Compounds and their uses

Abstract

The present disclosure features compounds useful for treating BAF complex-associated disorders.

Description

[Background technology]

[0001] The present invention relates to compounds useful for modulating the BRG1 or BRM-associated factor (BAF) complex. In particular, the present invention relates to compounds useful for treating disorders associated with BAF complex function.

[0002] Chromatin regulation is essential for gene expression, and ATP-dependent chromatin remodeling is the mechanism by which such gene expression occurs. The human switch / sucrose non-fermenting (SWI / SNF) chromatin remodeling complex, also known as the BAF complex, contains two SWI2-like ATPases known as BRG1 (Brahma-related gene-1) and BRM (Brahma). The transcriptional activator BRG1, also known as the ATP-dependent chromatin remodeler SMARCA4, is encoded by the SMARCA4 gene on chromosome 19. BRG1 is overexpressed in some cancer tumors and is required for cancer cell proliferation. BRM, also known as the likely global transcriptional activator SNF2L2 and / or the ATP-dependent chromatin remodeler SMARCA2, is encoded by the SMARCA2 gene on chromosome 9 and has been shown to be essential for tumor cell proliferation in cells characterized by loss-of-function mutations in BRG1. Deactivation of BRG and / or BRM leads to downstream effects in cells, including cell cycle arrest and tumor suppression. Summary of the Invention

[0003] The present invention features compounds useful for modulating the BAF complex. In some embodiments, the compounds are useful for treating disorders associated with alterations in the BAF complex, such as disorders associated with alterations in one or both of the BRG1 and BRM proteins. The compounds of the present invention can be used alone or in combination with other pharmaceutically active agents to treat such disorders.

[0004] In one aspect, the present invention provides a compound having the structure of Formula I, or a pharmaceutically acceptable salt thereof: [ka] During the ceremony, Ring system A is a 5- to 9-membered heterocyclyl or heteroaryl; m is 0, 1, 2, or 3; k is 0, 1, or 2; Each R 1 are independently halo, optionally substituted C-C alkyl, or optionally substituted C-C cycloalkyl (e.g., each R 1 is independently halo or optionally substituted C-C alkyl; R 2 is H or optionally substituted C1-C6 alkyl; each X is independently halo; L is a linker, The present invention features a compound or a pharmaceutically acceptable salt thereof, wherein B is a degrading moiety.

[0005] In some embodiments, the compound has the structure of formula IA: [ka] In the formula, the dashed bond represents a single bond or a double bond.

[0006] In some embodiments, the compound has the structure of formula IB. [ka]

[0007] In some embodiments, the compound has the structure of formula IC: [ka] In the formula, each R 1 is independently an optionally substituted C1-C6 alkyl.

[0008] In some embodiments, the compound has the structure of formula ID: [ka] In the formula, each R 1 is independently an optionally substituted C1-C6 alkyl.

[0009] In some embodiments, the compound has a structure of formula IE. [ka]

[0010] In some embodiments, the compound has the structure of formula IF. [ka]

[0011] In some embodiments, R 2 In some embodiments, m is 0.

[0012] In some embodiments, the compound has the structure of formula IG. [ka]

[0013] In some embodiments, the compound has the structure of formula IH: [ka]

[0014] In some embodiments, the decomposition moiety B has the structure of formula A-1: [ka] During the ceremony, Y 1 teeth, [ka] and RA5 is H, optionally substituted C1-C6 alkyl, or optionally substituted C1-C6 heteroalkyl; R A6 is H or optionally substituted C1-C6 alkyl, and R A7 is H or optionally substituted C1-C6 alkyl, or R A6 and R A7 each combine together with the carbon atom to which it is attached to form an optionally substituted C3-C6 carbocyclyl or an optionally substituted C2-C5 heterocyclyl, or R A6 and R A7 each combine together with the carbon atom to which it is attached to form an optionally substituted C3-C6 carbocyclyl or an optionally substituted C2-C5 heterocyclyl; R A8 is H, optionally substituted C1-C6 alkyl, or optionally substituted C1-C6 heteroalkyl; R A1 , R A2 , R A3 , and R A4 Each of the following may be independently H, A 2 , halogen, optionally substituted C1-C6 alkyl, optionally substituted C1-C6 heteroalkyl, optionally substituted C3-C 10 Carbocyclyl, optionally substituted C2-C9 heterocyclyl, optionally substituted C6-C 10 aryl, optionally substituted C2-C9 heteroaryl, optionally substituted C2-C6 alkenyl, optionally substituted C2-C6 heteroalkenyl, optionally substituted —O—C3-C6 carbocyclyl, hydroxyl, thiol, or optionally substituted amino, or R A1 and R A2 , R A2 and R A3 , and / or R A3 and R A4 combine with the carbon atoms to which they are attached, [ka] Forming [ka] is an optionally substituted C6-C 10 Aryl, optionally substituted C-C 10 carbocyclyl, optionally substituted C2-C9 heteroaryl, or C2-C9 heterocyclyl, any of which is optionally represented by A 2 is replaced by R A1 , R A2 , R A3 , and R A4 One of them is A 2 or [ka] But, A 2 is replaced by A 2 is the bond between the degradation moiety and the linker.

[0015] In some embodiments, R A5 is H or methyl. In some embodiments, R A5 is H.

[0016] In some embodiments, R A1 , R A2 , R A3 , and R A4 each independently represents H or A 2 is.

[0017] In some embodiments, R A1 is A 2 and R A2 , R A3 , and R A4 Each of is H.

[0018] In some embodiments, R A2 is A 2 and R A1 , R A3, and R A4 Each of is H.

[0019] In some embodiments, R A3 is A 2 and R A1 , R A2 , and R A4 Each of is H.

[0020] In some embodiments, R A4 is A 2 and R A1 , R A2 , and R A3 Each of is H.

[0021] In some embodiments, Y 1 teeth, [ka] is.

[0022] In some embodiments, R A6 is H. In some embodiments, R A7 is H.

[0023] In some embodiments, Y 1 teeth, [ka] is.

[0024] In some embodiments, R A8 is H or optionally substituted C1-C6 alkyl. In some embodiments, R A8 is H or methyl. In some embodiments, R A8 is methyl.

[0025] In some embodiments, the degrading moiety comprises a structure of formula A2. [ka]

[0026] In some embodiments, the decomposition moiety is [ka] is.

[0027] In some embodiments, the degrading moiety comprises a structure of formula A4. [ka]

[0028] In some embodiments, the degradation moiety is [ka] is.

[0029] In some embodiments, the degrading moiety comprises a structure of formula A5. [ka]

[0030] In some embodiments, the decomposition moiety comprises the structure of formula A6. [ka]

[0031] In some embodiments, the degrading moiety comprises the structure of formula A8. [ka]

[0032] In some embodiments, the decomposition moiety comprises a structure of formula A10. [ka]

[0033] In some embodiments, the degradation moiety is [ka] Includes the structure of

[0034] In some embodiments, the degradation moiety is [ka] Includes the structure of

[0035] In some embodiments, the degrading moiety has the structure of formula C: [ka] During the ceremony, L 4 But -N(R B1 )(R B2 ), [ka] and R B1 But, H, A 2 , optionally substituted C1-C6 alkyl, or optionally substituted C1-C6 heteroalkyl; R B2 is H, optionally substituted C1-C6 alkyl, or optionally substituted C1-C6 heteroalkyl; R B3 But, A 2 , optionally substituted C1-C6 alkyl, optionally substituted C1-C6 heteroalkyl, optionally substituted C3-C 10 Carbocyclyl, optionally substituted C6-C 10 aryl, optionally substituted C1-C6 alkyl C3-C 10 Carbocyclyl or optionally substituted C1-C6 alkylC6-C 10 is aryl, R B4 is H, optionally substituted C1-C6 alkyl, optionally substituted C3-C 10 Carbocyclyl, optionally substituted C6-C10 aryl, optionally substituted C1-C6 alkyl C3-C 10 Carbocyclyl or optionally substituted C1-C6 alkylC6-C 10 is aryl, R B5 is H, optionally substituted C1-C6 alkyl, or optionally substituted C1-C6 heteroalkyl; v2 is 0, 1, 2, 3, or 4; Each R B6 But independently, A 2 , halogen, optionally substituted C1-C6 alkyl, optionally substituted C1-C6 heteroalkyl, optionally substituted C3-C 10 Carbocyclyl, optionally substituted C2-C9 heterocyclyl, optionally substituted C6-C 10 aryl, optionally substituted C2-C9 heteroaryl, optionally substituted C2-C6 alkenyl, optionally substituted C2-C6 heteroalkenyl, hydroxy, thiol, or optionally substituted amino; R B7 and R B8 each independently represents H, halogen, optionally substituted C-C alkyl, or optionally substituted C-C 10 is aryl, R B9 is H or optionally substituted C1-C6 alkyl; A 2 is the bond between the degradation moiety and the linker, R B1 , R B3 , and R B6 Only one of the 2 The structure, or a pharmaceutically acceptable salt thereof.

[0036] In some embodiments, the degrading moiety has the structure of formula C1. [ka]

[0037] In some embodiments, the degradation moiety is: [ka]

[0038] In some embodiments, the degradation moiety is: [ka]

[0039] In some embodiments, the degradation moiety is: [ka]

[0040] In some embodiments, the degrading moiety has the structure of formula C2. [ka]

[0041] In some embodiments, R B9 is an optionally substituted C1-C6 alkyl. In some embodiments, R B9 is methyl.

[0042] In some embodiments, R B9 is attached to the (S)-steric center.

[0043] In some embodiments, the degradation moiety is: [ka]

[0044] In some embodiments, the linker has the structure of Formula II A 1 -(B 1 ) f -(C 1 ) g-(B 2 ) h -(D)-(B 3 ) i -(C 2 ) j -(B 4 ) k -A 2 Formula II or a pharmaceutically acceptable salt thereof, During the ceremony, A 1 is the bond between the linker and ring system A, A 2 is the bond between the degradation moiety and the linker, B 1 , B 2 , B 3 , and B 4 each independently represents an optionally substituted C1-C4 alkyl, an optionally substituted C6-C 10 Aryl, optionally substituted C-C 10 Aryl C 1-4 alkyl, optionally substituted C1-C4 heteroalkyl, optionally substituted C3-C 10 Cycloalkyl, optionally substituted C-C heterocyclyl, optionally substituted C 6-12 Aryl, O, S, S(O)2, or NR N and Each R N are independently H, optionally substituted C 1-4 Alkyl, optionally substituted C 2-4 Alkenyl, optionally substituted C 2-4 Alkynyl, optionally substituted C 2-6 Heterocyclyl, optionally substituted C 2-6 Heteroaryl or optionally substituted C 1-7 is heteroalkyl, C 1 and C 2 each is independently carbonyl, thiocarbonyl, sulfonyl, or phosphoryl; each of f, g, h, i, j, and k is independently 0 or 1; D is an optionally substituted C 1-10 Alkyl, optionally substituted C 2-10 Alkenyl, optionally substituted C 2-10 Alkynyl, optionally substituted C 2-6 Heterocyclyl, optionally substituted C 2-6 Heteroaryl, optionally substituted C 6-12 Aryl, optionally substituted C-C 10 Polyethylene glycol or optionally substituted C 1-10 Heteroalkyl, or A 1 -(B 1 ) f -(C 1 ) g -(B 2 ) h -(B 3 ) i -(C 2 ) j -(B 4 ) k -A 2 It is a chemical bond that bonds to

[0045] In some embodiments, B 1 , B 2 , B 3 , and B 4 each independently represents an optionally substituted C1-C4 alkyl, an optionally substituted C6-C 10 Aryl, optionally substituted C-C 10 Aryl C 1-4 alkyl, optionally substituted C1-C4 heteroalkyl, optionally substituted C3-C 10 cycloalkyl, optionally substituted C2-C6 heterocyclyl, O, S, S(O)2, or NR N and D is optionally substituted C 1-10 Alkyl, optionally substituted C 2-10 Alkenyl, optionally substituted C 2-10 Alkynyl, optionally substituted C 2-6 Heterocyclyl, optionally substituted C 6-12 Aryl, optionally substituted C-C 10Polyethylene glycol or optionally substituted C 1-10 Heteroalkyl, or A 1 -(B 1 ) f -(C 1 ) g -(B 2 ) h -(B 3 ) i -(C 2 ) j -(B 4 ) k -A 2 It is a chemical bond that connects

[0046] In some embodiments, B 1 , B 2 , B 3 , and B 4 each independently represents an optionally substituted C1-C2 alkyl, an optionally substituted C1-C3 heteroalkyl, an optionally substituted C2-C6 heterocyclyl, or NR N is.

[0047] In some embodiments, each R N is independently H or optionally substituted C1-C4 alkyl.

[0048] In some embodiments, each R N are independently H or CH3.

[0049] In some embodiments, B 1 and B 4 each of which is independently: [ka]

[0050] In some embodiments, B 1 and B 4 each of which is independently: [ka]

[0051] In some embodiments, B 1 teeth, [ka] is.

[0052] In some embodiments, B 1 teeth, [ka] is.

[0053] In some embodiments, B 4 teeth, [ka] is.

[0054] In some embodiments, B 4 teeth, [ka] is.

[0055] In some embodiments, C 1 and C 2 Each of the [ka] is.

[0056] In some embodiments, C 1 teeth, [ka] is.

[0057] In some embodiments, C 2 teeth, [ka] is.

[0058] In some embodiments, B 2 is optionally substituted C1-C4 alkyl. In some embodiments, B 2 is an optionally substituted C2-C6 heterocyclyl.

[0059] In some embodiments, B 2 teeth, [ka] is.

[0060] In some embodiments, D is an optionally substituted C-C 10 It is alkyl.

[0061] In some embodiments, f is 1. In some embodiments, g is 0. In some embodiments, g is 1. In some embodiments, h is 0. In some embodiments, h is 1. In some embodiments, i is 0. In some embodiments, i is 1. In some embodiments, j is 0. In some embodiments, j is 1. In some embodiments, k is 0. In some embodiments, k is 1.

[0062] In some embodiments, the linker is [ka] [ka] [ka] [ka] It has the following structure.

[0063] In some embodiments, the linker is [ka] [ka] It has the following structure.

[0064] In some embodiments, the shortest chain of atoms connecting the two valences of the linker is 2 to 10 atoms in length.

[0065] In some embodiments, the shortest chain of atoms connecting the two valences of the linker is six atoms in length.

[0066] In some embodiments, the linker is [ka] [ka] It has the following structure.

[0067] In some embodiments, the linker is [ka] [ka] It has the following structure.

[0068] In some embodiments, the linker is any one of compounds 1-310 in Table 1 (e.g., BRG1 IC 50 and BRM IC 50 In some embodiments, the linker has the structure of any one of compounds 1-310 in Table 1 (e.g., BRM IC 50In some embodiments, the linker has the structure of any one of compounds 1-310 in Table 1 (e.g., BRM IC 50 is ++ or higher (e.g., +++ or ++++ (e.g., ++++)), and BRG1 IC 50 and BRM IC 50 and the ratio of 1 to 1 is at least 5 (e.g., at least 7, 10, 15, 20, 25, or 30).

[0069] In one aspect, the invention features a compound selected from the group consisting of 1-310 in Table 1, and pharmaceutically acceptable salts thereof. In some embodiments, the compound is a BRG1 IC 50 and BRM IC 50 In some embodiments, the compound is any one of compounds 1-310 in Table 1, or a pharmaceutically acceptable salt thereof, having a BRM IC ratio of at least 5 (e.g., at least 7, 10, 15, 20, 25, or 30). 50 In some embodiments, the compound is any one of compounds 1-310 in Table 1, or a pharmaceutically acceptable salt thereof, having a BRM IC of ++ or higher (e.g., +++ or ++++ (e.g., ++++)). In some embodiments, the compound is any one of compounds 1-310 in Table 1, or a pharmaceutically acceptable salt thereof, as seen in Table 19. 50 is ++ or higher (e.g., +++ or ++++ (e.g., ++++)), and BRG1 IC 50 and BRM IC 50 or a pharmaceutically acceptable salt thereof. [Table 1-1] [Table 1-2] [Table 1-3]

Table 1-4

Table 1-5

Table 1-6

Table 1-7

Table 1-8

Table 1-9

Table 1-10

Table 1-11

Table 1-12

Table 1-13

Table 1-14

Table 1-15

Table 1-16

Table 1-17

Table 1-18

Table 1-19

Table 1-20

Table 1-21

Table 1-22

Table 1-23

Table 1-24

Table 1-25

Table 1-26

Table 1-27

Table 1-28

Table 1-29

Table 1-30

Table 1-31

Table 1-32

Table 1-33

Table 1-34

Table 1-35

Table 1-36

Table 1-37

Table 1-38

Table 1-39

Table 1-40

Table 1-41

Table 1-42

Table 1-43

Table 1-44

Table 1-45

Table 1-46

Table 1-47

Table 1-48

Table 1-49

Table 1-50

Table 1-51

Table 1-52

Table 1-53

Table 1-54

Table 1-55

Table 1-56

Table 1-57

Table 1-58

Table 1-59

Table 1-60

Table 1-61

Table 1-62

Table 1-63

Table 1-64

Table 1-65

Table 1-66

Table 1-67

Table 1-68

Table 1-69

Table 1-70

Table 1-71

Table 1-72

Table 1-73

Table 1-74

Table 1-75

Table 1-76

Table 1-77

Table 1-78

Table 1-79

Table 1-80

Table 1-81

Table 1-82

Table 1-83

Table 1-84

Table 1-85

Table 1-86

Table 1-87

Table 1-89

Table 1-90

Table 1-91

Table 1-92

Table 1-93

Table 1-94

Table 1-95

Table 1-96

Table 1-97

Table 1-98

Table 1-99

Table 1-100

Table 1-101

Table 1-103

Table 1-104

Table 1-105

[0070] In the table above, the upper double dashed bond indicates a π-aromatic bond.

[0071] In some embodiments, the compound is50 and BRM IC 50 to a BRG1 IC of at least 5. In some embodiments, the compound 50 and BRM IC 50 to a BRG1 IC of at least 7. In some embodiments, the compound 50 and BRM IC 50 to a BRG1 IC of at least 10. In some embodiments, the compound 50 and BRM IC 50 In some embodiments, the compound has a BRG1 IC 50 and BRM IC 50 In some embodiments, the compound has a BRG1 IC 50 and BRM IC 50 In some embodiments, the compound has a BRG1 IC 50 and BRM IC 50 The ratio of

[0072] In one aspect, the invention features a pharmaceutical composition including any of the aforementioned compounds and a pharmaceutically acceptable excipient.

[0073] In another aspect, the invention features a method for decreasing the activity of a BAF complex in a cell, the method comprising contacting the cell with an effective amount of any of the aforementioned compounds or a pharmaceutical composition thereof.

[0074] In some embodiments, the cells are cancer cells.

[0075] In another aspect, the invention features a method of treating a BAF complex-associated disorder in a subject in need thereof, the method including administering to the subject an effective amount of any of the aforementioned compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound) or a pharmaceutical composition thereof.

[0076] In some embodiments, the BAF complex-associated disorder is cancer.

[0077] In a further aspect, the invention features a method of inhibiting BRM, the method including contacting a cell with an effective amount of any of the foregoing compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound) or a pharmaceutical composition thereof.

[0078] In some embodiments, the cells are cancer cells.

[0079] In another aspect, the invention features a method of inhibiting BRG1, the method including contacting a cell with an effective amount of any of the aforementioned compounds or a pharmaceutical composition thereof.

[0080] In some embodiments, the cells are cancer cells.

[0081] In a further aspect, the invention features a method of inhibiting BRM and BRG1, the method comprising contacting a cell with an effective amount of any of the foregoing compounds or a pharmaceutical composition thereof.

[0082] In some embodiments, the cells are cancer cells.

[0083] In another aspect, the invention features a method of treating a disorder associated with a loss-of-function mutation in BRG1 in a subject in need thereof, the method including administering to the subject an effective amount of any of the foregoing compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound) or a pharmaceutical composition thereof.

[0084] In some embodiments, the disorder associated with BRG1 loss-of-function mutation is cancer. In other embodiments, the subject is determined to have a BRG1 loss-of-function disorder, for example, determined to have a BRG1 loss-of-function cancer (for example, determined to comprise cancer cells with BRG1 loss-of-function).

[0085] In another aspect, the invention features a method for inducing apoptosis in a cell, the method including contacting the cell with an effective amount of any of the aforementioned compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound) or a pharmaceutical composition thereof.

[0086] In some embodiments, the cells are cancer cells.

[0087] In a further aspect, the invention features a method of treating cancer in a subject in need thereof, the method including administering to the subject an effective amount of any of the foregoing compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound) or a pharmaceutical composition thereof.

[0088] In some embodiments of any of the aforementioned methods, the cancer is non-small cell lung cancer, colorectal cancer, bladder cancer, cancer of unknown primary site, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, esophagogastric cancer, pancreatic cancer, hepatobiliary cancer, soft tissue sarcoma, ovarian cancer, head and neck cancer, renal cell carcinoma, bone cancer, non-Hodgkin's lymphoma, small cell lung cancer, prostate cancer, embryonal tumors, germ cell tumors, cervical cancer, thyroid cancer, salivary gland cancer, gastrointestinal neuroendocrine tumors, uterine sarcoma, gastrointestinal stromal tumors, CNS cancer, thymic tumor, adrenocortical carcinoma, appendix cancer, small intestine cancer, or penile cancer.

[0089] In some embodiments of any of the aforementioned methods, the cancer is non-small cell lung cancer, colorectal cancer, bladder cancer, cancer of unknown primary, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, or penile cancer.

[0090] In some embodiments of any of the foregoing methods, the cancer is a drug-resistant cancer or has undergone prior therapy (e.g., vemurafenib, dacarbazine, CTLA4 inhibitors, PD1 inhibitors, interferon therapy, BRAF inhibitors, MEK inhibitors, radiation therapy, temozolomide, irinotecan, CAR-T therapy, Herceptin®, Perjeta®, tamoxifen, Xeloda®, docetaxel, platinum agents such as carboplatin, paclitaxel, and have not responded to prior chemotherapy or chemotherapy regimens (taxanes such as fluoxetine and docetaxel, ALK inhibitors, MET inhibitors, Alimta®, Abraxane®, Adriamycin®, gemcitabine, Avastin®, Halaven®, neratinib, PARP inhibitors, ARN810, mTOR inhibitors, topotecan, Gemzar®, VEGFR2 inhibitors, folate receptor antagonists, demcizumab, fosbretabulin, or PDL1 inhibitors).

[0091] In some embodiments of any of the aforementioned methods, the cancer has or has been determined to have a BRG1 mutation. In some embodiments of any of the aforementioned methods, the BRG1 mutation is homozygous. In some embodiments of any of the aforementioned methods, the cancer does not have or has been determined to not have an epidermal growth factor receptor (EGFR) mutation. In some embodiments of any of the aforementioned methods, the cancer does not have or has been determined to not have an anaplastic lymphoma kinase (ALK) driver mutation. In some embodiments of any of the aforementioned methods, the cancer has or has been determined to have a KRAS mutation. In some embodiments of any of the aforementioned methods, the BRG1 mutation is in the ATPase catalytic domain of the protein. In some embodiments of any of the aforementioned methods, the BRG1 mutation is a deletion at the C-terminus of BRG1.

[0092] In another aspect, the disclosure provides a method of treating a BAF-associated disorder (e.g., cancer or viral infection) in a subject in need thereof, the method comprising contacting a cell with an effective amount of any of the aforementioned compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound), or a pharmaceutically acceptable salt thereof, or any of the aforementioned pharmaceutical compositions. In some embodiments, the disorder is a viral infection caused by a virus selected from the Retroviridae family, such as lentiviruses (e.g., human immunodeficiency virus (HIV) and deltaretroviruses (e.g., human T-cell leukemia virus type I (HTLV-I)), human T-cell leukemia virus type II (HTLV-II)), Hepadnaviridae family (e.g., hepatitis B virus (HBV)), Flaviviridae family (e.g., hepatitis C virus (HCV)), Adenoviridae family (e.g., human adenovirus), Herpesviridae family (e.g., human cytomegalovirus (HCMV), Epstein-Barr virus, herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), human herpesvirus type 6 (HHV-6), herpesvirus K*, CMV, varicella-zoster virus), Papillomaviridae family (e.g., human papillomavirus (HPV), HPV E1), Parvoviridae (e.g., parvovirus B19), Polyomaviridae (e.g., JC virus and BK virus), Paramyxoviridae (e.g., measles virus), or Togaviridae (e.g., rubella virus). In some embodiments, the disorder is coffin schisis, neurofibromatosis (e.g., NF-1, NF-2, or schwannoma), or multiple meningiomas.

[0093] In another aspect, the present disclosure provides a method for treating a viral infection in a subject in need thereof, the method comprising administering to the subject an effective amount of any of the aforementioned compounds (e.g., a BRM / BRG1 dual inhibitor compound or a BRM-selective compound), or a pharmaceutically acceptable salt thereof, or any of the aforementioned pharmaceutical compositions. In some embodiments, the viral infection is caused by a virus selected from the Retroviridae family, such as lentivirus (e.g., human immunodeficiency virus (HIV) and deltaretrovirus (e.g., human T-cell leukemia virus type I (HTLV-I)), human T-cell leukemia virus type II (HTLV-II)), Hepadnaviridae family (e.g., hepatitis B virus (HBV)), Flaviviridae family (e.g., hepatitis C virus (HCV)), Adenoviridae family (e.g., human adenovirus), Herpesviridae family (e.g., human cytomegalovirus (HCMV), Epstein-Barr virus, herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), human herpesvirus type 6 (HHV-6), herpesvirus K*, CMV, varicella-zoster virus), Papillomaviridae family (e.g., human papillomavirus (HPV), HPV E1), Parvoviridae (e.g., parvovirus B19), Polyomaviridae (e.g., JC virus and BK virus), Paramyxoviridae (e.g., measles virus), or Togaviridae (e.g., rubella virus).

[0094] In some embodiments of any of the foregoing aspects, the compound is a BRM-selective compound. In some embodiments, the BRM-selective compound inhibits the level and / or activity of BRM to a degree that is at least 10 times greater than the compound inhibits the level and / or activity of BRG1, and / or the compound binds to BRM to a degree that is at least 10 times greater than the compound binds to BRG1. For example, in some embodiments, the BRM-selective compound has an IC 50 or IP 50 At least 10-fold lower IC50 or IP 50 In some embodiments of any of the foregoing aspects, the compound is a BRM / BRG1 dual inhibitor compound. In some embodiments, the BRM / BRG1 dual inhibitor compound has similar activity against both BRM and BRG1 (e.g., activity of the compound against BRM and BRG1 within 10-fold (e.g., less than 5-fold, less than 2-fold)). In some embodiments, the activity of the BRM / BRG1 dual inhibitor compound is greater against BRM. In some embodiments, the activity of the BRM / BRG1 dual inhibitor compound is greater against BRG1. For example, in some embodiments, the BRM / BRG1 dual inhibitor compound has an IC 50 or IP 50 However, IC against BRG1 50 or IP 50 It is within 10 times of

[0095] In another aspect, the invention features a method of treating melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, or hematological cancer in a subject in need thereof, the method comprising administering to the subject an effective amount of any of the aforementioned compounds or a pharmaceutical composition thereof.

[0096] In another aspect, the invention features a method of reducing melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, or hematological cancer tumor growth in a subject in need thereof, the method comprising administering to the subject an effective amount of any of the aforementioned compounds or a pharmaceutical composition thereof.

[0097] In another aspect, the invention features a method of inhibiting metastatic progression of melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, or hematological cancer in a subject, comprising administering an effective amount of any of the preceding compounds or a pharmaceutical composition thereof.

[0098] In another aspect, the invention features a method of inhibiting metastatic colonization of melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, or hematological cancer in a subject, the method comprising administering an effective amount of any of the foregoing compounds or a pharmaceutical composition thereof.

[0099] In another aspect, the invention features a method for reducing the level and / or activity of BRG1 and / or BRM in melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, blood cancer cells, or esophageal cancer cells, comprising contacting the cells with an effective amount of any of the foregoing compounds or a pharmaceutical composition thereof.

[0100] In some embodiments of any of the above aspects, the melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, or blood cells are in the subject.

[0101] In some embodiments of any of the above aspects, an effective amount of a compound reduces the level and / or activity of BRG1 by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) compared to a reference substance. In some embodiments, an effective amount of a compound reduces the level and / or activity of BRG1 by at least 50% (e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) compared to a reference substance. In some embodiments, an effective amount of a compound reduces the level and / or activity of BRG1 by at least 90% (e.g., 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%).

[0102] In some embodiments, an effective amount of a compound reduces BRG1 levels and / or activity by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) for at least 12 hours (e.g., 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, 24 hours, 30 hours, 36 hours, 48 hours, 72 hours, or more) compared to a reference substance. In some embodiments, an effective amount of a compound that reduces the level and / or activity of BRG1 by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) for at least 4 days (e.g., 5 days, 6 days, 7 days, 14 days, 28 days, or more) compared to a reference substance.

[0103] In some embodiments of any of the above aspects, an effective amount of compound reduces the level and / or activity of a BRM by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) compared to a reference substance. In some embodiments, an effective amount of compound reduces the level and / or activity of a BRM by at least 50% (e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) compared to a reference substance. In some embodiments, an effective amount of compound reduces the level and / or activity of a BRM by at least 90% (e.g., 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%).

[0104] In some embodiments, an effective amount of a compound reduces the level and / or activity of a BRM by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) for at least 12 hours (e.g., 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, 24 hours, 30 hours, 36 hours, 48 hours, 72 hours, or more) compared to a reference substance. In some embodiments, an effective amount of a compound that reduces the level and / or activity of a BRM by at least 5% (e.g., 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%) for at least 4 days (e.g., 5 days, 6 days, 7 days, 14 days, 28 days, or more) compared to a reference substance.

[0105] In some embodiments, the subject has cancer. In some embodiments, the cancer expresses BRG1 and / or BRM protein, and / or the cell or subject has been identified as expressing BRG1 and / or BRM. In some embodiments, the cancer expresses BRG1 protein, and / or the cell or subject has been identified as expressing BRG1. In some embodiments, the cancer expresses BRM protein, and / or the cell or subject has been identified as expressing BRM. In some embodiments, the cancer is melanoma (e.g., uveal melanoma, mucosal melanoma, or cutaneous melanoma). In some embodiments, the cancer is prostate cancer. In some embodiments, the cancer is a hematological cancer, such as multiple myeloma, large cell lymphoma, acute T-cell leukemia, acute myeloid leukemia, myelodysplastic syndrome, immunoglobulin A lambda myeloma, diffuse mixed histiocytic and lymphocytic lymphoma, B-cell lymphoma, acute lymphoblastic leukemia (e.g., T-cell acute lymphoblastic leukemia or B-cell acute lymphoblastic leukemia), diffuse large cell lymphoma, or non-Hodgkin's lymphoma. In some embodiments, the cancer is breast cancer (e.g., ER-positive breast cancer, ER-negative breast cancer, triple-positive breast cancer, or triple-negative breast cancer). In some embodiments, the cancer is bone cancer (e.g., Ewing's sarcoma). In some embodiments, the cancer is renal cell carcinoma (e.g., microphthalmia transcription factor (MITF) family translocation renal cell carcinoma (tRCC)). In some embodiments, the cancer is metastatic (e.g., the cancer has spread to the liver). A metastatic cancer may include cells that exhibit migratory cell migration and / or invasion, and / or may include cells that exhibit endothelial recruitment and / or angiogenesis. In other embodiments, the migratory cancer is a cell migration cancer. In yet other embodiments, the cell migration cancer is a non-metastatic cell migration cancer. A metastatic cancer may be a cancer that spreads via seeding the surfaces of the peritoneum, pleura, pericardium, or subarachnoid space. Alternatively, a metastatic cancer may be a cancer that spreads via the lymphatic system or hematogenously.In some embodiments, an effective amount of an agent that reduces the level and / or activity of BRG1 and / or BRM is an amount effective to inhibit metastatic colonization of a cancer to the liver.

[0106] In some embodiments, the cancer harbors a mutation in GNAQ. In some embodiments, the cancer harbors a mutation in GNA11. In some embodiments, the cancer harbors a mutation in PLCB4. In some embodiments, the cancer harbors a mutation in CYSLTR2. In some embodiments, the cancer harbors a mutation in BAP1. In some embodiments, the cancer harbors a mutation in SF3B1. In some embodiments, the cancer harbors a mutation in EIF1AX. In some embodiments, the cancer harbors a TFE3 translocation. In some embodiments, the cancer harbors a TFEB translocation. In some embodiments, the cancer harbors a MITF translocation. In some embodiments, the cancer harbors an EZH2 mutation. In some embodiments, the cancer harbors a SUZ12 mutation. In some embodiments, the cancer harbors an EED mutation.

[0107] In some embodiments, the method further comprises administering to the subject or contacting the cells with an anti-cancer therapy, e.g., a chemotherapeutic or cytotoxic agent, immunotherapy, surgery, radiation therapy, hyperthermia, or photocoagulation. In some embodiments, the anti-cancer therapy is a chemotherapeutic or cytotoxic agent, e.g., an antimetabolite, an antimitotic, an antitumor antibiotic, an asparagine-specific enzyme, a bisphosphonate, an anti-neoplastic agent, an alkylating agent, a DNA repair enzyme inhibitor, a histone deacetylase inhibitor, a corticosteroid, a demethylating agent, an immunomodulatory agent, a Janus-related kinase inhibitor, a phosphinositide 3-kinase inhibitor, a proteasome inhibitor, or a tyrosine kinase inhibitor.

[0108] In some embodiments, the compounds of the present invention are used in combination with another anti-cancer therapy used to treat uveal melanoma, such as surgery, a MEK inhibitor, and / or a PKC inhibitor. For example, in some embodiments, the method further comprises performing surgery before, after, or simultaneously with the administration of the compound of the present invention. In some embodiments, the method further comprises administering a MEK inhibitor and / or a PKC inhibitor before, after, or simultaneously with the administration of the compound of the present invention.

[0109] In some embodiments, the anti-cancer therapy and the compound of the invention are administered within 28 days of each other, and each in an amount together effective to treat the subject.

[0110] In some embodiments, the subject or cancer has and / or has been identified as having a loss-of-function mutation in BRG1.

[0111] In some embodiments, the cancer is resistant to one or more chemotherapeutic or cytotoxic agents (e.g., the cancer has been determined to be resistant to a chemotherapeutic or cytotoxic agent (e.g., by a genetic marker) or has been determined to be likely to be resistant to a chemotherapeutic or cytotoxic agent (e.g., a cancer that has failed to respond to a chemotherapeutic or cytotoxic agent)). In some embodiments, the cancer has failed to respond to one or more chemotherapeutic or cytotoxic agents. In some embodiments, the cancer is resistant to or has failed to respond to dacarbazine, temozolomide, cisplatin, treosulfan, fotemustine, IMCgp100, CTLA-4 inhibitors (e.g., ipilimumab), PD-1 inhibitors (e.g., nivolumab or pembrolizumab), PD-L1 inhibitors (e.g., atezolizumab, avelumab, or durvalumab), mitogen-activated protein kinase (MEK) inhibitors (e.g., selumetinib, binimetinib, or trametinib), and / or protein kinase C (PKC) inhibitors (e.g., sotrastaurin or IDE196).

[0112] In some embodiments, the cancer is resistant to or has not responded to previously administered therapeutic agents used to treat uveal melanoma, such as MEK inhibitors or PKC inhibitors. For example, in some embodiments, the cancer is resistant to or has not responded to mitogen-activated protein kinase (MEK) inhibitors (e.g., selumetinib, binimetinib, or tametinib) and / or protein kinase C (PKC) inhibitors (e.g., sotrastaurin or IDE196).

[0113] chemical terms The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting.

