Method for producing yeast cell wall particles

Abstract

To solve the problem in which, when β-glucan derived from yeast is used as a filler, conventional methods result in problems such as water solubility making it difficult to use, non-uniform particle size, or the presence of proteins limiting the allowable addition amount to very low levels.SOLUTION: The present invention relates to a method for producing yeast cell wall microparticles. Specifically, yeast cells or yeast cells after yeast extract extraction are subjected to NaOH treatment, and the resulting dewatered cake obtained through solid-liquid separation is treated with NaClO. The dewatered cake obtained through another round of solid-liquid separation is then concentrated and spray-dried to obtain yeast cell wall particles. This makes it possible to provide yeast cell wall microparticles as a uniform filler with good skin feel containing β-glucan as the main component while retaining the original yeast shape. Furthermore, since contained proteins that may act as allergens are extracted to the lower limit, the particles are suitable for use in cosmetics.SELECTED DRAWING: None

Description

Technical Field

[0001] The present disclosure relates to a method for producing yeast cell wall particles mainly composed of β-glucan with the cell wall structure derived from yeast maintained in shape for cosmetics.

Background Art

[0002] Yeast is used in a wide range of fields such as biofuels, chemicals, and pharmaceuticals, and research is being advanced. One of the typical polysaccharides in the cell wall, which is a component of yeast, is β-glucan, and it is said to have effects on skin moisturization, anti-aging, and allergies, so it is also widely used as supplements, food modifiers, cosmetics, etc.

[0003] β-glucan is a dietary fiber in which D-glucose is linked by β-glycosidic bonds. β-glucan derived from yeast has a structure in which long branches via β-1,6 bonds are mixed in a linear β-1,3 bond of 30 residues and shows water-insolubility (Non-Patent Document 1).

[0004] As a method for preparing high-purity β-glucan from yeast, a method of culturing black yeast and producing it outside the cells is known (Patent Document 1).

[0005] Further, Patent Document 2 presents a method of hydrolyzing brewer's yeast with an aqueous acid solution or an aqueous alkali solution and then recovering a glucan-containing composition from the hydrolyzate.

[0006] Patent Document 3 presents a method of extracting an extract component from yeast and extracting β-glucan from the residue component.

[0007] Furthermore, Patent Document 4 presents a powder composition of a yeast-derived material, etc.

Prior Art Documents

Patent Documents

[0008]

Patent Document 1

[0009] [Non-Patent Document 1] Journal of the Brewing Society of Japan, Vol. 104, No. 12, 6F p. 939-943 (December 2009) [Summary of the Invention] [Problems to be Solved by the Invention]

[0010] When using yeast-derived β-glucan as a filler, there were problems in the conventional methods, such as it being water-soluble and difficult to use, having non-uniform particle sizes, or containing proteins, which limited it to a very low addition rate.

[0011] In view of the above situation, as a result of intensive research, the present inventors have found that it is possible to produce yeast-derived cell wall microparticles that are excellent in various aspects such as the filler effect, and have thus completed the present invention described below.

[0012] [1] A method for producing yeast cell wall particles, comprising (A) to (D): (A): After treating yeast cells or yeast residue after yeast extract extraction with NaOH, performing solid-liquid separation; (B): After treating the liquid-depleted cake obtained in (A) with NaClO, performing solid-liquid separation; (C): Concentrating the liquid-depleted cake obtained in (B); and (D): Spray-drying the concentrated cake obtained in (C) to obtain yeast cell wall particles. [2] The production method according to claim 1, wherein in (A) and (B), a liquid-depleted cake from which components mainly composed of proteins have been extracted is obtained. 〔3〕The production method according to claim 1 or 2, wherein solid separation is performed by a disk-type separator in (A) and (B), or (A) or (B). 〔4〕The production method according to any one of claims 1 to 3, wherein the β-glucan content of the yeast cell wall particles is 30% or more. 〔5〕The production method according to any one of claims 1 to 3, wherein the protein content is 1.5% or less. 〔6〕The production method according to any one of claims 1 to 3, wherein the yeast cells or the yeast cells after yeast extract extraction are Candida utilis. 〔7〕A cosmetic composition comprising yeast cell wall particles obtained by the production method according to any one of claims 1 to 6 as an active ingredient.

