Method and apparatus for monitoring the concentration of a reagent, electrical device, and computer readable storage medium - Patents.com

Abstract

A method, apparatus, electrical device, and computer-readable storage medium for monitoring reagent concentrations are provided. [Solution] The method of the present invention includes obtaining a first concentration of the current reagent measured during filling of the current reagent having a set concentration, obtaining a current volume of carryover determined in the process of filling and drawing out the previous reagent, obtaining a second concentration of the current reagent measured during drawing out the current reagent, and determining a third concentration of the current reagent based on the set concentration, the capacity of the reagent bottle and the current volume of carryover, updating the current volume of carryover based on the first concentration, the second concentration and the capacity of the reagent bottle, and if the third concentration is less than the set limit concentration, notifying replacement of the current reagent and / or refreshing the current reagent with a reagent of a higher concentration.

Description

[Technical field]

[0001] The present disclosure relates to the field of tissue processing technology, and more particularly to methods for monitoring the concentration of a reagent, apparatus, electrical devices and computer readable storage media for monitoring the concentration of a reagent. [Background technology]

[0002] The reagents need to meet a certain concentration level when used for tissue processing, and monitoring the concentration of the reagent is an important task. However, during tissue processing, the reagents are filled into or sucked out of the container, causing carryover remaining in the container, so the concentration changes and it is difficult to obtain the exact reagent concentration. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] China Patent Application Publication No. 111141564 Summary of the Invention

[0004] Embodiments of the present disclosure provide a method for monitoring reagent concentration, an apparatus for monitoring reagent concentration, an electrical device for monitoring reagent concentration, and a computer readable storage medium that can monitor reagent purity in real time and remind a user to replace the reagent in time, thereby improving the quality of tissue processing. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0005] In a first aspect of the embodiments of the present disclosure, a computer-implemented method for monitoring the concentration of a reagent is provided, the method including: obtaining a first concentration of a current reagent measured during filling of a current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path; obtaining a current volume of carryover remaining in the container and the liquid flow path determined during a process of filling a previous reagent into the container and drawing the previous reagent from the container; obtaining a second concentration of the current reagent measured during drawing of the current reagent from the container into the reagent bottle through the liquid flow path and determining a third concentration of the current reagent after drawing the current reagent from the container into the reagent bottle based on the preset concentration, the volume of the reagent bottle and the current volume of carryover; updating the current volume of carryover remaining in the container and the liquid flow path based on the first concentration, the second concentration and the volume of the reagent bottle; and notifying a user to replace the current reagent and / or refresh the current reagent with a reagent of a higher concentration if the third concentration is less than a preset limit concentration.

[0006] In one embodiment, determining a third concentration of the current reagent after the current reagent is aspirated from the container to the reagent bottle based on the preset concentration, the volume of the reagent bottle, and the current volume of carry-over includes the steps of: determining a concentration of the current reagent in the container based on the preset concentration, the volume of the reagent bottle, and the current volume of carry-over after the current reagent is filled into the container; controlling the reagent bottle to replenish the current reagent having the preset concentration after the current reagent is aspirated from the container to the reagent bottle; and determining a third concentration of the current reagent in the reagent bottle based on the concentration of the current reagent in the container, the volume of the reagent bottle, the preset concentration, and the current volume of carry-over.

[0007] In one embodiment, determining the concentration of the current reagent in the container based on the preset concentration, the capacity of the reagent bottle, and the current volume of carryover includes: calculating the volume of the major component of the current reagent in the container using the formula: Vaf = (Vb - Vco) x Cbf; and calculating the concentration of the current reagent in the container using the formula: Caf = Vaf÷Vb; where Vaf represents the volume of the major component of the current reagent in the container, Vco represents the current volume of carryover, and Vb represents the current volume of carryover. Container capacity , and Cbf represents the preset concentration and Caf represents the current concentration of the reagent in the container.

[0008] In one embodiment, determining the third concentration of the current reagent in the reagent bottle based on the concentration of the current reagent in the container, the volume of the reagent bottle, the preset concentration, and the current volume of carry-over includes: calculating a volume of a major component of the current reagent in the reagent bottle using the formula: Vad = (Vb - Vco) x Caf + Vco x Cbf; and calculating the third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb; where Vad represents the volume of the major component of the current reagent in the reagent bottle, Vco represents the current volume of carry-over, and Vb represents the current volume of carry-over. Container capacity , Cbf represents the preset concentration, Caf represents the current concentration of the reagent in the container, and Cad represents the third concentration.

[0009] In one embodiment, updating the current volume of carryover remaining in the container and the fluid flow path based on the first concentration, the second concentration and the volume of the reagent bottle includes calculating an updated volume of carryover using the formula: Vco' = (CDF - CDD) / CDF x Vb; and replacing the current volume of carryover with the updated volume of carryover; where Vco' represents the updated volume of carryover, CDD represents the second concentration of the current reagent, CDF represents the first concentration of the current reagent, and Vb is Container capacity Represents.

