Skin-improving cosmetic composition containing bakuchiol

Abstract

The present invention relates to a cosmetic composition and a quasi-drug composition containing bakuchiol, milk thistle fruit extract, and cinnamon fruit extract; a skin improvement method; and use thereof. The bakuchiol, milk thistle fruit extract, and cinnamon fruit extract of the present invention have excellent skin-improving effects and are therefore highly useful as cosmetic compositions and quasi-drug compositions for improving skin, which can be expected to be used in a variety of industrial applications, such as cosmetics and quasi-drugs.

Description

[Technical Field]

[0001] The present invention relates to a cosmetic composition and a quasi-drug composition containing bakuchiol, milk thistle fruit extract, and cinnamon fruit extract; a skin improvement method; and use thereof. [Background technology]

[0002] Skin aging, including wrinkles, pigmentation, and dryness, is a term used to describe skin aging. Skin aging can be divided into two categories: chronological aging and extrinsic aging caused by external factors such as ultraviolet A (UV-A), ultraviolet B (UV-B), and air pollution. UV-A, a long-wavelength UV ray (315-400 nm), penetrates deeper into the skin than UV-B, damaging the dermis. UV-B, with its intermediate wavelength (280-315 nm), is known to directly affect DNA and damage the epidermis. UV-B, with its higher energy content than UV-A, is considered the primary cause of disruption of skin homeostasis. UV-B promotes the expression of matrix metalloproteinase-1 (MMP-1), a key collagen-degrading factor that breaks down type I collagen, reducing skin tension and accelerating wrinkle formation.

[0003] In addition, external factors such as cigarette smoke and ozone damage the skin by generating reactive oxygen species (ROS) from skin cells. One consequence of the generation of reactive oxygen species is increased levels of Bcl-2-associated protein-x (Bax), which activates charge-dependent anion channels in the cell membrane and reduces mitochondrial membrane potential. Furthermore, Bax induces the release of cytochrome c from mitochondria, activating caspase-3 and leading to cell death.

[0004] Cosmetic ingredients are generally known to cause various skin problems such as inflammation, rash, and edema (Maibach, HI, Contact Dermatitis, 6, 369-404, 1980). In addition, many substances used as raw materials for functional cosmetics have the problem of low stability or only a slight effect on the skin.

[0005] For example, adenosine, animal placenta-derived proteins, and chlorella extract are known as cosmetics for improving skin wrinkles and elasticity. The most widely known chlorella extract has only a slight effect, and it is difficult to substantially expect the effects of improving skin elasticity and wrinkles. [Prior art documents] [Non-patent literature]

[0006] [Non-Patent Document 1] Maibach.HI, Contact Dermatitis, 6.369-404, 1980 [Non-patent document 2] British Journal of Dermatology (2019) 180,pp289-296 Summary of the Invention [Problem to be solved by the invention]

[0007] The inventors have made extensive efforts to find substances that have excellent skin-improving effects, and as a result have confirmed that bakuchiol, milk thistle fruit extract, and cinnamon fruit extract have skin-improving effects and that their skin-improving effects are significantly superior to those of conventional bakuchiol, thereby completing the present invention. [Means for solving the problem]

[0008] One object of the present invention is to provide a cosmetic composition comprising bakuchiol, milk thistle fruit extract and cinnamon fruit extract.

[0009] Another object of the present invention is to provide a quasi-drug composition comprising bakuchiol, milk thistle fruit extract and cinnamon fruit extract.

[0010] Another object of the present invention is to provide a method for improving skin, comprising applying or administering to the skin of an individual a composition comprising bakuchiol, milk thistle fruit extract, and niacin fruit extract.

[0011] Another object of the present invention is to provide a use of a composition containing bakuchiol, milk thistle fruit extract and cinnamon fruit extract for improving skin. [Effects of the Invention]

[0012] The bakuchiol, milk thistle fruit extract, and cinnamon fruit extract of the present invention have excellent skin-improving effects and are therefore highly useful as cosmetic compositions and quasi-drug compositions for improving skin, and are therefore expected to be used in a variety of industrial applications, such as cosmetics and quasi-drugs. [Brief explanation of the drawings]

[0013] [Figure 1] 1 is a graph showing RARG gene activity of milk thistle fruit extract. [Figure 2] 1 is a graph showing the RAR activity of bakuchiol and milk thistle fruit extract. [Figure 3] 1 is a graph showing the results of human application evaluation of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil. [Figure 4] These are photographs showing the results of human application evaluation of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil. [Figure 5] These are 3D skin cross-section results showing the photoaging improving effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil. [Figure 6]1 is a graph showing the photoaging improving effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil. [Figure 7] 1 is a graph showing the effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil on improving skin irritation and inflammation. DETAILED DESCRIPTION OF THE INVENTION

[0014] This will be explained in more detail as follows. Meanwhile, each description and embodiment disclosed in the present invention also applies to each other description and embodiment. That is, any combination of various elements disclosed in the present application falls within the scope of the present invention. Furthermore, the following specific description is not considered to limit the category of the present application.

