Insertion tool for preparing tissue specimens and use thereof

Abstract

To provide a method of preparing fixed tissue of a tubular organ, which reduces collapse of a luminal portion.SOLUTION: A method of preparing fixed tissue of a tubular organ according to one aspect of the present invention comprises a step 1 of inserting an insertion tool into a lumen of a tubular organ, and a step 2 of fixing the tubular organ with the insertion tool inserted thereto using a fixative.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an insert for preparing a tissue specimen and its use. [Background technology]

[0002] In the fields of medicine and life science, when preparing thin-section tissue specimens, the specimens are usually fixed, dehydrated, infiltrated with an embedding agent, and embedded. A tissue specimen holder, such as an embedding cassette, is known as a tool used in these processes (see, for example, Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Special table number 2007-508569 Summary of the Invention [Problem to be solved by the invention]

[0004] The type of deformation that tissues undergo during fixation, dehydration, and embedding agent penetration treatments varies depending on the type of tissue. For example, in the case of tubular organs such as the small intestine, the tubes may collapse, narrowing the lumen. When a thin tissue specimen is prepared from such a tubular organ, intraluminal structures such as villi may overlap, making adequate histological evaluation impossible. As an example, referring to the microscopic image in Figure 8, there are areas where the lumen has been narrowed and collapsed, resulting in overlapping of villi on opposing intestinal walls, or overlapping of villi on the intestinal wall and villi on the mucosa (see circled areas). It was difficult to prevent such deformation using the technology disclosed in Patent Document 1.

[0005] An object of one aspect of the present invention is to provide a production method that can reduce collapse of the luminal portion when producing fixed tissue, dehydrated tissue, tissue permeated with an embedding agent, or embedded material of a tubular organ. [Means for solving the problem]

[0006] The present invention includes the following aspects. <1> A method for producing a fixed tissue of a tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2a: A step of fixing the tubular organ with the insertion tool inserted therein with a fixative. <2> 1. A method for producing dehydrated tissue of a tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2b: A step of dehydrating the tubular organ with the insertion tool inserted therein using a dehydrating agent. <3> 1. A method for producing tissue obtained by infiltrating a tubular organ with an embedding agent, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2c: A step of infiltrating an embedding agent into the tubular organ with the insertion tool inserted therein. <4> 1. A method for producing an embedded tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2d: A step of embedding the tubular organ with the insertion tool inserted in an embedding agent. <5> The method further comprises the following step 2d' after the step 2c: <3> The manufacturing method described in Step 2d': A step of embedding the tubular organ in the embedding agent in a state in which the insertion tool has been removed from the lumen of the tubular organ. <6> The tubular organ is the digestive tract. <1> ~ <5> The manufacturing method according to any one of the above. <7> An insertion tool for preparing a tissue specimen, It is configured to be inserted into the lumen of a tubular organ and to suppress deformation of the lumen. Insertion tool. <8> The diameter of the insertion tool is 30 to 95% of the diameter of the lumen of the tubular organ. <7> The insertion tool described in <9> At least a portion of which is made of a soft and / or elastic material; <7> or <8> The insertion tool described in <10> <7> ~ <9> The insertion tool according to any one of Cassette for tissue specimen preparation. <11> The tissue specimen preparation cassette includes a main body, the body and the insert are integrally molded; <10> A cassette for preparing a specimen according to claim 1. <12> The tissue specimen preparation cassette includes a main body and a lid portion that can be opened and closed relative to the main body, the insert has a first end and a second end; a first end of the insertion tool is fixed to an inner bottom surface, an inner side surface, or an inner top surface of the main body; The maximum and minimum lengths of the insertion tool from the first end to the second end are as follows: <10> or <11> Specimen preparation cassettes according to Maximum value: the length at which the second end of the insertion tool abuts against a member facing the inner bottom surface, the inner side surface, or the inner top surface to which the first end of the insertion tool is fixed when the lid part is closed; Minimum: 80% of the maximum length. <13> The tissue specimen preparation cassette includes a main body, The internal space of the main body is divided into a plurality of storage sections, One of the insertion tools is provided for each of the storage sections. <10> ~ <12> 10. A cassette for preparing a tissue specimen according to any one of the preceding claims. [Effects of the Invention]

[0007] According to one aspect of the present invention, there is provided a method for producing fixed tissue, dehydrated tissue, tissue permeated with an embedding agent, or embedded material of a tubular organ, which can reduce collapse of the luminal portion. [Brief explanation of the drawings]

