Electrophoresis system, electrophoresis analysis method, and electrophoresis analysis program

Abstract

To provide an electrophoresis system, an electrophoretic analysis method, and an electrophoretic analysis program that, when an abnormality occurs in a measured value measured by electrophoresis, can reduce a workload on a worker for discriminating the cause of the abnormality.SOLUTION: An electrophoresis system 100 comprises an electrophoretic apparatus 101 including a measurement unit 30 that measures an object to be measured separated by electrophoresis, and a current detection unit 21 that detects a current caused to flow in a channel 61. The electrophoresis system 100 is configured to display, on a display 52 while the object to be measured is measured, a time-series value of a measured value 111 of the object to be measured which is measured by the measurement unit 30 and a time-series value of a current detection value 112 of the current detected by the current detection unit 21.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an electrophoresis system for performing electrophoresis, an electrophoretic analysis method, and an electrophoretic analysis program. [Background technology]

[0002] BACKGROUND ART Conventionally, an electrophoresis system for performing electrophoresis is known (see, for example, Patent Document 1).

[0003] The electrophoresis system described in Patent Document 1 includes an electrophoresis apparatus and an electrophoretic analysis apparatus. In the electrophoresis apparatus of this electrophoresis system, a DC voltage is applied to electrodes inserted into electrode chambers provided at both ends of a capillary, which is a flow path through which a sample to be measured flows to perform electrophoresis. When the DC voltage is applied to the electrodes to start electrophoresis, the sample moves by electrophoresis. The capillary is then monitored through a detection window, and actual waveform data showing changes over time in the fluorescence intensity from the moving sample is created and output to the electrophoretic analysis apparatus. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] International Publication No. 2018 / 181432 Summary of the Invention [Problem to be solved by the invention]

[0005] Although not described in Patent Document 1, if an abnormality occurs during measurement by electrophoresis, the measurement accuracy will be insufficient, resulting in an abnormal measurement result. Therefore, when an abnormality occurs in the measurement result (measurement value) of the electrophoresis measurement, the operator performing the measurement must check each part that is thought to be the cause of the abnormal measurement value. Therefore, when an abnormality occurs in the measured measurement value, the checking work to determine the cause of the abnormality becomes a burden on the operator.

[0006] The present invention has been made to solve the above-mentioned problems, and one object of the present invention is to provide an electrophoresis system, an electrophoretic analysis method, and an electrophoretic analysis program that can reduce the workload of an operator in determining the cause of an abnormality when an abnormality occurs in a measurement value measured by electrophoresis. [Means for solving the problem]

[0007] In order to achieve the above object, an electrophoresis system according to a first aspect of the present invention includes a separation channel for separating a measurement object. and a preparation channel for guiding the measurement object to the separation channel. and a current detection unit that detects a current flowing through the flow path, and wherein while the measurement of the measurement object is being performed in the separation flow path, In the preparation channel While the object to be measured is being guided into the separation flow path, the display unit is configured to display the time series values ​​of the measurement value of the object to be measured measured by the measurement unit and the time series values ​​of the current detection value of the current detected by the current detection unit.

[0008] The electrophoretic analysis method according to a second aspect of the present invention includes a separation channel for separating the measurement object. and a preparation channel for guiding the measurement object to the separation channel. a step of obtaining a measurement value of a measurement object separated by electrophoresis in a flow path including the flow path, a step of obtaining a current detection value of a current flowing in the flow path, and during the measurement of the measurement object in the separation flow path, In the preparation channeland displaying the time series values ​​of the measurement values ​​and the time series values ​​of the detected current values ​​on the display unit while the measurement object is being guided into the separation flow path.

[0009] In a third aspect of the present invention, the electrophoretic analysis program includes a separation flow path for separating the measurement object. and a preparation channel for guiding the measurement object to the separation channel. a step of obtaining a measurement value of a measurement object separated by electrophoresis in a flow path including the flow path, a step of obtaining a current detection value of a current flowing in the flow path, and during the measurement of the measurement object in the separation flow path, In the preparation channel and displaying, on a display unit, time-series values ​​of the measured values ​​and time-series values ​​of the detected current values ​​while the measurement object is being guided into the separation flow path. [Effects of the Invention]

[0010] In the electrophoresis system according to the first aspect, the electrophoresis analysis method according to the second aspect, and the electrophoresis analysis program according to the third aspect, a display unit displays time-series values ​​of measured values ​​and time-series values ​​of detected current during measurement of a measurement object. If an abnormality in the measured values ​​occurs and an abnormality in the current also occurs, this may be due to an abnormality in the concentration of the measurement object itself, an abnormality in the power supply for supplying the current, or an abnormality in the process of filling the flow path with the measurement object. On the other hand, if an abnormality in the measured values ​​occurs but no abnormality in the current occurs, this may be due to deterioration of the components forming the flow path. Therefore, by displaying time-series values ​​of measured values ​​and time-series values ​​of detected current during measurement of a measurement object, as described above, it is possible to visually check the display unit to determine whether an abnormality has occurred in the measured values ​​or the current. Therefore, if an abnormality occurs in a measurement value, it is possible to check whether an abnormality occurs in the current, and the cause of the abnormality in the measurement value can be narrowed down to possible abnormalities such as an abnormality in the concentration of the object to be measured itself, an abnormality in the power supply for passing the current, or an abnormality in the process of filling the flow path with the object to be measured, as well as possible deterioration of the materials forming the flow path.As a result, if an abnormality occurs in a measurement value measured by electrophoresis, the workload on the operator for determining the cause of the abnormality can be reduced. [Brief explanation of the drawings]

[0011] [Figure 1] 1 is a block diagram showing the overall configuration of an electrophoresis system according to an embodiment of the present invention. [Figure 2] FIG. 1 is a schematic diagram illustrating the configuration of an electrophoretic device according to an embodiment of the present invention. [Figure 3] FIG. 1 is a diagram illustrating the configuration of a chip provided with a channel for electrophoresis. [Figure 4] 5A and 5B are diagrams showing examples of measurement values ​​acquired by a measurement unit. [Figure 5] 10 is a diagram showing an example of a detected current value of a current flowing through a flow path. FIG. [Figure 6] FIG. 10 is a diagram showing an example of a detected voltage value of a voltage applied to a flow path. [Figure 7] FIG. 10 is a diagram showing an example of a display on the display unit during measurement. [Figure 8] FIG. 4 is a diagram for explaining switching of the display on the display unit. [Figure 9] FIG. 1 is a diagram (flowchart) for explaining an electrophoretic analysis method according to one embodiment. DETAILED DESCRIPTION OF THE INVENTION

[0012] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will now be described with reference to the accompanying drawings.

[0013] (Overall configuration of the electrophoresis system) An electrophoresis system 100 according to one embodiment of the present invention will be described with reference to FIGS.

[0014] 1, an electrophoresis system 100 according to this embodiment includes an electrophoresis apparatus 101 and an analysis apparatus 102. The analysis apparatus 102 is an example of the "computer" in the claims.

[0015] The electrophoresis device 101 separates the measurement targets by electrophoresis using three chips 60a, 60b, and 60c, thereby measuring (analyzing) components contained in the measurement targets. Specifically, in the electrophoresis device 101, the measurement targets pre-arranged on the plate 70 and the sample placement section 71 (see FIG. 2) are separated by electrophoresis in the flow channels 61 (see FIG. 3) provided in each of the chips 60a to 60b. The electrophoresis device 101 then measures the degree of separation (mobility) of the measurement targets separated by electrophoresis. The chips 60a to 60c are an example of a "flow channel member" in the claims.