[0015] Additionally, those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein and such equivalents are intended to be encompassed by the present invention.

[0016] One aspect of the present invention provides a cosmetic composition comprising bakuchiol, milk thistle fruit extract, and niacin fruit extract.

[0017] The term "bakuchiol" used in this invention refers to a natural plant-derived ingredient and a meroterpene of the terpenophenol series. It is extracted from the seeds of the Dutch ibis and is known to have antioxidant activity. Bakuchiol has a similar gene expression pattern to retinoids in the skin, and has begun to be referred to as a natural plant retinol. However, the two substances have no structural similarity and differ in their mechanisms of action. Bakuchiol is known to have lower expression and activity of retinoic acid receptors induced by retinol, resulting in inferior wrinkle improvement efficacy compared to retinol.

[0018] The bakuchiol of the present invention may have the following structure:

[0019] [ka]

[0020] The present invention is not particularly limited to a method for obtaining bakuchiol, and it may be collected from nature, chemically synthesized by a method known in the art, or commercially available.

[0021] Furthermore, the bakuchiol includes all derivatives of bakuchiol that can be expected by a person skilled in the art, and is included without limitation as long as it has the same effect in the present invention.

[0022] The term "Silybum Marianum (Sm)" as used herein refers to a plant of the Asteraceae family, also known as milk thistle. Milk thistle extracts have traditionally been known to be effective against various liver diseases, such as hepatitis and alcoholic liver disease, and have been used in traditional medicine for centuries.

[0023] The term "Terminalia Chebula (medicinal terminalia fruit)" as used herein refers to the dried mature fruit of Terminalia chebula or Terminalia chebula, a deciduous tree belonging to the family Combretaceae. Terminalia chebula is also known as Terminalia chebula, Terminalia chebula, Terminalia sieboldii, and Terminalia pithii, and is used for medicinal purposes in Asia. In traditional Chinese medicine, Terminalia chebula has a bitter taste and warm medicinal properties, and is widely used for astringency, hemostasis, and the treatment of chronic laryngitis.

[0024] The plant may refer to specific parts of milk thistle or milk thistle such as the root, leaves, flowers, stems, fruits, etc., or may refer to the entire plant. In a specific embodiment, the composition of the present invention includes an extract of milk thistle or milk thistle fruit.

[0025] The term "extract" as used herein refers to a liquid component obtained by immersing a target substance in various solvents and then extracting it at room temperature or at elevated temperatures for a certain period of time, or a solid component obtained by removing the solvent from the liquid component. The extraction may be performed one or more times, but is not limited thereto. Furthermore, the term "extract" may be interpreted as encompassing all of the resulting products, including filtrates, dilutions, concentrates thereof, and crude, purified, and dried products thereof. Therefore, the term "milk thistle fruit extract" and "sweet almond fruit extract" provided herein may be interpreted as including the extract itself and all formulations of the extract that can be prepared using the extract, such as an extract obtained by extracting milk thistle fruit or milk almond fruit, a purified product obtained by enzymatic hydrolysis of the extract, or a mixture thereof, and / or a dried product thereof.

[0026] The method for extracting the milk thistle fruit extract and milk thistle fruit extract of the present invention is not particularly limited, and can be any method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which can be performed alone or in combination of two or more methods.

[0027] Examples of the solvent used in producing the extract include water, alcohol, and a mixture of these solvents, which may be used alone or in combination of two or more. When alcohol is used as the solvent, specifically, an alcohol having 1 to 4 carbon atoms, ethanol, methanol, or butanol can be used.

[0028] In the present invention, the bakuchiol, milk thistle fruit extract, and cinnamon bark fruit extract may be mixed in a weight ratio of 1:0.1:0.1 to 1:100:100, specifically 1:1:1 to 1:50:50, more specifically 1:1:1 to 1:10:10.

