Leptin production enhancer and cosmetic

Abstract

To provide a technology for enhancing the leptin production effect caused by red light.SOLUTION: A technology is provided for enhancing the leptin production effect caused by red light using a leptin production enhancer containing the extract of Centaurea cyanus for enhancing leptin production under irradiation with light of 620 nm to 770 nm. Also provided is a technology for enhancing the leptin production effect caused by red light using a cosmetic containing silica particles having an average particle size of 10 nm to 100 nm, a predetermined amount of an ultraviolet scattering agent, an oily component containing a predetermined amount of an ultraviolet absorbing agent, and water, the total powder content being a predetermined amount.SELECTED DRAWING: Figure 2

Description

Technical Field

[0001] The present invention relates to a technique for enhancing the production of leptin under red light irradiation.

Background Art

[0002] It is already widely known that ultraviolet rays contained in sunlight and the like have adverse effects on the skin such as skin aging, and numerous countermeasures for protecting the skin from ultraviolet rays have been proposed.

[0003] On the other hand, it has been reported that when visible light including red light is visually irradiated, the leptin concentration in the blood increases (Non-Patent Document 1). Leptin is known as an appetite-suppressing hormone, and it has been reported that its secretion increases during sleep, while decreasing both during sleep and upon waking up when sleep is insufficient (Non-Patent Document 2). Although leptin is well-known to be produced from adipocytes, it is also produced from fibroblasts, and it has been reported that autocrine enhances the production and proliferation of type I collagen in fibroblasts (Non-Patent Document 3). That is, it is expected that if the leptin present in the dermis can be increased, the firmness and elasticity of the skin can be improved.

[0004] On the other hand, the extract of Bidens pilosa (also known as Bidens biternata) used in the first aspect of the present invention is known to activate lymphatic vessels and improve swelling and wrinkles due to photoaging by promoting lymphatic lumen formation and the proliferation of lymphatic endothelial cells (Patent Document 1).

[0005] In addition, it is known that irradiation of the skin with red light promotes the recovery of the skin barrier function, and irradiation of the skin with blue light suppresses the recovery of the skin barrier function. Based on this finding, a technique has been developed in which pigment Y2O2S:Eu is used as a first material that absorbs ultraviolet light and emits red light, and fine particle zinc oxide is used as a second material that Rayleigh scatters blue light, thereby increasing the ratio of red light to blue light irradiated on the skin and promoting the recovery of the skin barrier function (Patent Document 2).

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

Patent Document 2

Non - Patent Documents

[0007]

Non - Patent Document 1

Non - Patent Document 2

Non - Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0008] However, it is not known that red light and gentian extract synergistically enhance the production of leptin. Also, it is not known that a specific formulation of a cosmetic containing silica fine particles transmits red light while Rayleigh - scattering blue light. Therefore, an object of the present invention is to provide a technique for enhancing the action of red light on leptin production.

Means for Solving the Problems

[0009] As a result of intensive research to solve the above problems, the present inventors have found that red light has an effect of enhancing the production of leptin in fibroblasts, and that yarrow extract has a synergistic effect of enhancing leptin production by red light. Further, the present inventors have found that a cosmetic having a specific formulation containing silica fine particles can achieve both the scattering of blue light and the transmission of red light, thereby completing the present invention.

[0010] That is, the present invention is as follows. [1] A leptin production enhancer containing yarrow extract for enhancing the production of leptin under light irradiation of 620 nm to 770 nm. [2] The leptin production enhancer according to [1], which enhances the production of leptin in fibroblasts. [3] The leptin production enhancer according to [1] or [2], which is an external preparation for skin. [4] A cosmetic containing silica particles having an average particle diameter of 10 nm to 100 nm, an ultraviolet scattering agent, an oily component containing an ultraviolet absorber, and water, wherein the total content of the powder in the cosmetic is 8.50% by mass or less, the content of the ultraviolet scattering agent in the cosmetic is 0.50% by mass or less, and the content of the ultraviolet absorber in the cosmetic is 8.00% by mass or more. [5] The cosmetic according to [4], wherein at least a part of the surface of the silica particles is hydrophobized. [6] The cosmetic according to [4] or [5], wherein the content of the silica particles in the cosmetic is 0.50% by mass to 8.00% by mass. [7] The cosmetic according to any one of [4] to [6], further containing yarrow extract. [8] The cosmetic according to any one of [4] to [7], which is for enhancing leptin production. [9] The cosmetic according to any one of [4] to [8], which is for enhancing collagen production.

[10] The cosmetic according to any one of [4] to [9], which is for preventing or improving wrinkles and / or sagging. [Advantages of the Invention]

[0011] The present invention provides a technique for enhancing the action of promoting leptin production by red light.

Brief Description of the Drawings

[0012]

Figure 1

Figure 2

Modes for Carrying Out the Invention

[0013] Hereinafter, the present invention will be described in detail. However, the aspects of each item are not limited to the following description, and the present invention also includes cases where a plurality of aspects are arbitrarily combined.

[0014] In each aspect of the present invention, in order to solve the above - mentioned problems, the following configurations are respectively adopted. The first aspect is a leptin production enhancer containing yagurumasou extract for enhancing the production of leptin under light irradiation of 620 nm to 770 nm. The second aspect is a cosmetic containing silica particles with an average particle diameter of 10 nm to 100 nm, an ultraviolet - ray scattering agent, an oily component containing an ultraviolet - ray absorber, and water, wherein the total content of powder in the cosmetic is 8.50 mass% or less, the content of the ultraviolet - ray scattering agent in the cosmetic is 0.50 mass% or less, and the content of the ultraviolet - ray absorber in the cosmetic is 8.00 mass% or more.

[0015] <The first aspect> Hereinafter, each aspect of the present invention will be described. The first aspect is a leptin production enhancer containing a bachelor's button extract (also referred to as "the leptin production enhancer of the present invention") for enhancing the production of leptin under light irradiation of 620 nm to 770 nm.

[0016] The bachelor's button extract is an extract of Centaurea cyanus (scientific name: Centaurea cyanus) of the genus Centaurea in the family Asteraceae. Other names for bachelor's button include Centaurea cyanus, Centaurea, and Centaurea. The origin of bachelor's button is Europe, but it also grows wild or is cultivated in Japan and is easily available from these regions. The method of obtaining bachelor's button is not particularly limited and can be appropriately selected according to the purpose. It may be collected from nature or a commercially available product may be used. The constituent parts of bachelor's button that can be used as extraction raw materials include, for example, above-ground parts such as flower parts, bud parts, fruit parts, pericarp parts, seed parts, seed coat parts, stem parts, leaf parts, branch parts, and branch and leaf parts; and underground parts such as root parts and rhizome parts. These may be used alone or in combination of two or more. Among these, the above-ground part is preferred as the use part of bachelor's button, and the flower part is more preferred.