[0114] For any of the following chemical definitions, the number following an atomic symbol indicates the total number of atoms of that element present in a particular chemical moiety. As understood, other atoms, such as H atoms, or substituents described herein, may be present, as needed, to satisfy the valence of an atom. For example, an unsubstituted C alkyl group has the formula —CHCH. When used with groups defined herein, references to the number of carbon atoms include divalent carbons in acetal and ketal groups, but do not include the carbonyl carbon in acyl, ester, carbonate, or carbamate groups. References to the number of oxygen, nitrogen, or sulfur atoms in heteroaryl groups include only those atoms that form part of the heterocyclic ring.

[0115] The term "acyl," as used herein, represents an H or alkyl group, as defined herein, attached to the parent molecular group through a carbonyl group, and is exemplified by formyl (i.e., a carboxaldehyde group), acetyl, trifluoroacetyl, propionyl, and butanoyl. Exemplary unsubstituted acyl groups contain 1 to 6, 1 to 11, or 1 to 21 carbons.

[0116] As used herein, the term "alkyl" refers to a branched or straight-chain monovalent saturated aliphatic hydrocarbon radical of 1 to 20 carbon atoms (e.g., 1 to 16 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms, or 1 to 3 carbon atoms).

[0117] Alkylene is a divalent alkyl group. As used herein, the term "alkenyl," alone or in combination with other groups, refers to a straight-chain or branched hydrocarbon residue having a carbon-carbon double bond and having 2 to 20 carbon atoms (e.g., 2 to 16 carbon atoms, 2 to 10 carbon atoms, 2 to 6 carbon atoms, or 2 carbon atoms).

[0118] As used herein, the term "alkynyl," alone or in combination with other groups, refers to a straight-chain or branched hydrocarbon residue having a carbon-carbon triple bond and having 2 to 20 carbon atoms (e.g., 2 to 16 carbon atoms, 2 to 10 carbon atoms, 2 to 6 carbon atoms, or 2 carbon atoms).

[0119] As used herein, the term “amino” refers to —N(R N1 )2, and each R N1 are independently H, OH, NO2, N(R N2 )2, SO2OR N2 , SO2R N2 , SOR N2 , an N-protecting group, alkyl, alkoxy, aryl, arylalkyl, cycloalkyl, acyl (e.g., acetyl, trifluoroacetyl, or others described herein), and these enumerated R N1 Each of the groups may be optionally substituted, or two R N1 combine to form an alkylene or heteroalkylene, and each R N2 are independently H, alkyl, or aryl. The amino groups of the present invention can be unsubstituted amino (i.e., —NH) or substituted amino (i.e., —N(R N1 )2).

[0120] As used herein, the term "aryl" refers to an aromatic mono- or polycarbocyclic radical of 6 to 12 carbon atoms having at least one aromatic ring. Examples of such groups include, but are not limited to, phenyl, naphthyl, 1,2,3,4-tetrahydronaphthyl, 1,2-dihydronaphthyl, indanyl, and 1H-indenyl.

[0121] As used herein, the term "arylalkyl" refers to an alkyl group substituted with an aryl group. Exemplary unsubstituted arylalkyl groups are those having 7 to 30 carbons (e.g., C-C alkyl, C-C alkyl), such as benzyl and phenethyl. 10 Aryl, C1-C 10 Alkyl C6-C 10 Aryl, or C1-C 20 Alkyl C6-C 10 aryl, etc.) In some embodiments, alkyl and aryl can each be further substituted with 1, 2, 3, or 4 substituents as defined herein for each group.

[0122] As used herein, the term "azido" refers to an -N3 group.

[0123] As used herein, the term "bridged cyclyl" refers to a bridged polycyclic group of 5 to 20 carbons containing 1 to 3 bridges.

[0124] As used herein, the term "cyano" refers to a -CN group.

[0125] As used herein, the term "carbocyclyl" refers to a non-aromatic C-C ring in which the ring is formed by carbon atoms. 12 It refers to a monocyclic, bicyclic, or tricyclic structure. The carbocyclyl structure includes a cycloalkyl group and an unsaturated carbocyclyl radical.

[0126] As used herein, the term "cycloalkyl" refers to a saturated non-aromatic monovalent mono- or polycarbocyclic radical of 3 to 10, preferably 3 to 6, carbon atoms. This term is further exemplified by radicals such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, norbornyl, and adamantyl.

[0127] As used herein, the term "halo" means a fluorine (fluoro), chlorine (chloro), bromine (bromo), or iodine (iodo) radical.

[0128] As used herein, the term "heteroalkyl" refers to an alkyl group, as defined herein, in which one or more of the constituent carbon atoms has been replaced with nitrogen, oxygen, or sulfur. In some embodiments, a heteroalkyl group can be further substituted with one, two, three, or four substituents, as described herein for alkyl groups. An example of a heteroalkyl group is "alkoxy," which, as used herein, refers to alkyl-O- (e.g., methoxy and ethoxy). Heteroalkylene is a divalent heteroalkyl group. As used herein, the term "heteroalkenyl" refers to an alkenyl group, as defined herein, in which one or more of the constituent carbon atoms has been replaced with nitrogen, oxygen, or sulfur. In some embodiments, a heteroalkenyl group can be further substituted with one, two, three, or four substituents, as described herein for alkenyl groups. An example of a heteroalkenyl group is "alkenoxy," which, as used herein, refers to alkenyl-O-. Heteroalkenylene is a divalent heteroalkenyl group. As used herein, the term "heteroalkynyl" refers to an alkynyl group, as defined herein, in which one or more of the constituent carbon atoms has been replaced with nitrogen, oxygen, or sulfur. In some embodiments, heteroalkynyl groups can be further substituted with 1, 2, 3, or 4 substituents, as described herein for alkynyl groups. An example of a heteroalkynyl group is "alkynoxy," which, as used herein, refers to alkynyl-O-. Heteroalkynylene is a divalent heteroalkynyl group.

[0129] As used herein, the term "heteroaryl" refers to a monocyclic or polycyclic radical of 5 to 12 atoms having at least one aromatic ring and containing one, two, or three ring atoms selected from nitrogen, oxygen, and sulfur, with the remaining ring atoms being carbon. One or two ring carbon atoms of a heteroaryl group may be replaced by a carbonyl group. Examples of heteroaryl groups are pyridyl, pyrazolyl, benzoxazolyl, benzimidazolyl, benzothiazolyl, imidazolyl, oxaxolyl, and thiazolyl.

[0130] As used herein, the term "heteroarylalkyl" refers to an alkyl group substituted with a heteroaryl group. Exemplary unsubstituted heteroarylalkyl groups include those having 7 to 30 carbons (e.g., C1-C6 alkyl, C2-C9 heteroaryl, C1-C 10 alkyl C2-C9 heteroaryl, or C1-C 20 alkyl, C2-C9, 7-16 or 7-20 carbons, such as heteroaryl. In some embodiments, alkyl and heteroaryl can each be further substituted with 1, 2, 3, or 4 substituents as defined herein for each group.

[0131] As used herein, the term "heterocyclyl" refers to a monocyclic or polycyclic radical having 3 to 12 atoms having at least one ring containing 1, 2, 3, or 4 ring atoms selected from N, O, or S, and the ring is not aromatic. Examples of heterocyclyl groups include, but are not limited to, morpholinyl, thiomorpholinyl, furyl, piperazinyl, piperidinyl, pyranyl, pyrrolidinyl, tetrahydropyranyl, tetrahydrofuranyl, and 1,3-dioxanyl.

[0132] As used herein, the term "heterocyclylalkyl" refers to an alkyl group substituted with a heterocyclyl group. Exemplary unsubstituted heterocyclylalkyl groups include those having 7 to 30 carbons (e.g., C1-C6 alkyl, C2-C9 heterocyclyl, C1-C 10alkyl C2-C9 heterocyclyl, or C1-C 20 and alkyl (7-16 or 7-20 carbons, such as C2-C9 heterocyclyl). In some embodiments, alkyl and heterocyclyl can each be further substituted with 1, 2, 3, or 4 substituents, as defined herein for each group.

[0133] As used herein, the term "hydroxyalkyl" refers to an alkyl group substituted with an --OH group.

[0134] As used herein, the term "hydroxyl" refers to an --OH group.

[0135] As used herein, the term "N-protecting group" refers to a group intended to protect an amino group against undesired reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in Greene, "Protective Groups in Organic Synthesis," 3rd Edition (John Wiley & Sons, New York, 1999).N-protecting groups include, but are not limited to, acyl, aryloyl, or carbamyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, α-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and chiral auxiliaries such as protected or unprotected D,L, or D,L-amino acids such as alanine, leucine, and phenylalanine; sulfonyl-containing groups such as benzenesulfonyl and p-toluenesulfonyl; benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-20 dimethoxybenzyl Oxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, 1-(p-biphenylyl)-1-methylethoxycarbonyl, α,α-dimethyl 3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl Examples of N-protecting groups include carbamate-forming groups such as aryl, allyloxycarbonyl, 2,2,2-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitrophenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, and phenylthiocarbonyl, arylalkyl groups such as benzyl, triphenylmethyl, and benzyloxymethyl, and silyl groups such as trimethylsilyl. Preferred N-protecting groups are alloc, formyl, acetyl, benzoyl, pivaloyl, t-butylacetyl, alanyl, phenylsulfonyl, benzyl, t-butyloxycarbonyl (Boc), and benzyloxycarbonyl (Cbz).

[0136] As used herein, the term "nitro" refers to the group --NO.sub.2.

[0137] As used herein, the term "thiol" refers to a -SH group.

[0138] Alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl (e.g., cycloalkyl), aryl, heteroaryl, and heterocyclyl groups can be substituted or unsubstituted. If substituted, there will typically be 1 to 4 substituents, unless otherwise specified. Substituents include, for example, alkyl (e.g., unsubstituted and substituted, where the substituents include any group described herein, e.g., aryl, halo, hydroxy), aryl (e.g., substituted and unsubstituted phenyl), carbocyclyl (e.g., substituted and unsubstituted cycloalkyl), halo (e.g., fluoro), hydroxyl, heteroalkyl (e.g., substituted and unsubstituted methoxy, ethoxy, or thioalkoxy), heteroaryl, heterocyclyl, amino (e.g., NH or mono- or dialkylamino), azido, cyano, nitro, or thiol. Another exemplary substituent is oxo. For example, a carbonyl group is a carbon (e.g., an alkyl carbon, alkenyl carbon, alkynyl carbon, heteroalkyl carbon, heteroalkenyl carbon, heteroalkynyl carbon, carbocyclyl carbon, etc.) substituted with oxo. Alternatively, sulfur may be substituted with one or two oxo groups (e.g., —SO— or —SO— in a substituted heteroalkyl, heteroalkenyl, heteroalkynyl, or heterocyclyl group). Aryl, carbocyclyl (e.g., cycloalkyl), heteroaryl, and heterocyclyl groups may also be substituted with alkyl (unsubstituted and substituted, e.g., arylalkyl (e.g., substituted and unsubstituted benzyl)). In some embodiments, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, and heteroalkynyl are optionally substituted with 1, 2, 3, 4, or 5 substituents independently selected from the group consisting of aryl (e.g., substituted and unsubstituted phenyl), carbocyclyl (e.g., substituted and unsubstituted cycloalkyl), halo (e.g., fluoro), hydroxyl, heteroaryl, heterocyclyl, amino (e.g., NH or mono- or dialkylamino), azido, cyano, nitro, thiol, and oxo. In some embodiments, the substituents are themselves unsubstituted.

[0139] The compounds of the present invention may have one or more asymmetric carbon atoms and may exist in the form of optically pure enantiomers, mixtures of enantiomers such as racemates, optically pure diastereomers, mixtures of diastereomers, diastereomeric racemates, or mixtures of diastereomeric racemates. Optically active forms can be obtained, for example, by resolution of racemates, asymmetric synthesis, or asymmetric chromatography (chromatography using a chiral adsorbent or eluent). Thus, certain disclosed compounds may exist in various stereoisomeric forms. Stereoisomers are compounds that differ only in their spatial arrangement. Enantiomers are most commonly pairs of stereoisomers whose mirror images are not superimposable because they contain asymmetrically substituted carbon atoms that function as chiral centers. An enantiomer refers to one of a pair of molecules that are mirror images of each other and are not superimposable. Diastereomers are most commonly stereoisomers that are not related as mirror images because they contain two or more asymmetrically substituted carbon atoms and represent the configuration of substituents around one or more chiral carbon atoms. Enantiomers of a compound can be prepared, for example, by separating an enantiomer from a racemate using one or more well-known techniques and methods, such as chiral chromatography and separation methods based thereon. Suitable techniques and / or methods for separating enantiomers of the compounds described herein from a racemic mixture can be readily determined by one of ordinary skill in the art. A "racemate" or "racemic mixture" refers to a compound containing two enantiomers; such mixtures do not exhibit optical activity, i.e., they do not rotate the plane of polarized light. A "geometric isomer" refers to isomers that differ in the orientation of substituent atoms relative to a carbon-carbon double bond, a cycloalkyl ring, or a bridged bicyclic system. Atoms (other than H) on each side of a carbon-carbon double bond can be in the E (substituents are on opposite sides of the carbon-carbon double bond) or Z (substituents are oriented on the same side) configuration. "R," "S," "S*," "R*," "E," "Z," "cis," and "trans" refer to configurations relative to the core molecule. Certain disclosed compounds may exist in atropisomeric forms.Atropisomers are stereoisomers resulting from hindered rotation around a single bond, where the steric strain barrier to rotation is high enough to allow for the isolation of the conformers. The compounds of the present invention can be prepared as individual isomers by isomer-specific synthesis or by resolution from an isomeric mixture. Traditional resolution techniques include forming a salt of the free base of each isomer of the isomeric pair using an optically active acid (followed by fractional crystallization and regeneration of the free base), forming a salt of the acid form of each isomer of the isomeric pair using an optically active amine (followed by fractional crystallization and regeneration of the free acid), forming an ester or amide of each isomer of the isomeric pair using an optically pure acid, amine, or alcohol (followed by chromatographic separation and removal of the chiral auxiliary), or resolving an isomeric mixture of either the starting material or the final product using a variety of well-known chromatographic methods. When the stereochemistry of a disclosed compound is named or depicted by structure, the named or depicted stereoisomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% pure by weight relative to the other stereoisomers. When a single enantiomer is named or depicted by structure, the depicted or named enantiomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% optically pure by weight. When a single diastereomer is named or depicted by structure, the depicted or named diastereomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% pure by weight. Percent optical purity is the weight of the enantiomer, or the ratio of the weight of the enantiomer to the weight of its optical isomer. Diastereomeric purity by weight is the ratio of the weight of one diastereomer to the weight of all diastereomers. When the stereochemistry of a disclosed compound is named or shown by structure, the named or shown stereoisomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% pure by mole fraction relative to the other stereoisomers.When a single enantiomer is named or depicted by structure, the depicted or named enantiomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% pure by mole fraction. When a single diastereomer is named or depicted by structure, the depicted or named diastereomer is at least 60%, 70%, 80%, 90%, 99%, or 99.9% pure by mole fraction. Percent purity by mole fraction is the ratio of moles of enantiomer, or moles of enantiomer to moles of its optical isomer. Similarly, percent purity by mole fraction is the ratio of moles of diastereomer, or moles of diastereomer to moles of its isomer. When a disclosed compound is named or depicted by structure without indicating stereochemistry, and the compound has at least one chiral center, the name or structure should be understood to encompass any enantiomer of the compound free of the corresponding optical isomer, a racemic mixture of the compound, a mixture of the compound, or a mixture enriched in one enantiomer with respect to its corresponding optical isomer. When a disclosed compound is named or depicted by structure without indicating stereochemistry, and the compound has two or more chiral centers, the name or structure should be understood to encompass any diastereomer free of the other diastereomer, any diastereomer free of other diastereomeric pairs, mixtures of diastereomers, mixtures of diastereomeric pairs, mixtures of diastereomers enriched in one diastereomer with respect to the other, or mixtures of diastereomers enriched in one or more diastereomers with respect to the other. The present invention encompasses all of these forms.

[0140] The compounds of the present disclosure also include all isotopes of atoms present in the intermediate or final compounds. "Isotopes" refer to atoms having the same atomic number but different mass numbers resulting from different numbers of neutrons in the nucleus. For example, isotopes of hydrogen include tritium and deuterium.

[0141] Unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. Exemplary isotopes that can be incorporated into the compounds of the invention include: 2 H, 3 H, 11 C. 13 C. 14 C. 13 N, 15 N, 15 O. 17 O. 18 O. 32 P, 33 P, 35 S, 18 F, 36 Cl, 123 I, and 125 Isotopically labeled compounds (e.g., 3 H and 14 C) can be useful in compound or substrate tissue distribution assays. 3 H) and carbon-14 (i.e., 14 C) isotopes can be useful for their ease of preparation and detectability. Additionally, deuterium (i.e., 2 Substitution with heavier isotopes, such as H, may afford certain therapeutic benefits (e.g., increased in vivo half-life or reduced dosage requirements) due to greater metabolic stability. In some embodiments, one or more hydrogen atoms are 2 H or 3 H or one or more carbon atoms are replaced by 13 C or 14 replaced by C-enriched carbon. 15 O. 13 N, 11 C, and 18Positron-emitting isotopes, such as F, are useful in positron emission tomography (PET) studies to examine substrate receptor occupancy. Preparation of isotopically labeled compounds is known to those skilled in the art. For example, isotopically labeled compounds can generally be prepared by following procedures similar to those disclosed for the compounds of the present invention described herein, substituting a non-isotopically labeled reagent for an isotopically labeled reagent. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Methods and materials for use in this disclosure are described herein. Other suitable methods and materials known in the art may also be used. These materials, methods, and examples are illustrative only and are not intended to be limiting. All publications, patent applications, patents, sequences, database entries, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control.

[0142] definition In this application, unless otherwise clear from the context, (i) the term "a" may be understood to mean "at least one," (ii) the term "or" may be understood to mean "and / or," and (iii) the terms "comprising" and "including" may be understood to encompass the listed elements or steps, whether presented by themselves or together with one or more additional elements or steps.

[0143] As used herein, the terms "about" and "approximately" refer to values within 10% above and below the stated value. For example, the term "about 5 nM" indicates a range of 4.5 to 5.5 nM.

[0144] As used herein, the term "administration" refers to the administration of a composition (e.g., a compound or a preparation comprising a compound as described herein) to a subject or system. Administration to an animal subject (e.g., to a human) can be by any suitable route. For example, in some embodiments, administration can be bronchial (including by bronchial infusion), buccal, enteral, interdermal, intraarterial, intradermal, intragastric, intramedullary, intramuscular, intranasal, intraperitoneal, intrathecal, intratumoral, intravenous, intraventricular, mucosal, nasal, oral, rectal, subcutaneous, sublingual, topical, tracheal (including by intratracheal infusion), transdermal, vaginal, and intravitreal.

[0145] As used herein, the term "BAF complex" refers to the BRG1 or HRBM-associated factor complex in human cells.

[0146] As used herein, the term "BAF complex-associated disorder" refers to a disorder caused by or affected by the level of activity of the BAF complex.

[0147] As used herein, the term "BRG1 loss-of-function mutation" refers to a mutation in BRG1 that results in a protein with reduced activity (e.g., at least a 1% reduction in BRG1 activity, e.g., a 2%, 5%, 10%, 25%, 50%, or 100% reduction in BRG1 activity). Exemplary BRG1 loss-of-function mutations include, but are not limited to, homozygous BRG1 mutations and deletions at the C-terminus of BRG1.

[0148] As used herein, the term "BRG1 loss-of-function disorder" refers to a disorder (e.g., cancer) that exhibits a reduction in BRG1 activity (e.g., at least a 1% reduction in BRG1 activity, e.g., a 2%, 5%, 10%, 25%, 50%, or 100% reduction in BRG1 activity).

[0149] The term "cancer" refers to conditions caused by the proliferation of malignant cells, such as tumors, neoplasms, carcinomas, sarcomas, leukemias, and lymphomas.

[0150] As used herein, "combination therapy" or "administered in combination" means that two (or more) different agents or treatments are administered to a subject as part of a defined treatment regimen for a particular disease or condition. The treatment regimen defines the dosage and periodicity of administration of each agent so that the effects of the separate agents on the subject overlap. In some embodiments, delivery of two or more agents may be simultaneous or concurrent, or the agents may be co-formulated. In some embodiments, two or more agents are not co-formulated but are administered in a sequential manner as part of a prescribed regimen. In some embodiments, the administration of two or more agents or combined treatments is such that the reduction in symptoms or other parameters associated with the disorder is greater than that observed with one agent or treatment delivered alone or in the absence of the other. The effect of the two treatments may be partially additive, fully additive, or greater than additive (e.g., synergistic). The sequential or substantially simultaneous administration of each therapeutic agent can occur by any suitable route, including, but not limited to, oral, intravenous, intramuscular, and direct absorption through mucosal tissue. The therapeutic agents can be administered by the same route or by different routes, for example, a first therapeutic agent of the combination may be administered by intravenous injection, while a second therapeutic agent of the combination may be administered orally.

[0151] "Determining the level" of protein or RNA refers to detecting the protein or RNA, either directly or indirectly, by methods known in the art. "Directly determining" means performing a process to obtain a physical entity or value (e.g., performing an assay or test on a sample, or "analyzing a sample," as that term is defined herein). "Indirectly determining" refers to receiving a physical entity or value from another party or source (e.g., a third-party laboratory that directly obtained the physical entity or value). Methods for measuring protein levels generally include, but are not limited to, Western blotting, immunoblotting, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), immunoprecipitation, immunofluorescence, surface plasmon resonance, chemiluminescence, fluorescence polarization, phosphorescence, immunohistochemistry, matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF) mass spectrometry, liquid chromatography (LC) mass spectrometry, microcytometry, microscopy, fluorescence-activated cell sorting (FACS), and flow cytometry, as well as assays based on protein properties, including, but not limited to, enzymatic activity or interactions with other protein partners. Methods for measuring RNA levels are known in the art and include, but are not limited to, quantitative polymerase chain reaction (qPCR) and Northern blot analysis.

[0152] "Reducing the activity of the BAF complex" means reducing the level of activity associated with the BAF complex or an associated downstream effect. A non-limiting example of reducing the activity of the BAF complex is activation of Sox2. The activity level of the BAF complex can be measured using any method known in the art, for example, the method described in Kadoch et al. Cell, 2013, 153, 71-85, which is incorporated herein by reference.

[0153] As used herein, the term "degrading agent" refers to a small molecule compound that contains a degrading moiety, and the compound interacts with a protein (e.g., BRG1 and / or BRM) in a manner that results in degradation of the protein (e.g., binding of the compound results in at least a 5% reduction in the levels of the protein, e.g., in a cell or subject).

[0154] As used herein, the term "degradation moiety" refers to a moiety that, upon binding, results in degradation of a protein (e.g., BRG1 and / or BRM). In one example, the moiety binds to a protease or ubiquitin ligase that metabolizes the protein (e.g., BRG1 and / or BRM).

[0155] "Modulating the activity of a BAF complex" means altering the level of activity associated with a BAF complex (e.g., GBAF) or an associated downstream effect. The activity level of a BAF complex can be measured using any method known in the art, for example, the method described in Kadoch et al., Cell 153:71-85 (2013), which is incorporated herein by reference.

[0156] "Reducing the activity of BRG1 and / or BRM" means reducing the level of BRG1 and / or BRM-associated activity or associated downstream effect. A non-limiting example of inhibiting BRG1 and / or BRM activity is reducing the level of the BAF complex in a cell. The activity level of BRG1 and / or BRM can be measured using any method known in the art. In some embodiments, the agent that reduces BRG1 and / or BRM activity is a small molecule BRG1 and / or BRM degrader.

[0157] "Reducing the level of BRG1 and / or BRM" means decreasing the level of BRG1 and / or BRM in a cell or a subject. The level of BRG1 and / or BRM can be measured using any method known in the art.

[0158] By "level" is meant the level of a protein or mRNA encoding a protein, as compared to a reference material. A reference material can be any useful reference material, as defined herein. A "decreased level" or "increased level" of a protein means a decrease or increase in protein level as compared to a reference material (e.g., about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 100%, about 150%, about 200%, about 300%, about 400%, about 500% or more decrease or increase, as compared to a reference material). (e.g., a decrease or increase of more than about 10%, about 15%, about 20%, about 50%, about 75%, about 100%, or about 200%; a decrease or increase of less than about 0.01-fold, about 0.02-fold, about 0.1-fold, about 0.3-fold, about 0.5-fold, about 0.8-fold, or less; or an increase of about 1.2-fold, about 1.4-fold, about 1.5-fold, about 1.8-fold, about 2.0-fold, about 3.0-fold, about 3.5-fold, about 4.5-fold, about 5.0-fold, about 10-fold, about 15-fold, about 20-fold, about 30-fold, about 40-fold, about 50-fold, about 100-fold, about 1000-fold, or more). Protein levels may be expressed as mass / volume (e.g., g / dL, mg / mL, μg / mL, ng / mL) or as a percentage of total protein or mRNA in the sample.

[0159] As used herein, the term "inhibiting BRM" refers to blocking or reducing the level or activity of the ATPase catalytic binding domain or bromodomain of a protein. BRM inhibition can be determined using methods known in the art, such as a BRM ATPase assay, a Nano DSF assay, or a BRM luciferase cellular assay.

[0160] As used herein, the term "pharmaceutical composition" refers to a composition comprising a compound described herein, formulated with a pharmaceutically acceptable excipient and suitable for administration to a mammal, e.g., a human. Typically, a pharmaceutical composition is manufactured or sold, with the approval of a government regulatory agency, as part of a therapeutic regimen for the treatment of a mammalian disease. A pharmaceutical composition can be formulated, for example, for oral administration in a unit dosage form (e.g., a tablet, capsule, caplet, gelcap, or syrup), for topical administration (e.g., as a cream, gel, lotion, or ointment), for intravenous administration (e.g., as a sterile solution free of particulate embolic agents and in a solvent system suitable for intravenous use), or in any other pharmaceutically acceptable formulation.

[0161] As used herein, "pharmaceutically acceptable excipient" refers to any component of a compound described herein (e.g., a vehicle capable of suspending or dissolving an active compound) that has the properties of being substantially non-toxic and non-inflammatory in a patient. Excipients may include, for example, anti-adherents, antioxidants, binders, coatings, compression aids, disintegrants, dyes (colors), emollients, emulsifiers, fillers (diluents), film-forming or coating agents, flavors, fragrances, glidants (glidants), lubricants, preservatives, printing inks, adsorbents, suspending or dispersing agents, sweeteners, and water for hydration.

[0162] As used herein, the term "pharmaceutically acceptable salt" refers to any pharmaceutically acceptable salt of a compound, for example, any compound of Formula I or II. Pharmaceutically acceptable salts of any of the compounds described herein may include salts that are suitable for use in contact with the tissues of humans and animals without undue toxicity, irritation, or allergic response, and are within the scope of sound medical judgment and commensurate with a reasonable benefit / risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, pharmaceutically acceptable salts are described in Berge et al., J. Pharmaceutical Sciences 66:1-19, 1977 and Pharmaceutical Salts: Properties, Selection, and Use, (Eds. P.H. Stahl and C.G. Wermuth), Wiley-VCH, 2008. Salts can be prepared in situ during the final isolation and purification of the compounds described herein, or separately by reacting the free base group with a suitable organic acid.

[0163] The compounds of the present invention may have ionizable groups so that they can be prepared as pharmaceutically acceptable salts. These salts may be acid addition salts, including inorganic or organic acids, or salts may be prepared from inorganic or organic bases when the compounds of the present invention are in acidic form. Frequently, the compounds are prepared or used as pharmaceutically acceptable salts prepared as addition products of pharmaceutically acceptable acids or bases. Suitable pharmaceutically acceptable acids and bases, as well as methods for preparing suitable salts, are well known in the art. Salts may be prepared from pharmaceutically acceptable non-toxic acids and bases, including inorganic and organic acids and bases.

[0164] "Reference material" refers to any useful reference material used to compare protein or RNA levels. A reference material can be any sample, standard, standard curve, or level used for comparison purposes. A reference material can be a normal reference sample or reference standard or level. A "reference sample" can be, for example, a control, e.g., a predetermined negative control value such as a "normal control," or a previous sample taken from the same subject; a sample from a normal healthy subject, such as normal cells or normal tissue; a sample (e.g., cell or tissue) from a subject without a disease; a sample from a subject diagnosed with a disease but not yet treated with a compound of the present invention; a sample from a subject being treated with a compound of the present invention; or a sample of purified protein or RNA (e.g., any of those described herein) with a known normal concentration. A "reference standard or level" refers to a value or numerical value derived from a reference sample. A "normal control value" is a predetermined value indicative of a non-disease state, e.g., a value expected in a healthy control subject. Typically, a normal control value is expressed as a range ("between X and Y"), a high threshold ("below X"), or a low threshold ("above X"). A subject having a measured value within the normal control value for a particular biomarker is typically referred to as being "within the normal range" for that biomarker. A normal reference standard or level can be a value or number derived from a normal subject who does not have a disease or disorder (e.g., cancer); a subject who has been treated with a compound of the invention. In preferred embodiments, the reference sample, standard, or level is matched to the sample subject sample by at least one of the following criteria: age, weight, sex, stage of disease, and overall health. A standard curve of purified protein or RNA levels within the normal reference range, such as any of those described herein, can also be used as a reference.

[0165] As used herein, the term "subject" refers to any organism to which a composition according to the invention can be administered, for example, for experimental, diagnostic, prophylactic, and / or therapeutic purposes. Typical subjects include any animal (e.g., mammals such as mice, rats, rabbits, non-human primates, and humans). A subject may be a human or animal seeking or in need of treatment, requesting treatment, undergoing treatment, will undergo treatment in the future, or being treated by a trained professional for a particular disease or condition.

[0166] As used herein, the terms "treat," "treated," or "treating" refer to therapeutic treatment or any procedure where the purpose is to slow (alleviate) an undesirable physiological condition, disorder, or disease, or to obtain a beneficial or desired clinical result. Beneficial or desired clinical results include, but are not limited to, alleviation of symptoms; attenuation of the severity of the condition, disorder, or disease; stabilization (i.e., non-worsening) of the condition, disorder, or disease; delay in the onset or slowing of progression of the condition, disorder, or disease; improvement or remission (partial or complete) of the condition, disorder, or disease state; improvement in at least one measurable physical parameter not necessarily discernible by the patient; or enhancement or amelioration of the condition, disorder, or disease. Treatment includes eliciting a clinically significant response without excessive levels of side effects. Treatment also includes prolonging survival compared to expected survival if not receiving treatment. The compounds of the invention may also be used to "prophylactically treat" or "prevent" disorders, for example, in subjects at increased risk of developing the disorder.

[0167] The details of one or more embodiments of the invention are set forth in the description below. Other features, objects, and advantages of the invention will be apparent from the description and from the claims. DETAILED DESCRIPTION OF THE INVENTION

[0168] The present disclosure features compounds useful for inhibiting BRG1 and optionally BRM. These compounds may be used to modulate the activity of the BAF complex, for example, for the treatment of BAF-associated disorders (e.g., loss-of-function disorders of BRG1), such as cancer. Exemplary compounds described herein include compounds having a structure according to Formula I, or a pharmaceutically acceptable salt thereof. Formula I: [ka] During the ceremony, Ring system A is a 5- to 9-membered heterocyclyl or heteroaryl; m is 0, 1, 2, or 3; k is 0, 1, or 2; Each R 1 are independently halo, optionally substituted C-C alkyl, or optionally substituted C-C cycloalkyl (e.g., each R 1 is independently halo or optionally substituted C-C alkyl; R 2 is H or optionally substituted C1-C6 alkyl; each X is independently halo; L is a linker, B is the decomposition part.

[0169] In some embodiments, the compound has the structure of any one of compounds 1-310 in Table 1, or a pharmaceutically acceptable salt thereof.

[0170] Other embodiments, and exemplary methods for synthesis of the production of these compounds, are described herein.

[0171] The compounds described herein can be prepared, for example, using the representative compounds shown in Table 2. The compounds in Table 2 contain binding moieties for targeting BRG1 and / or BRM. [Table 2-1] [Table 2-2] [Table 2-3] [Table 2-4] [Table 2-5] [Table 2-6] [Table 2-7] [Table 2-8]

[0172] Pharmaceutical use The compounds described herein are useful in the methods of the invention and, without being bound by theory, are believed to exert their ability to modulate the level, status, and / or activity of the BAF complex, i.e., by inhibiting the activity of BRG1 and / or BRM proteins within the mammalian BAF complex. BAF complex-associated disorders include, but are not limited to, disorders associated with loss-of-function mutations in BRG1.

[0173] One aspect of the invention relates to a method of treating a disorder associated with a loss-of-function mutation in BRG1, such as cancer (e.g., non-small cell lung cancer, colon cancer, bladder cancer, cancer of unknown primary site, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, or penile cancer) in a subject in need thereof. In some embodiments, the compound is administered in an amount and for a time effective to result in one or more (e.g., two or more, three or more, four or more) of: (a) a reduction in tumor size; (b) a reduction in tumor growth rate; (c) an increase in tumor cell death; (d) a reduction in tumor progression; (e) a reduction in the number of metastases; (f) a reduction in the rate of metastasis; (g) a reduction in tumor recurrence; (h) an increase in the subject's survival rate; or (i) an increase in the subject's progression-free survival.

[0174] Treating cancer can result in a reduction in tumor size or volume. For example, after treatment, tumor size is reduced by 5% or more (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or more) relative to its size before treatment. Tumor size may be measured by any reproducible means of measurement. For example, tumor size may be measured as the diameter of the tumor.

[0175] Treating cancer may also result in a reduction in tumor number. For example, after treatment, tumor number is reduced by 5% or more (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or more) compared to the number before treatment. The number of tumors may be measured by any reproducible means of measurement; for example, the number of tumors may be measured by counting tumors visible to the naked eye or at a particular magnification (e.g., 2x, 3x, 4x, 5x, 10x, or 50x).

[0176] Treatment of cancer can result in a reduction in the number of metastatic nodules in other tissues or organs distant from the primary tumor site. For example, after treatment, the number of metastatic nodules is reduced by 5% or more (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or more) compared to the number before treatment. The number of metastatic nodules may be measured by any reproducible means of measurement. For example, the number of metastatic nodules may be measured by counting metastatic nodules visible to the naked eye or at a particular magnification (e.g., 2x, 10x, or 50x).

[0177] Treating cancer may result in an increase in the average survival time of a population of subjects treated according to the present invention compared to a population of untreated subjects. For example, the average survival time is increased by more than 30 days (more than 60 days, 90 days, or 120 days). The increase in average survival time of a population may be measured by any reproducible means. The increase in average survival time of a population may be measured, for example, by calculating the average length of survival for a population after the start of treatment with a compound of the present invention. The increase in average survival time of a population may also be measured, for example, by calculating the average length of survival for a population after the completion of the first round of treatment with a pharmaceutically acceptable salt of a compound of the present invention.

[0178] Treating cancer may also result in a reduction in mortality in a population of treated subjects compared to an untreated population. For example, mortality is reduced by more than 2% (e.g., more than 5%, 10%, or 25%). The reduction in mortality in a population of treated subjects may be measured by any reproducible means, for example, by calculating the average number of disease-related deaths per unit time for the population after initiation of treatment with a pharmaceutically acceptable salt of the present invention. The reduction in mortality in a population may also be measured, for example, by calculating the average number of disease-related deaths per unit time for the population after completion of the first round of treatment with a pharmaceutically acceptable salt of the present invention.

[0179] Exemplary cancers that may be treated by the present invention include, but are not limited to, non-small cell lung cancer, small cell lung cancer, colon cancer, bladder cancer, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, esophagogastric cancer, pancreatic cancer, hepatobiliary cancer, soft tissue sarcoma, ovarian cancer, head and neck cancer, renal cell carcinoma, bone cancer, non-Hodgkin's lymphoma, prostate cancer, embryonal tumors, germ cell tumors, cervical cancer, thyroid cancer, salivary gland cancer, gastrointestinal neuroendocrine tumors, uterine sarcoma, gastrointestinal stromal tumors, CNS cancer, thymic tumor, adrenocortical carcinoma, appendix cancer, small intestine cancer, and penile cancer.