[0029] In the present invention, "retinoic acid receptor (RAR)" refers to a receptor present in the nucleus that is necessary for the action of retinoic acid, which can be said to be the active form of vitamin A. When retinoic acid is applied to the skin, the number of fibroblasts that synthesize collagen increases, leading to increased synthesis of new collagen. The new collagen produced by fibroblasts accumulates at the site where the epidermis and dermis meet, ultimately regenerating the damaged dermis, improving wrinkles and skin elasticity. Retinoic acid is known to have the effect of regenerating collagen fibers as well as regenerating damaged elastic fibers.

[0030] In the present invention, "retinoic acid receptor gamma (RARG, RARγ)" refers to a retinoid receptor gene, which requires the binding and increased activity of retinoic acid in order for retinol-induced responses such as wrinkle improvement to occur.

[0031] In the present invention, "ITGA6 (Integrin alpha 6)" is a gene known to play a role in strengthening the skin barrier and plays an anti-aging role by directly binding to laminin, which connects and fixes the dermis and epidermis in the basement membrane between the dermis and epidermis, providing stability and maintaining the skin structure. In the present invention, "strengthening the skin barrier" refers to all actions that enhance the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients.

[0032] In one example of the present invention, it was confirmed that RARG gene expression increased when treated with milk thistle fruit extract (Figure 1), and that RAR activity increased when treated with both bakuchiol and milk thistle fruit extract compared to treatment with bakuchiol alone (Figure 2).

[0033] In another example of the present invention, it was confirmed that when bakuchiol and Psammia chinensis fruit extract were treated together, ITGA6 gene expression increased compared to when bakuchiol was treated alone (Table 2).

[0034] In the present invention, the cosmetic composition may further contain mastic gum or rosehip oil, but is not limited thereto.

[0035] The term "mastic gum" used in this invention is made by drying the sap of the mastic tree, which has been known to have been used as a medicine for stomach health since ancient times in Greece.

[0036] The term "rosehip oil" as used herein refers to oil extracted from the fruits and seeds of the dog rose (Rosa canina). Rosehip oil is known to improve moisturizing by blocking moisture loss.

[0037] In the present invention, the mastic gum may be mixed in an amount of 0.0001 to 100, specifically 0.001 to 50, more specifically 0.01 to 10% by weight based on 1% by weight of bakuchiol, and the rosehip oil may be mixed in an amount of 0.001 to 100, specifically 0.01 to 10, more specifically 0.1 to 5% by weight based on 1% by weight of bakuchiol.

[0038] In one example of the present invention, it was confirmed that when bakuchiol and rhododendron fruit extract were further mixed with mastic gum and / or rosehip oil, the expression level of ITGA6 gene increased compared to the bakuchiol and rhododendron fruit extract treatment (Table 2).

[0039] In the present invention, the composition may have a skin improving effect.

[0040] The term "skin improvement" in the present invention may refer to any one or more selected from the group consisting of wrinkle improvement, elasticity improvement, aging improvement, skin irritation improvement, and inflammation improvement, but is not limited thereto.

[0041] The term "improvement" as used herein means any action that at least reduces a parameter associated with the alleviation or treatment of a condition, for example, the severity of a symptom, and may include any one or more of wrinkle improvement, elasticity improvement, aging improvement, skin irritation improvement, and inflammation improvement.

[0042] The skin improvement may be achieved through, but is not limited to, activating retinoic acid receptors, increasing retinoic acid receptor gamma gene expression, increasing ITGA6 gene expression, promoting collagen synthesis, or reducing the expression of inflammatory substances.

[0043] Specifically, the inventors have confirmed that compositions containing bakuchiol, milk thistle fruit extract, and cinnamon fruit extract, or a mixture thereof further containing mastic gum and / or rosehip oil, have excellent skin-improving effects. More specifically, compositions containing bakuchiol, milk thistle fruit extract, and cinnamon fruit extract, or a mixture thereof further containing mastic gum and / or rosehip oil, have excellent effects such as activating retinoic acid receptors, increasing retinoic acid receptor gamma gene expression, increasing ITGA6 gene expression, promoting collagen synthesis, and reducing the expression of inflammatory substances. Therefore, compositions containing bakuchiol, milk thistle fruit extract, and cinnamon fruit extract, or a mixture thereof further containing mastic gum and / or rosehip oil, have excellent skin-improving effects such as wrinkle reduction, elasticity improvement, anti-aging, skin irritation reduction, and inflammation reduction.