[0010] In one embodiment, the current reagent is selected from the group including ethanol and xylene.

[0011] In a second aspect of embodiment(s) of the present disclosure, an apparatus for monitoring a concentration of a reagent is provided. The apparatus includes a first acquisition module configured to acquire a first concentration of the current reagent measured during filling of a current reagent having a preset concentration from the reagent bottle into the container through the liquid flow path; a second acquisition module configured to acquire a current volume of carryover remaining in the container and the liquid flow path determined in a process of filling a previous reagent into the container and aspirating the previous reagent from the container; a determination module configured to acquire a second concentration of the current reagent measured during aspirating of the current reagent from the container into the reagent bottle through the liquid flow path and to determine a third concentration of the current reagent after aspirating the current reagent from the container into the reagent bottle based on the preset concentration, the capacity of the reagent bottle and the current volume of carryover; an update module configured to update the current volume of carryover remaining in the container and the liquid flow path based on the first concentration, the second concentration and the capacity of the reagent bottle; and a notification module configured to notify a user to replace the current reagent if the third concentration is less than a preset limit concentration and / or a refresh module configured to refresh the current reagent with a reagent of a higher concentration.

[0012] In one embodiment, the determination module includes a first determination unit configured to determine a concentration of a current reagent in the container based on a preset concentration, a capacity of the reagent bottle, and a current volume of carry-over after the current reagent is filled into the container; a control unit configured to control the reagent bottle so that a current reagent having the preset concentration is replenished after the current reagent is drawn out from the container to the reagent bottle; and a second determination unit configured to determine a third concentration of the current reagent in the reagent bottle based on the concentration of the current reagent in the container, the capacity of the reagent bottle, the preset concentration, and the current volume of carry-over.

[0013] In one embodiment, the first determination unit includes a first calculation sub-module configured to calculate a volume of the major component of the reagent currently in the container using the formula: Vaf = (Vb - Vco) x Cbf; and a second calculation sub-module configured to calculate a concentration of the reagent currently in the container using the formula: Caf = Vaf÷Vb, where Vaf represents the volume of the major component of the reagent currently in the container, Vco represents the current volume of carryover, and Vb represents the concentration of the reagent currently in the container using the formula: Caf = Vaf÷Vb. Container capacity where Cbf represents the preset concentration and Caf represents the current concentration of the reagent in the container.

[0014] In one embodiment, the second determination unit includes a third calculation sub-module configured to calculate a volume of the major component of the current reagent in the reagent bottle using the formula: Vad = (Vb - Vco) x Caf + Vco x Cbf, and a fourth calculation sub-module configured to calculate a third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb, where Vad represents the volume of the major component of the current reagent in the reagent bottle, Vco represents the current volume of the carryover, and Vb represents the third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb, Container capacity where Cbf represents the preset concentration, Caf represents the current concentration of the reagent in the container, and Cad represents a third concentration.

[0015] In one embodiment, the update module includes a third determination unit configured to calculate an updated carry-over volume using the formula: Vco' = (CDF - CDD) / CDF x Vb; and a replacement unit configured to replace the current volume of the carry-over with the updated carry-over volume, where Vco' represents the updated carry-over volume, CDD represents the second concentration of the current reagent, CDF represents the first concentration of the current reagent, and Vb is the first concentration of the current reagent. Container capacity Represents.

[0016] In one embodiment, the current reagent is selected from the group including ethanol and xylene.

[0017] In a third aspect of an embodiment of the present disclosure, an electrical device is provided, the electrical device including a processor and a memory configured to store instructions executable by the processor, the instructions, when executed by the processor, causing the processor to perform the method of any of the embodiments of the present disclosure.

[0018] In a fourth aspect of an embodiment of the present disclosure, a non-transitory computer-readable medium is provided, the computer-readable medium including instructions stored therein that are executable by a processor, the instructions, when executed by the processor, causing the processor to perform the method of any of the embodiments of the present disclosure.

[0019] The method and apparatus for monitoring the concentration of a reagent can be used to obtain a first concentration of the current reagent measured during filling of a current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path; obtain a current volume of carryover remaining in the container and the liquid flow path determined in a process of filling a previous reagent into the container and drawing the previous reagent from the container; then obtain a second concentration of the current reagent measured during drawing of the current reagent from the container into the reagent bottle through the liquid flow path; determine a third concentration of the current reagent after the reagent is drawn from the container into the reagent bottle based on the preset concentration, the volume of the reagent bottle and the current volume of carryover; and update the current volume of carryover remaining in the container and the liquid flow path based on the first concentration, the second concentration and the volume of the reagent bottle; and notify a user to replace the current reagent if the third concentration is less than a preset limit concentration. In this manner, the volume of carryover remaining in the container can be dynamically updated so that the concentration of the reagent can be accurately determined in real time to notify the user to replace the reagent in time, thereby improving the quality of tissue processing. [Brief description of the drawings]