[0180] Combination preparations and their uses The compounds of the invention can be combined with one or more therapeutic agents. In particular, the therapeutic agents can be those that treat or prophylactically treat any of the cancers described herein.

[0181] Combination therapy The compounds of the present invention can be used alone or in combination with additional therapeutic agents, such as other drugs for treating cancer or related symptoms, or in combination with other types of treatment for cancer. In combination therapy, the dosage of one or more therapeutic compounds may be reduced from the standard dosage when administered alone. For example, dosages may be empirically determined from drug combinations and permutations, or estimated by isobolographic analysis (e.g., Black et al., Neurology 65:S3-S6, 2005). In this case, the dosage of the combined compounds should provide a therapeutic effect.

[0182] In some embodiments, the second therapeutic agent is a chemotherapeutic agent (e.g., a cytotoxic agent or other compound useful in the treatment of cancer). These include alkylating agents, antimetabolites, folic acid analogs, pyrimidine analogs, purine analogs and related inhibitors, vinca alkaloids, epipodophyllotoxins, antibiotics, L-asparaginase, topoisomerase inhibitors, interferons, platinum coordination complexes, anthracenedione-substituted ureas, methylhydrazine derivatives, adrenocorticosteroids, progestins, estrogens, antiestrogens, androgens, antiandrogens, and gonadotropin-releasing hormone analogs. Also included are 5-fluorouracil (5-FU), leucovorin (LV), irenotecan, oxaliplatin, capecitabine, paclitaxel, and doxetaxel. Non-limiting examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclosphosphamide; alkylsulfonates such as busulfan, improsulfan, and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines, including altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphoramide, and trimethylolmelamine; acetogenins (especially bullatacin and bullatacinone); camptothecin (including the synthetic analog topotecan); bryostatin; kallistatin; CC-1065 (including its adozelesin, carzelesin, and cryptophycins (especially cryptophycin 1 and cryptophycin 8); dolastatins; duocarmycins (including synthetic analogs, KW-2189 and CB1-TM1); eleutherobin; pancratistatin; sarcodictin; spongistatins; nitrogen mustards such as chlorambucil, chlornaphazine, chlorophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, nobembitine, phenesterine, prednimustine, trofosfamide, and uracil mustard; nitrosoureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimnustine;Antibiotics such as enediyne antibiotics (e.g., calicheamicins, particularly calicheamicin gamma and calicheamicin omegal (see, e.g., Agnew, Chem. Intl. Ed Engl. 33:183-186 (1994)); dynemicins, including dynemicin A; bisphosphonates such as clodronate; esperamicin; and neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores), aclacinomycin, actinomycin, autramycin, azaserine, bleomycin, cactinomycin, carabicin, caminomycin, carzinophilin, chromomycin, dactinomycin, daunorubicin, detorubicin, leucine, 6-diazo-5-oxo-L-norleucine, Adriamycin® (doxorubicin including morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin, and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcelomycin, mitomycins such as mitomycin C, mycophenolic acid, nogalamycin, olivomycin, peplomycin, potfilomycin, puromycin, chelamycin, rodorubicin, streptomycin, Ptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; antimetabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogues such as fludarabine, 6-mercaptopurine, thiamiprine, and thioguanine; ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, and floxacin Pyrimidine analogues such as lysine; androgens such as calucelone, drostanolone propionate, epitiostanol, mepitiostane, and testolactone; antiadrenal drugs such as aminoglutethimide, mitotane, and trilostane; folic acid supplements such as furoic acid; aceglatone; aldophosphamide glycosides; aminolevulinic acid; eniluracil; amsacrine; bestravcil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elfomitine; elliptinium acetate; epothilones;Etoglucide; Gallium nitrate; Hydroxyurea; Lentinan; Lonidynin; Maytansinoids such as maytansine and ansamitocin; Mitoguazone; Mitoxantrone; Mopidanmol; Nitraerin; Pentostatin; Fenamet; Pirarubicin; Rosoxantrone; Podophyllic acid; 2-Ethylhydrazide; Procarbazine; PSK® Polysaccharide Complex (JHS Natural Products, Eugene, Oreg.); razoxane; rhizoxin; schizofuran; spirogermanium; tenuazonic acid; triaziquone; 2,2',2''-trichlorotriethylamine; trichothecenes (especially T-2 toxin, veracrine A, roridin A, and anguidine); urethane; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside ("Ara-C"); cyclophosphamide; thiotepa; taxoids, such as Taxol® paclitaxel (Bristol-Myers Squibb Oncology, Princeton, NJ), ABraxane®, a cremophor-free, albumin-engineered nanoparticle formulation of paclitaxel (American Pharmaceutical Partners, Schaumberg, Ill.), and Taxotere® docetaxel (Rhone-Poulenc Rorer, Antony, France); chlorambucil; Gemzar® gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; platinum coordination complexes such as cisplatin, oxaliplatin, and carboplatin; vinblastine; platinum; etoposide (VP-16); ifosfamide; mitoxantrone; vincristine; Navelbine® vinorelbine; novantrone; teniposide; edatrexate; daunomycin; aminopterin; Xeloda; ibandronate; irinotecan (e.g., CPT-11); topoisomerase inhibitor RFS2000; difluoromethylornithine (DMFO); retinoids such as retinoic acid; capecitabine;and pharmaceutically acceptable salts, acids, or derivatives thereof. Two or more chemotherapeutic agents can be used in a cocktail administered in combination with the first therapeutic agent described herein. Suitable dosing regimens for combination chemotherapy are known in the art and are described, for example, in Saltz et al. (1999) Proc ASCO 18:233a and Douillard et al. (2000) Lancet 355:1041-7.

[0183] In some embodiments, the second therapeutic agent is a therapeutic agent that is a biologic, such as a cytokine (e.g., an interferon or an interleukin (e.g., IL-2)) used in cancer treatment. In some embodiments, the biologic is an anti-angiogenic agent, such as an anti-VEGF agent, e.g., bevacizumab (Avastin®). In some embodiments, the biologic is an immunoglobulin-based biologic, e.g., a monoclonal antibody (e.g., a humanized antibody, fully human antibody, Fc fusion protein, or functional fragment thereof) that agonizes a target to stimulate an anti-cancer response or antagonizes an antigen important to cancer. Such medications include Rituxan (rituximab), Zenapax (daclizumab), Simulect (basiliximab), Synagis (palivizumab), Remicade (infliximab), Herceptin (trastuzumab), Mylotarg (gemtuzumab ozogamicin), Campath (alemtuzumab), Zevalin (ibritumomab tiuxetan), Humira (adalimumab), Xolair (omalizumab), Bexxar (tositumomab-I-131), Raptiva (efalizumab), Erbitux (cetuximab), Avastin (bevacizumab), Tysabri (natalizumab), and Actemra (tosi Antibody-drug conjugates are also included.

[0184] The second agent may be a therapeutic agent that is a non-drug treatment, for example, the second therapeutic agent is radiation therapy, cryotherapy, thermotherapy, and / or surgical removal of tumor tissue.

[0185] The second agent may be a checkpoint inhibitor. In one embodiment, the checkpoint inhibitor is an inhibitory antibody (e.g., a monospecific antibody such as a monoclonal antibody). The antibody can be, for example, humanized or fully human. In some embodiments, the checkpoint inhibitor is a fusion protein, e.g., an Fc receptor fusion protein. In some embodiments, the checkpoint inhibitor is an agent, such as an antibody, that interacts with a checkpoint protein. In some embodiments, the checkpoint inhibitor is an agent, such as an antibody, that interacts with a ligand of the checkpoint protein. In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of CTLA-4 (e.g., an anti-CTLA4 antibody such as ipilimumab / Yervoy or tremelimumab). In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of PD-1 (e.g., nivolumab / Opdivo®; pembrolizumab / Keytruda®; pidilizumab / CT-011). In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of PDL1 (e.g., MPDL3280A / RG7446; MEDI4736; MSB0010718C; BMS 936559). In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or Fc fusion or small molecule inhibitor) of PDL2 (e.g., a PDL2 / Ig fusion protein such as AMP 224). In some embodiments, the checkpoint inhibitor is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of B7-H3 (e.g., MGA271), B7-H4, BTLA, HVEM, TIM3, GAL9, LAG3, VISTA, KIR, 2B4, CD160, CGEN-15049, CHK1, CHK2, A2aR, a B-7 family ligand, or a combination thereof.

[0186] In any of the combination embodiments described herein, the first and second therapeutic agents are administered simultaneously or sequentially, in either order. The first therapeutic agent can be administered immediately before, immediately after, up to 1 hour, up to 2 hours, up to 3 hours, up to 4 hours, up to 5 hours, up to 6 hours, up to 7 hours, up to 8 hours, up to 9 hours, up to 10 hours, up to 11 hours, up to 12 hours, up to 13 hours, 14 hours, up to 16 hours, up to 17 hours, up to 18 hours, up to 19 hours, up to 20 hours, up to 21 hours, up to 22 hours, up to 23 hours, up to 24 hours, or up to 1-7, 1-14, 1-21, or 1-30 days before or after the second therapeutic agent.

[0187] Pharmaceutical Compositions The compounds of the invention are preferably formulated into pharmaceutical compositions for administration to mammals, preferably humans, in a biologically compatible form suitable for administration in vivo. Thus, in one aspect, the invention provides a pharmaceutical composition comprising a compound of the invention in admixture with a suitable diluent, carrier, or excipient.

[0188] The compounds of the present invention may be used in the form of free base, salt, solvate, and prodrug. All forms are within the scope of the present invention. According to the method of the present invention, as will be understood by those skilled in the art, the described compounds, or their salts, solvates, or prodrugs, can be administered to patients in various forms depending on the selected route of administration. The compounds of the present invention may be administered, for example, orally, parenterally, bucally, sublingually, nasally, rectally, via patch, pump, or transdermal administration, and the pharmaceutical composition will be formulated accordingly. Parenteral administration includes intravenous, intraperitoneal, subcutaneous, intramuscular, transepithelial, nasal, intrapulmonary, intrathecal, rectal, and topical modes of administration. Parenteral administration may also be by continuous infusion over a selected period of time.

[0189] The compounds of the present invention can be administered orally, for example, with an inert diluent or an assimilable edible carrier, or enclosed in hard or soft shell gelatin capsules, compressed into tablets, or incorporated directly with dietary food. For oral therapeutic administration, the compounds of the present invention can be incorporated with excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, and wafers. The compounds of the present invention can also be administered parenterally. Solutions of the compounds of the present invention can be prepared in water suitably mixed with a surfactant such as hydroxypropyl cellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, DMSO, and mixtures thereof, with or without alcohol, and in oils. These preparations may contain preservatives to prevent the growth of microorganisms under ordinary storage and use conditions. Conventional procedures and ingredients for the selection and preparation of suitable formulations are described, for example, in Remington's Pharmaceutical Sciences (2003, 20th ed.) and The United States Pharmacopeia: The National Formulary (USP 24 NF19), published in 1999. Pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and sufficiently fluid for easy administration via syringe. Compositions for nasal administration can be conveniently formulated as aerosols, drops, gels, and powders. Aerosol formulations typically comprise a solution or fine suspension of the active substance in a physiologically acceptable aqueous or non-aqueous solvent and are usually provided in single or multi-dose sterile form in a sealed container, which may be in the form of a cartridge or refill for use with a nebulizer device. Alternatively, the sealed container may be a single-dispensing device, such as a single-dose nasal inhaler or aerosol dispenser fitted with a metering valve, intended to be discarded after use.Where the dosage form comprises an aerosol dispenser, it contains a propellant, which may be a compressed gas such as compressed air or an organic propellant such as a fluorochlorohydrocarbon. The aerosol dosage form may also take the form of a pump-type atomizer. Compositions suitable for buccal or sublingual administration include tablets, lozenges, and pastilles in which the active ingredient is formulated with a carrier such as sugar, acacia, tragacanth, gelatin, and glycerin. Compositions for rectal administration are conveniently in the form of suppositories containing a conventional suppository base such as cocoa butter. The compounds described herein may be administered intratumorally, for example, by intratumoral injection. Intratumoral injection is a direct injection into the tumor vasculature and is particularly intended for individual, solid, accessible tumors. Local, regional, or systemic administration may also be appropriate. The compounds described herein may be advantageously contacted with the tumor by injection or multiple injections, for example, spaced approximately 1 cm apart. In the case of surgical intervention, the present invention may be used preoperatively, such as to subject an inoperable tumor to resection. Continuous administration can also be applied where appropriate, for example, by implanting a catheter into the tumor or tumor vasculature.

[0190] The compounds of the invention, as described herein, can be administered to animals, e.g., humans, alone or in combination with pharmaceutically acceptable carriers, the ratios being determined by the solubility and chemical properties of the compounds, the chosen route of administration, and standard pharmaceutical practice.

[0191] Dosage The dosage of the compounds of the present invention and / or compositions containing the compounds of the present invention can vary depending on many factors, including the pharmacodynamic properties of the compound; the mode of administration; the age, health, and weight of the recipient; the nature and severity of the symptoms; the frequency of treatment and the type of concomitant treatment, if any; and the clearance rate of the compound in the treated animal. Those skilled in the art can determine the appropriate dosage based on the above factors. The compounds of the present invention may be initially administered at a suitable dosage, which may be adjusted as necessary depending on the clinical response. In general, satisfactory results can be obtained when the compounds of the present invention are administered to humans at a daily dose of, for example, 0.05 mg to 3000 mg (measured as solid form). Dose ranges include, for example, 10 to 1000 mg (e.g., 50 to 800 mg). In some embodiments, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg of the compound is administered.

[0192] Alternatively, the patient's body weight can be used to calculate the dosage. For example, the dose of a compound or pharmaceutical composition thereof administered to a patient may be in the range of 0.1 to 100 mg / kg (e.g., 0.25 to 25 mg / kg). In exemplary, non-limiting embodiments, the dose may be in the range of 0.5 to 5.0 mg / kg (e.g., 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg / kg) or 5.0 to 20 mg / kg (e.g., 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg / kg). [Example]

[0193] The following abbreviations are used throughout the examples below: [Table 3-1] [Table 3-2]

[0194] Example 1. Preparation of intermediates Preparation of 2-(6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-1) [ka] Step 1: Preparation of tert-butyl 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate [ka] To a stirred solution of 4-bromo-6-chloropyridazin-3-amine (345 mg, 1.66 mmol) and tert-butyl 3-ethynylazetidine-1-carboxylate (300 mg, 1.66 mmol) in DMF (10.0 mL) was added Pd(PPh)Cl (383 mg, 0.331 mmol), CuI (63.1 mg, 0.331 mmol), and EtN (1.68 g, 16.6 mmol) at room temperature. The resulting mixture was stirred at 120 °C for 16 hours under a nitrogen atmosphere. The residue was purified by reverse-phase C18 flash chromatography (water:ACN:FA) to give tert-butyl 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (175 mg, 34.2%) as a black solid. LCMS(ESI)m / z:[M+H] + =309.

[0195] Step 2: Preparation of tert-butyl 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (175 mg, 0.567 mmol) and 2-hydroxyphenylboronic acid (156 mg, 1.13 mmol) in dioxane (5.00 mL) and HO (1.00 mL), XPhos Pd G3 (48.0 mg, 0.057 mmol) and CsCO3 (554 mg, 1.70 mmol) were added at room temperature under a nitrogen atmosphere. The resulting mixture was stirred at 100 °C for 4 hours under a dry nitrogen atmosphere. The reaction was quenched by adding water at room temperature. The resulting mixture was extracted three times with EtOAc. The combined organic layers were washed with brine and dried over anhydrous NaSO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase C18 flash chromatography (water:ACN:FA) to give tert-butyl 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (138 mg, 66.5%) as a pale yellow solid. LCMS (ESI) m / z: [M+H] + =367.

[0196] Step 3: Preparation of 2-[6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-1) [ka] To a stirred solution of tert-butyl 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (30.0 mg, 0.082 mmol) in DCM (2.00 mL) was added TFA (1.00 mL) dropwise at room temperature. The resulting mixture was stirred at room temperature for 1 hour. The resulting mixture was concentrated under reduced pressure. The crude product was purified by preparative HPLC (water:ACN:FA) to give I-1 (11.0 mg, 50.5%) as an off-white solid. 1H NMR(400MHz,DMSO-d6)δ 13.94(s,1H),9.11(s,1H),8.66(s,1H),8.06(dd,J=8.0,1.6Hz,1H),7.31(ddd,J=8.5,7. 2,1.6Hz,1H),6.97(t,J=7.8Hz,2H),6.76(s,1H),4.45-4.23(m,5H).LCMS(ESI)m / z:[M+H] + =267.20.

[0197] The following intermediates in Table 3 were prepared from 4-bromo-6-chloropyridazin-3-amine and the appropriate alkyne in a manner similar to that described in the preparation of intermediate I-1. [Table 4-1] [Table 4-2]

[0198] Preparation of 2-[5-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-2) [ka] Step 1: Preparation of tert-butyl 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylate [ka] To a stirred solution of 4-bromo-6-chloropyridazin-3-amine (300 mg, 1.44 mmol) and tert-butyl 3-(2-oxoethyl)azetidine-1-carboxylate (287 mg, 1.44 mmol) in DMF (5.00 mL) was added Pd(OAc) (32.3 mg, 0.144 mmol), (t-Bu)P·HBF (41.8 mg, 0.144 mmol), and 1,4-diazabicyclo[2.2.2]octane (484 mg, 4.32 mmol) at room temperature. After stirring at 85 °C for 16 h under a nitrogen atmosphere, the mixture was allowed to cool to room temperature. The reaction mixture was filtered through a short pad of Celite and concentrated in vacuo. The residue was purified by reverse-phase C18 flash chromatography (water:ACN:FA) to give tert-butyl 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylate (115 mg, 25.9%) as a brown solid.

[0199] Step 2: Preparation of 2-[5-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-2) [ka] To a stirred solution of 3-[3-chloro-7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylic acid (115 mg, 0.372 mmol) and tert-butyl 2-hydroxyphenylboronate (154 mg, 1.12 mmol) in 1,4-dioxane (8.00 mL) and HO (2.00 mL) was added XPhos Pd G3 (62.6 mg, 0.074 mmol) and Cs2CO3 (364 mg, 1.12 mmol) at room temperature. After stirring at 80 °C under a nitrogen atmosphere for 1 h, the mixture was allowed to cool to room temperature. The reaction mixture was filtered through a short pad of Celite and concentrated in vacuo. The residue was purified by preparative HPLC (water:ACN:FA) to afford I-2 (5.7 mg, 4.19%) as a yellow solid. 1H NMR(400MHz,DMSO-d6)δ 14.03(s,1H),8.87(s,1H),8.45(s,1H),8.27-8.05(m,2H),7.31(t,J=7.8Hz,1H ),7.07-6.92(m,2H),4.39-4.30(m,1H),4.26-4.10(m,4H).LCMS(ESI)m / z:[M+H] + =267.05.

[0200] Preparation of 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (I-10) [ka] Step 1: Preparation of methyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate [ka] To a solution of 10-methoxy-10-oxo-decanoic acid (136 mg, 0.627 mmol) and (2S,4R)-1-[(2S)-2-amino-3,3-dimethyl-butanoyl]-4-hydroxy-N-[[4-(4-methylthiazol-5-yl)phenyl]methyl]pyrrolidine-2-carboxamide (300 mg, 0.697 mmol) in DCM (3 mL) was added HATU (265 mg, 0.697 mmol) and DIEA (485 μL, 2.79 mmol). After the addition, the mixture was stirred at 25 °C for 2 h. The reaction mixture was quenched with water and then extracted three times with DCM. The combined organic layers were washed twice with brine, dried over Na SO , filtered, and concentrated under reduced pressure to give a residue. The residue was purified by reverse phase chromatography to give methyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate (175 mg, 39.9% yield) as a yellow oil. LCMS (ESI) m / z: [M+H] + = 629.5.

[0201] Step 2: Preparation of 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (I-10) [ka] To a solution of methyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate (170 mg, 0.270 mmol) in MeOH (1.5 mL) and HO (0.5 mL) was added NaOH (21.6 mg, 0.541 mmol) at 25 °C. The mixture was stirred at this temperature for 12 h. The reaction mixture was adjusted to neutral pH with hydrochloric acid (2 M). The residue was purified by reverse phase chromatography to give 10-(((S)-1-((2S,4R)-4-hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (115 mg, 69.5% yield) as a white solid. 1 H NMR(400MHz,DMSO-d6)δ 12.25-11.73(m,1H),8.99(s,1H),8.57 -8.55(m,1H),7.84(d,J=9.2Hz,1H),7.45-7.36(m,4H),5.24-5.05(m,1H),4.56-4.21(m,5H),3.71-3.61(m, 2H),2.45(s,3H),2.28-1.89(m,7H),1.54-1.41(m,4H),1.24(s,8H),0.97-0.91(m,9H).LCMS(ESI)m / z:[M+H] + = 615.5.

[0202] The following intermediates in Table 4 were prepared from (2S,4R)-1-[(2S)-2-amino-3,3-dimethyl-butanoyl]-4-hydroxy-N-[[4-(4-methylthiazol-5-yl)phenyl]methyl]pyrrolidine-2-carboxamide and the appropriate carboxylic acid in a manner similar to that described in the preparation of intermediate I-10. [Table 5]

[0203] Preparation of 7-[[(2S)-1-[(2S,4R)-4-hydroxy-2-([[4-(4-methyl-1,3-thiazol-5-yl)phenyl]methyl]carbamoyl)pyrrolidin-1-yl]-3,3-dimethyl-1-oxobutan-2-yl]carbamoyl]heptanoic acid (I-50) [ka] To a stirred solution of octanedioic acid (2.02 g, 11.6 mmol) in DCM (25.0 mL) and THF (25.0 mL) was added (2S,4R)-1-((S)-2-amino-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide (1.00 g, 2.32 mmol), TEA (823 mg, 8.13 mmol), HOAt (348 mg, 2.56 mmol), and EDCI (490 mg, 2.56 mmol) at 0 °C. The resulting solution was stirred for 2 h at 0 °C. The resulting mixture was concentrated under reduced pressure, and the residue was purified by reverse-phase flash chromatography to afford I-50 (900 mg, 66.0%) as a white solid. LCMS (ESI) m / z: [M+H] + =587.

[0204] Preparation of 4-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxo-2,3-dihydro-1H-isoindol-4-yl]oxy]butanoic acid (I-29) [ka] Step 1: Preparation of tert-butyl 4-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-4-yl]oxy]butanoate [ka] To a solution of 2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindole-1,3-dione (2.00 g, 7.29 mmol) and tert-butyl 4-bromobutanoate (1.95 g, 8.752 mmol) in DMF (10.0 mL) was added KI (0.12 g, 0.729 mmol) and KHCO (1.10 g, 10.9 mmol). The resulting solution was stirred at 60 °C for 5 h. The mixture was diluted with EtOAc and washed three times with water. The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by reverse-phase C18 flash chromatography (water:ACN) to give tert-butyl 4-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-4-yl]oxy]butanoate (1.5 g, 49.4%) as an off-white solid. LCMS (ESI) m / z [M+H] + =417.

[0205] Step 2: Preparation of 4-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxo-2,3-dihydro-1H-isoindol-4-yl]oxy]butanoic acid (I-29) [ka] To a stirred solution of tert-butyl 4-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-4-yl]oxy]butanoate (450 mg, 1.08 mmol) in DCM (5 mL) was added TFA (1 mL). The resulting solution was stirred for 2 hours at 25° C. The resulting mixture was concentrated. This afforded I-29 (360 mg, 92.5%) as a white solid. 1 H NMR(400MHz, methanol-d4)δ 7.79(t,J=8.4,7.4Hz,1H),7.47(d,J=7.8Hz,2H),5.12(dd,J=12.6,5.5Hz,1H),4.30(t,J=6 .2Hz,2H),2.95-2.66(m,3H),2.60(t,J=7.3Hz,2H),2.25-2.18(m,3H).LCMS(ESI)m / z:[M+H] + =361.10.

[0206] The following intermediates in Table 5 were prepared from the appropriate alkyl bromides in a manner similar to that described in the preparation of intermediate I-29. [Table 6]

[0207] Preparation of 9-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-5-yl]amino]nonanoic acid (I-3) [ka] Step 1: Preparation of methyl 9-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-5-yl]amino]nonanoate [ka] To a solution of 2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindole-1,3-dione (1.00 g, 3.62 mmol) and methyl 9-aminononanoate (814 mg, 4.34 mmol) in NMP (10.0 mL) was added DIEA (2.34 g, 18.1 mmol). The reaction mixture was heated to 90 °C under N for 5 h. The resulting mixture was diluted with water and extracted three times with EtOAc. The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (PE / EtOAc) to give methyl 9-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-5-yl]amino]nonanoate (297 mg, 18.5%) as a yellow-green solid. LCMS (ESI) m / z [M+H] + =444.

[0208] Step 2: Preparation of 9-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-5-yl]amino]nonanoic acid (I-3) [ka] To a stirred solution of methyl 9-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-5-yl]amino]nonanoate (330 mg, 0.744 mmol) in DCM (10 mL) was added TFA (10 mL) dropwise at room temperature. After stirring for 2 h, the resulting mixture was concentrated under reduced pressure, and the residue was purified by flash chromatography to give I-3 (290 mg, 90.8%) as a yellow solid. 1 H NMR(400MHz, methanol-d4)δ 7.58(d,1H),6.99(d,1H),6.85(dd,1H),5.06(dd,1H),3.22(t,2H),2.88-2. 71(m,2H),2.30(t,2H),1.69-1.59(m,4H),1.38(s,8H);LCMS(ESI)m / z:[M+H] + =430.19.

[0209] Preparation of (2S,4R)-1-((R)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (I-4) [ka] Step 1: Preparation of 2-(3-bromoisoxazol-5-yl)acetic acid [ka] To a stirred solution of 2-(3-bromo-1,2-oxazol-5-yl)ethan-1-ol (30 g, 156 mmol) in acetone (389 mL) was added Jones reagent (2 M in acetone, 156 mL, 312 mmol) dropwise at 0 °C. The resulting solution was stirred at 25 °C overnight. The mixture was diluted with water and extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure to give 2-(3-bromoisoxazol-5-yl)acetic acid (28 g, 86.5%) as a brown solid. LCMS (ESI) m / z: [M+H] + =206.08 and 208.08.

[0210] Step 2: Preparation of methyl 2-(3-bromoisoxazol-5-yl)acetate [ka] A solution of 2-(3-bromoisoxazol-5-yl)acetic acid (28 g, 135 mmol) and concentrated HSO (3 mL, 72 mmol) in methanol (250 mL) was stirred at 70 °C for 2 h. The resulting solution was concentrated under reduced pressure. The residue was diluted with water and extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous MgSO, and concentrated under reduced pressure. The residue was purified by silica gel flash chromatography (EtOAc / petroleum ether) to give methyl 2-(3-bromoisoxazol-5-yl)acetate (23.4 g, 79%) as a white solid. LCMS (ESI) m / z: [M+H] + =219.90 and 221.86.

[0211] Step 3: Preparation of methyl 2-(3-bromoisoxazol-5-yl)-3-methylbutanoate [ka] Methyl 2-(3-bromoisoxazol-5-yl)acetate (23.4 g, 106 mmol) and KO in THF (210 mL) t To a stirred solution of Bu (17.8 g, 159 mmol), 2-iodopropane (13.8 mL, 137 mmol) was added dropwise at 0 °C. The reaction mixture was stirred at room temperature for 16 h and then quenched with water / ice. The resulting solution was extracted several times with EtOAc. The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by silica gel flash chromatography (EtOAc / petroleum ether) to give methyl 2-(3-bromoisoxazol-5-yl)-3-methylbutanoate (16.7 g, 60%) as a clear oil.

[0212] Step 4: Preparation of 2-(3-methoxyisoxazol-5-yl)-3-methylbutanoic acid [ka] To a solution of methyl 2-(3-bromo-1,2-oxazol-5-yl)-3-methylbutanoate (16.7 g, 63.7 mmol) in methanol (130 mL) was added potassium hydroxide (35.7 g, 637 mmol). The mixture was stirred at 100 °C for 4 h. The mixture was concentrated in vacuo and then diluted with water. The resulting solution was washed with EtOAc, and the pH of the aqueous layer was adjusted to pH 5 with 1 N HCl. The mixture was extracted several times with EtOAc. The combined organic layers were washed with brine and dried over anhydrous MgSO4. The residue was purified by silica gel flash chromatography (EtOAc / petroleum ether) to give 2-(3-methoxyisoxazol-5-yl)-3-methylbutanoic acid (8.8 g, 70%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =200.15.

[0213] Step 5: Preparation of 2-(3-hydroxyisoxazol-5-yl)-3-methylbutanoic acid [ka] A solution of 2-(3-methoxyisoxazol-5-yl)-3-methylbutanoic acid (8.8 g, 44.1 mmol) in HOAc (80 mL) and HBr (80 mL) was stirred for 16 h at 60° C. The resulting mixture was concentrated under reduced pressure to give crude 2-(3-hydroxyisoxazol-5-yl)-3-methylbutanoic acid (8.16 g, quantitative).

[0214] Step 6: Preparation of methyl 2-(3-hydroxyisoxazol-5-yl)-3-methylbutanoate [ka] To a solution of 2-(3-hydroxy-1,2-oxazol-5-yl)-3-methylbutanoic acid (8.16 g, 44.0 mmol) in methanol (30 mL) was slowly added SOCI2 (14.2 mL, 197 mmol). The mixture was stirred at room temperature for 3 hours. The solvent was removed under reduced pressure. The residue was diluted with water and extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by silica gel flash chromatography (MeOH / DCM) to give methyl 2-(3-hydroxyisoxazol-5-yl)-3-methylbutanoate (7.79 g, 89%) as a clear oil. LCMS (ESI) m / z: [M+H] + =200.15.

[0215] Step 7: Preparation of methyl 2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoate [ka] To a solution of methyl 2-(3-hydroxy-1,2-oxazol-5-yl)-3-methylbutanoate (7.79 g, 39.1 mmol) in DMF (90 mL) was added 2-bromo-1,1-diethoxyethane (8.77 mL, 58.6 mmol) and potassium carbonate (10.8 g, 78.2 mmol). The reaction was stirred at 70 °C overnight. The reaction mixture was cooled and then added to the mixture. The resulting mixture was extracted several times with EtOAc. The combined organic layers were washed with brine and dried over anhydrous MgSO. The solvent was removed under reduced pressure, and the resulting residue was purified by silica gel flash chromatography (EtOAc:heptane) to afford methyl 2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoate (7.8 g, 63%) as a colorless oil. LCMS (ESI) m / z: [M-CHO] + =270.30.

[0216] Step 8: Preparation of 2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoic acid [ka] To a solution of methyl 2-[3-(2,2-diethoxyethoxy)-1,2-oxazol-5-yl]-3-methylbutanoate (7.8 g, 24.7 mmol) in methanol (50 mL) and water (25 mL) was added lithium hydroxide monohydrate (4.14 g, 98.8 mmol). The reaction was stirred at 40 °C for 2 h. The pH was adjusted to 4-5 with 1 N HCl. The mixture was extracted several times with ethyl acetate, and the combined organic layers were dried over MgSO. The solvent was removed under reduced pressure, and the residue was purified by silica gel flash chromatography (DCM:MeOH) to give 2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoic acid (6.1 g, 89%) as a colorless oil. LCMS (ESI) m / z: [MH] - =300.21.

[0217] Step 9: Preparation of tert-butyl (2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidine-1-carboxylate [ka] To a solution of (S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethan-1-amine hydrochloride (5.0 g, 19.6 mmol) and (2S,4R)-1-[(tert-butoxy)carbonyl]-4-hydroxypyrrolidine-2-carboxylic acid (4.47 g, 20.5 mmol) in DCM (70 mL) at 0 °C, HATU (8.98 g, 23.5 mmol) was added, followed by dropwise addition of DIEA (16.4 mL, 98.0 mmol). After stirring for 16 h at room temperature, the reaction mixture was poured into ice water. The resulting mixture was extracted several times with DCM. The combined organic layers were washed with water, brine, dried over anhydrous NaSO, and concentrated in vacuo. The resulting residue was purified by silica gel flash chromatography (MeOH:DCM) to give tert-butyl (2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidine-1-carboxylate (8.33 g, 98%). LCMS (ESI) m / z: [M+H] + =432.38.

[0218] Step 10: Preparation of (2S,4R)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide hydrochloride [ka] To a stirred solution of tert-butyl (2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidine-1-carboxylate (8.33 g, 19.3 mmol) at 0° C. was added HCl in 1,4-dioxane (4 N, 50 mL, 200 mmol) to give a viscous yellow gum. 15 mL of MeOH was added to the mixture, and the mixture was stirred at room temperature for 2 hours. The solvent was removed under reduced pressure, and the residue was washed with diethyl ether to give (2S,4R)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide hydrochloride, which was used in the next step without further purification.

[0219] Step 11: Preparation of (2S,4R)-1-((R)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (I-4) [ka] To a solution of 2-[3-(2,2-diethoxyethoxy)isoxazol-5-yl]-3-methyl-butanoic acid (5.75 g, 19.0 mmol) in DMF (30 mL) was added HATU (8.6 g, 22.7 mmol). After stirring at 20 °C for 0.5 h, a solution of (2S,4R)-4-hydroxy-N-[(1S)-l-[4-(4-methylthiazol-5-yl)phenyl]ethyl]pyrrolidine 2-carboxamide hydrochloride (6.97 g, 19.0 mmol) and triethylamine (7.92 mL, 56.9 mmol) in DMF (20 mL) was added to the mixture, and the resulting mixture was stirred at 20 °C. The reaction mixture was quenched by adding water and extracted several times with EtOAc. The combined organic layers were washed with brine, dried over anhydrous MgSO4, filtered, and concentrated under reduced pressure. The residue was purified by silica gel flash chromatography (DCM:MeOH) to give (2S,4R)-1-[2-[3-(2,2-diethoxyethoxy)isoxazol-5-yl]-3-methyl-butanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methylthiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (10 g, 16.2 mmol) as a white solid. The mixture of diastereomers was separated by chiral SFC chromatography to give (2S,4R)-1-((S)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide and (2S,4R)-1-((R)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide. (2S,4R)-1-((S)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide, Peak 1: (2.2 g, 19%). LCMS (ESI) m / z [M+H]+ =615.4. (2S,4R)-1-((R)-2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (I-4), Peak 2: (2.5 g, 21%). LCMS (ESI) m / z [M+H] + =615.4.

[0220] Step 12: Preparation of (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (I-5) [ka] To a stirred solution of H2SO4 (1N, 6.00 mL) and THF (6.00 mL), (2S,4R)-1-[(2R)-2-[3-(2-ethoxy-2-methoxyethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (300 mg, 0.499 mmol) was added dropwise at room temperature. The resulting mixture was stirred for 8 hours at 50 °C. The resulting mixture was diluted with water and neutralized to approximately pH 7 with saturated aqueous NaHCO3. The resulting mixture was extracted three times with EtOAc. The combined organic layers were washed twice with brine and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure to give (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (I-5, 256 mg, 97.3%) as a white solid. LCMS (ESI) m / z: [M+H] + =541.

[0221] Preparation of methyl 2-[4-chloro-3-(2,2-diethoxyethoxy)-1,2-oxazol-5-yl]-3-methylbutanoate (I-59) [ka] A solution of methyl 2-(3-(2,2-diethoxyethoxy)isoxazol-5-yl)-3-methylbutanoate (300 mg, 0.951 mmol, 1.00 equiv) and NCS (152.43 mg, 1.141 mmol, 1.2 equiv) in DMF (3.00 mL) was stirred at 70°C for 12 hours. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN (0.05% FA) in water, gradient 0% to 100% in 25 minutes; detector, UV 254 nm. This afforded I-59 (180 mg, 54.09%) as a white solid. LCMS (ESI) m / z: [M+H] + =350.

