Skin care products containing a protein matrix

Abstract

One or more techniques and / or systems are disclosed for formulating skin care products to reduce aging or repair damaged skin. One skin care product includes a blend of growth factors, peptides, and amino acids. Skin care products formed from a synthetic protein matrix are also disclosed. The synthetic protein matrix can mimic the regenerative effects of human placental proteins, which is desirable when applied to the skin because as skin loses its regenerative properties, people show signs of aging and their healing ability decreases. Exemplary skin care products disclosed herein can be applied to the skin to help reduce this skin aging process or to repair damaged skin.

Description

[Background technology]

[0001] (CROSS REFERENCE TO RELATED APPLICATIONS) This application claims priority to U.S. Patent Application No. 63 / 026,781, entitled SKIN CARE PRODUCTS CONTAINING PLACENTAL PROTEIN, filed May 19, 2020, and incorporated herein by reference.

[0002] Human skin loses its regenerative properties and begins to show signs of aging and reduced healing capabilities as humans age. This change can occur due to a number of molecular reactions that occur at the cellular level. This results in a lack of skin elasticity, resulting in wrinkles and / or severe scarring after trauma. Often, people use skin care products on their skin to help mitigate this typical aging process and / or in an attempt to reverse the signs of aging skin. Summary of the Invention

[0003] This Summary is provided to introduce a selection of concepts in a simplified form that are further described below in the Detailed Description. This Summary is not intended to identify key elements or essential features of the claims, nor is it intended to be used to limit the scope of the claims.

[0004] One or more of the techniques and systems described herein can be utilized to produce a skin care product that includes an exemplary skin care blend. The skin care blend can have desirable characteristics that can reduce signs of aging or regenerate damaged skin. In one example, a skin care product can be produced that includes a synthetic protein matrix that mimics the regenerative properties of placental proteins. In this example, the skin care product can be applied to the skin to help reduce the skin's natural aging process or to heal damaged skin. The synthetic protein matrix can have certain desirable regenerative properties similar to those found in human placental proteins.

[0005] In one embodiment, the skin care blend comprises a synthetic protein matrix comprising sh-oligopeptide-2, sh-polypeptide-1, sh-polypeptide-9, sh-polypeptide-11, and sh-oligopeptide-1, a group of peptides comprising acetyl hexapeptide-8, palmitoyl tripeptide-5, and palmitoyl tetrapeptide-7, and a group of amino acids comprising acetyl glutamine, sodium hyaluronate, lysine HCL, alanine, histidine HCL, arginine, serine, proline, glutamic acid, threonine, valine, leucine, glycine, isoleucine, phenylalanine, taurine.

[0006] In another embodiment, the synthetic protein matrix is ​​produced from microbial biofermentation.

[0007] In another embodiment, a skin care product comprises the above skin care blend and glycerin, Alpinia arbutus root extract, tripeptide-1, polygonum aviculare extract, honey, nonapeptide-1, acetyl heptapeptide-4, phytosphingosine, sea whip extract, niacinamide, hydrolyzed hyaluronic acid, aminobutyric acid, adenosine, thioctic acid, glycolic acid, folic acid ferment extract, polysorbate 20, allantoin, caprylyl glycol, lecithin, butylene glycol, 1,2-hexanediol, dextran, phenoxyethanol, ethylhexylglycerin, and xanthan gum.

[0008] In another embodiment, the skin care product further comprises water and pentylene glycol.

[0009] In another embodiment, the skin care product is a face serum.

[0010] In another embodiment, the skin care product is configured to be applied to the user's face in the morning and evening to achieve desired results.

[0011] In another embodiment, the skin care product further comprises manuka honey.

[0012] In one embodiment, a skin care product comprises the above skin care blend in combination with water, ethylhexyl palmitate, butyrospermum parkii butter, coconut oil, cetearyl olivate, sorbitan oleate, stearyl alcohol, propanediol, glyceryl stearate, aloe barbadensis leaf juice, caprylhydroxamic acid, caprylyl glycol, glycerin, alteromonas ferment extract, glycosphingolipids, glycolipids, hydrolyzed hyaluronic acid, ferulic acid, retinyl palmitate, resveratrol, ergothioneine, linum usitatissimum seed oil ... oil), bacillus / soy / folic acid ferment extract, tocopherol, sodium PCA, 1,2-hexanediol, polysorbate 20, ethylhexylglycerin, allantoin, lecithin, butylene glycol, rosemary leaf extract, lavender oil, and xanthan gum.

[0013] In another embodiment, the skin care product is a moisturizer.

[0014] In another embodiment, the skin care product is configured to be applied to the user's face in the morning and evening to achieve desired results.

[0015] In one embodiment, a skin care product comprises the above skin care blend in combination with water, zinc oxide, canola extract, dimethicone, cyclopentasiloxane, caprylic triglyceride, glycerin, propanediol, bentonite, cetearyl alcohol, montmorillonite, illite, sodium chloride, xanthan gum, citric acid, manuka honey, polyglyceryl-8 oleate, polyhydroxystearic acid, dimethicone / vinyl dimethicone crosspolymer, lecithin, bacilus / folic acid / soy ferment extract, caprylyl glycol, butylene glycol, 1,2-hexanediol, caprylyl glycol, allantoin, hyaluronic acid, dimethylmethoxy chromanyl palmitate, caesalpinia spinosa fruit pod extract, helianthus annuus extract, and the like. sprout extract), maltodextrin, lactic acid, phenoxyethanol, ethylhexylglycerin.