[0020] In order to clearly describe the technical solutions of the embodiments of the present disclosure, a brief description of the drawings used in the embodiments is given below. Obviously, the drawings in the following description are only a part of the embodiments of the present disclosure, and those skilled in the art can obtain other drawings according to these drawings without creative efforts. [Figure 1] FIG. 1 is a flow chart of a method for monitoring the concentration of a reagent according to some embodiments of the present disclosure. [Diagram 2] FIG. 2 is a flow chart illustrating a process for obtaining the concentrations of reagents in some embodiments of the present disclosure. [Diagram 3] FIG. 3 is a block diagram of an apparatus for monitoring the concentration of a reagent according to some embodiments of the present disclosure. [Figure 4] FIG. 4 is a block diagram of an electrical device according to some embodiments of the present disclosure.

[0021] [Embodiment] DETAILED DESCRIPTION OF EMBODIMENTS Below, embodiments are described in detail, examples of which are illustrated in the accompanying drawings. In the following description, reference is made to the accompanying drawings, in which the same numerals in different drawings represent the same or similar elements unless otherwise indicated. The following description of embodiments does not represent all examples consistent with the present disclosure. Rather, they are merely illustrative of apparatus and methods consistent with the aspects related to the present disclosure recited in the appended claims.

[0022] An embodiment of the present disclosure provides a method for monitoring the concentration of a reagent used for tissue processing, which may be ethanol and xylene. As shown in FIG. 1, the method of the present invention includes steps 101 to 105.

[0023] In step S101, a first concentration of a current reagent is obtained, which is measured during filling of a current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path.

[0024] In this embodiment, the concentration of the reagent can be measured by the concentration meter while the reagent is being filled from the reagent bottle through the liquid flow path into the container.

[0025] In step S102, the current volume of carryover remaining in the container and the fluid flow path, as determined during the process of filling and withdrawing the previous reagent into and from the container, is obtained.

[0026] Typically, there is carryover remaining in the container and the liquid flow path during the filling of the reagent into the container and the aspirating of the reagent from the container via the liquid flow path. In the case where the container has carryover remaining therein, the concentration of the reagent may change when the reagent is filled into the container and aspirated from the container via the liquid flow path. In order to monitor the concentration of the reagent, the current volume of the carryover remaining in the container and the liquid flow path is obtained. Since the current volume of the carryover remaining in the container and the liquid flow path is caused by the previous filling of the reagent into the container and the aspirating of the reagent from the container via the liquid flow path, the current volume of the carryover can be determined in the process of the previous filling of the reagent into the container and the previous aspirating of the reagent from the container.

[0027] In step S103, a second concentration of the current reagent measured during the aspirating of the current reagent from the container to the reagent bottle via the liquid flow path is obtained, and a third concentration of the current reagent after the current reagent is aspirated from the container to the reagent bottle is determined based on the preset concentration, the capacity of the reagent bottle, and the current volume of carryover.

[0028] After a reagent is filled into the container, the concentration of the reagent in the container changes relative to the concentration of the reagent during filling due to the current volume of carryover remaining in the container and the liquid flow path. Furthermore, after the reagent is drawn from the container through the liquid flow path into the reagent bottle, the current concentration of the reagent in the reagent bottle is not the same as the initial concentration of the reagent in the reagent bottle due to the current volume of carryover remaining in the container and the liquid flow path.

[0029] In some embodiments, a third concentration of the current reagent after the current reagent is aspirated from the container to the reagent bottle can be determined as follows: After the current reagent is filled into the container, a concentration of the current reagent in the container is determined based on the preset concentration, the volume of the reagent bottle, and a current volume of carryover remaining in the container. After the current reagent is aspirated from the container to the reagent bottle, the reagent bottle is refilled with the current reagent having the preset concentration. A third concentration of the current reagent in the reagent bottle is determined based on the concentration of the current reagent in the container, the volume of the reagent bottle, the preset concentration, and a current volume of carryover remaining in the container.

[0030] In some embodiments, after the reagent is filled into the container, the volume of a major component of the reagent in the container is determined by: Formula:Vaf = (Vb - Vco)×Cbf where Vaf represents the volume of the main component of the reagent currently in the container, Vco represents the current volume of the carryover remaining in the container, and Vb represents the volume of the major component of the reagent currently in the container. Container capacity and Cbf represents a preset concentration.

[0031] The volume of the main components and Container capacity Based on Formula: Caf = Vaf÷Vb The concentration of the reagent in the vessel can be calculated using: where Caf represents the current concentration of the reagent in the vessel.

[0032] In the container, a reagent can be used for tissue processing. After tissue processing, the reagent can be aspirated from the container back to the reagent bottle, and the reagent bottle is refilled with the current reagent having a preset concentration to ensure that the reagent bottle is filled with the current reagent, i.e., the volume of the current reagent in the reagent bottle is present. The concentration of the reagent in the reagent bottle is then determined as follows:

[0033] The volume of the major component of the reagent in the reagent bottle is Formula: Vad = (Vb - Vco) ×Caf + Vco×Cbf where Vad represents the volume of the major component of the reagent currently in the reagent bottle.

