Estimation method, estimation device and estimation program of skin condition of subject, and proposal method, proposal device and proposal program of composition and / or stimulation suitable for subject (excluding medical treatment)

Abstract

To provide a novel method for estimating a skin condition of a subject by using skin.SOLUTION: A method for estimating a skin condition of a subject has: a gene expression analysis step of analyzing gene expression on skin of a subject; and a skin condition estimation step of estimating a skin condition of the subject on the basis of a gene expression analysis result of the subject and an expression level of a gene related to a specific skin condition, where the gene related to the specific skin condition is a gene in which a plurality of persons is classified into a group having a specific skin condition and a group which does not have a specific skin condition and an increase / decrease of an expression level is recognized in both groups.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The present invention relates to a method for estimating the skin condition of a subject, an estimation device, an estimation program, and a method for proposing a composition and / or a stimulus (excluding medical acts) suitable for the subject, a proposal device, and a proposal program.

Background Art

[0002] Conventionally, in order to estimate the skin condition of a subject, many studies have been conducted on the relationship between the skin condition and factors. For example, Patent Document 1 describes a method for evaluating skin pigmentation, characterized by performing gene expression analysis using keratin collected from the skin of a test subject and evaluating skin pigmentation based on the results, wherein the gene is the NT5E gene.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] In view of the above prior art, an object of the present invention is to provide a novel method for estimating the skin condition of a subject using the skin. Another object of the present invention is to provide a novel method for proposing a composition and / or a stimulus (excluding medical acts) suitable for the subject based on the estimated skin condition of the subject.

Means for Solving the Problems

[0005] The present invention for solving the above problems is a gene expression analysis step of analyzing the expression of genes in the skin of a subject, A skin condition estimation step of estimating the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of genes related to a specific skin condition having The genes related to the specific skin condition are genes in which an increase or decrease in expression level was observed in both groups when a plurality of people were classified into a group having the specific skin condition and a group not having the specific skin condition A method for estimating the skin condition of a subject

[0006] In a preferred form of the present invention, the genes related to the specific skin condition are genes having one or more gene ontologies selected from one or more groups selected from the following groups A to F

[0007] [Group A] [Table 1]

[0008] [Group B] [Table 2]

[0009] [Group C] [Table 3]

[0010] [Group D] [Table 4]

[0011] [Group E] [Table 5]

[0012] [Group F] [Table 6]

[0013] In a preferred embodiment of the present invention, the gene related to the specific skin condition is a gene having one or more gene ontologies selected from one or more groups selected from the following groups G to L.

[0014] [Group G] [Table 7]

[0015] [Group H] [Table 8]

[0016] [Group I] [Table 9]

[0017] [Group J] [Table 10]

[0018] [Group K] [Table 11]

[0019] [Group L] [Table 12]

[0020] In a preferred embodiment of the present invention, the gene related to the specific skin condition is one or more genes selected from the following groups a to k. [Group a] MMP1, MMP3, CCL21, COL1A1, COL3A1, ELN, CLASP2, COMP, DAG1, FAP, SERPINB5, ELANE, LY6G5B, KRT10, IVL [Group I] MMP1, MMP3, CCL21, CXCR3, TNF, CCR2, RIPK2, AIPL1, ELANE, OGN, COL1A1, COL3A1, LY6G5B [Group U] IGF1, IGFBP3, IL1A, KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, PLIN2 [Group E] FLG, IL4, IL13, KDF1, KRT16, GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, ARTN [Group O] FLG, CCL17, IL4, IL13, CDH1, CLDN1, KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, LORICRIN [Group K] CASP1, CASP4, CD44, CDH5, COL6A1, ELANE, EGFR, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, TRPV1, USP50

[0021] In a preferred embodiment of the present invention, the gene related to the specific skin condition is one or more genes selected from the following Groups Ki to Shi. [Group Ki] SMAD3, TGFB1, MMP2, MMP9, MMP13, FOS, JUN, RFX5, IRF1, STAT1, BMP2, STAT3 [Group Ku] ELN, MMP12, MME, CSF2 [Group Ke] ALOX15, SLC27A1, SREBF1, DGAT1, FASN, ELF4, HMGCR [Group Ko] IL1B, TLR2, CXCL8, CXCL2, LCN2, STAT1, IFNG, IRF1, IL17A, TNF, TRPV1, TRPV2, TRPV3, TRPV4, TRPM2, TRPM4, TRPC6 [Group S] MMP12, IL1A, IL1B, IL36G, IFNG, CXCL10, STAT3, TSLP, STAT1, CXCL8, IL4R, CCL18, CCL2, IL17A, IL23A, S100A7, S100A8, S100A9, S100A12, JUN, TNF, FOS [Group Sh] CD14, LY96, TNFSF13B, JUN, EIF2AK2, TREM1

[0022] In a preferred embodiment of the present invention, the gene related to the specific skin condition is a gene in which an increase or decrease in the expression level is observed in both groups, classified based on face images of a plurality of persons, responses to questionnaires for skin evaluation obtained from a plurality of persons, and / or diagnosis results by doctors of a plurality of persons into a group having a specific skin condition and a group not having a specific skin condition.

[0023] In a preferred embodiment of the present invention, the skin condition is one or more selected from wrinkles, sagging, enlarged pores, sensitive skin, atopic dermatitis, rosacea, red face, and acne.

[0024] In a preferred embodiment of the present invention, the estimating step is to determine the skin condition as Wrinkles caused by fluctuations in gene expression having one or more gene ontologies selected from the above Group A, Sagging caused by fluctuations in gene expression having one or more gene ontologies selected from the above Group B, Enlarged pores caused by fluctuations in gene expression having one or more gene ontologies selected from the above Group C, sensitive skin caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group D, atopic dermatitis caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group E, and / or acne caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group F including the step of presuming that it is so.

[0025] In a preferred form of the present invention, the presuming step comprises presuming the skin condition as wrinkles caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group G, flabbiness caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group H, enlarged pores caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group I, sensitive skin caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group J, atopic dermatitis caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group K, and / or acne caused by fluctuations in gene expression having one or more Gene Ontologies selected from the group L including the step of presuming that it is so.

[0026] In a preferred form of the present invention, the presuming step comprises presuming the skin condition as wrinkles caused by fluctuations in gene expression of one or more genes selected from the group alpha, Wrinkles caused by fluctuations in the expression of one or more genes selected from the group I, Enlarged pores caused by fluctuations in the expression of one or more genes selected from the group U, Sensitive skin caused by fluctuations in the expression of one or more genes selected from the group E, Atopic dermatitis caused by fluctuations in the expression of one or more genes selected from the group O, And / or acne caused by fluctuations in the expression of one or more genes having one or more gene ontologies selected from the group Ka, It is estimated to be.

[0027] In a preferred embodiment of the present invention, the estimating step comprises estimating the skin condition, Wrinkles caused by fluctuations in the expression of one or more genes selected from the group Ki, Wrinkles caused by fluctuations in the expression of one or more genes selected from the group Ku, Enlarged pores caused by fluctuations in the expression of one or more genes selected from the group Ke, Sensitive skin caused by fluctuations in the expression of one or more genes selected from the group Ko, Atopic dermatitis caused by fluctuations in the expression of one or more genes selected from the group Sa, And / or acne caused by fluctuations in the expression of one or more genes having one or more gene ontologies selected from the group Shi, It is estimated to be.

[0028] In a preferred embodiment of the present invention, the gene expression analysis is performed by PCR or transcriptome analysis using RNA extracted from the skin as a sample.

[0029] In a preferred embodiment of the present invention, the volume of the skin immediately after being obtained from the subject is 0.05 mm 3 Or less.

[0030] In addition, the present invention includes a gene expression analysis step of analyzing the gene expression in the skin of a subject, a skin condition estimation step of estimating the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition, a proposal step of proposing a composition and / or a stimulus (excluding medical treatments) suitable for the subject based on the estimated skin condition, and has the gene related to the specific skin condition is a gene in which the increase or decrease in the expression level was observed in both groups when a plurality of persons were classified into a group having a specific skin condition and a group not having a specific skin condition, It is also a method for proposing a composition and / or a stimulus (excluding medical treatments) suitable for a subject.

[0031] In addition, the present invention includes a gene expression analysis result acquisition unit that acquires the result of analyzing the gene expression in the skin of a subject, a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition, and is provided with the gene related to the specific skin condition is a gene in which the increase or decrease in the expression level was observed in both groups when a plurality of persons were classified into a group having a specific skin condition and a group not having a specific skin condition, It is also a device for estimating the skin condition of a subject.

[0032] In addition, the present invention includes a gene expression analysis result acquisition unit that acquires the result of analyzing the gene expression in the skin of a subject, a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition, a proposal unit that proposes a composition and / or a stimulus (excluding medical treatments) suitable for the subject based on the estimated skin condition, and is provided with The gene related to the specific skin condition is a gene in which the increase or decrease in the expression level is observed in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition. It is also a proposal device for a composition and / or a stimulus (excluding medical practices) suitable for a subject.

[0033] Further, the present invention is a program for estimating the skin condition of a subject, causing a computer to function as a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result obtained from a gene expression analysis device that analyzes the gene expression in the skin of the subject and the expression level of the gene related to the specific skin condition, The gene related to the specific skin condition is also a program in which the increase or decrease in the expression level is observed in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition.

[0034] Further, the present invention is a program for proposing a composition and / or a stimulus (excluding medical practices) suitable for a subject, causing a computer to function as a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result obtained from a gene expression analysis device that analyzes the gene expression in the skin of the subject and the expression level of the gene related to the specific skin condition, and a proposal unit that proposes a composition and / or a stimulus (excluding medical practices) suitable for the subject based on the estimated skin condition, The gene related to the specific skin condition is also a program in which the increase or decrease in the expression level is observed in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition.

[0035] Further, the present invention is also a method for estimating the skin condition of a subject, which performs gene expression analysis using the skin collected from the subject by micro-biopsy and estimates the skin condition of the subject based on the result.

[0036] In addition, the present invention includes a gene expression analysis step of analyzing the gene expression in the skin of a subject, and a mental state estimation step of estimating the mental state of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific mental state. The gene related to the specific mental state is a gene in which an increase or decrease in the expression level is observed in both a group of a plurality of persons having a specific mental state and a group of persons not having a specific mental state, and is also a method for estimating the mental state of a subject.