[0008] [Figure 1] 1 is a flow chart illustrating an example of a manufacturing method according to one embodiment of the present invention. [Figure 2] 1 is a schematic diagram illustrating an example of an insertion tool and a tubular organ according to one embodiment of the present invention. [Figure 3] FIG. 2 is a schematic diagram showing a state in which the insertion tool is inserted into the lumen of a tubular organ. [Figure 4] 1 is a schematic diagram illustrating an example of a cassette for preparing a tissue specimen according to one embodiment of the present invention. [Figure 5] FIG. 2 is a schematic diagram showing a state in which the cover of the tissue specimen preparation cassette is closed. [Figure 6] 5 is a schematic diagram showing a cross section of a cassette for preparing tissue specimens, taken along the line AA' in FIG. 5, viewed from the direction of the arrow. [Figure 7] 1 is a micrograph showing a small intestine tissue specimen produced by a method according to one embodiment of the present invention. [Figure 8] 1 is a micrograph showing a small intestine tissue specimen prepared by a method according to the prior art. DETAILED DESCRIPTION OF THE INVENTION

[0009] However, the present invention is not limited to the following embodiments and various modifications may be made within the scope of the claims. Embodiments that combine technical means described in different embodiments are also included in the technical scope of the present invention.

[0010] Unless otherwise specified in this specification, "A to B" representing a numerical range means "greater than or equal to A and less than or equal to B." Unless otherwise specified in this specification, elements with the same reference numerals have the same or similar functions.

[0011] [Embodiment 1] 1 is a flow chart showing an example of a manufacturing method according to one embodiment of the present invention. This flow chart roughly includes the steps of fixing a tubular organ 1 (S2a), dehydrating it (S2b), infiltrating it with an embedding agent (S2c), and embedding it (S2d, S2d'). Corresponding to each of these steps, a fixed tissue, a dehydrated tissue, a tissue infiltrated with an embedding agent, or an embedded tissue is obtained.

[0012] In Figure 1, S1 is followed by S2a, S2b, S2c, and S2d (or S2d'), but it is not essential that all of these steps be performed. In the method for producing fixed tissue, S1 and S2a are required steps, and the other steps are optional. In the method for producing dehydrated tissue, S1 and S2b are required steps, and the other steps are optional. In the method for producing tissue infiltrated with an embedding medium, S1 and S2c are required steps, and the other steps are optional. In the method for producing embedded tissue, S1 and S2d (or S1, S2c, and S2d') are required steps, and the other steps are optional.

[0013] Therefore, the order of S1 is not limited to before fixation of the tubular organ 1. In the method for producing dehydrated tissue, S1 may be performed after fixation of the tubular organ 1 and before dehydration. In the method for producing tissue infiltrated with an embedding agent, S1 may be performed after fixation of the tubular organ 1 and before dehydration, or after dehydration of the tubular organ 1 and before infiltration with an embedding agent. In the method for producing an embedded object, S1 may be performed after fixation of the tubular organ 1 and before dehydration, or after dehydration of the tubular organ 1 and before infiltration with an embedding agent, or after infiltration of the tubular organ 1 with an embedding agent and before embedding in the embedding agent. In addition, the insertion tool 5 may be temporarily removed from the tubular organ 1 during this process.

[0014] However, the fixation, dehydration, and embedding agent infiltration processes are all processes that may deform the tubular organ 1. Therefore, S1 is preferably performed before fixing the tubular organ 1. Furthermore, the fixation, dehydration, and embedding agent infiltration processes are preferably performed with the insertion tool 5 inserted in the tubular organ 1. In other words, the flow shown in Figure 1 is one preferred embodiment.

[0015] [S1 (Process 1)] In S1, an insertion tool 5 is inserted into the lumen of the tubular organ 1. Figure 2 shows a schematic representation of the tubular organ 1 and the insertion tool 5. Figure 3 shows a schematic representation of the insertion tool 5 inserted into the lumen of the tubular organ 1. As shown in Figure 4, the insertion tool 5 may be a component of a cassette 100 for preparing a tissue specimen.

[0016] A tubular organ 1 is an organ having both ends and a tubular wall, which define a lumen. The tubular organ 1 may have holes or protrusions in the tubular wall, or the lumen may be branched. Examples of tubular organs 1 include the digestive tract (esophagus, stomach, small intestine, large intestine, etc.), trachea, blood vessels, vagina, uterus, fallopian tubes, ureters, bladder, and gallbladder. Among these, the small intestine is preferred as the tubular organ 1. This is because, since villous tissue is developed within the lumen of the small intestine, the production method according to one embodiment of the present invention can prevent deformation of the tubular organ 1, such as overlapping of villi.

