomically accessible by an operator. As further shown in FIG. 5, the door 124 can then be opened to provide easy access to the interior space 118. Referring to FIG. 6, the locking pin 154 is then withdrawn and the mounting frame 152 can be moved horizontally along the guide rails 150 to an extended position in which the mounting frame 152 extends out of the interior space 118 of the vessel 112 through the open door. A locking pin 154, also shown in FIG. 6, can be utilized to lock the mounting frame 152 in its extended position.

[0039] In this position, the mounting frame 152 is easily accessible for loading of consumable components, including, for example, filters 160, filter heaters, etc., onto the mounting frame 152, as well as for routing and management of tubing, as shown in FIG. 7. As also shown in FIG. 7, a flexible bioprocessing bag 20 can be easily installed in the interior space 118 and supported by the mounting frame 152 through one or more hooks 162 coupled thereto (similar to that previously described in connection with FIGS. 1-3). Once all of the tubing is organized, the bag 20 is installed, and the various other components are securely mounted to the mounting frame 152, the locking pin 154 is withdrawn, the mounting frame 152 is pushed back into position within the interior space 118, and the locking pin is inserted to hold the mounting frame 152 in place. The door 124 is then closed, as shown in FIG. 8. Referring to FIG. 9, the linear actuator 142 is then utilized to move the mounting frame 152 and attached components to an operating position at a required height above the bioreactor vessel 112 (i.e., generally outside the interior space 118).

[0040] Similar to the previously disclosed embodiments, in one embodiment, the device 140 may be controlled by a control unit (not shown) of the bioreactor system 100 such that the mounting plate 152 may be automatically moved to an installation position (where the mounting plate 152 is extended through an open door), an operating position (above the bioreactor vessel 112), or a retracted position (e.g., moving downward continuously or intermittently as the bag 20 is retracted) depending on the selected mode of operation of the bioreactor system 100.

[0041] In addition to eliminating the need for ladders and multiple operators to install bioprocessing bags and other consumables, the bioreactor system 100 eliminates the need for operators to reach or bend into the interior spaces within the bioreactor vessel to install such components. Specifically, the tubing and component management device 140 can be moved vertically without a ladder into an easily accessible position through a door in the vessel sidewall, and a sliding mounting frame can be extended horizontally from the bioreactor vessel to provide greater ease of installation for such consumable components. Thus, the present invention provides easier and faster installation and also provides repeatability in the way bioprocessing bags are installed.

[0042] In one embodiment, rather than being mounted on the outside of the vessel as previously described, the piping and component management apparatus may be integral with one of the internal baffles (e.g., baffle 28 of vessel 12). FIGS. 10-13 show one such implementation of a piping and component management apparatus 200. The apparatus 200 includes a base plate 210 configured for mounting to the interior sidewall of the bioreactor vessel at a predetermined location on one of the baffles. For example, in one embodiment, as shown in FIG. 11, the base plate 210 may include a number of flanges 212 having slots configured to receive threaded studs protruding from the interior sidewall of the bioreactor vessel for mounting of the base plate to the vessel using nuts received on the threaded studs.

[0043] The apparatus 200 further comprises a linear motion rail 214 coupled to the base plate 210, such as via bolts or other fasteners, a linear motion block 216 slidably coupled to the rail 214 for linear vertical movement along the rail 214, a carrier plate 218 coupled to the linear motion block 216, and a mounting frame 220 coupled to the carrier plate 218. As shown in Figures 10 and 13, a baffle cover 222 surrounds the base plate 210, the rail 214, and the slide block 216 and defines a hollow interior space between the cover 222 and the base plate 210. The baffle cover 222 is shaped to provide substantially the same functionality and performance as a typical baffle, and may be, for example, generally triangular in cross section. As best shown in Figures 10 and 13, the baffle cover 222 comprises a slot 224 at its top through which the carrier plate 218 extends. The hollow interior and slots 224 allow for vertical movement of the slide block 216, carrier plate 218, and mounting frame 220 along the guide rails 214, as will be disclosed below.

[0044] 10-12, the apparatus 200 also includes a cable hoist 226 or other drive mechanism having a cable 228 coupled to the carriage plate 218. The cable hoist 226 may be manually or motor driven and is operable to either pay out or retract the cable 228 to selectively raise or lower the slide block 216, and thus the carriage plate 218 and mounting frame 220, along the rails 214 to adjust the vertical position of the mounting frame 220.

[0045] 10-13, the mounting frame 220 can be generally annular or semi-annular in shape, although other shapes and configurations are contemplated. The mounting frame 220 can include a number of filter retaining devices 230 for receiving and retaining filters 232, bag hooks, tubing retainers, and similar mounting mechanisms for mounting an array of consumable components to the mounting frame 220.

[0046] FIG. 14 is a top plan view showing an exemplary bioreactor vessel 250 in which the piping and component management apparatus 200 may be utilized. As shown, the vessel includes a number of internal baffles 252, 254, 256. In one embodiment, the baffle 252 opposite the access door 258 may be replaced by the piping and component management apparatus 200. The piping and component management apparatus 200 may be easily secured to the vessel sidewalls as previously disclosed, plus easily removable during shipping and in the field for maintenance. As shown in FIG. 10, the top of the mounting frame 220 is free of cables or structures, which makes it easier to install filters.

[0047] As with the previously disclosed embodiments, the apparatus 200 may be controlled by a bioreactor system control unit (not shown) such that the mounting frame can be automatically lowered to a mounting position prior to the start of a bioprocessing run, and automatically raised to an operating position at the start of such run.

[0048] 15-19 depict another bioreactor system 500 that is generally similar in configuration to the bioreactor system 100 of FIGS. 4-9, with like numerals indicating like parts. However, as shown, the piping and component management apparatus 140 includes a slightly different mounting frame for mounting the various consumable components, tubing, etc. Specifically, the apparatus 140 includes a connector box 510 and a floating frame 512 that is coupled to the connector box 510 at the top of the shaft 144 of the linear actuator 142. The floating frame 512 includes a lower frame member 514 that is semi-annular in shape and configured to support at least one consumable component, such as, for example, the flexible bioprocess bag 20. The floating frame 512 additionally includes an upper frame member 516 that is also semi-annular in shape and configured to support at least one consumable component, such as, for example, the filter 160 and a pinch valve assembly 518. The floating frame 512 and its frame members 514, 516 are mounted for vertical movement within the bioreactor vessel 112 into and out of the interior space 118 in the manner previously described.