[0222] The following intermediates in Table 6 were prepared in a manner similar to that described in the preparation of intermediate I-5, starting from methyl 2-(3-hydroxy-1,2-oxazol-5-yl)-3-methylbutanoate and the appropriate alkyl bromide. [Table 7-1] [Table 7-2]

[0223] Preparation of 2-((5-((R)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(2-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3-methyl-1-oxobutan-2-yl)isoxazol-3-yl)oxy)acetic acid (I-67) [ka] To a stirred solution of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(2-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(2-oxoethoxy)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (30.00 mg, 0.055 mmol, 1.00 equiv) and 2-methyl-2-butene (0.78 mg, 0.011 mmol, 0.20 equiv) in ter-butanol (2 mL) was added dropwise a solution of NaClO (50.19 mg, 0.550 mmol, 10.00 equiv) and NaHPO (78.77 mg, 0.550 mmol, 10.00 equiv) in water (2.00 mL) at 0 °C. The mixture was stirred at 0 °C for 0.5 h, then allowed to warm to room temperature and stirred for an additional 1.5 h. The reaction was quenched with a mixture of saturated NaSO solution and brine and extracted with CHCl (20 mL × 3). The combined organic extracts were dried over NaSO, concentrated in vacuo, and purified by silica gel chromatography (PE / EtOAc = 1 / 3 to 1 / 1). This afforded intermediate I-67 (15.80 mg, 49.93%) as a colorless oil. LCMS (ESI) m / z: [M+H] + =557.

[0224] Preparation of (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methyl)pyrrolidine-2-carboxamide (I-68) [ka] Step 1: Preparation of methyl 3-(hydroxymethyl)bicyclo[1.1.1]pentane-1-carboxylate [ka] A solution of 3-(methoxycarbonyl)bicyclo[1.1.1]pentane-1-carboxylic acid (5.00 g, 29.383 mmol, 1.00 equiv) in THF (50.00 mL) was treated with borane (0.61 g, 0.044 mmol, 1.50 equiv). The resulting mixture was stirred overnight at room temperature. The reaction was quenched with water at 0 degrees Celsius and extracted with EtOAc (3 x 500 mL). The combined organic layers were washed with brine (2 x 100 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The crude product was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =157.

[0225] Step 2: Preparation of methyl 3-formylbicyclo[1.1.1]pentane-1-carboxylate [ka] To a stirred mixture of oxalyl chloride (1.22 g, 9.60 mmol, 1.50 equiv) in DCM (20.00 mL) was added DMSO (1.5 g, 19.21 mmol, 3.00 equiv) dropwise under a dry nitrogen atmosphere at −78 °C. The resulting mixture was stirred for 15 minutes at −78 °C under a dry nitrogen atmosphere. To the above mixture was added methyl 3-(hydroxymethyl)bicyclo[1.1.1]pentane-1-carboxylate (1.00 g, 6.40 mmol, 1.00 equiv) at −78 °C. The resulting mixture was stirred for an additional 30 minutes at −78 °C. To the above mixture was added EtN (3.89 g, 38.42 mmol, 6.00 equiv) at −78 °C. The resulting mixture was stirred for an additional 30 minutes at −78 °C. The resulting mixture was extracted with EtOAc (3 x 200 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give methyl 3-formylbicyclo[1.1.1]pentane-1-carboxylate (400 mg, 32.42%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =155.

[0226] Step 3: Preparation of methyl (E)-3-(prop-1-en-1-yl)bicyclo[1.1.1]pentane-1-carboxylate [ka] To a stirred solution of ethyltriphenylphosphanium bromide (13.00 g, 35.028 mmol, 3 equiv) in THF (150 mL) was added t-BuOK (3.28 g, 29.190 mmol, 2.5 equiv) at 0°C. The resulting mixture was stirred at 0°C for 1 hour. To the above mixture was added methyl 3-formylbicyclo[1.1.1]pentane-1-carboxylate (1.8 g, 11.676 mmol, 1.00 equiv) in THF (10 mL) dropwise over 15 minutes at 0°C. The resulting mixture was stirred at room temperature for an additional 3 hours under a N2 atmosphere. The mixture was acidified to pH 7 with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (3 x 200 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (10:1) to give methyl (E)-3-(prop-1-en-1-yl)bicyclo[1.1.1]pentane-1-carboxylate (1.16 g, 60%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =167.

[0227] Step 4: Preparation of methyl 3-(2-amino-4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate [ka] To a stirred solution of IBX (3.033 g, 10.80 mmol, 2 equiv.) and I2 (1.59 g, 6.00 mmol, 1.1 equiv.) in DMSO (100 mL) was added (E)-methyl 3-(prop-1-en-1-yl)bicyclo[1.1.1]pentane-1-carboxylate (900 mg, 5.40 mmol, 1.00 equiv.) in one portion at room temperature. The reaction mixture was stirred at room temperature until the starting alkene was completely consumed (monitored by LCMS). It was then diluted with DCM (100 mL) and washed with saturated aqueous NaHCO3-Na2S2O3. The aqueous layer was extracted with DCM (2 × 100 mL), and the combined organic layers were dried over Na2SO4 and filtered. Thiourea (1.24 g, 16.20 mmol, 3 equiv.) and dimethylformamide (100 mL) were added to the above mixture. The reaction mixture was stirred at room temperature for 12 hours. The reaction mixture was washed with water (3 x 300 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (2:1) to give methyl 3-(2-amino-4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate (670.6 mg, 52.0%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =239.

[0228] Step 5: Preparation of methyl 3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate [ka] To a stirred solution of methyl 3-(2-amino-4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate (654 mg, 2.75 mmol, 1.00 equiv) in THF (50 mL) was added t-BuNO (1.41 g, 13.74 mmol, 5 equiv) at room temperature. The resulting mixture was stirred at 60°C for 1 hour. The mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give methyl 3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate (145.8 mg, 23.5%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =224.

[0229] Step 6: Preparation of 3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxamide [ka] To a stirred solution of methyl 3-(4-methyl-1,3-thiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxylate (144 mg, 0.645 mmol, 1.00 equiv) was added ammonia in methanol (15 mL) at room temperature. The resulting mixture was stirred at 50°C for 16 hours. The resulting mixture was concentrated under reduced pressure. This afforded 3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxamide (72 mg, 53.60%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =209.

[0230] Step 7: Preparation of (3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methanamine [ka] To a stirred solution of 3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentane-1-carboxamide (72 mg, 0.346 mmol, 1.00 equiv) in THF (2 mL) was added LiAlH (0.3 mL, 2.5 M) at 0°C under a dry nitrogen atmosphere. The resulting mixture was stirred at room temperature for 16 hours under a dry nitrogen atmosphere. The mixture was acidified to pH 7 with saturated NH Cl (aq). The resulting mixture was extracted with EtOAc (3 × 20 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous Na SO . After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give (3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methanamine (60.3 g, 90.00%) as a yellow oil. LCMS(ESI)m / z:[M+H] + =195.

[0231] Preparation of (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methyl)pyrrolidine-2-carboxamide (I-69) [ka] (2S,4R)-4-Hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methyl)pyrrolidine-2-carboxamide was prepared in a similar manner as described in the preparation of intermediate I-5, starting from (3-(4-methylthiazol-5-yl)bicyclo[1.1.1]pentan-1-yl)methanamine. LCMS (ESI) m / z: [M+H] + =517.

[0232] (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (I-70) and Preparation of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (I-71) [ka] Step 1: Preparation of methyl 3-methyl-2-[3-[(1,1,2,2,3,3,4,4,4-nonafluorobutanesulfonyl)oxy]-1,2-oxazol-5-yl]butanoate [ka] To a stirred solution of methyl 2-(3-hydroxy-1,2-oxazol-5-yl)-3-methylbutanoate (100.00 mg, 0.502 mmol, 1.00 equiv) in MeCN (0.50 mL) was added perfluorobutanesulfonyl fluoride (303.29 mg, 1.004 mmol, 2.00 equiv) and K2CO3 (208.13 mg, 1.506 mmol, 3.00 equiv) at room temperature. The resulting mixture was stirred for 3 h and then carefully quenched with water at 0 °C. The resulting mixture was extracted with EA (2 × 50 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (2 / 1) to give methyl 3-methyl-2-[3-[(1,1,2,2,3,3,4,4,4-nonafluorobutanesulfonyl)oxy]-1,2-oxazol-5-yl]butanoate (217 mg, crude) as a white solid. LCMS (ESI) m / z: [M+H] + =482.

[0233] Step 2: Preparation of tert-butyl 4-[5-(1-methoxy-3-methyl-1-oxobutan-2-yl)-1,2-oxazol-3-yl]piperazine-1-carboxylate [ka] To a stirred solution of methyl 3-methyl-2-[3-[(1,1,2,2,3,3,4,4,4-nonafluorobutanesulfonyl)oxy]-1,2-oxazol-5-yl]butanoate (217.00 mg, 0.451 mmol, 1.00 equiv) in DMF (3.00 mL) was added tert-butyl piperazine-1-carboxylate (83.98 mg, 0.451 mmol, 1.00 equiv) at room temperature. The resulting mixture was stirred at 130° C. for 1 hour. The mixture was allowed to cool to room temperature. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, gradient 0 to 100% in 30 min. This gave tert-butyl 4-[5-(1-methoxy-3-methyl-1-oxobutan-2-yl)-1,2-oxazol-3-yl]piperazine-1-carboxylate (54 mg, 32.59%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =368.

[0234] Step 3: Preparation of 2-[3-[4-(tert-butoxycarbonyl)piperazin-1-yl]-1,2-oxazol-5-yl]-3-methylbutanoic acid [ka] To a stirred solution of tert-butyl 4-[5-(1-methoxy-3-methyl-1-oxobutan-2-yl)-1,2-oxazol-3-yl]piperazine-1-carboxylate (54.00 mg, 0.147 mmol, 1.00 equiv) in MeOH (0.80 mL) was added THF (0.80 mL) and HO (0.80 mL) at room temperature, followed by LiOH .HO (18.50 mg, 0.441 mmol, 3.00 equiv) was added. The resulting mixture was stirred for an additional 1 h at room temperature. The mixture was acidified to pH 6 with HCl (1 M, aq) and then extracted with EA (2 × 50 mL). The combined organic layers were washed with saturated brine (50 mL), dried over anhydrous NaSO, and filtered. The filtrate was concentrated under reduced pressure. This gave 2-[3-[4-(tert-butoxycarbonyl)piperazin-1-yl]-1,2-oxazol-5-yl]-3-methylbutanoic acid (52 mg, crude) as a yellow solid. LCMS (ESI) m / z: [M+H] + =354.

[0235] Step 4: Preparation of tert-butyl 4-(5-[1-[(2S,4R)-4-hydroxy-2-[[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-1,2-oxazol-3-yl)piperazine-1-carboxylate [ka] To a stirred solution of 2-[3-[4-(tert-butoxycarbonyl)piperazin-1-yl]-1,2-oxazol-5-yl]-3-methylbutanoic acid (52.00 mg, 0.119 mmol, 1.00 equiv) in DMF (2.00 mL) was added HATU (135.56 mg, 0.357 mmol, 3.00 equiv) and DIEA (76.80 mg, 0.595 mmol, 5.00 equiv) at room temperature. To the above mixture was added (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (70.90 mg, 0.214 mmol, 1.80 equiv) at room temperature. The resulting mixture was stirred for an additional 1 h. The mixture was directly purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min. This afforded tert-butyl 4-(5-[1-[(2S,4R)-4-hydroxy-2-[[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-1,2-oxazol-3-yl)piperazine-1-carboxylate (73 mg, 92.12%) as a white solid. LCMS (ESI) m / z: [M+H] + =667.

[0236] Step 5: Preparation of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide; (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide [ka] tert-Butyl 4-(5-[1-[(2S,4R)-4-hydroxy-2-[[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-1,2-oxazol-3-yl)piperazine-1-carboxylate (73 mg) was purified by SFC under the following conditions: column, CHIRAL ART Amylose-C NEO, 3*25 cm, 5 μm; mobile phase, MeOH. This gave (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (37 mg, second peak) LCMS (ESI) m / z: [M+H] + = 667 and (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (34 mg, first peak) LCMS (ESI) m / z: [M+H] + =667 was obtained.

[0237] Step 6: Preparation of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (I-70) and (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (I-71) [ka] To a stirred solution of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (37.00 mg, 0.055 mmol, 1.00 equiv) in DCM (1.50 mL) was added HCl in 1,4-dioxane (1.50 mL, 26.276 mmol, 473.57 equiv) at 0 °C. The resulting mixture was stirred at room temperature for 1 h and then concentrated under reduced pressure. This afforded I-70 (45 mg, crude) as a yellow oil. LCMS (ESI) m / z: [M+H] + =567. I-71, a yellow oil, was prepared according to the same protocol as I-70. LCMS (ESI) m / z: [M+H] + =567.

[0238] The following intermediates in Table 7 were prepared in a similar manner as described in the preparation of intermediate I-70, starting with methyl 3-methyl-2-[3-[(1,1,2,2,3,3,4,4,4-nonafluorobutanesulfonyl)oxy]-1,2-oxazol-5-yl]butanoate and the appropriate amine. [Table 8-1] [Table 8-2]

[0239] Preparation of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-(2R)-3-methyl-2-[3-(piperidin-4-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide tert-butyl ester (I-80) [ka] Step 1: Preparation of tert-butyl 4-[(1E)-(hydroxyimino)methyl]piperidine-1-carboxylate (Intermediate 2) [ka] To a stirred solution of tert-butyl 4-formylpiperidine-1-carboxylate (5 g, 23.4 mmol, 1.00 equiv) in MeOH (10 mL) and HO (10 mL) was added hydroxylamine hydrochloride (1.95 g, 28.133 mmol, 1.2 equiv) and NaCO (1.24 g, 11.722 mmol, 0.5 equiv) at 0 degrees Celsius. The resulting mixture was stirred overnight at room temperature. The resulting mixture was concentrated under reduced pressure. The resulting mixture was extracted with EtOAc (3 × 20 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give Intermediate 2 (6 g, crude) as a colorless oil. LCMS (ESI) m / z: [M+H] + =229.

[0240] Step 2: Preparation of tert-butyl 4-[(1Z)-chloro(hydroxyimino)methyl]piperidine-1-carboxylate (Intermediate 3) [ka] A mixture of intermediate 2 and NCS (3.5 g, 26.282 mmol, 1.0 equiv) in DMF (20 mL) was stirred at room temperature for 2 hours. The desired product could be detected by LCMS. The resulting mixture was diluted with water (50.00 mL) and extracted with EtOAc (3×50 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give intermediate 3 (7.8 g, crude) as a colorless oil. LCMS (ESI) m / z [M+H] + =263.

[0241] Step 3: Preparation of tert-butyl 4-[5-(2-methoxy-2-oxoethyl)-1,2-oxazol-3-yl]piperidine-1-carboxylate (Intermediate 4) [ka] A mixture of intermediate 3 (7.8 g, crude) and NaHCO3 (3.8 g, 45.675 mmol, 1.5 equiv) in EtOAc (100 mL) was stirred for 30 minutes at room temperature. To the above mixture, methyl but-3-ynoate (2.99 g, 30.450 mmol, 1 equiv) was added at 0 degrees Celsius. The resulting mixture was stirred overnight at room temperature. The desired product could be detected by LCMS. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.05% FA) in water, gradient from 0% to 100% in 30 minutes; detector, UV 254 nm. The resulting mixture was concentrated under reduced pressure to give intermediate 4 (4.1 g, 41.51%) as a pale yellow oil. LCMS (ESI) m / z: [M+H] + =325.

[0242] Step 4: Preparation of tert-butyl 4-[5-(1-methoxy-3-methyl-1-oxobutan-2-yl)-1,2-oxazol-3-yl]piperidine-1-carboxylate (Intermediate 5) [ka] To a mixture of intermediate 4 (1.0 g, 3.083 mmol, 1.5 equiv) and NaSO (1.0 g) in THF (10 mL) was added t-BuOK (518.90 mg, 4.625 mmol, 1.5 equiv) and 2-iodopropane (628.87 mg, 3.700 mmol, 1.2 equiv) at 0°C under a dry nitrogen atmosphere. The resulting mixture was stirred at 0°C for 3 hours under a dry nitrogen atmosphere. The desired product could be detected by LCMS. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.05% FA) in water, gradient from 0% to 100% in 30 min; detector, UV 254 nm. The resulting mixture was concentrated under reduced pressure to give intermediate 5 (330 mg, 29.21%) as a pale yellow oil. LCMS(ESI)m / z:[M+H] + =367.

[0243] Step 5: Preparation of 2-{3-[1-(tert-butoxycarbonyl)piperidin-4-yl]-1,2-oxazol-5-yl}-3-methylbutanoic acid (Intermediate 6) [ka] To a stirred solution of intermediate 5 (320 mg, 0.873 mmol, 1.00 equiv) in MeOH (5 mL) was added LiOH (62.74 mg, 2.619 mmol, 3 equiv) in HO (5 mL) dropwise at room temperature. The resulting mixture was stirred at room temperature for 3 h. The desired product could be detected by LCMS. The resulting mixture was concentrated under reduced pressure. Aqueous HCl (6 M) was added to the above mixture to adjust the pH to about 5. The resulting mixture was extracted with EtOAc (3 × 50 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give intermediate 6 (crude 316 mg) as an off-white solid. LCMS (ESI) m / z: [M+H] + =353.

[0244] Step 6: Preparation of tert-butyl 4-(5-{1-[(2S,4R)-4-hydroxy-2-{[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]carbamoyl}pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl}-1,2-oxazol-3-yl)piperidine-1-carboxylate (Intermediate 7) [ka] A mixture of intermediate 6 (310 mg, 0.880 mmol, 1.00 equiv) and HATU (668.90 mg, 1.760 mmol, 2 equiv) in DMF (5 mL) was stirred at room temperature for 30 minutes. To the above mixture, (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (291.53 mg, 0.880 mmol, 1 equiv) was added at room temperature. The resulting mixture was stirred for another 2 hours at room temperature. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.05% FA) in water, 0% to 100% gradient in 30 minutes; detector, UV 254 nm. The resulting mixture was concentrated under reduced pressure to give Intermediate 7 (242 mg, 37.31%) as a light brown solid. LCMS (ESI) m / z: [M+H] + =666.

[0245] Step 7: Preparation of tert-butyl 4-{5-[(2R)-1-[(2S,4R)-4-hydroxy-2-{[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]carbamoyl}pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-1,2-oxazol-3-yl}piperidine-1-carboxylate (Intermediate 8) [ka] Intermediate 7 was purified by preparative SFC under the following conditions (column: CHIRAL ART Amylose-SA, 3*25 cm, 5 μm; mobile phase A: CO2, mobile phase B: MeOH-HPLC; flow rate: 50 mL / min; gradient: isocratic 45% B; column temperature (°C): 35; back pressure (bar): 100; wavelength: 205 nm; RT1 (min): 3.65; RT2 (min): 4.88; sample solvent: MeOH-HPLC; injection volume: 1 mL) to give intermediate 8 (second peak) (208.1 mg, 43.52%) as a light brown solid. LCMS (ESI) m / z: [M+H] + =666.

[0246] Step 8: Preparation of (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperidin-4-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide tert-butyl (I-80) [ka] To a stirred solution of intermediate 8 (200 mg, 0.300 mmol, 1.00 equiv) in DCM (2 mL) was added dropwise 1 M HCl in 1,4-dioxane (2 mL) at room temperature. The resulting mixture was stirred at room temperature for 1 h. The desired product could be detected by LCMS. The resulting mixture was concentrated under reduced pressure to give I-80 (247.5 mg) as a pale yellow solid. LCMS (ESI) m / z: [M+H] + =566.

[0247] The following intermediates in Table 8 were prepared in a similar manner as described in the preparation of intermediate I-80, starting with the appropriate aldehyde. [Table 9]

[0248] Preparation of 3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentane-1-carbaldehyde (I-87) [ka] Step 1: Preparation of 3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)-N-methoxy-N-methylbicyclo[1.1.1]pentane-1-carboxamide [ka] To a stirred mixture of 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]bicyclo[1.1.1]pentane-1-carboxylic acid (30 mg, 0.093 mmol, 1.00 equiv.) in DMF (1.0 mL), HOBT (18.92 mg, 0.140 mmol, 1.50 equiv.) and EDCI (26.85 mg, 0.140 mmol, 1.5 equiv.) were added at room temperature. After 10 min, DIEA (60.33 mg, 0.465 mmol, 5.0 equiv.) and N,O-dimethylhydroxylamine hydrochloride (27.32 mg, 0.279 mmol, 3.0 equiv.) were added to the above mixture at room temperature. The resulting mixture was stirred for an additional 2 h at room temperature. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN (0.1% FA) in water, gradient 0% to 50% in 40 min; detector, UV 254 nm. This afforded the title compound (20 mg, 52.91%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =306.

[0249] Step 2: Preparation of 3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentane-1-carbaldehyde (I-87) [ka] To a stirred solution of 3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)-N-methoxy-N-methylbicyclo[1.1.1]pentane-1-carboxamide (30 mg, 0.082 mmol, 1.00 equiv) in THF (1 mL) was added LiAlH (3.12 mg, 0.082 mmol, 1 equiv), and the mixture was stirred at 0°C for 2 h. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography using the following conditions: column, C18 silica gel; mobile phase, CHCN in water (0.05% TFA), 10% to 50% gradient in 30 min; detector, UV 254 nm, to give I-87 (22 mg, 87.52%) as a yellow solid. LCMS (ESI) m / z: [M+H] +=306.

[0250] Preparation of 3-([3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]bicyclo[1.1.1]pentan-1-yl]amino)propanoic acid (I-88) [ka] Step 1: Preparation of methyl 3-((3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentan-1-yl)amino)propanoate [ka] A solution of 2-(6-[3-aminobicyclo[1.1.1]pentan-1-yl]-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (100.00 mg, 0.342 mmol, 1.00 equiv), methyl acrylate (23.56 mg, 0.274 mmol, 0.80 equiv), and trimethylamine (103.84 mg, 1.026 mmol, 3 equiv) in methanol (2.00 mL) was stirred overnight at 60°C. Without any further processing, the residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN (0.05% FA) in water, gradient from 0% to 100% in 25 min; detector, UV 254 nm. This gave methyl 3-((3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentan-1-yl)amino)propanoate (39 mg, 30.1%) as a black oil. LCMS (ESI) m / z: [M+H] + =379.

[0251] Step 2: Preparation of 3-([3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]bicyclo[1.1.1]pentan-1-yl]amino)propanoic acid (I-88) [ka] A solution of methyl 3-((3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentan-1-yl)amino)propanoate (39.00 mg, 0.103 mmol, 1.00 equiv) and LiOH (24.68 mg, 1.031 mmol, 10.00 equiv) in THF (0.90 mL), HO (0.30 mL) was stirred for 2 h at room temperature. The reaction was monitored by LCMS. The mixture was acidified to pH 5 with concentrated hydrochloric acid. The resulting mixture was extracted with EtOAc (3 × 50 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This afforded I-88 (22 mg, 58.58%) as a yellow oil. LCMS(ESI)m / z:[M+H] + =365.

[0252] Preparation of 3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carboxylic acid (I-89) [ka] Step 1: Preparation of 3-chloro-7H-pyrrolo[2,3-c]pyridazine-6-carboxylic acid (Intermediate 2) [ka] To a solution of 4-bromo-6-chloropyridazin-3-amine (500.00 mg, 2.399 mmol, 1.00 equiv) and pyruvic acid (633.72 mg, 7.196 mmol, 3.00 equiv) in DMF (8.00 mL) was added Pd(OAc) (53.85 mg, 0.240 mmol, 0.10 equiv), DABCO (805.99 mg, 7.196 mmol, 3.00 equiv), and MgSO (250.00 mg, 2.077 mmol, 0.87 equiv). The reaction mixture was stirred at 105°C for 6 hours. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN in water, gradient from 0% to 50% in 20 minutes; detector, UV 254 nm. This gave intermediate 2 (70 mg, 14.77%) as a brown solid. LCMS (ESI) m / z: [M+H] + =198.

[0253] Step 2: Preparation of ethyl 3-chloro-7H-pyrrolo[2,3-c]pyridazine-6-carboxylate (Intermediate 3) [ka] To a solution of intermediate 2 (2 g, 10.122 mmol, 1 equiv.) in EtOH (15 mL) was added SOCl (1.81 g, 15.183 mmol, 1.5 equiv.). The resulting mixture was stirred at 50° C. for 4 h under a dry nitrogen atmosphere. The resulting mixture was concentrated in vacuo. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give intermediate 3 (1 g, 43.78%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =226.

[0254] Step 3: Preparation of ethyl 3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carboxylate (Intermediate 4) [ka] To a solution of intermediate 3 (1 g, 4.432 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (1.22 g, 8.864 mmol, 2 equiv) in dioxane (10 mL) and HO (2 mL) was added CsCO (4.33 g, 13.296 mmol, 3 equiv) and XPhos Pd G (0.38 g, 0.443 mmol, 0.1 equiv). The resulting mixture was stirred at 80 °C for 2 h under a dry nitrogen atmosphere. This afforded intermediate 4 (700 mg, 55.75%) as a brown oil. LCMS (ESI) m / z: [M+H] + =284.

[0255] Step 4: Preparation of 3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carboxylic acid (I-89) [ka] To a solution of intermediate 4 (50 mg, 0.176 mmol, 1.00 equiv) in MeOH (2 mL) was added aqueous NaOH (10 M, 0.2 mL). The reaction mixture was stirred at 60°C for 2 hours. The reaction mixture was concentrated in vacuo. The crude product was purified by preparative HPLC under the following conditions: column, XBridge Shield RP18 OBD column, 19*150 mm, 5 μm; mobile phase, water (0.05% FA) and CHCN (15% CHCN to 50% in 7 min); detector, UV 254 nm. This afforded I-89 (16.2 mg, 35.96%) as a green solid. 1 H NMR(400MHz,DMSO-d6)δ 13.27(d,J=177.8Hz,2H),8.75(s,1H),8.02(dd,J=8.0,1.6Hz,1H),7.36-7.26(m,1H),7.13-6.92(m,3H).LCMS(ESI)m / z:[M+H] + =256.05.

[0256] Preparation of tert-butyl 4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carbonyl)piperazine-1-carboxylate (I-90) [ka] Step 1: Preparation of tert-butyl 4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carbonyl)piperazine-1-carboxylate [ka] To a stirred solution of 3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carboxylic acid (60 mg, 0.235 mmol, 1.00 equiv) and tert-butyl piperazine-1-carboxylate (87.57 mg, 0.470 mmol, 2.0 equiv) in DMF (1 mL) was added EDCI (90.13 mg, 0.470 mmol, 2.0 equiv), HOBt (63.53 mg, 0.470 mmol, 2.0 equiv), and DIEA (151.91 mg, 1.175 mmol, 5.0 equiv) at room temperature. The resulting mixture was stirred at room temperature for 12 h. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN (0.1% FA) in water, gradient 0% to 100% in 25 min; detector UV 254 nm. This gave tert-butyl 4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carbonyl)piperazine-1-carboxylate (70 mg, 70.32%) as an off-white solid. LCMS (ESI) m / z [M+H] + =424.

[0257] Step 2: Preparation of 2-[6-(piperazine-1-carbonyl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol [ka] To a stirred solution of tert-butyl 4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carbonyl)piperazine-1-carboxylate (70 mg, 0.165 mmol, 1.00 equiv) in DCM (5 mL) was added TFA (1 mL) dropwise at room temperature. The resulting mixture was stirred for 2 hours at room temperature. The resulting mixture was concentrated in vacuo. This afforded 2-[6-(piperazine-1-carbonyl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (80 mg, crude) as a pale yellow oil. LCMS (ESI) m / z [M+H] + =324.

[0258] Step 3: Preparation of tert-butyl 4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine-6-carbonyl)piperazine-1-carboxylate (I-90) [ka] To a stirred solution of 2-[6-(piperazine-1-carbonyl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (70 mg, 0.216 mmol, 1.00 equiv) in THF (5 mL) was added LiAlH (16.43 mg, 0.432 mmol, 2.0 equiv) in THF (1 mL) dropwise at 0°C under a dry nitrogen atmosphere. The resulting mixture was stirred at room temperature for 4 hours under a dry nitrogen atmosphere. The reaction was quenched at room temperature by adding water (5 mL). The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN (0.05% FA) in water, gradient 0% to 100% in 30 minutes; detector, UV 254 nm. This afforded I-90 (34 mg, 35.5%) as a pale yellow solid.

[0259] Preparation of rel-(R)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylic acid (I-91) and rel-(S)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylic acid (I-92) [ka] Step 1: Preparation of tert-butyl 3-chloro-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylate (Intermediate 2) [ka] To a stirred solution of 4-bromo-6-chloropyridazin-3-amine (5256.68 mg, 25.219 mmol, 1.00 equiv) and tert-butyl 4-oxocyclohexane-1-carboxylate (5.00 g, 25.219 mmol, 1.00 equiv) in DMAc (80.00 mL) was added Pd(OAc) (1132.39 mg, 5.044 mmol, 0.20 (t-Bu)P-HBF (463.37 mg, 5.044 mmol, 0.2 equiv.), AcOH (3028.92 mg, 50.438 mmol, 2.00 equiv.), MgSO (5.00 mg, 0.042 mmol, 0.82 equiv.), and DABCO (8483.47 mg, 75.657 mmol, 3.0 equiv.) were added at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 120°C overnight under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The resulting mixture was filtered, and the filter cake was extracted with EtOAc (2 x 10 mL). The filtrate was extracted with EtOAc (3 x 500 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient 0% to 100% in 10 min; detector, UV 254 nm to give intermediate 2 (350 mg, 4.28%) as a yellow solid. LCMS (ESI) m / z: [M+H]+ = 307.78.

[0260] Step 2: Preparation of tert-butyl 3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylate (Intermediate 3) [ka] To a solution of Intermediate 2 (610 mg, 1.982 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (410.05 mg, 2.973 mmol, 1.5 equiv) in 1,4-dioxane (10.00 mL) and HO (2.00 mL) was added CsCO (1291.51 mg, 3.964 mmol, 2.0 equiv) and XPhos Pd G (167.76 mg, 0.198 mmol, 0.1 equiv). The resulting mixture was stirred at 80°C under a dry nitrogen atmosphere overnight. The resulting mixture was concentrated under reduced pressure. The residue was dissolved in DMF and purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient 0% to 100% in 20 min; detector, UV 254 nm to give intermediate 3 (450 mg, 59.65%) as a yellow solid. LCMS (ESI) m / z: [M+H]+ = 365.43.

[0261] Step 3: Preparation of rel-(R)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylic acid (intermediate 4a) and (S)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylate tert-butyl ester (intermediate 4b) [ka] Intermediate 3 (680 mg) was purified by SFC under the following conditions: (Column: CHIRALPAK ID, 3 × 25 cm, 5 μm; Mobile phase A: CO₂, Mobile phase B: EtOH - HPLC; Flow rate: 50 mL / min; Gradient: Isocratic 55% B; Column temperature (°C): 35; Back pressure (bar): 100; Wavelength: 217 nm; RT1 (min): 5.1; RT2 (min): 7.55; Sample solvent: MeOH:DCM = 1:1; Injection volume: 8 mL; Run number: 9) to give Intermediate 4a (233 mg) and Intermediate 4b (246 mg) as brown solids. LCMS (ESI) m / z: [M+H]+ = 365.43.

[0262] Step 4: Preparation of rel-(R)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylic acid (I-91) and rel-(S)-3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indole-6-carboxylic acid (I-92) [ka] To a stirred solution of intermediate 4a (233.00 mg, 0.638 mmol, 1.00 equiv) in DCM (12.00 mL) was added TFA (4.00 mL, 53.852 mmol, 84.46 equiv) at room temperature. The resulting mixture was stirred at room temperature for 4 h. The resulting mixture was concentrated under reduced pressure. The residue was dissolved in ACN and water (50.00 mL) and lyophilized to give I-91 (170 mg, 79.04%) as a yellow solid. LCMS (ESI) m / z: [M+H]+ = 309.33. I-92 (190 mg, 89.42%) as a yellow solid was obtained using the same protocol starting from intermediate 4b. LCMS (ESI) m / z: [M+H]+ = 309.33.

[0263] Preparation of (R)-2-(6-amino-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-3-yl)phenol and (S)-2-(6-amino-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-3-yl)phenol (I-93 and I-94) [ka] Yellow solids I-93 and I-94 were prepared following a similar procedure to I-91 and I-92, starting from 4-bromo-6-chloropyridazin-3-amine and tert-butyl N-(4-oxocyclohexyl)carbamate. LCMS (ESI) m / z: [M+H] + =281.

[0264] Preparation of (S)-2-(6-(methylamino)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-3-yl)phenol (I-95) [ka] Step 1: Preparation of (S)-(3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-6-yl)carbamate tert-butyl [ka] A mixture of 2-[(6S)-6-amino-5H,6H,7H,8H,9H-pyridazino[3,4-b]indol-3-yl]phenol (50.0 mg, 0.178 mmol, 1.00 equiv.), (Boc)O (38.9 mg, 0.178 mmol, 1.00 equiv.), and NaHCO (29.9 mg, 0.356 mmol, 2.00 equiv.) in THF (1 mL) and HO (1 mL) was stirred at room temperature for 3 h. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, gradient from 0% to 100% in 25 min; detector, UV 254 and 220 nm. The resulting mixture was concentrated under reduced pressure to give tert-butyl (S)-(3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-6-yl)carbamate (40 mg, 58.95%) as a yellow solid. LCMS (ESI) m / z: [M+H] +=381.

[0265] Step 2: Preparation of (S)-2-(6-(methylamino)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-3-yl)phenol (I-95) [ka] A mixture of tert-butyl (S)-(3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyridazino[3,4-b]indol-6-yl)carbamate (40.0 mg, 0.105 mmol, 1.00 equiv) and LiAlH (7.9 mg, 0.210 mmol, 2.00 equiv) in THF (2 mL) was stirred at 0° C. for 5 minutes under a dry nitrogen atmosphere. The resulting mixture was stirred at 0° C. for 1 hour under a dry nitrogen atmosphere, allowed to warm to room temperature, and stirred for 2 hours. The desired product could be detected by LCMS. The reaction was quenched by adding NaSO.10HO at 0° C. The precipitated solid was collected by filtration and washed with MeCN (3×30 mL). The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, gradient 0% to 100% in 20 min; detector, UV 254 and 220 nm. The resulting mixture was concentrated under reduced pressure to give I-95 (15 mg, 48.47%) as an off-white solid. LCMS (ESI) m / z: [M+H] + =295.

[0266] Preparation of 2-{5',7'-dihydrospiro[azetidine-3,6'-pyrrolo[2,3-c]pyridazin]-3'-yl}phenol (I-96) [ka] Step 1: Preparation of benzyl 3-[(2-methylpropane-2-sulfinyl)imino]azetidine-1-carboxylate (Intermediate 2) [ka] To a stirred solution of benzyl 3-oxoazetidine-1-carboxylate (4 g, 19.492 mmol, 1.00 equiv.) and tert-butanesulfinamide (2.36 g, 19.492 mmol, 1 equiv.) in THF (40 mL) was added Ti(Oi-Pr) (5.54 g, 19.492 mmol, 1 equiv.). The resulting mixture was stirred at 60°C for 1 hour. The resulting mixture was diluted with EtOAc (300 mL) and washed with water (100 mL x 3), and the organic layer was dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give intermediate 2 (3.58 g, 59.55%) as a white solid. LCMS (ESI) m / z: [M+H] + =309.