[0016] In another embodiment, the skin care product is a sunscreen product.

[0017] In another embodiment, the skin care product is configured to be applied to a user's face in the morning to achieve desired results.

[0018] In one embodiment, a skin care product comprises a synthetic protein matrix and a natural protein matrix, the synthetic protein matrix comprising epidermal growth factor, insulin-like growth factor-1, acidic fibroblast growth factor, basic fibroblast growth factor, vascular endothelial growth factor, vitamin B9, and acetylglutamine.

[0019] In another embodiment, the synthetic protein matrix is ​​produced from microbial biofermentation.

[0020] In another embodiment, the natural protein matrix is ​​synthesized from human tissue conditioned media.

[0021] In another embodiment, the natural protein matrix comprises human fibronectin, human collagen, human thrombospondin, human cytoplasmic actin, and human elastin.

[0022] In another embodiment, the skin care product further comprises manuka honey.

[0023] In another embodiment, the skin care product further comprises shea butter, coconut oil, aloe, flaxseed oil, ergothioneine, vitamin A, vitamin C, vitamin E, resveratrol, coenzyme Q10, and ferulic acid.

[0024] In one embodiment, a skin care product comprises a skin care blend comprising water, pentylene glycol, glycerin, Alpinia arbutifolia Root Extract, Tripeptide-1, Salix nigra Extract, Honey, Nonapeptide-1, Acetyl Heptapeptide-4, Phytosphingosine, Sea Whip Extract, Niacinamide, Hydrolyzed Hyaluronic Acid, Aminobutyric Acid, Adenosine, Thioctic Acid, Glycolic Acid, Folic Acid Ferment Extract, Polysorbate 20, Allantoin, Caprylyl Glycol, Lecithin, Butylene Glycol, 1,2-Hexanediol, Dextran, Phenoxyethanol, Ethylhexylglycerin, and Xanthan Gum. and the skin care blend comprises a synthetic protein matrix comprising sh-oligopeptide-2, sh-polypeptide-1, sh-polypeptide-9, sh-polypeptide-11, and sh-oligopeptide-1; a group of peptides comprising acetyl hexapeptide-8, palmitoyl tripeptide-5, and palmitoyl tetrapeptide-7; and a group of amino acids comprising acetyl glutamine, sodium hyaluronate, lysine HCL, alanine, histidine HCL, arginine, serine, proline, glutamic acid, threonine, valine, leucine, glycine, isoleucine, phenylalanine, and taurine. To the accomplishment of the foregoing and related ends, the following description and the annexed drawings set forth certain illustrative aspects and embodiments. These are indicative of but a few of the various ways in which one or more aspects may be employed. Other aspects, advantages, and novel features of the present disclosure will become apparent from the following detailed description when considered in conjunction with the accompanying drawings. [Brief description of the drawings]

[0025] [Figure 1] FIG. 1 is a schematic diagram of an exemplary skin care blend utilizing one or more techniques and / or one or more systems described herein.

[0026] [Diagram 2]FIG. 2 is a schematic diagram of an exemplary skin care product utilizing one or more of the techniques and / or one or more of the systems described herein.

[0027] [Diagram 3] FIG. 3 is a schematic diagram of an exemplary skin care product utilizing one or more of the techniques and / or one or more of the systems described herein.

[0028] [Figure 4] FIG. 4 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0029] [Diagram 5] FIG. 5 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0030] [Figure 6] FIG. 6 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0031] [Figure 7] FIG. 7 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0032] [Figure 8] FIG. 8 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0033] [Figure 9] FIG. 9 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0034] [Figure 10] FIG. 10 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0035] [Figure 11] FIG. 11 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0036] [Figure 12] FIG. 12 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0037] [Figure 13] FIG. 13 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0038] [Figure 14] FIG. 14 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0039] [Figure 15] FIG. 15 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0040] [Figure 16] FIG. 16 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0041] [Figure 17]FIG. 17 is a chart illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0042] [Figure 18] FIG. 18 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0043] [Figure 19] FIG. 19 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0044] [Figure 20] FIG. 20 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0045] [Figure 21] FIG. 21 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0046] [Figure 22] FIG. 22 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0047] [Figure 23] FIG. 23 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0048] [Figure 24] FIG. 24 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0049] [Diagram 25] FIG. 25 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0050] [Figure 26] FIG. 26 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0051] [Figure 27] FIG. 27 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0052] [Figure 28] FIG. 28 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0053] [Figure 29] FIG. 29 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein.

[0054] [Diagram 30] FIG. 30 is a graph illustrating example results of one or more techniques and / or one or more system embodiments described herein. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0055] The claimed subject matter will now be described with reference to the drawings. Like numerals are generally used to refer to like elements throughout. In the following description, for purposes of explanation, numerous specific details are set forth in order to provide a thorough understanding of the claimed subject matter. It will be apparent, however, that the claimed subject matter may be practiced without these specific details. In other instances, structures and devices are shown in block diagram form to facilitate description of the claimed subject matter.