[0044] The term "wrinkles" as used herein can be broadly divided into expression wrinkles caused by facial muscles used to create facial expressions and fine wrinkles caused by generalized or localized skin aging. Specifically, wrinkles are caused by genetic factors, a decrease in collagen and elastic fibers present in the dermis of the skin, external environment, etc. For purposes of the present invention, the term "wrinkles" may be used to include both expression wrinkles and fine wrinkles, but is not limited thereto.

[0045] As used herein, the term "elasticity" refers to the force of tension when the skin is pressed with a finger, and "improving elasticity" or "enhancing elasticity" refers to enhancing the elasticity of the skin by strengthening the structure of the subcutaneous fat layer of an individual, and refers to enhancing the elasticity of the skin by using the composition of the present invention.

[0046] The term "collagen" as used herein refers to a fibrous protein found in abundance in many animals, particularly mammals, and accounts for the majority of all connective tissues in the body, including skin and cartilage. Collagen is produced and secreted by fibroblasts, the most common cells in the body. Gelatin, which is widely used in cooking and in the food and pharmaceutical industries, is produced by irreversible hydrolysis of collagen. Collagen loss is the main cause of wrinkles and reduced skin elasticity, so collagen synthesis is essential for wrinkle improvement and elasticity improvement.

[0047] In one example of the present invention, treatment with bakuchiol, milk thistle extract, and cornstarch fruit extract significantly increased collagen synthesis compared to treatment with bakuchiol alone. Furthermore, the addition of mastic gum and / or rosehip oil to bakuchiol, milk thistle extract, and cornstarch fruit extract further increased collagen synthesis (Table 3).

[0048] In another example of the present invention, a formulation containing bakuchiol, milk thistle fruit extract, citrus fruit extract, mastic gum, and rosehip oil was administered to a human body, and it was confirmed that it improved wrinkles under the eyes, nasolabial folds, and cheek elasticity (Figures 3 and 4).

[0049] The term "aging" as used herein refers to the phenomenon of deterioration of biological structures and functions over time, and the anti-aging of the present invention is not limited to the degree of improvement, as long as it prevents, inhibits, delays, or otherwise improves aging. In the present invention, wrinkle improvement and anti-aging may be achieved by promoting collagen synthesis, but are not limited thereto.

[0050] Photoaging, the main cause of extrinsic aging, is caused by UV rays, which can be divided into UVA, UVB, and UVC depending on their wavelength. UVB (ultraviolet-B) has the greatest impact on the skin, penetrating primarily into the epidermis and affecting skin cells. UVA (ultraviolet-A) penetrates deep into the dermis and primarily affects skin cells involved in collagen synthesis. Wrinkle formation, a typical phenomenon of skin aging, is primarily caused by a decrease in collagen in dermal tissue. Dermal fibroblasts, which make up the dermis, regulate the expression of collagen, elastin, and MMPs. UV exposure reduces the synthesis of structural proteins such as collagen and elastin and increases the expression of MMPs.

[0051] The term "photoaging" as used herein refers to a phenomenon in which skin elasticity and moisture are reduced and wrinkles are formed due to repeated or prolonged exposure to ultraviolet rays from sunlight, resulting in skin damage.

[0052] In one example of the present invention, it was confirmed that treatment with bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil significantly improved the skin barrier strengthening effect, prevented collagen degradation, and provided cell protection against UV rays, thereby significantly improving the photoaging effect (Figures 5 and 6).

[0053] The term "improvement of skin irritation" as used herein means alleviating and soothing erythema, itching, or irritated skin sites, and may be a concept that includes alleviating skin irritation. Examples include, but are not limited to, alleviation of skin pruritus, alleviation of skin pain, reduction of skin moisture loss, and / or reduction of redness.

[0054] The term "inflammation" as used herein refers to a type of in vivo response to specific tissue damage or infection, with immune cells being the main mediators. The purpose of such inflammation is to minimize tissue damage, remove infectious agents, and achieve tissue regeneration. In the present invention, the improvement, soothing, and regeneration of skin irritation may be achieved by, but is not limited to, suppression of inflammatory substances (IL-1a).

[0055] Retinol and bakuchiol are known to be inflammatory factor stimulators, and have been known to cause problems such as redness, irritation, and inflammation (British Journal of Dermatology (2019) 180, pp. 289-296).

[0056] In contrast, in one example of the present invention, when bakuchiol was applied alone, irritation did not decrease but rather increased. In contrast, when bakuchiol, milk thistle fruit extract, saffron fruit extract, mastic gum, and rosehip oil were applied, the expression of the inflammatory factor IL-1a induced by irritants was reduced (Figure 7).