Advantages of the Invention

[0013] According to the present invention, it is possible to provide yeast cell wall microparticles as a uniform filler with good skin feel mainly composed of β-glucan while maintaining the shape of yeast. Further, in the present invention, the protein content that causes allergy is extracted until it reaches the lower limit, and it is useful as a cosmetic.

Embodiments for Carrying Out the Invention

[0014] The present invention relates to a method for producing yeast cell wall microparticles. Specifically, after treating yeast cells or yeast cells after yeast extract extraction with NaOH, the liquid-depleted cake obtained by solid-liquid separation is treated with NaClO, and the liquid-depleted cake obtained by solid-liquid separation again is concentrated and spray-dried to obtain yeast cell wall particles. Hereinafter, embodiments of the present invention will be described. Unless otherwise specified, the description of "AA~BB" for a numerical range indicates "AA or more and BB or less" (where "AA" and "BB" represent arbitrary numerical values).

[0015] (Yeast cell wall particles) The cosmetic composition of the present invention contains yeast cell wall particles. In the present invention, the term "yeast cell wall particles" means particles formed of the cell wall of yeast.

[0016] The yeast cell wall particles used in the present invention maintain the shape of the yeast cell wall. "Maintaining the shape of the yeast cell wall" means that the cell wall is not in a damaged state where it is broken or crushed and cannot retain the outer shape of the yeast. Instead, when looking at the overall shape of the particles, the cell wall maintains an outer shape that is the same as or close to the overall shape of live yeast cells. Therefore, the yeast cell wall particles used in the present invention have an outer shape that is substantially the same as or similar to that of live yeast cells.

[0017] As an index for whether the cell wall of the yeast cell wall particles maintains its shape, the average particle size of the yeast cell wall particle diameter can be used. The upper limit of the average particle size of the yeast cell wall particles used in the present invention is 25 μm or less, preferably 20 μm or less, and more preferably 15 μm or less. On the other hand, the lower limit of the average particle size is 1 μm or more, preferably 3 μm or more, and more preferably 5 μm or more. If the chemical or physical treatment (detailed below) for removing intracellular components of yeast is too strong, yeast cells tend to aggregate, the outer shape of the yeast cannot be maintained, and the average particle size of the yeast cell wall particles tends to be outside the above upper and lower limits.

[0018] The average particle size shown in the present invention can be determined, for example, by using a laser diffraction particle size distribution measuring device (e.g., Mastersizer 3000, manufactured by Malvern), using water as the dispersion medium for measurement, adding about 0.1 g of the sample for measurement, and obtaining the cumulative volume 50% particle size as the average particle size.

[0019] By maintaining the shape of the yeast cell wall and incorporating yeast cell wall particles having the above-described average particle diameter into a cosmetic composition, a cosmetic composition with a high soft focus effect can be obtained. Although its mechanism of action is not necessarily clear, it is presumed that the predetermined shape and size as described above contribute to producing favorable light scattering. Also, since yeast cell wall particles are natural materials, there are variations due to individual differences in the size of each yeast (for example, the average particle diameter), and it is also presumed that an appropriate variation in the size of the yeast cell wall particles contributes to producing favorable light scattering. Further, by maintaining the shape of the yeast cell wall and incorporating yeast cell wall particles having the above-described average particle diameter into a cosmetic composition, it is presumed that unevenness and wrinkles on the skin can be moderately filled, dullness caused by shadows can be suppressed, and a cosmetic composition with a high beautifying effect can be obtained.

[0020] In the present invention, the yeast cell wall particles preferably have an oval planar shape. Here, the "planar shape" means the two-dimensional shape when the object is viewed from the normal direction of the plane (i.e., the tangent plane) in contact with the object. Therefore, in the present invention, the planar shape of the yeast cell wall particles means the two-dimensional shape when the yeast cell wall particles are viewed from the normal direction of at least one tangent plane (for example, one tangent plane parallel to the major axis direction of the particle) in contact with the outermost shell portion of the yeast cell wall particles. That is, as the yeast cell wall particles of the present invention, those having an oval planar shape when viewed from the normal direction of at least one tangent plane (for example, one tangent plane parallel to the major axis direction of the particle) in contact with the outermost shell portion of the yeast cell wall particles are preferred.