[0056] As humans age, human skin loses its regenerative properties and begins to show signs of aging, with a reduced ability to heal. This change can occur due to a number of molecular reactions that occur at the cellular level. As a result, skin elasticity can be lacking, and wrinkles and / or severe scarring after trauma can occur. Provided herein are exemplary skin care blends and / or skin care products that can reduce the skin aging process and / or restore damaged skin.

[0057] In one embodiment, the exemplary skin care blend can include a synthetic protein matrix, a plurality of peptides, a plurality of amino acids, and other ingredients. In this embodiment, the synthetic protein matrix can include a combination of synthetic growth factors and other ingredients. The exemplary skin care blend can be referred to as a formulation of peptide-rich plasma.

[0058] In this embodiment, specially formulated growth factors, essential vitamins, peptides, and amino acids are used to formulate the exemplary skin care blend. The combination of ingredients can help stressed or damaged cells grow healthier and healthier, resulting in healthier and younger looking skin. For example, the exemplary skin care blend can restore aged, stressed, and damaged cells to a healthy growth state more effectively than other methods. The rejuvenation rate of cells can be proportional to the concentration of the exemplary skin care blend, suggesting that the exemplary skin care blend plays a direct role in cell regeneration.

[0059] In another exemplary embodiment, the exemplary skin care blend may include a synthetic protein matrix, a natural protein matrix, and additional ingredients. This exemplary skin care blend may be referred to as an active placental matrix.

[0060] In this embodiment, synthetic placental proteins (synthetic protein matrix) can be used in the exemplary skin care product. Human placental proteins have gained popularity in recent years due to their potential regenerative effects. The effects of placental proteins may be desirable because as skin loses its regenerative properties, it begins to show signs of aging and its healing ability decreases. The synthetic protein matrix can exhibit the regenerative effects of human placental proteins while also mitigating (e.g., eliminating or reducing) the transmission of risk diseases that may be associated with human placental proteins. For example, the exemplary skin care blend can be generated from a combination of naturally-occurring compounds, amino acids, peptides, and the like. The exemplary skin care blend can include a protein matrix or growth factors, a group of peptides, and a group of amino acids. The skin care blend can improve skin condition, combat signs of aging, and / or improve skin regeneration after injury.

[0061] In some embodiments, the above exemplary skin care blend can be combined with other ingredients to form an exemplary skin care product.The exemplary skin care product can be in the form of serum, moisturizer, eye cream, sunscreen (SPF), or similar skin care product.As an example, the exemplary skin care product can be used by applying it directly to the skin and gently rubbing the product for absorption, promoting healthy skin and reducing the appearance of fine lines and wrinkles.

[0062] Referring to FIG. 1, a schematic diagram of an exemplary skin care blend 100 is shown. The skin care blend 100 can include a synthetic protein matrix 102, a plurality of peptides 104, a plurality of amino acids 106, and other ingredients 108. In this exemplary embodiment, the synthetic protein matrix 102 can include the following combination of growth factors: sh-oligopeptide-2, sh-polypeptide-1, sh-polypeptide-9, sh-polypeptide-11, and sh-oligopeptide-1. The plurality of peptides 104 can include palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, and acetyl hexapeptide-8. The plurality of amino acids 106 can include the following components: acetyl glutamine, lysine HCL, alanine, histidine HCL, arginine, serine, proline, glutamic acid, threonine, valine, leucine, glycine, isoleucine, phenylalanine, and taurine. The other ingredients 108 can include vitamin B-9 and sodium hyaluronate. The exemplary skin care blend 100 may be referred to as a peptide-rich plasma formulation.

[0063] In one embodiment, the exemplary skin care product 200 can be a face serum. The exemplary skin care product 200 (e.g., a face serum) can include the exemplary skin care blend 100 and additional skin care ingredients 202. The additional skin care ingredients 202 can include a selection of vitamins and / or other beneficial skin care ingredients. In this embodiment, the additional skin care ingredients 202 include glycerin, Alpinia arbutifolia root extract, tripeptide-1, willow extract, honey, nonapeptide-1, acetyl heptapeptide-4, phytosphingosine, sea whip extract, niacinamide, hydrolyzed hyaluronic acid, aminobutyric acid, adenosine, thioctic acid, glycolic acid, bacillus / soybean / folic acid ferment extract, polysorbate 20, allantoin, caprylyl glycol, lecithin, butylene glycol, 1,2-hexanediol, dextran, phenoxyethanol, ethylhexylglycerin, and xanthan gum.

[0064] In this embodiment, the exemplary skin care blend 100 (e.g., peptide-rich plasma) may contain active ingredients to promote healthy skin. However, it should be understood that other active ingredients may be added to improve the effectiveness of the exemplary skin care product 100. For example, other active ingredients may include Nonapeptide-1, Alpha Lipoic Acid, Niacinamide, Savage Ginger Root Extract and Knotgrass Flavonoids, Gamma Aminobutyric Acid (GABA), Manuka Honey, Glycolic Acid, and Hyaluronic Acid.