[0049] Specifically, during use, when installing the flexible bioprocessing bag 20 prior to bioprocessing, the piping and component management device 140 starts in an initial position in which the linear actuator 142 is extended such that the floating frame 512 and its frame members 514, 516 are positioned above the top opening of the bioreactor vessel 112, as shown in Figure 15. The linear actuator 142 is then utilized to lower the floating frame 512 into the interior space 118, as shown in Figure 16. The door 124 may then be opened (although it is contemplated that it may be opened prior to lowering the floating frame 512).

[0050] In this position, the floating frame 512 is easily accessible through the open door for loading of consumable components, including, for example, the filter 160, filter heater, flexible bags 20, and pinch valve assemblies 518 for piping, to the upper and lower frame members 514, 516, as well as for routing and managing the piping, as shown in FIG. 17. As also shown in FIG. 17, the flexible bioprocessing bags 20 can be easily installed in the interior space 118 and supported by the lower frame member 514. Once all of the piping is organized, the bags 20 are installed, and the various other components are securely mounted to the floating frame 512, the door 124 is then closed, as shown in FIG. 18. Referring to FIG. 19, the linear actuator 142 is then utilized to move the floating frame 512 and attached components to an operating position at a required height above the bioreactor vessel 112 (i.e., generally outside the interior space 118).

[0051] Similar to the previously disclosed embodiments, in one embodiment, the apparatus 140 may be controlled by a control unit (not shown) of the bioreactor system 500 such that the floating frame 512 may be automatically moved to a set position (where the floating frame is received within the interior space 118 at approximately waist height of an operator), an operating position (above the bioreactor vessel 112), or a retracted position (e.g., moving downwardly continuously or intermittently as the bag 20 is retracted) depending on the selected mode of operation of the bioreactor vessel / bioprocess system 500.

[0052] The tubing and component management device embodiments described herein provide an ergonomic means for installing flexible bioprocessing bags, filters, filter heaters, and other consumables, and for organizing the various tubings that connect to the bags. In contrast to existing systems, installation of such components can be performed at waist height from the side of the bioreactor vessel 12, eliminating the need for multiple operators and ladders.

[0053] In addition to the difficulties of installing a flexible bioprocessing bag and other components on the top of a bioreactor vessel as indicated above, properly seating the impeller base plate of the flexible bioprocessing bag on the bottom of the bioreactor vessel during installation of the flexible bag can also pose difficulties. Thus, in addition to providing piping and component management for the top of the flexible bag (i.e., piping and components mounted above the flexible bag at the top of the bioreactor vessel), embodiments of the present invention also provide component management at the bottom of the flexible bag. Specifically, embodiments of the present invention provide positioning, locking, and retention mechanisms for locking the impeller base plate into a recess (e.g., impeller base plate recess 30 in FIG. 3) at the bottom of the bioreactor vessel, and for verifying that the base plate is properly positioned within the recess. display In order to display The mechanism is directed towards:

[0054] Referring to FIG. 20, there is shown a component management system 300 in the form of a locking mechanism for an impeller base plate 310 for a bioprocessing system. The system 300 comprises a base plate 310 that may be attached to the bottom of a flexible, single-use bioprocessing bag (not shown) for use in a stirred tank bioreactor system, such as those shown in FIGS. 1-19. The base plate 310 may be mounted into an opening in the bottom of the flexible bioprocessing bag, such as by welding, although other means of attachment may be utilized without departing from the broad aspects of the invention. As is known, the base plate is configured to be received in a corresponding base plate recess 312 in the bottom 314 of the bioreactor vessel. As is known, the base plate 310 acts as an interface between an impeller (not shown) mounted to the base plate 310 inside the flexible bag and a rotating magnetic drive head (not shown) outside the bag below the base plate 310. The base plate 310 may also include a harvest port 316 for connection of drain piping to drain the flexible bag, and a number of ports 318 for mounting spargers to the base plate 310 inside the flexible bag.

[0055] 20, in one embodiment, the base plate 310 may also include a positioning feature in the form of a slot 320 extending from the underside of the base plate 310. The slot 320 is configured to receive a corresponding tongue 322 that projects from the bottom 314 of the container into the rear portion of the recess 312. It is contemplated that in one embodiment, the components of the positioning feature may be reversed such that the base plate may have a tongue projection that is received in a corresponding slot or groove in the bottom 314 of the container.

[0056] 20, the base plate 310 also includes a catch feature on its underside opposite the slot 320. In one embodiment, the catch feature includes a pair of downwardly depending catches 324 configured to be received in corresponding locking / catch features in the bottom 314 of the container, i.e., catch openings 326 in the bottom 314 of the container. In one embodiment, the catches 324 are generally L-shaped and resilient such that when the base plate 310 is rotated downwardly about the tongues 322, the base plate 310 snaps into a seated position in the recess 312, and the catches 324 engage the bottom 314 of the container to lock the base plate 310 in place. The catches 324 are accessible underneath the container and can be pinched inwardly to release the base plate 310 from the recess 312. Although FIG. 20 shows two stops 324, three or more stops, or a single stop, may be utilized without departing from the broader aspects of the present invention.

[0057] In use, the flexible bag is inserted into the bioreactor vessel and the baseplate 310 is angled as shown in Figure 20 so that the tongues 322 are received into the slots 320 in the baseplate 310. The front of the baseplate 310 is then pressed downwards until the cleat 324 is received into the cleat opening 326, snapping it into place to hold the baseplate 310 in the recess. This ensures that the baseplate is restrained in all three axes.

[0058] 21-23, there is shown another component management system 400 in the form of a locking / retaining mechanism for an impeller base plate, according to another embodiment of the present invention. The system 400 includes a base plate 410 that is configured to be attached to the bottom of a flexible, single-use bioprocessing bag and received in a corresponding base plate recess in the bottom of a bioreactor vessel, as previously described. As is known, the base plate 410 may include a harvest port 416 for connection of drain tubing to drain the flexible bag, and a number of ports 418 for mounting spargers to the base plate 410 inside the flexible bag.