[0017] The above extract means not only the extract itself, but also the fractions of the extract, purified fractions, and the general term for the solvent-removed products of the extract or fractions and purified products. In addition, as the extract, any extract that is usually used in skin external preparations or oral intake compositions such as cosmetics and pharmaceuticals may be used, and an extract extracted from a plant body by a conventional method can be used.

[0018] As the extraction solvent, one or more selected from polar solvents such as water, alcohols such as ethanol, isopropyl alcohol, and butanol, polyhydric alcohols such as 1,3-butanediol and polypropylene glycol, ketones such as acetone and methyl ethyl ketone, and ethers such as diethyl ether and tetrahydrofuran are preferably used.

[0019] As a specific extraction method, for example, 1 to 30 parts by mass of a solvent is added to 1 part by mass of a part used for extraction of a plant body or the like or its dried substance, and it is immersed for several days at room temperature or for several hours at a temperature near the boiling point, and after cooling to room temperature, if desired, insoluble matters and / or the solvent are removed, and fractionation and purification are performed by column chromatography or the like.

[0020] The extract of *Geranium thunbergii* has an action of synergistically enhancing leptin production by red light. That is, the extract of *Geranium thunbergii* is an active ingredient for enhancing leptin production under red light irradiation.

[0021] In addition, it has been reported that leptin enhances the production and proliferation of type I collagen in fibroblasts (Li P et al., Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 2005 Jan;19(1):20-22.). Therefore, the leptin production enhancer of the present invention can be used as a collagen production enhancer, and by enhancing the production of collagen, it can also be used as a preventive or ameliorating agent for wrinkles and / or sagging.

[0022] Red light is light having a wavelength of 620 nm to 770 nm, preferably 620 nm to 660 nm. The light to be irradiated only needs to contain red light. That is, when the light is spectroscopically analyzed, it only needs to contain the wavelength of light that appears red to the naked eye in its spectrum. Therefore, for example, a form of irradiating sunlight also corresponds to "irradiation with red light". In the present invention, as long as the above red light is applied, there is no limitation on the presence or absence of light of other wavelengths. The light to be irradiated may be artificial light by any light projection means or sunlight. In the case of artificial light, there is no particular limitation on the type of light source, and for example, it may be a light-emitting diode (LED), a halogen lamp, an HID lamp, an incandescent bulb, a silica lamp, or a mini krypton lamp. Also, in the case of artificial light, it may be light emitted from a red LED. For example, light emitted from a red LED having a peak wavelength of 625 nm can be applied. ​

[0023] Red light is not particularly limited. For example, it may be irradiated at an intensity of 0.30 J / cm 2 ~55 W / m 2 under the condition of 2 ~1.50 J / cm 2 and may also be irradiated at an intensity of 0.50 J / cm 2 ~1.20 J / cm 2 and may also be irradiated at an intensity of 0.70 J / cm 2 ~1.00 J / cm 2 and may also be irradiated at an intensity of

[0024] Red light may be irradiated after administering the leptin production enhancer of the present invention, may be irradiated before administering the leptin production enhancer, or may be irradiated simultaneously with the administration of the leptin production enhancer.

[0025] The leptin production enhancing effect can be confirmed by the fact that the amount of leptin produced in cells irradiated with red light and added with the leptin production enhancer is larger than the amount produced in cells irradiated with red light and not added with the leptin production enhancer, or cells not irradiated with red light and added with the leptin production enhancer, usually 110% or more, preferably 120% or more, more preferably 130% or more. The amount of leptin produced can be measured by a known method such as the ELISA method.

[0026] The cells in which the production of leptin is enhanced by the leptin production enhancer of the present invention are not particularly limited as long as they are cells that originally produce leptin, but fibroblasts are preferred.

[0027] The administration route of the leptin production enhancer of the present invention is not particularly limited, such as transdermal, oral, nasal, intravenous injection, etc., but transdermal administration is preferred. Here, "administration" may be replaced with "ingestion". Although the dosage of the leptin production enhancer of the present invention is not particularly limited, considering the desired effects and safety, it is preferably ingested once or in several divided doses as a total amount of the extract of Saururus chinensis of 0.3 to 300 μg / day in terms of solid content. In addition to single-dose ingestion, it is preferably ingested continuously or intermittently for several weeks to several months.

[0028] The leptin production enhancer of the present invention is preferably a topical skin preparation, and preferably includes cosmetics (including quasi-drugs), pharmaceuticals, etc., and more preferably cosmetics. More preferably, there are skin care cosmetics, sunscreen cosmetics, and makeup cosmetics. Examples of skin care cosmetics preferably include lotions, essences, beauty liquids, etc. Sunscreen cosmetics can be for the face, body, etc. Examples of makeup cosmetics preferably include makeup bases, foundations, concealers, BB creams, CC creams, mascaras, eye colors, eyeliners, blushes, eyebrow colors, etc.

[0029] Examples of the dosage form of the topical skin preparation include, but are not particularly limited to, lotion dosage form, emulsion dosage forms such as emulsions and creams, oil dosage form, gel dosage form, powder dosage form, etc.

[0030] The content of the extract of Saururus chinensis in the topical skin preparation of the present invention is preferably 0.00001 to 0.09% by mass, more preferably 0.00005 to 0.03% by mass, and even more preferably 0.0001 to 0.003% by mass in terms of solid content based on the entire topical skin preparation. By setting the content within the above range, it is easy to obtain the desired effects and the degree of freedom in formulation design can be ensured. The above content can be appropriately adjusted according to the above-mentioned administration route and the form of the topical skin preparation to be contained.

[0031] In the production of the topical skin preparation of the present invention, components usually used in the formulation of cosmetics, quasi-drugs, pharmaceuticals, etc. can be arbitrarily blended. Examples of such optional components include hydrocarbons such as squalane, petrolatum, and microcrystalline wax; esters such as jojoba oil, carnauba wax, and octyldodecyl oleate; triglycerides such as olive oil, beef tallow, and coconut oil; fatty acids such as stearic acid, oleic acid, and retinoic acid; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; anionic surfactants such as sulfosuccinate esters and sodium polyoxyethylene alkyl sulfate; amphoteric surfactants such as alkyl betaine salts; cationic surfactants such as dialkyl ammonium salts; nonionic surfactants such as sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylene adducts thereof, polyoxyethylene alkyl ethers, and polyoxyethylene fatty acid esters; polyhydric alcohols such as polyethylene glycol, glycerin, and 1,3 - butylene glycol; thickening / gelling agents, antioxidants, ultraviolet absorbers, colorants, preservatives, powders, etc. can be arbitrarily blended.