[0065] As an example, nonapeptide-1 can block melanin synthesis to target discoloration. Alpha lipoic acid can act as a powerful antioxidant and free radical scavenger. Niacinamide can reduce redness and inflammation. Wild ginger root extract and Niwa-yagi flavonoids can protect against environmental UV, IR, or blue light damage. Additionally, gamma aminobutyric acid (GABA) can include active neuropeptides for healing and repair. Manuka honey can promote healing and skin immunity. Glycolic acid can improve skin texture and reduce enlarged pores. Hyaluronic acid can improve skin plumpness and hydration. It should be understood that other ingredients selected according to sound professional judgment can be utilized with the exemplary skin care blend 100 to form a skin care product (e.g., skin care product 200) without departing from the scope of this application.

[0066] In one embodiment, the exemplary skin care product 200 may be a moisturizer. The exemplary skin care product 200 (e.g., a moisturizer) may include the exemplary skin care blend 100 and additional skin care ingredients 202. Additional ingredients 202 can include Water (Aqua / Water / Eau), Ethylhexyl Palmitate, Butyrospermum Parkii (Shea) Butter, Cocos Nucifera (Coconut) Oil, Cetearyl Olivate, Sorbitan Oleate, Stearyl Alcohol, Propanediol, Glyceryl Stearate, Aloe Barbadensis (Aloe) Leaf Juice, Caprylhydroxamic Acid, Caprylyl Glycol, Glycerin, Alteromonas Ferment Extract, Glycosphingolipids, Glycolipids, Hydrolyzed Hyaluronic Acid, Ferulic Acid, Retinyl Palmitate, Resveratrol, Ergothioneine, Linum Insect (Linseed) Seed Oil, Bacillus / Soybean / Folic Acid Ferment Extract, Tocopherol, Sodium PCA, 1,2-Hexanediol, Polysorbate 20, Ethylhexylglycerin, Allantoin, Lecithin, Butylene Glycol, Rosemary Leaf Extract, Lavender Oil, and Xanthan Gum. In this embodiment, the skin care product 200 (e.g., a moisturizer) can be applied to cleansed skin in the morning and evening, or as needed, and the user can massage it into the desired area of ​​the skin until fully absorbed.

[0067] In another embodiment, the exemplary skin care product 200 may be an eye renewal cream. The exemplary skin care product 200 (e.g., an eye renewal cream) may include the exemplary skin care blend 100 and additional skin care ingredients 202. The additional ingredients 202 may be water, pentylene glycol, methylheptyl isostearate, beta glucan, niacinamide, trehalose, dipeptide diaminobutyroyl benzylamide diacetate, sodium acrylate / sodium acryloyldimethyl taurate copolymer, boron nitride, glycerin, hesperidin, methyl chalcone, dipeptide-2, N-hydroxysuccinimide, chrysin, palmitoyl tripeptide-1, ascorbyl aminopropyl phosphate, dimethyl methoxychromanyl palmitate, alpha arbutin, kojic acid, arnica montana flower extract, bilberry (bilberry) fruit extract, acai fruit extract, wolfberry (goji) ... Extract), mangosteen peel, powder, pomegranate fruit powder, citrus citrifolia leaf powder, biotin, allantoin, bacillus / soy / folic acid ferment extract, tocopherol, steareth-20, chlorhexidine digluconate, potassium sorbate, citric acid, sodium citrate, phenoxyethanol, ethylhexylglycerin, tocopherol acetate, lecithin, propanediol, caprylyl glycol, and xanthan gum. In this embodiment, the skin care product 200 (e.g., eye renewal cream) can be applied by gently applying a pea-sized amount of the skin care product 200 under and around the eyes. Application can be performed every morning and in the evening.

[0068] In one embodiment, the exemplary skin care product 200 may be an SPF product. The exemplary skin care product 200 (e.g., an SPF product) may include the exemplary skin care blend 100 and additional skin care ingredients 202. Additional ingredients 202 may include water, zinc oxide, canola extract, dimethicone, cyclopentasiloxane, caprylic / capric triglyceride, glycerin, propanediol, bentonite, cetearyl alcohol, montmorillonite, illite, sodium chloride, xanthan gum, citric acid, manuka honey, polyglyceryl-8 oleate, polyhydroxystearic acid, dimethicone / vinyl dimethicone crosspolymer, lecithin, bacilus / folic acid / soybean ferment extract, caprylyl glycol, butylene glycol, 1,2-hexanediol, caprylyl glycol, allantoin, hyaluronic acid, dimethylmethoxychromanyl palmitate, caesalpinia spinosa pod extract, helianthus annuus sprout extract, maltodextrin, lactic acid, phenoxyethanol, and ethylhexylglycerin. In this embodiment, the skin care product 200 (e.g., an SPF product) can be applied to the face, neck, and chest in the morning. For example, two pumps of the product can be applied to the hands and gently massaged into the skin.

[0069] Referring to Figure 3, a schematic diagram of an exemplary skin care product 300 is shown. The skin care product 300 can be a skin moisturizer. The skin moisturizer includes a synthetic protein matrix 302, a natural protein matrix 304, and additional skin care ingredients 306. In this exemplary embodiment, the synthetic protein matrix 302 can include the following ingredients and growth factors: epidermal growth factor (EGF); insulin-like growth factor-1 (IGF-1), acidic fibroblast growth factor (FGF), basic FGF, vascular endothelial growth factor (VEGF), vitamin B9, and acetylglutamine.