[0057] The term "cosmetic composition" as used herein may be in the form of a formulation selected from the group consisting of, but is not limited to, a solution, an ointment for external use, a cream, a foam, a nourishing lotion, a softening lotion, a perfume, a pack, a softening lotion, a milky lotion, a makeup base, an essence, a soap, a liquid cleanser, a bath additive, a sunscreen cream, a sun oil, a suspension, an emulsion, a paste, a gel, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, a patch, and a spray.

[0058] The cosmetic composition of the present invention may further contain one or more cosmetically acceptable carriers that are commonly incorporated into skin cosmetics, and may contain, as appropriate, common ingredients such as oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, and fragrances, but is not limited thereto.

[0059] The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

[0060] When the cosmetic formulation of the present invention is an ointment, paste, cream, or gel, the carrier component may be, but is not limited to, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. These may be used alone or in combination of two or more.

[0061] When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, etc., and particularly, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil, sesame oil, glycerol aliphatic esters, polyethylene glycol, or sorbitan fatty acid esters, but are not limited thereto. These may be used alone or in combination of two or more.

[0062] When the dosage form of the present invention is a suspension, the carrier component may be, but is not limited to, a liquid diluent such as water, ethanol, or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tragacanth, which may be used alone or in combination.

[0063] When the dosage form of the present invention is a soap, the carrier component may be, but is not limited to, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. These may be used alone or in combination of two or more.

[0064] When the dosage form of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and particularly when it is a spray, it may further contain a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether.

[0065] Meanwhile, all of the components described in the present invention may be included in the composition of the present invention within a range that does not exceed the maximum use values ​​specified in regulations regarding cosmetic safety standards and the Chinese "Cosmetic Safety and Technical Standards."

[0066] Another aspect of the present invention provides a quasi-drug composition comprising bakuchiol, milk thistle fruit extract, and niacin fruit extract.

[0067] The "bakuchiol," "milk thistle fruit extract," and "calendula fruit extract" are as described above.

[0068] In the present invention, the composition may have a skin improving effect.

[0069] The "skin improvement" is as described above.

[0070] In the present invention, the quasi-drug composition may further contain mastic gum or rosehip oil, but is not limited thereto.

[0071] The "mastic gum" and "rosehip oil" are as described above.

[0072] The term "quasi-drug" as used herein refers to an article that has a milder effect than a drug among articles used for the purpose of diagnosing, curing, improving, alleviating, treating, or preventing a disease in humans or animals. For example, quasi-drugs under the Pharmaceutical Affairs Law exclude articles used for pharmaceutical purposes, and include products used to treat or prevent diseases in humans / animals, and products that have a mild effect on the human body or do not have a direct effect.

[0073] Specifically, the quasi-drug may include an external skin preparation and a personal hygiene product, and more specifically, may be, but is not limited to, a disinfectant, a shower foam, a mouthwash, a wet tissue, a detergent soap, a hand wash, or an ointment.

[0074] When the composition according to the present invention is used as a quasi-drug additive, the composition may be added as it is or may be used together with other quasi-drugs or quasi-drug ingredients, or may be used appropriately in a conventional manner. The amount of the active ingredient to be mixed may be determined appropriately depending on the purpose of use.

[0075] Another aspect of the present invention provides a method for improving skin, comprising applying or administering to the skin of an individual a composition comprising bakuchiol, milk thistle fruit extract, and niacin fruit extract.

[0076] The composition may further comprise, but is not limited to, mastic gum or rosehip oil.

[0077] The composition may be, but is not limited to, a cosmetic or quasi-drug composition.

[0078] The "bakuchiol," "milk thistle fruit extract," "calendula fruit extract," "cosmetic composition," "mastic gum," and "rosehip oil" are as described above.

[0079] The term "individual" as used herein refers to any animal, including humans, that needs improvement of skin wrinkles, elasticity, aging, irritation, or inflammation, or that has collagen deficiency or skin that is prone to erythema, itching, or irritation. The animal may be not only a human, but also a mammal such as a cow, horse, sheep, pig, goat, camel, antelope, dog, or cat that needs treatment for symptoms similar to those of humans, but is not limited thereto.

[0080] In the present invention, the term "application" means any method of contacting the composition according to the present invention with the skin of an individual in any suitable manner, with the aim of allowing the composition to be absorbed into the skin.

[0081] When the composition of the present invention is applied to the skin of an individual, it has the effects of improving wrinkles, elasticity, aging, skin irritation, and inflammation, and may include a step of applying or administering the composition to an individual in an amount effective to exhibit the effects.