[0267] Step 2: Preparation of benzyl 3-[(2-methylpropane-2-sulfinyl)amino]-3-[3-(trimethylsilyl)prop-2-yn-1-yl]azetidine-1-carboxylate (Intermediate 3) [ka] A mixture of intermediate 2 (1.5 g, 4.864 mmol, 1.00 equiv), (3-bromoprop-1-yn-1-yl)trimethylsilane (2.79 g, 14.592 mmol, 3 equiv), and Zn (0.95 g, 14.592 mmol, 3 equiv) in THF (20 mL) was stirred at 25°C for 16 hours. The mixture was diluted with EtOAc (80 mL) and washed with water (80 mL x 3). The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to give the crude product. The crude product was purified by flash C18 chromatography, eluting with a gradient of 0 to 65% ACN in HO to give intermediate 3 (1.9 g, 92.91%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =421.

[0268] Step 3: Preparation of benzyl 3-[(2-methylpropane-2-sulfinyl)amino]-3-(prop-2-yn-1-yl)azetidine-1-carboxylate (Intermediate 4) [ka] To a stirred solution of intermediate 3 (1.5 g, 3.566 mmol, 1.00 equiv) in DCM (20 mL) was added TBAF (17.83 mL, 17.830 mmol, 5 equiv). The resulting mixture was stirred for 1 h at room temperature. The resulting mixture was diluted with DCM (100 mL). The resulting mixture was washed with 5 x 50 mL of 5% HCl (aq). The organic layer was concentrated in vacuo. The crude product was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =349.

[0269] Step 4: Preparation of benzyl 3-amino-3-(prop-2-yn-1-yl)azetidine-1-carboxylate (Intermediate 5) [ka] To a stirred solution of intermediate 4 (600 mg, 1.722 mmol, 1.00 equiv) in DCM (10 mL) was added 4 M HCl in MeOH (5 mL). The resulting mixture was stirred for 30 minutes at room temperature. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography using the following conditions: column, C18 silica gel; mobile phase, MeOH in water, 10% to 50% gradient over 30 minutes; detector, UV 200 nm to give intermediate 5 (310 mg, 73.70%). LCMS (ESI) m / z: [M+H] + =245.

[0270] Step 5: Preparation of benzyl 3'-chloro-5',7'-dihydrospiro[azetidine-3,6'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (Intermediate 6) [ka] To a stirred solution of intermediate 5 (300 mg, 1.228 mmol, 1.00 equiv) and DIEA (634.86 mg, 4.912 mmol, 4 equiv) in dioxane (5 mL) was added dichloro-1,2,4,5-tetrazine (370.74 mg, 2.456 mmol, 2 equiv). The resulting mixture was stirred at 100°C for 2 hours. The resulting mixture was concentrated in vacuo. The residue was purified by silica gel column chromatography eluting with CHCl / MeOH (10:1) to give intermediate 6 (220 mg, 54.16%) as a brown solid. LCMS (ESI) m / z: [M+H] + =331.

[0271] Step 6: Preparation of benzyl 3'-(2-hydroxyphenyl)-5',7'-dihydrospiro[azetidine-3,6'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (Intermediate 7) [ka] To a stirred solution of intermediate 6 (200 mg, 0.605 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (250.20 mg, 1.815 mmol, 3 equiv) in dioxane (5 mL) and HO (1 mL) was added XPhos Pd G3 (102.36 mg, 0.121 mmol, 0.2 equiv) and K2CO3 (250.70 mg, 1.815 mmol, 3 equiv). The resulting mixture was stirred at 90°C for 2 h under a dry nitrogen atmosphere. The resulting mixture was diluted with EtOAc (300 mL) and washed with water (100 mL × 3), and the organic layer was dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with CHCl2 / MeOH (10:1) to give intermediate 7 (121 mg, 51.52%) as a brown solid. LCMS(ESI)m / z:[M+H] + =389.

[0272] Step 7: Preparation of 2-{5',7'-dihydrospiro[azetidine-3,6'-pyrrolo[2,3-c]pyridazin]-3'-yl}phenol) (I-96) [ka] A stirred solution of intermediate 7 (20 mg, 0.051 mmol, 1.00 equiv) and Pd / C (10.96 mg, 0.102 mmol, 2 equiv) in MeOH (3 mL) was stirred under a hydrogen atmosphere for 2 h at room temperature. The resulting mixture was filtered, and the filter cake was washed with MeOH (3 × 10 mL). The filtrate was concentrated under reduced pressure. The crude product (25 mg) was purified by preparative HPLC under the following conditions (column: XBridge Prep C18 OBD column, 19 × 150 mm, 5 μm; mobile phase A: water (0.05% FA) mobile phase B: CH3CN; flow rate: 25 mL / min; gradient: 4% B to 26% B, 26% B in 8 min; wavelength: 254 / 220 nm) to give I-96 (5 mg, 38%) as a brown solid. 1 H NMR(400MHz,DMSO-d6)δ 14.19(s,1H),8.30(s,1H),8.06(s,1H),7.84(d,J=7.8Hz,1H),7.25(t,J=7.7Hz,1H),6.90(d, J=7.9Hz,2H),4.02(s,1H),3.76(d,J=7.4Hz,2H),3.46(d,J=23.1Hz,4H).LCMS(ESI)m / z:[M+H] + =255.25.

[0273] Preparation of (1S,3R)-3'-(2-hydroxyphenyl)-5',7'-dihydrospiro[cyclobutane-1,6'-pyrrolo[2,3-c]pyridazine]-3-carboxylic acid (I-97) and (1R,3S)-3'-(2-hydroxyphenyl)-5',7'-dihydrospiro[cyclobutane-1,6'-pyrrolo[2,3-c]pyridazine]-3-carboxylic acid (I-98) [ka] Yellow solids I-97 and I-98 were obtained following a similar procedure to I-96, starting from ethyl 3-oxocyclobutane-1-carboxylate and tert-butanesulfinamide. LCMS (ESI) m / z: [M+H] + =298.

[0274] Preparation of 1-(3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)azetidin-1-yl)prop-2-en-1-one (I-99) [ka] To a mixture of acrylic acid (8.1 mg, 0.113 mmol, 1.00 equiv) in DMF (1 mL) was added HATU (64.2 mg, 0.170 mmol, 1.50 equiv) and stirred for 20 minutes. 2-[6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (30.0 mg, 0.113 mmol, 1.00 equiv) and DIEA (43.6 mg, 0.339 mmol, 3.00 equiv) were added. The mixture was stirred for 2 hours at room temperature. The crude reaction mixture was purified by preparative HPLC under the following conditions: Column: XBridge Prep C 18 Purification on an OBD column, 19*150 mm, 5 μm; mobile phase A: water (10 mmol / L NH4HCO3), mobile phase B: ACN; flow rate: 25 mL / min; gradient: 28% B to 37% B, 37% B in 10 min; wavelength: 254 / 220 nm gave I-99 (3.4 mg, 8.6%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =321.35.

[0275] Preparation of 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (I-6) [ka] Step 1: Preparation of tert-butyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate [ka] To a stirred mixture of (2S,4R)-1-[(2S)-2-amino-3,3-dimethylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide hydrochloride (400 mg, 0.832 mmol) in DMF (5.00 mL) at room temperature, 10-(tert-butoxy)-10-oxodecanoic acid (215 mg, 0.832 mmol), DIEA (322 mg, 2.50 mmol), and HATU (474 mg, 1.25 mmol) were added. The resulting mixture was stirred at room temperature for 2 hours. The residue was purified by reverse-phase C18 flash chromatography (water:ACN:FA) to give tert-butyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate (440 mg, 65%) as an off-white solid. LCMS (ESI) m / z [M+H] + =685.

[0276] Step 2: Preparation of 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (I-6) [ka] To a stirred mixture of tert-butyl 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoate (430 mg, 0.628 mmol) in DCM (5.00 mL) was added TFA (1.50 mL) at room temperature. The resulting mixture was stirred at room temperature for 1 hour. The residue was purified by reverse-phase C18 flash chromatography (water:ACN:FA) to give 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (I-6, 280 mg, 70.8%). LCMS (ESI) m / z [M+H] + =629.

[0277] The following intermediates in Table 9 were prepared in a manner similar to that described for the preparation of intermediate I-6 using (2S,4R)-1-[(2S)-2-amino-3,3-dimethylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide hydrochloride and the appropriate carboxylic acid. [Table 10]

[0278] Example 2 Preparation of (2S,4R)-4-hydroxy-1-((S)-2-(10-(3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)azetidin-1-yl)-10-oxodecanamido)-3,3-dimethylbutanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (Compound 1) [ka] To a stirred mixture of 2-(6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-1, 8.47 mg, 0.032 mmol) and DIEA (12.3 mg, 0.095 mmol) in DMF (2 mL) at room temperature, 10-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-10-oxodecanoic acid (20.0 mg, 0.032 mmol) and HATU (18.1 mg, 0.048 mmol) were added. The resulting mixture was stirred at room temperature overnight. The crude product was purified by preparative HPLC (water:ACN:FA) to give compound 1 (7 mg, 98.2%) as an off-white solid. 1 H NMR(300MHz,DMSO-d6)δ 13.01(br s,1H),8.99(s,1H),8.61(s,1H),8.38(d,J=7.8Hz,1H),7.91(s,1H),7.79(d,J=9.3Hz,1H),7.47-7. 32(m,5H),7.06-6.96(m,2H),6.79(s,1H),4.99-4.84(m,1H),4.63-4.48(m,2H),4.47-4.37(m,2H),4 .37-4.16(m,3H),4.12-4.03(m,1H),3.60(s,2H),2.46(s,3H),2.33-2.19(m,1H),2.18-1.96(m,4H), 1.88-1.72(m,1H),1.58-1.42(m,4H),1.38(d,J=7.0Hz,3H),1.26(s,8H),0.94(s,9H).LCMS(ESI)m / z [M+H] + =877.20.

[0279] The compounds in Table 10 were prepared using procedures similar to those used for the preparation of Compound 1, using the appropriate amine and carboxylic acid OR. [Table 11-1] [Table 11-2] [Table 11-3] [Table 11-4]

[0280] Example 3 Preparation of (2S,4R)-4-hydroxy-1-((R)-2-(3-(2-(3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)azetidin-1-yl)ethoxy)isoxazol-5-yl)-3-methylbutanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamidoformate (Compound 14) [ka] To a solution of 2-(6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-1, 6.60 mg, 0.025 mmol) and (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(2-oxoethoxy)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (13.4 mg, 0.025 mmol) in a mixture of MeOH (2.00 mL) and DCM (2.00 mL) was added AcOH (0.10 mL, 1.75 mmol) until the pH reached 6. Then, NaBHCN (6.23 mg, 0.100 mmol) was added to the reaction mixture, and the mixture was stirred at room temperature for 10 hours. The mixture solution was purified by preparative HPLC to give compound 14 (10 mg, 47.0%) as a white solid. 1H NMR(400MHz,DMSO-d6)δ 14.01(s,1H),12.66(s,1H),8.99(s,1H),8.61(s,1H),8.41(d,J=7.9Hz,1H),8.14(d,J=1.2Hz,1H),8.05(d,J=7.8Hz,1H),7.44(d,J=7 .9Hz,2H),7.36(d,J=8.1Hz,2H),7.30(t,J=7.5Hz,1H),6.96(t,J=8.3Hz,2H),6.66(s,1H),6.13(s,1H),5.12(d,J=3.5Hz,1H),4.92(q, J=7.1Hz,1H),4.36(t,J=7.9Hz,6H),4.08(s,2H),3.74-3.64(m,3H),2.48(s,3H),2.30-2.18(m,1H),2.04(dd,J=19.4,8.4Hz,1H),1.7 8(dd,J=12.7,8.2,Hz,1H),1.5(s,1H),1.37(d,J=7.0Hz,3H),0.97(t,J=6.0Hz,4H),0.82(dd,J=14.1,6.7Hz,4H).LCMS(ESI)m / z:[M+H] + =791.3.

[0281] Example 4 Preparation of (2S,4R)-4-hydroxy-1-((R)-2-(3-(2-(3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)azetidin-1-yl)ethoxy)isoxazol-5-yl)-3-methylbutanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamidoformate (Compound 15) [ka] Compound 15 was prepared according to the synthetic procedure described for the preparation of compound 14, starting from 2-(5-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-2) and (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(2-oxoethoxy)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide. 1 H NMR(400MHz,DMSO-d6)δ 14.01(s,1H),12.51(s,1H),8.98(s,1H),8.81(s,1H),8.40(d,J=7.7H z,1H),8.11-8.03(m,2H),7.47-7.42(m,2H),7.39-7.34(m,2H),7.30( td,J=7.6,1.6Hz,1H),7.02-6.93(m,2H),6.10(s,1H),5.10(d,J=3.6Hz,1H),4.90(q,J=7.2Hz,1H),4.36(t,J=7.9Hz,1H),4.31-4.22(m,3H) ,3.99(s,3H),3.71(dd,1H),3.66(d,J=9.7Hz,1H),3.55(s,1H),3.45(d,J=10.9Hz,2H),3.06(s,2H),2.45(d,J=2.6Hz,3H),2.24(d,J=9.7Hz) ,1H),2.07-1.97(m,1H),1.83-1.74(m,1H),1.37(d,J=7.0Hz,3H),0.95(d,J=6.4Hz,3H),0.80(dd,J=13.7,6.7Hz,3H).LCMS(ESI)m / z:[M+H] + =791.35.

[0282] The compounds in Table 11 were prepared according to the following protocol (Procedure B) similar to that described above for Compound 15 using the appropriate amine and aldehyde (or ketone), or according to a procedure (Procedure A) similar to that described above for Compound 1 using the appropriate amine and carboxylic acid. [Table 12-1]

Table 12-2

Table 12-3

Table 12-4

Table 12-5

Table 12-6

Table 12-7

Table 12-8

Table 12-9

Table 12-10

Table 12-11

Table 12-12

Table 12-13

Table 12-14

Table 12-15

Table 12-16

Table 12-17

Table 12-18

Table 12-19

Table 12-20

Table 12-21

Table 12-22

Table 12-23

Table 12-24

Table 12-25

Table 12-26

Table 12-27

Table 12-28

Table 12-29

Table 12-30

Table 12-31

Table 12-32

Table 12-33

Table 12-34

Table 12-35

Table 12-36

Table 12-37

Table 12-38

Table 12-39

Table 12-40

Table 12-41

Table 12-42

Table 12-43

Table 12-44

Table 12-45

Table 12-46

Table 12-47

Table 12-48

Table 12-49

Table 12-50

Table 12-51

Table 12-52

Table 12-53

Table 12-54

Table 12-55

Table 12-56

Table 12-57

Table 12-58

Table 12-59

Table 12-60

Table 12-61

Table 12-62

Table 12-63

Table 12-64

Table 12-65

Table 12-66

Table 12-67

Table 12-68

Table 12-69

Table 12-70

Table 12-71

Table 12-72

Table 12-73

Table 12-74

Table 12-75

Table 12-76

Table 12-77

Table 12-78

Table 12-79

Table 12-80

Table 12-81

Table 12-82

Table 12-83

[0283] In Table 11, the procedure column lists compounds prepared from the appropriate amine and carboxylic acid as "A" and compounds prepared from the appropriate amine and aldehyde (or ketone) as "B."

[0284] Preparation of (2S,4R)-4-hydroxy-1-[(2S)-2-[3-([3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]bicyclo[1.1.1]pentan-1-yl](methyl)amino)propanamido]-3,3-dimethylbutanoyl]-N-[[4-(4-methyl-1,3-thiazol-5-yl)phenyl]methyl]pyrrolidine-2-carboxamide (Compound 293) [ka] (2S,4R)-4-hydroxy-1-((S)-2-(3-((3-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl)bicyclo[1.1.1]pentan-1-yl)amino)propanamido)-3,3-dimethylbutanoyl)-N-(4-(4-methylthiazolinone)) in DCM (1.00 mL) and MeOH (1.00 mL) A solution of (5-yl)benzyl)pyrrolidine-2-carboxamide (40.00 mg, 0.051 mmol, 1.00 equiv), acetic acid (15.46 mg, 0.257 mmol, 5 equiv), formaldehyde (7.73 mg, 0.257 mmol, 5.00 equiv), and NaBHCN (16.18 mg, 0.257 mmol, 5 equiv) was stirred for 2 hours at room temperature. The reaction was monitored by LCMS. The residue was purified by column: XBridge Shield RP18 OBD column, 19*150 mm, 5 μm; mobile phase A: water (10 mmol / L NHHCO), mobile phase B: CHCN; flow rate: 25 mL / min; gradient: 42% B to 48% B in 8 min, UV: 254 / 220 nm. This gave compound 293 (2.8 mg, 6.88%) as a white solid. 1H NMR(400MHz,DMSO-d6)δ 14.14(s,1H),8.93(s,1H),8.58(t,J=6.0Hz,1H),8.51(s,1H),8.27(d,J=9.4Hz,1H),8.02(dd,J=8.1,1.7Hz,1H),7.43( d,J=8.3Hz,2H),7.37(d,J=8.3Hz,2H),7.29(ddd,J=8.4,7.2,1.5Hz,1H),7.00-6.91(m,2H),6.36(s,1H),5.14(s,1H),4. 56(d,J=9.4Hz,1H),4.49-4.39(m,2H),4.36(s,1H),4.21(dd,J=16.0,5.5Hz,1H),3.66(t,J=9.4Hz,2H),2.70-2.55(m,1H) ),2.54(s,2H),2.41(s,3H),2.23(s,3H),2.19(s,6H),2.04(s,1H),1.96-1.85(m,1H),0.96(s,9H).LCMS(ESI)m / z:[M+H] + =791.15.

[0285] The compounds in Table 12 were prepared according to the following protocol similar to that described above for compound 293 using the appropriate amine and formaldehyde. [Table 13-1] [Table 13-2]

[0286] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-{3-[4-(3-{3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidin-1-yl}-3-oxopropyl)piperazin-1-yl]-1,2-oxazol-5-yl}-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 268) [ka] To a stirred solution of (2S,4R)-4-hydroxy-N-[(1R)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2S)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (15 mg, 0.026 mmol, 1.00 equiv) and 1-{3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidin-1-yl}prop-2-en-1-one (8.48 mg, 0.026 mmol, 1.0 equiv) in MeOH (2 mL) was added DIEA (17.10 mg, 0.130 mmol, 5.0 equiv) dropwise at room temperature. The resulting mixture was stirred at 60°C for 12 hours. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography using the following conditions: column, C18 silica gel; mobile phase, MeOH (0.1% TFA) in water, gradient from 10% to 100% in 25 minutes; detector, UV 254 nm. This gave compound 268 as an off-white solid. 1H NMR (400 MHz, DMSO-d6) δ 14.21(s,1H),8.99(d,J=2.1Hz,1H),8.56(s,1H),8.39(d,J=7.7Hz,1H),8.03(d d,J=8.1,1.7Hz,1H),7.49-7.40(m,2H),7.37(d,J=8.1Hz,2H),7.33-7.24(m,1H ),6.95(t,J=8.9Hz,2H),6.64(s,1H),6.15(s,1H),5.10(s,1H),4.91(t,J=7.2H z,1H),4.59(t,J=8.6Hz,1H),4.42-4.33(m,2H),4.28(d,J=8.5Hz,2H),4.21-4.1 1(m,1H),4.11-4.03(m,1H),3.71(dd,J=10.5,4.4Hz,1H),3.57(d,J=9.9Hz,1H) ,3.42(d,J=10.1Hz,1H),3.16(d,J=5.4Hz,4H),2.60(d,J=7.5Hz,2H),2.48-2.4 2(m,6H),2.33-2.13(m,3H),2.02(t,J=10.2Hz,1H),1.84-1.73(m,1H),1.38(d, J=7.0Hz,3H),0.95(d,J=6.6Hz,3H),0.78(d,J=6.7Hz,3H).LCMS(ESI)m / z:[M+H] + =887.30.

[0287] The compounds in Table 13 were prepared according to the following protocol similar to that described above for compound 268 using the appropriate acrylamide and amine. [Table 14]

[0288] Preparation of (R)-2-(5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-100) and (S)-2-(5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-101) [ka] Step 1: Preparation of tris(tert-butyl N-[4-(3-amino-6-chloropyridazin-4-yl)but-3-yn-1-yl]carbamate) (Intermediate 2) [ka] To a stirred solution of tris(tert-butyl N-(but-3-yn-1-yl)carbamate) (10 g, 19.698 mmol, 1 equiv.) and 4-bromo-6-chloropyridazin-3-amine (15.81 g, 75.837 mmol, 3.85 equiv.) in DMF, Pd(dppf)Cl (4.76 g, 6.500 mmol, 0.33 equiv.) was added at room temperature under a dry nitrogen atmosphere. To the above mixture, CuI (2.25 g, 11.814 mmol, 0.60 equiv.) and EtN (40.00 mL, 287.788 mmol, 14.61 equiv.) were added at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 60 °C for an additional 3 h. The mixture was then diluted with water (500 mL) and extracted with EtOAc (600 mL). The organic layer was washed with brine (2 x 250 mL), then dried over anhydrous sodium sulfate, filtered, and concentrated to give the crude product, which was purified by chromatography on silica gel eluting with 10 / 1 to 1 / 1 PE / EtOAc to give intermediate 2 (8.7 g, 49%) as a brown solid. LCMS (ESI) m / z: [M+H] + =296.9.

[0289] Step 2: Preparation of tert-butyl N-(2-{3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl}ethyl)carbamate (Intermediate 3) [ka] To a stirred solution of intermediate 2 (8.7 g, 2.247 mmol, 1 equiv) in THF was added t-BuOK (4.5 g, 40.102 mmol, 17.85 equiv). The resulting mixture was stirred at 0-25 °C for 2 h under a dry nitrogen atmosphere. The reaction was quenched with saturated NH4Cl (aq) (50 mL) at 0 °C, then diluted with water (300 mL x 2) and extracted with EtOAc (700 mL). The organic layer was washed with brine (150 mL x 2), dried over anhydrous Na2SO4, and concentrated under reduced pressure to give the crude product. The residue was purified by chromatography on silica gel eluting with PE / EtOAc = 1 / 1 to give intermediate 3 (6.6 g, 75.86%) as a brown solid. LCMS (ESI) m / z: [M+H] + =296.9.

[0290] Step 3: Preparation of 2-{3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl}ethanamine (Intermediate 4) [ka] To a stirred solution of intermediate 3 (6.6 g, 22.240 mmol, 1 equiv) in THF (80 mL) was added TsOH (7659.52 mg, 44.480 mmol, 2.00 equiv) at room temperature. The resulting mixture was stirred at 70 °C for 2 h. The resulting mixture was filtered and concentrated under reduced pressure to give intermediate 4 (5.5 g, crude) as a brown solid. LCMS (ESI) m / z: [M+H] + =197.1.

[0291] Step 4: Preparation of 3-chloro-5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazine (Intermediate 5) [ka] To a stirred solution of intermediate 4 (5.5 g, 27.970 mmol, 1 equiv) and acetaldehyde (2.46 g, 55.940 mmol, 2.00 equiv) in water was added HO (75 mL) and NaOH (1 M, 50 mL). The resulting mixture was stirred for 12 h at 70 °C. The residual product was purified by reverse-phase flash chromatography using the following conditions (0.04% NH OH) to give intermediate 5 (2.5 g, 40.14%) as a brown solid. LCMS (ESI) m / z: [M+H] + =223.1.

[0292] Step 5: Preparation of 3-(2-(methoxymethoxy)phenyl)-5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazine (Intermediate 6) [ka] A stirred solution of intermediate 5 (2.5 g, 11.227 mmol, 1 equiv.), 2-(methoxymethoxy)phenylboronic acid (4.65 g, 25.597 mmol, 2.28 equiv.), XPhos Pd G3 (1.90 g, 2.245 mmol, 0.20 equiv.), and Cs2CO3 (10.97 g, 33.681 mmol, 3 equiv.) in 1,4-dioxane (35 mL) and water (7 mL) was degassed and purged with N2 three times. The resulting mixture was stirred at 80 °C for 2 h under a dry nitrogen atmosphere. The mixture was then diluted with water (120 mL) and extracted with EtOAc (120 mL). The organic layer was washed with brine (2 x 60 mL), dried over anhydrous sodium sulfate, filtered, and concentrated to give the crude product, which was purified by reverse-phase flash chromatography under the following conditions (0.1% FA) to give intermediate 6 (500 mg, 13.73%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =324.

[0293] Step 6: Preparation of 2-(5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (Intermediate 7) [ka] To a stirred solution of intermediate 6 (500 mg, 1.541 mmol, 1 equiv) in MeOH (10 mL) was added HCl (4.00 mL, 4 M in MeOH). The resulting mixture was stirred for 24 hours at room temperature. The mixture was concentrated to give intermediate 7 (320 mg, 74.06%) as an orange solid. LCMS (ESI) m / z: [M+H] + =280.

[0294] Step 7: Preparation of (R)-2-(5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-100) and (S)-2-(5-methyl-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-101) [ka] Intermediate 7 (250 mg) was purified by SFC using the following conditions: N-CHIRALPAK AD-3 (Lot No. AD3SCK-SB00113.0*100 mm, 3.0 μm); Mobile phase B: MeOH (0.1% DEA); Detector: UV 254 / 220 nm. This gave I-100 (85 mg, 19.45%) as a brown solid. 1 H NMR(400MHz,DMSO-d6)δ 14.37(s,1H),12.3(brs,1H),8.47(s,1H),8.19-8.10(m,1H),7.28(td,J=7.5,1.6Hz,1H),7.02-6.86(m,2H),4.13(d,J=6.6H) z,1H),3.25-3.17(m,1H),2.93(ddd,J=12.5,7.8,4.8Hz,1H),2.85-2.65(m,2H),1.49(d,J=6.6Hz,3H).LCMS(ESI)m / z:[M+H] + =280.

[0295] I-101 (58 mg, 13.27%) was obtained as a brown solid. 1H NMR(400MHz,DMSO-d6)δ 14.37(s,1H),12.3(brs,1H),8.47(s,1H),8.18-8.12(m,1H),7.28(td,J=7.6,1.6Hz,1H),7.00-6.90(m,2H),4.12(d,J=6.7Hz,1H) ),3.21(dt,J=12.2,4.8Hz,1H),2.92(ddd,J=12.5,7.8,4.7Hz,1H),2.86-2.66(m,2H),1.48(d,J=6.6Hz,3H).LCMS(ESI)m / z:[M+H] + =280.

[0296] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(4-{[(3R)-12-(2-hydroxyphenyl)-3-methyl-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(13),2(7),9,11-tetraen-4-yl]methyl}piperidin-1-yl)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 81) [ka] A solution of I-100 (11.80 mg, 0.042 mmol, 1 equiv.) and I-74 (25 mg, 0.042 mmol, 1 equiv.) in DCM (0.5 mL) / MeOH (0.5 mL) / AcOH (catalytic) was stirred for 30 min at room temperature. NaBHCN (13.23 mg, 0.210 mmol, 5 equiv.) was then added, and the reaction was stirred for 2 h. The crude product was purified by preparative HPLC (Column: XBridge Shield RP18 OBD column, 19*150 mm, 5 μm; Mobile phase A: water (10 mmol / L NHHCO), Mobile phase B: ACN; Flow rate: 25 mL / min; Gradient: 45% B to 63% B, 63% B in 7 min; Wavelength: 254 / 220 nm) to give compound 81 (1.2 mg, 3.33%) as a white solid. 1H NMR(400MHz, methanol-d4)δ 8.87(s,1H),8.36(s,1H),8.01-7.94(m,1H),7.48-7.34(m,4H),7.28(t,J=7.7Hz,1H),7.01-6.93(m,2H),6.10(s,1H),4.58(s,2H),4. 51(t,J=8.2Hz,1H),4.44(s,1H),4.12-4.00(m,1H),3.84(dd,J=10.8,4.2Hz,1H),3.70(s,3H),3.66-3.57(m,3H),2.99(s,1H),2.92(s, 1H),2.89(s,3H),2.81(d,J=17.2Hz,1H),2.61(dd,J=12.6,6.7Hz,1H),2.55-2.45(m,1H),2.48(s,3H),2.35(s,1H),2.17(s,1H),2.01 -1.85(m,3H),1.58(d,J=7.0Hz,1H),1.55-1.41(m,8H),1.29(s,6H),1.05(d,J=6.6Hz,4H),0.90(dd,J=11.9,6.7Hz,4H).LCMS(ESI)m / z [M+H] + =858.4.

[0297] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(4-{[(3S)-12-(2-hydroxyphenyl)-3-methyl-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(13),2(7),9,11-tetraen-4-yl]methyl}piperidin-1-yl)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 83) [ka] Compound 83 (1.5 mg, white solid) was prepared following the synthetic procedure described for the preparation of compound 81, starting from I-101 and I-74. 1H NMR(400MHz, methanol-d4)δ 8.87(s,1H),8.36(s,1H),7.97(dd,J=8.3,1.5Hz,1H),7.59-7.35(m,4H),7.28(t,J=7.1Hz,1H),7.06-6.92(m,2H),6.07(d,J= 22.7Hz,1H),4.51(t,J=8.2Hz,1H),4.44(s,1H),4.09(d,J=6.7Hz,1H),3.84(dd,J=10.8,4.1Hz,1H),3.70(s,2H),3.66-3.48(m ,3H),3.12-3.05(m,1H),3.01(d,J=17.2Hz,1H),2.86(q,J=18.8,16.4Hz,4H),2.62(dd,J=19.4,6.7Hz,1H),2.47(d,J=2.8Hz,4 LCMS(ESI)m / z [M+H] + =858.4.

[0298] The compounds in Table 14 were prepared using procedures similar to those used above for the preparation of Compound 1, using the appropriate amine and carboxylic acid. [Table 15]

[0299] Preparation of (2S,4R)-4-hydroxy-1-((R)-2-(3-(1-(3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazine-6-carbonyl)piperidin-4-yl)isoxazol-5-yl)-3-methylbutanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (Compound 105) [ka] Step 1: Preparation of 4-nitrophenyl 3-(2-hydroxyphenyl)-5,7,8,9-tetrahydro-6H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazine-6-carboxylate (Intermediate 2) [ka] To a stirred solution of 2-(6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (100 mg, 0.376 mmol, 1 equiv.) and 4-nitrophenyl carbonochloridate (75.7 mg, 0.376 mmol, 1 equiv.) in dichloromethane, NMM (75.9 mg, 0.752 mmol, 2 equiv.) was added dropwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at room temperature for 12 hours under a dry nitrogen atmosphere. The resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with CHCl / MeOH (10:1) to give intermediate 2 (52 mg, 32%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =432.

[0300] Step 2: Preparation of (2S,4R)-4-hydroxy-1-((R)-2-(3-(1-(3-(2-hydroxyphenyl)-6,7,8,9-tetrahydro-5H-pyrido[3',4':4,5]pyrrolo[2,3-c]pyridazine-6-carbonyl)piperidin-4-yl)isoxazol-5-yl)-3-methylbutanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (compound 105) [ka] A mixture of Intermediate 2 (20 mg, 0.046 mmol, 1 equiv.), (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperidin-4-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (26.23 mg, 0.046 mmol, 1 equiv.), and DMAP (catalytic) in CHCN (0.5 mL) was stirred at 80° C. for 48 hours under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature and then concentrated in vacuo. The crude product was purified by preparative HPLC (column: XBridge Shield RP18 OBD column, 19*150 mm, 5 μm; mobile phase A: water (10 mmol / L NH4HCO3), mobile phase B: ACN (0.1% FA); flow rate: 25 mL / min; gradient: 35% B to 56% B in 6 min; wavelength: 254 / 220 nm) to give compound 105 (4.1 mg, 10.31%) as a brown solid. 1H NMR(400MHz,DMSO-d6)δ 14.33(s,1H),12.42(s,1H),8.99(s,1H),8.69(s,1H),8.41(d,J=7.6Hz,1H),8.09( d,J=8.3Hz,1H),7.44(d,J=8.2Hz,2H),7.37(d,J=8.3Hz,2H),7.29(t,J=8.3Hz,1H) ,6.96(d,J=8.0Hz,2H),6.37(s,1H),5.10(d,J=3.5Hz,1H),4.91(p,J=7.5Hz,1H),4 .50(s,2H),4.36(t,J=8.0Hz,1H),4.29(s,1H),3.87-3.64(m,4H),3.60(t,J=4.7Hz, 2H),3.53-3.43(m,1H),3.04-2.97(m,2H),2.96-2.87(m,2H),2.54(s,1H),2.46(s, 3H),2.24(ddd,J=16.3,8.1,4.4Hz,1H),2.02(t,J=10.6Hz,1H),1.92(d,J=12.6Hz, 2H),1.78(ddd,J=12.6,8.0,4.5Hz,1H),1.67(q,J=11.7Hz,2H),1.41(dd,J=30.2,7 .0Hz,3H),0.97(d,J=6.5Hz,3H),0.79(dd,J=11.8,6.7Hz,3H).LCMS(ESI)m / z:[M+H] + =858.2.

[0301] The compounds in Table 15 were prepared according to the following protocols similar to those described above for Compound 15 using the appropriate amine and aldehyde (or ketone), or following procedures similar to those described above for Compound 1 using the appropriate amine and carboxylic acid. [Table 16-1] [Table 16-2] [Table 16-3] [Table 16-4]

[0302] In Table 15, the procedure column lists compounds prepared from the appropriate amine and carboxylic acid as "A" and compounds prepared from the appropriate amine and aldehyde (or ketone) as "B."

[0303] Preparation of 2-(6-{2,6-diazaspiro[3.3]heptan-2-yl}-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-102) [ka] Step 1: Preparation of 7-(benzenesulfonyl)-3,6-dichloropyrrolo[2,3-c]pyridazine (intermediate 2) [ka] To a solution of 7-(benzenesulfonyl)-3-chloropyrrolo[2,3-c]pyridazine (1.5 g, 5.107 mmol, 1 equiv) in THF (30 mL) was added LDA (0.55 g, 5.107 mmol, 1.0 equiv) dropwise over 30 min at −78°C under a dry nitrogen atmosphere. The resulting mixture was stirred at −78°C for 1 h. Next, benzenesulfonyl chloride (0.99 g, 5.618 mmol, 1.1 equiv) was added to the above mixture at −78°C. The resulting mixture was stirred for an additional 1 h at −78°C. The reaction was then quenched with saturated NH4Cl (aq) at −78°C. The aqueous layer was extracted with EtOAc (3 × 100 mL). The combined organic layers were dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography using the following conditions: column, silica gel; mobile phase, MeCN (0.05% FA) in water, gradient 0% to 100% in 40 min; detector, UV 254 nm. This gave intermediate 2 (400 mg, 23.87%) as a yellow solid. LCMS (ESI) m / z [M+H] + =328.

[0304] Step 2: Preparation of tert-butyl 6-{3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl}-2,6-diazaspiro[3.3]heptane-2-carboxylate (Intermediate 3) [ka] A solution of intermediate 2 (200 mg, 0.609 mmol, 1 equiv.), DIEA (1.02 g, 7.917 mmol, 13 equiv.), and tert-butyl 2,6-diazaspiro[3.3]heptane-2-carboxylate (604.16 mg, 3.045 mmol, 5 equiv.) in DMSO (5 mL) was stirred at 80 °C for 4 h under a dry nitrogen atmosphere. The residue was purified by reverse-phase flash chromatography using the following conditions: column, silica gel; mobile phase, MeCN in water, gradient from 0% to 100% in 50 min; detector, UV 254 nm. This gave intermediate 3 (70 mg, 32.83%) as a yellow solid. LCMS (ESI) m / z [M+H] + =350.

