Cultivation medium for Lion's Mane mushroom, cultivation method for Lion's Mane mushroom

Abstract

This provides novel techniques for cultivating Lion's Mane mushrooms. [Solution] A growing medium for cultivating Lion's Mane mushrooms, containing coffee grounds.

Description

Technical Field

[0001] The present invention relates to the cultivation of Hericium erinaceus.

Background Art

[0002] Hericium erinaceus is a kind of mushroom belonging to the family Hericiaceae and is used as a raw material for food, traditional Chinese medicine, health supplements and supplements. As a cultivation method of Hericium erinaceus, for example, the method described in Patent Document 1 has been proposed.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] One of the objects of the present invention is to provide a novel technique for the cultivation of Hericium erinaceus.

Means for Solving the Problems

[0005] The inventor conceived of cultivating Hericium erinaceus using a novel culture medium. As a result of intensive research, the inventor found that Hericium erinaceus can be cultivated in bed cultivation using a culture medium containing coffee grounds.

[0006] The gist of the present invention is as follows. [1] A culture medium for cultivating Hericium erinaceus, containing coffee grounds. [2] A method for cultivating Hericium erinaceus, including cultivating Hericium erinaceus using a culture medium containing coffee grounds.

Effects of the Invention

[0007] According to the present invention, a novel technique for cultivating Lion's Mane mushroom can be provided. [Modes for carrying out the invention]

[0008] One embodiment of the present invention will be described in detail below. This embodiment relates to the cultivation of Lion's Mane mushroom, and involves cultivating Lion's Mane mushroom in a culture medium containing coffee grounds.

[0009] As mentioned above, Hericium erinaceus is a species of mushroom belonging to the family Hericaceae. In this embodiment, specifically, the mycelium (spawn) of Hericium erinaceus is inoculated into a culture medium containing coffee grounds and cultured.

[0010] In this specification, coffee grounds refer to the residue generated after roasted coffee beans have been ground and coffee has been extracted from the resulting powder. There are no particular restrictions on the variety of coffee beans used as the raw material for the coffee grounds in this embodiment; for example, Arabica, Robusta, and Liberica varieties can be used. Furthermore, there are no particular restrictions on the origin of the coffee beans. The raw material for the coffee grounds may be a single variety or single origin coffee bean, or a mixture of multiple varieties or origins.

[0011] There are no particular limitations on the method of extracting coffee from coffee beans, roasting temperature, or roasting environment. For example, roasting methods include direct-fire, hot-air, and semi-hot-air. The degree of roasting of the coffee beans is also not particularly limited, and can include light, cinnamon, medium, high, city, full city, French, and Italian. Furthermore, the type of solvent used for extraction is not particularly limited, but water is one example.

[0012] In this embodiment, the coffee grounds can be used either in their moist state immediately after brewing or after being dried. If dried, the drying method is not particularly limited and can include forced drying using a hot air convection dryer or natural drying in the sun.

[0013] Coffee grounds act in the culture medium, for example, as a culture medium base material. In this embodiment, the content of coffee grounds in the culture medium is not particularly limited and can be, for example, 10% by mass or more and 100% by mass or less, and is preferably 20% by mass or more and 70% by mass or less from the viewpoint of promoting the growth of Lion's Mane mushroom.

[0014] The culture medium according to this embodiment may contain other components in addition to coffee grounds, as long as they can achieve the objectives of the present invention, and is not particularly limited. Other components include, for example, water, components that act as a culture medium, and nutrients. Examples of components that act as culture medium substrates include corn cob meal, sawdust, sawdust, wood chips, and chip dust. Examples of nutritional supplements include corn bran, corn cobs, rice bran, sugars, wheat bran, brewer's meal, dried brewer's yeast, tofu meal, peanut meal, rapeseed meal, cottonseed meal, potato protein, corn gluten feed, soybean meal, soybean meal, protein, minerals, and vitamins.

[0015] The culture medium of this embodiment can be prepared by mixing coffee grounds and other components as needed. There are no particular restrictions on the pH or moisture content of the culture medium; for example, a pH of 4.0 to 7.0 and a moisture content of 40 to 80% would suffice.

[0016] The cultivation of Lion's Mane mushrooms in this embodiment can be carried out by cultivating Lion's Mane mushrooms using the culture medium of this embodiment. Furthermore, other conditions may be known conditions used in the cultivation of Lion's Mane mushrooms on substrates, and are not particularly limited. For example, after performing a sterilization treatment (e.g., high-pressure sterilization treatment at 121°C for 60 minutes in a medium), inoculate the medium with the spawn of Lentinus edodes. Thereafter, culture the mycelium in the dark at 15 to 25°C and a humidity of 50 to 100% for about 0.5 to 1.5 months, and perform cultivation at 15 to 25°C and a humidity of 50 to 100% for about 0.5 to 1.5 months from those in which fruiting bodies are formed in the light. Also, in the stage of culturing the mycelium, treatments such as temperature reduction and removal of bacteria may be performed to promote the formation of fruiting bodies.

[0017] As described above, according to the present embodiment, a novel technique for cultivating Lentinus edodes can be provided.

Example

[0018] Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

[0019] [Preparation of medium containing coffee grounds] 800 g of roasted coffee powder was added to 8000 g of hot water at 95°C. After sufficient water supply for 20 minutes, extraction was performed. After extraction, filtration was performed using a 40-mesh sieve to obtain coffee grounds. The weight of the coffee grounds after filtration was measured, and the moisture content was calculated using the following formula. Moisture content = (weight after filtration (g) - weight of coffee powder (g)) / weight after filtration (g) × 100 (%) Thereafter, water was added to adjust the moisture content to 60% to obtain a medium.

[0020] [Cultivation of Lentinus edodes and other mushrooms] The obtained medium was placed in a polypropylene container (Iwaki wide-mouth bottle (250 ml) manufactured by AS ONE Corporation), and high-pressure sterilization treatment was performed at 121°C for 60 minutes in the medium. After the sterilization treatment, the mycelium of the mushrooms shown in Table 1 was inoculated into the medium, and cultivation was performed under the condition of 23°C. The humidity conditions are as follows. Pholiota nameko: around 90% Shiitake: 70 - 80% Hairy wood ear mushroom: 60-90% Tamogitake mushroom: 85-95% Hatake Shimeji: 90% or more Willow mushroom: 80-90% Pleurotus ostreatus: 70-90% Himalayan oyster mushroom: 70-90% Mukitake mushrooms: 70-90% Beech mushroom: 70-90% 30 to 60 days after the start of cultivation, we determined whether or not normal fruiting bodies were obtained. In Table 1, ○ indicates that normal fruiting bodies were obtained, and × indicates that the fruiting bodies were malformed or had poor growth.

[0021] [Table 1]

[0022] As can be seen from Table 1, when Lion's Mane mushrooms were cultivated using the culture medium of the example, normal fruiting bodies were obtained. Furthermore, when cultivation of Lion's Mane mushrooms was continued, the yields shown in Table 2 were obtained. Note that the peak days refer to the number of days from the start of cultivation to the day when the daily yield was highest.

[0023] [Table 2]

Claims

1. A growing medium for Lion's Mane mushrooms, containing coffee grounds.

2. A method for cultivating Lion's Mane mushrooms, including cultivating Lion's Mane mushrooms using a culture medium containing coffee grounds.

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