Effects of the Invention

[0037] According to the present invention, it is possible to provide a novel method for estimating the skin condition of a subject using the skin. Further, based on the estimated skin condition of the subject, it is possible to provide a novel method for proposing a composition and / or a stimulus (excluding medical acts) suitable for the subject.

Brief Description of the Drawings

[0038]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

Figure 13

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Figure 15

Figure 16

Mode for Carrying Out the Invention

[0039] <1> Method for Estimating Skin Condition of Subject The present invention includes a gene expression analysis step of analyzing the expression of genes in the skin of a subject, A skin condition estimation step of estimating the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition; having The gene related to the specific skin condition is a gene in which an increase or decrease in the expression level is observed in both groups when a plurality of people are classified into a group having a specific skin condition and a group not having a specific skin condition. It is a method for estimating the skin condition of a subject.

[0040] (1) Gene expression analysis step The present invention has a gene expression analysis step of analyzing the expression of genes in the skin of a subject.

[0041] The skin includes the epidermis, dermis and / or subcutaneous tissue, and includes skin pieces and skin cells. The skin preferably includes an epidermal region including the stratum corneum and a dermal region located at a depth of 0.20 to 0.50 mm from the outermost layer of the skin. As the epidermis, in addition to the stratum corneum, it can include the granular layer, spinous layer, and basal layer.

[0042] The dermal region includes a region where the depth from the outermost layer (stratum corneum) of the skin is preferably 0.20 mm or more, more preferably 0.22 mm or more, still more preferably 0.25 mm or more, and particularly preferably 0.28 mm or more. Further, the dermal region includes a region where the depth is preferably 0.75 mm or less, more preferably 0.50 mm or less, still more preferably 0.40 mm or less, and particularly preferably 0.38 mm or less. The depth can be preferably 0.20 to 0.50 mm, more preferably 0.25 to 0.45 mm, and still more preferably 0.28 to 0.40 mm. By setting the position of the dermal region to be acquired within the above range, a sample including the epidermal region and the dermal region can be acquired while reducing the burden on the subject at the time of collection.

[0043] The method for collecting the skin is not particularly limited, but it is preferable to adopt a minimally invasive collection method. Specifically, it is preferable to collect the skin using a needle with a diameter of 1 mm or less. The diameter of the needle is preferably 0.75 mm or less, more preferably 0.50 mm or less, and even more preferably 0.40 mm or less. It is particularly preferable to use micro biopsy for collecting the skin.

[0044] The amount of skin collected is not particularly limited, but the volume of the skin immediately after being obtained from the subject is preferably 3 0.05 mm or less, more preferably 3 0.045 mm or less, even more preferably 3 0.04 mm or less, even more preferably 3 0.038 mm or less, 0.02 mm 3 or less, 0.01 mm 3 or less, 0.008 mm 3 or less, 0.005 mm 3 or less, 0.003 mm 3 or less, 0.002 mm 3 or less.

[0045] The site for collecting the skin is not particularly limited. For example, it includes the face, neck, back of the hand, abdomen, foot, fingers, etc. Preferably, the skin is collected from a site where skin diseases are likely to occur or a site that is easy to use for comparison between exposed parts such as the arm and non-exposed parts.

[0046] In the present invention, "gene expression analysis" refers to examining the expression state of genes.

[0047] The method for gene expression analysis is not particularly limited, but it is preferable that comprehensive gene expression analysis is possible. Specifically, mRNA is extracted from the skin, and after appropriate sample processing, comprehensive gene expression analysis (such as RNA-seq) can be performed by transcriptome analysis using a microarray or next-generation sequencer. In addition, histological analysis (such as RNAscope (registered trademark), etc.) can also be used. In addition, as a method for analyzing the expression of each isolated gene, PCR analysis or the like can also be used.

[0048] (2) Skin condition estimation step The present invention includes a skin condition estimation step of estimating the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition.

[0049] The "specific skin condition" in the present invention includes skin inflammation, wrinkles, sagging, enlarged pores, sensitive skin, rough skin, degree of elasticity, degree of skin color, atopic dermatitis, acne, rosacea, red face, and acne. The "specific skin condition" in the present invention is preferably one or more selected from wrinkles, sagging, enlarged pores, sensitive skin, atopic dermatitis, and acne.

[0050] Here, the enlarged pores include the spread of the pore area, the spread of the pore volume, and the spread of the pore depth.

[0051] In addition, the "specific skin condition" in the present invention can be in a form that also includes a skin condition based on information (parameters) related to the water content of the skin, the water evaporation amount of the skin, the sebum amount of the skin, the three-dimensional feeling (volume) of the skin, the height of the cheeks, and the length of the face line.

[0052] The "gene related to a specific skin condition" in the present invention is a gene in which the increase or decrease in the expression level is recognized in both groups when a plurality of people are classified into a group having a specific skin condition and a group not having a specific skin condition.

[0053] The estimation of the skin condition in the present invention includes not only estimating the presence or absence of the skin condition but also estimating the degree of the skin condition.

[0054] As a method for classifying a plurality of individuals into a group having a specific skin condition and a group not having the specific skin condition, there are methods of classifying a plurality of individuals into a group having a specific skin condition and a group not having the specific skin condition based on face images of a plurality of individuals, responses to questionnaires for skin evaluation obtained from a plurality of individuals, diagnosis results by doctors of a plurality of individuals, device analysis results of the skin of a plurality of individuals (such as moisture, TEWL, oil content, and viscoelasticity), and histological discrimination of a plurality of individuals, etc. In the present invention, preferably, a plurality of individuals are classified into a group having a specific skin condition and a group not having the specific skin condition based on face images of a plurality of individuals, responses to questionnaires for skin evaluation obtained from a plurality of individuals, and / or diagnosis results by doctors of a plurality of individuals.

[0055] As a method for classifying a plurality of individuals into a group having a specific skin condition and a group not having the specific skin condition based on face images of a plurality of individuals, the degree of a specific skin condition in a plurality of individuals is graded by an evaluator visually evaluating using the face images, the group with a large degree of the specific skin condition is classified into the group having the specific skin condition, and the group with a small degree of the specific skin condition is classified into the group not having the specific skin condition.

[0056] As a method for classifying a plurality of individuals into a group having a specific skin condition and a group not having the specific skin condition based on responses to questionnaires for skin evaluation obtained from a plurality of individuals, among a plurality of individuals, those who answered that they have a specific skin condition are classified into the group having the specific skin condition, and those who answered that they do not have the specific skin condition are classified into the group not having the specific skin condition.

[0057] As a method for classifying a plurality of individuals into a group having a specific skin condition and a group not having the specific skin condition based on diagnosis results by doctors of a plurality of individuals, among a plurality of individuals, those diagnosed by a doctor as having a specific skin condition are classified into the group having the specific skin condition, and those diagnosed as not having the specific skin condition are classified into the group not having the specific skin condition.

[0058] As a method of classifying a plurality of individuals into a group having a specific skin condition and a group not having a specific skin condition based on the results of instrumental analysis of the skin of the plurality of individuals (such as moisture, TEWL, oil content, and viscoelasticity), a criterion (reference value) is set for information (parameters) related to the specific skin condition, and among the plurality of individuals, those who satisfy the criterion (the reference value) are classified into the group having the specific skin condition, and those who do not satisfy the criterion (the reference value) are classified into the group not having the specific skin condition.

[0059] In the present invention, the "gene related to a specific skin condition" is preferably a gene in which, after classifying a plurality of individuals into a group having a specific skin condition and a group not having a specific skin condition by the above-described method, the gene expression in the skin of a plurality of individuals belonging to both groups is analyzed, and an increase or decrease is observed in the individuals belonging to the group having the specific skin condition as compared with the individuals belonging to the group not having the specific skin condition.

[0060] In the present invention, preferably, the skin condition of the subject is estimated based on the expression level of the gene related to the specific skin condition of the subject and the expression level of the gene related to the specific skin condition of a person not having the specific skin condition. Specifically, when the expression level of the gene related to the specific skin condition of the subject and the expression level of the gene related to the specific skin condition of a person not having the specific skin condition are compared, if a change in the expression level of the gene related to the specific skin condition of the subject is observed, the skin condition of the subject is estimated.

[0061] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from one or more groups selected from the following groups A to F.

[0062] [Group A] [Table 13]

[0063] [Group B]

Table 14

[0064] [Group C]

Table 15

[0065] [Group D]

Table 16

[0066] [Group E]

Table 17

[0067] [Group F]

Table 18

[0068] In addition, the "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from one or more groups selected from the following Groups G to L.

[0069] [Group G]

Table 19

[0070] [Group H]

Table 20

[0071] [Group I]

Table 21

[0072] [Group J]

Table 22

[0073] [Group K]

Table 23

[0074] [Group L]

Table 24

[0075] Gene Ontology is a controlled vocabulary of terms that structures the information held by individual genes and gives them a biological meaning. Each individual term is called a GO Term. Gene Ontology is broadly classified into three categories: Molecular Function, Cellular Component, and Biological Process, each of which has a hierarchical structure.

[0076] When estimating the meaning of variant genes obtained from comprehensive gene analysis, by using Gene Ontology, it is possible to understand which groups of genes related to functions, intracellular locations, and biological reaction processes of organisms are significantly variable.

[0077] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more Gene Ontologies selected from the above Group A.

[0078] As shown in the examples described below, when the "specific skin condition" in the present invention is wrinkles, an increase or decrease in the expression of genes having one or more gene ontologies selected from the above Group A was observed in the people belonging to the group having the specific skin condition as compared with the people belonging to the group not having the specific skin condition. Therefore, it is possible to estimate the presence or absence of wrinkles in the subject based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group A.

[0079] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group A, it is possible to estimate the presence or absence of wrinkles in the subject caused by a change in the expression level of a gene having one or more gene ontologies selected from the above Group A.

[0080] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Extracellular Matrix Organization (GO:0030198, extracellular matrix organization)", "Extracellular Structure Organization (GO:00403062, extracellular structure organization)", and "External Encapsulating Structure Organization (GO:0045229, external encapsulating structure organization)". Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of wrinkles in the subject.

[0081] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, the presence or absence of wrinkles caused by the fluctuation in the expression level of a gene having one or more gene ontologies selected from Extracellular Matrix Organization (GO:0030198), Extracellular Structure Organization (GO:00403062), and External Encapsulating Structure Organization (GO:0045229) in the subject can be estimated.