[0034] The concentration of the reagent in the reagent bottle is Formula: Cad = Vad÷Vb where Cad represents the concentration of the reagent in the reagent bottle.

[0035] In step S104, the current volume of carryover remaining in the reservoir and fluid flow path is updated based on the first concentration, the second concentration, and the volume of the reagent bottle.

[0036] In the process of filling a reagent into a container and drawing the reagent out of the container through a fluid flow path, the volume of carryover remaining in the container and in the fluid flow path may vary.

[0037] In some embodiments, after one round of filling the container with reagent and withdrawing the reagent from the container via the liquid flow path, the current volume of carryover remaining in the container and the liquid flow path is replaced with an updated carryover volume determined as follows: Then, during the next round of filling the container with reagent and withdrawing the reagent from the container via the liquid flow path, the concentration of the reagent can be monitored in real time based on the updated carryover volume.

[0038] The updated carryover volume is determined based on the reagent concentration measured during the draw, the reagent concentration measured during the fill, and the volume of the reagent bottle. For example, the updated carryover volume is: Formula: Vco' = (CDF - CDD) / CDF×Vb where Vco' represents the updated carryover volume, CDD represents the reagent concentration measured during the draw, CDF represents the reagent concentration measured during the fill, and Vb is Container capacity Represents.

[0039] The above formula is Formula: CDD = ((Vco×CW) + CDF×(Vb-Vco)) ÷Vb Here, CW represents the water concentration. When CW=0, CDD = (CDF × (Vb-Vco)) ÷ Vb, from which the above formula is derived.

[0040] In step S105, if the third concentration is less than the preset limit concentration, the user is notified to replace the current reagent and / or the current reagent is refreshed with a reagent of a higher concentration.

[0041] The reagents used for tissue processing need to be adapted to a certain concentration level, for example, the concentration of the reagent should be higher than a preset limit concentration, otherwise the tissue processing cannot be realized correctly.

[0042] To ensure the effectiveness of tissue processing, if a reagent does not comply with a certain concentration level, the user may be notified to replace the reagent with a reagent having a concentration higher than a preset limit concentration, or the reagent may be refreshed with a reagent of a higher concentration.

[0043] The user may be notified in a variety of ways, for example via an audio, video, vibration or other signal.

[0044] The method for monitoring the concentration of a reagent can be applied to several scenarios, for example, the method can be applied to tissue processing. During tissue processing, a tissue dehydrator is an important device for realizing tissue dehydration, while a reagent is required during tissue dehydration. According to the method for monitoring the concentration of a reagent, the tissue dehydrator can monitor the concentration of a reagent used for tissue dehydration in real time, so as to notify a user to replace the reagent in a timely manner, i.e., on the one hand, to ensure that the concentration of the reagent meets the requirements of tissue dehydration, and on the other hand, to avoid reagent waste.

[0045] According to the method for monitoring the concentration of a reagent, a first concentration of the current reagent can be obtained during filling of a current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path, a current volume of carryover remaining in the container and the liquid flow path determined in a process of filling a previous reagent into the container and aspirating the previous reagent from the container can be obtained, a second concentration of the current reagent can be obtained during aspirating of the current reagent from the container into the reagent bottle through the liquid flow path can be obtained, a third concentration of the current reagent after aspirating the reagent from the container into the reagent bottle can be determined based on the preset concentration, the volume of the reagent bottle and the current volume of carryover can be updated based on the first concentration, the second concentration and the volume of the reagent bottle, and a user is notified to replace the current reagent if the third concentration is less than the preset limit concentration. In this manner, the concentration of the reagent can be accurately determined in real time, and the volume of carryover remaining in the container can be dynamically updated so that a user can be notified to replace the reagent at an appropriate time, thereby improving the quality of tissue processing.

[0046] FIG. 2 is a flow chart illustrating a process for obtaining the concentration of a reagent according to some embodiments of the present disclosure.

[0047] In step S201, all required reagents, each having a predetermined concentration, are prepared.

[0048] In step S202, a protocol is selected to run.

[0049] The protocol indicates how to use reagents in tissue processing, for example, the order in which to load and aspirate reagents into and out of containers from reagent bottles via liquid flow paths and from containers to reagent bottles, or similar matters.

[0050] In step S203, algorithm parameters are initialized.

[0051] For example, the current volumes of carryover remaining in the containers and liquid flow paths are initialized to 0 (zero), and the volumes of the reagent bottles are allocated according to the actual situation. The algorithm parameters can be stored in a cache.

[0052] In step S204, reagents are loaded into the containers via the fluid flow paths according to the selected protocol.

[0053] In step S205, during the filling of the reagent, the concentration of the reagent during the filling is measured by a concentration meter.