[0021] In the present invention, the term "oval shape" means an ellipse or a curved shape close thereto, and includes an oval shape, a rice grain shape, an oblong shape, and the like. Also, the "oval shape" may include a straight line portion in a part of the curved shape, and for example, a rounded rectangle is also included. As the oval-shaped yeast cell wall particles used in the present invention, it is preferable that there is at least one axis of symmetry that is symmetric about the left and right in the oval planar shape, but it may have a non-axisymmetric shape without a geometrically exact axis of symmetry.

[0022] The sphericity of the yeast cell wall particles used in the present invention usually falls within the range of 0.1 or more and less than 1.0, preferably 0.2 or more and less than 1.0, more preferably 0.4 or more and less than 1.0, due to the retention of the yeast cell wall.

[0023] Since the yeast cell wall particles are non-spherical, having an oval planar shape or adjusted to an appropriate range such as sphericity, the soft focus effect can be enhanced more. Although the mechanism of action is not necessarily clear, it is presumed that having such a shape contributes to producing preferable light scattering.

[0024] (Yeast raw material) The yeast raw material is the yeast residue of yeast belonging to the genus Candida. Candida yeast is not particularly limited, and examples include Candida utilis (Cyberlindnera jadinii; Torula yeast), Candida kefyr, Candida etchellsiii, Candida stellata, Candida versatilis, etc., and it is preferable to include Candida utilis.

[0025] (Yeast residue) In this specification, the yeast residue is obtained by removing at least a part of the yeast cells from the yeast culture, and preferably, it is the residue after extracting yeast extract from the yeast culture. The yeast residue usually contains components derived from the cell wall. Examples of the method for extracting yeast extract include autolysis method, hot water extraction method, enzyme extraction method, acid-alkali extraction method, and the hot water extraction method and enzyme extraction method are preferable, and the enzyme extraction method (for example, extraction using a proteolytic enzyme (protease)) is more preferable. The yeast residue may be of one type or a combination of two or more yeast residues with different yeast types and extraction methods.

[0026] (Preparation of dewatered cake) In this specification, the above-mentioned de-liquefied cake powder can be manufactured by a first treatment step, a solid-liquid separation step, a second treatment step, and a solid-liquid separation step. Each step will be described below.

[0027] [First Treatment Step] In the first treatment step, yeast residues of Candida yeast are suspended in an aqueous solvent, and NaOH is added to a concentration of 0.1 to 5%. Examples of the method of suspending the yeast residues in the aqueous solvent include adding the aqueous solvent to the yeast residues and stirring as necessary. Stirring is usually carried out for 10 minutes or more, preferably 30 minutes or more, more preferably 2 hours or more, and even more preferably 3 hours or more while dispersing using a stirrer (for example, at 300 rpm or less, 250 rpm or less, 200 rpm or less). The upper limit is not particularly limited, but it is usually within 10 hours. The temperature conditions can be set, for example, at 50°C to 90°C (preferably 70 to 75°C).

[0028] [Solid-Liquid Separation Step (First Washing)] In the solid-liquid separation step (first washing), after dilution with water, a de-liquefied cake can be obtained, for example, by centrifugation. The centrifugation can be carried out one or more times, preferably two or more times, and more preferably three or more times.

[0029] [Second Treatment Step] In the second treatment step, the de-liquefied cake obtained by the above-mentioned solid-liquid separation is suspended in an aqueous solvent, and NaClO is added to a concentration of 0.01 to 0.5%. Examples of the method of suspending the yeast residues in the aqueous solvent include adding the aqueous solvent to the yeast residues and stirring as necessary. Stirring is usually carried out for 1 hour or more, preferably 5 hours or more, more preferably 10 hours or more, and even more preferably 18 hours or more while dispersing using a stirrer (for example, at 300 rpm or less, 250 rpm or less, 200 rpm or less). The upper limit is not particularly limited, but it is usually within 30 hours. The temperature conditions can be set, for example, at 10°C to 40°C (preferably 20 to 30°C, more preferably 25 to 28°C).

[0030] [Solid-Liquid Separation Step (Second Washing)] In the solid-liquid separation step (second washing), a de-liquefied cake can be obtained, for example, by centrifugation after dilution with water. The centrifugation can be performed one or more times, preferably two or more times, and more preferably three or more times.