[0082] Further, the "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above Group G.

[0083] As shown in the examples described later, when the "specific skin condition" in the present invention is wrinkles, compared with a person belonging to a group having no specific skin condition, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above Group A was observed in a person belonging to a group having a specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group G, the presence or absence of wrinkles in the subject can be estimated.

[0084] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group G, the presence or absence of wrinkles caused by the fluctuation in the expression level of a gene having one or more gene ontologies selected from the above Group G in the subject can be estimated.

[0085] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above Group B.

[0086] As shown in the examples described below, when the "specific skin condition" in the present invention is sagging, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above Group B was observed in the group of people having the specific skin condition as compared with the group of people not having the specific skin condition. Therefore, it is possible to estimate the presence or absence of sagging in the subject based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group B.

[0087] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group B, it is possible to estimate the presence or absence of sagging in the subject due to a change in the expression level of a gene having one or more gene ontologies selected from the above Group B.

[0088] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Lymphocyte Chemotaxis (GO:0048247, lymphocyte chemotaxis)", "Chemokine-Mediated Signaling Pathway (GO:0070098, chemokine-mediated signaling pathway)", and "Cellular Response To Chemokine (GO:1990869, cellular response to chemokine)". Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of sagging in the subject.

[0089] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, the presence or absence of sagging in the subject due to the fluctuation in the expression level of one or more genes having gene ontology selected from Lymphocyte Chemotaxis (GO:0048247), Chemokine-Mediated Signaling Pathway (GO:0070098), and Cellular Response To Chemokine (GO:1990869) can be estimated.

[0090] In addition, the "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above H group.

[0091] As shown in the examples described later, when the "specific skin condition" in the present invention is sagging, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above H group was observed in the people belonging to the group having the specific skin condition as compared with the people belonging to the group having no specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of the gene having one or more gene ontologies selected from the above H group, the presence or absence of sagging in the subject can be estimated.

[0092] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the gene having one or more gene ontologies selected from the above H group, the presence or absence of sagging in the subject due to the fluctuation in the expression level of the gene having one or more gene ontologies selected from the above H group can be estimated.

[0093] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above Group C.

[0094] As shown in the examples described later, when the "specific skin condition" in the present invention is enlarged pores, compared with people belonging to the group without the specific skin condition, in people belonging to the group with the specific skin condition, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above Group C was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group C, it is possible to estimate the presence or absence of enlarged pores in the subject.

[0095] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group C, it is possible to estimate the presence or absence of enlarged pores caused by a change in the expression level of a gene having one or more gene ontologies selected from the above Group C in the subject.

[0096] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Regulation Of Sequestering Of Triglyceride (GO:0010889, regulation of sequestration of triglyceride)", "Regulation Of Receptor Signaling Pathway Via STAT (GO:1904892, regulation of receptor signaling pathway via STAT)", and "Positive Regulation Of DNA Binding (GO:0043388, positive regulation of DNA binding)". Based on the gene expression analysis results of the subject and the expression level of the above gene, the presence or absence of enlarged pores in the subject can be estimated.

[0097] In a preferred embodiment of the present invention, based on the gene expression analysis results of the subject and the expression level of the above gene, the presence or absence of enlarged pores caused by the change in the expression level of one or more genes selected from Regulation Of Sequestering Of Triglyceride (GO:0010889), Regulation Of Receptor Signaling Pathway Via STAT (GO:1904892), and Positive Regulation Of DNA Binding (GO:0043388) in the subject can be estimated.

[0098] Also, the "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above Group I.

[0099] As shown in the examples described later, when the "specific skin condition" in the present invention is enlarged pores, compared with the people belonging to the group without the specific skin condition, in the people belonging to the group with the specific skin condition, an increase or decrease in the expression of the gene having one or more gene ontologies selected from the above Group I was observed. Therefore, based on the gene expression analysis results of the subject and the expression level of the gene having one or more gene ontologies selected from the above Group I, the presence or absence of enlarged pores in the subject can be estimated.

[0100] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group I, it is possible to estimate whether or not the subject has enlarged pores due to a change in the expression level of a gene having one or more gene ontologies selected from the above Group I.

[0101] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above Group D.

[0102] As shown in the examples described later, when the "specific skin condition" in the present invention is sensitive skin, compared with a person belonging to a group having no specific skin condition, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above Group D was observed in a person belonging to a group having a specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group D, it is possible to estimate whether or not the subject has sensitive skin.

[0103] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above Group D, it is possible to estimate whether or not the subject has sensitive skin due to a change in the expression level of a gene having one or more gene ontologies selected from the above Group D.

[0104] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Apoptotic Mitochondrial Changes (GO:0008637, mitochondrial changes due to apoptosis)", "Negative Regulation of mRNA Catabolic Process (GO:1902373, negative regulation of the mRNA catabolic process)", and "Mitochondrial Transport (GO:0006839)". Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate whether the subject has sensitive skin.

[0105] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate whether the subject has sensitive skin due to a change in the expression level of a gene having one or more gene ontologies selected from "Apoptotic Mitochondrial Changes (GO:0008637)", "Negative Regulation of mRNA Catabolic Process (GO:1902373)", and "Mitochondrial Transport (GO:0006839)".

[0106] Also, the "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above group J.

[0107] As shown in the examples described later, when the "specific skin condition" in the present invention is sensitive skin, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above group J was observed in the people belonging to the group having the specific skin condition compared to the people belonging to the group not having the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above J group, it is possible to estimate whether the subject has sensitive skin.

[0108] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above J group, it is possible to estimate whether the subject has sensitive skin caused by a change in the expression level of a gene having one or more gene ontologies selected from the above J group.

[0109] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above E group.

[0110] As shown in the examples described below, when the "specific skin condition" in the present invention is atopic dermatitis, compared with a person belonging to a group without the specific skin condition, an increase or decrease in the expression of a gene having one or more gene ontologies selected from the above E group was observed in a person belonging to a group having the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above E group, it is possible to estimate the presence or absence of atopic dermatitis in the subject.

[0111] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above E group, it is possible to estimate the presence or absence of atopic dermatitis caused by the fluctuation of the expression level of a gene having one or more gene ontologies selected from the above E group in the subject.

[0112] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Establishment Of Skin Barrier (GO:0061436, establishment of skin barrier)", "Skin Epidermis Development (GO:0098773, development of epidermis)", and "Triglyceride Metabolic Process (GO:0006641, metabolic process of triglyceride)". Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of atopic dermatitis in the subject.

[0113] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of atopic dermatitis caused by the fluctuation of the expression level of a gene having one or more gene ontologies selected from Establishment Of Skin Barrier (GO:0061436), Skin Epidermis Development (GO:0098773), and Triglyceride Metabolic Process (GO:0006641) in the subject.

[0114] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above K group.

[0115] As shown in the examples described below, when the "specific skin condition" in the present invention is atopic dermatitis, in the group of people with the specific skin condition, compared with the group of people without the specific skin condition, an increase or decrease in the expression of genes having one or more gene ontologies selected from the above K group was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of genes having one or more gene ontologies selected from the above K group, the presence or absence of atopic dermatitis in the subject can be estimated.

[0116] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of genes having one or more gene ontologies selected from the above K group, in the subject, the presence or absence of atopic dermatitis caused by a change in the expression level of genes having one or more gene ontologies selected from the above K group can be estimated.

[0117] The "gene related to a specific skin condition" in the present invention is preferably a gene having one or more gene ontologies selected from the above F group.

[0118] As shown in the examples described below, when the "specific skin condition" in the present invention is acne, in the group of people with the specific skin condition, compared with the group of people without the specific skin condition, an increase or decrease in the expression of genes having one or more gene ontologies selected from the above F group was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of genes having one or more gene ontologies selected from the above F group, the presence or absence of acne in the subject can be estimated.

[0119] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of a gene having one or more gene ontologies selected from the above F group, the presence or absence of acne caused by the change in the expression level of a gene having one or more gene ontologies selected from the above F group in the subject can be estimated.

[0120] The "gene related to a specific skin condition" in the present invention is more preferably a gene having one or more gene ontologies selected from "Inflammatory Response (GO:0006954, inflammatory response)", "Chemokine-Mediated Signaling Pathway (GO:0070098, chemokine-mediated signaling pathway)", and "Cellular Response To Lipopolysaccharide (GO:0071222, cellular response to lipopolysaccharide)". Based on the gene expression analysis result of the subject and the expression level of the above gene, the presence or absence of acne in the subject can be estimated.

[0121] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, the presence or absence of acne caused by the change in the expression level of a gene having one or more gene ontologies selected from Inflammatory Response (GO:0006954), Chemokine-Mediated Signaling Pathway (GO:0070098), and Cellular Response To Lipopolysaccharide (GO:0071222) in the subject can be estimated.

[0122] Also, the "gene related to a specific skin condition" in the present invention is preferably a gene having the gene ontology of the above L group.

[0123] As shown in the examples described below, when the "specific skin condition" in the present invention is acne, in the group of people with the specific skin condition, compared with those belonging to the group without the specific skin condition, an increase or decrease in the expression of genes having the gene ontology of the above L group was observed. Therefore, based on the gene expression analysis results of the subject and the expression level of the gene having the gene ontology of the above L group, the presence or absence of acne in the subject can be estimated.

[0124] In a preferred embodiment of the present invention, based on the gene expression analysis results of the subject and the expression level of the gene having the gene ontology of the above L group, the presence or absence of acne caused by the change in the expression level of the gene having the gene ontology of the above L group in the subject can be estimated.

[0125] In addition, the "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the following groups a to c. [Group a] MMP1, MMP3, CCL21, COL1A1, COL3A1, ELN, CLASP2, COMP, DAG1, FAP, SERPINB5, ELANE, LY6G5B, KRT10, IVL [Group b] MMP1, MMP3, CCL21, CXCR3, TNF, CCR2, RIPK2, AIPL1, ELANE, OGN, COL1A1, COL3A1, LY6G5B [Group c] IGF1, IGFBP3, IL1A, KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, PLIN2 [Group d] FLG, IL4, IL13, KDF1, KRT16, GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, ARTN [Group A] FLG, CCL17, IL4, IL13, CDH1, CLDN1, KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, LORICRIN [Group B] CASP1, CASP4, CD44, CDH5, COL6A1, ELANE, EGFR, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, TRPV1, USP50

[0126] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above Group A.