[0070] In this embodiment, the natural protein matrix 304 may be synthesized from human tissue conditioned medium. The natural protein matrix 304 may include human fibronectin, human collagen, human thrombospondin, human actin cytoplasm, and human elastin. The natural protein matrix 304 may be combined with other components, such as the synthetic protein matrix 302, to form the exemplary skin care product 300. It is understood that the natural protein matrix 304 may be utilized in combination with the synthetic protein matrix 302, the skin care blend 100, the skin care product 200, any other skin care product, or may not be used at all.

[0071] For example, in the embodiments described herein, the exemplary skin care product 300 can include a synthetic protein matrix 302 produced from microbial biofermentation. The synthetic protein matrix 302 can include epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), acidic fibroblast growth factor (FGF), basic FGF, vascular endothelial growth factor (VEGF), vitamin B9, and acetylglutamine. Additionally, in this embodiment, the exemplary skin care product 300 can include a natural protein matrix 304 including human fibronectin, human collagen, human thrombospondin, human actin cytoplasm, and human elastin. Additionally, the exemplary skin care product 300 can include any or all of the following additional ingredients 306: shea butter, coconut oil, aloe, linseed oil, ergothioneine, vitamin A, vitamin C, vitamin E, resveratrol, coenzyme Q10, and ferulic acid.

[0072] In other embodiments, the skin care products (e.g., 200, 300) can include additional nutritional ingredients or vitamins. For example, the additional ingredients 202, 306 can include any or all of shea butter, coconut oil, aloe, flaxseed oil, ergothioneine, vitamin A, vitamin C, vitamin E, resveratrol, coenzyme Q10, and ferulic acid. Further, the additional skin care ingredients 202, 306 can include glycerin, Alpinia arbutifolia root extract, tripeptide-1, willow extract, honey, nonapeptide-1, acetyl heptapeptide-4, phytosphingosine, sea whip extract, niacinamide, hydrolyzed hyaluronic acid, aminobutyric acid, adenosine, thioctic acid, glycolic acid, bacillus / soy / folic acid ferment extract, polysorbate 20, allantoin, caprylyl glycol, lecithin, butylene glycol, 1,2-hexanediol, dextran, phenoxyethanol, ethylhexylglycerin, and / or xanthan gum. It should be understood that other nutritional ingredients or vitamins can be added to the skin care products 200, 300 according to sound professional judgment without departing from the scope of this application.

[0073] In one example, human placental protein can have desirable regenerative properties when used on human skin.However, the use of human placental tissue is restricted in certain countries due to the risk of disease transmission.Other countries allow the use of human placental protein in skin care products, but this process can be complicated.This complication can make the use of human placental protein undesirable.For example, human placental protein contains carcinogenic estrogens.In view of these issues, a process that captures the regenerative properties of human placental protein without the risks associated with its use may be desirable in skin care and / or medical fields.

[0074] In an exemplary embodiment, a portion of the placental tissue containing skin actives (e.g., a protein-rich mixture of peptides, growth factors, etc.) can be isolated from a portion of the inactive placental tissue (e.g., cells, collagen, etc.). In this manner, a portion of the active beneficial cells can be isolated from the inactive tissue / cells. As an example, the active portion of the isolated tissue contains desirable growth factors and proteins, but does not contain cells, collagen, estrogen, or other undesirable moieties. In this embodiment, the synthetic protein matrix can be derived from an isolated portion of the active placental proteins.

[0075] In one embodiment, the synthetic protein matrix 302 is formed through a manufacturing process that results in a series of growth factors. For example, the growth factors and / or synthetic protein matrix can be manufactured using human tissue cultured media and through microbial biofermentation. In this embodiment, genes are synthesized by polymerase chain reaction, where the genes are injected with recombinant E. coli, which causes growth and fermentation. This material can then be purified to obtain the desired growth factors. Using this process, a synthetic matrix of proteins, peptides, immunostimulants, and growth factors can be formulated that mimic the same protein composition as human placental tissue. By mimicking the active protein composition of live human placental tissue, the synthetic protein matrix 302 can achieve similar regenerative and anti-aging benefits, if not enhanced.

[0076] In the embodiments described herein, the growth factor can achieve the desired biological effect on human skin. For example, some growth factors have been confirmed to be useful for wound healing and / or epidermal remodeling. For example, transforming growth factor-β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) can be effective on human skin. At least one growth factor can be utilized in the skin care product since the growth factor can achieve the desired regenerative effect on human skin. It should be understood that at least one or more growth factors can be used. For example, the following exemplary growth factors can be used: EGF, PDGF, FGF, TGF-β, TGF-α, NGF, Epo, IGF-I, IGF-II, IL1-α, IL-1-β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, INF-α, INF-β, INF-γ, TNF-α, TNF-β, GM-CSF, and M-CSF, or combinations thereof. In an exemplary embodiment, the growth factor combination can be formed from epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), acidic fibroblast growth factor (FGF), basic FGF, vascular endothelial growth factor (VEGF).

[0077] In one embodiment, the essential vitamin used in the skin care blend is B9-Vitapol. In this embodiment, the formulation of B9-Vitapol is made through a structural change of folic acid to improve the pH sensitivity and low solubility of folic acid.

[0078] In another embodiment, the essential amino acid used in the skin care blend is Hydracell Q. In this embodiment, acetylated glutamine is reformulated to improve stability, resulting in Hydracell Q.

