Scan recognition method and system based on marshalling in-line grating

Abstract

The present invention discloses a scan recognition method and a scan recognition system based on a marshalling in-line grating, and the scan recognition system includes a moving device, a detection plate, an excitation light source, a marshalling in-line grating, an optical fiber, and a recognition device. A marshalling in-line grating is formed by arranging one end of a plurality of optical fibers in a line at a marshalling interval, or a marshalling in-line grating is formed on an end face of one end of an optical fiber by photolithography or etching. The detection plate is installed on the moving device, and there are detection objects distributed in a matrix on the detection plate. The detection plate is scanned by the relative movement formed by the marshalling in-line grating and the detection plate, and the light emitted or reflected by the detection object is collected by the marshalling in-line grating and transmitted to the recognition device for photoelectric conversion, thereby realizing the scan recognition of the detection plate. This scan recognition method can dynamically and continuously count the detection objects on the detection plate, thereby shortening the recognition time, improving the detection efficiency, and improving the detection accuracy.

Description

[Technical field]

[0001] The present invention relates to the technical field of detection, realizing digital single molecule scanning, and in particular to a scan recognition method and scan recognition system based on marshalling in-line gratings. [Background technology]

[0002] Single Molecule Detection (SMD) is an ultra-sensitive detection technology that has been rapidly developing in recent years. It refers to measuring and analyzing targets at the single molecule level. It is a new detection method and opens up a new detection field. Single molecule fluorescence detection is the most commonly used method for single molecule detection, and reflects biological information such as interactions between related molecules, enzyme activity, reaction kinetics, molecular conformation, DNA and RNA transcription, and protein folding by marking the changes in various properties of each fluorescent group of biopolymers. Single molecule fluorescence detection has unique application value in chemical analysis, biological analysis, nanomaterial analysis, medical diagnosis, forensic analysis, single cell analysis, and molecular dynamics mechanism research, and has had a great impact on the development of many disciplines and fields. There are three types of single molecule fluorescence detection: photon burst detection, single molecule image recording, and single molecule spectrum mapping. Photon burst detection is the simplest and directly measures the number of photons that burst. Single molecule imaging can indicate the position and luminescence intensity of molecules in the image, and can track and record single molecules in real time. Simoa (Single-molecule Array) technology developed by Quanterix, Inc. of the United States, is currently the most advanced single molecule fluorescence detection technology, and its sensitivity is more than 1000 times that of ELISA technology, and the lower limit of detection reaches fg / mL, realizing effective detection and quantification of single molecules.

[0003] However, the conventional Simoa technology counts the number of luminescent analytes in the image after imaging, which results in high requirements for imaging equipment and slow detection speed, making it difficult to apply and popularize Simoa technology. Therefore, the present application uses a marshalling in-line grating to dynamically and continuously count the luminescent detection objects, thereby realizing digital single-molecule scanning, thereby improving detection accuracy, shortening recognition time, and improving detection efficiency.

[0004] From the results of the patent search, the patents related to this application are mainly the following:

[0005] 1. A Chinese invention patent with application number "202110018302.1", filing date "2021.01.07", publication number "CN112859256A", publication date "2021.05.28", title "Grating coupler position determination and measurement method based on image recognition", applicant "Tianjin University". This invention patent discloses a grating coupler position determination and measurement method based on image recognition, specifically including: (1) determining the position coordinate of a grating coupler on a chip design drawing; (2) mapping and matching the chip design drawing with the dimensions of the chip on the measurement platform, thereby obtaining the position coordinate of the grating coupler on the chip on the measurement platform; (3) using a program to control the movement of the displacement table to realize the micron-level determination of the positions of the coupling optical fiber and the grating coupler; and (4) using a spatial scanning method to realize the sub-100 nanometer level determination of the positions of the coupling optical fiber and the grating coupler, and finding the optimal coupling position to perform chip measurement. However, the patent aligns the optical signal of the grating coupler by moving the optical fiber and scans it point by point, resulting in low detection efficiency.