[0031] [Drying step] For example, but not limited to this, it can be produced by drying the above-mentioned de-liquefied cake. The dispersion medium in the dispersion liquid to be dried is not particularly limited, but water is preferred. The device used for drying is not particularly limited. For example, a vacuum drum dryer, an atmospheric pressure drum dryer, a spray dryer, a hot air dryer, a warm air dryer, etc. can be used.

[0032] [Total protein] It is desirable that the protein be removed from the yeast cell wall microparticles. The total protein content in the yeast cell wall microparticles is preferably 5% by weight or less, more preferably 2% by weight or less, and even more preferably 1% by weight or less.

[0033] [β-glucan] The content of β-glucan is preferably 20% by weight or more, more preferably 30% by weight or more, and even more preferably 40% by weight or more.

[0034] Yeast cell wall fraction particles, which are a preferred form of yeast cell wall particles, have a higher β-glucan content and are more excellent in terms of moisture retention. Also, since cytoplasmic matrices and organelles are removed, they are more excellent in terms of storage stability, etc. as a component to be blended in a cosmetic composition.

[0035] In addition, the above-mentioned yeast cell wall particles can also be used as a powder composition for enhancing the soft focus effect in technical fields other than cosmetic applications.

[0036] [Cosmetic composition] The cosmetic composition of the present invention contains the above-mentioned yeast cell wall particles. The cosmetic composition of the present invention can be obtained, for example, by blending the yeast cell wall particles into the cosmetic composition and stirring and mixing them.

[0037] The blending amount of the yeast cell wall particles in the cosmetic composition of the present invention can be appropriately adjusted according to the use, dosage form, etc. of the cosmetic composition. From the viewpoint of the effects of the present invention, the upper limit of the blending amount of the yeast cell wall particles is not particularly limited, but generally, as the upper limit when mixing powders in cosmetics, for example, it can be 90% by weight or less, 70% by weight or less, or 50% by weight or less. Further, in the case of a cosmetic in which a component other than the yeast cell wall particles serves as the main dispersion medium, from the viewpoint of sufficient dispersion, the upper limit of the blending amount is preferably 30% by weight or less, more preferably 25% by weight or less, and still more preferably 20% by weight. On the other hand, the lower limit of the blending amount of the yeast cell wall particles is preferably 5% by weight or more, more preferably 10% by weight or more, and still more preferably 15% or more. By setting the lower limit of the blending amount in this way, a cosmetic composition excellent in soft focus effect, skin beautifying effect, and moisture retention can be obtained.

[0038] Since the yeast cell wall particles have the above-described characteristics, the cosmetic composition of the present invention is suitable as a makeup cosmetic. The makeup cosmetic may be either a base makeup cosmetic or a point makeup cosmetic. Examples of the base makeup cosmetic include powder makeup products such as foundation and face powder. Examples of the point makeup cosmetic include lipsticks, blushes, eyeliners, mascaras, eyeshadows, eyebrows, nail enamels, and nail treatments.

[0039] Preferred dosage forms of the makeup cosmetic according to the present invention include, for example, powder cosmetics. By using a powder cosmetic such as loose powder, there is no need to perform an emulsification step or press molding, so there is no need to limit the blending amount of the yeast cell wall particles for the purpose of maintaining the shape, and the desired effects can be easily obtained.

[0040] The cosmetic composition of the present invention contains yeast cell wall particles, but other powders generally used as cosmetic ingredients may be blended according to conditions such as the use of the cosmetic composition. Examples of powders for cosmetics include inorganic powders, lustrous powders, organic powders, pigment powders, metal powders, composite powders, and the like.

[0041] More specifically, the following are exemplified. Examples of white inorganic pigments include titanium oxide, zinc oxide, cerium oxide, barium sulfate, talc, muscovite, phlogopite, biotite, synthetic mica, sericite, synthetic sericite, kaolin, silicon carbide, smectite, aluminum oxide, magnesium oxide, zirconium oxide, antimony oxide, diatomaceous earth, aluminum silicate, magnesium aluminum metasilicate, calcium silicate, barium silicate, magnesium silicate, calcium carbonate, magnesium carbonate, hydroxyapatite, boron nitride, silicon dioxide, and the like.

[0042] Examples of colored inorganic pigments include red iron oxide, yellow iron oxide, black iron oxide, carbon black, black titanium oxide, chromium oxide, chromium hydroxide, ultramarine blue, and ultramarine.