[0016] <Configuration of Electrophoresis Apparatus> As shown in FIGS. 1 and 2, the electrophoresis apparatus 101 includes a supply unit 10, a voltage application unit 20, a measurement unit 30, and a control unit 40.

[0017] In the electrophoresis apparatus 101, the supply unit 10 operates to supply the measurement target and a separation buffer to the flow channels 61 of the chips 60a, 60b, and 60c in order to perform electrophoretic measurement.

[0018] The measurement object includes, for example, DNA (Deoxyribonucleic acid), RNA (Ribonucleic acid), or protein. The measurement object includes a sample for measuring the degree of separation (mobility) of each component by electrophoresis, and a reference sample that serves as a reference for measuring the sample by electrophoresis. The sample is a measurement object for which the degree of separation by electrophoresis (measurement waveform), which is the measurement value 111 (see FIG. 4) measured by the measurement unit 30 described below, is unknown. The reference sample is a measurement object containing nucleic acid or protein for which separation characteristics such as molecular weight (chain length) are already known.

[0019] The measurement targets are placed on a plate 70 and a sample placement unit 71. The plate 70 has a plurality of wells 70a, which are multiple placement positions where the measurement targets are placed. For example, the plate 70 has 96 wells 70a arranged in an 8×12 arrangement. The plate 70 is placed at a plate placement position inside the electrophoresis apparatus 101 by an operator, with multiple types of measurement targets placed in all or some of the multiple wells 70a. The measurement targets are placed in the sample placement unit 71 separately from the plate 70. The sample placement unit 71 has a plurality of placement positions where the measurement targets are placed, and the multiple placement positions in the sample placement unit 71 are referred to as wells 71a. For example, a measurement target whose measurement value 111 is unknown and which will be analyzed is placed in the well 70a of the plate 70, and a reference sample whose measurement value 111 is known is placed in the well 71a of the sample placement unit 71 separately from the plate 70.

[0020] The separation buffer is a separation medium that is filled into each of the flow channels 61 (see FIG. 3) of the chips 60a, 60b, and 60c before the measurement target is supplied. The separation buffer contains, for example, at least one of a pH buffer material and a water-soluble polymer (such as a cellulose-based polymer). The separation buffer is filled into a buffer container (not shown). The separation buffer may be placed on the plate 70 or the sample placement section 71. In the electrophoresis device 101, the measurement target is supplied and electrophoresis is performed in a state in which the flow channels 61 are filled with the separation buffer in advance.

[0021] 2, the supply unit 10 has a probe 11 and a pump 12. The supply unit 10 moves the probe 11 to supply the separation buffer and the measurement targets (sample and reference sample) placed on the plate 70 or the sample placement unit 71 to the chips 60a to 60c. The pump 12 adjusts the pressure for the probe 11 to suck in and discharge the separation buffer and the measurement targets.

[0022] 3, chips 60a to 60c each have a channel 61 therein. Chips 60a, 60b, and 60c have the same configuration. In the following description, chip 60a will be illustrated and described in detail, and chips 60b and 60c will not be described because they are similar to chip 60a.

[0023] Chip 60a is a microchip for electrophoresis in which a channel 61 for performing electrophoresis is provided inside a pair of combined flat plate-like members. Channel 61 includes a separation channel 62 and a preparation channel 63. Separation channel 62 and preparation channel 63 are provided so as to intersect with each other. Separation channel 62 is provided to separate the object to be measured by electrophoresis. Furthermore, preparation channel 63 is provided to guide the object to be measured to separation channel 62.

[0024] Furthermore, reservoir sections 64a and 64b, which are spaces for supplying and suctioning the separation buffer and the measurement target, are provided at both ends of the preparation flow channel 63. Similarly, reservoir sections 64c and 64d are provided at both ends of the separation flow channel 62. A plurality of electrodes 65a, 65b, 65c, and 65d are arranged in the flow channel 61. Specifically, electrodes 65a and 65b are arranged in the reservoir sections 64a and 64b provided at both ends of the preparation flow channel 63, respectively. Electrodes 65c and 65d are arranged in the reservoir sections 64c and 64d provided at both ends of the separation flow channel 62, respectively.

[0025] In the electrophoresis device 101, electrophoresis is performed by applying a voltage from a voltage application unit 20 to a plurality of electrodes 65a to 65d provided in a flow path 61. The magnitude of the voltage applied to the electrodes 65a to 65d is controlled by a control unit 40. Three voltage application units 20 (see FIG. 2) are provided corresponding to the chips 60a to 60c, respectively, so as to apply a DC voltage to the flow path 61 of each of the chips 60a to 60c. That is, a DC voltage is applied to the flow path 61 of the chips 60b and 60c by the voltage application unit 20, just like the chip 60a.

[0026] In the electrophoresis apparatus 101, when performing electrophoretic measurement in the chip 60a, first, the supply unit 10 fills the entire interior of the channel 61 (the separation channel 62 and the preparation channel 63) with a separation buffer. Then, the measurement object aspirated from, for example, a predetermined well 70a of a plate 70 is supplied to the reservoir 64a of the preparation channel 63 by the supply unit 10. Then, the voltage application unit 20 applies a predetermined voltage to the electrodes 65a to 65d, causing the measurement object to move inside the preparation channel 63 and to move to a position where the preparation channel 63 and the separation channel 62 intersect. Thereafter, the magnitude of the voltage applied to each of the electrodes 65a to 65d by the voltage application unit 20 is changed, causing the measurement object to move toward the electrode 65d (reservoir 64d) while being separated inside the separation channel 62 by electrophoresis.

[0027] At this time, in separation measurement by electrophoresis, the measurement target moves inside separation channel 62 at different speeds for each component contained in the measurement target, depending on the separation characteristics such as the molecular weight (chain length) of the component contained. In electrophoresis device 101, the components that arrive in order are measured at measurement position 66 in separation channel 62, thereby measuring the separation characteristics for each component of the measurement target. In this way, in electrophoresis device 101, the components contained in the measurement target are measured for each degree of separation (degree of migration).

[0028] As shown in FIG. 2, in this embodiment, the measurement unit 30 measures measurement targets separated by electrophoresis in each of the channels 61 of multiple (three) chips 60a to 60c. For example, the measurement unit 30 detects fluorescence of components of the measurement targets separated by electrophoresis. The measurement unit 30 has an LED 31 (light-emitting diode) that irradiates a measurement position 66 (see FIG. 3) of the separation channel 62 with excitation light. The excitation light from the LED 31 is irradiated onto each component of the measurement targets moving through the separation channel 62 while being separated by electrophoresis, and the component is excited to emit fluorescence. The measurement unit 30 measures this fluorescence using a photomultiplier tube 32, for example, via an optical fiber and a filter member, thereby measuring the degree of separation of the measurement targets by electrophoresis.

[0029] 4, photomultiplier tube 32 outputs a measurement signal indicating a measurement value 111 to control unit 40 in accordance with the intensity of the detected fluorescence. Measurement value 111 based on measurement by measurement unit 30 indicates a large value (peak) at the timing when the object to be measured, which is moving while being separated by electrophoresis, passes measurement position 66 (see FIG. 3). As a result, the amount and composition of each component contained in the object to be measured are analyzed based on the magnitude and position (timing) of the peak for each component contained in the object to be measured.