[0054] In step S206, it is determined whether the reagent has been loaded into the container.

[0055] In step S207, the concentration of the reagent in the container is calculated.

[0056] The concentration of the reagent in the container is Formula:Vaf = (Vb - Vco)×Cbf; Formula: Caf = Vaf÷Vb; It is calculated by using where Vaf represents the volume of the main component of the reagent in the container, Vco represents the current volume of carryover, and Vb is Container capacity where Cbf represents the predetermined concentration and Caf represents the concentration of the reagent in the vessel.

[0057] In the first round of the protocol, the current volume of carryover has an initial value, but in subsequent rounds of the protocol, the current volume of carryover has a value determined in the round immediately preceding the current round. For example, in the first round of the protocol, a value of the volume of carryover is determined, and in the second round of the protocol, the value of the volume of carryover determined in the first round is used to calculate the concentration of a reagent.

[0058] In step S208, texture processing is performed.

[0059] In step S209, the reagent is drawn from the container through the liquid flow path into the reagent bottle.

[0060] In step S210, during the aspirating of the reagent, the concentration of the reagent during the aspirating is measured by a concentration meter.

[0061] In step S211, it is determined whether reagent has been aspirated from the container.

[0062] After the reagent is drawn from the container into the reagent bottle, the reagent bottle is not filled (to the brim) due to carryover remaining in the container and in the liquid flow path, and the reagent bottle is then replenished with a reagent having a predetermined concentration so that the amount of reagent reaches the capacity of the reagent bottle.

[0063] In step S212, the current concentration of the reagent in the reagent bottle is calculated.

[0064] The current concentration of the reagent in the reagent bottle is Formula: Vad = (Vb - Vco) ×Caf + Vco×Cbf; Formula: Cad = Vad÷Vb; It is calculated by using where Vad represents the volume of the main component of the reagent in the reagent bottle, Vco represents the current volume of the carryover, and Vb is Container capacity where Cbf represents the predetermined concentration, Caf represents the concentration of the reagent in the container, and Cad represents the current concentration of the reagent in the reagent bottle.

[0065] In step S213, the current volume of carryover remaining in the vessel and the liquid flow path is updated.

[0066] The current volume of carryover remaining in the container is Formula: Vco' = (CDF - CDD) / CDF×Vb; is updated using where CDD represents the concentration of the reagent during the draw, CDF represents the concentration of the reagent during the fill, and Vb is Container capacity and Vco' represents the updated carryover volume.

[0067] In step S214 it is determined whether the protocol has been completely executed, if yes the process is terminated, otherwise step S215 is executed.

[0068] In step S215, the next reagent is selected for tissue processing.

[0069] In the process of obtaining the concentration of the reagent, the volume of carryover remaining in the container is dynamically adjusted each round so that a more accurate concentration of the reagent can be obtained.

[0070] The embodiments of the present disclosure further provide an apparatus for monitoring the concentration of a reagent, as shown in Figure 3, the apparatus includes a first acquisition module 301, a second acquisition module 302, a determination module 303, an update module 304, and a notification module 305 and / or a refresh module 306.

[0071] The first acquisition module 301 is configured to acquire a first concentration of a current reagent measured during filling of a current reagent having a preset concentration from a reagent bottle into a container via a liquid flow path.

[0072] The second acquisition module 302 is configured to acquire the current volume of carryover remaining in the container and the liquid flow path, determined during the process of filling and withdrawing the previous reagent into and from the container.

[0073] The determination module 303 is configured to obtain a second concentration of the current reagent measured during the aspirating of the current reagent from the container to the reagent bottle via the liquid flow path, and to determine a third concentration of the current reagent after the aspirating of the current reagent from the container to the reagent bottle based on the preset concentration, the capacity of the reagent bottle, and the current volume of carryover.

[0074] The update module 304 is configured to update the current volume of carryover remaining in the container and the fluid flow path based on the first concentration, the second concentration, and the volume of the reagent bottle.

[0075] The notification module 305 is configured to notify a user to replace the current reagent if the third concentration is less than a preset limit concentration.

[0076] The refresh module 306 is configured to refresh the current reagent with a higher concentration of the reagent.

[0077] The device for monitoring the concentration of a reagent can be used to obtain a first concentration of the current reagent measured during filling of a current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path, and a current volume of carryover remaining in the container and liquid flow path determined in a process of filling a previous reagent into the container and aspirating the previous reagent from the container, and then a second concentration of the current reagent measured during aspirating of the current reagent from the container into the reagent bottle through the liquid flow path, and a third concentration of the current reagent after the reagent is aspirated from the container into the reagent bottle based on the preset concentration, the volume of the reagent bottle and the current volume of carryover can be determined, and the current volume of carryover remaining in the container and liquid flow path can be updated based on the first concentration, the second concentration and the volume of the reagent bottle, and a user is notified to replace the current reagent if the third concentration is less than the preset limit concentration. In this manner, the volume of carryover remaining in the container can be dynamically updated so that the concentration of the reagent can be accurately determined in real time and a user is notified to replace the reagent at the appropriate time, thereby improving the quality of tissue processing.