[0082] In the present invention, the term "administration" is a general term for providing a predetermined substance to an individual by any appropriate method, and the administration route may be any common route as long as it can reach the target skin.

[0083] Another aspect of the present invention provides use of a composition comprising bakuchiol, milk thistle fruit extract and niacin fruit extract for improving skin.

[0084] The composition may further comprise, but is not limited to, mastic gum or rosehip oil.

[0085] The composition may be, but is not limited to, a cosmetic or quasi-drug composition.

[0086] The "bakuchiol," "milk thistle fruit extract," "calendula fruit extract," "cosmetic composition," "skin improvement," "mastic gum," and "rosehip oil" are as described above. [Example]

[0087] The present invention will be described in more detail below through examples and experimental examples. However, these examples and experimental examples are for illustrative purposes only and the scope of the present invention is not limited to these examples and experimental examples.

[0088] Experimental Example 1. Sample Preparation The bakuchiol used in this study was Sytenol A (raw material) from Sytheon, milk thistle fruit extract was Silymarin phytosome (raw material) from Indena, saffron fruit extract was Synastol TC (raw material) from Sytheon, rosehip oil was ROSEHIP OIL REFINED (raw material) from TEXTRON TECNICA, and mastic gum was Novoretin (raw material) from Mibelle.

[0089] Example 1. Evaluation of Retinoic Acid Receptor Gamma (RARG) and Retinoic Acid Receptor (RAR) Gene Activity of Bakuchiol and Milk Thistle Fruit Extract Example 1-1. Evaluation of RARG gene activity of milk thistle fruit extract The milk thistle fruit extract (Silybum Marianum Fruit Extract) used contained 17.9% silymarin as an index component. To measure the expression level of RARG, a retinoic acid receptor gene in the cell nucleus, human dermal fibroblasts were placed in a 6-well plate at 5 × 10 5 The cells were dispensed into each well and cultured overnight in a CO2 incubator. Substances were added to the culture medium at different concentrations and then cultured for 24 hours. RNA was extracted using the RNA-spin Total RNA Extraction Kit (Intron Biotechnology, Korea) according to the protocol, and real-time qPCR was performed using a Taqman probe to compare and analyze the RARG gene expression levels of milk thistle fruit extracts, and the measurement results were confirmed (Figure 1).

[0090] As a result, it was confirmed that RARG gene expression increased when treated with milk thistle fruit extract.

[0091] Example 1-2. Evaluation of RAR activity of bakuchiol and milk thistle fruit extract Bakuchiol (BK) and milk thistle fruit extract (SM) were mixed with DMEM media (Dulbecco Modified Eagle Medium) at the concentrations shown in Table 1 below, and then applied to cells. Specifically, to measure the RAR activity caused by retinoic acid converted from retinol entering the cell nucleus and binding to the retinoic acid receptor (RAR), the RARγReporter (Luc)-HEK293 cell line (BPS bioscience, USA) was purchased and used for the experiment. Cells were plated at 10 4 Cells were dispensed into each well and cultured overnight in a CO2 incubator. Bakuchiol (BK) and milk thistle fruit extract (SM) were added to the culture medium at different concentrations, and the cells were cultured for 24 hours. After the culture was completed, differences in retinoic acid receptor activity were compared using luciferase analysis with the Steady-Glo® Luciferase Assay System (Promega, USA) (Figure 2).

[0092] [Table 1]

[0093] As a result, it was found that when 10 ppm of milk thistle fruit extract and bakuchiol were treated together, RAR activity was promoted by more than 1.6 times compared to the control group, confirming that RAR activity was increased more than when milk thistle fruit extract was treated alone.

[0094] This confirmed that milk thistle fruit extract increased RAR activity compared to bakuchiol alone, demonstrating that milk thistle fruit extract is an excellent material for increasing RAR activity.

[0095] Example 2. Evaluation of ITGA6 gene expression levels in bakuchiol, citrus fruit extract, mastic gum, and rosehip oil Bakuchiol, citrus fruit extract, mastic gum, and rosehip oil were mixed in DMEM media at the concentrations shown in Table 2 below and then applied to cells. Specifically, to measure the expression level of ITGA6 gene, which is related to barrier function, keratinocyte cells HaCaT were cultured in 5 x 10 wells on a 6-well plate. 5 The cells were dispensed into each well and cultured overnight in a CO2 incubator. Substances were added to the culture medium at different concentrations and then cultured for 24 hours. RNA was extracted using the RNA-spin Total RNA Extraction Kit (Intron Biotechnology, Korea) according to the protocol. Real-time qPCR was performed using a Taqman probe to compare and analyze ITGA6 gene expression levels, and the measurement results were confirmed (Table 2).