[0079] In one embodiment, the exemplary skin care blend 100 can be made into a serum (e.g., skin care product 200) using the technology described herein. In this embodiment, other nourishing and healing products can be combined with the skin care blend 100 to further promote skin repair and the reversal of visible signs of aging. As an example, the exemplary skin care blend 100 or skin care product 200 can be combined with manuka honey to provide additional skin nourishment.

[0080] In one embodiment, the exemplary skin care blend 100 can be utilized to incorporate moisturizers, where products that promote water absorption are combined with the skin care blend 100 or synthetic protein matrix 302 to achieve moisturized skin and promote skin elasticity. As an example, the exemplary skin care product blend 100 or synthetic protein matrix 303 can be combined with hyaluronic acid and shea butter to increase cellular hydration and stimulation.

[0081] In one embodiment, the exemplary skin care product 300 is a moisturizer. In this embodiment, the moisturizer is applied topically to the skin in the morning and / or evening. Similarly, the exemplary skin care product 200 can be a face serum. In this embodiment, the serum is applied topically to the skin in the morning and / or evening. Further, with respect to the serum, the user can take one pipette length of serum on the fingertips and gently apply it to the face and neck in an upward motion. Or, in some embodiments, the user can take 4-5 drops of serum on the fingertips and gently massage it into the face. For best effect, when using serum and moisturizer at the same time, the user can allow the serum to absorb into the skin before applying the moisturizer.

[0082] As an example, the exemplary skin care blends (e.g., skin care blend 100) and / or skin care products (e.g., skin care products 200, 300) described herein can have both medical and cosmetic applications other than those described above. For example, with respect to medical applications, the products described herein may be applied to wound healing, non-healing ulcers, scars after skin cancer, scars after radiation, etc. With respect to cosmetic applications, the products described herein may be beneficial when applied after microneedle treatment or laser resurfacing. It is understood that the examples provided herein are meant to be non-limiting and other similar applications may exist.

[0083] A clinical efficacy and consumer perception evaluation was conducted to evaluate the effect of the exemplary skin care product 200 on the skin of a user. The results of the clinical trial are shown in Figures 5 to 24. In this clinical trial, the exemplary skin care product 200 is a face serum. The results show that the face serum can achieve a nourishing and regenerating effect on the skin of a user. The face serum analyzed contained water, pentylene glycol, exemplary skin care blend 100 (e.g., peptide rich plasma), glycerin, Alpinia arbutus root extract, tripeptide-1, Mitsia oleracea extract, honey, nonapeptide-1, acetyl heptapeptide-4, phytosphingosine, sea whip extract, niacinamide, hydrolyzed hyaluronic acid, aminobutyric acid, adenosine, thioctic acid, glycolic acid, bacillus / soy / folic acid ferment extract, polysorbate 20, allantoin, caprylyl glycol, lecithin, butylene glycol, 1,2-hexanediol, dextran, phenoxyethanol, ethylhexylglycerin, and xanthan gum. The synthetic protein matrix includes sh-oligopeptide-2, sh-polypeptide-1, sh-polypeptide-9, sh-polypeptide-11, acetyl hexapeptide-8, sh-oligopeptide-1, palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, acetylglutamine, sodium hyaluronate, lysine HCL, alanine, histidine HCL, arginine, serine, proline, glutamic acid, threonine, valine, leucine, glycine, isoleucine, phenylalanine, and taurine.

[0084] In the clinical trial, 30 female participants aged 35-65 years underwent a four-week clinical trial. The study was conducted in accordance with the spirit and purpose of the Good Clinical Practice regulations set forth in Title 21 of the United States Code of Federal Regulations. Participants were required to complete a questionnaire regarding skin care products at the end of the four-week period.

[0085] In this study, subjects (participants) were enrolled according to the following inclusion or exclusion criteria: women aged 35-65 years who were generally in good health (no physical requirements), who were able to read, understand, and sign the informed consent form, who could return to the clinic at the required intervals, who had the expected ability to follow the study instructions, participate in the study, return to all appointments, and apply the product according to the instructions, and who were regular users of face products. Exclusion criteria included women who were pregnant, planning to become pregnant, breastfeeding and / or raising children, those with visible skin diseases, those who were sunburned, had a sunburn on their face, or were planning a vacation involving sunbathing or planning to use a tanning booth during the study period, those engaged in a concurrent research project of facial products, those taking medications that may interfere with the study results including use of steroidal / nonsteroidal anti-inflammatory drugs or antihistamines, those who had undergone a chemical face peel (deep peel in the past year, superficial peel in the past 2 months), or laser resurfacing or skin peeling procedure on the face in the past 2 years, those with acne, active atopic dermatitis / eczema or psoriasis, those who had undergone a surgical cosmetic procedure on the face within the past 10 years, those receiving treatment or history of cancer of any kind, those receiving treatment for asthma or diabetes, and those with sensitivities to cosmetics or personal care products.

[0086] The study was designed as a 4-week study in which participants applied the test serum according to the instructions provided by the study sponsor. Participants reported to the study facility at the start of the study. At the study facility, a trained technician performed a skin test on the participants' skin firmness and elasticity. Participants then applied the test serum daily (morning and evening) for 4 weeks. At the end of the 4-week period, participants reported back to the study facility for final measurements and evaluations. Participants also completed a survey / questionnaire to evaluate the test serum. The questionnaire presented specific questions about the use of the product and asked participants to respond according to the answer options (strongly agree, agree, slightly agree, disagree, strongly disagree).