[0043] Examples of lustrous powders include titanium dioxide-coated mica, titanium dioxide-coated synthetic phlogopite, titanium dioxide-coated bismuth oxychloride, iron oxide-coated mica, iron oxide-coated mica titanium, ultramarine-treated mica titanium, carmine-treated mica titanium, bismuth oxychloride, fish scale foil, titanium dioxide-coated glass powder, aluminum powder, and the like.

[0044] Examples of the organic polymer resin powder include lamellar agents of resin laminate powders such as polyethylene terephthalate-aluminum-epoxy laminate powder, polyethylene terephthalate-polyolefin laminate film powder, polyethylene terephthalate-polymethyl methacrylate laminate film powder, polyethylene terephthalate-aluminum-epoxy laminate powder, etc.; furthermore, copolymer resins such as polyamide resins, polyethylene resins, polyacrylic resins, polyester resins, fluorine resins, cellulose resins, polystyrene resins, styrene-acrylic copolymer resins, polypropylene resins, silicone resins, urethane resins, etc. can be mentioned.

[0045] Examples of the organic low molecular weight powder include N-acyl lysine, etc.

[0046] Examples of the natural organic powder include starch, silk powder, cellulose powder, etc.

[0047] Examples of the organic pigment powder include Red No. 3, Red No. 104, Red No. 106, Red No. 201, Red No. 202, Red No. 205, Red No. 226, Red No. 228, Orange No. 203, Orange No. 204, Orange No. 205, Blue No. 1, Blue No. 404, Yellow No. 4, Yellow No. 5, Yellow No. 401, Green No. 3, etc.

[0048] Examples of the metal powder include aluminum powder, gold powder, silver powder, etc.

[0049] Examples of the composite powder include fine particle titanium oxide-coated mica titanium, fine particle zinc oxide-coated mica titanium, barium sulfate-coated mica titanium, titanium dioxide-containing silicon dioxide, zinc oxide-containing silicon dioxide, etc.

[0050] In addition, the above powders may be surface-treated using one or more selected from fluorinated compounds, silicone compounds, metal soaps, lecithin, hydrogenated lecithin, collagen, hydrocarbons, higher fatty acids, higher alcohols, esters, waxes, rosin, surfactants, etc.

[0051] In addition to the above yeast cell wall particles, the cosmetic composition of the present invention can be prepared by appropriately adding oil components, emulsifiers, etc., which are generally used as cosmetics, according to the target product.

[0052] Examples of the oil components used in cosmetics include hydrocarbons such as liquid paraffin and squalane; natural animal and plant fats and oils such as olive oil, jojoba oil, avocado oil, soybean oil, meadowfoam oil, and lanolin; fatty acid esters such as cetyl 2-ethylhexanoate, isononyl isononanoate, isotridecyl isononanoate, isopropyl myristate, isopropyl palmitate, 2-ethylhexyl palmitate, 2-ethylhexyl stearate, and stearyl stearate; triglycerides such as glyceryl tri(caprylyl / caprate) and glyceryl tri(2-ethylhexanoate), and polyhydric alcohol fatty acid ester oils; higher alcohols such as cetyl alcohol, stearyl alcohol, isostearyl alcohol, and behenyl alcohol; higher fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and isostearic acid; chain silicone oils such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic silicone oils such as decamethylcyclopentasiloxane, and liquid or solid silicone oils such as trimethylsiloxysilicate, silicone gel, and silicone powder. These oil components can be used alone or in combination of two or more.