[0017] The insertion tool 5 is a member for insertion into the lumen of the tubular organ 1. When the insertion tool 5 is inserted into the lumen of the tubular organ 1, deformation of the tubular organ (particularly, depression of the tubular wall) is limited (see Figures 3 and 4). Therefore, deformation that narrows the lumen of the tubular organ 1 can be reduced. Preferred embodiments of the insertion tool 5 will be described later in Examples 2 and 3.

[0018] [S2a (Process 2a)] In S2a, the tubular organ 1 with the insertion tool 5 inserted is fixed with a fixative. The tubular organ 1 with the insertion tool 5 inserted is in the state shown in Figure 3 or 4. The fixation conditions can be determined appropriately by those skilled in the art depending on the type, size, etc. of the tubular organ 1.

[0019] The fixed tissue obtained through S2a may be transferred to S2b with the insertion tool 5 still inserted, or may be subjected to a dehydration process or the like after removing the insertion tool 5. The fixed tissue obtained through S2a can be used to prepare tissue specimens, but its use is not limited to this.

[0020] [S2b (Step 2b)] In S2b, the tubular organ 1 with the insertion tool 5 inserted therein is dehydrated with a dehydrating agent. The tubular organ 1 with the insertion tool 5 inserted therein is in the state shown in Figure 3 or Figure 4. The dehydration conditions can be determined appropriately by those skilled in the art depending on the type, size, etc. of the tubular organ 1.

[0021] The dehydrated tissue obtained through S2b may be transferred to S2c with the insertion tool 5 still inserted, or may be subjected to a permeation treatment with an embedding agent after removing the insertion tool 5. The dehydrated tissue obtained through S2b can be used to prepare tissue specimens, but its use is not limited to this.

[0022] [S2c (process 2c)] In S2c, an embedding agent (such as paraffin) is infiltrated into the tubular organ 1 with the insertion tool 5 inserted. The tubular organ 1 with the insertion tool 5 inserted is in the state shown in Figure 3 or Figure 4. The conditions for infiltrating the embedding agent can be determined appropriately by those skilled in the art depending on the type, size, etc. of the tubular organ 1.

[0023] The embedding medium-infused tissue obtained through S2c may be transferred to S2d with the insertion tool 5 still inserted. In this case, it is preferable to use an insertion tool 5 that is not a component of the tissue preparation cassette 100, as shown in FIG. 3. Alternatively, the embedding medium-infused tissue obtained through S2c may be transferred to S2d after the insertion tool 5 is removed. In this case, it is possible to use either an insertion tool 5 that is not a component of the tissue preparation cassette 100, as shown in FIG. 3, or an insertion tool 5 that is a component of the tissue preparation cassette 100, as shown in FIG. 4. The embedding medium-infused tissue obtained through S2c can be used for preparing tissue preparations, but its use is not limited to this.

[0024] [S2d (Process 2d)] In S2d, the tubular organ 1 with the insertion tool 5 inserted is embedded in an embedding agent (such as paraffin). The tubular organ 1 with the insertion tool 5 inserted is in the state illustrated in Figure 3 or Figure 4 (usually the state illustrated in Figure 3). The embedding conditions can be appropriately determined by those skilled in the art depending on the type, size, etc. of the tubular organ 1.

[0025] The embedded material obtained through S2d can be used, for example, to prepare a slice specimen. In this case, the insertion tool 5 is sliced ​​together with the tubular organ 1.

[0026] [S2d' (Step 2d')] In S2d', the insertion tool 5 is removed from the tubular organ 1, and the tubular organ 1 is then embedded in an embedding agent (such as paraffin). The tubular organ 1 with the insertion tool 5 inserted is in the state shown in Figure 3 or Figure 4. The embedding conditions can be determined appropriately by those skilled in the art depending on the type, size, etc. of the tubular organ 1.

[0027] The embedded material obtained through S2d can be used, for example, to prepare a slice specimen. In this case, the insert 5 is removed when slicing the tubular organ 1, so the slice does not contain the insert 5.