[0127] As shown in the examples described below, when the "specific skin condition" in the present invention is wrinkles, an increase or decrease in the expression of one or more genes selected from the above Group A was observed in people belonging to the group with the specific skin condition compared to people belonging to the group without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above Group A, the presence or absence of wrinkles in the subject can be estimated.

[0128] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above Group A, the presence or absence of wrinkles caused by a change in the expression level of one or more genes selected from the above Group A in the subject can be estimated.

[0129] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from CLASP2, COMP, DAG1, FAP, SERPINA5, ELANE, LY6G5B, KRT10, IVL. Based on the gene expression analysis result of the subject and the expression level of the above gene, the presence or absence of wrinkles in the subject can be estimated.

[0130] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from CLASP2, COMP, DAG1, FAP, SERPINA5, ELANE, LY6G5B, KRT10, and IVL, it is possible to estimate the presence or absence of wrinkles caused by fluctuations in the expression levels of one or more genes selected from CLASP2, COMP, DAG1, FAP, SERPINA5, ELANE, LY6G5B, KRT10, and IVL in the subject.

[0131] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above Group I.

[0132] As shown in the examples described later, when the "specific skin condition" in the present invention is sagging, an increase or decrease in the expression of one or more genes selected from the above Group I was observed in the people belonging to the group with the specific skin condition compared to those belonging to the group without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above Group I, it is possible to estimate the presence or absence of sagging in the subject.

[0133] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above Group I, it is possible to estimate the presence or absence of sagging caused by fluctuations in the expression levels of one or more genes selected from the above Group I in the subject.

[0134] More preferably, the "gene related to a specific skin condition" in the present invention is one or more genes selected from CCR2, RIPK2, AIPL1, ELANE, OGN, and LY6G5B. Based on the gene expression analysis result of the subject and the expression levels of the above genes, it is possible to estimate the presence or absence of sagging in the subject.

[0135] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from CCR2, RIPK2, AIPL1, ELANE, OGN, and LY6G5B, it is possible to estimate the presence or absence of flaccidity in the subject due to the fluctuation of the expression level of one or more genes selected from CCR2, RIPK2, AIPL1, ELANE, OGN, and LY6G5B.

[0136] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above group.

[0137] As shown in the examples described later, when the "specific skin condition" in the present invention is enlarged pores, an increase or decrease in the expression of one or more genes selected from the above group was observed in the people belonging to the group with the specific skin condition compared to those belonging to the group without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group, it is possible to estimate the presence or absence of enlarged pores in the subject.

[0138] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group, it is possible to estimate the presence or absence of enlarged pores in the subject due to the fluctuation of the expression level of one or more genes selected from the above group.

[0139] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, and PLIN2. Based on the gene expression analysis result of the subject and the expression level of the above genes, it is possible to estimate the presence or absence of enlarged pores in the subject.

[0140] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, and PLIN2, it is possible to estimate the presence or absence of enlarged pores caused by fluctuations in the expression levels of one or more genes selected from KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, and PLIN2 in the subject.

[0141] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above group E.

[0142] As shown in the examples described below, when the "specific skin condition" in the present invention is sensitive skin, compared with people belonging to the group without the specific skin condition, an increase or decrease in the expression of one or more genes selected from the above group E was observed in people belonging to the group with the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above group E, it is possible to estimate whether the subject has sensitive skin.

[0143] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above group E, it is possible to estimate whether the subject has sensitive skin caused by fluctuations in the expression levels of one or more genes selected from the above group E.

[0144] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, and ARTN. Based on the gene expression analysis result of the subject and the expression levels of the above genes, it is possible to estimate whether the subject has sensitive skin.

[0145] In a preferred embodiment of the present invention, based on the gene expression analysis results of the subject and the expression levels of one or more genes selected from GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, and ARTN, it is possible to estimate whether the subject has sensitive skin due to fluctuations in the expression levels of one or more genes selected from GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, and ARTN.

[0146] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above group.

[0147] As shown in the examples described below, when the "specific skin condition" in the present invention is atopic dermatitis, compared with people belonging to the group without the specific skin condition, an increase or decrease in the expression of one or more genes selected from the above group was observed in people belonging to the group with the specific skin condition. Therefore, based on the gene expression analysis results of the subject and the expression levels of one or more genes selected from the above group, it is possible to estimate the presence or absence of atopic dermatitis in the subject.

[0148] In a preferred embodiment of the present invention, based on the gene expression analysis results of the subject and the expression levels of one or more genes selected from the above group, it is possible to estimate the presence or absence of atopic dermatitis in the subject due to fluctuations in the expression levels of one or more genes selected from the above group.

[0149] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, and LORICRIN. Based on the gene expression analysis results of the subject and the expression levels of the above genes, it is possible to estimate the presence or absence of atopic dermatitis in the subject.

[0150] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, and LORICRIN, it is possible to estimate the presence or absence of atopic dermatitis caused by the change in the expression level of one or more genes selected from KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, and LORICRIN in the subject.

[0151] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above group C.

[0152] As shown in the examples described below, when the "specific skin condition" in the present invention is acne, an increase or decrease in the expression of one or more genes selected from the above group C was observed in the people belonging to the group having the specific skin condition as compared with the people belonging to the group having no specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group C, it is possible to estimate the presence or absence of acne in the subject.

[0153] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group C, it is possible to estimate the presence or absence of acne caused by the change in the expression level of one or more genes selected from the above group C in the subject.

[0154] More preferably, the "gene related to a specific skin condition" in the present invention is one or more genes selected from ELANE, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, and USP50. Based on the gene expression analysis result of the subject and the expression level of the above genes, it is possible to estimate the presence or absence of acne in the subject.

[0155] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from ELANE, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, and USP50, the presence or absence of acne caused by the fluctuation of the expression level of one or more genes selected from ELANE, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, and USP50 in the subject can be estimated.

[0156] In addition, the "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the following groups. [Group Ki] SMAD3, TGFB1, MMP2, MMP9, MMP13, FOS, JUN, RFX5, IRF1, STAT1, BMP2, STAT3 [Group Ku] ELN, MMP12, MME, CSF2 [Group Ke] ALOX15, SLC27A1, SREBF1, DGAT1, FASN, ELF4, HMGCR [Group Ko] IL1B, TLR2, CXCL8, CXCL2, LCN2, STAT1, IFNG, IRF1, IL17A, TNF, TRPV1, TRPV2, TRPV3, TRPV4, TRPM2, TRPM4, TRPC6 [Group Sa] MMP12, IL1A, IL1B, IL36G, IFNG, CXCL10, STAT3, TSLP, STAT1, CXCL8, IL4R, CCL18, CCL2, IL17A, IL23A, S100A7, S100A8, S100A9, S100A12, JUN, TNF, FOS [Group Shi] CD14, LY96, TNFSF13B, JUN, EIF2AK2, TREM1

[0157] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above Group Ki.

[0158] As shown in the examples described below, when the "specific skin condition" in the present invention is wrinkles, an increase or decrease in the expression of one or more genes selected from the above-mentioned group was observed in the group of people with the specific skin condition compared to the group of people who do not have the specific skin condition. Therefore, it is possible to estimate the presence or absence of wrinkles in the subject based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group.

[0159] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group, it is possible to estimate the presence or absence of wrinkles caused by a change in the expression level of one or more genes selected from the above-mentioned group in the subject.

[0160] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from RFX5, IRF1, STAT1, BMP2, and STAT3. Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of wrinkles in the subject.

[0161] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from RFX5, IRF1, STAT1, BMP2, and STAT3, it is possible to estimate the presence or absence of wrinkles caused by a change in the expression level of one or more genes selected from RFX5, IRF1, STAT1, BMP2, and STAT3 in the subject.

[0162] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above-mentioned group.

[0163] As shown in the examples described below, when the "specific skin condition" in the present invention is sagging, an increase or decrease in the expression of one or more genes selected from the above-mentioned group K was observed in the group of people with the specific skin condition compared to the group of people without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group K, it is possible to estimate the presence or absence of sagging in the subject.

[0164] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group K, it is possible to estimate the presence or absence of sagging in the subject caused by a change in the expression level of one or more genes selected from the above-mentioned group K.

[0165] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above-mentioned group G.

[0166] As shown in the examples described below, when the "specific skin condition" in the present invention is enlarged pores, an increase or decrease in the expression of one or more genes selected from the above-mentioned group G was observed in the group of people with the specific skin condition compared to the group of people without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group G, it is possible to estimate the presence or absence of enlarged pores in the subject.

[0167] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group G, it is possible to estimate the presence or absence of enlarged pores in the subject caused by a change in the expression level of one or more genes selected from the above-mentioned group G.

[0168] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above-mentioned group C.

[0169] As shown in the examples described below, when the "specific skin condition" in the present invention is sensitive skin, an increase or decrease in the expression of one or more genes selected from the above group was observed in the group of people with the specific skin condition compared to the group of people without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group, it is possible to estimate whether the subject has sensitive skin.

[0170] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above group, it is possible to estimate whether the subject has sensitive skin caused by a change in the expression level of one or more genes selected from the above group.

[0171] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above subgroup.

[0172] As shown in the examples described below, when the "specific skin condition" in the present invention is atopic dermatitis, an increase or decrease in the expression of one or more genes selected from the above subgroup was observed in the group of people with the specific skin condition compared to the group of people without the specific skin condition. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above subgroup, it is possible to estimate the presence or absence of atopic dermatitis in the subject.

[0173] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above subgroup, it is possible to estimate the presence or absence of atopic dermatitis in the subject caused by a change in the expression level of one or more genes selected from the above subgroup.

[0174] The "gene related to a specific skin condition" in the present invention is preferably one or more genes selected from the above-mentioned group of genes.

[0175] As shown in the examples described below, when the "specific skin condition" in the present invention is acne, compared with people belonging to the group without the specific skin condition, in people belonging to the group with the specific skin condition, an increase or decrease in the expression of one or more genes selected from the above-mentioned group of genes was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group of genes, the presence or absence of acne in the subject can be estimated.

[0176] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above-mentioned group of genes, it is possible to estimate the presence or absence of acne in the subject caused by a change in the expression level of one or more genes selected from the above-mentioned group of genes.