[0053] Examples of emulsifiers used in cosmetics include, for example, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, and silicone-based surfactants as emulsifiers for silicone oils. Preferably, nonionic surfactants and silicone-based surfactants are used, and one or more of these can be used in combination. Examples of nonionic surfactants include polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, and their derivatives; sorbitan fatty acid esters such as sorbitan monopalmitate, sorbitan monostearate, sorbitan monooleate, sorbitan coconut fatty acid ester, sorbitan tristearate, sorbitan trioleate; polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan tristearate, polyoxyethylene sorbitan trioleate; polyoxyethylene sorbit fatty acid esters such as polyoxyethylene sorbit monolaurate, polyoxyethylene sorbit tetra-stearate fatty acid ester; glycerin fatty acid esters such as glyceryl monostearate, self-emulsifying glyceryl monostearate, glyceryl monoisostearate; polyoxyethylene glyceryl fatty acid esters such as polyoxyethylene glyceryl monostearate, polyoxyethylene glyceryl monoisostearate, polyoxyethylene glyceryl tristearate, polyoxyethylene glyceryl triisostearate, polyoxyethylene glyceryl trioleate; polyoxyethylene alkyl ethers such as polyoxyethylene lauryl ether, polyoxyethylene cetyl ether, polyoxyethylene stearyl ether, polyoxyethylene oleyl ether, polyoxyethylene octyldodecyl ether; polyethylene glycol fatty acid esters such as polyethylene glycol monolaurate, polyethylene glycol monooleate; polyglycerol fatty acid esters such as polyglycerol monolaurate, polyglycerol monomyristate, polyglycerol monostearate, polyglycerol monooleate, polyglycerol tristearate, polyglycerol trioleate, etc.Examples of silicone surfactants include polyether-modified silicone, polyglycerin-modified silicone, and the like.

[0054] In addition to the above, the cosmetic composition of the present invention may contain components generally used as cosmetics. For example, the cosmetic composition of the present invention may contain polyhydric alcohols, moisturizers, saccharides, preservatives, antibacterial agents, sequestering agents, polymeric thickeners such as water-soluble polymers, lower alcohols, film-forming agents, neutralizing agents, pH adjusters, powder components, or ultraviolet absorbers, etc. Further, the cosmetic composition of the present invention may contain, for example, other cosmetic components, medicinal components, physiologically active components, fragrances, and pigments such as vitamins, skin activators, blood circulation promoters, anti-inflammatory agents, whitening agents, or anti-wrinkle components.

[0055] Examples of polyhydric alcohols include 1,3-butylene glycol, dipropylene glycol, propylene glycol, glycerin, 1,2-pentanediol, isoprene glycol, polyethylene glycol, methyl glucoside, sorbitol, diglycerin, and the like.

[0056] Examples of moisturizers include hyaluronic acid, collagen, elastin, sodium lactate, cyclodextrin, pyrrolidone carboxylic acid and its salts, natural and synthetic ceramides, and the like.

[0057] Examples of preservatives and antibacterial agents include benzoic acid, salicylic acid, phenol, sorbic acid, paraoxybenzoic acid esters, parachlorometacresol, hexachlorophene, benzalkonium chloride, chlorhexidine chloride, trichlorocarbanilide, photosensitizers, phenoxyethanol, and the like.

[0058] Examples of sequestering agents include edetate salts such as disodium ethylenediaminetetraacetate, edetic acid, and sodium edetate.

[0059] Examples of the water-soluble polymer or the viscosity modifier include gum arabic, tragacanth gum, galactan, guar gum, carrageenan, pectin, agar, quince seed, dextran, dextrin, pullulan, carboxymethyl starch, collagen, casein, gelatin, methyl cellulose, methylhydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, sodium carboxymethyl cellulose, sodium alginate, sodium carboxymethyl dextran, carboxyvinyl polymer, bentonite and the like.

[0060] Examples of the film-forming agent include alkyl polyacrylate, eicosene-vinylpyrrolidone polymer, ester gum and the like.

[0061] Examples of the neutralizing agent include potassium hydroxide, sodium hydroxide, triethanolamine, sodium carbonate and the like.

[0062] Examples of the pH adjuster include lactic acid, citric acid, glycolic acid, succinic acid, tartaric acid, dl-malic acid, potassium carbonate, sodium bicarbonate, ammonium bicarbonate and the like.

[0063] Examples of the lower alcohol include ethanol, isopropanol and the like.