[0028] [Other processes] A manufacturing method according to one embodiment of the present invention can be used to prepare a tissue specimen. Therefore, the manufacturing method may include any step that can be included in the preparation of a tissue specimen. For example, a step of degreasing the tubular organ 1 may be added between the dehydration treatment and the embedding agent infiltration treatment (e.g., between S2b and S2c). Alternatively, a step of slicing the embedded material, a step of removing the embedding agent from the sliced ​​tubular organ 1, a step of staining the sliced ​​tubular organ 1, or the like may be added after the embedding treatment (e.g., after S2d or S2d').

[0029] [Embodiment 2] One aspect of the present invention is an insertion tool 5 for preparing a tissue specimen. The insertion tool 5 will be described below with reference to Figures 2 and 3. In the second embodiment, at least preferred aspects will be described when the insertion tool 5 is not a component of the tissue specimen preparation cassette 100. However, these aspects can also be applied when the insertion tool 5 is a component of the tissue specimen preparation cassette 100.

[0030] Fig. 2 is a schematic diagram showing an insertion tool 5 and a tubular organ 1 according to one embodiment of the present invention. Fig. 3 is a schematic diagram showing the state in which the insertion tool 5 is inserted into the lumen of the tubular organ 1. By inserting the insertion tool 5 into the lumen of the tubular organ 1, deformation of the lumen is suppressed. In order to suppress deformation of the lumen, the shape and material of the insertion tool 5 are appropriately selected. The appropriate shape and material may vary depending on the type and size of the tubular organ 1.

[0031] In one embodiment, the lower limit of the diameter of the insertion tool 5 can be 30% or more, 40% or more, or 50% or more of the diameter of the lumen of the tubular organ 1. In one embodiment, the upper limit of the diameter of the insertion tool 5 can be 95% or less, 90% or less, 85% or less, or 80% or less of the diameter of the lumen of the tubular organ 1. The diameter of the tubular organ referred to here is the diameter of the lumen of the tubular organ 1 before insertion into the insertion tool 5. By setting the diameter of the insertion tool 5 within the above range, the possibility of the insertion tool 5 unintentionally falling off can be reduced. Furthermore, even if the tubular organ 1 contracts, the possibility of the insertion tool 5 compressing the lumen of the tubular organ 1 can be reduced.

[0032] For example, when the tubular organ 1 is the small intestine of a rat, the diameter of the insertion tool 5 may be about 2 to 5 mm. For example, when the tubular organ 1 is the small intestine of a mouse, the diameter of the insertion tool 5 may be about 1 to 4 mm.

[0033] In one embodiment, at least a portion of the insertion tool 5 is made of a soft and / or elastic material. A soft material refers to a material that easily deforms when an external force is applied. An elastic material refers to a material that easily deforms when an external force is applied but returns to its original shape when the external force is removed. Examples of materials that fall into at least one of the soft and elastic categories include sponge, absorbent cotton, and rubber. By configuring the insertion tool 5 in this manner, damage to intraluminal structures such as villous tissue can be reduced even if the lumen of the tubular organ 1 comes into contact with the insertion tool 5. From this perspective, it is preferable that at least the surface of the insertion tool 5 that comes into contact with the lumen of the tubular organ 1 be made of a soft and / or elastic material. In one embodiment, the entire insertion tool 5 is made of a soft and / or elastic material.

[0034] In a mode in which the tubular organ 1 with the insertion tool 5 inserted is embedded, the insertion tool 5 is preferably made of a material that can be thinly sliced ​​using a microtome or the like. The soft and / or elastic materials described above are examples of such materials. From this perspective, it is preferable that at least the entire portion of the insertion tool 5 into which the tubular organ 1 is inserted is made of a soft and / or elastic material. In one embodiment, the entire insertion tool 5 is made of a soft and / or elastic material.

[0035] [Embodiment 3] One aspect of the present invention is a tissue preparation cassette 100 equipped with an insertion tool 5. The tissue preparation cassette 100 will be described below with reference to Figures 4 to 6. The description of the insertion tool 5 described in the second embodiment can also be applied to the third embodiment.

[0036] The tissue specimen preparation cassette 100 comprises a main body 30 and a lid 40. The lid 40 can be opened and closed relative to the main body 30. Figure 4 shows the lid 40 in an open state, and Figure 5 shows the lid 40 in a closed state. At least some of the components of the main body 30 and the lid 40 have a mesh-like pattern of holes formed through them, allowing various fluids (such as fixatives, dehydrating agents, and embedding agents in a fluid state) to flow through them.