[0059] Similar to base plate 310 of Figure 20, base plate 410 includes a rear slot 420 extending from the underside of base plate 410 and configured to receive a corresponding tongue (not shown) that projects into a rear portion of a recess in the bottom of the bioreactor vessel. As best shown in Figures 22 and 23, base plate 410 also includes a front catch 430 that extends downwardly from the underside of base plate 410. In one embodiment, catch 430 is generally U- or L-shaped with a capture member 432 that lies in a plane generally parallel to the body of base plate 410.

[0060] As best shown in FIGS. 21-23, the system 400 also includes a retention mechanism 440 having a retention member 442 configured to engage the capture member 432 of the capture portion 430. As shown, the retention member 442 is attached to a distal end of an elongated shaft 444 opposite the handle 446. The shaft 444 extends through a housing 448 and is coupled to a spring, such as a coil spring 450, within the housing 448. The spring 450 biases the retention member 442 toward the capture member 432 on the underside of the base plate 410, away from the housing 448, as described below. The housing 448 is configured to mount to the exterior bottom of the bioreactor vessel adjacent the recess in the base plate. For example, in one embodiment, the housing 448 can include a mounting flange 452 for mounting the housing 448 to the bottom of the bioreactor vessel using bolts or screws.

[0061] 23, in one embodiment, the stop member 442 may be offset from the longitudinal axis defined by the shaft 444. As also shown, in one embodiment, the stop member 442 may have a slanted or angled contact surface 454. This surface functions to convert the downward force exerted by the capture member 432 on the stop member 442 into a horizontal force that pushes the shaft 44 rearward against the spring bias of the coil spring 450, as will be discussed later.

[0062] In use, the flexible bag is inserted into the bioreactor vessel and the base plate 410 is angled so that the tongue that projects into the recess in the bottom of the bioreactor vessel is received in the slot 420 of the base plate 410. The front of the base plate 410 is then pressed downward until the bottom of the capture member 432 contacts the angled surface 454 of the retention member 442 of the retention mechanism 440. By continuing to press the base plate 410 downward, the capture member 432 exerts a force on the angled surface 454 of the retention member 442, causing the retention member 442 and shaft 444 to move rearward against the spring bias of the coil spring 450. As the capture member 432 passes the lower edge of the angled surface 454, the spring bias of the coil spring 450 causes the shaft 444 and retention member 442 to translate forward in the direction of arrow A in FIG. In this position, the catch members 442 extend over the capture members 432, locking the baseplate 410 in place within the recess. This ensures that the baseplate is restrained in all three axes. In one embodiment, a handle 446 is provided to allow the baseplate 410 to be locked and unlocked. display To achieve this, visual features such as visually distinct lines or features may be provided.

[0063] 24 and 25 are cross-sectional views of the base plate 410 in a locked position in a recess 460 in the bottom of a bioreactor vessel 462. As shown, tongues 464 in the bottom of the vessel 462 are received in slots 420 and catches 442 engage catches 430 to retain the base plate 410 in the recess 460.

[0064] During unloading of the flexible bag, the operator need only pull on the handle 446 to move the shaft 444 and the latch member 442 against the spring bias to a position where the latch member 442 does not engage the catch 430. In this position, the base plate 410 is free to rotate out of the recess and can be removed.

[0065] 26-29, a locking / retaining mechanism for an impeller base plate and a locking mechanism for an impeller according to another embodiment of the present invention are shown. display Another component management system 500 in the form of a part is shown. The system 500 includes a base plate 510 that is configured to be attached to the bottom of a flexible, single-use bioprocessing bag and received in a corresponding base plate recess in the bottom of a bioreactor vessel, as previously described. As is known, the base plate 510 may include a harvest port 516 for connection of drain tubing to drain the flexible bag, and a number of ports 518 for mounting spargers to the base plate 510 inside the flexible bag.

[0066] Similar to base plate 310 of FIG. 20 and base plate 410 of FIG. 21, base plate 510 may include a rear slot (not shown) extending from an underside of base plate 510 and configured to receive a corresponding tongue (not shown) that projects into a rear portion of a recess in the bottom of the bioreactor vessel. As best shown in FIGs. 27-29, base plate 510 also includes a forward catch 530 that extends downwardly from the underside of base plate 510. In one embodiment, catch 530 is generally U- or L-shaped with a capture member 532 that lies in a plane generally parallel to the body of base plate 510.

[0067] As best shown in FIGS. 27-29, the system 500 also includes a retaining mechanism 540 having a retaining member 542 configured to engage the capture member 532 of the capture portion 530. As shown, the retaining member 542 is attached to a distal end of a shaft 544 opposite a handle 546. The shaft 544 extends through a housing 547 and is coupled to the handle 546. The housing 547 is configured to mount to the exterior bottom of the bioreactor vessel adjacent the recess in the base plate, as shown in FIG. 27. For example, in one embodiment, the housing 547 may include a mounting flange for attaching the housing 547 to the bottom of the bioreactor vessel using bolts or screws.

[0068] 27, in one embodiment, the retaining member 542 may be offset from the longitudinal axis defined by the shaft 544. As also shown, in one embodiment, the retaining member 542 may have a slanted or angled contact surface 545. To lock the base plate 510 to the bottom of the bioreactor vessel, the user may rotate the handle 546, which extends the shaft 554 and causes the retaining member 542 to engage the capture member 532, as best shown in FIGS.

[0069] The mechanical locking system 500 for the impeller base plate as previously described is display As best shown in Figures 26, 28, and 29, display Mechanism 550 includes a plunger 552 that extends into a recess through the bottom of the bioreactor vessel. Plunger 552 is attached to a rocker arm 554 that further extends into housing 547. Positioned at the end of rocker arm 554 is a display As best shown in Figures 26, 28, and 29, display The unit is mounted in a housing 547 as shown in FIG. display A panel or part of the housing 547 display A pointer (or other visual indicator) attached to a portion of the rocker arm 554 or to the end of the rocker arm 554 (e.g., a cone-shaped attachment) in conjunction with the panel. display (means). display The panel provides two visual indicators corresponding to proper and improper positioning of the base plate 510. display Provide regions (i.e., upper and lower portions). For example, the upper portion can be colored green while the lower portion can be colored red.