[0177] The "gene related to a specific skin condition" in the present invention is more preferably one or more genes selected from LY96, TNFSF13B, JUN, EIF2AK2, and TREM1. Based on the gene expression analysis result of the subject and the expression level of the above genes, the presence or absence of acne in the subject can be estimated.

[0178] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from LY96, TNFSF13B, JUN, EIF2AK2, and TREM1, it is possible to estimate the presence or absence of acne in the subject caused by a change in the expression level of one or more genes selected from LY96, TNFSF13B, JUN, EIF2AK2, and TREM1.

[0179] <2> Method for Proposing a Composition and / or Stimulus (Excluding Medical Acts) Suitable for a Subject The present invention includes a gene expression analysis step for analyzing the gene expression in the skin of a subject, a skin condition estimation step for estimating the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition, a proposal step for proposing a composition and / or a stimulus (excluding medical acts) suitable for the subject based on the estimated skin condition, and has the gene related to the specific skin condition is a gene in which an increase or decrease in the expression level is observed in both a group of a plurality of persons classified into a group having the specific skin condition and a group not having the specific skin condition, It is also a method for proposing a composition and / or a stimulus (excluding medical acts) suitable for a subject.

[0180] The composition and / or the stimulus (excluding medical acts) of the present invention can be applied without particular limitation as long as they are commonly known.

[0181] As described above, the stimulus of the present invention does not include medical acts. Here, the "medical act" in the present invention means an act in which a doctor (including a person who has received instructions from a doctor) performs surgery, treatment, or diagnosis on a human. Specifically, the stimulus of the present invention does not include surgical procedures on the human body.

[0182] Examples of the composition of the present invention include commercially available compounds, known compounds, groups of compounds obtained by combinatorial chemistry techniques, natural ingredients derived from plants and marine organisms, and substances such as animal tissue extracts.

[0183] Extracts derived from animals and plants mean not only the extracts themselves derived from animals or plants, but also the general names of fractions of the extracts, purified fractions, solvent-removed products of the extracts or fractions, and purified products. In addition, examples of the plant-derived extracts include extracts using plants grown naturally or cultivated, extracts using those sold as raw materials for Kampo medicines, and commercially available extracts.

[0184] In addition, examples of the composition of the present invention include cosmetics and external skin preparations containing the above compounds, natural ingredients, and substances.

[0185] Examples of the stimuli of the present invention include face packs, massage techniques, aromatherapy, laser light irradiation, electric pulses, iontophoresis, electroporation, ultrasonic waves, microneedles, and the like.

[0186] <3>Device for estimating the skin condition of a subject The present invention includes a gene expression analysis result acquisition unit that acquires the result of analyzing the gene expression in the skin of a subject, and a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition. It is provided with The gene related to the specific skin condition is a gene in which the increase or decrease in the expression level is recognized in both groups when a plurality of people are classified into a group having a specific skin condition and a group not having a specific skin condition. It is also a device for estimating the skin condition of a subject.

[0187] Hereinafter, an explanation will be given with reference to the hardware block diagram (Figure 1) of the estimation device of the present invention. The estimation device of the present invention is a device for implementing the method for estimating the skin condition of a subject described in the item <1> above. Therefore, the explanation in the item <1> above is also valid for the following estimation device.

[0188] The estimation device 1 of the present invention includes a gene expression analysis result acquisition unit 11 that acquires the result of analyzing the gene expression in the skin of a subject, and a skin condition estimation unit 131 that estimates the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific skin condition.

[0189] As shown in FIG. 1, the estimation device 1 includes a gene expression analysis result acquisition unit 11 that acquires the result of analyzing the gene expression in the skin of a subject, a ROM (Read Only Memory) 12 having a storage unit 121, a CPU (Central Processing Unit) 13 having a skin condition estimation unit 131 that estimates the skin condition of the subject, and an estimation result display unit 14.

[0190] The gene expression analysis result acquisition unit 11 acquires the result of analyzing the gene expression in the skin of the subject. Specifically, it acquires the data of the expression level of the gene related to a specific skin condition calculated as a result of performing the gene expression analysis.

[0191] The storage unit 121 stores the relevance between a specific skin condition and a gene. For example, it stores that the expression variation of genes having GO Terms related to extracellular matrix organization and extracellular structure organization is related to wrinkles. Also, for example, it stores that the expression variation of genes such as MMP1 and SERPINB5 is related to wrinkles.

[0192] The skin condition estimation unit 131 estimates the skin condition of the subject based on the gene expression analysis result acquired by the gene expression analysis result acquisition unit 11 and the gene related to a specific skin condition stored in the storage unit 121.

[0193] The estimation result display unit 14 is a display that displays the result estimated by the skin condition estimation unit 131.

[0194] <4>Proposal device for a composition and / or stimulus suitable for a subject (excluding medical acts) The present invention includes a gene expression analysis unit that analyzes the gene expression in the skin of a subject, a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result of the subject and the expression level of the gene related to a specific skin condition, A proposal unit that proposes a composition and / or a stimulus (excluding medical practices) suitable for the subject based on the presumed skin condition; comprising; The gene related to the specific skin condition is a gene in which the increase or decrease in the expression level is recognized in both groups when a plurality of people are classified into a group having a specific skin condition and a group not having a specific skin condition. It is also a proposal device for a composition and / or a stimulus (excluding medical practices) suitable for the subject.

[0195] Hereinafter, the description will be added with reference to the hardware block diagram (Figure 2) of the proposal device of the present invention. The proposal device of the present invention is a device for implementing the proposal method for a composition and / or a stimulus (excluding medical practices) suitable for the subject described in item <2> above. Therefore, the description in item <2> above is also valid for the following proposal device.

[0196] The proposal device 2 of the present invention includes a gene expression analysis result acquisition unit 21 that acquires the result of analyzing the gene expression in the skin of the subject, and based on the gene expression analysis result of the subject and the expression level of the gene related to the specific skin condition, a skin condition estimation unit 231 that estimates the skin condition of the subject, and a proposal unit 232 that proposes a composition and / or a stimulus (excluding medical practices) suitable for the subject based on the presumed skin condition.

[0197] As shown in Figure 2, the proposal device 2 includes a gene expression analysis result acquisition unit 21 that acquires the result of analyzing the gene expression in the skin of the subject, a ROM (Read Only Memory) 22 including a first storage unit 221 and a second storage unit 222, a CPU (Central Processing Unit) 23 including a skin condition estimation unit 231 that estimates the skin condition of the subject and a proposal unit 232 that proposes a composition and / or a stimulus (excluding medical practices) suitable for the subject based on the presumed skin condition, and a proposal result display unit 24.

[0198] The gene expression analysis result acquisition unit 21 acquires the results of analyzing the gene expression in the skin of the subject. Specifically, it acquires the data of the expression levels of genes related to a specific skin condition, which are calculated as a result of performing gene expression analysis.

[0199] The first storage unit 221 stores the relationship between a specific skin condition and genes. For example, it stores that the expression fluctuations of genes having GO Terms related to extracellular matrix organization or extracellular structure organization are related to wrinkles. Also, for example, it stores that the expression fluctuations of genes such as MMP1 and SERPINB5 are related to wrinkles.

[0200] The skin condition estimation unit 231 estimates the skin condition of the subject based on the gene expression analysis results acquired by the gene expression analysis result acquisition unit 21 and the genes related to a specific skin condition stored in the first storage unit 221.

[0201] The second storage unit 222 stores compositions and / or stimuli (excluding medical acts) that can decrease (or increase) the expression levels of genes related to a specific skin condition.

[0202] The proposal unit 232 proposes a composition and / or stimulus suitable for the subject based on the skin condition of the subject estimated by the skin condition estimation unit 231 and the compositions and / or stimuli (excluding medical acts) stored in the second storage unit 222.

[0203] The proposal result display unit 24 is a display that displays the results proposed by the proposal unit 232.

[0204] <5> Program for estimating the skin condition of a subject The present invention is a program for estimating the skin condition of a subject, causing a computer to Function as a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result obtained from a gene expression analysis device that analyzes the gene expression in the skin of the subject and the expression level of genes related to a specific skin condition. The gene related to the specific skin condition is also a program that classifies a plurality of people into a group having the specific skin condition and a group not having the specific skin condition, and is a gene in which an increase or decrease in the expression level is observed in both groups.

[0205] The estimation program of the present invention corresponds to each means in the CPU included in the estimation device described in item <3> above. Therefore, the description of item <3> above is also valid for the proposed device of the present invention.

[0206] <6> Program for proposing a composition and / or stimulus suitable for a subject (excluding medical acts) Further, the present invention is a program for proposing a composition and / or stimulus (excluding medical acts) suitable for a subject, Cause a computer to Function as a skin condition estimation unit that estimates the skin condition of the subject based on the gene expression analysis result obtained from a gene expression analysis device that analyzes the gene expression in the skin of the subject and the expression level of genes related to a specific skin condition, and Function as a proposal unit that proposes a composition and / or stimulus (excluding medical acts) suitable for the subject based on the estimated skin condition, and The gene related to the specific skin condition is also a program that classifies a plurality of people into a group having the specific skin condition and a group not having the specific skin condition, and is a gene in which an increase or decrease in the expression level is observed in both groups.

[0207] The proposal program of the present invention corresponds to each means in the CPU included in the proposal device described in item <4> above. Therefore, the description of item <4> above is also valid for the proposal device of the present invention.

[0208] <7> Method for estimating the mental state of a subject The present invention includes a gene expression analysis step of analyzing the gene expression in the skin of a subject, and a mental state estimation step of estimating the mental state of the subject based on the gene expression analysis result of the subject and the expression level of a gene related to a specific mental state, and has the gene related to the specific mental state is a gene in which an increase or decrease in the expression level is observed in both groups when a plurality of people are classified into a group having a specific mental state and a group not having a specific mental state. It is a method for estimating the mental state of a subject.

[0209] Here, the description of the method for estimating the mental state of the subject in the present invention can be applied by reading the "skin state" in <1> above as "mental state". Hereinafter, the method for estimating the mental state of the subject in the present invention will be additionally described.

[0210] The "specific mental state" in the present invention is preferably a mental health disorder. The "mental health disorder" in the present invention means "including a wide range of mental and behavioral problems that may affect the physical and mental health, social life and quality of life of workers, such as mental disorders classified as mental and behavioral disorders and suicide, as well as stress, strong worries, anxiety, etc.".

[0211] The "gene related to a specific mental state" in the present invention is preferably a gene having one or more gene ontologies selected from the following M group.