[0006] 2. A Chinese invention patent with application number "202111571233.3", filing date "2021.12.21", publication number "CN114240755A", publication date "2022.03.25", entitled "Image super-resolution reconstruction method based on the combination of optical fiber bundle and micro-scanning technology", applicant is "Institute of Optoelectronics Technology, Chinese Academy of Sciences". This invention patent discloses an image super-resolution reconstruction method based on the combination of optical fiber bundle and micro-scanning technology, including a collimating lens, an optical fiber bundle, a displacement driver, a photodetector, a high-voltage amplifier, a displacement control and a data collection device. An object is imaged on its image-side focal plane by a collimating lens and received by an optical fiber bundle, and the displacement control device generates a voltage signal, which is amplified by a high-voltage amplifier and then acts on the displacement driver, so that the optical fiber bundle surface array performs a micro-displacement scan on the image formed by the target object, and the scanning step size of the system can be accurately adjusted to achieve a displacement below the diffraction limit of the optical system. The present invention utilizes the characteristics of image transmission through the core of the optical fiber bundle, and combines with micro-scanning technology to break through the limitation that target detection and recognition is affected by the pixel size of the detector, realizes sub-pixel displacement, compensates for the missing information of the cladding, and quickly captures the optical energy information of the output end of the optical fiber bundle by the photodetector array, reduces information loss, and realizes wide-field super-high resolution imaging. However, the patent does not detect the optical signal by scanning, but projects the optical signal onto the optical fiber bundle by convex lens imaging, which results in low detection accuracy.

[0007] 3. A Chinese invention patent with application number "200810201668.7", filing date "2008.10.23", publication number "CN101387527B", publication date "2010.12.15", title "Optical fiber grating sensing demodulator and its application", applicant "Cao Chungen". This invention patent relates to an optical fiber grating sensing demodulator, which includes a high-power ASE broad spectrum light source, a micro-opto-mechanical scanning filtering system, an optical coupler, a photodetector, a logarithmic amplifier circuit, a signal collection amplification and analog-to-digital conversion circuit, and a digital signal processing system, wherein the high-power ASE broad spectrum light source is connected to the micro-opto-mechanical scanning filtering system, the micro-opto-mechanical scanning filtering system is connected to the optical coupler, the optical coupler is connected to the photodetector, and the photodetector is sequentially connected to the logarithmic amplifier circuit, the signal collection amplification and analog-to-digital conversion circuit, and the digital signal processing system. The accuracy of the present invention is greatly improved, the device is based on micro-optical mechanical scanning filtering technology and DSP signal collection and processing technology, the performance is stable and reliable, the measurement accuracy is high, the remote real-time reading of the demodulator can be realized by remotely reading the reflection wavelength of the center of the optical fiber grating, and in the case of the net-like arrangement of the sensing system, the engineering realization is simple and easy to implement. However, the detection of the patent is performed by the fiber Bragg grating sensor, which is different from the scanning recognition method using the marshalling in-line grating in the present application. Summary of the Invention [Problem to be solved by the invention]

[0008] SUMMARY OF THE DISCLOSURE The technical problem to be solved by the present invention is to provide a scan recognition method and a scan recognition system based on marshalling in-line grating in order to address the deficiencies of the prior art. [Means for solving the problem]

[0009] In order to solve the above technical problems, the technical solution of the present invention is as follows: A scan recognition method based on a marshalling in-line grating, the marshalling in-line grating is composed of a plurality of light collecting points arranged in a line at marshalling intervals, and the light collecting points include light transmitting points or reflecting points. The detection object is arranged in a tiled shape on the detection plate, and the detection plate is scanned by the relative movement formed by the marshalling in-line grating and the detection plate, and the light emitted or reflected by the detection object is collected by the light collecting points and transmitted to the recognition device for photoelectric conversion, thereby realizing the scan recognition of the detection plate. The detection object that emits light is dynamically and continuously counted using the marshalling in-line grating, thereby shortening the recognition time, improving the detection efficiency, and improving the detection accuracy. The light collecting points are square, circular, or elliptical.

[0010] In addition, the detection objects are arranged on the detection plate in tiles according to the form of matrix distribution, and the interval between adjacent light collecting points of the marshalling in-line grating is the same as the matrix interval of the detection objects, so that the light collecting points can be aligned with the detection objects, which can reduce the interference caused by misalignment between the light collecting points and the detection objects, and greatly improve the accuracy of scan recognition.

[0011] Furthermore, the light collecting points are arranged at intervals along the grating direction X to form a marshalling in-line grating. The light collecting points in the marshalling in-line grating correspond to a row of detection objects in the matrix on the detection plate. During the relative movement process between the marshalling in-line grating and the detection plate, the marshalling in-line grating sequentially scans the detection objects in each row in the matrix on the detection plate.