[0030] The electrophoresis apparatus 101 is provided with a washing mechanism (not shown). The electrophoresis apparatus 101 is configured to repeatedly perform measurements using each of the chips 60a to 60c multiple times by washing the measurement target and separation buffer remaining in the flow channel 61 with the washing mechanism every time one measurement target is measured in each portion including the chips 60a to 60c and the supply unit 10.

[0031] 2 and 3, the electrophoresis device 101 also includes a current detection unit 21 and a voltage detection unit 22. The current detection unit 21 and the voltage detection unit 22 respectively detect the current and voltage output from each of the multiple (three) voltage application units 20. The current detection unit 21 detects the current flowing through each of the flow paths 61 of the chips 60a to 60c due to the voltage applied by the voltage application unit 20. The voltage detection unit 22 detects the voltage applied by the voltage application unit 20 to each of the flow paths 61 of the chips 60a to 60c.

[0032] For example, in chip 60a, current detection unit 21 detects the current flowing through each of electrodes 65a, 65b, 65c, and 65d, thereby detecting the current flowing through flow path 61. Similarly, voltage detection unit 22 detects the voltage applied to each of electrodes 65a, 65b, 65c, and 65d, thereby detecting the voltage applied to flow path 61. Then, current detection unit 21 and voltage detection unit 22 output detection signals indicating the detected current value 112 (see FIG. 5) and voltage detection value 113 (see FIG. 6), respectively, to control unit 40. The same applies to chip 60b and chip 60c.

[0033] 5 and 6, the control unit 40 is configured to acquire four detected current values ​​112 and four detected voltage values ​​113 corresponding to the four electrodes 65a to 65d for each chip 60a (60b or 60c). At time T1 shown in FIGS. 5 and 6, the detected current values ​​112 and the detected voltage values ​​113 change significantly. This means that at time T1, the period during which the measurement object is moved in the preparation channel 63 is switched to the period during which the measurement object is moved in the separation channel 62.

[0034] The control unit 40 controls the operation of each part of the electrophoresis apparatus 101. The control unit 40 is, for example, a microcomputer (microcontroller) having a processing device such as a CPU (Central Processing Unit) and a storage device such as a flash memory. The control unit 40 also includes a communication module and is configured to be able to communicate with the analysis device 102. Based on a drive signal from the analysis device 102, the control unit 40 controls the operation of each part of the electrophoresis apparatus 101 so as to sequentially perform electrophoretic measurements of the measurement targets placed on the plate 70 and the sample placement unit 71.

[0035] Specifically, the control unit 40 operates the supply unit 10 based on a drive signal from the analyzer 102, thereby sequentially supplying the measurement targets arranged in the wells 70a of the plate 70 to each of the chips 60a-60c so that one type of measurement is performed for each chip 60a-60c. The control unit 40 then applies a voltage to the flow paths 61 of the chips 60a-60c using the voltage application unit 20, thereby separating (moving) the measurement targets by electrophoresis. The control unit 40 also acquires measurement values ​​111 (electropherograms) measured by the measurement units 30 provided corresponding to each of the chips 60a-60c. The control unit 40 then acquires the measurement values ​​111 for each of the multiple wells 70a of the plate 70 and for each well 71a of the sample placement unit 71. The control unit 40 is also configured to acquire a current detection value 112 detected by the current detection unit 21 and a voltage detection value 113 detected by the voltage detection unit 22 in synchronization with the measurement by the measurement unit 30.

[0036] Then, for each of chips 60a to 60c, the control unit 40 outputs in real time to the analytical device 102 the measurement value 111 of the measurement target measured by the measurement unit 30, the current detection value 112 of the current detected by the current detection unit 21, and the voltage detection value 113 of the voltage detected by the voltage detection unit 22, over the period from the period when the measurement target is introduced (migrated) in the preparation flow path 63 to the period when the measurement target is being measured in the separation flow path 62.

[0037] <Configuration of the analysis device> 1, the analysis device 102 includes an operation unit 51, a display unit 52, a storage unit 53, and a control unit 54. The analysis device 102 is a computer for analyzing measurement values ​​111 measured by the electrophoresis device 101. The analysis device 102 is configured to be able to communicate with the electrophoresis device 101, and is configured to acquire each of the measurement values ​​111, detected current values ​​112, and detected voltage values ​​113 acquired by the electrophoresis device 101.

[0038] The operation unit 51 accepts input operations by an operator. The operation unit 51 also outputs an operation signal based on the accepted input operation to the control unit 54. The operation unit 51 is, for example, a keyboard and a pointing device such as a mouse.

[0039] The display unit 52 is, for example, a monitor such as a liquid crystal display, and displays the input information under the control of the control unit 54.

[0040] Storage unit 53 is configured with a storage device such as a hard disk drive or an SSD (Solid State Drive). Storage unit 53 stores measured values ​​111, detected current values ​​112, and detected voltage values ​​113 acquired by electrophoresis device 101. Storage unit 53 also stores electrophoretic analysis program 53a for operating control unit 54. Storage unit 53 also stores various parameters such as preset settings or settings (measurement conditions) input by the operator.

[0041] The control unit 54 is a computer including a CPU, a RAM (Random Access Memory), a ROM (Read Only Memory), etc. The control unit 54 controls each part of the analysis device 102 by executing a program (electrophoresis analysis program 53a) stored in the storage unit 53. The control unit 54 is also configured to be able to communicate with the control unit 40 of the electrophoresis device 101 via a communication module (not shown).

[0042] (Details of control by analytical equipment) The control unit 54 transmits an operating signal to the control unit 40 to operate the electrophoresis apparatus 101. Specifically, the control unit 54 acquires various parameters for performing electrophoresis based on an input operation received by the operation unit 51. For example, based on the input operation on the operation unit 51, the control unit 54 acquires well information 52b (see FIG. 8) indicating the wells 70a and 71a in which the measurement targets to be measured are placed, measurement condition information including information indicating the magnitude and time of the applied voltage, and schedule information 52c (see FIG. 8) indicating the measurement order of the measurement targets placed in the multiple wells 70a and 71a. Note that the well information 52b, measurement condition information, and schedule information 52c may be selected from a database previously stored in the storage unit 53. The control unit 54 then transmits a drive signal including the acquired well information 52b, measurement condition information, and schedule information 52c to the control unit 40 of the electrophoresis apparatus 101. Then, the control unit 54 acquires each of the measurement value 111, current detection value 112, and voltage detection value 113 obtained by control by the control unit 40 based on the transmitted drive signal in real time from the control unit 40 as the measurement progresses.

[0043] 7, in this embodiment, control unit 54 is configured to cause display unit 52 to display measured values ​​111, detected current values ​​112, and detected voltage values ​​113. Specifically, control unit 54 is configured to cause display unit 52 to display, during measurement of the object to be measured, time-series values ​​of measured values ​​111, time-series values ​​of four detected current values ​​112 corresponding to each of the currents flowing through each of the plurality of electrodes 65a to 65d, and time-series values ​​of four detected voltage values ​​113 corresponding to each of the voltages applied to each of the plurality of electrodes 65a to 65d.