[0212] [M group]

Table 25

[0213] As shown in the examples described below, when the "specific mental state" in the present invention is mental health disorder, in the people belonging to the group having the specific mental state, compared with the people belonging to the group not having the specific mental state, an increase or decrease in the expression of genes having one or more gene ontologies (Gene Ontology) selected from the above M group was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of the gene having one or more gene ontologies (Gene Ontology) selected from the above M group, it is possible to estimate the presence or absence of mental health disorder in the subject.

[0214] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the gene having one or more gene ontologies (Gene Ontology) selected from the above M group, it is possible to estimate the presence or absence of mental health disorder caused by the change in the expression level of the gene having one or more gene ontologies (Gene Ontology) selected from the above M group in the subject.

[0215] The "gene related to a specific mental state" in the present invention is more preferably a gene having one or more gene ontologies (Gene Ontology) selected from "Negative Regulation Of Immune Response (GO:0050777, negative regulation of immune response)", "Negative Regulation Of Immune System Process (GO:0002683, negative regulation of immune system process)", and "Regulation Of T Cell Cytokine Production (GO:0002724, regulation of T cell cytokine production)". Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of mental health disorder in the subject.

[0216] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of the above gene, in the subject, Negative Regulation Of Immune Response (GO:0050777), Negative Regulation Of Immune System Process (GO:0002683), Regulation Of T Cell Cytokine Production (GO:0002724), it is possible to estimate the presence or absence of mental health disorder caused by the change in the expression level of one or more genes having a gene ontology selected therefrom.

[0217] The "gene related to a specific mental state" in the present invention is preferably one or more genes selected from the following gene group. [Gene group] ADAR, ATG5, C4BPA, CCR1, CCR2, CD59, COL3A1, FOXP3, GPX1, IL33, SMAD7, TYRO3, WDR41

[0218] As shown in the examples described later, when the "specific mental state" in the present invention is a mental health disorder, compared with a person belonging to a group having no specific mental state, in a person belonging to a group having a specific mental state, an increase or decrease in the expression of one or more genes selected from the above gene group was observed. Therefore, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above gene group, it is possible to estimate the presence or absence of mental health disorder in the subject.

[0219] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from the above gene group, in the subject, it is possible to estimate the presence or absence of mental health disorder caused by the change in the expression level of one or more genes selected from the above gene group.

[0220] The "gene related to a specific mental state" in the present invention is more preferably one or more genes selected from ADAR, ATG5, C4BPA, CD59, FOXP3, GPX1, IL33, SMAD7, TYRO3, and WDR41. Based on the gene expression analysis result of the subject and the expression level of the above gene, it is possible to estimate the presence or absence of mental health disorder in the subject.

[0221] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression level of one or more genes selected from ADAR, ATG5, C4BPA, CD59, FOXP3, GPX1, IL33, SMAD7, TYRO3, and WDR41, it is possible to estimate the presence or absence of mental health disorder in the subject caused by the fluctuation of the expression level of one or more genes selected from ADAR, ATG5, C4BPA, CD59, FOXP3, GPX1, IL33, SMAD7, TYRO3, and WDR41.

[0222] Also, the "gene related to a specific mental state" in the present invention is preferably a gene having the following N-group gene ontology.

[0223] [N-group]

Table 26

[0224] As shown in the examples described later, when the "specific mental state" in the present invention is a mental health disorder, an increase or decrease in the expression of the gene having the above N-group gene ontology was observed in the people belonging to the group having the specific mental state as compared with the people belonging to the group not having the specific mental state. Therefore, based on the gene expression analysis result of the subject and the expression level of the gene having the above N-group gene ontology, it is possible to estimate the presence or absence of mental health disorder in the subject.

[0225] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of the genes having the above-mentioned N groups of Gene Ontology, it is possible to estimate the presence or absence of mental health disorders caused by fluctuations in the expression levels of the genes having the above-mentioned N groups of Gene Ontology in the subject.

[0226] The "gene related to a specific mental state" in the present invention is preferably one or more genes selected from the following group C. [Group C] JUN, ICAM1, ACKR3, MSR1, PRIM2, PLAU, FYN

[0227] As shown in the examples described later, when the "specific mental state" in the present invention is a mental health disorder, an increase or decrease in the expression of one or more genes selected from the above group C was observed in the people belonging to the group having the specific mental state as compared with the people belonging to the group not having the specific mental state. Therefore, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above group C, it is possible to estimate the presence or absence of mental health disorders in the subject.

[0228] In a preferred embodiment of the present invention, based on the gene expression analysis result of the subject and the expression levels of one or more genes selected from the above group C, it is possible to estimate the presence or absence of mental health disorders caused by fluctuations in the expression levels of one or more genes selected from the above group C in the subject.

Example

[0229] <Test Example 1> Extraction of genes serving as indicators for estimating skin condition (1) In Test Example 1, when the "specific skin condition" is wrinkles, genes serving as indicators for estimating that the skin condition of the subject is wrinkles are extracted.

[0230] First, a plurality of individuals were classified into a group with wrinkles and a group without wrinkles using visual evaluation criteria. The visual evaluation criteria is a table in which the degree of wrinkles and grades (grades 0 to 7 are prepared according to the degree of wrinkles) are associated in advance. Five individuals with a wrinkle degree of grade 2 or less were classified into the group without wrinkles, and five individuals with a wrinkle degree of grade 3 or more were classified into the group with wrinkles.

[0231] Next, the gene expression in the skin of a plurality of individuals included in each group was analyzed. As the skin, skin collected from the wrinkled and non-wrinkled areas on the faces of a plurality of individuals was used. Here, the prepared skin had a collection amount of 0.05 mm 3 or less. In gene expression analysis, RNA was extracted from the prepared skin using the Picopure kit (manufactured by Thermo fisher). Then, using a Bioanalyzer (manufactured by Agilent), the quality of the extracted RNA was confirmed. Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0232] As a result of the gene expression analysis, the genes shown in FIGS. 3 and 4 were found to have increased or decreased expression in the individuals belonging to the group with wrinkles compared to those belonging to the group without wrinkles. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 3 and 4 as an index. In particular, it was found that the skin condition of the subject can be estimated to be wrinkled using the expression of the genes shown in FIGS. 3 and 4 as an index.

[0233] In addition, as a result of performing the above gene expression analysis, a group of genes showing significant increases or decreases in gene expression were extracted from the skin samples taken from the wrinkled areas, and gene ontology (GO) enrichment analysis of biological process (BP) was performed using Enrichr (https: / / maayanlab.cloud / Enrichr / ), a public web tool. The results are shown in Tables 27 and 28.

[0234]

Table 27

[0235]

Table 28

[0236] As a result of the enrichment analysis, it was found that GO Terms related to extracellular matrix organization and extracellular structure organization appeared significantly.

[0237] By comprehensively evaluating the expression changes of genes having one or more gene ontologies selected from Tables 27 and 28 above, the skin condition of the subject can be estimated. In particular, the skin condition of the subject can be estimated to be wrinkled using the expression of genes having one or more gene ontologies selected from Tables 27 and 28 above as an index.

[0238] <Test Example 2> Extraction of genes serving as indicators for estimating skin condition (2) In Test Example 2, when the "specific skin condition" is sagging, genes serving as indicators for estimating that the skin condition of the subject is sagging are extracted.

[0239] First, a plurality of individuals were classified into a group with sagging and a group without sagging according to the method of a previously reported document (https: / / www.jstage.jst.go.jp / article / sccj / 49 / 2 / 49_114 / _pdf / -char / ja). The five individuals with the lowest degree of sagging were classified into the group without sagging, and the five individuals with the highest degree of sagging were classified into the group with sagging.

[0240] Next, the gene expression in the skin of a plurality of individuals included in each group was analyzed. As the skin, skin samples collected from the sagging and non-sagging areas on the faces of a plurality of individuals were used. Here, the amount of the prepared skin samples was 0.05 mm 3 or less. In the gene expression analysis, RNA was extracted from the prepared skin samples using the Picopure kit (manufactured by Thermo Fisher). Then, the quality of the extracted RNA was confirmed using a Bioanalyzer (manufactured by Agilent). Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0241] As a result of the gene expression analysis, the genes shown in FIGS. 5 and 6 were found to have increased or decreased expression in the individuals belonging to the group with sagging compared to those belonging to the group without sagging. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 5 and 6 as an index. In particular, it was found that the skin condition of the subject can be estimated to be sagging using the expression of the genes shown in FIGS. 5 and 6 as an index.

[0242] In addition, as a result of performing the above gene expression analysis, a group of genes showing significant increases or decreases in gene expression were extracted from the skin collected from the sagging site, and biological process (BP) was explored by gene ontology (GO) enrichment analysis using the public web tool Enrichr (https: / / maayanlab.cloud / Enrichr / ). The results are shown in Tables 29 and 30.

[0243]

Table 29

[0244]

Table 30

[0245] As a result of the enrichment analysis, it was found that GO Terms related to lymphocyte chemotaxis and chemokine-mediated signaling pathway appeared significantly.

[0246] By comprehensively evaluating the expression changes of genes having one or more gene ontologies selected from Tables 29 and 30 above, the skin condition of the subject can be estimated. In particular, it is possible to estimate that the skin condition of the subject is sagging using the expression of genes having one or more gene ontologies selected from Tables 29 and 30 above as an index.

[0247] <Test Example 3> Extraction of Genes Serving as Indicators for Estimation of Skin Condition (3) In Test Example 3, when the "specific skin condition" is enlarged pores, genes serving as indicators for estimating that the skin condition of the subject is enlarged pores are extracted.

[0248] First, the number of pores of a plurality of individuals was calculated using pore analysis with VISIA-Evolution (Canfield, VISIA is a registered trademark). Then, five individuals with 80 or fewer calculated pore numbers were classified into a group without enlarged pores, and five individuals with more than 80 calculated pore numbers were classified into a group with enlarged pores.

[0249] Next, the gene expression in the skin of a plurality of individuals included in each group was analyzed. As the skin, skin collected from the enlarged pore sites and non-enlarged pore sites on the faces of a plurality of individuals was used. Here, the prepared skin had a collection amount of 0.05 mm 3 or less. In gene expression analysis, RNA was extracted from the prepared skin using the Picopure kit (manufactured by Thermo fisher). Then, using a Bioanalyzer (manufactured by Agilent), the quality of the extracted RNA was confirmed. Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0250] As a result of gene expression analysis, the genes shown in FIGS. 7 and 8 were found to have increased or decreased expression in the group of people with enlarged pores compared to those in the group of people without enlarged pores. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 7 and 8 as an index. In particular, it was found that the skin condition of the subject can be estimated to be enlarged pores using the expression of the genes shown in FIGS. 7 and 8 as an index.