[0012] In addition, adjacent light collecting points are arranged in a staggered manner perpendicular to the grating direction. The light collecting points in the marshalling in-line grating correspond to the detection objects in two adjacent rows in the matrix on the detection plate, thereby increasing the distance between adjacent light collecting points, reducing or avoiding the optical interference of the detection objects adjacent to the detection objects aligned with the light collecting points, and improving the detection accuracy.

[0013] Furthermore, the offset distance between adjacent light collecting points is equal to the spacing between adjacent light collecting points, which is the same as the row and column spacing of the detection target, so that the staggered light collecting points in the marshalling in-line grating correspond to the position of the detection target.

[0014] Furthermore, the direction of relative movement between the marshalling in-line grating and the detection plate is perpendicular to the grating direction.

[0015] In addition, the light emitted or reflected by the object to be detected is collected by the marshalling in-line grating and then transmitted to the recognition device through the optical fiber. The marshalling in-line grating is installed at one end of the optical fiber, and the recognition device is installed at the other end of the optical fiber. By transmitting the optical signal through the optical fiber, the position of the recognition device can be flexibly set, making the structure of the scanning recognition system more compact.

[0016] In addition, one end of a plurality of optical fibers is arranged at intervals along the grating direction X to form light collecting points arranged linearly at marshalling intervals, forming a multi-optical fiber in-line grating or a multi-optical fiber staggered grating. Using one end of a plurality of optical fibers as the light collecting points not only simplifies the structure of the scan recognition system, but also reduces the cost.

[0017] Furthermore, light collecting points arranged linearly at marshalling intervals are formed on the end face of one end of a single optical fiber by photolithography, etching or coating to form a single optical fiber in-line grating or a single optical fiber staggered grating. By manufacturing a marshalling in-line grating using one end of a single optical fiber, the number of optical fibers is reduced, further reducing costs.

[0018] The present invention further relates to a scan recognition system for implementing the scan recognition method, the scan recognition system including a detection plate and an excitation light source, the excitation light source irradiates the detection plate. The scan recognition system further includes a marshalling in-line grating, an optical fiber, a moving device, and a recognition device, the marshalling in-line grating is installed at one end of the optical fiber, the recognition device is installed at the other end of the optical fiber, the moving device moves and drives the detection plate or the marshalling in-line grating to form a relative movement between the marshalling in-line grating and the detection plate, the marshalling in-line grating scans the detection plate, the optical signal acquired by scanning is transmitted to the recognition device through the optical fiber, and the recognition device converts the optical signal into an electrical signal, thereby realizing the scan recognition of the detection plate. The marshalling in-line grating is used to dynamically and continuously count the luminous detection object, thereby shortening the recognition time, improving the detection efficiency, and improving the detection accuracy.

[0019] Beneficial effects of the present invention: By using the marshalling in-line grating to dynamically and continuously count the emitting detection object, the recognition time can be shortened, the detection efficiency can be improved, and the detection accuracy can be improved. In addition, by manufacturing the marshalling in-line grating with optical fiber, the structure of the scan recognition system can be simplified, and the cost can be reduced. [Brief description of the drawings]

[0020] [Figure 1]FIG. 1 is a perspective structural schematic diagram of a scan recognition system according to a first embodiment of the present invention; [Diagram 2] FIG. 1 is a schematic front view of a scan recognition system according to a first embodiment. [Diagram 3] FIG. 11 is a perspective structural schematic diagram of a scan recognition system according to a second embodiment of the present invention. [Figure 4] FIG. 11 is a schematic front view of a scan recognition system according to a second embodiment of the present invention. [Diagram 5] FIG. 2 is a schematic diagram of a detection plate. [Figure 6] FIG. 2 is a schematic perspective view of a single optical fiber in-line grating according to a first embodiment of the marshalling in-line grating; [Figure 7] FIG. 11 is a schematic perspective view of a single optical fiber staggered grating according to a second embodiment of the marshalling in-line grating. [Figure 8] FIG. 11 is a schematic perspective view of the structure of a multi-optical fiber in-line grating according to a third embodiment of the marshalling in-line grating. [Figure 9] FIG. 11 is a front schematic diagram of a multi-optical fiber in-line grating according to a third embodiment of the marshalling in-line grating. [Figure 10] FIG. 11 is a schematic perspective view of the structure of a multi-optical fiber staggered grating according to a fourth embodiment of the marshalling in-line grating. [Figure 11] FIG. 11 is a schematic front view of a multi-optical fiber staggered grating according to a fourth embodiment of the marshalling in-line grating. [Figure 12] FIG. 1 is a schematic diagram of the scan recognition process 1. [Figure 13] FIG. 2 is a schematic diagram of the scan recognition process 2. [Figure 14] FIG. 3 is a schematic diagram of the scan recognition process 3. [Figure 15] FIG. 4 is a schematic diagram of the scan recognition process 4. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0021] The present invention will now be further described with reference to specific examples and drawings.