[0032] <Second Aspect> A second aspect is a cosmetic containing silica particles with an average particle diameter of 10 nm to 100 nm, an ultraviolet scattering agent, an oily component containing an ultraviolet absorber, and water, wherein the total content of powder in the cosmetic is 8.50 mass% or less, the content of the ultraviolet scattering agent in the cosmetic is 0.50 mass% or less, and the content of the ultraviolet absorber in the cosmetic is 8.00 mass% or more (also referred to as "the cosmetic of the present invention"). According to the cosmetic of the present invention, since the cosmetic film does not become white and opaque on the skin and the transparency of the cosmetic film can be maintained, red light can be transmitted. At the same time, blue light can be Rayleigh scattered in the cosmetic film. Therefore, among the visible lights contained in sunlight or artificial light, blue light can be scattered while selectively transmitting red light. And by improving the transmittance of red light, the production of leptin can be enhanced, and by the reflection of blue light, the transparency of the skin can be improved. With the cosmetic of the present invention, it becomes possible to achieve both the enhancement of leptin production, and thus anti - aging care, and the improvement of skin transparency, making full use of light without waste during activities outdoors or living indoors.

[0033] The cosmetic of the present invention contains silica particles. Silica is also called anhydrous silicic acid. The silica particles can have a nano-size while maintaining the transparency of the cosmetic film. The average particle diameter of the silica particles is 10 nm to 100 nm, preferably 10 nm to 50 nm, more preferably 10 nm to 30 nm, and even more preferably 10 nm to 20 nm. Within the above range, since the size is smaller than the wavelength of blue light (light with a wavelength of 400 nm to 500 nm), blue light can be Rayleigh scattered, and the transmittance of red light can be improved.

[0034] In the cosmetic of the present invention, the "average particle diameter" specifically means the volume average particle diameter of the primary particles. For example, it can be calculated by obtaining a transmission electron microscope (TEM) image and performing image analysis on it. In addition, the average particle diameter of the secondary particles of the silica particles is not particularly limited, but is preferably 50 nm to 250 nm, more preferably 100 nm to 200 nm. The average particle diameter of the secondary particles means the volume average particle diameter of the secondary particles, and can be calculated, for example, by obtaining a transmission electron microscope (TEM) image and performing image analysis on it.

[0035] In the cosmetic of the present invention, the silica particles with an average particle diameter of 10 nm to 100 nm are not particularly limited by the shape such as spherical, hemispherical, needle-like, plate-like, or the particle structure such as porous, non-porous, hollow, etc. Further, for example, either so-called dry silica particles produced by vapor phase oxidation of silicon halide or so-called wet silica particles produced from water glass or the like may be used. The silica particles with an average particle diameter of 10 nm to 100 nm in the present invention can be used singly or in combination of two or more. Preferred silica particles include dry silica such as fumed silica. Examples of dry silica particles include Aerosil series (Nippon Aerosil Co., Ltd.), CAB-O-SIL series (Cabot Corporation), HDK series (Asahi Kasei Wacker Silicone Co., Ltd.), and examples of wet silica powder include commercially available products such as Nipsil series (Tosoh Silica Corporation), HI-SIL series (PPG), etc.

[0036] In the cosmetic of the present invention, at least a part of the surface of the silica particles with an average particle diameter of 10 nm to 100 nm is preferably hydrophobized. Such hydrophobized silica particles can be prepared by reacting silica particles with a hydrophobizing agent and adding a hydrophobic group to the hydroxyl groups present on the surface of the powder by covalent bond, or by coating the surface of the silica particles by physically adhering a hydrophobizing agent. By the hydrophobization treatment, the silica particles obtain amphiphilic properties.

[0037] Examples of the hydrophobization treatment include methods of treating metal oxide powders with hydrophobizing agents such as organosilicon compounds, silicones, hydrocarbon oils, fatty acids, fatty acid amides, fatty acid esters, higher alcohols, polyoxyalkylene compounds, etc. Particularly preferably, silica particles are treated with an organosilicon compound as a hydrophobizing agent.

[0038] Examples of the organosilicon compound include hexamethyldisilazane, monomethylsilane, dimethylsilane, trimethylsilane, trimethylethoxysilane, isobutyltrimethoxysilane, trimethylchlorosilane, dimethyldichlorosilane, methyltrichlorosilane, dimethylethoxysilane, dimethyldimethoxysilane, diphenyldiethoxysilane, hexamethyldisiloxane, palmitylsilane, etc. These may be used alone or as a mixture of two or more kinds.

[0039] In a preferred embodiment of the cosmetic of the present invention, particularly preferably, silica particles are treated with trimethylsilane, and partially hydrophobized silica having a trimethylsilyl group added to the surface is used.

[0040] In the cosmetic of the present invention, the content of silica particles having an average particle diameter of 10 nm to 100 nm is preferably 0.50% by mass to 8.00% by mass, more preferably 0.70% by mass to 7.00% by mass, still more preferably 0.80% by mass to 6.00% by mass, and even more preferably 0.90% by mass to 5.50% by mass.

[0041] The cosmetic of the present invention contains an ultraviolet scattering agent. The ultraviolet scattering agent is not particularly limited, and examples thereof include fine particle metal oxides having a function of scattering ultraviolet rays, such as titanium oxide, titanium dioxide, zinc oxide, zirconium oxide, cerium oxide, etc. One or more kinds of ultraviolet scattering agents can be used in the cosmetic of the present invention.

[0042] The fine particle metal oxide has a particle diameter called fine particles in the technical field, and for example, the primary particle diameter observed by electron microscopy is usually 5 nm to 100 nm, preferably 10 nm to 80 nm.

[0043] The ultraviolet scattering agent used in the present invention can be prepared by a conventional method such as thermal decomposition of the corresponding metal salt in the gas phase but commercially available products can also be used.

[0044] The ultraviolet scattering agent may have at least a part of its surface coated with an inorganic compound such as silica or alumina, a surfactant such as stearic acid, isostearic acid, or sodium alginate, or an organic compound such as a silicone compound such as silicone oil.

[0045] The content of the ultraviolet scattering agent in the cosmetic of the present invention is 0.50% by mass or less, preferably 0.20% by mass or less, more preferably 0.15% by mass or less, and still more preferably 0.10% by mass or less. Generally, when an ultraviolet scattering agent is contained, the cosmetic film tends to become white and opaque. However, by setting the content of the ultraviolet scattering agent to 0.50% by mass or less, it becomes easier to form a transparent cosmetic film, so the transmission amount of visible light including red light increases, and the production of leptin by red light is enhanced. Further, the content of the ultraviolet scattering agent in the cosmetic of the present invention is preferably 0.00% by mass or more, more preferably 0.03% by mass or more, and still more preferably 0.05% by mass or more.