[0044] In detail, the control unit 54 displays the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 on the display unit 52 while updating them in real time during the measurement of the measurement object. The control unit 54 is configured to display the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 on the display unit 52 not only while the measurement of the measurement object is being performed (while the measurement object is being moved to the separation flow path 62 and measurement is being performed by the measurement unit 30), but also while the measurement object is being introduced into the preparation flow path 63. The control unit 54 is also configured to display the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 acquired over a common measurement period as waveforms on the display unit 52.

[0045] That is, the control unit 54 is configured to, after supplying the measurement object to the reservoir portion 64a in each chip 60a (60b or 60c), display on the display unit 52 the time series values ​​of the measurement value 111, the current detection value 112, and the voltage detection value 113 from the time when the voltage application unit 20 started applying the DC voltage to the present.

[0046] The control unit 54 is also configured to display, on the display unit 52, time-series values ​​(waveforms) of four detected current values ​​112 corresponding to each of the currents flowing through the electrodes 65a-65d and time-series values ​​(waveforms) of four detected voltage values ​​113 corresponding to each of the voltages applied to the electrodes 65a-65d, superimposed on each other in different colors so that they can be distinguished from one another. For example, the control unit 54 displays the time-series values ​​of the detected current values ​​112 and the detected voltage values ​​113 corresponding to the electrode 65a as a red waveform. The control unit 54 displays the time-series values ​​of the detected current values ​​112 and the detected voltage values ​​113 corresponding to the electrode 65b as a yellow waveform. The control unit 54 displays the time-series values ​​of the detected current values ​​112 and the detected voltage values ​​113 corresponding to the electrode 65c as a blue waveform. The control unit 54 displays the time-series values ​​of the detected current values ​​112 and the detected voltage values ​​113 corresponding to the electrode 65d as a green waveform. In FIG. 7 (and FIG. 8), the red waveform, yellow waveform, blue waveform, and green waveform are shown by a solid line, a dotted line, a dashed line, and a two-dot dashed line, respectively.

[0047] Then, the control unit 54 causes the display unit 52 to display a waveform indicating the time-series values ​​of the measured values ​​111, a waveform indicating the time-series values ​​of the detected voltage values ​​113, and a waveform indicating the time-series values ​​of the detected current values ​​112, arranged horizontally (left to right) in this order. The control unit 54 is also configured to cause the display unit 52 to display the time-series values ​​(waveforms) of the measured values ​​111, the detected current values ​​112, and the detected voltage values ​​113 for each of the three chips 60a, 60b, and 60c.

[0048] Specifically, the control unit 54 assigns the number 1 to chip 60a, the number 2 to chip 60b, and the number 3 to chip 60c, and arranges chips 60a, 60b, and 60c in this order vertically, and causes the display unit 52 to display the time-series values ​​(waveforms) of the respective measured values ​​111, detected current values ​​112, and detected voltage values ​​113. That is, the control unit 54 causes the display unit 52 to display in real time the measured values ​​111, detected current values ​​112, and detected voltage values ​​113 obtained by measurement on each of the three chips 60a to 60c currently being analyzed. The control unit 54 also causes the display unit 52 to display a display indicating the position of the well 70a or 71a corresponding to the measurement target currently being measured on each of the three chips 60a to 60c.

[0049] For example, in the example shown in Figure 7, the time series values ​​(waveforms) of the measurement value 111, current detection value 112, and voltage detection value 113 obtained while analyzing (measuring) the measurement object placed in well 71a at position X1A (see Figure 8) of the sample placement section 71 using chip number 1 60a, chip number 2 60b, and chip number 3 60c are displayed.

[0050] 8, control unit 54 is configured to display well information 52b and schedule information 52c on display unit 52 in addition to the time-series values ​​of measurement value 111, detected current value 112, and detected voltage value 113. The time-series values ​​of measurement value 111, detected current value 112, and detected voltage value 113 are displayed with the horizontal axis representing the elapsed time from the time when voltage application by voltage application unit 20 started, with 0 second as the time.

[0051] 7 and 8 , in this embodiment, the control unit 54 is configured to switch between displaying the time-series values ​​of the measured values ​​111, the detected current values ​​112, and the detected voltage values ​​113 on the display unit 52 and displaying well information 52b and schedule information 52c on the display unit 52 in addition to the time-series values ​​of the measured values ​​111, the detected current values ​​112, and the detected voltage values ​​113, based on a switching operation received by the operation unit 51. Specifically, the control unit 54 is configured to switch the display on the display unit 52 when a switching operation to switch the display is received, such as by clicking a display switching button 52a displayed at the top of the display unit 52. That is, the control unit 54 is configured to switch the display on the display unit 52 between the state shown in FIG. 7 and the state shown in FIG. 8 based on a switching operation received by the operation unit 51.

[0052] The well information 52b is displayed in the upper left position on the display unit 52. The well information 52b is information indicating the multiple wells 70a of the plate 70 and the multiple wells 71a of the sample placement unit 71. Specifically, the control unit 54 causes the display unit 52 to display the well information 52b, including information indicating which of the wells 70a of the plate 70 and the wells 71a of the sample placement unit 71 the measurement target is placed in. In other words, the control unit 54 causes the well information 52b to display information indicating the wells 70a and 71a in which analysis (measurement) will be performed. In the well information 52b, the wells 70a and 71a in which measurement will be performed are displayed in a color (e.g., blue) different from the wells 70a and 70b in which measurement will not be performed. The wells 70a and 71a in which the measurement target is placed are set, for example, based on an input operation by the operator.

[0053] Schedule information 52c is displayed in the upper right corner of display unit 52. Schedule information 52c is information indicating the order in which measurements by electrophoresis using chips 60a-60c will be performed on measurement targets placed in multiple wells 70a and 71a. Control unit 54 displays, at the bottom of display unit 52, time-series values ​​(waveforms) of measured values ​​111, detected current values ​​112, and detected voltage values ​​113 of measurement targets currently being measured on chips 60a-60c, and also displays measurement targets scheduled to be measured on chips 60a-60c in order of measurement, starting from the top, along with information indicating the wells 70a and 71a in which they are placed.

[0054] In addition to the measurement order, the control unit 54 indicates in the schedule information 52c which of the chips 60a to 60c will be used to measure the measurement targets placed in the target wells 70a and 71a by using chip numbers (1 to 3). The control unit 54 also indicates in the schedule information 52c information indicating the type of measurement target placed in the corresponding wells 70a and 71a. As described above, the measurement targets include two types: a reference sample that serves as a reference and a sample whose degree of separation (waveform of the measurement value 111) is unknown. In the schedule information 52c, the control unit 54 displays the reference sample as a "sample standard" and the sample as a "sample" on the display unit 52 so that the type of measurement target can be identified.

[0055] (Electrophoretic analysis method) Next, an electrophoretic analysis method using electrophoresis system 100 according to this embodiment will be described with reference to Fig. 9. The control processing in steps 201 to 208 is performed by control unit 54 (analyzer 102) executing electrophoretic analysis program 53a stored in storage unit 53.

[0056] First, in step 201, measurement condition information for performing measurement is acquired. Specifically, well information 52b indicating wells 70a and 71a in which measurement targets to be measured are placed, schedule information 52c indicating the measurement order, and information indicating the type of measurement targets placed in wells 70a and 71a are acquired (set) together with measurement condition information including information indicating the magnitude and time of the voltage applied by voltage application unit 20. Note that this information may be acquired based on an input operation to operation unit 51, or may be acquired from information previously stored in memory unit 53 or the like.