[0305] Step 3: Preparation of tert-butyl 6-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]-2,6-diazaspiro[3.3]heptane-2-carboxylate (Intermediate 4) [ka] To a solution of intermediate 3 (70 mg, 0.200 mmol, 1 equiv.) and 2-hydroxyphenylboronic acid (82.80 mg, 0.600 mmol, 3.0 equiv.) in dioxane (2 mL) and HO (0.4 mL), CsCO (195.59 mg, 0.600 mmol, 3.0 equiv.) and XPhos Pd G (33.88 mg, 0.040 mmol, 0.2 equiv.) were added. After stirring at 80 °C under a nitrogen atmosphere for 4 h, the resulting mixture was concentrated under reduced pressure. The residue was purified by preparative TLC / silica gel column chromatography eluting with PE / EA (1:1) to give intermediate 4 (35 mg, 42.93%) as a yellow solid. LCMS (ESI) m / z [M+H] + =408.

[0306] Step 4: Preparation of 2-(6-{2,6-diazaspiro[3.3]heptan-2-yl}-7H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-102) [ka] A solution of intermediate 4 (30 mg, 0.074 mmol, 1 equiv.) and TFA (0.5 mL) in DCM (2 mL) was stirred at room temperature for 1 h. The resulting mixture was concentrated under reduced pressure (the crude product was used directly in the next step without further purification). This gave I-102 (30 mg, crude) as a yellow oil. LCMS (ESI) m / z [M+H] + =308.

[0307] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(2-{6-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]-2,6-diazaspiro[3.3]heptan-2-yl}ethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 226) [ka] To a stirred solution of (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (17.59 mg, 0.033 mmol, 1.00 equiv) and I-102 (10 mg, 0.033 mmol, 1.00 equiv) in DCM (1 mL) and MeOH (1 mL) was added AcOH (0.20 mg, 0.003 mmol, 0.1 equiv) and NaBHCN (10.22 mg, 0.165 mmol, 5.0 equiv) at room temperature. The resulting mixture was stirred for 4 h at room temperature. The resulting mixture was concentrated under reduced pressure. The crude product was purified using the following conditions: column, Kinetex EVO C18 column, 21.2*150 mm, 5 μm; mobile phase, water (10 mmol / L NH4HCO3) and MeOH (57% MeOH, up to 81% in 8 min); detector, UV 254 nm. This gave compound 226 (2.9 mg, 10.71%) as an off-white solid. 1 H NMR(400MHz,DMSO-d6)δ 15.12(s,1H),12.04(s,1H),8.98(s,1H),8.41(d,J=7.6Hz,1H),7.99-7.90(m,2H),7.49-7.33(m,4H),7.28-7.19(m,1H),6.89( t,J=8.2Hz,2H),6.08(s,1H),5.33(d,J=3.7Hz,1H),5.11(d,J=3.6Hz,1H),4.91(p,J=7.4Hz,1H),4.37(t,J=7.9Hz,1H),4.28(s ,1H),4.21(s,4H),4.11(s,2H),3.74-3.62(m,2H),3.61-3.41(m,4H),2.75(s,1H),2.45(s,3H),2.28-2.24(m,1H),2.02(d,J=9 .0Hz,1H),1.78(ddd,J=12.8,8.1,4.8Hz,1H),1.38(d,J=7.0Hz,3H),1.00-0.93(m,3H),0.87-0.77(m,3H).LCMS(ESI)m / z:[M+H] + =832.6.

[0308] The compounds in Table 16 were prepared using procedures similar to those used above for the preparation of compound 226 using the appropriate amine and aldehyde. [Table 17]

[0309] Preparation of 2-[5-(azetidin-3-ylmethoxy)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-103) [ka] Step 1: Preparation of 5-bromo-3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazine [ka] To a stirred solution of 5-bromo-3-chloro-7H-pyrrolo[2,3-c]pyridazine (1000 mg, 4.302 mmol, 1.00 equiv) and SEMCl (1075.76 mg, 6.453 mmol, 1.5 equiv) in DMF (10 mL) was added TEA (870.56 mg, 8.604 mmol, 2 equiv) in portions at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at room temperature for 1 h under a dry nitrogen atmosphere. The resulting mixture was extracted with EtOAc (100 mL × 3). The combined organic layers were washed with brine (50 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by preparative HPLC to give 5-bromo-3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazine (764 mg, 48.96%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =362.00.

[0310] Step 2: Preparation of 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazine [ka] To a stirred solution of 5-bromo-3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazine (630 mg, 1.737 mmol, 1.00 equiv.) and bis(pinacolato)diboron (441.05 mg, 1.737 mmol, 1 equiv.) in 1,4-dioxane (5 mL), AcOK (511.37 mg, 5.211 mmol, 3 equiv.) and Pd(PPh3)4 (401.40 mg, 0.347 mmol, 0.2 equiv.) were added portionwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 100°C for 2 hours under a dry nitrogen atmosphere. The resulting mixture was concentrated under reduced pressure. The crude product was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =410.18.

[0311] Step 3: Preparation of 3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-ol [ka] A stirred solution of 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazine (630 mg, 1.537 mmol, 1.00 equiv) and oxo(sodoperoxy)borane (628.79 mg, 7.685 mmol, 5 equiv) in THF (4 mL) and HO (2 mL) was stirred for 30 min at room temperature. The resulting mixture was extracted with EtOAc (70 mL × 3). The combined organic layers were washed with brine (50 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by preparative HPLC to give 3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-ol (190 mg, 39.95%) as a brown solid. LCMS (ESI) m / z: [M+H] + =300.09.

[0312] Step 4: Preparation of tert-butyl 3-{[(3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl)oxy]methyl}azetidine-1-carboxylate [ka] To a stirred solution of 3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-ol (180 mg, 0.600 mmol, 1.00 equiv.) and tert-butyl 3-(iodomethyl)azetidine-1-carboxylate (267.57 mg, 0.900 mmol, 1.5 equiv.) in DMF (3 mL) was added CsCO (586.81 mg, 1.800 mmol, 3 equiv.) in portions at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 100°C for 2 hours under a dry nitrogen atmosphere. The resulting mixture was concentrated under reduced pressure. The crude product was purified by preparative HPLC to give tert-butyl 3-{[(3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl)oxy]methyl}azetidine-1-carboxylate (93 mg, 33.03%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =489.20.

[0313] Step 5: Preparation of tert-butyl 3-({[3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl]oxy}methyl)azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-{[(3-chloro-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl)oxy]methyl}azetidine-1-carboxylate (82 mg, 0.175 mmol, 1.00 equiv.) and 2-hydroxyphenylboronic acid (72.34 mg, 0.525 mmol, 3 equiv.) in 1,4-dioxane (2 mL) and HO (0.4 mL), XPhos Pd G3 (29.60 mg, 0.035 mmol, 0.2 equiv.) and CsCO3 (170.88 mg, 0.525 mmol, 3 equiv.) were added at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 85°C for 2 hours under a dry nitrogen atmosphere. The resulting mixture was extracted with EtOAc (30 mL × 3). The combined organic layers were washed with brine (20 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give tert-butyl 3-({[3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl]oxy}methyl)azetidine-1-carboxylate (57.2 mg, 62.12%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =527.26.

[0314] Step 6: Preparation of 2-[5-(azetidin-3-ylmethoxy)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-103) [ka] To a stirred solution of tert-butyl 3-({[3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-5-yl]oxy}methyl)azetidine-1-carboxylate (60 mg, 0.114 mmol, 1.00 equiv) in DCM (2.5 mL) was added TFA (0.83 mL) portionwise at room temperature. The resulting mixture was stirred for 1 h at room temperature. The resulting mixture was concentrated under reduced pressure. The crude product (60 mg) was purified by preparative HPLC under the following conditions (Column: SunFire Prep C18 OBD column, 19*150 mm, 5 μm 10 nm; Mobile phase A: water (0.05% FA), Mobile phase B: ACN; Flow rate: 25 mL / min; Gradient: 5% B to 19% B in 4 min; Wavelength: 254 / 220 nm) to give I-103 (6.6 mg, 16.92%) as a yellow solid. 1 H NMR(400MHz,DMSO-d6)δ 13.72(s,1H),12.19(d,J=2.7Hz,1H),8.66(s,2H),8.06(dd,J=8.0,1.6Hz,1H),7.76(d,J=2.6Hz,1H),7.40-7.26(m,1H),7.08-6. 90(m,2H),4.25(d,J=5.2Hz,2H),4.14(p,J=9.1,8.3Hz,2H),3.97(dq,J=12.1,6.5Hz,2H),3.28-3.23(m,1H).LCMS(ESI)m / z:[M+H] + =297.13.

[0315] Preparation of 2-[5-(azetidin-3-yloxy)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-104) [ka] Following the same protocol as for I-103 above, compound I-104 was obtained as a yellow solid. 1H NMR(300MHz,DMSO-d6)δ 13.64(br s,1H),8.59(s,1H),8.28(s,1H,FA),8.12(dd,J=8.0,1.6Hz,1H),7.63(s,1H),7.30(ddd,J=8.4, 7.1,1.6Hz,1H),7.05-6.89(m,2H),5.06(s,1H),4.07(s,2H),3.83(s,2H).LCMS(ESI)m / z:[M+H] + =283.10.

[0316] Preparation of 2-[6-(azetidin-3-yl)-5-cyclopropyl-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-105) [ka] Step 1: Preparation of tert-butyl 3-{3-chloro-5-iodo-7H-pyrrolo[2,3-c]pyridazin-6-yl}azetidine-1-carboxylate [ka] A solution of tert-butyl 3-{3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl}azetidine-1-carboxylate (800 mg, 2.591 mmol, 1.00 equiv) and NIS (582.92 mg, 2.591 mmol, 1 equiv) in DMF (8.00 mL) was stirred under a dry nitrogen atmosphere for 1 h at 0° C. The residue was purified by reverse-phase flash under the following conditions: mobile phase A: water (0.1% FA), mobile phase B: CHCN; flow rate: 60 mL / min; gradient: 0% B to 100% B in 40 min; 254 / 220 nm) to give tert-butyl 3-{3-chloro-5-iodo-7H-pyrrolo[2,3-c]pyridazin-6-yl}azetidine-1-carboxylate (840 mg, 70.86%) as a brown solid. LCMS(ESI)m / z:[M+H] + =435.

[0317] Step 2: 3-(3-chloro-5-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo Preparation of tert-butyl [2,3-c]pyridazin-6-yl)azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-{3-chloro-5-iodo-7H-pyrrolo[2,3-c]pyridazin-6-yl}azetidine-1-carboxylate (840 mg, 1.933 mmol, 1.00 equiv) and TEA (782.22 mg, 7.732 mmol, 4 equiv) in DMF (8.00 mL) was added SEM-Cl (644.39 mg, 3.866 mmol, 2 equiv) dropwise at 0°C under a dry nitrogen atmosphere. The resulting mixture was stirred for 3 h at 0°C. The reaction was quenched with water / ice at 0°C. The resulting mixture was extracted with EtOAc (3 × 10 mL). The combined organic layers were washed with brine (2 × 10 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by preparative TLC (PE / EA 2:1) to give tert-butyl 3-(3-chloro-5-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (1 g, 87.02%) as an orange solid. LCMS (ESI) m / z: [M+H] + =565.

[0318] Step 3: 3-(3-chloro-5-cyclopropyl-7-{[2-(trimethylsilyl)ethoxy]methyl}pyridin Preparation of tert-butyl 2,3-c-pyridazin-6-ylazetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-(3-chloro-5-iodo-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (1 g, 1.770 mmol, 1.00 equiv) and cyclopropyltrifluoro-lambda 4-borane (1.93 g, 17.700 mmol, 10 equiv) in dioxane (10.00 mL) and HO (2.00 mL) was added Pd(dppf)Cl2. CHCl (0.14 g, 0.177 mmol, 0.1 equiv) and KCO (0.73 g, 5.310 mmol, 3 equiv) were added. The resulting mixture was stirred at 80°C overnight under a dry nitrogen atmosphere. HO (20 mL) was added to the resulting mixture, and the mixture was extracted with EtOAc (3 x 20 mL). The combined organic layers were washed with brine (30 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The crude product was purified by preparative TLC (PE / EA 1:1) to afford tert-butyl 3-(3-chloro-5-cyclopropyl-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (295 mg, 30.55%) as an orange oil. LCMS (ESI) m / z: [M+H] + =479.

[0319] Step 4: 3-[5-cyclopropyl-3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl) Preparation of tert-butyl ethoxymethyl}pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-(3-chloro-5-cyclopropyl-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (167 mg, 0.349 mmol, 1.00 equiv.) and 2-hydroxyphenylboronic acid (144.24 mg, 1.047 mmol, 3 equiv.) in dioxane (4.00 mL) and HO (0.80 mL), XPhos Pd G (29.51 mg, 0.035 mmol, 0.1 equiv.) and CsCO (340.72 mg, 1.047 mmol, 3 equiv.) were added portionwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 100°C for 1 hour under a dry nitrogen atmosphere. The residue was filtered. The filtrate was concentrated under reduced pressure and purified by reverse-phase flash under the following conditions: Mobile phase A: water (0.1% FA), Mobile phase B: ACN; Flow rate: 35 mL / min; Gradient: 0% B to 100% B in 40 min; 254 / 220 nm) to give tert-butyl 3-[5-cyclopropyl-3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (160 mg, 81.24%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =537.

[0320] Step 5: Preparation of 2-[6-(azetidin-3-yl)-5-cyclopropyl-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-105) [ka] A solution of tert-butyl 3-[5-cyclopropyl-3-(2-hydroxyphenyl)-7-{[2-(trimethylsilyl)ethoxy]methyl}pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (144 mg, 0.268 mmol, 1.00 equiv) in TFA (2.00 mL) was stirred for 2 hours at 90 °C. The resulting mixture was concentrated under reduced pressure. The crude product was purified by reverse-phase flash chromatography under the following conditions: mobile phase A: water (0.1% FA), mobile phase B: ACN; flow rate: 35 mL / min; gradient: 0% B to 50% B in 30 min; 254 / 220 nm) to afford 2-[6-(azetidin-3-yl)-5-cyclopropyl-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (50 mg, 57.79%) as a yellow solid. LCMS(ESI)m / z:[M+H] + =307.

[0321] Preparation of 2-(5,6,7,8-tetrahydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-106) [ka] Step 1: Preparation of benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-(hydroxymethyl)pyrrolidine-1-carboxylate [ka] To a mixture of benzyl (3S,4S)-3-amino-4-(hydroxymethyl)pyrrolidine-1-carboxylate (2.00 g, 7.990 mmol, 1.00 equiv) and BocO (2.62 g, 11.985 mmol, 1.50 equiv) in THF (30 mL) and HO (10 mL) was added NaHCO (2.01 g, 23.970 mmol, 3.00 equiv). The resulting mixture was stirred for 3 hours at room temperature. The resulting mixture was diluted with water (50 mL), and the resulting mixture was extracted with EtOAc (3 × 100 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-(hydroxymethyl)pyrrolidine-1-carboxylate (3.50 g, crude) as a white solid, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =351.

[0322] Step 2: Preparation of benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-formylpyrrolidine-1-carboxylate [ka] To a mixture of benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-(hydroxymethyl)pyrrolidine-1-carboxylate (3.50 g, 9.988 mmol, 1.00 equiv) in DCM (60 mL) was added DMP (6.35 g, 14.982 mmol, 1.50 equiv). The resulting mixture was stirred for 2 h at room temperature. The resulting mixture was quenched with saturated sodium thiosulfate solution (50 mL) and saturated sodium bicarbonate solution (50 mL), and the resulting mixture was extracted with EtOAc (3×200 mL). The combined organic layers were washed with brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with 0% to 100% EtOAc in PE to give benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-formylpyrrolidine-1-carboxylate (1.10 g, 28.4%) as a white solid. LCMS (ESI) m / z: [M+H] + =349.

[0323] Step 3: Preparation of benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-ethynylpyrrolidine-1-carboxylate [ka] To a mixture of benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-formylpyrrolidine-1-carboxylate (1.10 g, 3.157 mmol, 1.00 equiv) and K2CO3 (1.31 g, 9.471 mmol, 3.00 equiv) in MeOH (30 mL) was added Seyferth-Gilbert reagent (0.91 g, 4.736 mmol, 1.50 equiv) at 0°C. The resulting mixture was stirred at room temperature for 2 hours. The resulting mixture was diluted with water (50 mL) and extracted with EtOAc (100 mL x 3). The combined organic layer was washed with brine (50 mL) and dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure to give benzyl (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-ethynylpyrrolidine-1-carboxylate (1.20 g, crude) as a yellow oil. LCMS (ESI) m / z: [M+H] + =345.

[0324] Step 4: Preparation of benzyl (3S,4S)-3-amino-4-ethynylpyrrolidine-1-carboxylate [ka] To a mixture of (3S,4S)-3-((tert-butoxycarbonyl)amino)-4-ethynylpyrrolidine-1-carboxylate (1.20 g, 3.484 mmol, 1.00 equiv) in DCM (15 mL) was added TFA (5 mL). The resulting mixture was stirred at room temperature for 1 hour. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions (column, C 18 Purification on silica gel (mobile phase, CHCN in water, gradient 0% to 50% in 30 min; detector, UV 220 nm) gave benzyl (3S,4S)-3-amino-4-ethynylpyrrolidine-1-carboxylate (510.0 mg, 53.9%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =245.

[0325] Step 5: Preparation of (4bS,7aS)-3-chloro-4b,7,7a,8-tetrahydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-benzyl carboxylate [ka] To a mixture of benzyl (3S,4S)-3-amino-4-ethynylpyrrolidine-1-carboxylate (510.0 mg, 2.088 mmol, 1.00 equiv) and dichloro-1,2,4,5-tetrazine (945.3 mg, 6.264 mmol, 3.00 equiv) in toluene (20 mL) was added DIEA (1349.11 mg, 10.440 mmol, 5.00 equiv). The resulting mixture was stirred overnight at 100°C. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions (column, C 18 Purification on silica gel (mobile phase, ACN in water, 0% to 50% gradient in 30 min; detector, UV 254 nm) gave benzyl (4bS,7aS)-3-chloro-4b,7,7a,8-tetrahydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (320.0 mg, 41.7%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =.331.

[0326] Step 6: Preparation of benzyl 3-chloro-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate [ka] To a mixture of benzyl (4bS,7aS)-3-chloro-4b,7,7a,8-tetrahydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (320.0 mg, 0.967 mmol, 1.00 equiv.) in DCM (10 mL) was added DMP (820.6 mg, 1.934 mmol, 2.00 equiv.). The resulting mixture was stirred at room temperature for 4 hours. The resulting mixture was filtered, and the filter cake was washed with DCM (3 x 5 mL). The filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions (column, C 18 Purification on silica gel (mobile phase, CAN in water, 0% to 50% gradient in 30 min; detector, UV 254 nm) gave benzyl 3-chloro-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (125.0 mg, 39.3%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =329.

[0327] Step 7: Preparation of benzyl 3-(2-hydroxyphenyl)-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate [ka] To a mixture of benzyl 3-chloro-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (125.0 mg, 0.380 mmol, 1.00 equiv.), 2-hydroxyphenylboronic acid (78.6 mg, 0.570 mmol, 1.5 equiv.), and CsCO (371.6 mg, 1.140 mmol, 3.00 equiv.) in dioxane (5 mL) and HO (1 mL) was added XPhos Pd G (64.3 mg, 0.076 mmol, 0.20 equiv.), and the resulting mixture was stirred at 100°C under a dry nitrogen atmosphere for 1 hour. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions (column, C 18Purification on silica gel (mobile phase, ACN in water, 0% to 50% gradient in 30 min; detector, UV 254 nm) gave benzyl 3-(2-hydroxyphenyl)-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (100.0 mg, 61.2%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =387.

[0328] Step 8: Preparation of 2-(5,6,7,8-tetrahydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-106) [ka] To a mixture of benzyl 3-(2-hydroxyphenyl)-7,8-dihydropyrrolo[3',4':4,5]pyrrolo[2,3-c]pyridazine-6(5H)-carboxylate (100.0 mg, 0.259 mmol, 1.00 equiv.) in DCM (5 mL) was added BBr3 (648.3 mg, 2.590 mmol, 10.00 equiv.) at 0°C. The resulting mixture was stirred at 0°C for 1 h. The resulting mixture was quenched with MeOH (1 mL) and then concentrated under reduced pressure. The residue was purified by preparative HPLC under the following conditions: column: Gemini-NX C packed 18 Purification with AXAI, 21.2*150 mm 5 μm; Mobile phase A: water (0.05% FA), Mobile phase B: MeOH; Flow rate: 25 mL / min; Gradient: 22% B to 68% B, 68% B in 7 min; Wavelength: 220 / 254 nm gave I-106 (35.0 mg, 53.6%) as a yellow solid. 1 H-NMR(400MHz,DMSO-d6)δ 14.2(s,1H),8.51(s,1H),8.18(s,1H),8.04(dd,J=8.0,1.6Hz,1H),7.29(ddd,J=8.4 ,7.1,1.6Hz,1H),7.00-6.90(m,2H),4.21(s,2H),4.14(s,2H).LCMS(ESI)m / z:[M+H] + =253.10.

[0329] Preparation of 1-{3-[5-bromo-3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidin-1-yl}ethanone (I-107) [ka] Step 1: 3-[5-bromo-3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazine Preparation of tert-butyl azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (100 mg, 0.273 mmol, 1.00 equiv) in THF (4.00 mL) was added NBS (38.86 mg, 0.218 mmol, 0.8 equiv) in portions at room temperature. The resulting mixture was stirred at room temperature for 30 minutes under a dry nitrogen atmosphere. The resulting mixture was diluted with water (30 mL) and extracted with EtOAc (3 × 20 mL). The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by preparative TLC (PE / EA 1:1) to give tert-butyl 3-[5-bromo-3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (75 mg, 55.54%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =445.

[0330] Step 2: Preparation of 2-[6-(azetidin-3-yl)-5-bromo-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-107) [ka] A solution of tert-butyl 3-[5-bromo-3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (70 mg, 0.157 mmol, 1.00 equiv.) and TFA (1.00 mL) in DCM (3.00 mL) was stirred at room temperature under a dry nitrogen atmosphere for 1 hour. The resulting mixture was concentrated under reduced pressure to give crude 2-[6-(azetidin-3-yl)-5-bromo-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (70 mg), which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =345.

[0331] The following intermediates in Table 17 were prepared in a manner similar to that described in the preparation of intermediate I-107. [Table 18]

[0332] Preparation of 2-[6-(azetidin-3-yl)-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-110) [ka] Step 1: Preparation of tert-butyl 3-[3-chloro-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate [ka] To a stirred mixture of tert-butyl 3-{3-chloro-7H-pyrrolo[2,3-c]pyridazin-6-yl}azetidine-1-carboxylate (175 mg, 0.567 mmol, 1.00 equiv) and (bromodifluoromethyl)trimethylsilane (460.44 mg, 2.268 mmol, 4 equiv) in CHCN (5 mL) was added t-BuOK (254.39 mg, 2.268 mmol, 4 equiv) portionwise at room temperature. The resulting mixture was stirred overnight at room temperature. The desired product could be detected by LCMS. The reaction was quenched at room temperature by adding water (50 mL). The resulting mixture was extracted with EtOAc (3 × 50 mL). The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give tert-butyl 3-[3-chloro-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (130 mg, 58.82%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =359.

[0333] Step 2: Preparation of tert-butyl 3-[7-(difluoromethyl)-3-(2-hydroxyphenyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate [ka] To a solution of tert-butyl 3-[3-chloro-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (130 mg, 0.362 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (149.94 mg, 1.086 mmol, 3 equiv) in dioxane (2.5 mL) and HO (0.5 mL) was added CsCO (354.18 mg, 1.086 mmol, 3 equiv) and XPhos Pd G (61.34 mg, 0.072 mmol, 0.2 equiv). After stirring at 80 °C under a nitrogen atmosphere for 3 h, the resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give tert-butyl 3-[7-(difluoromethyl)-3-(2-hydroxyphenyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (100 mg, 59.65%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =417.

[0334] Step 3: Preparation of 2-[6-(azetidin-3-yl)-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-110) [ka] To a stirred mixture of tert-butyl 3-[7-(difluoromethyl)-3-(2-hydroxyphenyl)pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylate (100 mg, 0.240 mmol, 1 equiv.) in DCM (3 mL) was added TFA (1 mL) dropwise at room temperature. The resulting mixture was stirred at room temperature for 1 hour. The desired product could be detected by LCMS. The resulting mixture was concentrated under reduced pressure to give 2-[6-(azetidin-3-yl)-7-(difluoromethyl)pyrrolo[2,3-c]pyridazin-3-yl]phenol (110 mg, 95.80%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =317.

[0335] Preparation of (2S,4R)-1-[(2R)-2-(3-{1-[(6R*)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carbonyl]piperidin-4-yl}-1,2-oxazol-5-yl)-3-methylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (I-111) [ka] Step 1: Preparation of methyl 1-[(tert-butoxycarbonyl)amino]-4-oxocyclohexane-1-carboxylate [ka] To a stirred solution of methyl 1-amino-4-oxocyclohexane-1-carboxylate hydrochloride (5.00 g, 24.079 mmol, 1.00 equiv) in THF (50.00 mL) was added (Boc)2O (15.77 g, 72.237 mmol, 3.00 equiv) and TEA (12.18 g, 120.395 mmol, 5.00 equiv) at 0 °C. The resulting mixture was stirred for 2 h at room temperature. The reaction was quenched with water at 0 °C. The resulting mixture was extracted with DCM (2 × 100 mL). The combined organic layers were concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN in water, 0 to 100% gradient in 30 min; detector, UV 220 / 200 nm. This gave methyl 1-[(tert-butoxycarbonyl)amino]-4-oxocyclohexane-1-carboxylate (3.8 g, 58.17%) as a white solid. LCMS (ESI) m / z: [M+H] + =272.

[0336] Step 2: Preparation of methyl 6-[(tert-butoxycarbonyl)amino]-3-chloro-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate [ka] To a stirred solution of methyl 1-[(tert-butoxycarbonyl)amino]-4-oxocyclohexane-1-carboxylate (3.80 g, 14.743 mmol, 1.00 equiv) in pyridine (15.00 mL) was added 4-bromo-6-chloropyridazin-3-amine (3.07 g, 14.743 mmol, 1.00 equiv) and Pd(PPh3)4 (1.70 g, 1.474 mmol, 0.10 equiv) at room temperature. The resulting mixture was stirred at 150 °C for 1 hour under a nitrogen and microwave atmosphere. The mixture was allowed to cool to room temperature. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 220 / 200 nm. This gave methyl 6-[(tert-butoxycarbonyl)amino]-3-chloro-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (469 mg, 8.35%) as a brown oil. LCMS (ESI) m / z: [M+H] + =381.

[0337] Step 3: Preparation of methyl 6-[(tert-butoxycarbonyl)amino]-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate [ka] To a stirred solution of methyl 6-[(tert-butoxycarbonyl)amino]-3-chloro-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (469.00 mg, 1.205 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (498.73 mg, 3.615 mmol, 3.00 equiv) in 1,4-dioxane (8.00 mL) and HO (2.00 mL) was added XPhos Pd G (102.02 mg, 0.121 mmol, 0.10 equiv) and CsCO (1.18 g, 3.622 mmol, 3.00 equiv) at room temperature. The resulting mixture was stirred at 80 °C for 2 h under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 254 / 220 nm. This afforded methyl 6-[(tert-butoxycarbonyl)amino]-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (218 mg, 41.25%) as a brown solid. LCMS (ESI) m / z: [M+H] + =439.

[0338] Step 4: Preparation of methyl 6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate [ka] To a stirred solution of methyl 6-[(tert-butoxycarbonyl)amino]-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (218.00 mg, 0.497 mmol, 1.00 equiv) in DCM (10.00 mL) was added TFA (5.00 mL) at room temperature. The resulting mixture was stirred for 1 hour at room temperature. The resulting mixture was concentrated under reduced pressure. The resulting mixture was washed with DCM (2 x 100 mL). The combined aqueous layers were concentrated under reduced pressure. This afforded methyl 6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (103 mg, 61.23%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =339.

[0339] Step 5: Preparation of methyl (6S)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate [ka] 6-Amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylic acid (103.00 mg) was purified by chiral preparative HPLC using the following conditions: column, CHIRAL ART Amylose-SA, 2*25 cm, 5 μm; mobile phase, Hex:MTBE = 1:1 (0.5% 2M NH3-MeOH) and EtOH (hold 30% EtOH in 25 min); detector, UV 254 / 220 nm. This gave two isomers (34 mg and 30 mg) as yellow solids. LCMS (ESI) m / z: [M+H] + =339.

[0340] Step 6: Preparation of (6S)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylic acid (I-111) [ka] To a stirred solution of methyl (6S)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylate (15.00 mg, 0.100 mmol, 1.00 equiv) in THF (1.50 mL) and HO (1.50 mL) was added LiOH . HO (12.65 mg, 0.300 mmol, 3.00 equiv) was added at room temperature. The resulting mixture was stirred for 2 h at room temperature. The residue was acidified to pH 6 with aqueous HCl (1 mol / L). The resulting mixture was extracted with EA (2 × 100 mL). The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This gave (6S)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylic acid (14 mg, crude) as a yellow oil. LCMS (ESI) m / z: [M+H] + =325. [ka]

[0341] Following the same protocol, (6R)-6-amino-3-(2-hydroxyphenyl)-5H,7H,8H,9H-pyridazino[3,4-b]indole-6-carboxylic acid (14 mg, crude) was obtained as a yellow oil. LCMS (ESI) m / z: [M+H] + =325. .

[0342] Preparation of 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoic acid (I-113) [ka] Step 1: Preparation of tert-butyl 4-(2-methoxypyrimidin-5-yl)piperidine-1-carboxylate [ka] (5-Bromo-2-methoxypyrimidine (3.00 g, 15.872 mmol, 1.00 equiv), dtbpy (0.43 g, 1.587 mmol, 0.10 equiv), Mn (1.74 g, 31.744 mmol, 2.00 equiv), KI (2.63 g, 15.872 mmol, 1.00 equiv), pyridine (1.38 g, 17.459 mmol, 1.10 equiv), and NiBr in DMA (10 mL). to a solution of 2-diglyme (0.56 g, 1.587 mmol, 0.10 equiv). The mixture was stirred at 80 °C under a nitrogen atmosphere for 15 hours. The desired product could be detected by LCMS. The reaction mixture was filtered through a short pad of Celite and concentrated in vacuo. The residue was purified by reverse-phase flash chromatography under the following conditions (column: C18 silica gel; mobile phase: MeCN (0.1% FA) in water, gradient from 10% to 100% in 30 min; detector: UV 254 nm) to give tert-butyl 4-(2-methoxypyrimidin-5-yl)piperidine-1-carboxylate (1.76 g, 37.80%) as a brown oil. LCMS (ESI) m / z [M+H] + =294.

[0343] Step 2: Preparation of 2-methoxy-5-(piperidin-4-yl)pyrimidine [ka] To a solution of tert-butyl 4-(2-methoxypyrimidin-5-yl)piperidine-1-carboxylate (1.70 g, 5.795 mmol, 1.00 equiv) in DCM (10.0 mL) was added TFA (3.0 mL). After stirring at room temperature for 1 hour, the desired product could be detected by LCMS. The resulting mixture was concentrated under reduced pressure to give 2-methoxy-5-(piperidin-4-yl)pyrimidine (860 mg, 76.80%) as a brown oil. LCMS (ESI) m / z [M+H] + =194.

[0344] Step 3: Preparation of methyl 2-(3-(4-(2-methoxypyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate [ka] A mixture of 2-methoxy-5-(piperidin-4-yl)pyrimidine (860.0 mg, 4.450 mmol, 1 equiv.), methyl 3-methyl-2-{3-[(1,1,2,2,3,3,4,4,4-nonafluorobutanesulfonyl)oxy]-1,2-oxazol-5-yl}butanoate (3.21 g, 6.675 mmol, 1.50 equiv.), and DIEA (1.73 g, 13.350 mmol, 3.00 equiv.) in DMF (5 mL) was stirred for 3 hours at 130° C. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions (column: C18 silica gel; mobile phase: MeCN (0.1% FA) in water, 10% to 100% gradient in 30 min; detector: UV 254 nm) to give methyl 2-(3-(4-(2-methoxypyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (320.0 mg, 19.20%) as a brown solid. LCMS (ESI) m / z [M+H] + =375.

[0345] Step 4: Preparation of methyl 2-(3-(4-(2-chloropyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate [ka] A mixture of methyl 2-(3-(4-(2-methoxypyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (300.0 mg, 0.801 mmol, 1.00 equiv) and POCl3 (1.23 g, 8.010 mmol, 10.00 equiv) in DMF (2 mL) was stirred at 100°C for 5 hours. The desired product could be detected by LCMS. The reaction was quenched with water at 0°C. The residue was purified by reverse-phase flash chromatography under the following conditions (column: C18 silica gel; mobile phase: MeCN (0.1% FA) in water, gradient from 10% to 100% in 10 min; detector: UV 254 nm) to give methyl 2-(3-(4-(2-chloropyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (60.0 mg, 19.77%) as a brown solid. LCMS (ESI) m / z [M+H] + =379.

[0346] Step 5: Preparation of methyl 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate [ka] A mixture of methyl 2-(3-(4-(2-chloropyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (60.0 mg, 0.158 mmol, 1.00 equiv), 2-[5-(piperidin-4-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (46.6 mg, 0.158 mmol, 1.00 equiv), and DIEA (61.4 mg, 0.474 mmol, 3.00 equiv) in DMF (2 mL) was stirred at 120° C. for 10 hours. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions (column: C18 silica gel; mobile phase: MeCN (0.1% FA) in water, 10% to 100% gradient in 40 min; detector: UV 254 nm) to give methyl 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (70 mg, 69.41%) as an off-white solid. LCMS (ESI) m / z [M+H] + =637.

[0347] Step 6: Preparation of 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoic acid (I-113) [ka] A mixture of methyl 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoate (62.0 mg, 0.097 mmol, 1.00 equiv) and LiOH (11.6 mg, 0.485 mmol, 5.00 equiv) in MeOH (1.5 mL) and HO (0.3 mL) was stirred at room temperature for 15 h. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions (column: silica gel; mobile phase: MeCN in water, gradient from 10% to 100% in 30 min; detector: UV 254 nm) to give 2-(3-(4-(2-(4-(3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidin-1-yl)pyrimidin-5-yl)piperidin-1-yl)isoxazol-5-yl)-3-methylbutanoic acid (42 mg, 69.27%) as a yellow solid. LCMS (ESI) m / z [M+H] + =623.

[0348] Preparation of 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoic acid (I-114) [ka] Step 1: (E)-N-[(2-chloropyrimidin-5-yl)methylidene]hydroxylamine [ka] 2-Chloropyrimidine-5-carbaldehyde (5 g, 35.078 mmol, 1 equiv.) and NHOH in EtOH (250 mL) .To a stirred solution of HCl (4.93 g, 70.945 mmol, 2.02 equiv) was added NaOAc (14.48 g, 176.512 mmol, 5.03 equiv) dropwise at room temperature. The resulting mixture was stirred for 2 h at room temperature. The solvent was removed under reduced pressure. The residue was dissolved in EtOAc (500 mL). The organic layer was washed with brine (500 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure to give (E)-N-[(2-chloropyrimidin-5-yl)methylidene]hydroxylamine (4.6 g, crude) as a pale yellow solid, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =158.

[0349] Step 2: Preparation of (Z)-2-chloro-N-hydroxypyrimidine-5-carbonimidoyl chloride [ka] A solution of (E)-N-[(2-chloropyrimidin-5-yl)methylidene]hydroxylamine (4.6 g, 29.195 mmol, 1 equiv.) and NCS (4.4 g, 32.951 mmol, 1.13 equiv.) in DMF (150 mL) was stirred for 2 h at room temperature. The residue was diluted with EtOAc (500 mL). The resulting mixture was washed with water (3 × 300 mL), brine (1 × 300 mL), and the organic phase was dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give (Z)-2-chloro-N-hydroxypyrimidine-5-carbonimidoyl chloride (4.8 g, crude) as a yellow solid. LCMS (ESI) m / z: [M+H] + =192.