[0022] As shown in FIGS. 1 and 2, a scanning recognition system according to a first embodiment of the present invention includes a moving device 1, a detection plate 2, an excitation light source 3, a marshalling in-line grating 4, an optical fiber 5, and a recognition device 6.

[0023] The object to be detected 201 is diluted in a solution and then distributed and tiled on the detection plate 2 or test band. The marshalling in-line grating 4 is composed of a plurality of light collecting points 401 arranged at intervals along a grating direction X. The light collecting points 401 are square, circular or elliptical.

[0024] The excitation light source 3 and the marshalling in-line grating 4 are installed on the same side of the detection plate 2. The detection plate 2 is installed on the moving device 1, and the marshalling in-line grating 4 may be installed on the moving device 1 to form a relative movement between the detection plate 2 or the test band and the marshalling in-line grating 4. The light emitted by the excitation light source 3 is irradiated onto the detection plate 2, and excites a part of the detection target 201 to which a fluorescent marker is bound, causing it to emit light. When the light collection point 401 is positioned close to the detection target 201, it collects the optical signal emitted by the detection target 201 and transmits it to the recognition device 6 via the optical fiber 5, and the photosensitive tube of the recognition device 6 converts the optical signal collected by the marshalling in-line grating 4 into an electrical signal for calculation or remote display.

[0025] In order to simplify the structure of the detection member, the marshalling in-line grating 4 can be coupled to one end of the optical fiber 5. One end of a plurality of optical fibers 5 can be arranged in a line with a marshalling interval to form a light collection point 401 arranged in a line with a marshalling interval, and a multi-optical fiber in-line grating 41 or a multi-optical fiber staggered grating 42 can be formed, and the diameter Q of the optical fiber should be within a range of 0.5 to 1.5 times the maximum dimension d of the detection object. The end face of one end of one optical fiber 5 can also be formed with a light collection point 401 arranged in a line with a marshalling interval by photolithography, etching or coating, and a single optical fiber in-line grating 43 or a single optical fiber staggered grating 44 can also be formed, and the maximum dimension P of the light collection point is within a range of 0.5 to 1.5 times the maximum dimension d of the detection object.

[0026] The second embodiment of the scan recognition system of the present application includes a moving device 1, a detection plate 2, an excitation light source 3, a marshalling in-line grating 4, an optical fiber 5, and a recognition device 6, as shown in FIGS.

[0027] In order to allow the light collection points 401 to be aligned with the detection target 201, reduce interference caused by misalignment between the light collection points 401 and the detection target 201, and improve the accuracy of scan recognition, the detection target 201 is arranged in advance on the detection plate 2 or the test band according to a matrix arrangement. The number of light collection points 401 on the marshalling in-line grating 4 is equal to or greater than the number of columns of the matrix of the detection target 201 on the detection plate 2, so that each detection target 201 in each row corresponds to one light collection point 401. The maximum dimension of the light collection point 401 is within a range of 0.5 to 1.5 times the maximum dimension d of the detection target, so that the size of the light collection point 401 corresponds to the size of the detection target 201. When the light collecting point 401 is aligned close to the detection target 201, it ensures that the optical signal collected by the light collecting point 401 is the optical signal emitted by the aligned detection target 201, avoids the interference of the optical signal emitted by the surrounding detection target 201, and improves the accuracy of scan recognition and detection accuracy. Therefore, the marshalling in-line grating 4 is not elongated, but is square, circular or elliptical, thereby preventing the light emitted by the surrounding detection target 201 of the detection target 201 aligned with the light collecting point 401 from entering the light collecting point 401 and impairing the detection accuracy.