[0078] In some embodiments, the determination module 303 includes a first determination unit, a control unit, and a second determination unit. The first determination unit is configured to determine a concentration of a current reagent in the container based on a preset concentration, a volume of the reagent bottle, and a current volume of carryover after the current reagent is filled into the container. The control unit is configured to control the reagent bottle such that a current reagent having a preset concentration is replenished after the current reagent is aspirated from the container to the reagent bottle. The second determination unit is configured to determine a third concentration of a current reagent in the reagent bottle based on a concentration of the current reagent in the container, a volume of the reagent bottle, a preset concentration, and a current volume of carryover.

[0079] In some embodiments, the first determination unit includes a first calculation sub-module and a second calculation sub-module. The first calculation sub-module is configured to calculate a volume of the major component of the reagent currently in the container using the formula: Vaf = (Vb - Vco) x Cbf. The second calculation sub-module is configured to calculate a concentration of the reagent currently in the container using the formula: Caf = Vaf÷Vb, where Vaf represents the volume of the major component of the reagent currently in the container, Vco represents the current volume of carryover, and Vb represents the concentration of the reagent currently in the container using the formula: Caf = Vaf÷Vb. Container capacity where Cbf represents the preset concentration and Caf represents the current concentration of the reagent in the container.

[0080] In some embodiments, the second determination unit includes a third calculation sub-module and a fourth calculation sub-module. The third calculation sub-module is configured to calculate a volume of the major component of the current reagent in the reagent bottle using the formula: Vad = (Vb - Vco) x Caf + Vco x Cbf. The fourth calculation sub-module is configured to calculate a third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb, where Vad represents the volume of the major component of the current reagent in the reagent bottle, Vco represents the current volume of carryover, and Vb represents the third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb. Container capacity , Cbf represents the preset concentration, Caf represents the current concentration of the reagent in the container, and Cad represents the third concentration.

[0081] In some embodiments, the update module includes a third determining unit, a fourth determining unit, and a replacing unit, wherein the third determining unit is configured to determine an updated carry-over volume using a formula: Vco' = (CDF - CDD) / CDF x Vb, where Vco' represents the updated carry-over volume, CDD represents the second concentration of the current reagent, CDF represents the first concentration of the current reagent, and Vb is the first concentration of the current reagent. Container capacity The replacement unit is configured to replace the current volume of the carry-over with the updated volume of the carry-over.

[0082] In some embodiments, the current reagent is selected from ethanol and xylene.

[0083] The specific manner(s) in which each module performs the processing is described in detail in the method embodiments of the present invention, and will not be described in detail here with respect to the device of the above embodiments.

[0084] The present disclosure also provides an equipment for monitoring the concentration of a reagent. The equipment may include a controller, a cache, a processor, and a concentration meter. The concentration meter is configured to measure the concentration of a reagent during filling and to measure the concentration of a second reagent during drawing. The controller is configured to control the reagent to be filled from the reagent bottle through the liquid flow path into the container and to control the reagent to be drawn from the container through the liquid flow path into the reagent bottle. In some embodiments, the controller may be further configured to control the reagent bottle to be refilled with a reagent having a preset concentration. The cache may be configured to cache parameters including the preset concentration, the moisture concentration, the volume of the reagent bottle, and the current volume of carryover remaining in the container. The processor may be configured to: obtain a concentration of the reagent measured by the concentration meter during filling of the reagent from the reagent bottle into the container, obtain the obtained parameters including a current volume of carryover remaining in the container, the volume of the reagent bottle, a moisture concentration, and a preset concentration from the cache, obtain a concentration of the reagent measured during drawing, determine a concentration of the reagent after the reagent is drawn from the container into the reagent bottle based on the preset concentration, the volume of the reagent bottle and the current volume of carryover, determine an updated current volume of carryover based on the concentration measured during filling, the concentration measured during drawing and the volume of the reagent bottle, and replace the current volume of carryover saved in the cache with the updated current volume of carryover. Further, the processor is configured to notify a user to replace the reagent and / or refresh the current reagent with a reagent of a higher concentration if the concentration of the reagent is below the preset limit concentration.

[0085] FIG. 4 is a schematic diagram of the internal structure of an electric device according to an embodiment of the present disclosure. As shown in FIG. 4, the electric device includes a processor and a memory connected by a system bus. The processor is used to provide computing and control the overall processing support capabilities of the electric device. The memory may include a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operation system and a computer program. The computer program is executed by the processor to perform the method of monitoring the concentration of a reagent of the embodiment of the present disclosure. The internal memory provides a cached processing environment for the processing system and the computer program in the non-volatile storage medium. The electric device may be a mobile phone, a tablet computer, a personal digital assistant, or a wearable device.