[0070] In use, prior to attachment of the base plate 510 to the bottom of the bioreactor vessel, the plunger 552 is in an extended position such that an upper portion 553 of the plunger 552 protrudes into a recess in the bioreactor vessel. This is achieved via a biasing mechanism 558 (e.g., a spring) that is positioned in a notch in the bioreactor vessel. In this configuration, when the base plate 510 is improperly positioned, the rocker arm 554 will tilt downward, as best shown in FIG. 28 . display Part 556 is display When the base plate 510 is properly positioned, the plunger 552 is pushed down into the recess, which causes the rocker arm 554 to pivot about the pivot arm 555, which in turn causes the base plate 510 to rotate. display Part 556 display The panel is raised in this way. display Part 556 is display Move up and down on the panel to check whether the base plate 510 is properly positioned. display to provide.

[0071] According to an alternative embodiment, display The mechanism 550 includes a sensor associated with the plunger or configured to replace the plunger. The sensor detects when the baseplate is properly positioned. display By way of example, the sensor may be configured to detect when the base plate is properly positioned. display The sensor may be a proximity sensor that emits a signal (e.g., light, electromagnetic radiation) that is configured to activate the base plate. By way of further example, the sensor may be a mechanical sensor (e.g., a mechanical switch) that is depressed / activated upon proper placement of the base plate. An output signal from the sensor indicates when the proper placement has occurred. display (e.g., visual, tactile, or auditory).

[0072] According to further alternative embodiments, the rocker arm is configured to move from a position that prevents the locking / retaining mechanism from engaging the baseplate, corresponding to improper and proper positions of the baseplate in the recess, respectively, to a position where the locking / retaining mechanism is free to engage the baseplate. By way of example, the rocker arm may include a portion that is configured to abut a portion of the locking / retaining mechanism of any of the previous embodiments such that when the baseplate is improperly positioned, the rocker arm prevents the locking mechanism from properly engaging the baseplate (e.g., prevents the handle 546 from pivoting fully into position). Only when the baseplate is properly positioned, thereby causing the rocker arm to move, is the locking mechanism permitted to engage the baseplate.

[0073] display While specific embodiments relating to the mechanism have been discussed above, further embodiments will be appreciated by those skilled in the art in light of the present disclosure. display The mechanism is within the scope of the present invention.

[0074] It is noted that the location of the plunger 552 (or sensor) in the recess in the bottom of the bioreactor vessel, according to the embodiment, is generally centrally positioned. According to a preferred embodiment, the plunger is positioned adjacent to a notch in the bottom of the bioreactor vessel near the center of the recess, as shown by FIG. 26. This is the preferred location, as it ensures that the plunger is only depressed when the base plate 510 is properly positioned. If the plunger were positioned peripherally, the plunger could be depressed even if the base plate 510 is improperly positioned. Advantageously, display The mechanism 550 provides a clear indication of whether the base plate is properly positioned within the cavity. display to provide. display The mechanism further addresses potential problems associated with the difficulty in knowing whether the base plate is properly positioned even when the front or rear of the base plate is retained in the recess.

[0075] The mechanical locking system for the impeller base plate described herein provides a means for securely locking the impeller base plate into the bottom recess of the bioreactor vessel during flexible bag installation. The previously described mechanism also provides a tactile indication that the base plate is seated and in a locked position within the recess. display , Visual display or other display As also described above, while being securely locked to the vessel, the base plate can still be easily removed upon completion of the bioprocessing run.

[0076] It is contemplated that the base plate locking system described herein in connection with Figures 20-29 may be incorporated into any stirred tank bioreactor vessel known in the art. It is further contemplated that the base plate locking system of the present invention may be incorporated into any bioreactor vessel shown in Figures 2-19 that includes a plumbing and component management system as described herein. Specifically, in one embodiment, the bioreactor vessel may include one or both of a base plate locking mechanism for securing the base plate to the bottom of the bioreactor vessel and a plumbing and component management system for positioning and securing consumable components above the flexible bag. The combination of the base plate locking mechanism and the plumbing and component management system provides a bioreactor vessel that facilitates installation of consumable components and provides an ease of use not previously seen in the art.

[0077] In one embodiment, a bioreactor vessel is provided having a bottom and a peripheral sidewall, the bottom and peripheral sidewall defining an interior space for receiving a flexible bioprocess bag, a recess in the bottom for receiving a base plate of the flexible bioprocess bag, and a locking mechanism configured to hold the base plate in the recess and lock the base plate when the base plate is properly positioned in the recess. display It is configured to displayand a mechanism for removing the base plate from the bioreactor vessel. In one embodiment, the locking mechanism comprises a catch that is movable between an engagement position in which the catch engages the base plate when the base plate is positioned in a recess in the bottom of the bioreactor vessel to retain the base plate in the recess, and a removal position in which the base plate may be pulled out of the recess. In one embodiment, the catch is spring biased towards the engagement position. In one embodiment, the locking mechanism includes a handle operable to move the catch from the engagement position to the removal position. In one embodiment, the catch comprises an angled upper surface configured to convert a downward force from the base plate during installation of the base plate in the recess into a lateral force for moving the catch against the spring bias to the removal position. In one embodiment, display The mechanism includes a plunger, a rocker arm, display In one embodiment, the plunger initially extends into the recess. In one embodiment, when the base plate is properly positioned in the recess, display The part moves in the opposite direction to the movement of the plunger through the rocker arm, which is displayIn one embodiment, the bioreactor vessel includes a tongue extending into the recess opposite the locking mechanism and configured to engage a slot in a rear region of the base plate. In one embodiment, the locking mechanism includes at least one opening adjacent the recess and configured to receive at least one corresponding catch in the base plate. In one embodiment, the at least one opening is a pair of openings. In another embodiment, a bioprocessing apparatus is provided. The apparatus includes a flexible bioprocessing bag and a base plate positioned at a bottom of the flexible bioprocessing bag and shaped to be received in a corresponding recess in the bottom of the bioreactor vessel. The base plate includes a positioning mechanism adjacent a rear edge of the base plate for cooperating with a corresponding positioning feature in the bottom of the bioreactor vessel adjacent the recess to position the base plate in the recess, and a locking mechanism extending downwardly from an underside of the base plate opposite the positioning mechanism for cooperating with a corresponding locking device of the bioreactor vessel to hold the base plate in the recess. In one embodiment, the positioning feature is one of a slot and a tongue, and the corresponding positioning feature is the other of the slot and the tongue. In one embodiment, the locking feature is a catch that lies in a plane generally parallel to and spaced from the body of the base plate, and the locking device includes a catch configured to engage the catch. In one embodiment, the locking feature is at least one catch member, and the locking device includes a recess configured to receive the at least one catch member. In one embodiment, the at least one catch member is generally L-shaped. In one embodiment, the at least one catch member is resilient.