[0046] The cosmetic of the present invention contains an oily component containing an ultraviolet absorber. The ultraviolet absorber plays a role in protecting against ultraviolet rays and also plays a role in dispersing powder in the cosmetic film. The ultraviolet absorber is not particularly limited, and examples thereof include cinnamic acid-based ultraviolet absorbers, benzophenone-based ultraviolet absorbers, benzoic acid-based ultraviolet absorbers, salicylic acid-based ultraviolet absorbers, dibenzoylmethane-based ultraviolet absorbers, anthranilic acid-based ultraviolet absorbers, urocanic acid-based ultraviolet absorbers, triazine-based ultraviolet absorbers, and the like. Specifically, cinnamic acid-based ultraviolet absorbers such as 2-ethylhexyl paramethoxycinnamate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, glyceryl mono-2-ethylhexanoate diparamethoxycinnamate, octyl methoxycinnamate, methyl diisopropylcinnamate, etc., 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfuric acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfate, 2,4-dihydroxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone, 2,2',4,Benzophenone-based UV absorbers such as 4'-tetrahydroxybenzophenone and 2-hydroxy-4-n-octoxybenzophenone; benzoic acid-based UV absorbers such as para-aminobenzoic acid, ethyl para-aminobenzoate, butyl para-aminobenzoate, 2-ethylhexyl para-dimethylaminobenzoate, glyceryl para-aminobenzoate, octyl para-dimethylaminobenzoate, amyl para-aminobenzoate, and hexyl diethylaminohydroxybenzoyl benzoate; salicylic acid-based UV absorbers such as 2-ethylhexyl salicylate, triethanolamine salicylate, homomenthyl salicylate, dipropylene glycol salicylate, methyl salicylate, ethylene glycol salicylate, phenyl salicylate, amyl salicylate, benzyl salicylate, isopropylbenzyl salicylate, and potassium salicylate; dibenzoylmethane-based UV absorbers such as 4-tert-butyl-4'-methoxydibenzoylmethane, 4-isopropyldibenzoylmethane, 4-methoxydibenzoylmethane, and 4-tert-butyl-4'-hydroxydibenzoylmethane; anthranilic acid-based UV absorbers such as menthyl anthranilate; urocanic acid-based UV absorbers such as ethyl urocanate; triazine-based UV absorbers such as bis(ethylhexyl)oxyphenol methoxyphenyl triazine and ethylhexyl triazone, etc. As the UV absorber, commercially available products can also be used., One or more UV absorbers can be used.,

[0047] The content of the ultraviolet absorber in the cosmetic of the present invention is 8.00% by mass or more, preferably 10.00% by mass or more, and more preferably 12.00% by mass or more. By setting the content of the ultraviolet scattering agent to 0.50% by mass or less while setting the content of the ultraviolet absorber to 8.00% by mass or more, it is possible to obtain a sufficient ultraviolet protection effect while maintaining the transparency of the cosmetic film. Further, the content of the ultraviolet absorber in the cosmetic of the present invention is preferably 30.00% by mass or less, more preferably 25.00% by mass or less, and even more preferably 20.00% by mass or less.

[0048] The cosmetic of the present invention may contain an oily component other than the ultraviolet absorber. The oily component plays a role of dispersing the powder in the cosmetic film. The oily component other than the ultraviolet absorber in the present invention is not particularly limited, and examples thereof include hydrocarbons, higher alcohols, higher fatty acids, esters, silicone oils, vegetable oils, and animal oils, and one or more of them can be used.

[0049] Examples of the hydrocarbon include squalane, squalene, mineral oil, liquid paraffin, light isoparaffin, dodecane, tetradecane, ozokerite, microcrystalline wax, ceresin, α-olefin oligomer, polybutene, hydrogenated polyisoparaffin, limonene, turpentine oil, petrolatum, and the like.

[0050] Examples of the higher alcohol include linear or branched alcohols having 8 to 24 carbon atoms such as coconut oil alcohol, capryl alcohol, capric alcohol, lauryl alcohol, isostearyl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, arachyl alcohol, behenyl alcohol, oleyl alcohol, isostearyl alcohol, hexyl decanol, 2-octyldodecanol, decyltetradecanol, batyl alcohol; sterols such as cholesterol and phytosterol.

[0051] Examples of the higher fatty acids include those having 8 to 24 carbon atoms, such as coconut oil fatty acid, isostearic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, undecylenic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, oxystearic acid, palmitoleic acid, ricinoleic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), isostearic acid, 12-hydroxystearic acid, and the like.

[0052] Examples of the esters include, for example, esters of linear fatty acids and lower alcohols, such as isopropyl myristate, isopropyl palmitate, ethyl oleate; esters of linear fatty acids and linear higher alcohols, such as hexyl laurate, myristyl myristate, decyl oleate, stearyl stearate; esters of linear fatty acids and branched alcohols, such as octyldodecyl myristate, isostearyl palmitate, ethylhexyl stearate, ethylhexyl palmitate; esters of branched fatty acids and lower alcohols, such as ethyl isostearate, isopropyl isostearate; esters of branched fatty acids and linear higher alcohols, such as cetyl ethylhexanoate, hexyl isostearate; esters of fatty acids and polyhydric alcohols, such as PG dicaprylate, triethylhexanoin, glyceryl tri(capryl / capric acid); esters of branched fatty acids and branched alcohols, such as 2-octyldodecyl neopentanoate, isostearyl isostearate; esters of hydroxycarboxylic acids and alcohols, such as lauryl lactate, trioctyldodecyl citrate, diisostearyl malate; ester oils such as esters of dibasic acids, such as diisopropyl adipate, diethyl sebacate. Examples of wax esters include jojoba oil, carnauba wax, candelilla wax, rice bran wax, shellac, lanolin, beeswax, montan wax, whale wax, orange raffia oil, sugarcane wax, palm wax, insect wax, wool wax, and the like.

[0053] Examples of silicone oils include dimethylpolysiloxane, methylphenylpolysiloxane, dodecamethylcyclohexasiloxane, methylhydrogenpolysiloxane, dimethylsiloxane, and the like.

[0054] Examples of vegetable oils include almond oil (almond kernel oil), camellia oil, argan oil, soybean oil, olive oil, castor oil, coconut oil, palm oil, palm kernel oil, sesame oil, perilla oil, jojoba oil (jojoba seed oil), cottonseed oil, rapeseed oil, linseed oil, rosehip oil, sunflower oil, essential oil, avocado oil, sweet almond oil, rice bran oil, safflower oil, corn oil, grape seed oil, palm oil, argania spinosa kernel oil, wheat germ oil, rice germ oil, kukui nut oil, cranberry vaccinium macrocarpon seed oil, hemp seed oil, peanut oil, camellia oil, evening primrose oil, pistachio oil, macadamia nut oil, meadowfoam oil, cocoa butter, shea butter, candlenut, and other vegetable fats and oils.