[0086] Each module in the device for monitoring the concentration of a reagent of the embodiment of the present disclosure may be implemented in the form of a computer program, which, when executed by a processor, performs the steps of the method of the embodiment of the present disclosure.

[0087] Embodiments of the present disclosure also provide a computer-readable storage medium including computer-executable instructions that, when executed by one or more processors, cause the one or more processors to perform the steps of the method for monitoring the concentration of a reagent of embodiments of the present disclosure.

[0088] A computer program product is provided that includes instructions that, when run on a computer, cause the computer to perform the method of monitoring the concentration of a reagent of an embodiment of the present disclosure.

[0089] References to memory, storage, database, or other media used in this application may include non-volatile and / or volatile memory. Suitable non-volatile memory may include read only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM), which is used as external cache memory. By way of example and not limitation, RAM is available in various forms such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDR SDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).

[0090] Although the preferred embodiments of the present disclosure have been described in detail above in combination with the accompanying drawings, the present disclosure is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present disclosure, various simple modifications can be made to the technical solutions of the present disclosure. All of these simple modifications belong to the protection scope of the present disclosure.

[0091] Furthermore, it should be noted that the specific technical features described in the above specific embodiments may be combined in any suitable manner without contradiction, and the various possible combinations will not be described further in the present disclosure in order to avoid unnecessary repetition.

[0092] Furthermore, unless inconsistent with the ideas of the present disclosure, various embodiments of the present disclosure may be combined in an intentional manner and should be considered as being disclosed in the present disclosure.

Claims

1. obtaining a first concentration of a current reagent measured during filling of the current reagent having a preset concentration from a reagent bottle into the container via a liquid flow path; Obtaining a current volume of carryover remaining in the container and in the liquid flow path, determined during the process of filling and withdrawing the previous reagent into and from the container; obtaining a second concentration of the current reagent measured during aspirating the current reagent from the container through the liquid flow path to the reagent bottle, and determining a third concentration of the current reagent after aspirating the current reagent from the container to the reagent bottle based on the preset concentration, the volume of the container, and a current volume of carryover; updating a current volume of carryover remaining in the container and the liquid flow path based on the first concentration, the second concentration and the volume of the container; and notifying a user to replace the current reagent and / or refreshing the current reagent with a reagent of a higher concentration if the third concentration is less than a preset limit concentration; 4. A computer-implemented method for monitoring a concentration of a reagent, comprising:

2. determining a third concentration of the current reagent after aspirating the current reagent from the container to a reagent bottle based on the preset concentration, the volume of the container, and a current volume of carryover; determining a concentration of a current reagent in the container based on the preset concentration, the volume of the container, and a current volume of carryover after the current reagent is filled into the container; Controlling the reagent bottle to be refilled with the current reagent having a preset concentration after the current reagent is drawn from the container into the reagent bottle; and determining a third concentration of the current reagent in the reagent bottle based on the current concentration of the reagent in the container, the volume of the reagent bottle, the preset concentration, and the current volume of carryover; 2. The method of claim 1, comprising:

3. determining a current concentration of the reagent in the container based on a preset concentration, the volume of the container, and a current volume of carryover; Calculating the volume of the major component of the reagent currently in the container using the formula: Vaf = (Vb - Vco) x Cbf; and Calculate the concentration of the current reagent in the container using the formula: Caf = Vaf ÷ Vb; Including, Vaf represents the volume of the main component of the reagent currently in the container, Vco represents the current volume of the carryover, Vb represents the volume of the container, and Cbf represents the preset concentration, Caf represents the concentration of the reagent currently in the container; The method of claim 2.

4. determining a third concentration of the current reagent in the reagent bottle based on the concentration of the current reagent in the container, the volume of the container, the preset concentration, and the current volume of carryover; Calculating the volume of the major component of the current reagent in the reagent bottle using the formula: Vad = (Vb' - Vco) × Caf + Vco × Cbf; and Calculating the third concentration of the current reagent in the reagent bottle using the formula: Cad = Vad÷Vb'; Including, Vad represents the volume of the main component of the reagent currently in the reagent bottle, Vco represents the current volume of the carryover, Vb' represents the capacity of the reagent bottle, Cbf represents the preset concentration, Caf represents the concentration of the current reagent in the container, and Cad represents the third concentration; The method of claim 2.

5. updating a current volume of carryover remaining in the container and the liquid flow path based on the first concentration, the second concentration and the volume of the container; Calculate the updated carryover volume using the formula: Vco' = (CDF - CDD) / CDF x Vb; replacing the current volume of carryover with the updated volume of carryover; Including, Vco' represents the updated carryover volume, CDD represents the second concentration of the current reagent, CDF represents the first concentration of the current reagent, and Vb represents the volume of the container. The method according to any one of claims 1 to 4.

6. The current reagent is selected from the group including ethanol and xylene; The method according to any one of claims 1 to 5.