[0078] In yet another embodiment, a bioprocessing system is provided, comprising: a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent the recess; displayand a flexible bioprocess bag positionable within the interior space and comprising a base plate at its bottom. The base plate is configured to be received in a recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage with the base plate to retain the base plate in the recess. In one embodiment, the locking mechanism comprises a catch and the base plate comprises a trap. The catch is movable between an engagement position where the catch engages with the trap when the base plate is positioned in the recess to retain the base plate in the recess, and a removal position where the base plate may be pulled out of the recess. In one embodiment, the catch is spring biased towards the engagement position. In one embodiment, the locking mechanism comprises a handle operable to move the catch from the engagement position to the removal position. In one embodiment, the catch comprises an angled upper surface configured to convert a downward force from the trap of the base plate during installation of the base plate in the recess into a lateral force to move the catch against the spring bias to the removal position. In one embodiment, the system may also include a tongue extending into the recess opposite the locking feature and configured to engage a slot in the rear region of the base plate. In one embodiment, the locking feature includes at least one aperture adjacent the recess and configured to receive at least one catch depending downwardly from the base plate opposite the slot. In one embodiment, display The mechanism includes a plunger, a rocker arm, display In one embodiment, the plunger initially extends into the recess. In one embodiment, when the base plate is properly positioned in the recess, display The part moves in the opposite direction to the movement of the plunger through the rocker arm, which is display to provide.

[0079] In one embodiment, a bioprocessing system is provided. The bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel providing access to the interior space, and a piping and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system. The mounting frame is vertically movable into and out of the interior space. In one embodiment, the mounting frame is movable between a set position in which the mounting frame is positioned within the interior space of the vessel at an elevation where the mounting frame is accessible through the access door, and an operating position in which the mounting frame is generally positioned above an upper portion of the bioreactor vessel. In one embodiment, the piping and component management apparatus includes an elevation mechanism for vertically moving the mounting frame along a centerline of the vessel. In one embodiment, the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a storage position in which the mounting frame is positioned within the interior space and an access position in which the mounting frame extends through an open access door when the access door is in an open position. In one embodiment, the piping and component management apparatus comprises a locking device for selectively locking the mounting frame in the stowed position and the access position. In one embodiment, the lifting mechanism is a linear actuator. In one embodiment, the piping and component management apparatus comprises a support member mounted to a side wall of the vessel, a boom extending from the support member generally above the vessel, and a sleeve extending from the boom downwardly along a centerline of the vessel, the mounting frame comprising a shaft received within the sleeve. In one embodiment, the lifting mechanism comprises a cable extending from the mounting frame through the sleeve and along the boom, the cable being selectively extendable and retractable to selectively lower and raise the mounting frame. In one embodiment, the lifting mechanism is integral with an interior baffle of the vessel. In one embodiment, the piping and component management apparatus comprises a guide rail mounted to an interior side wall of the vessel and a carrier plate slidably coupled to the guide rail, the mounting frame coupled to the carrier plate for vertical movement along the guide rail.In one embodiment, the piping and component management apparatus comprises a baffle cover defining an internal baffle, the baffle cover comprising a slot through which the carrier plate extends. In one embodiment, the vessel comprises a window in a sidewall of the vessel. The access door is movable between a closed position and an open position. In the closed position, an edge of the access door defines at least a portion of a boundary of the window. In one embodiment, the mounting frame comprises at least one slot, opening, or bracket for receiving the at least one consumable component. In one embodiment, the at least one consumable component is a tube, a filter, or a filter heater.

[0080] In another embodiment, a method for installing a component of a bioprocessing system is provided. The method includes lowering a mounting frame into the vessel through a top opening of the vessel, opening an access door in a sidewall of the vessel to access the mounting frame, loading at least one consumable component onto the mounting frame, closing the access door, and raising the mounting frame to a position adjacent to a top of the vessel. In one embodiment, the method also includes moving the mounting frame in a direction generally perpendicular to a centerline of the vessel to extend the mounting frame through the open access door. In one embodiment, the steps of lowering the mounting frame and raising the mounting frame are performed automatically by a control unit of the bioprocessing system. In one embodiment, the method also includes actuating a lifting assembly to lower or raise the mounting frame.

[0081] In yet another embodiment, a bioprocessing system is provided. The bioprocessing system includes a vessel defining an interior space for receiving flexible bioprocessing bags, the vessel having an access door in a sidewall of the vessel providing access to the interior space through the open access door, and a piping and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system. The mounting frame is movable between a set position in which the mounting frame is positioned within the interior space of the vessel at an elevation where the mounting frame is accessible through the access door, and an operating position in which the mounting frame is generally positioned above an upper portion of the bioreactor vessel. The piping and component management apparatus includes a lifting mechanism for moving the mounting frame between the set position and the operating position. In one embodiment, the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a stowed position in which the mounting frame is positioned within the interior space and an access position in which the mounting frame extends through the open access door when the access door is in the open position. In one embodiment, the piping and component management apparatus is mounted inside the sidewall of the vessel. In one embodiment, the lifting mechanism includes a linear actuator. In one embodiment, the container includes a window in a sidewall of the container, and the access door is movable between a closed position and an open position, and when in the closed position, an edge of the access door defines at least a portion of the boundary of the window.

[0082] As used herein, elements or steps referred to in the singular and elements or steps preceded by the words "a" or "an" should be understood as not excluding a plurality of said elements or steps, unless expressly stated otherwise. Furthermore, references to "one embodiment" of the invention are not intended to be interpreted as excluding the existence of additional embodiments that also incorporate the claimed features. Furthermore, unless expressly stated to the contrary, embodiments "comprising," "including," or "having" an element or elements having a particular property may include additional such elements that do not have that property.