[0055] Examples of animal oils include emu oil, horse oil, beef tallow, lard, mutton fat, mink oil, egg yolk fat oil, koi fat, tuna fat, menhaden fat, and other animal fats and oils.

[0056] The content of the oily component (including the content of the ultraviolet absorber) in the cosmetic of the present invention is not particularly limited, but is preferably 10.00% by mass to 40.00% by mass, more preferably 15.00% by mass to 35.00% by mass, and still more preferably 20.00% by mass to 30.00% by mass.

[0057] The cosmetic of the present invention contains water. The water content in the cosmetic of the present invention is not particularly limited, but is preferably 40.00% by mass to 90.00% by mass, more preferably 45.00% by mass to 85.000% by mass, still more preferably 50.00% by mass to 80.00% by mass, and even more preferably 55.00% by mass to 70.00% by mass.

[0058] The cosmetic of the present invention preferably further contains a Saururus chinensis extract. The Saururus chinensis extract has an effect of synergistically enhancing leptin production by red light. The description regarding the Saururus chinensis extract is incorporated by reference to the description in the first aspect.

[0059] The content of the Saururus chinensis extract in the cosmetic of the present invention is not particularly limited, but in terms of solid content, it is preferably 0.0001% by mass to 1.00% by mass, more preferably 0.001% by mass to 0.70% by mass, still more preferably 0.01% by mass to 0.50% by mass, and even more preferably 0.10% by mass to 0.30% by mass.

[0060] The cosmetic of the present invention may further contain a film-forming agent. The film-forming agent is not particularly limited, but examples include silicified pullulan, trimethylsiloxysilicic acid, dimethylaminomethacrylate quaternized salt, vinylpyrrolidone / methacrylic acid-N,N-dimethyl-ethylammonioethyl salt copolymer, silicone / polyether-based polyurethane resin, (methacryloyloxyethyl carboxybetaine / methacrylic alkyl) copolymer, dextrin, (vinylpyrrolidone / VA) copolymer, acrylic acid alkyl copolymer ammonium, polyvinyl alcohol, polyethyl acrylate, (acrylic acid alkyl / octyl acrylate mid) copolymer, (acrylates / methacrylic acid propyltrimethicone) copolymer, polyvinyl acetate, (acrylic acid alkyl / dimethyl silicone) copolymer, polyether graft acrylic silicone, trimethylsiloxysilicic acid, fluorine-modified silicone resin, and the like.

[0061] The content of the film-forming agent in the cosmetic of the present invention is not particularly limited, but can be, for example, 0.20% by mass to 3.00% by mass, or 0.50% by mass to 2.50% by mass.

[0062] The cosmetic of the present invention may further contain a surfactant. The surfactant is not particularly limited, and examples thereof include anionic surfactants such as fatty acid soaps (sodium laurate, sodium palmitate, etc.), potassium lauryl sulfate, and triethanolamine alkyl sulfate ethers; cationic surfactants such as stearyltrimethylammonium chloride, benzalkonium chloride, and laurylamine oxide; imidazoline-based amphoteric surfactants (2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy 2 sodium salt, etc.), betaine-based surfactants (alkyl betaine, amide betaine, sulfobetaine, etc.), and amphoteric surfactants such as acylmethyl taurine; sorbitan fatty acid esters (sorbitan monostearate, sorbitan sesquioleate, etc.), glycerin fatty acids (glycerin monostearate, etc.), propylene glycol fatty acid esters (propylene glycol monostearate, etc.), hydrogenated castor oil derivatives, glycerin alkyl ethers, POE sorbitan fatty acid esters (POE sorbitan monooleate, polyoxyethylene sorbitan monostearate, etc.), POE sorbit fatty acid esters (POE-sorbit monolaurate, etc.), POE glycerin fatty acid esters (POE-glycerin monoisostearate, etc.), POE fatty acid esters (polyethylene glycol monooleate, POE distearate, etc.), POE alkyl ethers (POE 2-octyldodecyl ether, etc.), POE alkyl phenyl ethers (POE nonylphenyl ether, etc.), pluronic types, POE·POP alkyl ethers (POE·POP 2-decyltetradecyl ether, etc.), tetronic types, POE castor oil·hydrogenated castor oil derivatives (POE castor oil, POE hydrogenated castor oil, etc.), sucrose fatty acid esters, alkyl glucosides, and other nonionic surfactants; and the like.

[0063] The content of the surfactant in the cosmetic of the present invention is not particularly limited, but for example, it can be 0.10 to 5.00% by mass, 0.30% to 3.00% by mass, or 0.50% to 1.00% by mass.

[0064] The cosmetic of the present invention may further contain a thickener. The thickener is not particularly limited, and examples thereof include guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, chondroitin sulfate, dermatan sulfate, glycogen, heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, kerato sulfate, locust bean gum, succinoglucan, carron acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, polyethylene glycol and the like.

[0065] The content of the thickener in the cosmetic of the present invention is not particularly limited, but for example, it can be 0.01 to 3.00% by mass, 0.05% to 1.00% by mass, or 0.10% to 0.50% by mass.

[0066] The cosmetic of the present invention may further contain titanium oxide having an average particle diameter of 0.5 μm or more. Such titanium oxide has an excellent function of scattering near-infrared rays and imparts a near-infrared ray protection effect to the cosmetic of the present invention. The average particle diameter of the titanium oxide is more preferably 0.8 μm to 2.0 μm, and still more preferably 1.0 μm to 1.5 μm.

[0067] The titanium oxide in the present invention having an average particle diameter of 0.5 μm or more is preferably surface-treated (coated) with an inorganic compound such as silica or alumina, a surfactant such as stearic acid, isostearic acid, or sodium alginate, or an organic compound such as a silicone compound like silicone oil on at least a part of its surface. When performing the surface treatment, it can be carried out by known surface treatment methods.

[0068] The titanium oxide in the present invention having an average particle diameter of 0.5 μm or more is not particularly limited in other respects as long as it has the above-specified average particle diameter. Such titanium oxide may be a commercially available product. Alternatively, titanium oxide obtained by surface-treating commercially available titanium oxide by a known method can be used. Note that the titanium oxide may be blended alone in one kind with different particle sizes, etc. in the cosmetic, or two or more kinds may be combined and blended. The content of titanium oxide having an average particle diameter of 0.5 μm or more in the cosmetic of the present invention is not particularly limited and can be appropriately selected according to the purpose.

[0069] The total content of the powder in the cosmetic of the present invention is 8.50% by mass or less, preferably 8.00% by mass or less, more preferably 7.50% by mass or less. When the total content of the powder is 8.50% by mass or less, the reflection of visible light by the powder can be suppressed, so the transmittance of red light in the cosmetic film increases, and the production of leptin can be enhanced by red light. Also, the total content of the powder in the cosmetic of the present invention is preferably 1.00% by mass or more, preferably 1.50% by mass or more, more preferably 2.00% by mass or more, and still more preferably 2.50% by mass or more. In the present invention, the "powder" includes the above-mentioned silica particles and ultraviolet scattering agent having an average particle diameter of 10 nm to 100 nm, and optionally titanium oxide having an average particle diameter of 0.5 μm or more and other powders contained in the cosmetic.