[0057] Next, in step 202, a drive signal including the acquired measurement condition information is transmitted to the control unit 40 of the electrophoresis device 101. The drive signal includes the acquired well information 52b and schedule information 52c in addition to the measurement condition information. Based on this drive signal, in the electrophoresis device 101, analysis (measurement) by electrophoresis using chips 60a to 60c is performed for each measurement target in predetermined wells 70a and 71a in the set order.

[0058] Next, in step 203, measurement values ​​111 are obtained based on measurements by the measurement unit 30 of the electrophoresis apparatus 101. The measurement values ​​111 are sequentially obtained in real time as the measurements proceed. Specifically, the measurement object placed in one of the wells 70a and 71a corresponding to the preset measurement order is supplied to one of the chips 60a to 60c, and the voltage application unit 20 starts applying a voltage. As a result, measurement values ​​111 obtained by the measurement unit 30 are obtained in real time from the point in time when the introduction of the measurement object into the preparation flow path 63 is started in one of the chips 60a to 60c.

[0059] In step 204, current detection values ​​112 of the current flowing through the flow path 61 are obtained due to the voltage applied by the voltage application unit 20 of the electrophoresis device 101. Specifically, four current detection values ​​112 of the current flowing through each of the electrodes 65a to 65d are obtained in real time in synchronization with the measurement values ​​111 by detection by the current detection unit 21.

[0060] In step 205, voltage detection values ​​113 of the voltages applied to flow channel 61 by voltage application unit 20 of electrophoresis apparatus 101 are acquired. Specifically, four voltage detection values ​​113 of the voltages applied to electrodes 65a to 65d, detected by voltage detection unit 22, are acquired in real time in synchronization with measurement values ​​111. Note that acquisition of measurement values ​​111, current detection values ​​112, and voltage detection values ​​113 in steps 203 to 205 is performed at approximately the same timing.

[0061] Next, in step 206, the time-series values ​​(waveforms) of the acquired measured value 111, detected current value 112, and detected voltage value 113 are displayed on display unit 52. The time-series values ​​of measured value 111, detected current value 112, and detected voltage value 113 are displayed side by side as waveforms spanning a common measurement period from the time when voltage application by voltage application unit 20 began to the present. Note that the acquisition and display of measured value 111, detected current value 112, and detected voltage value 113 in steps 203 to 206 are updated in real time while the measurement object is being introduced into preparation flow path 63 and while the measurement object is being measured.

[0062] Next, in step 207, it is determined whether a switching operation for switching the display has been accepted. If it is determined that a switching operation has been accepted, the process proceeds to step 208. If it is not determined that a switching operation has been accepted, the control process is terminated.

[0063] In step 208, based on the accepted switching operation, the display unit 52 is switched between displaying the time series values ​​of the measurement value 111, the current detection value 112, and the voltage detection value 113, and displaying the well information 52b and the schedule information 52c in addition to the time series values ​​of the measurement value 111, the current detection value 112, and the voltage detection value 113 on the display unit 52.

[0064] The control processing in steps 203 to 208 is performed until measurement (analysis) of the measurement target placed in the predetermined well 70a or 71a in one chip 60a (60b or 60c) is completed. Then, when measurement (analysis) of the measurement target placed in the predetermined well 70a or 71a is completed, measurement is performed on a new measurement target placed in the next well 70a or 71a in accordance with the schedule information 52c. Furthermore, measurements in each of the chips 60a to 60c are performed simultaneously.

[0065] (Effects of this embodiment) In this embodiment, the following effects can be obtained.

[0066] In the electrophoresis system 100 of this embodiment, as described above, the display unit 52 displays the time-series values ​​of the measured values ​​111 and the time-series values ​​of the detected current values ​​112 while measuring the object to be measured. If an abnormality occurs in the measured values ​​111 and an abnormality also occurs in the current (detected current values ​​112), this may indicate an abnormality in the concentration of the object to be measured itself, an abnormality in the power supply parts (such as the voltage application unit 20, wiring members, or electrodes 65a-65d) that supply the current, or an abnormality in the process of filling the flow channel 61 with the object to be measured (an abnormality in the supply unit 10). On the other hand, if an abnormality occurs in the measured values ​​111 but no abnormality occurs in the current (detected current values ​​112), this may indicate deterioration of the members (chips 60a-60c) that form the flow channel 61. Therefore, as described above, by displaying the time-series values ​​of the measurement value 111 and the time-series values ​​of the detected current value 112 on the display unit 52 while measuring the object to be measured, it is possible to visually check the display unit 52 to see whether an abnormality has occurred in either the measurement value 111 or the current (detected current value 112). Therefore, if an abnormality has occurred in the measurement value 111, it is possible to check whether an abnormality has occurred in the current. This allows the cause of the abnormality in the measurement value 111 to be narrowed down to possible abnormalities such as an abnormality in the concentration of the object to be measured itself, an abnormality in the power supply parts (such as the voltage application unit 20, wiring members, or electrodes 65a-65d) for supplying the current, an abnormality in the process of filling the flow path 61 with the object to be measured (an abnormality in the supply unit 10), or deterioration of the members (chips 60a-60c) forming the flow path 61. As a result, when an abnormality has occurred in the measurement value 111 measured by electrophoresis, the workload on the operator for determining the cause of the abnormality can be reduced.

[0067] Furthermore, in the above embodiment, further effects can be obtained by configuring as follows.

[0068] That is, in this embodiment, as described above, electrophoresis system 100 includes analyzer 102 that acquires measurement values ​​111 and detected current values ​​112, and analyzer 102 (controller 54) is configured to display the time-series values ​​of measurement values ​​111 and detected current values ​​112 on display 52 while updating them in real time during measurement of the object to be measured. With this configuration, analyzer 102 displays the time-series values ​​of measurement values ​​111 and detected current values ​​112 on display 52 while updating them in real time. Therefore, by visually checking display 52, an operator can confirm the temporal changes between the latest measurement values ​​111 and detected current values ​​112, which are updated in real time. This allows the operator to more easily check whether an abnormality has occurred in measurement values ​​111 and detected current values ​​112. As a result, because it is easier to check whether an abnormality has occurred in the current, the workload on the operator for determining the cause of the abnormality in measurement values ​​111 can be further reduced.

[0069] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 includes a voltage detection unit 22 that detects the voltage applied to the flow path 61, and the analyzer 102 (control unit 54) is configured to display on the display unit 52 time-series values ​​of the measured value 111 and the detected current value 112, as well as time-series values ​​of the voltage detected by the voltage detection unit 22 during measurement of the object to be measured. With this configuration, the operator can check the time-series values ​​of the measured value 111 and the detected current value 112 as well as the time-series values ​​of the detected voltage value 113 during measurement. Here, if an abnormality occurs in the voltage, it is possible that an abnormality has occurred not in the object to be measured itself or the components (chips 60a-60c) forming the flow path 61, but in the components for passing the current (such as the voltage application unit 20, wiring components, or electrodes 65a-65d). Therefore, by checking the measured value 111, the detected current value 112, and the detected voltage value 113, the operator can further narrow down the type of cause of the abnormality in the measured value 111. As a result, when an abnormality occurs in the measured value 111 measured by electrophoresis, the workload of the operator to determine the cause of the abnormality can be further reduced.