[0083] This written description uses examples to disclose some embodiments of the invention, including the best mode, and to enable one of ordinary skill in the art to practice embodiments of the invention, including making any device or system, using any device or system, and performing any incorporated methods. The patentable scope of the invention is defined by the claims, and may include other examples that occur to those of ordinary skill in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that do not differ from the language of the claims, or if they include equivalent structural elements with insubstantial differences from the language of the claims.

[0084] [Section 1] The bottom and a peripheral sidewall, the base and the peripheral sidewall defining an interior space for receiving a flexible bioprocess bag; a recess in the bottom for receiving a base plate of the flexible bioprocess bag; a locking mechanism configured to hold the base plate in the recess; A bioreactor vessel comprising: [Section 2] The locking mechanism includes a locking portion, The bioreactor vessel described in paragraph 1, wherein the anti-skid portion is movable between an engagement position in which the anti-skid portion engages with the base plate when the base plate is positioned in the recess in the bottom of the bioreactor vessel to retain the base plate in the recess, and a removal position in which the base plate can be pulled out of the recess. [Section 3] 3. The bioreactor vessel of claim 2, wherein the stopper is spring biased toward the engaged position. [Section 4] 4. The bioreactor vessel of claim 2 or 3, wherein the locking mechanism includes a handle operable to move the catch from the engagement position to the removal position. [Section 5] 5. The bioreactor vessel of claim 3 or 4, wherein the stop comprises an angled upper surface configured to convert a downward force from the base plate during placement of the base plate in the recess into a lateral force to move the stop against the spring bias to the removal position. [Section 6] a tongue extending into the recess opposite the locking mechanism, the tongue configured to engage a slot in a rear region of the base plate; 6. The bioreactor vessel of any one of claims 1 to 5, further comprising: [Section 7] 7. The bioreactor vessel of any one of claims 1 to 6, wherein the locking mechanism includes at least one opening adjacent the recess and configured to receive at least one corresponding stop of the base plate. [Section 8] 8. The bioreactor vessel of clause 7, wherein the at least one opening is a pair of openings. [Section 9] 9. The bioreactor vessel of any one of the preceding paragraphs, further comprising an indication mechanism configured to indicate whether the base plate of the flexible bioprocess bag is properly positioned within the recess. [Section 10] The display mechanism includes: a plunger positioned within the base, an upper portion of the plunger configured to protrude into the recess; a rocker arm having a first end and a second end, the first end coupled to the plunger; 10. The bioreactor vessel of paragraph 9, comprising: [Section 11] the second end of the rocker arm includes an indicator configured to move in response to movement of the plunger; 11. The bioreactor vessel of paragraph 10, wherein movement of the indicia provides an indication that the base plate is properly or improperly positioned within the recess. [Section 12] A flexible bioprocessing bag; a base plate positioned at the bottom of the flexible bioprocess bag and shaped to be received in a corresponding recess in the bottom of a bioreactor vessel, a positioning mechanism adjacent a rear edge of the base plate for cooperating with a corresponding positioning feature on the bottom of the bioreactor vessel adjacent the recess to position the base plate in the recess; a locking mechanism extending downwardly from an underside of the base plate opposite the positioning mechanism for cooperating with a corresponding locking device of the bioreactor vessel to hold the base plate in the recess; and Including base plate and A bioprocessing apparatus comprising: [Section 13] the positioning feature being one of a slot and a tongue; 13. The bioprocess apparatus of clause 12, wherein the corresponding positioning feature is the other of a slot and a tongue. [Section 14] the locking mechanism is a catch that lies in a plane generally parallel to and spaced from the body of the baseplate; 14. The bioprocess apparatus of clause 12 or clause 13, wherein the locking device comprises a catch configured to engage with the catch. [Section 15] The locking mechanism is at least one locking member, 14. The bioprocess apparatus of clause 12 or clause 13, wherein the locking device comprises a recess configured to receive the at least one retention member. [Section 16] 16. The bioprocess apparatus of clause 15, wherein the at least one retention member is generally L-shaped. [Section 17] 17. The bioprocess apparatus of clause 15 or clause 16, wherein the at least one retention member is resilient. [Section 18] 18. The bioprocessing apparatus of any one of clauses 12 to 17, further comprising an indication mechanism configured to indicate whether the base plate of the flexible bioprocessing bag is properly positioned within the recess of the bioreactor vessel. [Section 19] The display mechanism includes: a plunger configured to be positioned within the base, an upper portion of the plunger configured to protrude into the recess; a rocker arm having a first end and a second end, the first end coupled to the plunger; 20. The bioprocessing apparatus of claim 18, comprising: [Section 20] the second end of the rocker arm includes an indicator configured to move in response to movement of the plunger; 20. The bioprocess apparatus of clause 19, wherein movement of the indicia provides an indication that the base plate is properly or improperly positioned within the recess. [Section 21] a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent to the recess; a flexible bioprocess bag positionable within the interior space, the flexible bioprocess bag including a base plate at a bottom of the flexible bioprocess bag; Equipped with the base plate is configured to be received in the recess in the bottom of the bioreactor vessel; The locking mechanism is configured to engage the base plate to retain the base plate in the recess. [Section 22] The locking mechanism includes a locking portion, The base plate includes a catch portion; 22. The bioprocessing system of claim 21, wherein the anti-skid portion is movable between an engagement position in which the anti-skid portion engages with the capture portion when the base plate is positioned in the recess to retain the base plate in the recess, and a removal position in which the base plate can be pulled out of the recess. [Section 23] 23. The bioprocessing system of claim 22, wherein the stop is spring biased towards the engaged position. [Section 24] 24. The bioprocessing system of claim 22 or 23, wherein the locking mechanism comprises a handle operable to move the catch from the engagement position to the removal position. [Section 25] 25. The bioprocessing system of any one of paragraphs 22 to 24, wherein the stop comprises an angled upper surface configured to convert a downward force from the catch of the base plate during installation of the base plate in the recess into a lateral force to move the stop against the spring bias to the removal position. [Section 26] a tongue extending into the recess opposite the locking mechanism, the tongue configured to engage a slot in a rear region of the base plate; 26. The bioprocessing system of any one of clauses 21 to 25, further comprising: [Section 27] 27. The bioprocessing system of claim 26, wherein the locking mechanism includes at least one aperture adjacent the recess and configured to receive at least one catch depending downwardly from the base plate opposite the slot. [Section 28] 28. The bioprocessing system of any one of clauses 22 to 27, further comprising an indication mechanism configured to indicate whether the base plate of the flexible bioprocessing bag is properly positioned within the recess of the bioreactor vessel. [Section 29] The display mechanism includes: a plunger configured to be positioned within the base, an upper portion of the plunger configured to protrude into the recess; a rocker arm having a first end and a second end, the first end coupled to the plunger; 30. The bioprocessing system of claim 28, comprising: [Section 30] the second end of the rocker arm includes an indicator configured to move in response to movement of the plunger; 30. The bioprocessing system of claim 29, wherein movement of the indicia provides an indication that the base plate is properly or improperly positioned within the recess. [Section 31] 1. A bioprocessing system comprising: a container defining an interior space for receiving a flexible bioprocessing bag, the container having an access door in a sidewall of the container providing access to the interior space; a piping and component management device mounted to the side wall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system; Equipped with The mounting frame is vertically movable into and out of the interior space. [Section 32] 32. The bioprocessing system of claim 31, wherein the mounting frame is movable between an installation position in which the mounting frame is positioned within the interior space of the vessel at an elevation where the mounting frame is accessible through the access door, and an operating position in which the mounting frame is positioned generally above a top of the vessel. [Section 33] 33. The bioprocessing system of claim 31 or 32, wherein the piping and component management device includes a lifting mechanism for vertically moving the mounting frame along a centerline of the vessel. [Section 34] 34. The bioprocessing system of any one of clauses 31 to 33, wherein the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a stowed position in which the mounting frame is positioned within the interior space and an access position in which the mounting frame extends through an open access door when the access door is in an open position. [Section 35] 35. The bioprocessing system of any one of clauses 31 to 34, wherein the piping and component management apparatus includes a locking device for selectively locking the mounting frame in the stowed position and the access position. [Section 36] 36. The bioprocessing system of any one of clauses 33 to 35, wherein the lifting mechanism is a linear actuator. [Section 37] the piping and component management apparatus includes a support member mounted to a sidewall of the vessel, a boom extending from the support member generally above the vessel, and a sleeve extending downwardly from the boom along a centerline of the vessel; 37. The bioprocessing system of any one of clauses 33 to 36, wherein the mounting frame includes a shaft that is received within the sleeve. [Section 38] the lifting mechanism includes a cable extending from the mounting frame through the sleeve and along the boom; 38. The bioprocessing system of any one of clauses 33 to 37, wherein the cables are selectively extendable and retractable to selectively lower and raise the mounting frame. [Section 39] 40. The bioprocessing system of any one of clauses 33 to 38, wherein the lifting mechanism is integral with an internal baffle of the vessel. [Section 40] the piping and component management device includes a guide rail mounted to an interior sidewall of the vessel and a carrier plate slidably coupled to the guide rail; 40. The bioprocessing system of any one of clauses 33 to 39, wherein the mounting frame is coupled to the carrier plate for vertical movement along the guide rails. [Section 41] the piping and component management apparatus includes a baffle cover that defines the internal baffle; 41. The bioprocessing system of claim 39 or 40, wherein the baffle cover includes a slot through which the carrier plate extends. [Section 42] the container includes a window in a sidewall of the container; the access door is movable between a closed position and an open position; 42. The bioprocessing system of any one of clauses 31 to 41, wherein when in the closed position, an edge of the access door defines at least a portion of a boundary of the window. [Section 43] 43. The bioprocessing system of any one of clauses 31 to 42, wherein the mounting frame includes at least one slot, opening, or bracket for receiving a component of the at least one consumable. [Section 44] 44. The bioprocessing system of clause 43, wherein the at least one consumable component is a tube, a filter, or a filter heater. [Section 45] 45. The bioprocess system according to any one of clauses 31 to 44, wherein the vessel is a bioreactor vessel according to any one of clauses 1 to 8. [Section 46] 1. A method for installing components of a bioprocessing system, comprising: lowering the mounting frame into the container through a top opening of the container; opening an access door in a side wall of the container to access the mounting frame; mounting at least one consumable component onto the mounting frame; closing the access door; raising the mounting frame to a position adjacent an upper portion of the container; A method comprising: [Section 47] moving the mounting frame in a direction generally perpendicular to a centerline of the container to extend the mounting frame through the open access door. 47. The method of claim 46, further comprising: [Section 48] 48. The method of claim 46 or 47, wherein the steps of lowering the mounting frame and raising the mounting frame are performed automatically by a control unit of the bioprocessing system. [Section 49] 49. The method of any one of clauses 46 to 48, further comprising actuating a lifting assembly to lower or raise the mounting frame. [Section 50] 1. A bioprocessing system comprising: a container defining an interior space for receiving a flexible bioprocessing bag, the container having an access door in a sidewall of the container providing access to the interior space through the access door being open; a piping and component management device mounted to the side wall of the vessel and having a mounting frame for mounting at least one consumable component of the bioprocessing system; Equipped with the mounting frame is movable between an installed position in which the mounting frame is positioned within the interior space of the container at an elevation where the mounting frame is accessible through the access door, and an operational position in which the mounting frame is positioned generally above a top of the container; The piping and component management apparatus of the bioprocessing system includes a lifting mechanism for moving the mounting frame between the installation position and the operating position. [Section 51] 51. The bioprocessing system of claim 50, wherein the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a stowed position in which the mounting frame is positioned within the interior space and an access position in which the mounting frame extends through an open access door when the access door is in an open position. [Section 52] 52. The bioprocessing system of claim 50 or 51, wherein the piping and component management device is mounted inside the side wall of the vessel. [Section 53] 53. The bioprocessing system of any one of clauses 50 to 52, wherein the lifting mechanism comprises a linear actuator. [Section 54] the container includes a window in a sidewall of the container; the access door is movable between a closed position and an open position; 54. The bioprocessing system of any one of clauses 50 to 53, wherein when in the closed position, an edge of the access door defines at least a portion of the boundary of the window. [Section 55] 55. The bioprocessing system according to any one of clauses 50 to 54, wherein the vessel is a bioreactor vessel according to any one of clauses 1 to 8. [Explanation of symbols]