[0070] The "other powders optionally contained in the cosmetic" in the present invention is not particularly limited. For example, inorganic powders (e.g., talc, kaolin, mica, sericite, muscovite, phlogopite, synthetic mica, red mica, biotite, vermiculite, magnesium carbonate, calcium carbonate, aluminum silicate, barium silicate, calcium silicate, magnesium silicate, strontium silicate, metal tungstate salts, magnesium, silica other than silica particles with an average particle diameter of 10 nm to 100 nm, alumina, zeolite, barium sulfate, calcined calcium sulfate (calcined gypsum), calcium phosphate, fluorapatite, hydroxyapatite, ceramic powder, boron nitride, etc.); organic powders (e.g., polyamide resin powder (nylon powder), polyethylene powder, poly(meth)acrylate methyl powder, polystyrene powder, copolymer resin powder of styrene and acrylic acid, silicone resin powder, benzoguanamine resin powder, polytetrafluoroethylene powder, cellulose powder, etc.); metal soaps ( For example, zinc myristate, calcium palmitate, aluminum stearate); non- For example, zinc myristate, calcium palmitate, aluminum stearate); non- For example, zinc myristate, calcium palmitate, aluminum stearate); non- Machine white pigments (e.g., zinc oxide, etc.); inorganic red pigments (e.g., iron oxide (red iron oxide), iron titanate, etc.); inorganic brown pigments (e.g., γ-iron oxide, etc.); inorganic yellow pigments (e.g., yellow iron oxide, loess, etc.); inorganic black pigments (e.g., black iron oxide, etc.); inorganic purple pigments (e.g., manganese violet, cobalt violet, etc.); inorganic green pigments (e.g., chromium oxide, chromium hydroxide, cobalt titanate, etc.); inorganic blue pigments (e.g., ultramarine, dark blue, etc.); pearl pigments (e.g., titanium oxide-coated mica, titanium oxide-coated bismuth oxychloride, titanium oxide-coated talc, colored titanium oxide-coated mica, bismuth oxychloride, fish scale foil, etc.); metal powder pigments (e.g., aluminum powder, copper powder, etc.); organic pigments such as zirconium, barium or aluminum lake (e.g., organic pigments such as Red No. 201, Red No. 202, Red No. 204, Red No. 205, Red No. 220, Red No. 226, Red No. 228, Red No. 405, Orange No. 203, Orange No. 204, Yellow No. 205, Yellow No. 401, and Blue No. 404, etc., Red No. 3, Red No. 104, Red No. 106, Red No. 227, Red No. 230, Red No. 401, Red No. 505, Orange No. 205, Yellow No. 4, Yellow No. 5, Yellow No. 202, Yellow No. 203, Green No. 3 and Blue No. 1, etc.); natural pigments (e.g., chlorophyll, β-carotene, etc.); organically modified clay minerals (e.g., organically modified hectorite, etc.) and the like can be mentioned. Also, its shape is not particularly limited, but generally spherical, hemispherical, needle-shaped, plate-shaped used in cosmetics can be mentioned, and it is not particularly limited by particle structures such as porous, non-porous, hollow, etc. Also, its particle size may be generally the degree used in cosmetics. In the present invention, "other powders optionally contained in cosmetics" can be used singly or in combination of two or more.

[0071] The cosmetic of the present invention can maintain the transparency of the cosmetic film and scatter blue light by Rayleigh scattering while transmitting red light, so it can improve the transmittance of red light, and thus enhance the production of leptin, and at the same time improve the transparency of the skin by the reflection of blue light. Therefore, the cosmetic of the present invention is preferably for enhancing leptin production.

[0072] The cells in which the production of leptin is enhanced by the cosmetic of the present invention are not particularly limited as long as they are cells that originally produce leptin, but fibroblasts are preferred.

[0073] In addition, it has been reported that leptin enhances the production and proliferation of type I collagen in fibroblasts (Li P et al., Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 2005 Jan;19(1):20-22.). Therefore, the cosmetic of the present invention can be used for enhancing collagen production, and by enhancing the production of collagen, it can also be used for preventing or improving wrinkles and / or sagging.

[0074] The cosmetic of the present invention includes the form of quasi-drugs. As forms of the cosmetic of the present invention, skincare cosmetics, sunscreen cosmetics, and makeup cosmetics are more preferably mentioned. As skincare cosmetics, emulsions, essences, beauty liquids, etc. are preferably mentioned. As sunscreen cosmetics, those for the face, body, etc. are acceptable. As makeup cosmetics, makeup bases, foundations, concealers, BB creams, CC creams, mascaras, eye colors, eyeliners, blushes, eyebrow colors, etc. are preferably mentioned.

[0075] As the dosage form of the cosmetic, for example, lotion dosage form, emulsion dosage forms such as emulsions and creams, oil dosage form, gel dosage form, powder dosage form, etc. can be mentioned, and it is not particularly limited.

[0076] The cosmetic of the present invention can be produced according to a conventional method.

[0077] The cosmetic of the present invention can optionally contain components normally used in cosmetics, as long as the effects of the present invention are not impaired, in addition to the components described above.

[0078] Examples of such components include lower alcohols such as ethanol and isopropanol; vitamins such as vitamin A or its derivatives, vitamin B6 hydrochloride, vitamin B6 tripalmitate, vitamin B6 dioctanoate, vitamin B2 or its derivatives, vitamin B 12 , vitamin B 15 or derivatives thereof; vitamin E such as α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E acetate, etc., vitamin D, vitamin H, pantothenic acid, pantethine, pyrroloquinoline quinone, etc.; glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, allantoin, etc.; anti-inflammatory agents; wrinkle-improving agents such as retinol, ascorbic acid, tocopherol, or farnesyl acetate; various extracts (e.g., Paeonia lactiflora, Coptis japonica, Citrus unshiu, Peony, Ginseng, Betula, Sage, Loquat, Carrot, Aloe, Japanese butterbur, Iris, Grape, Dioscorea batatas, Bottle gourd, Lily, Saffron, Senkyu, Ginger, Scutellaria baicalensis, Ononis spinosa, Garlic, Capsicum annuum, Zanthoxylum piperitum, Cornus officinalis, seaweed, etc.); activators such as royal jelly, photosensitizer, cholesterol derivatives, etc.; blood circulation promoters such as nonyl vanillylamide, capsaicin, gingerol, tannic acid, etc.; anti-seborrheic agents such as sulfur, thiol, etc.; anti-inflammatory agents such as thiotaurine, hypotaurine, etc.; water-soluble polymers such as collagen, hyaluronic acid, etc.; and the like.