[0037] An insertion tool 5 is fixed to the inside of the main body 30. In this specification, the end of the insertion tool 5 fixed to the main body 30 is referred to as the first end, and the end not fixed is referred to as the second end. In Figures 4 and 6, the insertion tool 5 is fixed to the inside bottom surface of the main body 30. However, the insertion tool 5 may also be fixed to the inside side surface of the main body 30 or the lid portion 40, or may be fixed to the inside top surface of the lid portion 40. The insertion tool 5 allows the tubular organ 1 to be inserted, and is designed to prevent deformation of the tubular organ 1 during fixation, dehydration, embedding agent penetration, embedding, and other processes.

[0038] In one embodiment, the insertion tool 5 may be molded integrally with the main body 30. For example, the insertion tool 5 may be manufactured by injection molding using the same mold as the main body 30. With this configuration, no joint is created between the main body 30 and the insertion tool 5, preventing damage to the inside of the lumen of the tubular organ 1 due to getting caught on the joint.

[0039] In one embodiment, the internal space of the main body 30 may be divided into multiple storage sections. In the example shown in FIG. 4, the internal space of the main body 30 is divided into four storage sections by partitions 10. In this case, it is preferable to provide one insertion tool for each of the divided storage sections. With this configuration, multiple tubular organs 1 can be processed within a single tissue specimen preparation cassette 100. Furthermore, because of the presence of the partitions 10, even if a tubular organ 1 becomes detached from the insertion tool 5, the range of movement within the internal space of the tissue specimen preparation cassette 100 is limited, preventing adverse effects such as confusion with tubular organs 1 inserted in other insertion tools 5 or contact and damage to the tubular organ 1.

[0040] When the first end of the insertion tool 5 is fixed to the inner bottom surface of the main body 30, the length of the insertion tool 5 may be 80% or more, 85% or more, or 90% or more of the length to the member that faces the inner bottom surface when the lid part 40 of the tissue specimen preparation cassette 100 is closed. The member that faces the inner bottom surface may be, for example, the inner top surface of the lid part 40. With this configuration, it is possible to prevent the tubular organ 1 from falling off the insertion tool 5.

[0041] When the first end of the insertion tool 5 is fixed to the inner side surface of the main body 30 or the lid 40, the length of the insertion tool 5 may be 80% or more, 85% or more, or 90% or more of the length to the member that faces the inner side surface when the lid 40 of the tissue specimen preparation cassette 100 is closed. The member that faces the inner side surface may be, for example, the inner side surface of the main body 30, the inner side surface of the lid 40, or the partition wall 10. With this configuration, it is possible to prevent the tubular organ 1 from falling off the insertion tool 5.

[0042] When the first end of the insertion tool 5 is fixed to the inner top surface of the lid 40, the length of the insertion tool 5 may be 80% or more, 85% or more, or 90% or more of the length to the member that faces the inner top surface when the lid 40 of the tissue preparation cassette 100 is closed. The member that faces the inner top surface may be, for example, the inner bottom surface of the main body 30. With this configuration, it is possible to prevent the tubular organ 1 from falling off the insertion tool 5.

[0043] An example in which the first end of the insertion tool 5 is fixed to the inner top surface of the main body 30 will be described in detail with reference to FIG. 6. FIG. 6 is a cross-sectional view of the A-A' cross section in FIG. 5, viewed from the direction of the arrow. In one embodiment, the height (h2) of the insertion tool 5 may be 80% or more, 85% or more, or 90% or more of the height (h1) of the internal space when the lid 40 of the tissue specimen preparation cassette 100 is closed. The upper limit of the height (h2) of the insertion tool 5 may be 100% or less of the height (h1) of the internal space. In other words, when the lid 40 of the tissue specimen preparation cassette 100 is closed, the second end of the insertion tool 5 of the main body 30 abuts against the inner top surface of the lid 40. With this configuration, the gap between the second end of the insertion tool 5 and the lid 40 becomes sufficiently narrow when the lid 40 is closed, preventing the tubular organ 1 from falling out of the insertion tool 5.