[0028] As shown in FIG. 5, the detection plate 2 or the test band has detection objects 201 arranged on the detection plate 2 in a matrix form, and the matrix spacing of the detection objects is K. The detection objects 201 include molecules of various detection and analysis target substances such as chemical analysis, protein analysis, nucleic acid analysis, cell analysis, exosome analysis, circulating tumor cell analysis, and nanomaterial analysis, and can be applied in the fields of precision medicine, forensic identification, food safety, and environmental protection. A fluorescent marker is bound to some of the detection objects 201, and when the light emitted by the excitation light source 3 is irradiated onto the detection objects 201, the fluorescent marker of the detection objects 201 is excited to emit fluorescence.

[0029] As shown in FIG. 6, the single optical fiber in-line grating of the marshalling in-line grating 4 of the first embodiment uses a single optical fiber in-line grating 43, and forms light collection points 401 arranged linearly at intervals along the grating direction X at one end of an optical fiber 5 by photolithography, etching or coating, and the interval L between adjacent light collection points is the same as the matrix interval K of the object to be detected or √2 times the matrix interval K of the object to be detected.

[0030] As shown in FIG. 7, in the single optical fiber staggered grating of the second embodiment of the marshalling in-line grating, the marshalling in-line grating 4 uses a single optical fiber staggered grating 44 to form light collection points 401 arranged in a staggered pattern at intervals along the grating direction X by photolithography, etching or coating at one end of a single optical fiber 5. The interval L between adjacent light collection points is the same as the row and column interval K of the detection object, and the offset distance M between adjacent light collection points is the same as the row and column interval K of the detection object or an integer multiple of the row and column interval K of the detection object. The light collection points 401 of the single optical fiber staggered grating 44 correspond to two rows of detection objects 201 on the detection plate 2.

[0031] As shown in Figures 8 and 9, in the multi-optical fiber in-line grating of the third embodiment of the marshalling in-line grating, the marshalling in-line grating 4 uses a multi-optical fiber in-line grating 41, and one end of the optical fiber 5 is arranged linearly at intervals L along the lattice direction X. The interval L between adjacent light collection points is the same as the matrix interval K of the detection object or is √2 times the matrix interval K of the detection object. The fluorescence from the detection object 201 is collected by one end of the optical fiber 5, transmitted to the other end of the optical fiber 5 through the optical fiber 5, and converted into an electrical signal by the photosensitive tube of the recognition device 6.

[0032] As shown in Fig. 10 and Fig. 11, in the multi-optical fiber staggered grating of the fourth embodiment of the marshalling in-line grating, the marshalling in-line grating 4 uses a multi-optical fiber staggered grating 42, in which one ends of a plurality of optical fibers 5 are arranged at intervals along the grating direction X, and one ends of adjacent optical fibers 5 are arranged in a staggered pattern perpendicular to the grating direction X. The interval L between adjacent light collecting points is the same as the row and column interval K of the detection object, and the shift distance M between adjacent light collecting points is the same as the row and column interval K of the detection object, or is an integer multiple of the row and column interval K of the detection object. The light collecting points 401 of the multi-optical fiber staggered grating 42, which is one end of the optical fiber 5 of the multi-optical fiber staggered grating 42, correspond to two rows of detection objects 201 on the detection plate 2. This staggered arrangement form can increase the distance between adjacent light collecting points 401, thereby reducing or avoiding optical interference between the detection objects 201 adjacent to the detection objects 201 aligned with the light collecting points 401, and improving the detection accuracy.

[0033] As shown in FIG. 12, in the scan recognition process 1 of the scan recognition method of the marshalling in-line grating of the present application, the detection object 201 is diluted in a solution, and then dispersed and arranged in a tile on the detection plate 2. The moving device 1 drives the detection plate 2 to move relative to the marshalling in-line grating 4. At the same time, the light emitted by the excitation light source 3 is irradiated onto the detection plate 2 or the test band, and a part of the detection object 201 to which the fluorescent marker is bound is excited to emit light. The light collecting point 401 of the multi-fiber in-line grating 41 or the single-fiber in-line grating 43 scans the detection object 201 arranged in a tile on the detection plate 2, and the light collecting point 401 collects the optical signal emitted by the detection object 201 and transmits it to the recognition device 6 via the optical fiber 5, and the photosensitive tube of the recognition device 6 converts the optical signal collected by the marshalling in-line grating 4 into an electrical signal for counting.