7. a first acquisition module configured to acquire a first concentration of a current reagent measured during filling of the current reagent having a preset concentration from a reagent bottle into a container through a liquid flow path; a second acquisition module configured to acquire a current volume of carryover in the container and the liquid flow path, determined during a process of filling the container with a previous reagent and aspirating the previous reagent from the container; a determination module configured to obtain a second concentration of the current reagent measured during an aspirating of the current reagent from the container to the reagent bottle via the liquid flow path, and to determine a third concentration of the current reagent after the current reagent is aspirated from the container to the reagent bottle based on the preset concentration, the volume of the container, and a current volume of carryover; an update module configured to update a current volume of carryover in the container and the liquid flow path based on the first concentration, the second concentration, and a volume of the container; a notification module configured to notify a user to replace the current reagent when the third concentration is less than a preset limit concentration and / or a refresh module configured to refresh the current reagent with a reagent having a higher concentration; A device for monitoring the concentration of a reagent comprising:

8. The decision module is a first determining unit configured to determine a concentration of a current reagent in the container based on a preset concentration, a volume of the container, and a current volume of carryover after the current reagent is filled into the container; a control unit configured to control the current reagent to be drawn from the container to the reagent bottle after drawing the current reagent from the container to the reagent bottle and to control the reagent bottle to be replenished with the current reagent having a preset concentration; a second determining unit configured to determine a third concentration of the current reagent in the reagent bottle based on the concentration of the current reagent in the container, the volume of the reagent bottle, the preset concentration, and a current volume of carryover; Includes 8. The apparatus of claim 7.

9. a first decision unit, a first calculation sub-module configured to calculate the volume of the main component of the current reagent in the container using the formula: Vaf = (Vb - Vco) x Cbf; a second calculation sub-module configured to calculate the concentration of the current reagent in the container using the formula: Caf = Vaf÷Vb; Including, Vaf represents the volume of the main component of the reagent currently in the container, Vco represents the current volume of the carryover, Vb represents the volume of the container, and Cbf represents the preset concentration, Caf represents the concentration of the reagent currently in the container; 9. The apparatus of claim 8.

10. a second decision unit, a third calculation sub-module configured to calculate the volume of the main component of the current reagent in the reagent bottle using the formula: Vad = (Vb' - Vco) x Caf + Vco x Cbf; a fourth calculation sub-module configured to calculate a third concentration of the current reagent in the reagent bottle using the formula: Cad=Vad÷Vb'; Including, Vad represents the volume of the main component of the reagent currently in the reagent bottle, Vco represents the current volume of the carryover, Vb' represents the capacity of the reagent bottle, Cbf represents the preset concentration, Caf represents the concentration of the current reagent in the container, and Cad represents the third concentration; 9. The apparatus of claim 8.

11. The update module is a third determination unit configured to calculate an updated carryover volume using the formula: Vco' = (CDF - CDD) / CDF x Vb; a replacement unit configured to replace a current volume of the carryover with an updated volume of the carryover; Including, Vco' represents the updated carryover volume, CDD represents the second concentration of the current reagent, CDF represents the first concentration of the current reagent, and Vb represents the volume of the container. An apparatus according to any one of claims 7 to 10.

12. The device according to any one of claims 7 to 11, wherein the current reagent is selected from the group comprising ethanol and xylene.

13. A processor; a memory configured to store instructions executable by a processor; Including, The instructions, when executed by a processor, cause the processor to carry out the method according to any one of claims 1 to 6. Electrical devices.

14. A concentration meter, a cache, a controller and a processor, The controller is configured to control a current reagent having a preset concentration to be filled from the reagent bottle into the container through the liquid flow path, and to aspirate the current reagent from the container into the reagent bottle through the liquid flow path; a concentration meter configured to measure a first concentration of the current reagent during filling and a second concentration of the current reagent during drawing; a cache configured to cache parameters including a preset concentration, a volume of the container, a current volume of carryover in the container and the liquid flow path, and a preset limit concentration; The processor: Obtaining a first concentration of a current reagent measured during filling; Retrieving from the cache the preset concentration, the moisture concentration, the volume of the vessel, the preset concentration limit, and the current volume of carryover remaining in the vessel; obtaining a second concentration of the current reagent measured during the draw; determining a third concentration of the current reagent after aspirating the current reagent from the container into a reagent bottle based on the preset concentration, the volume of the container, and the current volume of carryover; determining an updated current volume of carryover in the container and the liquid flow path based on the first concentration, the second concentration and the volume of the container, and replacing the current volume of carryover saved in the cache with the updated current volume of carryover; If the third concentration is less than a preset limit concentration, notifying a user to replace the current reagent and / or refreshing the current reagent with a reagent of a higher concentration; It is configured as follows: An instrument for monitoring the concentration of reagents.

15. A non-transitory computer readable medium having stored thereon instructions for causing a processor to carry out the steps of the method according to any one of claims 1 to 6.

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