[0064] Examples of the ultraviolet absorber include cinnamic acid-based ultraviolet absorbers such as 2-ethylhexyl p-methoxycinnamate, hexyl 2-(4-diethylamino-2-hydroxybenzoyl) benzoate, benzyl p-methoxycinnamate, 2-ethylhexyl p-methoxycinnamate, 2-ethoxyethyl p-methoxycinnamate, glyceryl monopara-methoxycinnamate mono-2-ethylhexanoate, isopropyl p-methoxycinnamate·diisopropyl cinnamate ester mixture; benzophenone-based ultraviolet absorbers such as hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid, sodium hydroxymethoxybenzophenone sulfonate, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone; benzoic acid ester-based ultraviolet absorbers such as para-aminobenzoic acid, ethyl para-aminobenzoate, glyceryl para-aminobenzoate, amyl para-dimethylaminobenzoate, octyl para-dimethylaminobenzoate, ethyl 4-[N,N-bis(2-hydroxypropyl)amino]benzoate, hexyl 2-(4-diethylamino-2-hydroxybenzoyl)-benzoate; salicylic acid-based ultraviolet absorbers such as ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, p-tert-butylphenyl salicylate, homomenthyl salicylate; triazine-based ultraviolet absorbers such as 2,4,6-trianiolino-p-(carbo-2'-ethylhexyl-1'-oxy)-1,3,5-triazine; and others such as 4-tert-butyl-4'-methoxydibenzoylmethane, oxybenzone, octocrylene, menthyl anthranilate, 2-(2-hydroxy-5-methylphenyl)benzotriazole, 2-ethylhexyl dimethoxybenzylidene dioxoimidazolidine propionate, 2-phenylbenzimidazole-5-sulfonic acid, 2-cyano-3,3-diphenylacrylate.

[0065] Examples of the active ingredient include the following. As vitamins, for example, vitamin A such as coenzyme Q10, vitamin A oil, retinol, etc.; vitamin B2 such as riboflavin; B6 such as pyridoxine hydrochloride; vitamin C such as L-ascorbic acid, magnesium L-ascorbate phosphate, L-ascorbic acid monopalmitate, L-ascorbic acid dipalmitate, L-ascorbic acid-2-glucoside, etc.; pantothenic acids such as calcium pantothenate, vitamin D such as vitamin D2, cholecalciferol, etc.; vitamin E such as α-tocopherol, tocopherol acetate, DL-α-tocopherol nicotinate, etc. can be mentioned.

[0066] As skin whitening agents, for example, arbutin, ellagic acid, tranexamic acid, placenta extract, glutathione, yukinoshita extract, etc. can be mentioned.

[0067] As skin activators, for example, royal jelly, buna no ki extract, etc. can be mentioned.

[0068] As blood circulation promoters, for example, capsaicin, gingerol, cantharidin tincture, ichthyol, caffeine, tannic acid, γ-oryzanol, etc. can be mentioned.

[0069] As anti-inflammatory agents, for example, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, azulene, allantoin, etc. can be mentioned.

[0070] As amino acids, for example, arginine, serine, leucine, tryptophan, etc. can be mentioned.

[0071] Furthermore, as various extracts, for example, Japanese snowbell seed extract, chamomile flower extract, white peony root extract, button extract, parsley extract, beech tree extract, wine yeast extract, grapefruit extract, passionflower extract, rice extract, grape extract, hop extract, rice bran extract, loquat extract, magnolia bark extract, ginseng extract, schizandra extract, meadowsweet extract, peach extract, licorice extract, peony extract, soapwort extract, loofah extract, capsicum extract, lemon extract, gentian extract, perilla extract, aloe extract, rosemary extract, sage extract, cinnamon extract, thyme extract, tea extract, seaweed extract, cucumber extract, clove extract, carrot extract, marronnier extract, witch hazel extract, mulberry extract, okgon extract, houttuynia extract, Japanese hawthorn extract, sedum extract, kudzu root nodule extract, artichoke leaf extract, polygonatum extract, edelweiss extract, etc. can be mentioned.

[0072] Preferable types of the makeup cosmetics of the present invention include, for example, foundation and makeup base. The dosage form of the foundation can be, for example, a loose foundation, a cake powder foundation, a W / O emulsified liquid foundation, an oil stick foundation, etc. The dosage form of the makeup base can be a W / O emulsified makeup base, an O / W emulsified makeup base, etc.

[0073] Since the makeup cosmetics of the present invention contain yeast cell wall particles, they have excellent soft focus effect, beauty effect, and moisturizing effect, which are particularly expected effects in makeup cosmetics. Further, the makeup cosmetics of the present invention can have yeast cell wall particles as the main component of the powder component, are excellent in safety, and can be made into makeup cosmetics with reduced environmental load.