[0070] Furthermore, in this embodiment, as described above, the analyzer 102 (controller 54) is configured to display the time series values ​​of the measured values ​​111 and the detected current values ​​112 over a common measurement period as waveforms on the display unit 52. This configuration makes it possible to easily compare the timing at which an abnormality occurs in the measured values ​​111 with the timing at which an abnormality occurs in the detected current values ​​112. Therefore, when an abnormality occurs in the measured values ​​111, the operator can more intuitively confirm whether or not an abnormality occurs in the detected current values ​​112. As a result, the workload on the operator to determine the cause of an abnormality when an abnormality occurs in the measured values ​​111 can be further reduced.

[0071] Furthermore, in this embodiment, as described above, the analyzer 102 (controller 54) is configured to display the time-series values ​​of the measured values ​​111 and the detected current values ​​112 side by side on the display unit 52. Here, the measured values ​​111 of electrophoresis change so that their value increases each time a component of the separated measurement target is detected, resulting in a waveform with multiple peaks over time. On the other hand, because the current detected in electrophoresis is a substantially constant direct current, the time-series values ​​of the detected current values ​​112 exhibit substantially constant values. Therefore, if the measured values ​​111 and the detected current values ​​112, which have different shapes, are displayed superimposed, it is considered that the visibility of both the measured values ​​111 and the detected current values ​​112 will be reduced. In contrast, in this embodiment, the time-series values ​​of the measured values ​​111 and the detected current values ​​112 are displayed side by side on the display unit 52, thereby preventing a reduction in the visibility of the measured values ​​111 and the detected current values ​​112.

[0072] Furthermore, in this embodiment, as described above, the analyzer 102 (controller 54) is configured to display on the display unit 52 well information 52b indicating the multiple wells 70a and 71a, which are the multiple placement positions of the measurement targets, and schedule information 52c indicating the measurement order of the measurement targets placed in each of the multiple wells 70a and 71a, in addition to the time-series values ​​of the measurement values ​​111 and the detected current values ​​112. With this configuration, since the well information 52b and schedule information 52c are displayed in addition to the measurement values ​​111 and the detected current values ​​112, the operator can confirm information about the measurement target currently being measured. Therefore, if an abnormality occurs in the measurement values ​​111, it can easily be confirmed which well 70a or 71a is being measured for the measurement target that is causing the abnormality.

[0073] Furthermore, in this embodiment, as described above, the analyzer 102 (controller 54) is configured to switch between displaying the time-series values ​​of the measured values ​​111 and the detected current values ​​112 on the display unit 52 and displaying well information 52b and schedule information 52c on the display unit 52 in addition to the time-series values ​​of the measured values ​​111 and the detected current values ​​112, based on a switching operation received by the operation unit 51, which receives input operations by the operator. With this configuration, the operator can easily switch between displaying the measured values ​​111 and the detected current values ​​112 and displaying well information 52b and schedule information 52c in addition to the measured values ​​111 and the detected current values ​​112, by operating the operation unit 51. Furthermore, by switching from a state in which well information 52b and schedule information 52c are displayed in addition to the measured values ​​111 and the detected current values ​​112 to a state in which only the measured values ​​111 and the detected current values ​​112 are displayed, the measured values ​​111 and the detected current values ​​112 can be displayed in an enlarged scale. Therefore, by operating the operation unit 51, the visibility of the measured value 111 and the detected current value 112 can be easily improved.

[0074] Furthermore, in this embodiment, as described above, the current detection unit 21 detects the current flowing through each of the multiple electrodes 65a-65d arranged in the flow path 61, and the analyzer 102 (controller 54) is configured to display, on the display unit 52, time-series values ​​of the multiple (four) current detection values ​​112 corresponding to the currents flowing through each of the multiple (four) electrodes 65a-65d, superimposed on each other in a distinguishable manner using different colors. This configuration allows the operator to easily visually distinguish and confirm the currents flowing through each of the multiple (four) electrodes 65a-65d. Therefore, the operator can distinguish and confirm the current detection values ​​112 of the detected currents in more detail.

[0075] In this embodiment, as described above, the flow path 61 includes a preparation flow path 63 for guiding the measurement target to the separation flow path 62, the current detection unit 21 detects the current flowing through each of the plurality of electrodes 65a-65d arranged at both ends (reservoir sections 64c and 64d) of the separation flow path 62 and at both ends (reservoir sections 64a and 64b) of the preparation flow path 63, and the analyzer 102 (controller 54) is configured to display, on the display unit 52, time-series values ​​of the plurality of (four) detected current values ​​112 corresponding to the currents flowing through each of the plurality of electrodes 65a-65d and time-series values ​​of the measured values ​​111 while measuring the measurement target and while introducing the measurement target into the preparation flow path 63. With this configuration, the detected current values ​​112 can be confirmed not only while the measurement target is being separated and measured by electrophoresis, but also when an abnormality occurs while introducing the measurement target into the preparation flow path 63. Therefore, when an abnormality occurs in the measurement value 111, the operator can check whether or not the abnormality occurred in the preparation stage for the measurement.

[0076] Furthermore, in this embodiment, as described above, the measurement unit 30 measures the measurement target separated by electrophoresis in each of the flow paths 61 of the plurality of chips 60a to 60c (flow path members) having the flow paths 61 provided therein, the current detection unit 21 detects the current flowing in each of the flow paths 61 of the plurality of chips 60a to 60c, and the analyzer 102 (control unit 54) is configured to display the time series values ​​of the measurement values ​​111 and the detected current values ​​112 for each of the plurality of chips 60a to 60c on the display unit 52. With this configuration, even when measurements are being performed simultaneously in the flow paths 61 of the plurality of chips 60a to 60c, the operator can easily distinguish and check whether or not an abnormality has occurred in each of the flow paths 61.

[0077] (Effects of the electrophoretic analysis method and electrophoretic analysis program according to this embodiment) The electrophoretic analysis method and electrophoretic analysis program 53a of this embodiment can provide the following effects.

[0078] The electrophoretic analysis method and electrophoretic analysis program 53a of this embodiment, configured as described above, display unit 52 displays time-series values ​​of measured values ​​111 and time-series values ​​of detected current values ​​112 during measurement of the object to be measured. If an abnormality occurs in measured values ​​111 and an abnormality also occurs in the current (detected current value 112), this may indicate an abnormality in the concentration of the object to be measured itself, an abnormality in the power supply parts (voltage application unit 20, wiring members, electrodes 65a-65d, etc.) for supplying the current, or an abnormality in the process of filling flow path 61 with the object to be measured (an abnormality in supply unit 10). On the other hand, if an abnormality occurs in measured values ​​111 but no abnormality occurs in the current (detected current value 112), this may indicate deterioration of the members (chips 60a-60c) forming flow path 61. Therefore, as described above, by displaying the time-series values ​​of measurement value 111 and the time-series values ​​of detected current value 112 on display unit 52 while measuring the object to be measured, it is possible to visually check display unit 52 to see whether or not an abnormality has occurred in either measurement value 111 or the current (detected current value 112). Therefore, if an abnormality has occurred in measurement value 111, it is possible to check whether or not an abnormality has occurred in the current. This allows the cause of the abnormality in measurement value 111 to be narrowed down to possible abnormalities such as an abnormality in the concentration of the object to be measured itself, an abnormality in the power supply parts (voltage application unit 20, wiring members, or electrodes 65a-65d, etc.) for passing the current, an abnormality in the process of filling flow path 61 with the object to be measured (an abnormality in supply unit 10), or deterioration of the members (chips 60a-60c) in which flow path 61 is formed. As a result, it is possible to provide an electrophoretic analysis method and electrophoretic analysis program 53a that can reduce the workload of an operator in determining the cause of an abnormality when an abnormality has occurred in measurement value 111 measured by electrophoresis.