[0350] Step 3: Preparation of methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]acetate [ka] A solution of (Z)-2-chloro-N-hydroxypyrimidine-5-carbonimidoyl chloride (4.8 g, 25.00 mmol, 1 equiv) in EtOAc (80 mL) was treated with NaHCO (3 g, 35.712 mmol, 1.43 equiv) under a dry nitrogen atmosphere for 30 min at 0 °C, followed by the addition of methyl but-3-ynoate (2.02 g, 20.591 mmol, 0.82 equiv) in portions at 0 °C. The resulting mixture was stirred for an additional 12 h at room temperature. The resulting mixture was diluted with water (150 mL) and extracted with EtOAc (2 × 400 mL). The combined organic layers were washed with brine (1 × 400 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]acetate (2.5 g, 38.64%) as a pale yellow solid. LCMS (ESI) m / z: [M+H] + =254.

[0351] Step 4: Preparation of [3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetic acid [ka] A solution of methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]acetate (3 g, 11.828 mmol, 1 equiv.) and NaOMe (1.92 g, 35.484 mmol, 3.00 equiv.) in MeOH (50 mL) was stirred at room temperature under a dry nitrogen atmosphere for 1 h. The mixture was acidified to pH 6 with HCl (aq.). The residue was dissolved in EtOAc (300 mL). The resulting mixture was washed with water (2 × 300 mL). The combined organic layers were washed with brine (1 × 300 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure to give [3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetic acid (2.5 g, crude) as a pale yellow solid, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =236.

[0352] Step 5: Preparation of methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetate [ka] A solution of [3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetic acid (2.4 g, 10.204 mmol, 1 equiv.) and 1-[(trimethylsilyl)methylidene]-1 lambda 5-diazen-1-ium-2-id-1-ylidene (2.33 g, 20.408 mmol, 2 equiv.) in DCM (20 mL) and MeOH (5 mL) was stirred at room temperature for 30 minutes. The resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetate (1.2 g, 45.77%) as a white solid. LCMS (ESI) m / z: [M+H] + =250.

[0353] Step 6: Preparation of methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate [ka] A solution of methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]acetate (2.5 g, 10.031 mmol, 1 equiv) in THF (20 mL) was treated with t-BuOK (1.2 g, 10.694 mmol, 1.07 equiv) under a dry nitrogen atmosphere for 30 min at 0 °C, and then 2-iodopropane (1.5 g, 8.824 mmol, 0.88 equiv) was added dropwise at 0 °C. The resulting mixture was stirred for an additional 12 h at room temperature. The mixture was acidified to pH 6 with HCl (aq). The resulting mixture was extracted with EtOAc (2 × 200 mL). The combined organic layers were washed with brine (2 × 200 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (3:1) to give methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate (310 mg, 10.08%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =292.

[0354] Step 7: Preparation of methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate [ka] A solution of methyl 2-[3-(2-methoxypyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate (200 mg, 0.687 mmol, 1 equiv.) and POCl3 (1.9 mL, 20.61 mmol, 30 equiv.) in DMF (1.5 mL) was stirred at room temperature under a dry nitrogen atmosphere for 3 h. The residue was dissolved in EtOAc (100 mL). The resulting mixture was washed with 2 × 100 mL of brine and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure to give methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate (160 mg, crude) as a brown oil, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] +=296.

[0355] Step 8: Preparation of methyl 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoate [ka] To a stirred mixture of methyl 2-[3-(2-chloropyrimidin-5-yl)-1,2-oxazol-5-yl]-3-methylbutanoate (270 mg, 0.913 mmol, 1 equiv.) and 2-{4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-12-yl}phenol (243.14 mg, 0.913 mmol, 1 equiv.) in DMF (5 mL) was added dropwise DIEA (354.02 mg, 2.739 mmol, 3 equiv.) at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred for an additional 2 h at 60 °C. The resulting mixture was extracted with EtOAc (2 × 50 mL). The combined organic layers were washed with brine (2 × 50 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0% to 100% gradient in 35 min; detector, UV 254 / 220 nm. This gave methyl 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoate (82 mg, 17.09%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =526.

[0356] Step 9: Preparation of 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoic acid (I-114) [ka] To a stirred solution of methyl 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}] trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoate (82 mg, 0.156 mmol, 1 equiv) in MeOH (3.00 mL) and HO (3.00 mL) was added LiOH . HO (65.47 mg, 1.560 mmol, 10 equiv) was added at room temperature. The resulting mixture was stirred for 2 h at room temperature. The residue was acidified to pH 6 with HCl (1 M, aq.). The resulting mixture was extracted with CHCl / 2-propanol (3:1) (2 × 50 mL). The combined organic layers were washed with water (2 × 50 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This gave 2-(3-{2-[12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-4-yl]pyrimidin-5-yl}-1,2-oxazol-5-yl)-3-methylbutanoic acid (80 mg, crude) as a yellow oil, which was used directly in the next step without further purification. LCMS(ESI)m / z:[M+H] + =512.

[0357] Preparation of 12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboximidamide (I-115) [ka] Step 1: Preparation of tert-butyl 12-chloro-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate [ka] A solution of tert-butyl 4-oxopiperidine-1-carboxylate (7 g, 35.132 mmol, 1 equiv.), 4-bromo-6-chloropyridazin-3-amine (8.79 g, 42.158 mmol, 1.2 equiv.), Pd(OAc) (1.58 g, 7.026 mmol, 0.2 equiv.), and 1,4-diazabicyclo[2.2.2]octane (11.82 g, 105.396 mmol, 3 equiv.) in DMF (100 mL) was stirred at 120 °C for 8 hours under a dry nitrogen atmosphere. The resulting mixture was filtered, and the filter cake was washed with CHCN (3 × 60 mL). The filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, CHCN in water, gradient 0% to 100% in 40 min; detector, UV 254 nm to give tert-butyl 12-chloro-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate (419 mg, 3.86%) as a brown solid. LCMS (ESI) m / z: [M+H] + =309.

[0358] Step 2: Preparation of tert-butyl 12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate [ka] A solution of tert-butyl 12-chloro-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate (210 mg, 0.680 mmol, 1 equiv.), 2-hydroxyphenylboronic acid (140.71 mg, 1.020 mmol, 1.5 equiv.), XPhos Pd G3 (115.14 mg, 0.136 mmol, 0.2 equiv.), and Cs2CO3 (664.79 mg, 2.040 mmol, 3 equiv.) in 1,4-dioxane (5 mL) and HO (1 mL) was stirred at 100 °C for 2 h under a dry nitrogen atmosphere. The resulting mixture was filtered, and the filter cake was washed with EA (3 × 10 mL). The filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, CHCN in water, gradient 0% to 100% in 40 min; detector, UV 254 nm to give tert-butyl 12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate (157 mg, 62.91%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =367.

[0359] Step 3: Preparation of 2-{4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-12-yl}phenol [ka] A solution of tert-butyl 12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboxylate (157 mg, 0.428 mmol, 1 equiv.) and TFA (2 mL) in DCM (2 mL) was stirred for 1 h at room temperature. The resulting mixture was concentrated in vacuo. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, CHCN in water, gradient from 0% to 100% in 40 min; detector, UV 254 nm to give 2-{4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-12-yl}phenol (97 mg, 84.88%) as a brown solid. LCMS(ESI)m / z:[M+H] + =267.

[0360] Step 4: Preparation of 12-(2-hydroxyphenyl)-4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraene-4-carboximidamide (I-115) [ka] A solution of 2-{4,8,10,11-tetraazatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7),10,12-tetraen-12-yl}phenol (35 mg, 0.131 mmol, 1 equiv.), 1,2,4-triazole-1-carboximidamide (17.52 mg, 0.157 mmol, 1.2 equiv.), and DIEA (84.93 mg, 0.655 mmol, 5 equiv.) in DMF (1 mL) was stirred at room temperature under a dry nitrogen atmosphere for 12 h. The crude product was purified by preparative HPLC under the following conditions (Column: SunFire Prep C18 OBD column, 19*150 mm, 5 μm; Mobile phase A: water (0.05% FA), Mobile phase B: CH3CN; Flow rate: 25 mL / min; Gradient: 2% B to 18% B to 18% B in 7 min) to give I-115 (22.3 mg, 55.09%) as a yellow solid. 1H NMR(300MHz,DMSO-d6)δ 13.95(s,1H),8.58-8.26(m,5H),8.00(d,J=7.8Hz,1H),7.36-7.25(m,1H),7.03-6.92(m,2H),4.74(s,2H),3.86(t,J= 3.2Hz,2H),3.05(s,J=4.7Hz,2H).LCMS(ESI)m / z:[M+H] + =309.10.

[0361] Preparation of 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoic acid (I-116) [ka] Step 1: Preparation of ethyl 4-(2-methoxypyrimidin-5-yl)cyclohex-3-ene-1-carboxylate [ka] To a stirred solution of 5-bromo-2-methoxypyrimidine (6.00 g, 31.746 mmol, 1.00 equiv) and ethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)cyclohex-3-ene-1-carboxylate (8.89 g, 31.746 mmol, 1.00 equiv) in 1,4-dioxane (40.00 mL) and HO (10.00 mL), Pd(dppf)Cl-CHCl (2.60 g, 3.175 mmol, 0.10 equiv) and KCO (13.14 g, 95.238 mmol, 3.00 equiv) were added at room temperature. The resulting mixture was stirred at 80 °C for 2 hours under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1 / 2) to give ethyl 4-(2-methoxypyrimidin-5-yl)cyclohex-3-ene-1-carboxylate (7.20 g, 86.54%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =263.

[0362] Step 2: Preparation of ethyl 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carboxylate [ka] To a stirred solution of ethyl 4-(2-methoxypyrimidin-5-yl)cyclohex-3-ene-1-carboxylate (7.20 g, 27.481 mmol, 1.00 equiv) in THF (50.00 mL) was added Pd(OH)2 / C (3.85 g, 27.481 mmol, 1.00 equiv) at room temperature. The resulting mixture was stirred under a hydrogen atmosphere at room temperature for 16 hours. The resulting mixture was filtered, and the filter cake was washed with THF. The filtrate was concentrated under reduced pressure. This afforded ethyl 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carboxylate (6.00 g, 82.76%) as a gray oil. LCMS (ESI) m / z: [M+H] + =265.

[0363] Step 3: Preparation of [4-(2-methoxypyrimidin-5-yl)cyclohexyl]methanol [ka] To a stirred solution of ethyl 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carboxylate (6.00 g, 22.727 mmol, 1.00 equiv) in THF (50.00 mL) was added LiAlH (9.09 mL, 22.727 mmol, 1.00 equiv, 2.50 mol / L) at 0 °C under a dry nitrogen atmosphere. The resulting mixture was stirred at 0 °C for 1 h under a dry nitrogen atmosphere. The reaction was quenched with water at 0 °C. The resulting mixture was extracted with DCM (2 × 100 mL). The combined organic layers were washed with saturated brine (1 × 100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This afforded [4-(2-methoxypyrimidin-5-yl)cyclohexyl]methanol (2.70 g, 53.46%) as a yellow oil. LCMS(ESI)m / z:[M+H] + =223.

[0364] Step 4: Preparation of 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carbaldehyde [ka] To a stirred solution of (COCl) (4.63 g, 36.486 mmol, 3.00 equiv) in DCM (30.00 mL) was added DMSO (3.79 g, 48.649 mmol, 4.00 equiv) at −70°C under a dry nitrogen atmosphere. The resulting mixture was stirred at −78°C for 30 minutes under a dry nitrogen atmosphere. To the above mixture was added [4-(2-methoxypyrimidin-5-yl)cyclohexyl]methanol (2.70 g, 12.162 mmol, 1.00 equiv) at −70°C under a dry nitrogen atmosphere. The resulting mixture was stirred at −70°C for an additional 30 minutes under a dry nitrogen atmosphere. To the above mixture was added EtN (6.14 g, 60.811 mmol, 5.00 equiv) at −70°C under a dry nitrogen atmosphere. The resulting mixture was stirred at room temperature for an additional 1 hour. The reaction was quenched at 0° C. by adding water (100 mL). The resulting mixture was extracted with DCM (2×100 mL). The combined organic layers were washed with brine (2×100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This gave 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carbaldehyde (2.40 g, 89.55%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =221.

[0365] Step 5: Preparation of (E)-N-{[4-(2-methoxypyrimidin-5-yl)cyclohexyl]methylidene}hydroxylamine [ka] To a stirred solution of hydroxylamine hydrochloride (2.46 g, 35.412 mmol, 3.00 equiv) in MeOH (8.00 mL) and HO (24.00 mL) was added NaCO (3.75 g, 35.412 mmol, 3.00 equiv) at 0 °C. To the above mixture was added 4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carbaldehyde (2.40 g, 11.804 mmol, 1.00 equiv) at 0 °C. The resulting mixture was stirred for an additional 1 h at room temperature. The reaction was quenched with water at 0 °C. The resulting mixture was extracted with EA (2 × 200 mL). The combined organic layers were washed with saturated brine (1 × 200 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This gave (E)-N-{[4-(2-methoxypyrimidin-5-yl)cyclohexyl]methylidene}hydroxylamine (2.80 g, crude) as a yellow oil. LCMS (ESI) m / z: [M+H] + =236.

[0366] Step 6: Preparation of (Z)-N-hydroxy-4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carbonimidoyl chloride [ka] To a stirred solution of (E)-N-{[4-(2-methoxypyrimidin-5-yl)cyclohexyl]methylidene}hydroxylamine (2.80 g, 11.900 mmol, 1.00 equiv) in EA (30.00 mL) was added NCS (1.91 g, 14.280 mmol, 1.20 equiv) at 0° C. The resulting mixture was stirred at room temperature for 1 h. The resulting mixture was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =270.

[0367] Step 7: Preparation of methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}acetate [ka] To a stirred solution of methyl but-3-ynoate (4.66 g, 47.456 mmol, 4.00 equiv) in EA (30.00 mL) was added NaHCO (2.99 g, 35.592 mmol, 3.00 equiv) at 0 °C. To the above mixture was added (Z)-N-hydroxy-4-(2-methoxypyrimidin-5-yl)cyclohexane-1-carbonimidoyl chloride (3.20 g, 11.864 mmol, 1.00 equiv) at 0 °C. The resulting mixture was stirred for an additional 16 h at room temperature. The reaction was quenched with water at 0 °C. The resulting mixture was extracted with EA (2 × 200 mL). The combined organic layers were concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 254 / 220 nm. This gave the intermediate methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}acetate (2.70 g, 68.68%) as a brown oil. LCMS (ESI) m / z: [M+H] + =332.

[0368] Step 8: Preparation of methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate [ka] To a stirred solution of methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}acetate (2.70 g, 8.132 mmol, 1.00 equiv) in THF (30.00 mL) was added t-BuOK (24.40 mL, 24.398 mmol, 3.00 equiv, 1.0 mol / L) at 0°C under a dry nitrogen atmosphere. The resulting mixture was stirred at 0°C for 30 minutes under a dry nitrogen atmosphere. To the above mixture was added 2-iodopropane (4.15 g, 24.398 mmol, 3.00 equiv) at 0°C. The resulting mixture was stirred for an additional 2 hours at room temperature. The reaction was quenched with water (100 mL) at 0°C. The residue was acidified to pH 3 with HCl (1 mol / L). The resulting mixture was extracted with DCM (2 × 200 mL). The combined organic layers were concentrated under reduced pressure. The residue was dissolved in DCM (16.00 mL) and MeOH (4.00 mL). To the above mixture, CH2N2 (1.02 g, 24.396 mmol, 3.00 equiv) was added at 0 °C. The resulting mixture was stirred at room temperature for an additional 1 h. The reaction was quenched with water at 0 °C. The resulting mixture was extracted with DCM (2 × 200 mL). The combined organic layers were concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 254 / 220 nm. This gave methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (795 mg, 38.26%) as a brown oil. LCMS (ESI) m / z: [M+H] + =374.

[0369] Step 9: Preparation of methyl 2-{3-[4-(2-chloropyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate [ka] To a stirred solution of methyl 2-{3-[4-(2-methoxypyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (795.00 mg, 2.129 mmol, 1.00 equiv) in DMF (5.00 mL) was added POCl3 (979.15 mg, 6.387 mmol, 3.00 equiv) at room temperature. The resulting mixture was stirred at 80 °C for 16 h. The mixture was allowed to cool to room temperature. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 254 / 220 nm. This gave methyl 2-{3-[4-(2-chloropyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (154 mg, 19.14%) as a brown oil. LCMS (ESI) m / z: [M+H] + =378.

[0370] Step 10: Preparation of methyl 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate [ka] To a stirred solution of methyl 2-{3-[4-(2-chloropyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (80.00 mg, 0.212 mmol, 1.00 equiv) in DMSO (2.00 mL) was added 2-[5-(piperidin-4-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (62.32 mg, 0.212 mmol, 1.00 equiv) and DIEA (136.82 mg, 1.060 mmol, 5.00 equiv) at room temperature. The resulting mixture was stirred at 100° C. for 1 hour. The mixture was allowed to cool to room temperature. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, 0 to 100% gradient in 30 min; detector, UV 254 / 220 nm. This gave methyl 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (61 mg, 45.32%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =636.

[0371] Step 11: Preparation of 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoic acid [ka] To a stirred solution of methyl 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoate (61.00 mg, 0.093 mmol, 1.00 equiv) in MeOH (2.00 mL) and HO (2.00 mL) was added LiOH .HO (11.68 mg, 0.279 mmol, 3.00 equiv) was added at room temperature. The resulting mixture was stirred for 1 hour at room temperature. The residue was acidified to pH 3 with aqueous HCl (1 mol / L). The resulting mixture was concentrated under reduced pressure. This gave 2-{3-[4-(2-{4-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}pyrimidin-5-yl)cyclohexyl]-1,2-oxazol-5-yl}-3-methylbutanoic acid (60 mg, crude) as a yellow solid. LCMS (ESI) m / z: [M+H] + =622.

[0372] Preparation of 5-(azetidin-3-yl)-3-(2-hydroxyphenyl)-7H-imidazo[4,5-c]pyridazin-6-one (I-117) [ka] Step 1: Preparation of tert-butyl 3-[(3-amino-6-chloropyridazin-4-yl)amino]azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-aminoazetidine-1-carboxylate (1.65 g, 9.596 mmol, 2 equiv.) and DIEA (6.20 g, 47.980 mmol, 10 equiv.) in DMSO (16 mL) was added 4-bromo-6-chloropyridazin-3-amine (1 g, 4.798 mmol, 1.00 equiv.). The resulting mixture was stirred at 120°C for 2 hours. The reaction mixture was poured into 100 mL of water, and the precipitated solid was collected by filtration, washed with HO, and dried under vacuum to give tert-butyl 3-[(3-amino-6-chloropyridazin-4-yl)amino]azetidine-1-carboxylate (910 mg, 62.58%) as a white solid, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =300.

[0373] Step 2: Preparation of tert-butyl 3-{3-chloro-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl}azetidine-1-carboxylate [ka] To a stirred solution of tert-butyl 3-[(3-amino-6-chloropyridazin-4-yl)amino]azetidine-1-carboxylate (500 mg, 1.668 mmol, 1.00 equiv) and TEA (675.14 mg, 6.672 mmol, 4 equiv) in THF (5 mL) was added triphosgene (989.95 mg, 3.336 mmol, 2 equiv). The resulting mixture was stirred at room temperature for 1.5 h. The reaction mixture was diluted with EtOAc (50 mL), washed with water (3 × 10 mL), and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with CHCl / MeOH (10:1) to give tert-butyl 3-{3-chloro-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl}azetidine-1-carboxylate (300 mg, 55.21%) as a white solid. LCMS (ESI) m / z: [M+H] + =326.

[0374] Step 3: Preparation of tert-butyl 3-[3-(2-hydroxyphenyl)-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl]azetidine-1-carboxylate [ka] To a solution of tert-butyl 3-{3-chloro-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl}azetidine-1-carboxylate (250 mg, 0.767 mmol, 1.00 equiv.), 2-hydroxyphenylboronic acid (317.57 mg, 2.301 mmol, 3 equiv.), and CsCO (500.11 mg, 1.534 mmol, 2 equiv.) in dioxane (1.5 mL) and HO (1.5 mL) was added BrettPhos Pd G (69.57 mg, 0.076 mmol, 0.1 equiv.) under a dry nitrogen atmosphere, and the resulting mixture was stirred at 80°C under a dry nitrogen atmosphere for 1.5 h. The solid was filtered off, the filtrate was concentrated under reduced pressure, and the residue was purified by flash C18 chromatography using an elution gradient of 0 to 80% MeCN (containing 0.1% NH4HCO3) in water. Pure fractions were evaporated to dryness to give tert-butyl 3-[3-(2-hydroxyphenyl)-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl]azetidine-1-carboxylate (48 mg, 16.31%) as a white solid. LCMS (ESI) m / z: [M+H] + =384

[0375] Step 4: Preparation of 5-(azetidin-3-yl)-3-(2-hydroxyphenyl)-7H-imidazo[4,5-c]pyridazin-6-one [ka] A solution of tert-butyl 3-[3-(2-hydroxyphenyl)-6-oxo-7H-imidazo[4,5-c]pyridazin-5-yl]azetidine-1-carboxylate (45 mg, 0.117 mmol, 1.00 equiv) in TFA (1 mL) and DCM (2 mL) was stirred at room temperature for 1 h. The resulting mixture was concentrated under reduced pressure to give intermediate 5 (20 mg, 60.15%) as a white solid, which was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =284

[0376] Preparation of 2-{6',7'-dihydrospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazin]-3'-yl}phenol (I-118) [ka] Step 1: Preparation of 3,6-dichloro-N-[(3,4-dimethylphenyl)methyl]pyridazin-4-amine [ka] To a stirred solution of 3,4,6-trichloropyridazine (10 g, 54.520 mmol, 1.00 equiv) in THF (50 mL) was added (2,4-dimethoxyphenyl)methanamine (27.35 g, 163.560 mmol, 3.0 equiv) dropwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 50°C for 2 hours. The resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give 3,6-dichloro-N-[(3,4-dimethylphenyl)methyl]pyridazin-4-amine (13 g, 84.50%) as a white solid. LCMS (ESI) m / z [M+H] + =578.

[0377] Step 2: Preparation of tert-butyl 8-[1-(tert-butoxycarbonyl)azetidine-3-carbonyl]-6,9-dichloro-11-[(3,4-dimethylphenyl)methyl]-12-oxo-2,7,8,11-tetraazadispiro[3.0.5^{5}.2^{4}]dodeca-6,9-diene-2-carboxylate [ka] A solution of 3,6-dichloro-N-[(3,4-dimethylphenyl)methyl]pyridazin-4-amine (600 mg, 2.126 mmol, 1.00 equiv) and tert-butyl 3-(carboxy)azetidine-1-carboxylate (4.67 g, 21.260 mmol, 10 equiv), EtN (2.15 g, 21.260 mmol, 10 equiv) in DCM (10 mL) was stirred at 25°C for 12 h. The resulting mixture was concentrated under reduced pressure to give tert-butyl 8-[1-(tert-butoxycarbonyl)azetidine-3-carbonyl]-6,9-dichloro-11-[(3,4-dimethylphenyl)methyl]-12-oxo-2,7,8,11-tetraazadispiro[3.0.5^{5}.2^{4}]dodeca-6,9-diene-2-carboxylate (4.7 g, crude) as a pale yellow solid. The crude product was used directly in the next step without further purification. LCMS (ESI) m / z [M+H] + =680.

[0378] Step 3: Preparation of tert-butyl 3'-chloro-7'-[(3,4-dimethylphenyl)methyl]-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate [ka] A solution of tert-butyl 8-[1-(tert-butoxycarbonyl)azetidine-3-carbonyl]-6,9-dichloro-11-[(3,4-dimethylphenyl)methyl]-12-oxo-2,7,8,11-tetraazadispiro[3.0.55.24]dodeca-6,9-diene-2-carboxylate (4.5 g, 6.938 mmol, 1.00 equiv.) and CsCO (1.81 g, 5.550 mmol, 0.80 equiv.) in DMF (10 mL) was stirred at 80 °C for 12 h under a dry nitrogen atmosphere. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, CHCN in water, gradient from 0% to 75% in 50 min; detector, UV at 254 nm. This gave tert-butyl 3'-chloro-7'-[(3,4-dimethylphenyl)methyl]-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (800 mg, 26.88%) as a yellow solid. LCMS (ESI) m / z [M+H] + =461.

[0379] Step 4: Preparation of tert-butyl 7'-[(3,4-dimethylphenyl)methyl]-3'-(2-hydroxyphenyl)-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate [ka] To a solution of tert-butyl 3'-chloro-7'-[(3,4-dimethylphenyl)methyl]-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (600 mg, 1.399 mmol, 1 equiv.) and 2-hydroxyphenylboronic acid (578.84 mg, 4.197 mmol, 3.0 equiv.) in dioxane (5 mL) and HO (1 mL) was added CsCO (1367.33 mg, 4.197 mmol, 3.0 equiv.) and XPhos Pd G (236.82 mg, 0.280 mmol, 0.2 equiv.). After stirring at 80 °C under a nitrogen atmosphere for 4 h, the resulting mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (1:1) to give tert-butyl 7'-[(3,4-dimethylphenyl)methyl]-3'-(2-hydroxyphenyl)-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (400 mg, 58.77%) as a pale yellow solid. LCMS (ESI) m / z [M+H] + =519.

[0380] Step 5: Preparation of 3'-(2-hydroxyphenyl)-7'H-spiro[azetidin-3,5'-pyrrolo[2,3-c]pyridazin]-6'-one [ka] A solution of tert-butyl 7'-[(3,4-dimethylphenyl)methyl]-3'-(2-hydroxyphenyl)-6'-oxospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazine]-1-carboxylate (400 mg, 0.822 mmol, 1 equiv.) and CFSOH (2 mL) in toluene (2 mL) was added under a dry nitrogen atmosphere at 120 °C for 4 h. The mixture was acidified to pH 6 with saturated NaHCO (aq.). The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, 0% to 100% gradient in 50 min; detector, UV 254 nm. This gave 3'-(2-hydroxyphenyl)-7'H-spiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazin]-6'-one (200 mg, 90.68%) as a pale yellow solid. LCMS (ESI) m / z [M+H] + =269.

[0381] Step 6: Preparation of 2-{6',7'-dihydrospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazin]-3'-yl}phenol [ka] To a stirred solution of 3'-(2-hydroxyphenyl)-7'H-spiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazin]-6'-one (100 mg, 0.373 mmol, 1.00 equiv) in THF (10 mL, 123.430 mmol, 331.13 equiv) was added LAH (42.44 mg, 1.119 mmol, 3.0 equiv) dropwise at 0°C under a dry nitrogen atmosphere. The reaction was quenched with saturated NH4Cl (aq) at 0°C. The resulting mixture was extracted with EtOAc (3 x 20 mL). The combined organic layers were dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeOH in water, gradient 0% to 50% in 25 min; detector, UV 254 nm. This gave 2-{6',7'-dihydrospiro[azetidine-3,5'-pyrrolo[2,3-c]pyridazin]-3'-yl}phenol (18 mg, 18.99%) as a yellow solid. LCMS (ESI) m / z [M+H] + =255.

[0382] Preparation of 2-[5-(azetidin-3-yl)-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-119) [ka] Step 1: Preparation of benzyl 3-(1-cyano-2-ethoxy-2-oxoethyl)azetidine-1-carboxylate [ka] To a stirred solution of benzyl 3-oxoazetidine-1-carboxylate (5.0 g, 24.365 mmol, 1.00 equiv) and ethyl cyanoacetate (4.13 g, 36.547 mmol, 1.5 equiv) in DMSO (10 mL) was added diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate (12.34 g, 48.730 mmol, 2 equiv) and (2S)-pyrrolidine-2-carboxylic acid (1.12 g, 9.746 mmol, 0.4 equiv) at room temperature. The resulting mixture was stirred at room temperature for 1 day. The desired product could be detected by LCMS. The resulting mixture was extracted with EtOAc (2 × 50 mL). The combined organic layers were washed with brine (2 × 20 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient 0% to 100% in 25 min; detector, UV 254 nm. This gave benzyl 3-(1-cyano-2-ethoxy-2-oxoethyl)azetidine-1-carboxylate (3.1 g, 42.08%) as a pale yellow solid. LCMS (ESI) m / z [M+H] + =303.

[0383] Step 2: Preparation of benzyl 3-(1-cyano-2-ethoxy-1-methyl-2-oxoethyl)azetidine-1-carboxylate [ka] To a stirred mixture of benzyl 3-(1-cyano-2-ethoxy-2-oxoethyl)azetidine-1-carboxylate (3 g, 9.923 mmol, 1.00 equiv.) and CsCO (9.70 g, 29.769 mmol, 3 equiv.) in DMF (5 mL) was added MeI (1.41 g, 9.923 mmol, 1 equiv.) at room temperature. The resulting mixture was stirred overnight at room temperature. The desired product could be detected by LCMS. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient from 0% to 100% in 25 min; detector 41%, UV 254 nm. The resulting mixture was concentrated under reduced pressure. This gave benzyl 3-(1-cyano-2-ethoxy-1-methyl-2-oxoethyl)azetidine-1-carboxylate (950 mg, 30.26%) as a tan solid. LCMS (ESI) m / z [M+H] + =317.

[0384] Step 3: Preparation of benzyl 3-{1-[(tert-butoxycarbonyl)amino]-3-hydroxy-2-methylpropan-2-yl}azetidine-1-carboxylate [ka] To a stirred solution of benzyl 3-(1-cyano-2-ethoxy-1-methyl-2-oxoethyl)azetidine-1-carboxylate (1.3 g, 4.109 mmol, 1 equiv) in THF (30 mL) was added BH3-THF (0.71 g, 8.218 mmol, 2.0 equiv) dropwise at 0 °C under a dry nitrogen atmosphere. The reaction was quenched with MeOH at 0 °C. The mixture was acidified to pH 13 with saturated NaHCO3 (aq). To the above mixture, Boc2O (3.59 g, 16.436 mmol, 4.0 equiv) was added dropwise at room temperature. The resulting mixture was stirred for an additional 1 h at room temperature. The resulting mixture was diluted with water (50 mL). The resulting mixture was extracted with EtOAc (3 × 100 mL). The combined organic layers were dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The crude product was used directly in the next step without further purification, which gave benzyl 3-{1-[(tert-butoxycarbonyl)amino]-3-hydroxy-2-methylpropan-2-yl}azetidine-1-carboxylate (2.1 g, crude) as a yellow oil. LCMS (ESI) m / z [M+H] + =379.

[0385] Step 4: Preparation of benzyl 3-{1-[(tert-butoxycarbonyl)amino]-2-methyl-3-oxopropan-2-yl}azetidine-1-carboxylate [ka] A solution of benzyl 3-{1-[(tert-butoxycarbonyl)amino]-3-hydroxy-2-methylpropan-2-yl}azetidine-1-carboxylate (2.0 g, 5.284 mmol, 1 equiv.) and DMP (13.45 g, 31.704 mmol, 6.0 equiv.) in DCM (20 mL) was stirred for 2 h at room temperature. The reaction was quenched with saturated NaSO (aq.) at room temperature. The resulting mixture was diluted with water (200 mL). The resulting mixture was extracted with CHCl (3 × 100 mL). The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. This afforded benzyl 3-{1-[(tert-butoxycarbonyl)amino]-2-methyl-3-oxopropan-2-yl}azetidine-1-carboxylate (1.9 g, 95.51%) as a yellow oil. The crude product was used directly in the next step without further purification. LCMS (ESI) m / z [M+H] + =377.

[0386] Step 5: Preparation of benzyl 3-(2-{[(tert-butoxycarbonyl)amino]methyl}but-3-yn-2-yl)azetidine-1-carboxylate [ka] A solution of benzyl 3-{1-[(tert-butoxycarbonyl)amino]-2-methyl-3-oxopropan-2-yl}azetidine-1-carboxylate (1.9 g, 5.047 mmol, 1 equiv) in MeOH (10 mL) was treated with K2CO3 (2.09 g, 15.141 mmol, 3.0 equiv) under a dry nitrogen atmosphere for 10 minutes at 0 °C, followed by the dropwise addition of dimethyl (diazomethyl)phosphonate (969.61 mg, 5.047 mmol, 1.0 equiv) at 0 °C. The resulting mixture was stirred for 4 hours at room temperature. The resulting mixture was diluted with water (100 mL). The resulting mixture was extracted with EtOAc (3 × 150 mL). The combined organic layers were dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient 0% to 100% in 50 min; detector, UV 254 nm. This gave benzyl 3-(2-{[(tert-butoxycarbonyl)amino]methyl}but-3-yn-2-yl)azetidine-1-carboxylate (500 mg, 26.60%) as a yellow oil. LCMS (ESI) m / z [M+H] + =373.

[0387] Step 6: Preparation of benzyl 3-(1-amino-2-methylbut-3-yn-2-yl)azetidine-1-carboxylate [ka] A solution of 3-(2-{[(tert-butoxycarbonyl)amino]methyl}but-3-yn-2-yl)azetidine-1-carboxylic acid (500 mg, 1.342 mmol, 1 equiv.) and TFA (1 mL) in DCM (4 mL) was stirred at room temperature for 2 h. The resulting mixture was concentrated under reduced pressure. The crude product was used directly in the next step without further purification. This gave benzyl 3-(1-amino-2-methylbut-3-yn-2-yl)azetidine-1-carboxylate (500 mg, 136.76%) as a yellow oil. LCMS (ESI) m / z [M+H] + =273.

[0388] Step 7: Preparation of benzyl 3-{3-chloro-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl}azetidine-1-carboxylate [ka] A solution of benzyl 3-(1-amino-2-methylbut-3-yn-2-yl)azetidine-1-carboxylate (500 mg, 1.836 mmol, 1.00 equiv.), DIEA (1423.66 mg, 11.016 mmol, 6.0 equiv.), and dichloro-1,2,4,5-tetrazine (554.25 mg, 3.672 mmol, 2.0 equiv.) in dioxane (10 mL) was stirred at 100°C for 12 hours under a dry nitrogen atmosphere. The resulting mixture was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, 0% to 50% gradient in 40 min; detector, UV 254 nm. This gave benzyl 3-{3-chloro-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl}azetidine-1-carboxylate (250 mg, 37.95%) as a tan oil. LCMS (ESI) m / z [M+H] + =359.

[0389] Step 8: Preparation of benzyl 3-[3-(2-hydroxyphenyl)-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylate [ka] To a solution of benzyl 3-{3-chloro-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl}azetidine-1-carboxylate (250 mg, 0.697 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (288.29 mg, 2.091 mmol, 3.0 equiv) in dioxane (4 mL) and HO (0.8 mL) was added CsCO (681.00 mg, 2.091 mmol, 3.0 equiv) and Pd(AMPHOS)Cl (98.66 mg, 0.139 mmol, 0.2 equiv). After stirring at 80°C under a nitrogen atmosphere for 2 hours, the resulting mixture was concentrated under reduced pressure. The resulting mixture was diluted with water (10 mL). The resulting mixture was extracted with EtOAc (3 × 10 mL). The combined organic layers were dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, silica gel; mobile phase, MeCN in water, gradient 0% to 100% in 50 min; detector, UV 254 nm. This afforded benzyl 3-[3-(2-hydroxyphenyl)-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylate (200 mg, 68.93%) as a yellow oil. LCMS (ESI) m / z [M+H] + =417.