[0034] As shown in FIG. 13, in the scan recognition process 2 of the scan recognition method of the marshalling in-line grating of the present application, the moving device 1 drives the detection plate 2 to move it relative to the marshalling in-line grating 4. The light collection points 401 of the multi-fiber in-line grating 41 or the single-fiber in-line grating 43 correspond to the positions of the detection objects 201 distributed in a matrix on the detection plate 2. At the same time, the light emitted by the excitation light source 3 is irradiated onto the detection plate 2, and some of the detection objects 201 to which the fluorescent markers are bound are excited to emit light. When the light collection point 401 is aligned with the detection objects 201 in the first row, it collects the light signals emitted by the emitting detection objects 201 in the first row and transmits them to the recognition device 6 via the optical fiber 5, and the photosensitive tube of the recognition device 6 converts the light signals collected by the marshalling in-line grating 4 into electrical signals for counting. During the relative movement process between the detection plate 2 and the marshalling in-line grating 4, the light collection points 401 of the multi-optical fiber in-line grating 41 or single optical fiber in-line grating 43 sequentially collect the optical signals emitted by the detection objects 201 in each row on the detection plate 2, and calculate the number of emitting detection objects 201 on the detection plate 2, thereby realizing dynamic and continuous counting of the detection objects 201, thereby improving the detection accuracy, shortening the recognition time, and improving the detection efficiency.

[0035] As shown in Fig. 14, in the scan recognition process 3 of the scan recognition method of the marshalling in-line grating of the present application, the grating direction X and the moving direction Y of the detection plate form an oblique angle of 45°. At this time, the interval L between adjacent light collecting points is √2 × the matrix interval K of the detection object, so that each light collecting point 401 of the multi-fiber in-line grating 41 or the single-fiber in-line grating 43 corresponds to each detection object 201 in the 45° direction in the matrix of the detection objects 201 on the detection plate 2. During the relative movement process between the detection plate 2 and the marshalling in-line grating 4, the light collecting point 401 of the multi-fiber in-line grating 41 or the single-fiber in-line grating 43 sequentially collects the optical signals emitted by the detection objects 201 of each row on the detection plate 2, and calculates the number of the light-emitting detection objects 201 on the detection plate 2, thereby realizing the dynamic and continuous counting of the detection objects 201. This detection method can increase the distance between adjacent light collection points 401, reduce or avoid optical interference between the detection object 201 adjacent to the detection object 201 aligned with the light collection point 401, and improve detection accuracy.

[0036] As shown in FIG. 15, in the scan recognition process 4 of the marshalling in-line grating scan recognition method of the present application, the marshalling in-line grating 4 uses a multi-optical fiber staggered grating 42 or a single optical fiber staggered grating 44. The light collection points 401 of the multi-optical fiber staggered grating 42 or the single optical fiber staggered grating 44 correspond to the positions of the two rows of detection objects 201 distributed in a matrix on the detection plate 2. When the front light collection point 401 is skipped and aligned with half of the detection objects 201 in the first row, it collects the optical signal in half of the detection objects 201 in the first row and transmits it to the recognition device 6 via the optical fiber 5, and when the front light collection point 401 is aligned with half of the detection objects 201 in the second row, the rear light collection point 401 is aligned with the remaining half of the detection objects 201 in the first row that are not scanned. During the relative movement process between the detection plate 2 and the marshalling in-line grating 4, the light collecting points 401 of the multi-fiber staggered grating 42 or the single-fiber staggered grating 44 can sequentially collect the optical signals emitted by the detection objects 201 in each row on the detection plate 2, and count the number of emitting detection objects 201 on the detection plate 2, thereby realizing dynamic and continuous counting of the detection objects 201. The light collecting points 401 of the multi-fiber staggered grating 42 or the single-fiber staggered grating 44 correspond to the detection objects 201 in two adjacent rows in the matrix on the detection plate 2, thereby increasing the distance between the adjacent light collecting points 401, reducing or avoiding the optical interference of the detection objects 201 adjacent to the detection objects 201 aligned with the light collecting points 401, and improving the detection accuracy.

[0037] As described above, the beneficial effects of the present invention are that the detection object that emits light can be dynamically and continuously counted using the marshalling in-line grating, thereby shortening the recognition time, improving the detection efficiency, and improving the detection accuracy. In addition, the marshalling in-line grating can be manufactured using optical fiber, which not only simplifies the structure of the scan recognition system, but also reduces the cost.