[0044] 4, the first end of the insertion tool 5 and the partition wall 10 are fixed to the inner bottom surface of the main body 30. However, at least a portion of these members may be fixed to the inner side surface or inner top surface of the main body 30 or the lid portion 40. In the cross-sectional view shown in FIG. 6, the partition wall 10 is depicted as not abutting the lid portion 40 in the closed state, but it may be configured to abut against the lid portion 40. [Example]

[0045] Example 1 A small intestine embedment was prepared by the production method according to one embodiment of the present invention, and a tissue specimen was prepared from the embedment. The specific procedure was as follows. 1. Approximately 15 mm of the small intestine of a rat was excised and an insertion tool (a sponge mass) was inserted into the lumen. 2. A fixative (formalin) was injected into the lumen, and both ends of the tissue fragment were ligated. The tissue fragment was left in this state for about 24 hours to fix it. 3. The tissue pieces with the insertion tool inserted were placed in a specimen preparation cassette and processed in an automatic embedding device. Inside the automatic embedding device, the tissue pieces were treated with a dehydrating agent (100% ethanol) and a degreasing agent (xylene), and the tissue pieces were then infiltrated with the embedding medium (paraffin). Since the insertion tool was inserted into the tissue pieces in step 1, the specimen preparation cassette used in this step did not come equipped with an insertion tool. 4. The tissue piece was removed from the specimen preparation cassette and the insert was removed from the lumen. 5. The tissue piece without the insertion tool was embedded in embedding medium (paraffin). 6. Tissue specimens were prepared from the embedded material according to standard methods.

[0046] Comparative Example 1 The tissue specimen was prepared in the same manner as in Example 1, except that no insertion tool was used.

[0047] 〔result〕 The tissue specimen according to Example 1 was prepared through steps S1, S2a, S2b, S2c, and S2d'. A microscopic image of the resulting tissue specimen is shown in Figure 7. As can be seen from the figure, the resulting tissue specimen maintained a circular shape to some extent, and the narrowing of the lumen was reduced.

[0048] The tissue specimen of Comparative Example 1 was prepared without using any insertion tool and without going through any of the above steps. A microscopic image of the resulting tissue specimen is shown in Figure 8. As can be seen from the figure, the obtained tissue specimen had a narrowed lumen and had areas where the villi overlapped (see the circled areas). [Explanation of symbols]

[0049] 1: Tubular organs 5: Insertion tool 30: Main body 40: Lid 100: Specimen preparation cassette S1: Process 1 S2a: Process 2a S2b: Process 2b S2c: Process 2c S2d: Process 2d S2d': Process 2d'

Claims

1. A method for producing a fixed tissue of a tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2a: A step of fixing the tubular organ with the insertion tool inserted therein with a fixative.

2. 1. A method for producing dehydrated tissue of a tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2b: A step of dehydrating the tubular organ with the insertion tool inserted therein using a dehydrating agent.

3. A method for producing tissue obtained by infiltrating a tubular organ with an embedding agent, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2c: A step of infiltrating an embedding agent into the tubular organ with the insertion tool inserted therein.

4. A method for producing an embedded tubular organ, comprising the steps of: Step 1: inserting an insertion tool into the lumen of the tubular organ; Step 2d: A step of embedding the tubular organ with the insertion tool inserted in an embedding agent.

5. The method according to claim 3, further comprising the following step 2d' after step 2c: Step 2d': A step of embedding the tubular organ in the embedding agent in a state in which the insertion tool has been removed from the lumen of the tubular organ.

6. The tubular organ is the digestive tract. The method according to any one of claims 1 to 4.

7. An insertion tool for preparing a tissue specimen, It is configured to be inserted into the lumen of a tubular organ and to suppress deformation of the lumen. Insertion tool.

8. The diameter of the insertion tool is 30 to 95% of the diameter of the lumen of the tubular organ.

8. The insert of claim 7.

9. At least a portion of which is made of a soft and / or elastic material; 8. The insert of claim 7.

10. The device is provided with an insert according to any one of claims 7 to 9. Cassette for tissue specimen preparation.

11. The tissue specimen preparation cassette includes a main body, the body and the insert are integrally molded; The specimen preparation cassette according to claim 10.

12. The tissue specimen preparation cassette includes a main body and a lid portion that can be opened and closed relative to the main body, the insert has a first end and a second end; a first end of the insertion tool is fixed to an inner bottom surface, an inner side surface, or an inner top surface of the body; 11. The specimen preparation cassette of claim 10, wherein the maximum and minimum lengths of the insertion tool from the first end to the second end are as follows: Maximum value: the length at which the second end of the insertion tool abuts against a member that faces the inner bottom surface, the inner side surface, or the inner top surface to which the first end of the insertion tool is fixed when the lid part is closed; Minimum value: 80% of the maximum value.

13. The tissue specimen preparation cassette includes a main body, The internal space of the main body is divided into a plurality of storage sections, One of the inserts is provided for each of the storage sections. The tissue specimen preparation cassette according to claim 10.

Images