[0390] Step 9: Preparation of 2-[5-(azetidin-3-yl)-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (I-119) [ka] To a stirred solution of benzyl 3-[3-(2-hydroxyphenyl)-5-methyl-6H,7H-pyrrolo[2,3-c]pyridazin-5-yl]azetidine-1-carboxylate (60 mg, 0.144 mmol, 1.00 equiv) in DCM (3 mL) was added BBr3 (360.91 mg, 1.440 mmol, 10 equiv) dropwise at 0 degrees Celsius. The crude product was purified by preparative HPLC under the following conditions (column: XBridge Prep C18 OBD column, 19*150 mm, 5 μm; mobile phase A: water (10 mmol / L NH4HCO3), mobile phase B: CH3CN; flow rate: 25 mL / min; gradient: 12% B to 28% B, 28% B in 6 min; wavelength: 254 / 220 nm) to give I-119 (12.9 mg, 30.26%) as a yellow solid. 1 H NMR(300MHz, methanol-d4)δ 8.09(s,1H),7.94(dd,J=8.3,1.6Hz,1H),7.36(ddd,J=8.7,7.2,1.6Hz,1H),7.09-6.97(m,2H),3.97- 3.80(m,3H),3.78-3.70(m,2H),3.56(d,J=10.0Hz,1H),3.52-3.40(m,1H),1.52(s,3H).LCMS(ESI)m / z [M+H] + =282.15.

[0391] Preparation of 2-(6-(azetidin-3-yl)-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-120) [ka] Step 1: Preparation of (E)-benzyl 3-(1-((tert-butylsulfinyl)imino)ethyl)azetidine-1-carboxylate [ka] To a stirred mixture of tert-butanesulfinamide (1.42 g, 11.716 mmol, 1.00 equiv) and benzyl 3-acetylazetidine-1-carboxylate (3.01 g, 12.888 mmol, 1.1 equiv) in THF, Ti(Oi-Pr)4 (6.66 g, 23.432 mmol, 2.0 equiv) was added portionwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 60°C for 5 hours under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The reaction was quenched at room temperature by adding water (20 mL). The precipitated solid was collected by filtration and washed with ethyl acetate (3 × 100 mL). The resulting mixture was extracted with EtOAc (300 mL). The combined organic layers were washed with deionized water (3 × 100 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. This gave benzyl (E)-3-(1-((tert-butylsulfinyl)imino)ethyl)azetidine-1-carboxylate (3.71 g, 94.12%) as a pale yellow oil. LCMS (ESI) m / z: [M+H] + =337.

[0392] Step 2: Preparation of benzyl 3-(2-((tert-butylsulfinyl)amino)-5-(trimethylsilyl)pentopent-4-yn-2-yl)azetidine-1-carboxylate [ka] To a stirred mixture of (3-bromoprop-1-yn-1-yl)trimethylsilane (1.77 g, 9.276 mmol, 6 equiv.) and Zn (606.56 mg, 9.276 mmol, 6 equiv.) in THF was added Intermediate 2 (520 mg, 1.546 mmol, 1.00 equiv.) dropwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred for an additional 16 hours at 50°C. The mixture was allowed to cool to room temperature. The resulting mixture was diluted with water (100 mL) and extracted with EtOAc (3 × 100 mL). The combined organic layers were washed with brine (100 mL) and dried over anhydrous NaSO. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeOH in water, 10% to 50% gradient in 10 min; detector, UV 254 nm. This gave benzyl 3-(2-((tert-butylsulfinyl)amino)-5-(trimethylsilyl)pent-4-yn-2-yl)azetidine-1-carboxylate (554.7 mg, 80.1%) as a pale yellow oil. LCMS (ESI) m / z: [M+H] + =449.

[0393] Step 3: Preparation of benzyl 3-(2-((tert-butylsulfinyl)amino)pent-4-yn-2-yl)azetidine-1-carboxylate [ka] A mixture of benzyl 3-(2-((tert-butylsulfinyl)amino)-5-(trimethylsilyl)pent-4-yn-2-yl)azetidine-1-carboxylate (1.0 g, 2.23 mmol, 1.00 equiv) and TBAF (2.91 g, 11.15 mmol, 5 equiv) in THF (50 mL) was stirred at room temperature for 2 hours. The reaction mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with PE / EA (2:1) to give benzyl 3-(2-((tert-butylsulfinyl)amino)pent-4-yn-2-yl)azetidine-1-carboxylate (755.4 mg, 90.0%) as a yellow oil. LCMS (ESI) m / z: [M+H] + =377.

[0394] Step 4: Preparation of benzyl 3-(2-aminopent-4-yn-2-yl)azetidine-1-carboxylate [ka] To a stirred mixture of benzyl 3-(2-((tert-butylsulfinyl)amino)pent-4-yn-2-yl)azetidine-1-carboxylate (930.0 mg, 2.47 mmol, 1.00 equiv) in DCM was added HCl (0.75 mL) and MeOH (10.00 mL) portionwise at room temperature. The resulting mixture was stirred for 1 h at room temperature. The mixture was neutralized to pH 7 with saturated NaHCO3 (aq). The resulting mixture was extracted with EtOAc (3 x 30 mL). The combined organic layers were washed with brine (1 x 40 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeOH in water, gradient 10% to 50% in 10 min; detector, UV 254 nm to give benzyl 3-(2-aminopent-4-yn-2-yl)azetidine-1-carboxylate (490 mg, 73.5%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =273.

[0395] Step 5: Preparation of benzyl 3-(3-chloro-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate [ka] To a stirred solution of benzyl 3-(2-aminopent-4-yn-2-yl)azetidine-1-carboxylate (490 mg, 1.799 mmol, 1.00 equiv) and dichloro-1,2,4,5-tetrazine (543.17 mg, 3.598 mmol, 2 equiv) in dioxane (20 mL) was added DIEA (697.59 mg, 5.397 mmol, 3 equiv) dropwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 100°C for 3 hours under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The resulting mixture was filtered, and the filter cake was washed with EtOAc (3 x 40 mL). The filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash chromatography under the following conditions (column: C18 silica gel; mobile phase: MeOH in water, gradient from 10% to 50% in 10 min; detector: UV 254 nm) to give benzyl 3-(3-chloro-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (500 mg, 77.45%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =359.

[0396] Step 6: Preparation of benzyl 3-(3-(2-hydroxyphenyl)-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate [ka] A mixture of benzyl 3-(3-chloro-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (200 mg, 0.557 mmol, 1.00 equiv.), XPhos Pd G3 (47.18 mg, 0.056 mmol, 0.1 equiv.), and Cs2CO3 (544.80 mg, 1.671 mmol, 3.0 equiv.) in dioxane (4 mL) and HO (0.5 mL) was stirred at 80°C for 4 hours under a dry nitrogen atmosphere. The mixture was allowed to cool to room temperature. The resulting mixture was diluted with water (50 mL) and extracted with EtOAc (3 × 50 mL). The combined organic layers were washed with brine (1 × 50 mL) and dried over anhydrous Na2SO4. After filtration, the filtrate was concentrated under reduced pressure. The residue was purified by reverse-phase flash under the following conditions (column: C18 silica gel; mobile phase: MeOH in water, gradient from 10% to 50% in 25 min; detector: UV 254 nm) to give benzyl 3-(3-(2-hydroxyphenyl)-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (100 mg, 43.08%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =417.

[0397] Step 7: Preparation of 2-(6-(azetidin-3-yl)-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-3-yl)phenol (I-120) [ka] To a solution of benzyl 3-(3-(2-hydroxyphenyl)-6-methyl-6,7-dihydro-5H-pyrrolo[2,3-c]pyridazin-6-yl)azetidine-1-carboxylate (20 mg, 0.048 mmol, 1 equiv.) in MeOH (1 mL) was added Pd(OH) / C (10%, 10 mg) in a 25 mL round-bottom flask under a dry nitrogen atmosphere. The mixture was hydrogenated under a hydrogen atmosphere at room temperature using a hydrogen balloon for 3 h, filtered through a Celite pad, and concentrated under reduced pressure. The crude product (21 mg) was purified by preparative HPLC under the following conditions (Column: XBridge Prep C18 OBD column, 19*150 mm, 5 μm; Mobile phase A: water (10 mmol / L NH4HCO3), Mobile phase B: CH3CN; Flow rate: 25 mL / min; Gradient: 5% B to 40% B, 40% B in 7 min; Wavelength: 254 / 220 nm; RT1 (min): 5.85; Run count: 0) to give I-120 (6 mg, 44.25%) as a white solid. 1 H NMR(300MHz, methanol-d4)δ 7.93(d,J=7.8Hz,1H),7.76(d,J=8.1Hz,1H),7.48-7.09(m,1H),7.09-6.70(m,2H) ,4.01-3.56(m,4H),3.26-2.94(m,3H),1.34(d,J=10.3Hz,3H).LCMS(ESI)m / z:[MH] - =283.25.

[0398] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-{3-[4-(2-{3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidin-1-yl}ethyl)piperazin-1-yl]-1,2-oxazol-5-yl}-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 273) [ka] (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (30.00 mg, 0.053 mmol) in CHCN (1.00 mL) To a stirred solution of 1,2-dibromoethane (9.94 mg, 0.053 mmol, 1.00 equiv.) and 2-[6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (42.29 mg, 0.159 mmol, 3.00 equiv.) was added 1,2-dibromoethane (9.94 mg, 0.053 mmol, 1.00 equiv.) and DIEA (41.05 mg, 0.318 mmol, 6.00 equiv.) at room temperature. The resulting mixture was stirred at 70°C for 2 hours. The mixture was allowed to cool to room temperature. The crude product was purified by preparative HPLC under the following conditions: column, XBridge Shield RP18 OBD column, 19*150 mm, 5 μm; mobile phase, water (10 mmol / L NH4HCO3) and MeOH (from 60% MeOH to 77% in 8 min); detector, UV 254 / 220 nm, to give the title compound (2.1 mg, 4.39%) as a yellow solid. 1 H NMR (400 MHz, DMSO-d 6)δ 14.16(s,1H),12.76-12.19(m,1H),8.98(s,1H),8.55(s,1H),8.39(d,J=7.7Hz, 1H),8.03(d,J=7.7Hz,1H),7.49-7.41(m,2H),7.37(d,J=8.2Hz,2H),7.33-7.26( m,1H),6.95(t,J=8.2Hz,2H),6.54(d,J=12.9Hz,1H),6.14(s,1H),5.10(d,J=3. 7Hz,1H),5.01-4.86(m,1H),4.36(t,J=7.8Hz,1H),4.31-4.24(m,1H),3.91-3.81 (m,1H),3.75-3.64(m,3H),3.57(d,J=10.0Hz,1H),3.51(s,1H),3.46-3.39(m,1 H),3.30-3.24(m,2H),3.20-3.12(m,4H),2.63(t,J=7.0Hz,2H),2.46(s,3H),2.3 7-2.33(m,1H),2.27-2.11(m,1H),2.06-1.96(m,1H),1.84-1.72(m,1H),1.38(d, J=7.0Hz,3H),0.96(t,J=6.6Hz,3H),0.81(d,J=6.6Hz,3H).LCMS(ESI)m / z:[M+H] + =859.50.

[0399] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(2-{4-[3-(2-hydroxyphenyl)-5-methyl-7H-pyrrolo[2,3-c]pyridazin-6-yl]piperidin-1-yl}ethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 270) [ka] Step 1: Preparation of tert-butyl 4-(prop-1-yn-1-yl)piperidine-1-carboxylate (Intermediate 2) [ka] To a stirred solution of tert-butyl 4-ethynylpiperidine-1-carboxylate (2 g, 9.556 mmol, 1.00 equiv) in THF (30 mL) was added LiHMDS (4.80 g, 28.668 mmol, 3.00 equiv) dropwise at −78° C. under a dry nitrogen atmosphere. The resulting mixture was stirred at −78° C. for 1 hour under a dry nitrogen atmosphere. To the above mixture was added MeI (6.78 g, 47.780 mmol, 5 equiv) dropwise at −78° C. The resulting mixture was stirred overnight at room temperature. The residue was purified by silica gel column chromatography eluting with PE / EA (9:1) to give intermediate 2 (1.3 g, 60.92%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =224.

[0400] Step 2: Preparation of tert-butyl 4-[3-chloro-5-methyl-7H-pyrrolo[2,3-c]pyridazin-6-yl]piperidine-1-carboxylate (Intermediate 3A) [ka] To a stirred solution of Intermediate 2 (1.2 g, 5.374 mmol, 1.00 equiv) and 4-bromo-6-chloropyridazin-3-amine (1.68 g, 8.061 mmol, 1.50 equiv) in DMF (30 mL), Pd(OAc) (241.28 mg, 1.075 mmol, 0.20 equiv), LiCl (227.81 mg, 5.374 mmol, 1.00 equiv), and NaCO (2.85 g, 26.870 mmol, 5.00 equiv) were added at room temperature under a dry nitrogen atmosphere. The reaction was stirred overnight at 120 °C. The crude product was purified by reverse-phase flash chromatography to give Intermediate 3B (140 mg, 7.42%) and Intermediate 3A (340 mg, 18.03%) as a yellow solid. LCMS(ESI)m / z:[M+H] + =351.

[0401] Step 3: Preparation of tert-butyl 4-[3-(2-hydroxyphenyl)-5-methyl-7H-pyrrolo[2,3-c]pyridazin-6-yl]piperidine-1-carboxylate (Intermediate 4) [ka] To a stirred mixture of Intermediate 3A (320.00 mg, 0.912 mmol, 1.00 equiv) and 2-hydroxyphenylboronic acid (251.60 mg, 1.824 mmol, 2.00 equiv) in dioxane (6.0 mL) and HO (1.5 mL), CsCO (891.51 mg, 2.736 mmol, 3.00 equiv) and XPhos Pd G (154.40 mg, 0.182 mmol, 0.20 equiv) were added portionwise at room temperature under a dry nitrogen atmosphere. The resulting mixture was stirred at 90 °C for 2 h. The residue was purified by reverse-phase flash chromatography to give Intermediate 4 (240 mg, 64.42%) as a yellow solid. LCMS (ESI) m / z: [M+H] + =409.

[0402] Step 4: Preparation of 2-[5-methyl-6-(piperidin-4-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (Intermediate 5) [ka] To a stirred solution of intermediate 4 (100 mg, 0.245 mmol, 1 equiv) in DCM (2.0 mL) was added TFA (0.5 mL) dropwise at room temperature. The resulting mixture was stirred at room temperature for 1 h. The resulting mixture was concentrated under reduced pressure. The crude product 5 was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =309.

[0403] Step 5: Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(2-{4-[3-(2-hydroxyphenyl)-5-methyl-7H-pyrrolo[2,3-c]pyridazin-6-yl]piperidin-1-yl}ethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 270) [ka] To a stirred solution of Intermediate 5 (11.41 mg, 0.036 mmol, 2 equiv.) and (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(2-oxoethoxy)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (10 mg, 0.018 mmol, 1.00 equiv.) in MeOH (1.0 mL) and DCM (1.0 mL), AcOH (catalytic) and NaBHCN (5.81 mg, 0.090 mmol, 5 equiv.) were added portionwise at room temperature. The resulting mixture was stirred overnight at room temperature. The resulting mixture was concentrated under reduced pressure. The crude product was purified by preparative HPLC to give compound 270 (3.0 mg, 19.47%) as a yellow solid. 1 H NMR(400MHz, methanol-d4)δ 8.87(s,1H),8.37(s,1H),8.02-7.91(m,1H),7.51-7.38(m,3H),7.35(s ,1H),7.27(ddd,J=8.6,7.3,1.6Hz,1H),6.96(dtt,J=7.1,4.7,2.3Hz,2H ),6.04(s,1H),5.03(q,J=7.0Hz,1H),4.52(t,J=8.2Hz,1H),4.48-4.36( m,3H),3.85(dd,J=10.9,4.2Hz,1H),3.79-3.65(m,1H),3.64-3.60(m,1H ),3.59-3.45(m,1H),3.20(d,J=11.2Hz,2H),3.06(s,1H),2.90(t,J=5. 3Hz,2H),2.47(s,2H),2.41(s,1H),2.34(d,J=9.5Hz,6H),2.24-2.13(m, 1H),2.09-1.91(m,3H),1.88(d,J=12.6Hz,2H),1.60-1.51(m,3H),1.06(dd,J=6.6,1.8Hz,3H),0.91(dd,J=8.8,6.7Hz,3H).LCMS(ESI)m / z:[M+H] + =834.2.

[0404] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(2-{4-[3-(2-hydroxyphenyl)-6-methyl-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}ethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 265) [ka] Step 1: Preparation of 2-[6-methyl-5-(piperidin-4-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (Intermediate 2) [ka] To a stirred solution of tert-butyl 4-(3-(2-hydroxyphenyl)-6-methyl-7H-pyrrolo[2,3-c]pyridazin-5-yl)piperidine-1-carboxylate (20 mg, 0.049 mmol, 1 equiv.) in DCM (1.0 mL) was added TFA (0.25 mL) dropwise at room temperature. The resulting mixture was stirred at room temperature for 1 h. The resulting mixture was concentrated under reduced pressure. The crude product 2 was used directly in the next step without further purification. LCMS (ESI) m / z: [M+H] + =309.

[0405] Step 2: Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(2-{4-[3-(2-hydroxyphenyl)-6-methyl-7H-pyrrolo[2,3-c]pyridazin-5-yl]piperidin-1-yl}ethoxy)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 265) [ka] To a stirred solution of intermediate 2 (14.03 mg, 0.046 mmol, 2 equiv) and (2S,4R)-4-hydroxy-1-((R)-3-methyl-2-(3-(2-oxoethoxy)isoxazol-5-yl)butanoyl)-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (12.3 mg, 0.023 mmol, 1 equiv) in MeOH (1.0 mL) and DCM (1.0 mL) was added AcOH (catalytic) and NaBHCN (7.1 mg, 0.113 mmol, 5 equiv) in portions at room temperature. The resulting mixture was stirred overnight at room temperature. The resulting mixture was concentrated under reduced pressure. The crude product was purified by preparative HPLC to give compound 265 (2.3 mg, 4.26%) as a yellow solid. 1 H NMR(300MHz, methanol-d4)δ 8.88(d,J=4.0Hz,1H),8.75(d,J=4.0Hz,1H),8.08(d,J=8.4Hz,1H),7.51-7.35(m,4H),7.35-7.24(m,1H),6.99(q,J=7.4,6.7Hz,2H),6. 09(s,1H),5.05(d,J=7.1Hz,1H),4.59-4.48(m,1H),4.47-4.39(m,3H),3.93-3.81(m,1H),3.71(d,J=9.9Hz,1H),3.65(d,J=1.9Hz,3H), 3.64-3.47(m,1H),3.24(d,J=7.8Hz,2H),2.94(t,J=5.2Hz,2H),2.56-2.51(m,3H),2.50-2.48(m,2H),2.47-2.28(m,6H),2.19(dd,J=14 .4,7.2Hz,1H),2.10-1.75(m,4H),1.62-1.58(m,1H),1.56-1.50(m,3H),1.19-1.04(m,3H),0.93(t,J=6.9Hz,3H).LCMS(ESI)m / z:[M+H] + =834.40.

[0406] The compounds in Table 18 were prepared using procedures similar to those used above for the preparation of compound 265 using the appropriate amine and aldehyde. [Table 19-1] [Table 19-2] [Table 19-3] [Table 19-4] [Table 19-5] [Table 19-6] [Table 19-7] [Table 19-8] [Table 19-9] [Table 19-10]

[0407] Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(4-{3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carbonyl}piperazin-1-yl)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide (Compound 124) [ka] Step 1: Preparation of 4-nitrophenyl 3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carboxylic acid [ka] To a stirred solution of 2-[6-(azetidin-3-yl)-7H-pyrrolo[2,3-c]pyridazin-3-yl]phenol (100.00 mg, 0.376 mmol, 1.00 equiv) in pyridine (5.00 mL) was added 4-nitrophenyl carbonochloridate (151.38 mg, 0.752 mmol, 2.00 equiv) at 0°C. The resulting mixture was stirred at room temperature for 16 hours. The resulting mixture was concentrated in vacuo. The residue was purified by reverse-phase flash chromatography under the following conditions: column, C18 silica gel; mobile phase, MeCN (0.1% FA) in water, gradient 0% to 100% in 30 minutes; detector, UV 254 / 220 nm. This afforded intermediate 2 (59 mg, 36.42%) as a brown solid. LCMS (ESI) m / z: [M+H] + =432.

[0408] Step 2: Preparation of (2S,4R)-4-hydroxy-1-[(2R)-2-[3-(4-{3-[3-(2-hydroxyphenyl)-7H-pyrrolo[2,3-c]pyridazin-6-yl]azetidine-1-carbonyl}piperazin-1-yl)-1,2-oxazol-5-yl]-3-methylbutanoyl]-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide [ka] To a stirred solution of Intermediate 2 (59.00 mg, 0.095 mmol, 1.00 equiv) in pyridine (5.00 mL) was added (2S,4R)-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]-1-[(2R)-3-methyl-2-[3-(piperazin-1-yl)-1,2-oxazol-5-yl]butanoyl]pyrrolidine-2-carboxamide (53.86 mg, 0.095 mmol, 1.00 equiv) at room temperature. The resulting mixture was stirred at 100°C for 16 hours. The mixture was allowed to cool to room temperature. The resulting mixture was concentrated under reduced pressure. The crude product was purified by preparative HPLC under the following conditions: column, XBridge Prep Phenyl OBD column. Purification was performed using a 19*150 mm column, 5 μm mobile phase: water (10 mmol / L NH4HCO3) and ACN (from 36% ACN to 46% in 7 min); detector: UV 254 / 220 nm. This afforded compound 124 (1.00 mg, 1.13%) as a white solid. 1 H NMR (300 MHz, DMSO-d 6 )δ 14.12-14.04(m,1H),12.78-12.60(m,1H),8.99(s,1H),8.59(s,1H),8.43-8.31(m,1H),8.08-8.01(m,1H),7.49-7.41(m,2H),7.4 1-7.34(m,2H),7.34-7.26(m,1H),7.01-6.92(m,2H),6.66(s,1H),6.19(s,1H),5.13-5.07(m,1H),4.98-4.86(m,1H),4.44-4.24(m ,4H),4.24-4.07(m,3H),3.77-3.67(m,1H),3.65-3.53(m,1H),3.53-3.35(m,5H),3.23-3.12(m,4H),2.46(s,3H),2.26-2.11(m,1 H),2.10-1.96(m,1H),1.86-1.73(m,1H),1.39(d,J=7.1Hz,3H),0.96(d,J=6.7Hz,3H),0.80(d,J=6.7Hz,3H).LCMS(ESI)m / z:[M+H] + =859.60.

[0409] Example 5. Degradation of BRM and BRG1 by compounds of the present invention This example demonstrates the ability of compounds of the present disclosure to degrade HiBit-BRM or HiBit-BRG1 fusion proteins in a cell-based degradation assay.

[0410] Procedure: A stable HeLa cell line expressing HiBiT-BRM was generated. On day 0, 5,000 cells were seeded in 40 μL of medium into each well of a 384-well cell culture plate. On day 1, cells were treated with 120 nL of DMSO or 120 nL of 3-fold serial DMSO dilutions of compound (30 μM final highest dose, 10 duplicates). Plates were then incubated for 24 hours in a standard tissue culture incubator and equilibrated at room temperature for 15 minutes. Nano-Glo HiBiT Lytic Detection System (Promega N3050) reagent was freshly prepared and 20 μL was added to each well. Upon addition of this LgBit-containing reagent, HiBiT and LgBiT proteins associate to form the luminescent NanoBiT luciferase. Plates were shaken at room temperature for 10 minutes, and bioluminescence was read using an EnVision plate reader (PerkinElmer).

[0411] To measure BRG1 degradation, we generated a stable HeLa cell line expressing HiBit-BRG1 and LgBit, and then followed the same protocol as above.

[0412] The % decomposition was calculated using the following formula: % decomposition = 100% - 100% × (Lum Sample -Lum LC ) / (Lum HC -Lum LC ) were used to calculate the IC. Cells treated with DMSO were used as the high control (HC), and standard treatment with 2 μM of a known BRM / BRG1 degrader was used as the low control (LC). The data were fitted with a four-parameter nonlinear curve fit to calculate the IC, as shown in Table 19. 50 (μm) values were calculated.

[0413] Results: As shown in Table 19 below, the compounds of the present invention degraded BRM and / or BRG1. [Table 20-1] [Table 20-2] [Table 20-3] [Table 20-4] [Table 20-5] [Table 20-6] [Table 20-7] [Table 20-8] [Table 20-9] [Table 20-10] [Table 20-11]

[0414] Other embodiments All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference in its entirety. In the event that a term in this application is found to be defined differently in a document incorporated herein by reference, the definition provided herein shall serve as the definition of that term.

[0415] While the invention has been described in relation to particular embodiments thereof, it will be understood that the invention is capable of further modifications, and this application is intended to cover any variations, uses, or adaptations of the invention which generally follow the principles of the invention and include departures from the present disclosure which come within known or customary practice within the art to which this invention pertains, and which may be applied to the essential features set forth herein above, and which fall within the scope of the appended claims.

[0416] Other embodiments are within the scope of the following claims. The inventions described in the original claims of this application are set forth below. [1] A compound having the structure of Formula I, or a pharmaceutically acceptable salt thereof: [ka] [In the formula, Ring system A is a 5- to 9-membered heterocyclyl or heteroaryl; m is 0, 1, 2, or 3; k is 0, 1, or 2; Each R 1 are independently halo, optionally substituted C 1 -C 6 Alkyl or optionally substituted C 3 -C 8 is cycloalkyl, R 2 is H or optionally substituted C 1 -C 6 is alkyl, each X is independently halo; L is a linker, B is the decomposition part]. [2] The compound has the structure of formula IA [ka] [wherein the broken bond represents a single bond or a double bond], the compound according to [1] or a pharmaceutically acceptable salt thereof. [3] The compound of [1], or a pharmaceutically acceptable salt thereof, wherein the compound has the structure of formula IB: [ka] [4] The compound has the structure of formula IC

change

Claims

1. A compound having the structure of Formula I, or a pharmaceutically acceptable salt thereof: 【Chemical 1】 [In the formula, Ring system A is a 5- to 9-membered heterocyclyl or heteroaryl; m is 0, 1, 2, or 3; k is 0, 1, or 2; Each R １ are independently selected from halo, optionally substituted C １ -C ６ alkyl, or optionally substituted C ３ -C ８ is cycloalkyl, R ２ is H or optionally substituted C １ -C ６ is alkyl, each X is independently halo; L is a linker, B is a decomposition moiety. wherein the compound has the structure of Formula IG or Formula IH: 【Chemistry 2】 The decomposition moiety B has the structure of formula A-1: 【Chemistry 3】 [In the formula, Y １ teeth, 【Chemistry 4】 and R Ａ５ is H, optionally substituted C １ -C ６ alkyl, or optionally substituted C １ -C ６ is heteroalkyl, R Ａ６ is H or optionally substituted C １ -C ６ alkyl, and R Ａ７ is H or optionally substituted C １ -C ６ alkyl or R Ａ６ and R Ａ７ Each, together with the carbon atom to which it is attached, represents an optionally substituted C ３ -C ６ Carbocyclyl or optionally substituted C ２ -C ５ forming a heterocyclyl, or R Ａ６ and R Ａ７ Each, together with the carbon atom to which it is attached, represents an optionally substituted C ３ -C ６ Carbocyclyl or optionally substituted C ２ -C ５ forming a heterocyclyl, R Ａ８ is H, optionally substituted C １ -C ６ alkyl, or optionally substituted C １ -C ６ is heteroalkyl, R Ａ１ , R Ａ２ , R Ａ３ , and R Ａ４ Each of the groups independently represents H, A ２ , halogen, optionally substituted C １ -C ６ Alkyl, optionally substituted C １ -C ６ Heteroalkyl, optionally substituted C ３ -C １０ Carbocyclyl, optionally substituted C ２ -C ９ Heterocyclyl, optionally substituted C ６ -C １０ Aryl, optionally substituted C ２ -C ９ Heteroaryl, optionally substituted C ２ -C ６ Alkenyl, optionally substituted C ２ -C ６ Heteroalkenyl, optionally substituted —O—C ３ -C ６ carbocyclyl, hydroxyl, thiol, or optionally substituted amino, or R Ａ１ and R Ａ２ , R Ａ２ and R Ａ３ , and / or R Ａ３ and R Ａ４ together with the carbon atoms to which they are attached, 【Chemistry 5】 It forms 【Chemistry 6】 is an optionally substituted C ６ -C １０ aryl, optionally substituted C ３ -C １０ Carbocyclyl, optionally substituted C ２ -C ９ Heteroaryl, or C ２ -C ９ heterocyclyl, any of which is optionally A ２ is replaced by R Ａ１ , R Ａ２ , R Ａ３ , and R Ａ４ One of them is A ２ or 【Chemistry 7】 A ２ is replaced by A ２ is the bond between the degradation moiety and the linker, The linker L has the following structure: 【Chemistry 8-1】 【Chemistry 8-2】 【Chemistry 8-3】 The compound or a pharmaceutically acceptable salt thereof.

2. R Ａ５ is H or methyl, and R Ａ１ , R Ａ２ , R Ａ３ and R Ａ４ each independently represents H or A ２ 2. The compound of claim 1, wherein:

3. Y １ 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein: 【Chemistry 9】

4. R Ａ６ is H or R Ａ７ is H or R Ａ６ is H and R Ａ７ or a pharmaceutically acceptable salt thereof.

5. Y １ 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein: 【Chemistry 10】

6. R Ａ８ is H or optionally substituted C １ -C ６ 6. The compound of claim 5, or a pharmaceutically acceptable salt thereof, wherein R is alkyl.

7. 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the degrading moiety is: 【Chemistry 11】

8. 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the degrading moiety is: 【Chemistry 12】

9. 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the degrading moiety comprises a structure of Formula A5, Formula A6, Formula A8, or Formula A10. 【Chemistry 13】

10. 2. The compound of claim 1, wherein the degrading moiety comprises the following structure: or a pharmaceutically acceptable salt thereof. 【Chemistry 14】

11. A compound selected from the group consisting of: 【Table 1-1】 【Table 1-2】 【Table 1-3】 【Table 1-4】 【Table 1-5】 【Table 1-6】 【Table 1-7】 【Table 1-8】 【Table 1-9】 【Table 1-10】 【Table 1-11】 【Table 1-12】 【Table 1-13】 【Table 1-14】 【Table 1-15】 【Table 1-16】 【Table 1-17】 【Table 1-18】 【Table 1-19】 【Table 1-20】 【Table 1-21】 【Table 1-22】 【Table 1-23】 【Table 1-24】 【Table 1-25】 【Table 1-26】 【Table 1-27】 【Table 1-28】 【Table 1-29】 【Table 1-30】 【Table 1-31】 【Table 1-32】 【Table 1-33】 【Table 1-34】 【Table 1-35】 【Table 1-36】 【Table 1-37】 【Table 1-38】 【Table 1-39】 【Table 1-40】 【Table 1-41】 【Table 1-42】 【Table 1-43】 【Table 1-44】 【Table 1-45】 【Table 1-46】 【Table 1-47】 【Table 1-48】 【Table 1-49】 【Table 1-50】 【Table 1-51】 【Table 1-52】 【Table 1-53】 【Table 1-54】 【Table 1-55】 【Table 1-56】 【Table 1-57】 【Table 1-58】 【Table 1-59】 【Table 1-60】 【Table 1-61】 【Table 1-62】 【Table 1-63】 【Table 1-64】 【Table 1-65】 【Table 1-66】 【Table 1-67】 【Table 1-68】 【Table 1-69】 【Table 1-70】 【Table 1-71】 【Table 1-72】 【Table 1-73】 【Table 1-74】 【Table 1-75】 【Table 1-76】 【Table 1-77】 【Table 1-78】 【Table 1-79】 【Table 1-80】 【Table 1-81】 【Table 1-82】 【Table 1-83】 【Table 1-84】 【Table 1-85】 【Table 1-86】 【Table 1-87】 Table 1-88 【Table 1-89】 【Table 1-90】 【Table 1-91】 【Table 1-92】 【Table 1-93】 【Table 1-94】 【Table 1-95】 【Table 1-96】 【Table 1-97】 【Table 1-98】 【Table 1-99】 【Table 1-100】 【Table 1-101】 【Table 1-102】 【Table 1-103】 【Table 1-104】 【Table 1-105】 【Table 1-106】 Table 1-107 【Table 1-108】 【Table 1-109】 【Table 1-110】 【Table 1-111】 【Table 1-112】 【Table 1-113】 【Table 1-114】 【Table 1-115】 【Table 1-116】 【Table 1-117】 【Table 1-118】 【Table 1-119】 (In the table above, the upper double dashed bond indicates a π-aromatic bond).

12. The compound is a BRG1 IC ５０ and BRM IC ５０ 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein the ratio of

13. A pharmaceutical composition comprising a compound according to any one of claims 1 to 12 and a pharmaceutically acceptable excipient.

14. A pharmaceutical composition for treating a BAF complex-associated disorder in a subject in need thereof, comprising an effective amount of a compound described in claim 1, wherein the BAF complex-associated disorder is cancer or a viral infection.

15. A pharmaceutical composition for treating a disorder associated with a loss-of-function mutation of BRG1 in a subject in need thereof, comprising an effective amount of a compound described in claim 1, wherein the disorder associated with a loss-of-function mutation of BRG1 is cancer.

16. 10. A pharmaceutical composition for treating cancer in a subject in need thereof, comprising an effective amount of a compound of claim 1.

17. 17. The pharmaceutical composition of claim 16, wherein the cancer is non-small cell lung cancer, colorectal cancer, bladder cancer, cancer of unknown primary site, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, esophagogastric cancer, pancreatic cancer, hepatobiliary cancer, soft tissue sarcoma, ovarian cancer, head and neck cancer, renal cell carcinoma, bone cancer, non-Hodgkin's lymphoma, small cell lung cancer, prostate cancer, embryonal tumor, germ cell tumor, cervical cancer, thyroid cancer, salivary gland cancer, gastrointestinal neuroendocrine tumor, uterine sarcoma, gastrointestinal stromal tumor, CNS cancer, thymic tumor, adrenocortical carcinoma, appendix cancer, small intestine cancer, or penile cancer.

18. 17. The pharmaceutical composition of claim 16, wherein the cancer is non-small cell lung cancer, colorectal cancer, bladder cancer, cancer of unknown primary, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, or penile cancer.

19. 10. A pharmaceutical composition for treating a cancer selected from the group consisting of melanoma, prostate cancer, breast cancer, bone cancer, renal cell carcinoma, and blood cancer, comprising an effective amount of a compound of claim 1.

20. 17. The pharmaceutical composition of claim 16, wherein the pharmaceutical composition is used in combination with an anti-cancer therapy, wherein the anti-cancer therapy is a chemotherapeutic or cytotoxic agent, immunotherapy, surgery, radiation therapy, hyperthermia, or photocoagulation.

21. 21. The pharmaceutical composition of claim 20, wherein the anti-cancer therapy is a chemotherapeutic or cytotoxic agent, and the chemotherapeutic or cytotoxic agent is an antimetabolite, an antimitotic, an antitumor antibiotic, an asparagine-specific enzyme, a bisphosphonate, an anti-neoplastic agent, an alkylating agent, a DNA repair enzyme inhibitor, a histone deacetylase inhibitor, a corticosteroid, a demethylating agent, an immunomodulator, a Janus-related kinase inhibitor, a phosphinositide 3-kinase inhibitor, a proteasome inhibitor, or a tyrosine kinase inhibitor.

22. 22. The pharmaceutical composition of claim 21, wherein the cancer is a blood cancer or melanoma, and the blood cancer is selected from the group consisting of multiple myeloma, large cell lymphoma, acute T-cell leukemia, acute myeloid leukemia, myelodysplastic syndrome, immunoglobulin A lambda myeloma, diffuse mixed histiocytic and lymphocytic lymphoma, B-cell lymphoma, acute lymphoblastic leukemia, diffuse large cell lymphoma, and non-Hodgkin's lymphoma, and the melanoma is selected from the group consisting of uveal melanoma, mucosal melanoma, and cutaneous melanoma.

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