[0087] Participants were given a diary and specific application instructions. Each diary entry was to include the date and time the product was used and any comments or observations. The instructions stated that participants should place 4-5 drops on their fingertips and gently apply to the face. The instructions also stated that participants should not use the study skin care product with any other serums or any new skin care products (e.g., products not used by the participant prior to the study).

[0088] The results of the clinical trials described above, as shown in Figures 5-24, indicated that the exemplary embodiment of the face serum had multiple benefits on the user / patient's skin. For example, 100% of the clinical participants agreed that the serum helped smooth fine lines and wrinkles. 95% of the clinical participants agreed that the serum helped reduce pore size. 99% of the clinical participants stated that their skin looked less dull. 96% of the clinical participants agreed that the serum helped their skin look brighter. 96% of the clinical participants agreed that the serum gave their skin an instant glow. 95% of the clinical participants stated that their skin looked more radiant. Other results included 89% of the clinical participants agreed that the serum helped to even out skin tone. 90% of the clinical participants stated that their skin looked plumper. 96% of the clinical participants stated that their skin looked more hydrated. 96% of clinical participants stated that their skin appeared plumper and more hydrated. In further results, 100% of clinical participants agreed that their skin appeared firmer and firmer. 82% of clinical participants stated that their skin appeared to have less sun damage. 84% of clinical participants stated that their skin showed less signs of sun damage and fewer environmental stressors. 87% of clinical participants agreed that the serum helped reduce the appearance of sun damage and environmental stressors.

[0089] As an example, a second clinical efficacy and consumer perception evaluation was conducted to evaluate the effect of the exemplary skin care product 200 on the skin of a user. The results of the second clinical trial are shown in Figures 25-30. In this clinical trial, the exemplary skin care product 200 is an eye renewal cream. The results show that the eye renewal cream can achieve a nourishing and regenerative effect on the skin of a user. The analyzed eye cream contains the exemplary skin care blend 100 (peptide-rich plasma), water, pentylene glycol, methylheptyl isostearate, beta-glucan, niacinamide, trehalose, dipeptide diaminobutyroyl benzylamide diacetate, sodium acrylate / sodium acryloyldimethyl taurate copolymer, boron nitride, glycerin, hesperidin, methyl chalcone, dipeptide-2, N-hydroxysuccinimide, chrysin, palmitoyl tripeptide-1, ascorbyl aminopropyl phosphate, dimethyl methoxychromanyl palmitate, Contains alpha arbutin, kojic acid, arnica montana flower extract, vaccinium myrtillus (bilberry) fruit extract, acai fruit extract, wolfberry (goji) fruit extract, mangosteen peel and powder, pomegranate fruit powder, mori citronellol leaf powder, biotin, allantoin, bacillus / soy / folic acid ferment extract, tocopherol, steareth-20, chlorhexidine digluconate, potassium sorbate, citric acid, sodium citrate, phenoxyethanol, ethylhexylglycerin, tocopheryl acetate, lecithin, propanediol, caprylyl glycol, xanthan gum.

[0090] In this test, for example, 97% of the participants agreed that the eye renewal cream helped to reduce dark circles under the eyes. 81% of the participants agreed that the eye renewal cream helped to reduce puffiness around the eyes. 91% of the participants agreed that the eye renewal cream helped to smooth fine lines and wrinkles. Furthermore, in this test, 81% of the participants agreed that the skin around the eyes was visibly firmer. 84% of the participants agreed that the eye renewal cream helped to give a plump and moisturized appearance. 78% of the participants agreed that the eye renewal cream made the skin around the eyes look brighter. As described above, the clinical results show that the exemplary skin care product 200 (e.g., eye cream) can have a number of positive and desirable effects on the skin around the user's eyes.

[0091] In one embodiment, the exemplary skin care product 300 is shown to be more effective than current, commercially available, or human placenta products. As shown in FIG. 4, side-by-side testing results provide improved results in cell proliferation. Graph 400 shows that as the concentration 406 of placental protein increases in the exemplary placenta product (e.g., skin care product 300), an increase in cell growth rate 408 is shown. The growth rate of the synthetic protein matrix 402 is shown to be greater than the current commercially available placenta product 404. In this embodiment, the cell line is Human Derma Fibroblast neonatal and the detection method is MTT assay. In this embodiment, the exemplary skin care product 402 stimulates cell proliferation more than the commercially available placenta product 404.

[0092] Furthermore, the term "exemplary" is used herein to mean serving as an example, instance, or illustration. Any aspect or design described herein as "exemplary" should not necessarily be construed as advantageous over other aspects or designs. Rather, use of the word "exemplary" is intended to present concepts in a concrete manner. As used in this application, the term "or" is intended to mean an inclusive "or" rather than an exclusive "or". That is, unless otherwise specified or clear from the context, "X uses A or B" is intended to mean any of the natural inclusive permutations. That is, if X uses A, if X uses B, if X uses both A and B, then "X uses A or B" is satisfied based on any of the aforementioned cases. Furthermore, at least one of A and B, etc. generally means A or B, or both A and B. Additionally, the articles "a" and "an" as used in this application and the appended claims may generally be construed to mean "one or more" unless otherwise specified or clear from the context to be intended in the singular form.