[0096] [Table 2]

[0097] The results showed that ITGA6 gene expression levels were significantly increased when treated with bakuchiol and crocus fruit extract; bakuchiol and mastic gum; bakuchiol, crocus fruit extract and mastic gum; or bakuchiol, crocus fruit extract, mastic gum and rosehip oil, compared to treatment with bakuchiol alone. In particular, when treated with bakuchiol and crocus fruit extract, ITGA6 gene expression was increased due to the synergistic effect of crocus fruit extract. Treatment with bakuchiol, crocus fruit extract, mastic gum and rosehip oil showed the highest ITGA6 gene expression levels.

[0098] This shows that when bakuchiol is combined with rhododendron fruit extract, mastic gum, or rosehip oil, the expression of ITGA6, a bakuchiol-specific gene, increases, indicating that the function of the skin barrier is enhanced.

[0099] Example 3. Confirmation of the wrinkle and elasticity improving effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil Example 3-1. Evaluation of the collagen synthesis enhancement effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil Bakuchiol, milk thistle fruit extract, citrus fruit extract, mastic gum, and rosehip oil were mixed in DMEM media at the concentrations shown in Table 3 below and then applied to cells. Specifically, to evaluate collagen biosynthesis, human dermal fibroblasts were first cultured at 2 x 10 in a 24-well plate. 4 Cells were dispensed into each well and cultured overnight in a CO2 incubator. After removing the culture medium from each well, the wells were gently washed with PBS. Culture medium was then replaced with new medium, and the substances were added at the indicated concentrations and combinations. The culture medium was then recovered, and procollagen secreted from the culture medium was measured using a procollagen type C-peptide EIA kit (MK-101, Takara Bio, Japan).

[0100] [Table 3]

[0101] As a result, it was confirmed that when bakuchiol was mixed with milk thistle fruit extract or milk thistle fruit extract, the amount of collagen synthesis increased slightly compared to when bakuchiol, milk thistle fruit extract, or milk thistle fruit extract was treated alone.

[0102] However, when treated with a mixture of bakuchiol, milk thistle fruit extract, and rosehip oil, collagen synthesis increased by more than 85% compared to untreated control, demonstrating a synergistic effect when bakuchiol is combined with milk thistle fruit extract and rosehip oil.In particular, the addition of mastic gum and / or rosehip oil further increased collagen synthesis.

[0103] As a result, it was found that the bakuchiol, milk thistle fruit extract, and cornstarch fruit extract; bakuchiol, milk thistle fruit extract, cornstarch fruit extract, and mastic gum; bakuchiol, milk thistle fruit extract, cornstarch fruit extract, and rosehip oil; and bakuchiol, milk thistle fruit extract, cornstarch fruit extract, mastic gum, and rosehip oil of the present invention exhibit excellent effects in improving skin wrinkles and increasing elasticity.

[0104] Example 3-2. Evaluation of the human application of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil Four boosters were prepared: 0.1% bakuchiol, milk thistle fruit extract (Silymarin phytosome 0.5%), citrus fruit extract (Synastol TC 0.5%), mastic gum (Novoretin 0.1%), and rosehip oil (Rosehip oil 0.01%). A control cream containing 0.1% bakuchiol was also prepared. These were applied twice daily (once in the morning and once in the evening) for a total of four weeks to the under-eye area, nasolabial folds, and cheeks of 12 external female subjects in their 40s and 50s. Wrinkle improvement rates were measured using Antera 3D for under-eye and nasolabial folds, and Cutometer for cheek elasticity. The average wrinkle improvement rate across all subjects and an image of improvement for one representative subject are shown below (Figures 3 and 4).

[0105] The results showed that treatment with bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil improved under-eye wrinkles by approximately 2.2 times, nasolabial folds by approximately 2.6 times, and cheek elasticity by approximately 8 times compared to treatment with bakuchiol alone.

[0106] In addition, the improvement rate of under-eye wrinkles increased by approximately 5.3 times, and the depth and length of nasolabial folds were reduced by approximately 3.1 times when treated with bakuchiol, milk thistle fruit extract, citrus fruit extract, mastic gum, and rosehip oil.

[0107] As a result, it was found that the bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil of the present invention have excellent effects in improving wrinkles and elasticity.