Examples

[0074] The present invention will be specifically described below with reference to examples, but the technical scope of the present invention is not limited to the following examples. <Example 1> As follows, the cell wall fraction of yeast from which intracellular components were removed was decolorized, defatted, and dried to prepare yeast cell wall fraction particles.

[0075] 3000 g of KR yeast was dissolved in 30 L of water, 625 g of a 48% sodium hydroxide aqueous solution was added, and a decolorization reaction was carried out with stirring for 3 hours in a water bath adjusted to 75°C. After the reaction, 170 L of water was added and diluted 6-fold, and then 30 L of the heavy liquid discharged at 1-minute intervals by passing through at 380 L / Hr was recovered using a disk-type separation separator (BTPX205 type) manufactured by Alfa Laval. Thereafter, 170 L of water was added again and diluted 6-fold, and the operation of separating using the disk-type separation separator (BTPX205 type) manufactured by Alfa Laval was repeated twice. After adjusting the pH of the recovered 30 kg of heavy liquid (yeast concentration was 3.1%) to 6.8 with concentrated hydrochloric acid, 499 g of a 48% sodium hydroxide aqueous solution and 3 L of 4% sodium hypochlorite were added, and a reaction was carried out at 28°C for 18 hours. Thereafter, the heavy liquid was recovered under the same conditions using the above separation separator. The recovered heavy liquid was adjusted to pH = 6.8 with concentrated hydrochloric acid and then dried using a spray dryer (manufactured by Tokyo Rika Kikai Co., Ltd.) to obtain a white powder.

[0076] <Example 2> A white powder was obtained in the same manner as in Example 1 except that GL yeast was used instead of KR yeast.

[0077] <Comparative Example 1> A white powder was obtained in the same manner as in Example 1 except that the decolorization reaction with stirring for 3 hours in a water bath adjusted to 75°C by adding 625 g of a 48% sodium hydroxide aqueous solution was omitted.

[0078] <Evaluation Method> The following items were evaluated for the white powders (yeast cell wall fraction particles) obtained in Examples 1 and 2 and Comparative Example 1.

[0079] <Yield> The weight of the residue (yeast cell wall fraction sample) at the stage before spray drying was divided by the viable cell weight (charged yeast weight) of the yeast used as the raw material and expressed as a percentage to obtain the yield (%).

[0080] <Particle size distribution and average particle diameter> A laser diffraction particle size distribution analyzer (MasterSizer 3000, manufactured by Malvern) was used. The dispersion medium used for the measurement was water. 0.1 g of the sample was added and the measurement was carried out to obtain the particle size distribution and the cumulative deposition 50% particle diameter (average particle diameter).

[0081] <Protein> The nitrogen content was measured by the Kjeldahl method, and the total protein amount was calculated by multiplying the measured value by a coefficient of 6.25.

[0082] <β-glucan content> The β-glucan content was measured using a β-glucan measurement kit (manufactured by Megazyme) according to the attached manual.

[0083] The results of each item measured for the white powders of Examples 1 to 2 and Comparative Example 1 according to the above measurement methods are shown in the following table. TIFF2025090169000001.tif25157

Claims

1. A method for producing yeast cell wall particles, comprising (A) to (D): (A): After treating yeast cells or yeast residue after yeast extract extraction with NaOH, performing solid-liquid separation; (B): After treating the liquid-depleted cake obtained in (A) with NaClO, performing solid-liquid separation; (C): Concentrating the liquid-depleted cake obtained in (B); and (D): Spray-drying the concentrated cake obtained in (C) to obtain yeast cell wall particles.

2. The production method according to claim 1, wherein in (A) and (B), a liquid-depleted cake from which components mainly composed of proteins have been extracted is obtained.

3. The production method according to claim 1 or 2, wherein in (A) and (B), (A) or (B), solid separation is performed by a disk-type separator.

4. The production method according to any one of claims 1 to 3, wherein the β-glucan content of the yeast cell wall particles is 30% or more.

5. The production method according to any one of claims 1 to 3, wherein the protein content is 1.5% or less.

6. The production method according to any one of claims 1 to 3, wherein the yeast cells or the yeast cells after yeast extract extraction are Candida utilis.

7. A cosmetic composition comprising yeast cell particles obtained by the production method according to any one of claims 1 to 6 as an active ingredient.

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