[0251] In addition, as a result of performing the above gene expression analysis, a gene group with significantly increased or decreased gene expression was extracted from the skin collected from the enlarged pore sites, and a search for biological process (BP) was performed by gene ontology (GO) enrichment analysis using Enrichr (https: / / maayanlab.cloud / Enrichr / ), a public web tool. The results are shown in Tables 31 and 32.

[0252]

Table 31

[0253]

Table 32

[0254] As a result of the enrichment analysis, it was found that GO terms related to the regulation of sequestering of triglyceride and the regulation of receptor signaling pathway via STAT significantly appeared.

[0255] By comprehensively evaluating the expression changes of genes having one or more gene ontologies selected from Table 31 and Table 32 above, the skin condition of the subject can be estimated. In particular, the skin condition of the subject can be estimated to be enlarged pores using the expression of genes having one or more gene ontologies selected from Table 31 and Table 32 above as an index.

[0256] <Test Example 4> Extraction of Genes Serving as Indicators for Estimation of Skin Condition (4) In Test Example 4, when the "specific skin condition" is sensitive skin, genes serving as indicators for estimating that the skin condition of the subject is sensitive skin are extracted.

[0257] First, a questionnaire regarding whether each of a plurality of people has sensitive skin was conducted. Then, five people who answered that they do not have sensitive skin were classified into the group without sensitive skin, and five people who answered that they have sensitive skin were classified into the group with sensitive skin.

[0258] Next, the gene expression in the skin of a plurality of people included in each group was analyzed. As the skin, skin collected from sensitive skin areas and non-sensitive skin areas on the faces of multiple individuals was used. Here, the prepared skin had a collection amount of 0.05 mm 3 or less. In gene expression analysis, RNA was extracted from the prepared skin using the Picopure kit (manufactured by Thermo fisher). Then, using a Bioanalyzer (manufactured by Agilent), the quality of the extracted RNA was confirmed. Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0259] As a result of gene expression analysis, the genes shown in FIGS. 9 and 10 were found to have increased or decreased expression in the group of people with sensitive skin compared to those in the group without sensitive skin. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 9 and 10 as an index. In particular, it was found that the skin condition of the subject can be estimated to be sensitive skin using the expression of the genes shown in FIGS. 9 and 10 as an index.

[0260] In addition, as a result of performing the above gene expression analysis, a gene group with significantly increased or decreased gene expression was extracted from the skin collected from people belonging to the group with sensitive skin, and biological process (BP) was searched by gene ontology (GO) enrichment analysis using the public web tool Enrichr (https: / / maayanlab.cloud / Enrichr / ). The results are shown in Tables 33 and 34.

[0261]

Table 33

[0262]

Table 34

[0263] As a result of the enrichment analysis, it was found that GO Terms related to apoptotic mitochondrial changes and negative regulation of the mRNA catabolic process significantly appeared.

[0264] By comprehensively evaluating the expression changes of genes having one or more gene ontologies selected from Table 33 and Table 34 above, the skin condition of a subject can be estimated. In particular, using the expression of genes having one or more gene ontologies selected from Table 33 and Table 34 above as an index, it can be estimated that the skin condition of the subject is sensitive skin.

[0265] <Test Example 5> Extraction of Genes Serving as Indicators for Estimation of Skin Condition (5) In Test Example 5, when the "specific skin condition" is atopic dermatitis, genes serving as indicators for estimating that the skin condition of a subject is atopic dermatitis are extracted.

[0266] First, a questionnaire regarding whether or not they have atopic dermatitis was conducted for a plurality of people. Also, a doctor diagnosed whether or not a plurality of people have atopic dermatitis. Then, people who answered that they do not have atopic dermatitis and / or people who were diagnosed as not having atopic dermatitis by a doctor's diagnosis were classified into a group without atopic dermatitis, and people who answered that they have atopic dermatitis and / or people who were diagnosed as having atopic dermatitis by a doctor's diagnosis were classified into a group with atopic dermatitis.

[0267] Next, the gene expression in the skin of a plurality of people included in each group was analyzed. As the skin, skin collected from atopic dermatitis sites and non-atopic dermatitis sites on the faces of a plurality of people was used. Here, the prepared skin had a sampling amount of 0.05 mm3 It was as follows. In gene expression analysis, RNA was extracted from the prepared skin using the Picopure kit (manufactured by Thermo Fisher). Then, the quality of the extracted RNA was confirmed using a Bioanalyzer (manufactured by Agilent). Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0268] As a result of gene expression analysis, an increase or decrease in expression was observed in the genes shown in FIGS. 11 and 12 in the group of people with atopic dermatitis compared to the group of people without atopic dermatitis. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 11 and 12 as an index. In particular, it was found that the skin condition of the subject can be estimated to be atopic dermatitis using the expression of the genes shown in FIGS. 11 and 12 as an index.

[0269] In addition, as a result of performing the above gene expression analysis, a group of genes showing a significant increase or decrease in gene expression was extracted from the skin collected from people belonging to the group with atopic dermatitis, and biological process (BP) was searched by gene ontology (GO) enrichment analysis using a public web tool, Enrichr (https: / / maayanlab.cloud / Enrichr / ). The results are shown in Tables 35 and 36.

[0270] [Table 35]

[0271] [Table 36]

[0272] As a result of the enrichment analysis, it was found that GO terms related to the establishment of the skin barrier and the development of the skin epidermis appeared significantly.

[0273] By comprehensively evaluating the changes in the expression of genes having one or more gene ontologies selected from Table 35 and Table 36 above, the skin condition of the subject can be estimated. In particular, it is possible to estimate that the skin condition of the subject is atopic dermatitis using the expression of genes having one or more gene ontologies selected from Table 35 and Table 36 above as an index.

[0274] <Test Example 6> Extraction of genes serving as indices for estimating skin condition (6) In Test Example 6, when the "specific skin condition" is acne, genes serving as indices for estimating that the skin condition of the subject is acne are extracted.

[0275] First, VISIA-Evolution (Canfield, VISIA is a registered trademark) was used to evaluate the presence or absence of acne in a plurality of people. Also, a doctor diagnosed whether a plurality of people had acne or not. Then, people diagnosed as not having acne by the doctor's diagnosis were classified into a group without acne, and people diagnosed as having acne by the doctor's diagnosis were classified into a group with acne.

[0276] Next, the gene expression in the skin of a plurality of people included in each group was analyzed. As the skin, skin collected from acne sites and non-acne sites on the faces of a plurality of people was used. Here, the prepared skin had a sampling amount of 0.05 mm 3 or less. In the gene expression analysis, RNA was extracted from the prepared skin using a Picopure kit (manufactured by Thermo fisher). Then, the quality of the extracted RNA was confirmed using a Bioanalyzer (manufactured by Agilent). Subsequently, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0277] As a result of gene expression analysis, it was found that the genes shown in FIGS. 13 and 14 had increased or decreased expression in the group of people with acne compared to those in the group without acne. Therefore, it was found that the skin condition of the subject can be estimated using the expression of the genes shown in FIGS. 13 and 14 as an index. In particular, it was found that the skin condition of the subject can be estimated to be acne using the expression of the genes shown in FIGS. 13 and 14 as an index.

[0278] In addition, as a result of the above gene expression analysis, a group of genes with significantly increased or decreased gene expression was extracted from the skin collected from people belonging to the group with acne, and biological process (BP) was searched by gene ontology (GO) enrichment analysis using Enrichr (https: / / maayanlab.cloud / Enrichr / ), a public web tool. The results are shown in Tables 37 and 38.

[0279]

Table 37

[0280]

Table 38

[0281] As a result of the enrichment analysis, it was found that GO Terms related to inflammatory response and chemokine-mediated signaling pathway appeared significantly.

[0282] By comprehensively evaluating the changes in the expression of genes having one or more Gene Ontologies selected from Table 37 and Table 38 above, the skin condition of a subject can be estimated. In particular, based on the expression of genes having one or more Gene Ontologies selected from Table 37 and Table 38 above as an index, it can be estimated that the skin condition of the subject is acne.

[0283] <Test Example 7> Extraction of Genes Serving as Indicators for Estimating Skin Condition (7) In Test Example 7, when the "specific mental state" is a mental health disorder, genes serving as indicators for estimating that the mental state of the subject is a mental health disorder are extracted.

[0284] First, a questionnaire about the "state of mind" was conducted for a plurality of people. Then, based on the results of the questionnaire, five people without a tendency for mental health disorders were classified into the group without mental health disorders (mentally healthy), and five people with a tendency for mental health disorders were classified into the group with mental health disorders.

[0285] Next, the gene expression in the skin of a plurality of people included in each group was analyzed. Here, the prepared skin had a sampling amount of 0.05 mm 3 or less. In gene expression analysis, RNA was extracted from the prepared skin using the Picopure kit (manufactured by Thermo fisher). Then, using a Bioanalyzer (manufactured by Agilent), the quality of the extracted RNA was confirmed. Thereafter, those with a DV200 value of 60% or more were subjected to RNA-seq according to the protocol of the SMART-seq Stranded kit (manufactured by Takara Bio Inc.).

[0286] As a result of gene expression analysis, the genes shown in FIGS. 15 and 16 showed an increase or decrease in expression in the people belonging to the group with mental health disorders compared to the people belonging to the group without mental health disorders (mentally healthy). Therefore, it was found that the mental state of a subject can be estimated using the gene expression shown in FIGS. 15 and 16 as an index. In particular, it was found that the mental state of a subject can be estimated to be in a mental health disorder using the gene expression shown in FIGS. 15 and 16 as an index.

[0287] In addition, as a result of performing the above gene expression analysis, a gene group in which a significant increase or decrease in gene expression was observed in the skin collected from a person belonging to the group having a mental health disorder was extracted, and gene ontology (GO) enrichment analysis of biological process (BP) was performed using Enrichr (https: / / maayanlab.cloud / Enrichr / ), a public web tool. The results are shown in Tables 39 and 40.