[0108] Example 4. Confirmation of the effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil on improving photoaging, skin irritation, and skin inflammation Example 4-1. Confirmation of the photoaging improving effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil Tegoscience's Neoderm®-ED, a 3D reconstructed human skin model consisting of epidermis and dermis, was treated with bakuchiol, milk thistle fruit extract, citrus fruit extract, mastic gum, and rosehip oil (four boosters); as a control, bakuchiol and retinol were each treated overnight, followed by UV irradiation at 50 mJ / cm. 2 The cells were cultured while being exposed to UVB twice a day for a total of three days. After the culture was completed, paraffin blocks were prepared and stained with Masson's trichrome to confirm the cross-section of the 3D skin (Figure 5, red: epidermis, blue: dermal collagen). The dermal collagen area was then measured using the publicly available software, Image J (Figure 6).

[0109] As a result, repeated UVB irradiation reduced dermal collagen area by approximately 24% compared to untreated, indicating UV damage to the dermis. Treatment with 0.1% retinol or 0.5% bakuchiol restored collagen area by approximately 15%, demonstrating that bakuchiol is superior to retinol in protecting the epidermal barrier, as shown in Figure 5. However, even when the concentration of bakuchiol was increased to 1%, the dermal collagen recovery effect did not increase any further, and in fact decreased.

[0110] It was confirmed that treatment with bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil of the present invention had excellent epidermal barrier protection effects and significantly improved dermal collagen area recovery ability compared to treatment with retinol or bakuchiol alone.

[0111] As a result, it was found that the bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil of the present invention not only have excellent effects in improving wrinkles and elasticity, but also in improving photoaging.

[0112] Example 4-2. Confirmation of the effects of bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil on improving skin irritation and inflammation The 3D human skin model was cultured while being treated with the extract and UVB in the same manner as in Example 4-1. The culture medium was then collected and the amount of secreted inflammatory substance (IL-1a) was measured using the Human IL-1 alpha / IL-1F1 DuoSet ELISA kit (Figure 7).

[0113] The results showed that IL-1a levels increased when skin cells were stimulated by UV rays, and that treatment with bakuchiol, a known inducer of inflammatory factors, did not reduce the irritation but rather increased it.In contrast, the bakuchiol, milk thistle fruit extract, cinnamon fruit extract, mastic gum, and rosehip oil of the present invention were found to reduce the expression of the inflammatory factor IL-1a induced by irritants.

[0114] This shows that when bakuchiol was used alone, it caused skin irritation and inflammation, but when bakuchiol of the present invention was used in combination with milk thistle fruit extract, citrus fruit extract, mastic gum, and rosehip oil, the expression of inflammatory factors was reduced, resulting in excellent effects in improving skin irritation and inflammation.

[0115] From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are merely illustrative and not limiting. The scope of the present invention should be interpreted as including within the meaning and scope of the claims below, and all modifications and variations derived from the equivalent concepts thereof, rather than the above detailed description.

Claims

1. A cosmetic composition comprising bakuchiol, milk thistle fruit extract and cinnamon fruit extract.

2. The cosmetic composition of claim 1 , further comprising mastic gum or rosehip oil.

3. The cosmetic composition according to claim 1 , which is for improving skin.

4. The cosmetic composition for improving skin according to claim 3, wherein the skin improvement is at least one selected from the group consisting of wrinkle improvement, elasticity improvement, aging improvement, skin irritation improvement, and inflammation improvement.

5. The cosmetic composition according to claim 4, which has the effect of activating retinoic acid receptors, increasing retinoic acid receptor gamma gene expression, increasing ITGA6 gene expression, promoting collagen synthesis, or reducing the expression of inflammatory substances.

6. 2. The cosmetic composition according to claim 1, wherein the bakuchiol, milk thistle fruit extract, and cinnamon bark fruit extract are mixed in a weight ratio of 1:0.1:0.1 to 1:100:

100.

7. A quasi-drug composition comprising bakuchiol, milk thistle fruit extract, and cinnamon fruit extract.

8. The quasi-drug composition according to claim 7, further comprising mastic gum or rosehip oil.

9. A method for improving skin, comprising applying or administering to an individual a composition comprising bakuchiol, milk thistle fruit extract, and niacin fruit extract.

10. The skin improvement method according to claim 9, wherein the composition is a cosmetic or quasi-drug composition.

11. Use of a composition containing bakuchiol, milk thistle fruit extract and cinnamon fruit extract for improving skin.

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