[0079] [Variations] The embodiments disclosed herein should be considered to be illustrative and not restrictive in all respects. The scope of the present invention is defined by the claims rather than the above description of the embodiments, and further includes all modifications (variations) within the meaning and scope of the claims.

[0080] For example, in the above embodiment, an example was shown in which electrophoresis device 101, which performs measurements by electrophoresis, and analysis device 102, which displays measured values ​​111, detected current values ​​112, and detected voltage values ​​113, are provided separately, but the present invention is not limited to this. For example, electrophoresis device 101, which performs measurements by electrophoresis, may be configured to display measured values ​​111, detected current values ​​112, and detected voltage values ​​113. Furthermore, a display device (display unit) that displays measured values ​​111, detected current values ​​112, and detected voltage values ​​113 may be provided separately from electrophoresis device 101 and analysis device 102.

[0081] In the above embodiment, an example has been shown in which three time-series values, namely, the measured value 111, the detected current value 112, and the detected voltage value 113, are displayed on the display unit 52, but the present invention is not limited to this. For example, it is also possible to display two time-series values, namely, the measured value 111 and the detected current value 112, without displaying the time-series value of the detected voltage value 113.

[0082] In the above embodiment, the display unit 52 displays the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 over a common measurement period from the time when the voltage application unit 20 starts applying a voltage. However, the present invention is not limited to this. For example, the time-series values ​​of the detected current value 112 and the detected voltage value 113 from the time when the voltage application unit 20 starts applying a voltage may be displayed, and the time-series values ​​of the measured value 111 from the time when electrophoresis in the separation channel 62 starts (time T1 in FIGS. 5 and 6 ) may be displayed. Alternatively, the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 over a common measurement period during measurement of the measurement object (from the time when electrophoresis in the separation channel 62 starts) may be displayed. Alternatively, the measured value 111, the detected current value 112, and the detected voltage value 113 may be displayed so as to show time-series values ​​for different periods.

[0083] Furthermore, in the above embodiment, an example has been shown in which the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 are displayed side by side (left and right), but the present invention is not limited to this. For example, the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 may be displayed side by side (top and bottom). Furthermore, the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113 may be displayed superimposed. In this case, it is preferable to display only the time-series values ​​of the detected current value 112 and the detected voltage value 113 superimposed.

[0084] In the above embodiment, an example was shown in which well information 52b and schedule information 52c were displayed in addition to the time-series values ​​of the measurement value 111, the detected current value 112, and the detected voltage value 113, but the present invention is not limited to this. For example, either well information 52b or schedule information 52c may be displayed together with the time-series values ​​of the measurement value 111, the detected current value 112, and the detected voltage value 113.

[0085] In the above embodiment, the display is switched based on a switching operation received by the operation unit 51, but the present invention is not limited to this. For example, the display may not be switched while well information 52b and schedule information 52c are displayed in addition to the time-series values ​​of the measured value 111, the detected current value 112, and the detected voltage value 113. Alternatively, the display may be switched so as to erase either the well information 52b or the schedule information 52c.

[0086] In the above embodiment, an example was shown in which time-series values ​​of four current detection values ​​112 of the current flowing through each of the multiple electrodes 65a-65d were displayed, but the present invention is not limited to this. For example, the time-series value of one current detection value 112 of the current flowing through each of the multiple electrodes 65a-65d may be displayed. Alternatively, two current detection values ​​112 may be displayed, one for the current in the separation channel 62 and one for the current in the preparation channel 63. Similarly, for the voltage detection values ​​113, one or two voltage detection values ​​113 may be displayed instead of four.

[0087] Furthermore, in the above embodiment, an example was shown in which the chips 60a to 60c (channel members) are provided with the preparation channel 63 for guiding the measurement target to the separation channel 62, but the present invention is not limited to this. For example, the chips 60a to 60c (channel members) may be configured to have only the separation channel 62 without including the preparation channel 63. Furthermore, instead of a shape in which the separation channel 62 and the preparation channel 63 intersect so as to penetrate each other (a cross shape), the preparation channel 63 may be configured to intersect the separation channel 62 in a T-shape.

[0088] In the above embodiment, the electrophoresis device 101 is configured to measure each of a plurality (three) of chips 60a-60c (flow path members), but the present invention is not limited to this. For example, it may be configured to measure one or two chips (flow path members), or it may be configured to measure four or more chips. Furthermore, even when the electrophoresis device 101 is configured to measure each of the three chips 60a-60c (flow path members), it may be configured to specify (select) only one or two chips for measurement.

[0089] In the above embodiment, the electrophoresis device 101 is configured to perform microchip electrophoresis, but the present invention is not limited to this. For example, the electrophoresis device 101 may be configured to perform capillary electrophoresis without using a microchip.

[0090] In the above embodiment, the degree of separation (mobility) of the measurement target is measured by fluorescence detection, but the present invention is not limited to this. For example, the separated components of the measurement target may be detected by coloring with a reagent.

[0091] [Aspect] It will be appreciated by those skilled in the art that the exemplary embodiments described above are examples of the following aspects.

[0092] (Item 1) an electrophoresis device including: a measurement unit that measures the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; and a current detection unit that detects a current flowing in the flow path; an electrophoresis system configured to cause a display unit to display, while the measurement of the object is being performed, time series values ​​of the measurement value of the object measured by the measurement unit and time series values ​​of the current detection value of the current detected by the current detection unit.

[0093] (Item 2) further comprising an analyzer that acquires the measurement value and the current detection value; Item 2. The electrophoresis system according to item 1, wherein the analyzer is configured to display the time series values ​​of the measurement value and the current detection value on the display unit while updating them in real time during measurement of the object to be measured.

[0094] (Item 3) the electrophoresis device further includes a voltage detection unit that detects a voltage applied to the flow channel; 3. The electrophoresis system according to item 1 or 2, wherein the display unit is configured to display, in addition to the time-series values ​​of the measurement values ​​and the current detection values, time-series values ​​of voltage detection values ​​detected by the voltage detection unit while the measurement of the object to be measured is being performed.

[0095] (Item 4) 4. The electrophoresis system according to any one of items 1 to 3, wherein the display unit is configured to display time-series values ​​of the measured values ​​and the detected current values ​​over a common measurement period as waveforms.

[0096] (Item 5) 5. The electrophoresis system according to any one of items 1 to 4, wherein the display unit displays the measured values ​​and the detected current values ​​in time series side by side.

[0097] (Item 6) 6. The electrophoresis system according to any one of items 1 to 5, wherein the display unit is configured to display, in addition to the time-series values ​​of the measured values ​​and the detected current values, well information indicating a plurality of wells at which the measurement targets are placed, and schedule information indicating the measurement order of the measurement targets placed in each of the plurality of wells.