[0085] 10 Bioreactor System 12 Bioreactor vessel, supporting structure 14 Basics 16 Legs 18 Interior Space 20 Visual window, opening 20 Flexible Bioprocess Bags 22 Windows and openings 24 Access Door 26 Handle 28 Baffle 30. Depression 40 Piping and component management devices 42 Support member 44 Mast arm, boom 46 Guide sleeve 48 Mounting Plate 50 Shaft 52 Lifting mechanism 54 Cable 56 Hook 100 Bioreactor System 112 Bioreactor vessel, supporting structure 114 Basics 116 Legs 118 Interior Space 120 Visual Window 122 Window 124 Access Door 126 Handle 128 Baffle 140 Piping and component management devices 142 Linear Actuator 144 Shaft 146 Support Frame 148 Support structure, support arm 150 Guide rail 152 Mounting frame, mounting plate 154 Locking pin 160 Filters 200 Piping and component management devices 210 Foundation plate 212 Flange 214 Linear motion rails, guide rails 216 Linear Motion Block 218 Transport Plate 220 Mounting Frame 222 Baffle cover 224 Slots 226 Cable Winder 228 Cable 230 Filter Retaining Device 232 Filter 250 Bioreactor Vessel 252, 254, 256 Internal Baffles 258 Access Door 300 Component Management System 310 Foundation plate 312 Depression 314 Bottom 316 Collection Port 318 Port 320 Slots 322 Tongue 324 Stopper 326 Anti-fall opening 400 Component Management System 410 Foundation plate 416 Collection Port 418 Port 420 Rear Slot 430 Capture Department 432 Capturing member 440 Anti-detachment mechanism 442 Anti-slip member 444 Shaft 446 Handle 448 Case 450 coil spring 452 Mounting flange 454 Contact Surface, Angled Surface 460 Depression 462 Bioreactor vessel 464 Tongue 500 Bioreactor System 510 Connector Box 512 Floating Frame 514 Lower frame member 516 Upper frame member 518 Pinch Valve Assembly 500 Component Management System 510 Foundation plate 516 Collection Port 518 port 530 Capture Department 532 Capturing member 540 Anti-fall mechanism 542 Anti-fall member 544 Shaft 545 Contact surface 546 Handle 547 Case 550 display mechanism 552 Plunger 553 Top 554 Rocker arm 555 Pivot Arm 556 display Department 558 Biasing mechanism