[0288]

Table 39

[0289]

Table 40

[0290] As a result of the enrichment analysis, it was found that GO Terms related to negative regulation of immune response and negative regulation of immune system process appeared significantly.

[0291] By comprehensively evaluating the expression changes of genes having one or more gene ontologies selected from Tables 39 and 40 above, the mental state of a subject can be estimated. In particular, the mental state of a subject can be estimated to be in a mental health disorder using the expression of genes having one or more gene ontologies selected from Tables 39 and 40 above as an index.

Industrial Applicability

[0292] According to the present invention, the skin condition of a subject can be estimated. Further, according to the present invention, a composition and / or a stimulus (excluding medical acts) suitable for the subject can be proposed.

Explanation of Signs

[0293] 1 Estimation device 11 Gene expression analysis result acquisition unit 12 ROM (Read Only Memory) 121 Storage unit 13 CPU (Central Processing Unit) 131 Skin condition estimation unit 14 Estimation result display unit 2 Proposal device 21 Gene expression analysis result acquisition unit 22 ROM 221 First storage unit 222 Second storage unit 23 CPU 231 Skin condition estimation unit 232 Proposal unit 24 Proposal result display unit

Claims

1. A gene expression analysis step of analyzing gene expression in the skin of a subject; a skin condition estimation step of estimating a skin condition of the subject based on a gene expression analysis result of the subject and an expression level of a gene associated with a specific skin condition; having The gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition. A method for estimating a subject's skin condition.

2. The method of claim 1, wherein the gene associated with the specific skin condition is a gene having one or more gene ontologies selected from one or more groups selected from the following groups A to F: [Group A] 【Table 1】 [Group B] 【Table 2】 [Group C] 【Table 3】 [Group D] 【Table 4】 [Group E] 【Table 5】 [Group F] 【Table 6】

3. The method of claim 1, wherein the gene associated with the specific skin condition is a gene having one or more gene ontologies selected from one or more groups selected from the following groups G to L: [G group] 【Table 7】 [Group H] 【Table 8】 [Group I] 【Table 9】 [Group J] 【Table 10】 [Group K] 【Table 11】 [L group] 【Table 12】

4. The method according to claim 1, wherein the gene associated with the specific skin condition is one or more genes selected from the following groups A to F: [Group A] MMP1, MMP3, CCL21, COL1A1, COL3A1, ELN, CLASP2, COMP, DAG1, FAP, SERPINB5, ELANE, LY6G5B, KRT10, IVL [Group I] MMP1, MMP3, CCL21, CXCR3, TNF, CCR2, RIPK2, AIPL1, ELANE, OGN, COL1A1, COL3A1, LY6G5B [Group U] IGF1, IGFBP3, IL1A, KRT16, MKI67, CRH, PPARG, PTGS2, EGF, TRPV1, PLIN1, PLIN2 [Group E] FLG, IL4, IL13, KDF1, KRT16, GBA1, IL18, STMN1, IRAK1, CARD10, CTCF, GSK3A, NGF, SEMA3A, ARTN [Group O] FLG, CCL17, IL4, IL13, CDH1, CLDN1, KDF1, KRT16, GBA1, IL18, STMN1, DSP, PKP1, CTNNA1, LORICRIN [Fox group] CASP1, CASP4, CD44, CDH5, COL6A1, ELANE, EGFR, HYAL2, KRT1, MYO5A, NPY, SIRT2, TNC, TRPV1, USP50

5. The method according to claim 1, wherein the gene associated with the specific skin condition is one or more genes selected from the following groups: [Ki group] SMAD3, TGFB1, MMP2, MMP9, MMP13, FOS, JUN, RFX5, IRF1, STAT1, BMP2, STAT3 [K group] ELN, MMP12, MME, CSF2 [Group] ALOX15, SLC27A1, SREBF1, DGAT1, FASN, ELF4, HMGCR [Group C] IL1B, TLR2, CXCL8, CXCL2, LCN2, STAT1, IFNG, IRF1, IL17A, TNF, TRPV1, TRPV2, TRPV3, TRPV4, TRPM2, TRPM4, TRPC6 [Sa group] MMP12, IL1A, IL1B, IL36G, IFNG, CXCL10, STAT3, TSLP, STAT1, CXCL8, IL4R, CCL18, CCL2, IL17A, IL23A, S100A7, S100A8, S100A9, S100A12, JUN, TNF, FOS [Shi group] CD14, LY96, TNFSF13B, JUN, EIF2AK2, TREM1

6. The method of any one of claims 1 to 5, wherein the gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups by classifying a plurality of individuals into a group having the specific skin condition and a group not having the specific skin condition based on facial images of the plurality of individuals, responses to a skin evaluation questionnaire obtained from the plurality of individuals, and / or diagnostic results of the plurality of individuals by a doctor.

7. The method according to any one of claims 2 to 5, wherein the skin condition is one or more selected from wrinkles, sagging, enlarged pores, sensitive skin, atopic dermatitis, rosacea, red face, and acne.

8. The estimation step estimates the skin condition as follows: Wrinkles caused by changes in expression of genes having one or more gene ontologies selected from group A, Sagging caused by fluctuations in expression of genes having one or more gene ontologies selected from group B; Enlarged pores caused by changes in expression of genes having one or more gene ontologies selected from group C; Sensitive skin caused by changes in expression of genes having one or more gene ontologies selected from group D; Atopic dermatitis caused by a change in expression of a gene having one or more gene ontologies selected from group E. and / or acne caused by alteration of expression of a gene having one or more gene ontologies selected from group F; The method of claim 6, further comprising estimating that

9. The estimation step estimates the skin condition as follows: Wrinkles caused by changes in expression of genes having one or more gene ontologies selected from group G; Sagging caused by fluctuations in expression of genes having one or more gene ontologies selected from group H; Enlarged pores caused by changes in expression of genes having one or more gene ontologies selected from group I; Sensitive skin caused by changes in expression of genes having one or more gene ontologies selected from group J; atopic dermatitis caused by a change in expression of a gene having one or more gene ontologies selected from group K; and / or acne caused by alteration of expression of a gene having one or more gene ontologies selected from group L; The method of claim 6, further comprising estimating that

10. The estimation step estimates the skin condition as follows: Wrinkles caused by changes in the expression of one or more genes selected from group A, Sagging caused by fluctuations in expression of one or more genes selected from group A; Enlarged pores caused by changes in expression of one or more genes selected from group C; Sensitive skin caused by changes in expression of one or more genes selected from group E. Atopic dermatitis caused by the change in expression of one or more genes selected from group E. and / or acne caused by alterations in the expression of genes having one or more gene ontologies selected from the above group; The method of claim 6, further comprising estimating that

11. The estimation step estimates the skin condition as follows: Wrinkles caused by changes in the expression of one or more genes selected from the group G; Sagging caused by fluctuations in expression of one or more genes selected from the group K, Enlarged pores caused by changes in expression of one or more genes selected from the group I, Sensitive skin caused by changes in expression of one or more genes selected from the group C; Atopic dermatitis caused by alteration of expression of one or more genes selected from the group consisting of and / or acne caused by alteration of expression of a gene having one or more gene ontologies selected from the group C; The method of claim 6, further comprising estimating that

12. The method according to any one of claims 1 to 5, wherein the gene expression analysis is performed by PCR or transcriptome analysis using RNA extracted from the skin as a sample.

13. The volume of skin immediately after acquisition from the subject was 0.05 mm ３ The estimation method according to any one of claims 1 to 5, wherein:

14. A gene expression analysis step of analyzing gene expression in the skin of a subject; a skin condition estimation step of estimating a skin condition of the subject based on a gene expression analysis result of the subject and an expression level of a gene associated with a specific skin condition; A suggestion step of suggesting a composition and / or stimulation (excluding medical treatment) suitable for the subject based on the estimated skin condition; having The gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition. A method for suggesting compositions and / or stimuli (excluding medical procedures) suitable for a subject.

15. a gene expression analysis result acquisition unit that acquires a result of analyzing gene expression in the skin of a subject; a skin condition estimation unit that estimates a skin condition of the subject based on a gene expression analysis result of the subject and an expression amount of a gene related to a specific skin condition; Equipped with The gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition. A device for estimating a subject's skin condition.

16. a gene expression analysis result acquisition unit that acquires a result of analyzing gene expression in the skin of a subject; a skin condition estimation unit that estimates a skin condition of the subject based on a gene expression analysis result of the subject and an expression amount of a gene related to a specific skin condition; A suggestion unit that suggests a composition and / or stimulation (excluding medical treatment) suitable for the subject based on the estimated skin condition; Equipped with The gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups when a plurality of individuals are classified into a group having the specific skin condition and a group not having the specific skin condition. A device for suggesting compositions and / or stimuli (excluding medical procedures) appropriate for a subject.

17. A program for estimating a skin condition of a subject, Computer, a skin condition estimation unit that estimates a skin condition of a subject based on a gene expression analysis result obtained from a gene expression analysis device that analyzes gene expression in the skin of the subject and an expression amount of a gene related to a specific skin condition; The program classifies a plurality of individuals into a group having the specific skin condition and a group not having the specific skin condition, and the gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups.

18. A program for suggesting compositions and / or stimuli (excluding medical procedures) suitable for a subject, Computer, a skin condition estimation unit that estimates a skin condition of a subject based on a gene expression analysis result obtained from a gene expression analysis device that analyzes gene expression in the skin of the subject and an expression amount of a gene associated with a specific skin condition; a suggestion unit that suggests a composition and / or stimulation (excluding medical treatment) suitable for the subject based on the estimated skin condition; The program classifies a plurality of individuals into a group having the specific skin condition and a group not having the specific skin condition, and the gene associated with the specific skin condition is a gene whose expression level is found to be increased or decreased in both groups.

19. A method for estimating a skin condition of a subject, comprising: performing gene expression analysis using skin sampled by microbiopsy from the subject; and estimating the skin condition of the subject based on the results of the analysis.

20. A gene expression analysis step of analyzing gene expression in the skin of a subject; a psychological state estimation step of estimating the psychological state of the subject based on the gene expression analysis result of the subject and the expression amount of a gene related to a specific psychological state; having The gene associated with the specific psychological state is a gene whose expression level is increased or decreased in both groups when a plurality of individuals are classified into a group having the specific psychological state and a group not having the specific psychological state. A method for estimating a subject's psychological state.

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