[0098] (Item 7) Item 7. The electrophoresis system according to item 6, wherein the electrophoresis system is configured to switch between displaying the time-series values ​​of the measurement values ​​and the current detection values ​​on the display unit and displaying the well information and the schedule information on the display unit in addition to the time-series values ​​of the measurement values ​​and the current detection values, based on a switching operation received by an operation unit that receives input operations by an operator.

[0099] (Item 8) the current detection unit detects a current flowing through each of a plurality of electrodes arranged in the flow path; 8. The electrophoresis system according to any one of items 1 to 7, wherein the time series values ​​of the current detection values ​​corresponding to the currents flowing through the electrodes are displayed on the display unit in superimposed fashion in different colors so as to be distinguishable from one another.

[0100] (Item 9) The flow path further includes a preparation flow path for guiding the measurement object to the separation flow path, the current detection unit detects a current flowing through each of a plurality of electrodes disposed at both ends of the separation channel and at both ends of the preparation channel; 9. The electrophoresis system according to any one of items 1 to 8, wherein the display unit is configured to display, during measurement of the object to be measured and during introduction of the object to be measured into the preparation flow path, time series values ​​of each of the plurality of current detection values ​​corresponding to each of the currents flowing through each of the plurality of electrodes and time series values ​​of the measurement values.

[0101] (Item 10) the measurement unit measures the measurement object separated by electrophoresis in each of the flow paths of a plurality of flow path members having the flow paths provided therein; the current detection unit detects a current flowing through the flow path of each of the plurality of flow path members, 10. The electrophoresis system according to any one of items 1 to 9, wherein the display unit is configured to display time-series values ​​of the measured values ​​and the detected current values ​​for each of the plurality of flow path members.

[0102] (Item 11) a step of obtaining a measurement value by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; obtaining a current detection value of a current flowing through the flow path; and displaying, on a display unit, time-series values ​​of the measurement values ​​and time-series values ​​of the current detection values ​​while the measurement object is being measured.

[0103] (Item 12) a step of obtaining a measurement value by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; obtaining a current detection value of a current flowing through the flow path; and displaying, on a display unit, time-series values ​​of the measurement values ​​and time-series values ​​of the current detection values ​​while the measurement object is being measured. [Explanation of symbols]

[0104] 21 Current detection section 22 Voltage detection section 30 Measuring part 60a to 60c Chip (flow path member) 61 Flow path 62 Separation channel 63 Preparation channel 65a~65d electrode 100 Electrophoresis System 101 Electrophoresis apparatus 102 Analytical equipment (computer) 111 measurements 112 Current detection value 113 Voltage detection value

Claims

1. an electrophoresis device including: a measurement unit that measures the analytes separated by electrophoresis in a flow path including a separation flow path for separating the analytes and a preparation flow path for guiding the analytes to the separation flow path; and a current detection unit that detects a current flowing in the flow path; an electrophoresis system configured to cause a display unit to display, while the measurement of the object to be measured is being performed in the separation flow path and while the object to be measured is being guided to the separation flow path in the preparation flow path, time series values ​​of the measurement value of the object to be measured measured by the measurement unit and time series values ​​of the current detection value of the current detected by the current detection unit.

2. further comprising an analyzer that acquires the measurement value and the current detection value; 2. The electrophoresis system according to claim 1, wherein the analyzer is configured to display the time series values ​​of the measurement value and the current detection value on the display unit while updating them in real time during measurement of the object to be measured.

3. the electrophoresis device further includes a voltage detection unit that detects a voltage applied to the flow channel; 3. The electrophoresis system according to claim 1, wherein the display unit is configured to display, in addition to the time series values ​​of the measurement values ​​and the current detection values, time series values ​​of voltage detection values ​​of voltages detected by the voltage detection unit while the measurement of the object to be measured is being performed.

4. The electrophoresis system according to any one of claims 1 to 3, wherein the display unit is configured to display time series values ​​of the measurement values ​​and the current detection values ​​over a common measurement period as waveforms.

5. 5. The electrophoresis system according to claim 1, wherein the display unit displays the measured values ​​and the detected current values ​​in time series side by side.

6. an electrophoresis device including: a measurement unit that measures the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; and a current detection unit that detects a current flowing in the flow path; a display unit is configured to display, during measurement of the object to be measured, time series values ​​of the measurement value of the object to be measured measured by the measurement unit and time series values ​​of the current detection value of the current detected by the current detection unit, an electrophoresis system configured to cause the display unit to display, in addition to the time-series values ​​of the measurement values ​​and the current detection values, well information indicating a plurality of wells at which the measurement objects are placed, and schedule information indicating the measurement order of the measurement objects placed in each of the plurality of wells.

7. 7. The electrophoresis system according to claim 6, wherein the electrophoresis system is configured to switch between displaying the time-series values ​​of the measurement values ​​and the current detection values ​​on the display unit and displaying the well information and the schedule information on the display unit in addition to the time-series values ​​of the measurement values ​​and the current detection values, based on a switching operation received by an operation unit that receives an input operation by an operator.

8. an electrophoresis device including: a measurement unit that measures the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; and a current detection unit that detects a current flowing in the flow path; a display unit is configured to display, during measurement of the object to be measured, time series values ​​of the measurement value of the object to be measured measured by the measurement unit and time series values ​​of the current detection value of the current detected by the current detection unit, the current detection unit detects a current flowing through each of a plurality of electrodes arranged in the flow path; an electrophoresis system configured to superimpose and display on the display unit time-series values ​​of the current detection values ​​corresponding to each of the currents flowing through each of the electrodes in different colors so that the values ​​can be distinguished from one another.

9. an electrophoresis device including: a measurement unit that measures the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte; and a current detection unit that detects a current flowing in the flow path; a display unit is configured to display, during measurement of the object to be measured, time series values ​​of the measurement value of the object to be measured measured by the measurement unit and time series values ​​of the current detection value of the current detected by the current detection unit, The flow path further includes a preparation flow path for guiding the measurement object to the separation flow path, the current detection unit detects a current flowing through each of a plurality of electrodes disposed at both ends of the separation channel and at both ends of the preparation channel; an electrophoresis system configured to cause the display unit to display time series values ​​of each of the plurality of current detection values ​​corresponding to each of the currents flowing through each of the plurality of electrodes and time series values ​​of the measurement values ​​while the measurement object is being measured and while the measurement object is being introduced into the preparation flow path.

10. the measurement unit measures the measurement object separated by electrophoresis in each of the flow paths of a plurality of flow path members having the flow paths provided therein; the current detection unit detects a current flowing through the flow path of each of the plurality of flow path members, The electrophoresis system according to any one of claims 1 to 9, wherein the display unit is configured to display time-series values ​​of the measurement values ​​and the current detection values ​​for each of the plurality of flow path members.

11. a step of obtaining a measurement value by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte and a preparation flow path for guiding the analyte to the separation flow path; obtaining a current detection value of a current flowing through the flow path; and displaying, on a display unit, time series values ​​of the measurement values ​​and time series values ​​of the current detection values ​​while the measurement object is being measured in the separation flow path and while the measurement object is being guided to the separation flow path in the preparation flow path.

12. a step of obtaining a measurement value by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte and a preparation flow path for guiding the analyte to the separation flow path; obtaining a current detection value of a current flowing through the flow path; and displaying, on a display unit, time series values ​​of the measurement values ​​and time series values ​​of the current detection values ​​while the measurement object is being measured in the separation flow path and while the measurement object is being guided to the separation flow path in the preparation flow path.

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