[0038] The above embodiments are only used to explain the present invention, and are not intended to limit the present invention. Those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions should also fall within the scope of protection of the present invention, and the scope of protection of the present invention should be defined in the claims. [Explanation of symbols]

[0039] 1. Mobile Device 2 Detection plate 3. Excitation Light Source 4 Marshalling Inline Grating 41 Multi-optical fiber in-line grating 42 Multi-optical fiber staggered grating 43 Single Optical Fiber In-line Grating 44 Single Optical Fiber Staggered Grating 401 Light Collection Points 5. Optical Fiber 6 recognition device d Maximum size of object to be detected K: Row and column spacing of detected objects L Spacing between adjacent light collection points M offset distance between adjacent light collection points P is the maximum dimension of the light collecting point Q Fiber Diameter X grid direction Y Detection plate movement direction

Claims

1. A marshalling in-line grating (4) is configured by arranging a plurality of light collecting points (401) in a line at marshalling intervals, and the light collecting points (401) include light transmitting points or reflecting points. A scan recognition method based on the marshalling in-line grating, A scan recognition method based on a marshalling in-line grating, characterized in that a detection object (201) is arranged in a tiled pattern on a detection plate (2), the detection plate (2) is scanned by the relative movement formed by the marshalling in-line grating (4) and the detection plate (1), and the light emitted or reflected by the detection object (201) is collected by a light collection point (401) and transmitted to a recognition device (6) for photoelectric conversion, thereby realizing scan recognition of the detection plate (1).

2. The scan recognition method based on marshalling in-line gratings as claimed in claim 1, characterized in that the detection objects (201) are tiled on the detection plate (1) according to a matrix distribution form, and the spacing (L) between adjacent light collection points of the marshalling in-line grating (4) is the same as the matrix spacing (K) of the detection objects.

3. The scan recognition method based on a marshalling in-line grating according to claim 2, characterized in that the plurality of light collecting points (401) are arranged at intervals along the grating direction (X) to form a marshalling in-line grating (4).

4. The scan recognition method based on marshalling in-line grating according to claim 3, characterized in that adjacent light collecting points (401) are arranged in a staggered manner perpendicular to the grating direction (X).

5. The scan recognition method based on marshalling in-line grating as claimed in claim 4, characterized in that the offset distance (M) of adjacent light collecting points is equal to the interval (L) of adjacent light collecting points.

6. A scan recognition method based on a marshalling in-line grating according to any one of claims 1 to 5, characterized in that the relative movement direction (Y) between the marshalling in-line grating (4) and the detection plate (2) is perpendicular to the grating direction (X).

7. The scanning recognition method based on a marshalling in-line grating as described in claim 6, characterized in that the light emitted or reflected by the detection object (201) is collected by the marshalling in-line grating (4) and then transmitted to the recognition device (6) via an optical fiber (5), the marshalling in-line grating (4) being installed at one end of the optical fiber (5), and the recognition device (6) being installed at the other end of the optical fiber (5).

8. The scan recognition method based on marshalling in-line gratings according to claim 7, characterized in that one end of a plurality of optical fibers (5) is arranged at intervals along the grating direction (X) to form spaced linearly arranged light collecting points (401) to form a multi-optical fiber in-line grating (41) or a multi-optical fiber staggered grating (42).

9. The scan recognition method based on marshalling in-line gratings according to claim 7, characterized in that light collecting points (401) arranged in a line at marshalling intervals are formed on an end face of one end of a single optical fiber (5) by photolithography, etching or coating, to form a single optical fiber in-line grating (43) or a single optical fiber staggered grating (44).

10. A scan recognition system for implementing the scan recognition method according to any one of claims 1 to 9, comprising a detection plate (2) and an excitation light source (3), the excitation light source (3) irradiating the detection plate (2), A scan recognition system for realizing the scan recognition method described in any one of claims 1 to 9, further comprising a marshalling in-line grating (4), an optical fiber (5), a moving device (1), and a recognition device (6), wherein the marshalling in-line grating (4) is installed at one end of the optical fiber (5), and the recognition device (6) is installed at the other end of the optical fiber (5), the moving device (1) drives the detection plate (2) or the marshalling in-line grating (4) to move, thereby forming a relative movement between the marshalling in-line grating (4) and the detection plate (2), the marshalling in-line grating (4) scans the detection plate (2), and the optical signal acquired by the scan is transmitted to the recognition device (6) via the optical fiber (5), and the recognition device (6) converts the optical signal into an electrical signal, thereby realizing scan recognition of the detection plate (2).

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