[0093] Although the subject matter has been described in language specific to structural features and / or methodological acts, it is to be understood that the subject matter defined in the appended claims is not necessarily limited to the specific features or acts described above. Rather, the specific features and acts described above are disclosed as example forms of implementing the claims.

[0094] Also, while the present disclosure has been shown and described with respect to one or more embodiments, equivalent alternatives and modifications will be apparent to those skilled in the art based on reading and understanding the present specification and the accompanying drawings. The present disclosure includes all such modifications and alternatives, and is limited only by the scope of the following claims. In particular, with respect to the various functions performed by the components (e.g., elements, resources, etc.) described above, the terms used to describe such components are intended to correspond to any components (e.g., functionally equivalent components) that perform the specified functions of the described components, but are not structurally equivalent to the disclosed structures that perform the functions in the exemplary embodiments of the present disclosure described herein, unless otherwise indicated. In addition, although a particular feature of the present disclosure may be disclosed with respect to only one of several embodiments, such feature may be combined with one or more other features of other embodiments, as may be desirable and advantageous for any given or particular application. Furthermore, to the extent that the terms "includes," "having," "has," "with," or variations thereof are used in either the detailed description or the claims, such terms are intended to be inclusive in the same manner as the term "comprising."

[0095] Moreover, the claimed subject matter may be implemented as a method, apparatus, or article of manufacture using standard engineering techniques produced to practice the disclosed subject matter. Of course, those skilled in the art will recognize that many modifications can be made to this configuration without departing from the scope or spirit of the claimed subject matter. The above embodiments have been described. It will be apparent to those skilled in the art that the above methods and apparatuses may incorporate changes and modifications without departing from the general scope of the invention. It is intended to include all such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. 1. A skin care product comprising a synthetic protein matrix and optionally a natural protein matrix, The synthetic protein matrix comprises: Epidermal growth factor, Insulin-like growth factor-1, Acidic fibroblast growth factor, Basic fibroblast growth factor; Vascular endothelial growth factor; Vitamin B9 and acetylglutamine, The skin care product further comprises glycerin, Alpinia arbutus root extract, tripeptide-1, Willow extract, honey, nonapeptide-1, acetyl heptapeptide-4, phytosphingosine, sea whip extract, niacinamide, hydrolyzed hyaluronic acid, aminobutyric acid, adenosine, thioctic acid, glycolic acid, folic acid ferment extract, polysorbate 20, allantoin, caprylyl glycol, lecithin, butylene glycol, 1,2-hexanediol, dextran, phenoxyethanol, ethylhexylglycerin, and xanthan gum; 10. A skin care product, wherein the natural protein matrix optionally included in the skin care product comprises human fibronectin, human collagen, human thrombospondin, human cytoplasmic actin, and human elastin.

2. A method for producing the skin care product of claim 1, comprising: A method of producing said synthetic protein matrix, comprising the step of producing said synthetic protein matrix from a microbial biofermentation.

3. A method for producing the skin care product of claim 1, comprising the steps of: The method comprises the step of synthesizing the native protein matrix from human tissue conditioned medium.

4. 10. The skin care product of claim 1 further comprising manuka honey.

5. 10. The skin care product of claim 1 further comprising shea butter, coconut oil, aloe, flaxseed oil, ergothioneine, vitamin A, vitamin C, vitamin E, resveratrol, coenzyme Q10, and ferulic acid.

6. 10. The skin care product of claim 1, comprising water, ethylhexyl palmitate, butyrospermum parkii butter, coconut oil, cetearyl olivate, sorbitan oleate, stearyl alcohol, propanediol, glyceryl stearate, aloe barbadensis leaf juice, caprylhydroxamic acid, alteromonas ferment extract, glycosphingolipids, glycolipids, ferulic acid, retinyl palmitate, resveratrol, ergothioneine, linum usitatissimum seed oil, bacillus / soybean ferment extract, tocopherol, sodium PCA, rosemary leaf extract, and lavender.

7. The skin care product of claim 6 , wherein the skin care product is a moisturizer.

8. 8. The skin care product of claim 7, wherein the skin care product is configured to be applied to a user's face in the morning and evening to achieve desired results.

9. 10. The skin care product of claim 1 comprising water, zinc oxide, canola extract, dimethicone, cyclopentasiloxane, caprylic triglyceride, propanediol, bentonite, cetearyl alcohol, montmorillonite, illite, sodium chloride, citric acid, manuka honey, polyglyceryl-8 oleate, polyhydroxystearic acid, dimethicone / vinyl dimethicone crosspolymer, bacillus / soybean ferment extract, hyaluronic acid, dimethylmethoxychromanyl palmitate, caesalpinia spinosa pod extract, sunflower sprout extract, maltodextrin, and lactic acid.

10. 10. The skin care product of claim 9, wherein the skin care product is a sunscreen product.

11. The skin care product of claim 10 , wherein the skin care product is configured to be applied to a user's face in the morning to achieve desired results.

12. The skin care product of claim 1, further comprising water and pentylene glycol.

13. The skin care product of claim 12, wherein the skin care product is a face serum.

14. The skin care product of claim 13, wherein the skin care product is configured to be applied to the user's face in the morning and evening to achieve desired results.

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