[0079] In addition, various active ingredients, moisturizers, preservatives, antibacterial agents, antioxidants and antioxidant aids, etc. can be optionally formulated in the cosmetics of the present invention.

[0080] Examples of the active ingredients include whitening ingredients, wrinkle-improving ingredients, anti-inflammatory ingredients, extracts derived from animals and plants, etc. Examples of the whitening ingredients include 4-n-butylresorcinol, glucosyl ascorbic acid, 3-О-ethylascorbic acid, arbutin, ellagic acid, kojic acid, linoleic acid, nicotinamide, 5,5'-dipropylbiphenyl-2,2'-diol, 5'-adenylic acid disodium lithium, potassium 4-methoxysalicylate, hydroquinone, etc.

[0081] As for the wrinkle-improving ingredient, there is no particular limitation as long as it is generally used in cosmetics. For example, sodium isopropyl oxopropyl aminocarbonyl pyrrolidine carbonyl methylpropyl aminocarbonyl benzoyl aminoacetate, nicotinamide, vitamin A or its derivatives (retinol, retinal, retinoic acid, tretinoin, isotretinoin, retinoic acid tocopherol, retinol palmitate, retinol acetate, etc.), benzyl ester of ursolic acid, phosphate ester of ursolic acid, benzyl ester of betulinic acid, phosphate ester of benzoic acid can be mentioned.

[0082] Examples of the anti-inflammatory ingredient include clarinone, glabridin, glycyrrhizic acid, glycyrrhetinic acid, pantothenyl alcohol, etc. Preferably, they are glycyrrhizic acid and its salts, alkyl glycyrrhetinate and its salts, and glycyrrhetinic acid and its salts.

[0083] The extracts derived from animals and plants are not particularly limited as long as they are generally used in pharmaceuticals, cosmetics, foods, etc. For example, Akebia extract, Asnaro extract, Asparagus extract, Avocado extract, Amacha extract, Almond extract, Arnica extract, Aloe extract, Aronia extract, Apricot extract, Ginkgo extract, Indian Quince extract, Wikstroemia extract, Udo extract, Gynostemma extract, Hokkaido Butterbur extract, Glehnia extract, Gold extract, Bigleaf Magnolia extract, Oren extract, Otane Carrot extract, Hosta extract, Japanese Violet extract, Orange extract, Kakyo extract, Kakkone extract, Chamomile extract, Carrot extract, Wild Mugwort extract, Licorice extract, Kiwi extract, Cucumber extract, Guava extract, Kudzu extract, Gardenia extract, Cape Jasmine extract, Kumazasa extract, Clara extract, Walnut extract, Grapefruit extract, Black Rice extract, Chlorella extract, Mulberry extract, Keiketu extract, Getu extract, Gentiana extract, Gen-noshokou extract, Black Tea extract, Burdock extract, Rice extract, Fermented Rice extract, Rice Bran Fermented extract, Rice Germ Oil, Japanese Plum extract, Sage extract, Soapwort extract, Sasa extract, Japanese Hawthorn extract, Sansho extract, Shiitake extract, Dioscorea extract, Shikon extract, Perilla extract, Japanese Elm extract, Shimosuke Salsify extract, Peony extract, Ginger extract, Iris Root extract, Silver Birch extract, Japanese Thoroughwort extract, Stevia extract, Fermented Stevia, European Honeysuckle extract, European Japanese Hawthorn extract, European Pennywort extract, European Hosta extract, European Mint extract, Sage extract, Zenia Oi extract, Senkyu extract, Senburi extract, Souhakuhi extract, Daiou extract, Soybean extract, Taiso extract, Thyme extract, Dandelion extract, Tea extract, Clove extract, Chinpi extract, Sweet Tea extract, Chili Pepper extract, Dong Quai extract, Chinese Peony extract, Tonin extract, Japanese Red Cedar extract, Houttuynia extract, Tomato extract, Natto extract, Carrot extract, Garlic extract, Rosa multiflora extract, Hibiscus extract, Bakumondou extract, Lotus extract, Parsley extract, Birch extract, Witch Hazel extract, Hikokoshi extract, Hinoki extract, Loquat extract, Fuki Dandelion extract, Fuki-no-tou extract, Bukuryou extractExtracts such as butcher's broom extract, grape extract, grape seed extract, loofah extract, safflower extract, peppermint extract, bodhi tree extract, button extract, hop extract, pine extract, mayonnaise extract, horse chestnut extract, arrowroot extract, mulberry extract, lemon balm extract, mozuku extract, peach extract, eucalyptus extract, snowdrop extract, yuzu extract, lily extract, wild yam extract, mugwort extract, lavender extract, green tea extract, apple extract, rooibos tea extract, jujube extract, lettuce extract, lemon extract, kelp extract, aster extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, and valerian extract are preferably mentioned.

[0084] Examples of humectants include polyethylene glycol, propylene glycol, glycerin, 1,3-butylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucitin sulfate, caronic acid, atelocollagen, cholesteryl-12-hydroxystearate, lactic acid, sodium lactate, bile salts, dl-pyrrolidone carboxylate, short-chain soluble collagen, diglycerin (EO) PO adduct, Rosa multiflora Thunb. extract, Achillea millefolium L. extract, and melilot extract.

[0085] Examples of pH adjusters include buffers such as lactic acid - sodium lactate, citric acid - sodium citrate, and succinic acid - sodium succinate. Examples of preservatives include methylparaben, ethylparaben, butylparaben, and phenoxyethanol. Examples of antibacterial agents include synthetic ones such as 1,3-butylene glycol and paraoxybenzoic acid esters, as well as natural antibacterial substances such as caprylyl glycol, glyceryl caprylate, ethylhexylglycerin, and caprylic hydroxamic acid. Examples of the antioxidant include tocopherols, dibutylhydroxytoluene, butylhydroxyanisole, gallic acid esters, and the like. Examples of the antioxidant aid include phosphoric acid, citric acid, ascorbic acid, maleic acid, malonic acid, succinic acid, fumaric acid, cephalin, hexametaphosphate, phytic acid, ethylenediaminetetraacetic acid, and the like.

Example

[0086] Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to the following examples as long as the gist thereof is not exceeded.

[0087] <Reference Example 1> Yagurumagiku extract was prepared according to the following procedure. The flower heads of dried Yagurumagiku (Asteraceae, Yagurumagiku) were finely chopped, and 5 times the amount of a 50% aqueous solution of 1,3-butylene glycol was added for extraction.