Claims

1. A flexible bioprocessing bag; a base plate positioned at the bottom of the flexible bioprocess bag and shaped to be received in a corresponding recess in the bottom of a bioreactor vessel, a positioning mechanism adjacent a rear edge of the base plate for cooperating with a corresponding positioning feature on the bottom of the bioreactor vessel adjacent the recess to position the base plate in the recess; a locking mechanism extending downwardly from an underside of the base plate opposite the positioning mechanism for cooperating with a corresponding locking device of the bioreactor vessel to hold the base plate in the recess; and Including base plate and A bioprocessing apparatus comprising:

2. the positioning feature being one of a slot and a tongue; 10. The bioprocess apparatus of claim 1, wherein the corresponding positioning feature is the other of a slot and a tongue.

3. the locking mechanism is a catch that lies in a plane generally parallel to and spaced from the body of the baseplate; 3. The bioprocess apparatus of claim 1, wherein the locking device comprises a catch configured to engage the catch.

4. The locking mechanism is at least one locking member, 3. The bioprocess apparatus of claim 1, wherein the locking device comprises a recess configured to receive the at least one retention member.

5. 5. The bioprocess apparatus of claim 4, wherein the at least one retention member is generally L-shaped.

6. 6. The bioprocess apparatus of claim 4 or 5, wherein the at least one retaining member is resilient.

7. 7. The bioprocess apparatus of any one of claims 1 to 6, further comprising an indication mechanism configured to indicate whether the base plate of the flexible bioprocess bag is properly positioned within the recess of the bioreactor vessel.

8. The display mechanism includes: a plunger configured to be positioned within the base, an upper portion of the plunger configured to protrude into the recess; a rocker arm having a first end and a second end, the first end coupled to the plunger; 8. The bioprocessing apparatus of claim 7, comprising:

9. the second end of the rocker arm includes an indicator configured to move in response to movement of the plunger; 10. The bioprocess apparatus of claim 8, wherein movement of the indicia provides an indication that the base plate is properly or improperly positioned within the recess.

10. a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent to the recess; a flexible bioprocess bag positionable within the interior space, the flexible bioprocess bag including a base plate at a bottom of the flexible bioprocess bag; Equipped with the base plate is configured to be received in the recess in the bottom of the bioreactor vessel; The locking mechanism is configured to engage the base plate to retain the base plate in the recess.

11. The locking mechanism includes a locking portion, The base plate includes a catch portion; 11. The bioprocessing system of claim 10, wherein the anti-skid portion is movable between an engagement position in which the anti-skid portion engages with the capture portion when the base plate is positioned in the recess to retain the base plate in the recess, and a removal position in which the base plate can be pulled out of the recess.

12. 12. The bioprocessing system of claim 11, wherein the stop is spring biased toward the engaged position.

13. 13. The bioprocessing system of claim 11 or 12, wherein the locking mechanism comprises a handle operable to move the catch from the engagement position to the removal position.

14. 14. The bioprocessing system of claim 11, wherein the stop comprises an angled upper surface configured to convert a downward force from the catch of the base plate during installation of the base plate in the recess into a lateral force to move the stop against the spring bias to the removal position.

15. a tongue extending into the recess opposite the locking mechanism, the tongue configured to engage a slot in a rear region of the base plate; 15. The bioprocessing system of claim 11, further comprising:

16. 16. The bioprocessing system of claim 15, wherein the locking mechanism includes at least one aperture adjacent the recess and configured to receive at least one detent depending downwardly from the base plate opposite the slot.

17. 17. The bioprocessing system of any one of claims 11 to 16, further comprising an indication mechanism configured to indicate whether the base plate of the flexible bioprocessing bag is properly positioned within the recess of the bioreactor vessel.

18. The display mechanism includes: a plunger configured to be positioned within the base, an upper portion of the plunger configured to protrude into the recess; a rocker arm having a first end and a second end, the first end coupled to the plunger; 20. The bioprocessing system of claim 17, comprising:

19. the second end of the rocker arm includes an indicator configured to move in response to movement of the plunger; 20. The bioprocessing system of claim 18, wherein the movement of the indicia provides an indication that the base plate is properly or improperly positioned within the recess.

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