[0088] <Test Example 1> Examination of the effect of Yagurumagiku extract on the leptin production amount in fibroblasts Normal human dermal fibroblasts (NHDF) (Kuraray Co., Lot No. 05884, newborn, Asian / Caucasian) stored at -150 °C were revived and cultured for 5 days in an environment of 37 °C and 5% CO2. The recovered NHDF was diluted with Dulbecco's Modified Eagle's Medium-high glucose (DMEM) medium (GIBCO, catalog number 11965-092), and 500 μL / well was seeded in a 24-well plate so as to be 4.0×10 4 cells / well, and cultured overnight in an environment of 37 °C and 5% CO2.

[0089] After culturing, the medium in the 24-well plate was removed, and an equal amount of 1% containing 0.2% Yagurumagiku extract Fetal bovine serum (FBS) / DMEM medium was added, and the cells were cultured at 37 °C in a 5% CO2 environment for 48 hours. As a control, 1% FBS / DMEM medium containing 0.2% 1,3-butylene glycol was added.

[0090] After culturing, the culture supernatant was collected, and the amount of leptin in the culture supernatant was measured by ELISA using a Human Leptin ELISA Kit (Abcam, ab179884) according to the attached protocol. In addition, the cell count was measured using a Cell Counting Kit-8 (DOJINDO, 341-07621), and the leptin production amount per unit cell was calculated.

[0091] The results are shown in Figure 1. As shown in Figure 1, no significant increase in the amount of leptin was shown by the yarrow extract alone compared to the control.

[0092] <Test Example 2> Examination of the effects of yarrow extract and red light on leptin production in fibroblasts Normal human dermal fibroblasts (NHDF) (Kurabo, Lot No. 05884, neonatal, Asian / Caucasian) stored at -150 °C were revived and cultured in a 37 °C, 5% CO2 environment for 5 days. The recovered NHDF was diluted with Dulbecco's Modified Eagle's Medium-high glucose (DMEM) medium (GIBCO, catalog number 11965-092), and 500 μL / well was seeded in a 24-well plate at a density of 4.0×10 4 cells / well and cultured overnight in a 37 °C, 5% CO2 environment.

[0093] After culturing, the medium in the 24-well plate was removed, washed with PBS(-), and then 300 μ L / well of fresh PBS(-) was added, and light emitted from a red LED with a peak wavelength of 625 nm was irradiated at 45 W / m 2 2, at 0.88 J / cm 2Irradiation was performed under the conditions described above. As Control 1, no such irradiation was performed, and in Control 2, the irradiation was performed. After the irradiation, the PBS(-) in the 24-well plate was removed, and an equal volume of 1% fetal bovine serum (FBS) / DMEM medium containing 0.2% Saururus chinensis extract was added, followed by culturing for 48 hours in an environment of 37°C and 5% CO2.

[0094] For Controls 1 and 2, 1% FBS / DMEM medium containing 0.2% 1,3-butylene glycol was added. After culturing, the culture supernatant was collected, and the amount of leptin in the culture supernatant was measured by ELISA using a Human Leptin ELISA Kit (Abcam, ab179884) according to the attached protocol.

[0095] Also, the cell count was measured using a Cell Counting Kit-8 (DOJINDO, 341-07621), and the amount of leptin produced per unit cell was calculated. The results are shown in Figure 2. As shown in Figure 2, when comparing Control 1, which was not irradiated with red light and did not have Saururus chinensis extract added, with Control 2, which was irradiated with red light but did not have Saururus chinensis extract added, the amount of leptin increased in Control 2. From this, it was shown that red light has an effect of enhancing leptin production.

[0096] Moreover, when compared with Controls 1 and 2, the amount of leptin increased significantly when red light irradiation and Saururus chinensis extract were added. From the results of Figures 1 and 2, it was shown that Saururus chinensis extract synergistically enhances leptin production by red light. <Test Example 3> Evaluation of Compatibility between Transmission of Red Light and Rayleigh Scattering of Blue Light

[0097] Cosmetics of the Examples and Comparative Examples were prepared according to the formulations shown in the following table. In the table, the "mixture of BG, water, and Saururus chinensis extract" refers to the Saururus chinensis extract prepared in Reference Example 1. ​

[0098] The transmittance of red light and the Rayleigh scattering rate of blue light of the cosmetic were measured and evaluated as follows. <Measurement and Evaluation of Rayleigh Scattering Rate of Blue Light> The cosmetic was applied to a glass substrate using a 2-mil doctor blade to form a thin film. After drying for 10 minutes, the average reflectance in the wavelength range of 400 nm to 500 nm was measured using an ultraviolet-visible spectrophotometer / integrating sphere. As the evaluation criterion, ○ was used when the average reflectance was 30% or more, and × was used when it was less than 30%. <Measurement and Evaluation of Transmittance of Red Light> The cosmetic was applied to a glass substrate using a 2-mil doctor blade to form a thin film. After drying for 10 minutes, the average transmittance in the wavelength range of 620 nm to 660 nm was measured using an ultraviolet-visible spectrophotometer. As the evaluation criterion, ○ was used when the average transmittance was 30% or more, and × was used when it was less than 30%.

[0099] The evaluation results are shown in the following table.

[0100]

Table 1

Industrial Applicability

[0101] Since the leptin production enhancer according to the first aspect of the present invention has an excellent leptin production enhancing effect, it can be suitably used as a component of cosmetics and the like. Further, since the cosmetic according to the second aspect of the present invention has an excellent leptin production enhancing effect and blue light scattering effect, it can be suitably used as a cosmetic. Further, the cosmetic according to the second aspect of the present invention can also be used for enhancing collagen production and for preventing or improving wrinkles and / or sagging.

Claims

1. A leptin production enhancer containing a parsley extract for enhancing the production of leptin under light irradiation of 620 nm to 770 nm.

2. The leptin production enhancer according to claim 1, which enhances the production of leptin in fibroblasts.

3. The leptin production enhancer according to claim 1 or 2, which is a topical skin preparation.

4. A cosmetic containing silica particles with an average particle diameter of 10 nm to 100 nm, an ultraviolet scattering agent, an oily component containing an ultraviolet absorber, and water, wherein the total content of powders in the cosmetic is 8.50% by mass or less, the content of the ultraviolet scattering agent in the cosmetic is 0.50% by mass or less, and the content of the ultraviolet absorber in the cosmetic is 8.00% by mass or more.

5. The cosmetic according to claim 4, wherein at least a part of the surface of the silica particles is hydrophobically treated.

6. The cosmetic according to claim 4, wherein the content of the silica particles in the cosmetic is 0.50% by mass to 8.00% by mass.

7. The cosmetic according to claim 4, further containing a parsley extract.

8. The cosmetic according to any one of claims 4 to 7, which is for enhancing leptin production.

9. The cosmetic according to any one of claims 4 to 7, which is for enhancing collagen production.

10. The cosmetic according to any one of claims 4 to 7, which is for preventing or improving wrinkles and / or sagging.

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